[Histonet] RE: Sonority Question
I would have to consider the tech who is working continuously as senior. That is the tech that I would rely on the most. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, February 12, 2013 4:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Senority Question I have 2 employees that are wondering about seniority... one HT has been on payroll for 5 years and has gone from FT to PRN and back to FT without any breaks. The other tech has been FT for 3 years. Which one is the senior in the lab? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Process and Hold?
Tom, another idea is to set your processor to do the weekend delay hold in 70% alcohol rather than in the formalin. Then the samples will go through the formalin as usual, and stop and hold in the 70% until weekend processing as scheduled begins. Then your samples will come off on Monday morning as usual, without extra fixation, and without requiring a weekend technician. The 70% alcohol does no harm at all to your samples. We do this routinely with other tissues for IHC in our lab with great results. Donna Donna J. Emge, HT-ASCP Mouse Histology and Phenotyping Core Manager Northwestern University Olson Building room 8-333 710 North Fairbanks Court Chicago, IL 60611 m...@northwestern.edu 312-503-2679 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Sonority Question
I have worked in places which do both. One lab considered hire date only while another calculated FT equivalent hours. Both have merit and it is usually set according to institutional policy. Mark Turner, Ph.D., HT(ASCP)QIHC Manager, Histology/IHC CSI Laboratories -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of susan.wal...@hcahealthcare.com Sent: Wednesday, February 13, 2013 3:15 AM To: amber.mcken...@gastrodocs.net; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Sonority Question I would have to consider the tech who is working continuously as senior. That is the tech that I would rely on the most. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, February 12, 2013 4:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Senority Question I have 2 employees that are wondering about seniority... one HT has been on payroll for 5 years and has gone from FT to PRN and back to FT without any breaks. The other tech has been FT for 3 years. Which one is the senior in the lab? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue processing for laser microdissection and RNA isolation?
Hello! May I ask for your recommendations for tissue processing methods for laser microdissection and subsequent RNA isolation? I can think of at least the following protocols, each with significant drawbacks (and questions): 1) Traditional FFPE sections: + easy handling + RNA is safe (but see below) + good morphology - RNA is fixed too, so yields are low and only small fragments retrieved Here, I'm pretty happy with Qiagen RNEasy FFPE kit - any other suggestions, maybe cheaper? 2) Traditional cryosections: + fairly good morphology + good yields, good quality RNA if everything goes well - RNA is easily destroyed - difficult to handle small samples without melting destroying RNA I haven't been very succesful with this option. 3) RNALater - cryosections: + RNA is safe + good RNA yields, good quality RNA - poor morphology - difficult to section We have problems making the tissues actually freeze for good sectioning - any tricks or tips here? 4) RNALater - paraffin sections? I haven't tried this yet, but should be doable through ethanol etc. Found some references claiming that the RNA quality is poor. 5) New commercial innovations like Qiagen/Prenalytix Paxgene Tissue kit, claiming to achieve both RNA stabilization and good morphology. I haven't tried any of these yet. Pricing is the obvious drawback. With best regards, Mikael Niku, PhD Department of Veterinary Biosciences University of Helsinki ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Process and hold?
We do this on a fairly routine basis with our GI biopsies and have found it to be most effective. Leaving them in the warm retort exposes the tissue to heat longer than normal and could cause some problems. On Feb 12, 2013, at 1:12 PM, Robert Schoonhoven robert_schoonho...@yahoo.com wrote: All you need to do is have them drain the chamber and place the cassettes on a nonabsorbent surface and allow them to cool to room temp. On Monday morning have the techs put the into the cassette storage on your embedding center and they should be ready to embed witin a few minutes. As the tissues are processed through paraffin they are protected and will not dry out. Robert Schoonhoven, HT/HTL (ASCP) From: Tom McNemar tmcne...@lmhealth.org To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Tuesday, February 12, 2013 1:56 PM Subject: [Histonet] Process and hold? Hello all, I was wondering about processing breast specimens (needle cores) on Fridays. We have asked our radiology department to try to avoid scheduling these breast biopsies on Fridays since we do not work weekends and are concerned about the extended time in formalin. I am thinking that we can run these specimens on a second processor over Friday night and have someone from the clinical lab come up and drain the paraffin. The tissues would then sit in a warm moist retort until Monday morning when they would be embedded and cut. I think the specimens would be fine. Processing would be complete at that point and they would hold in the unopened retort chamber. Our alternative is to have someone come in every Saturday morning just to remove and embed these specimens. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet /lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Process and Hold?
