RE: AW: [Histonet] overfixation with formalin C.Fox pdf article
Thanks Joelle. I took it from your block. Regards, José Luis Dr. José Luis Palazón Fernández Instituto de Investigaciones Científicas Universidad de Oriente Boca del Rio-Isla Margarita-Venezuela Direccion actual: Instituto de Ciencias Marinas de Andalucia-CSIC Campus universitario Rio San Pedro, 11510, Puerto Real, Cádiz, España email: jluis.pala...@icman.csic.es; jose.pala...@ne.udo.edu.ve tlf: +34-956832612; fax: +34-956834701; cell: +34-600487100 El dia 06 nov 2012 08:54, joelle weaver joellewea...@hotmail.com escribió: Hi everyone- A number of people wanted a copy of the Fox article. Hopefully anyone who emailed me directly got their pdf since I don't think the histonet accepts group attachments. In case anyone else wants this great reference, I posted it on my blog in a post hopefully viewable at the following URL http://histologyconnection.com/?attachment_id=201 [ http://histologyconnection.com/?attachment_id=201 ] Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Sun, 4 Nov 2012 22:23:36 -0300 From: late...@montevideo.com.uy To: Histonet@lists.utsouthwestern.edu Subject: RE: AW: [Histonet] overfixation with formalin C.Fox pdf article CC: Histonet@lists.utsouthwestern.edu On 04/11/2012 at 07:01 p.m. joelle weaver wrote: I will be pleased if someone could send to me a pdf copy of C.Fox article. Thanks in advance, Carlos Defeo Histotechnologist Montevideo,Uruguay http://lists.utsouthwestern.edu/mailman/listinfo/histonet [ http://lists.utsouthwestern.edu/mailman/listinfo/histonet ] ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [ http://lists.utsouthwestern.edu/mailman/listinfo/histonet ] ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [ http://lists.utsouthwestern.edu/mailman/listinfo/histonet ] ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [ http://lists.utsouthwestern.edu/mailman/listinfo/histonet ] ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [ http://lists.utsouthwestern.edu/mailman/listinfo/histonet ] ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [ http://lists.utsouthwestern.edu/mailman/listinfo/histonet ] ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Background staining on H pylori
Deloris, We recently had what we think was a contaminated dispenser of HP from VMS. It had brown particulate matter scattered over the slide which the docs said was not bugs. Received a new dispenser from VMS and have had no problem since. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Deloris Carter Sent: Monday, November 05, 2012 5:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Background staining on H pylori Hi, I'm getting a lot of background staining on my HP's. We use a Ventana BenchmarkXT with ultraView DAB. The problem seems to be escalating of late, and I'm not sure why. We use Hollande's on our GI biopsies, and run them on a shorter run. Nothing has changed in the processing of the specimens, or the IHC procedure. The antibody dispenser is a newer lot, but not brand new, as we get the larger size due to the high volume of HP's we run. Any ideas? Deloris Carter HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Looking at cryostats
What's good out there these days? I am currently looking at the Leica CM3050; are there other brands that I should consider? Thanks so much, Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Subject: [Histonet] Background staining on H pylori
Hey Deloris, I would have the dispensing nozzles on the XT checked, could be it is not getting rinsed properly. Had that issue with my Ultra not to long ago. No warnings or errors showed up, just a clogged nozzle tip. Tim -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Deloris Carter Sent: Monday, November 05, 2012 5:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Background staining on H pylori Hi, I'm getting a lot of background staining on my HP's. We use a Ventana BenchmarkXT with ultraView DAB. The problem seems to be escalating of late, and I'm not sure why. We use Hollande's on our GI biopsies, and run them on a shorter run. Nothing has changed in the processing of the specimens, or the IHC procedure. The antibody dispenser is a newer lot, but not brand new, as we get the larger size due to the high volume of HP's we run. Any ideas? Deloris Carter HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 108, Issue 7 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Looking at cryostats
Not really! For me the Leica is the best. René J. From: kgrob...@rci.rutgers.edu kgrob...@rci.rutgers.edu To: histonet histonet@lists.utsouthwestern.edu Sent: Tuesday, November 6, 2012 9:25 AM Subject: [Histonet] Looking at cryostats What's good out there these days? I am currently looking at the Leica CM3050; are there other brands that I should consider? Thanks so much, Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Looking at cryostats
The CM3050s is the best in my opinion for research or heavy duty frozen sectioning and it can work with the Cryo Jane system or similar if needed. Pam Marcum -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kgrob...@rci.rutgers.edu Sent: Tuesday, November 06, 2012 8:26 AM To: histonet Subject: [Histonet] Looking at cryostats What's good out there these days? I am currently looking at the Leica CM3050; are there other brands that I should consider? Thanks so much, Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] cost per HE