We do the same thing with good results! Michele -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Donna J Emge Sent: Wednesday, February 13, 2013 7:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Process and Hold? Tom, another idea is to set your processor to do the weekend delay hold in 70% alcohol rather than in the formalin. Then the samples will go through the formalin as usual, and stop and hold in the 70% until weekend processing as scheduled begins. Then your samples will come off on Monday morning as usual, without extra fixation, and without requiring a weekend technician. The 70% alcohol does no harm at all to your samples. We do this routinely with other tissues for IHC in our lab with great results. Donna Donna J. Emge, HT-ASCP Mouse Histology and Phenotyping Core Manager Northwestern University Olson Building room 8-333 710 North Fairbanks Court Chicago, IL 60611 m...@northwestern.edu 312-503-2679 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: grossing tools
The book exists - I've seen it. I think it was written by some guy who gives management seminars. But it mysteriously vanished - probably taken by aliens to de-evolutionize their home planet. That's my theory. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Tuesday, February 12, 2013 7:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: grossing tools Bruce Gapinsk HT (ASCP), Chief Histologist, Marin Medical Laboratories, PathGroup SF asks: Histonians, I'm sure we are not the only histology lab that deals with thick grossed specimens. Has anyone tried the new gross tools by Sakura? Or anything else that can cut ONE nickel thick. Tired of reprocessing. This grumpy old (74) pathologist would be happy to try them out, though I can cut freehand and almost never have a block reprocessed, but the skill eludes a lot of the young folks. Do you have a Web link for these tools? The problem would be getting a lab manager to spring for them. The Laboratory Manager's Handy-Dandy Guide to Making Life Hard for the Pathologist (I'm sure there is such a book, though I haven't actually seen one) specifies that grossing is a ritual requiring only a basic set of tools. One lab expected me to gross with an athame and chalice. I tried to explain to them that OSHA and the CAP would not permit me to gross skyclad. (You guys know I NEVER exaggerate.) Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] H.Pylori Control Blocks
Good Morning, We are in need of H.Pylori controls. Does anyone one know where we can get FFPE blocks positive for H.pylori? Thank you, Linda Musto ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: grossing tools
Jeanine H. Bartlett at the CDC notes: Sakura sells these. The grossing boards are items: 4800, 4801 and 4802. The grossing forks are 4803, 4804 and 4807. Ask your rep for a demo. http://sakura.ifuture.com/sakura.asp?ifut=1000336642970309899%2CTKWK%3E6%3D%3A5ST0 The prices of these tools are outrageous. The online catalog gives me no clue as to how they work. Bob Richmond Samurai Pathologist Maryville TN On Tue, Feb 12, 2013 at 8:01 PM, Bob Richmond rsrichm...@gmail.com wrote: Bruce Gapinsk HT (ASCP), Chief Histologist, Marin Medical Laboratories, PathGroup SF asks: Histonians, I'm sure we are not the only histology lab that deals with thick grossed specimens. Has anyone tried the new gross tools by Sakura? Or anything else that can cut ONE nickel thick. Tired of reprocessing. This grumpy old (74) pathologist would be happy to try them out, though I can cut freehand and almost never have a block reprocessed, but the skill eludes a lot of the young folks. Do you have a Web link for these tools? The problem would be getting a lab manager to spring for them. The Laboratory Manager's Handy-Dandy Guide to Making Life Hard for the Pathologist (I'm sure there is such a book, though I haven't actually seen one) specifies that grossing is a ritual requiring only a basic set of tools. One lab expected me to gross with an athame and chalice. I tried to explain to them that OSHA and the CAP would not permit me to gross skyclad. (You guys know I NEVER exaggerate.) Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] grossing tools
At 5:38 PM + 2/12/13, Bruce Gapinski wrote: I'm sure we are not the only histology lab that deals with thick grossed specimens. Has anyone tried the new gross tools by Sakura? Or anything else that can cut ONE nickel thick. A bane indeed, not only for histotechs, but for those doing the grossing. Our big problem is breast biopsies. The combination of fat and fibrous tissue makes it hard (impossible) to get consistent thicknesses manually. I have been trying to find some kind 'jig' that would hold inked breast biopsies of widely varying sizes that could guide the knife as the biopsy is bread-loafed to get consistent thicknesses. I'm not sure what words to look for or search for. OTOH, given our difficulty in getting paid for the tech component (we are an independent lab, long used to global billing), I dont want to spend a fortune for something. All advice is super-greatly appreciated. Bill -- __ Bill Blank, MD Heartland Lab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: grossing tools
That is why I suggested having a rep. bring them out to demonstrate. Sakura sells these. The grossing boards are items: 4800, 4801 and 4802. The grossing forks are 4803, 4804 and 4807. Ask your rep for a demo. Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, February 13, 2013 9:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: grossing tools Jeanine H. Bartlett at the CDC notes: Sakura sells these. The grossing boards are items: 4800, 4801 and 4802. The grossing forks are 4803, 4804 and 4807. Ask your rep for a demo. http://sakura.ifuture.com/sakura.asp?ifut=1000336642970309899%2CTKWK%3E6%3D%3A5ST0 The prices of these tools are outrageous. The online catalog gives me no clue as to how they work. Bob Richmond Samurai Pathologist Maryville TN On Tue, Feb 12, 2013 at 8:01 PM, Bob Richmond rsrichm...@gmail.com wrote: Bruce Gapinsk HT (ASCP), Chief Histologist, Marin Medical Laboratories, PathGroup SF asks: Histonians, I'm sure we are not the only histology lab that deals with thick grossed specimens. Has anyone tried the new gross tools by Sakura? Or anything else that can cut ONE nickel thick. Tired of reprocessing. This grumpy old (74) pathologist would be happy to try them out, though I can cut freehand and almost never have a block reprocessed, but the skill eludes a lot of the young folks. Do you have a Web link for these tools? The problem would be getting a lab manager to spring for them. The Laboratory Manager's Handy-Dandy Guide to Making Life Hard for the Pathologist (I'm sure there is such a book, though I haven't actually seen one) specifies that grossing is a ritual requiring only a basic set of tools. One lab expected me to gross with an athame and chalice. I tried to explain to them that OSHA and the CAP would not permit me to gross skyclad. (You guys know I NEVER exaggerate.) Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: grossing tools
We have a pair of the 3mm Cutmate forceps that we bought years ago for a particular pathologist but nobody uses them. If you go to the website below check out the Procut as well. http://www.milestonemed.com/histopathology/products.html Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Wednesday, February 13, 2013 9:57 AM To: Bob Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: grossing tools That is why I suggested having a rep. bring them out to demonstrate. Sakura sells these. The grossing boards are items: 4800, 4801 and 4802. The grossing forks are 4803, 4804 and 4807. Ask your rep for a demo. Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, February 13, 2013 9:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: grossing tools Jeanine H. Bartlett at the CDC notes: Sakura sells these. The grossing boards are items: 4800, 4801 and 4802. The grossing forks are 4803, 4804 and 4807. Ask your rep for a demo. http://sakura.ifuture.com/sakura.asp?ifut=1000336642970309899%2CTKWK%3E6%3D%3A5ST0 The prices of these tools are outrageous. The online catalog gives me no clue as to how they work. Bob Richmond Samurai Pathologist Maryville TN On Tue, Feb 12, 2013 at 8:01 PM, Bob Richmond rsrichm...@gmail.com wrote: Bruce Gapinsk HT (ASCP), Chief Histologist, Marin Medical Laboratories, PathGroup SF asks: Histonians, I'm sure we are not the only histology lab that deals with thick grossed specimens. Has anyone tried the new gross tools by Sakura? Or anything else that can cut ONE nickel thick. Tired of reprocessing. This grumpy old (74) pathologist would be happy to try them out, though I can cut freehand and almost never have a block reprocessed, but the skill eludes a lot of the young folks. Do you have a Web link for these tools? The problem would be getting a lab manager to spring for them. The Laboratory Manager's Handy-Dandy Guide to Making Life Hard for the Pathologist (I'm sure there is such a book, though I haven't actually seen one) specifies that grossing is a ritual requiring only a basic set of tools. One lab expected me to gross with an athame and chalice. I tried to explain to them that OSHA and the CAP would not permit me to gross skyclad. (You guys know I NEVER exaggerate.) Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Tissue processing for laser microdissection and RNA isolation?