That is quite acceptable cost. Under separate cover I am sending my costs. René J. From: Cheryl tkngfl...@yahoo.com To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Monday, November 5, 2012 10:02 PM Subject: [Histonet] cost per HE Depending on how I dice up labor and materials, I come up with a cost per slide (HE), from receipt of specimen to the slide in the Path's hands ranging everywhere from $6 to $9/ slide. HELP? How do you distill your cost per slide?? Thanks, Cheryl ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Fw: [Histonet] Cytology in Jasper, Indiana
Good Morning Histonetters, We are currently looking for a registered Cytotechnologist to work in a Regional hospital doing all aspects of cytology. The position is full time requiring someone with a clinical Cytology background with processing, screening, assisting on FNA's and learning Cervista HPV HR and Probetec for GC/CT. This is a challenging position with all aspects of cytology and molecular as well. Please contact me directly by e-mail or check mhhcc.org website under careers to apply through the system. Thank You, Thanks Histology/Cytology Supervisor S. Kathy Baldwin, SCT (ASCP) sbald...@mhhcc.org Memorial Hospital and Health Care Center 800 West 9th Str. Jasper, In 47546 Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Looking at cryostats
Leica Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kgrob...@rci.rutgers.edu Sent: Tuesday, November 06, 2012 6:26 AM To: histonet Subject: [Histonet] Looking at cryostats What's good out there these days? I am currently looking at the Leica CM3050; are there other brands that I should consider? Thanks so much, Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Looking at cryostats
Thermo NX70 is very nice. In fact everyone fights to use it rather than the old CM3050 we have... We use them for frozen sections for kidney, skin IF and muscle histochemistry. Lots and lots of frozens... Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kgrob...@rci.rutgers.edu Sent: Tuesday, November 06, 2012 6:26 AM To: histonet Subject: [Histonet] Looking at cryostats What's good out there these days? I am currently looking at the Leica CM3050; are there other brands that I should consider? Thanks so much, Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Looking at cryostats
Leica! Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hor...@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kgrob...@rci.rutgers.edu Sent: Tuesday, November 06, 2012 8:26 AM To: histonet Subject: [Histonet] Looking at cryostats What's good out there these days? I am currently looking at the Leica CM3050; are there other brands that I should consider? Thanks so much, Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Job Opportunity!!!
Medical Center Hospital is a premier center and we currently have opening for Histotechnicain/Histotechnologist in our Department. This is a full time opportunity that will rotate through all area of histology routine procedures, special stains and IHC.Please if interested send your resume to me at Email;tajib...@echd.orgFax no: 432-640 2303 Tel: 432-6402348. Tunde Ajibade BS, HTL(ASCP),QIHC Histology Supervisor Medical Center Hospital Odessa,TX Tel: 432-640-2348 Fax:432-640-2303 CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] SC Society of Histotechnology Meeting This Weekend
Good afternoon Everyone This is just to let you know the SCSHT Meeting will be this weekend November 9-10, 2012 at the beachfront Landmark Resort in Myrtle Beach, SC. 7contact hours available and workshops include Lean Workflow in Histology: Error Reduction Tissue Processing from the Beginning and Histohints to Happiness! You can call or email me for a brochure and registration information. HOPE TO SEE YOU THERE! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: [Histonet] Devasting news on 88305TC component--Where the $18 came from
Sent: Tuesday, November 06, 2012 12:35 PM To: 'histonet-boun...@lists.utsouthwestern.edu' Subject: RE: [Histonet] Devasting news on 88305TC component--Where the $18 came from The $18.00 figure came from an article published in Archives of Pathology and Lab Medicine: Pathology Economic Model Tool A Novel Approach to Workflow and Budget Cost Analysis in an Anatomic Pathology Laboratory David Muirhead, BSc; Patricia Aoun, MD, MPH; Michael Powell, MS, FASHP; Flemming Juncker, MBA; Jens Mollerup, MSc, PhD (Arch Pathol Lab Med. 2010;134:1164-1169) Thanks to Joe Plandowski of IOL for providing me with the reference. Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.492.0203 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webster, Thomas S. Sent: Monday, November 05, 2012 3:01 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Devasting news on 88305TC component I wish there was a way to put a positive spin on this but I can't think of any. We can only hope it kills off client billing somehow. Whomever the stakeholder was that told CMS a typical 88305 costs 18 bucks, I'd love to know how he/she came up with that number. It's insultingly low. http://www.acla.com/sites/default/files/ACLA%20comments%202012%20PFS%20p roposed%20rule%208-30-11_3.pdf I believe whoever it was had the goal to stop the proliferation of POLs. Wouldn't surprise me if they worked for a large national lab that had lost a lot of business to POLs. CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] How to fix frozen section?
Hello, I will receive some frozen tissue sections that are not fixed. I rarely do IHC on frozen sections. I searched some articles about fixation. Some use 50/50 of acetone/alcohol and some use only alcohol. Could you please recommend a fixation method for me? Thanks, Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] How to fix frozen section?