If you fix adequately (no NBF) you can go with 1 René J. From: Mikael Niku mikael.n...@helsinki.fi To: histonet@lists.utsouthwestern.edu Sent: Wednesday, February 13, 2013 8:15 AM Subject: [Histonet] Tissue processing for laser microdissection and RNA isolation? Hello! May I ask for your recommendations for tissue processing methods for laser microdissection and subsequent RNA isolation? I can think of at least the following protocols, each with significant drawbacks (and questions): 1) Traditional FFPE sections: + easy handling + RNA is safe (but see below) + good morphology - RNA is fixed too, so yields are low and only small fragments retrieved Here, I'm pretty happy with Qiagen RNEasy FFPE kit - any other suggestions, maybe cheaper? 2) Traditional cryosections: + fairly good morphology + good yields, good quality RNA if everything goes well - RNA is easily destroyed - difficult to handle small samples without melting destroying RNA I haven't been very succesful with this option. 3) RNALater - cryosections: + RNA is safe + good RNA yields, good quality RNA - poor morphology - difficult to section We have problems making the tissues actually freeze for good sectioning - any tricks or tips here? 4) RNALater - paraffin sections? I haven't tried this yet, but should be doable through ethanol etc. Found some references claiming that the RNA quality is poor. 5) New commercial innovations like Qiagen/Prenalytix Paxgene Tissue kit, claiming to achieve both RNA stabilization and good morphology. I haven't tried any of these yet. Pricing is the obvious drawback. With best regards, Mikael Niku, PhD Department of Veterinary Biosciences University of Helsinki ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Process and hold?
I'm surprised that laboratories are not validating extended fixation times for their breast specimens rather than jumping through all these hoops. There are now several published articles demonstrating no reduction in immunoreactivity for ER, PR, and HER2 in breast specimens kept in formalin for 72 hours or longer (one of which is listed below). Please remember that the total time in formalin is only one of the pre-analytic variables that can affect immunoreactivity. Minimizing the cold ischemic time, making sure that the fresh tissue does not dry-out, and submitting THIN (2-3 mm) tissue sections for fixation and processing are equally, if not more important, than the total time that a specimen sits in formalin. Tong LC, Nelson N, Tsourigiannis J, et al.: The effect of prolonged fixation on the IHC evaluation of ER, PR, and HER2 expression in invasive breast cancer: A prospective study. Am J Surg Pathol 2011;35:545-552. A summation of their study; ... fixation for limited periods beyond 72 hours does not result in a reduction in assay sensitivity in the determination of ER, PR, or HER2 IHC status.. Obviously, each laboratory must do their own testing and validation, but it can be accomplished with team work (Pathologists, PAs, and Histotechnologists working together). Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax Tom McNemar tmcne...@lmhealth.org 2/12/2013 1:56 PM Hello all, I was wondering about processing breast specimens (needle cores) on Fridays. We have asked our radiology department to try to avoid scheduling these breast biopsies on Fridays since we do not work weekends and are concerned about the extended time in formalin. I am thinking that we can run these specimens on a second processor over Friday night and have someone from the clinical lab come up and drain the paraffin. The tissues would then sit in a warm moist retort until Monday morning when they would be embedded and cut. I think the specimens would be fine. Processing would be complete at that point and they would hold in the unopened retort chamber. Our alternative is to have someone come in every Saturday morning just to remove and embed these specimens. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Sonority Question
I wouldn't even touch that question and instead refer it to the all-knowing HR department. Too many legal issues involved in that one...and if there is a union involved they will have a say in it. Tim Morken UCSF Pathology -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, February 12, 2013 4:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Senority Question I have 2 employees that are wondering about seniority... one HT has been on payroll for 5 years and has gone from FT to PRN and back to FT without any breaks. The other tech has been FT for 3 years. Which one is the senior in the lab? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] grossing tools
I have found squeezing the tissue (for the block sections) between two cassettes held back to back gives the firmness needed to trim good thin sections. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bill B. Sent: Wednesday, February 13, 2013 9:58 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] grossing tools At 5:38 PM + 2/12/13, Bruce Gapinski wrote: I'm sure we are not the only histology lab that deals with thick grossed specimens. Has anyone tried the new gross tools by Sakura? Or anything else that can cut ONE nickel thick. A bane indeed, not only for histotechs, but for those doing the grossing. Our big problem is breast biopsies. The combination of fat and fibrous tissue makes it hard (impossible) to get consistent thicknesses manually. I have been trying to find some kind 'jig' that would hold inked breast biopsies of widely varying sizes that could guide the knife as the biopsy is bread-loafed to get consistent thicknesses. I'm not sure what words to look for or search for. OTOH, given our difficulty in getting paid for the tech component (we are an independent lab, long used to global billing), I dont want to spend a fortune for something. All advice is super-greatly appreciated. Bill -- __ Bill Blank, MD Heartland Lab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] grossing tools
How much squeezing? Tissues have certain elasticity and after the squeezing they will bounce back as a thicker slice. Free hand sectioning I think is always better. René J. From: Weems, Joyce K. joyce.we...@emoryhealthcare.org To: 'Bill B.' bill...@mindspring.com; histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Wednesday, February 13, 2013 11:09 AM Subject: RE: [Histonet] grossing tools I have found squeezing the tissue (for the block sections) between two cassettes held back to back gives the firmness needed to trim good thin sections. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org http://www.saintjosephsatlanta.org/ 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bill B. Sent: Wednesday, February 13, 2013 9:58 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] grossing tools At 5:38 PM + 2/12/13, Bruce Gapinski wrote: I'm sure we are not the only histology lab that deals with thick grossed specimens. Has anyone tried the new gross tools by Sakura? Or anything else that can cut ONE nickel thick. A bane indeed, not only for histotechs, but for those doing the grossing. Our big problem is breast biopsies. The combination of fat and fibrous tissue makes it hard (impossible) to get consistent thicknesses manually. I have been trying to find some kind 'jig' that would hold inked breast biopsies of widely varying sizes that could guide the knife as the biopsy is bread-loafed to get consistent thicknesses. I'm not sure what words to look for or search for. OTOH, given our difficulty in getting paid for the tech component (we are an independent lab, long used to global billing), I dont want to spend a fortune for something. All advice is super-greatly appreciated. Bill -- __ Bill Blank, MD Heartland Lab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Hello everyone! I have a question for all of the histo techs out there. It appears we are going to have to change some products and we are in need of some product recommendations. Currently we use EM400 paraffin for embedding and infiltration. Our department has tried to change paraffin before. The paraffins tested did not work well for us so I would like to know if anyone can tell me a comparable paraffin and from which vendor do you purchase? I may also need to change the blades that I use for microtomy. I prefer the Surgipath / Leica high profile thin disposable blades (Item 3802123) and I am looking for a similar blade. Thank you! Brendal Finlay, HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] (no subject)
Paraplast and Feather from Sakura René J. From: Brendal Finlay brendal.fin...@medicalcenterclinic.com To: histonet@lists.utsouthwestern.edu Sent: Wednesday, February 13, 2013 12:06 PM Subject: [Histonet] (no subject) Hello everyone! I have a question for all of the histo techs out there. It appears we are going to have to change some products and we are in need of some product recommendations. Currently we use EM400 paraffin for embedding and infiltration. Our department has tried to change paraffin before. The paraffins tested did not work well for us so I would like to know if anyone can tell me a comparable paraffin and from which vendor do you purchase? I may also need to change the blades that I use for microtomy. I prefer the Surgipath / Leica high profile thin disposable blades (Item 3802123) and I am looking for a similar blade. Thank you! Brendal Finlay, HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] 2013 Colorado Society of Histotechnology Meeting April 19th 20th