It depends upon what marker you're staining for. We fix our renal biopsies for C4d in acetone for 10. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Lee Sent: Tuesday, November 06, 2012 1:41 PM To: histonet Subject: [Histonet] How to fix frozen section? Hello, I will receive some frozen tissue sections that are not fixed. I rarely do IHC on frozen sections. I searched some articles about fixation. Some use 50/50 of acetone/alcohol and some use only alcohol. Could you please recommend a fixation method for me? Thanks, Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histologist
Busy Derm Practice looking for an experienced Histologist to run Derm Path lab. Full or Part time. Must be able to perform all routine histology duties soley. Embedding, grossing, accession, coverslipping, microtonomy, record keeping, HE stain and special stains. Candidate needs to be familiar with OSHA and CLIA standards. Must be able to update and maintain all manuals. Mohs experience preferred. Pam Mathews, CDC Dermatology and Laser Center Office Manager 904-276-4500 Office 904-276-4160 Fax 904-945-6845 Cell ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] How to fix frozen section?
Amy, We use ice-cold acetone/ethanol (3:1) for 10'...works for all of our antigens so far. Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Lee Sent: Tuesday, November 06, 2012 2:41 PM To: histonet Subject: [Histonet] How to fix frozen section? Hello, I will receive some frozen tissue sections that are not fixed. I rarely do IHC on frozen sections. I searched some articles about fixation. Some use 50/50 of acetone/alcohol and some use only alcohol. Could you please recommend a fixation method for me? Thanks, Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Devasting news on 88305TC component--Where the $18 came from
Well in this article it does have differences, especially when talking about usage of technology,, but non the less it does expose our state of being within Anatomic Pathology,, ,, what are peoples thought on this Sent from my iPad On Nov 6, 2012, at 12:21 PM, Lester Raff MD lr...@uropartners.com wrote: Sent: Tuesday, November 06, 2012 12:35 PM To: 'histonet-boun...@lists.utsouthwestern.edu' Subject: RE: [Histonet] Devasting news on 88305TC component--Where the $18 came from The $18.00 figure came from an article published in Archives of Pathology and Lab Medicine: Pathology Economic Model Tool A Novel Approach to Workflow and Budget Cost Analysis in an Anatomic Pathology Laboratory David Muirhead, BSc; Patricia Aoun, MD, MPH; Michael Powell, MS, FASHP; Flemming Juncker, MBA; Jens Mollerup, MSc, PhD (Arch Pathol Lab Med. 2010;134:1164-1169) Thanks to Joe Plandowski of IOL for providing me with the reference. Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.492.0203 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webster, Thomas S. Sent: Monday, November 05, 2012 3:01 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Devasting news on 88305TC component I wish there was a way to put a positive spin on this but I can't think of any. We can only hope it kills off client billing somehow. Whomever the stakeholder was that told CMS a typical 88305 costs 18 bucks, I'd love to know how he/she came up with that number. It's insultingly low. http://www.acla.com/sites/default/files/ACLA%20comments%202012%20PFS%20p roposed%20rule%208-30-11_3.pdf I believe whoever it was had the goal to stop the proliferation of POLs. Wouldn't surprise me if they worked for a large national lab that had lost a lot of business to POLs. CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] How to fix frozen section?
I used acetone René J. From: Amy Lee amylee...@yahoo.com To: histonet histonet@lists.utsouthwestern.edu Sent: Tuesday, November 6, 2012 2:41 PM Subject: [Histonet] How to fix frozen section? Hello, I will receive some frozen tissue sections that are not fixed. I rarely do IHC on frozen sections. I searched some articles about fixation. Some use 50/50 of acetone/alcohol and some use only alcohol. Could you please recommend a fixation method for me? Thanks, Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] How to fix frozen section?
i use 1:1 Acetone : alcohol. On Nov 6, 2012, at 2:41 PM, Amy Lee wrote: Hello, I will receive some frozen tissue sections that are not fixed. I rarely do IHC on frozen sections. I searched some articles about fixation. Some use 50/50 of acetone/alcohol and some use only alcohol. Could you please recommend a fixation method for me? Thanks, Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best, Arjun ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD11b
Hello to Everyone!! :) Has anyone worked up CD11b in human tissue? We have tried one from Novus and one from Serotec with no staining or staining that was not specific. I would appreciate any help anyone could give! :) Thank you, Pat Pat Bell HT(ASCP) University of Colorado, Denver Medical Oncology; MS 8117 12801 E 17th Ave. RC1-S, L18-8402C Aurora, Co. 80045 303-724-6077 pat.b...@ucdenver.edumailto:pat.b...@ucdenver.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CD11b
Me too!!! C On 11/6/2012 3:44 PM, Bell, Pat wrote: Hello to Everyone!! :) Has anyone worked up CD11b in human tissue? We have tried one from Novus and one from Serotec with no staining or staining that was not specific. I would appreciate any help anyone could give! :) Thank you, Pat Pat Bell HT(ASCP) University of Colorado, Denver Medical Oncology; MS 8117 12801 E 17th Ave. RC1-S, L18-8402C Aurora, Co. 80045 303-724-6077 pat.b...@ucdenver.edumailto:pat.b...@ucdenver.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