The 2013 Colorado Society of Histotechnology meeting will be held April 19th 20th at the La Quinta Inn, Fort Collins, CO. The program is now posted on the CSH website. For more information regarding the meeting, accommodations, online registration and payment, please visit http://www.coloradohisto.org/2013/meeting.htm. 2013 CSH Registration Packet: http://www.coloradohisto.org/2013/2013_CSH_Program.pdf Online registration is strongly encouraged. If you have to mail or fax your registration please print legibly (my eyes aren’t getting any younger) and provide a valid email address as receipts are no longer being mailed. If you are interested in attending the meeting please register sooner rather than later. As always, you have up until the day of the meeting to pay. Looking forward to seeing all of you at the meeting in April. Respectfully, Matt Lunetta BS HT(ASCP) Lead Histology Longmont United Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] grossing tools
Hi Joyce, I will try that thanks. It would keep them from slipping around too. This has come up since we have gone from 2-3 breast biopsies a month to 5 to 10 a week, and me being a solo pathologist, I've been imagining devices to speed things up and be more accurate. Regards, Bill Blank At 4:09 PM + 2/13/13, Weems, Joyce K. wrote: I have found squeezing the tissue (for the block sections) between two cassettes held back to back gives the firmness needed to trim good thin sections. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bill B. Sent: Wednesday, February 13, 2013 9:58 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] grossing tools At 5:38 PM + 2/12/13, Bruce Gapinski wrote: I'm sure we are not the only histology lab that deals with thick grossed specimens. Has anyone tried the new gross tools by Sakura? Or anything else that can cut ONE nickel thick. A bane indeed, not only for histotechs, but for those doing the grossing. Our big problem is breast biopsies. The combination of fat and fibrous tissue makes it hard (impossible) to get consistent thicknesses manually. I have been trying to find some kind 'jig' that would hold inked breast biopsies of widely varying sizes that could guide the knife as the biopsy is bread-loafed to get consistent thicknesses. I'm not sure what words to look for or search for. OTOH, given our difficulty in getting paid for the tech component (we are an independent lab, long used to global billing), I dont want to spend a fortune for something. All advice is super-greatly appreciated. Bill -- __ Bill Blank, MD Heartland Lab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Paraffin / Microtome Blades
Sorry about the lack of Subject... I knew I was forgetting something! ;) Brendal Finlay, HT (ASCP) -Original message- From: Rene J Buesa rjbu...@yahoo.com Date: Wed, 13 Feb 2013 12:09:08 -0600 To: Brendal Finlay brendal.fin...@medicalcenterclinic.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] (no subject) Paraplast and Feather from Sakura René J. From: Brendal Finlay To: histonet@lists.utsouthwestern.edu Sent: Wednesday, February 13, 2013 12:06 PM Subject: [Histonet] (no subject) Hello everyone! I have a question for all of the histo techs out there. It appears we are going to have to change some products and we are in need of some product recommendations. Currently we use EM400 paraffin for embedding andinfiltration. Our department has tried to change paraffin before. The paraffins tested did not work well for us so I would like to know if anyone can tell me a comparable paraffin and from which vendor do you purchase? I may also need to change the blades that I use for microtomy. I prefer the Surgipath / Leica high profile thin disposable blades (Item 3802123) and I am looking for a similar blade. Thank you! Brendal Finlay, HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Benchmarking information
We are working on a Lean/SixSigma project in our Histology Lab to decrease our TAT for pathology reports. Part of this process is to break down the total time and look at the different stages of the process to help identify where waste in the process is, such as: * Time from receipt in lab to delivery of slides to pathologists * Time special stains are ordered to delivery of slides to pathologists * Etc. Does anyone have any benchmarking information out there to share on this? Any help is appreciated. _ Sheila Tapper Anatomic Pathology Supervisor Essentia Health SMDC Laboratory Pathology 3W SMMC 407 East Third Street, Duluth, MN 55805 P: 218-786-5472 | F: 218-786-2369 sheila.tap...@essentiahealth.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: Re: [Histonet] grossing tools
I intend to make us some of these tools. It's a great idea. On 3:59, Rene J Buesa wrote: How much squeezing? Tissues have certain elasticity and after the squeezing they will bounce back as a thicker slice. Free hand sectioning I think is always better. René J. From: Weems, Joyce K.joyce.we...@emoryhealthcare.org To: 'Bill B.'bill...@mindspring.com; histonet@lists.utsouthwestern.eduhistonet@lists.utsouthwestern.edu Sent: Wednesday, February 13, 2013 11:09 AM Subject: RE: [Histonet] grossing tools I have found squeezing the tissue (for the block sections) between two cassettes held back to back gives the firmness needed to trim good thin sections. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org http://www.saintjosephsatlanta.org/ 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bill B. Sent: Wednesday, February 13, 2013 9:58 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] grossing tools At 5:38 PM + 2/12/13, Bruce Gapinski wrote: I'm sure we are not the only histology lab that deals with thick grossed specimens. Has anyone tried the new gross tools by Sakura? Or anything else that can cut ONE nickel thick. A bane indeed, not only for histotechs, but for those doing the grossing. Our big problem is breast biopsies. The combination of fat and fibrous tissue makes it hard (impossible) to get consistent thicknesses manually. I have been trying to find some kind 'jig' that would hold inked breast biopsies of widely varying sizes that could guide the knife as the biopsy is bread-loafed to get consistent thicknesses. I'm not sure what words to look for or search for. OTOH, given our difficulty in getting paid for the tech component (we are an independent lab, long used to global billing), I dont want to spend a fortune for something. All advice is super-greatly appreciated. Bill -- __ Bill Blank, MD Heartland Lab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: Re: [Histonet] grossing tools
I may be incorrect but I BEKLEIVE Dr. Azorides Morales at the Univ. of Miami designed the tools that Sakura manufactures. His is a huge teaching facility and I think they (pathologists, residents, etc.) all use them. (Could be wrong, but I seem to remember this a while back) http://uhealthsystem.com/doctors/profile/1109 Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of E. Wayne Johnson ??? Sent: Wednesday, February 13, 2013 1:05 PM To: Rene J Buesa Cc: histonet@lists.utsouthwestern.edu; 'Bill B.' Subject: Re: Re: [Histonet] grossing tools I intend to make us some of these tools. It's a great idea. On 3:59, Rene J Buesa wrote: How much squeezing? Tissues have certain elasticity and after the squeezing they will bounce back as a thicker slice. Free hand sectioning I think is always better. René J. From: Weems, Joyce K.joyce.we...@emoryhealthcare.org To: 'Bill B.'bill...@mindspring.com; histonet@lists.utsouthwestern.eduhistonet@lists.utsouthwestern.edu Sent: Wednesday, February 13, 2013 11:09 AM Subject: RE: [Histonet] grossing tools I have found squeezing the tissue (for the block sections) between two cassettes held back to back gives the firmness needed to trim good thin sections. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org http://www.saintjosephsatlanta.org/ 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bill B. Sent: Wednesday, February 13, 2013 9:58 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] grossing tools At 5:38 PM + 2/12/13, Bruce Gapinski wrote: I'm sure we are not the only histology lab that deals with thick grossed specimens. Has anyone tried the new gross tools by Sakura? Or anything else that can cut ONE nickel thick. A bane indeed, not only for histotechs, but for those doing the grossing. Our big problem is breast biopsies. The combination of fat and fibrous tissue makes it hard (impossible) to get consistent thicknesses manually. I have been trying to find some kind 'jig' that would hold inked breast biopsies of widely varying sizes that could guide the knife as the biopsy is bread-loafed to get consistent thicknesses. I'm not sure what words to look for or search for. OTOH, given our difficulty in getting paid for the tech component (we are an independent lab, long used to global billing), I dont want to spend a fortune for something. All advice is super-greatly appreciated. Bill -- __ Bill Blank, MD Heartland Lab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Immuno Bed Plastic Question
Hi histonetters! I have posted on this forum a while ago asking for opinions regardnig new types of plastics that work well with IHC. It was brought to my attention that Immuno-Bed (Polysciences) works rather well. I recently purchased the plastic, infiltrated/embedded well, and sectioned great, BUT, when I removed the excess GMA/rehydrated and labeled with my antibodies, NOTHING WORKED! Nothing showed up! Only DAPI!! The mechanism I used for removing GMA and rehydrating were as follows: 1.) 3 changes of 30 minutes each in 60 Celsius xylene. (Note: I recently let the slides sit in 60 Celsius xylene overnight too... but it did not work!!) 2.) 3 changes of 10 minutes each in 100% EtOH. 3.) 15 minutes in 95%, 75% 50% EtOH 4.) 5 minutes in water 5.) 3 changes of 5 minutes each, PBS. I continued to block and apply my antibodies as normal. (Antibodies are all brand new, not duds!) Since this plastic was formulated specifically for IHC, I am at a loss. If anyone has used this plastic before, any insight directed my way would be extremely helpful!! Thanks, Allyse Mazzarelli Histologist NEUROTECH USA INC. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Benchmarking information
Sheila: Please go to http://www.histosearch.com/rene.html to find data on these subjects. René J. From: Tapper, Sheila J. sheila.tap...@essentiahealth.org To: histonet@lists.utsouthwestern.edu Sent: Wednesday, February 13, 2013 12:45 PM Subject: [Histonet] Benchmarking information We are working on a Lean/SixSigma project in our Histology Lab to decrease our TAT for pathology reports. Part of this process is to break down the total time and look at the different stages of the process to help identify where waste in the process is, such as: * Time from receipt in lab to delivery of slides to pathologists * Time special stains are ordered to delivery of slides to pathologists * Etc. Does anyone have any benchmarking information out there to share on this? Any help is appreciated. _ Sheila Tapper Anatomic Pathology Supervisor Essentia Health SMDC Laboratory Pathology 3W SMMC 407 East Third Street, Duluth, MN 55805 P: 218-786-5472 | F: 218-786-2369 sheila.tap...@essentiahealth.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: Re: [Histonet] grossing tools
I have no idea how spell-check missed this but of course I meant to state, I BELIEVE Dr. -Original Message- From: Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Wednesday, February 13, 2013 1:10 PM To: E. Wayne Johnson 朱稳森; Rene J Buesa Cc: histonet@lists.utsouthwestern.edu; 'Bill B.' Subject: RE: Re: [Histonet] grossing tools I may be incorrect but I BEKLEIVE Dr. Azorides Morales at the Univ. of Miami designed the tools that Sakura manufactures. His is a huge teaching facility and I think they (pathologists, residents, etc.) all use them. (Could be wrong, but I seem to remember this a while back) http://uhealthsystem.com/doctors/profile/1109 Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of E. Wayne Johnson ??? Sent: Wednesday, February 13, 2013 1:05 PM To: Rene J Buesa Cc: histonet@lists.utsouthwestern.edu; 'Bill B.' Subject: Re: Re: [Histonet] grossing tools I intend to make us some of these tools. It's a great idea. On 3:59, Rene J Buesa wrote: How much squeezing? Tissues have certain elasticity and after the squeezing they will bounce back as a thicker slice. Free hand sectioning I think is always better. René J. From: Weems, Joyce K.joyce.we...@emoryhealthcare.org To: 'Bill B.'bill...@mindspring.com; histonet@lists.utsouthwestern.eduhistonet@lists.utsouthwestern.edu Sent: Wednesday, February 13, 2013 11:09 AM Subject: RE: [Histonet] grossing tools I have found squeezing the tissue (for the block sections) between two cassettes held back to back gives the firmness needed to trim good thin sections. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org http://www.saintjosephsatlanta.org/ 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bill B. Sent: Wednesday, February 13, 2013 9:58 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] grossing tools At 5:38 PM + 2/12/13, Bruce Gapinski wrote: I'm sure we are not the only histology lab that deals with thick grossed specimens. Has anyone tried the new gross tools by Sakura? Or anything else that can cut ONE nickel thick. A bane indeed, not only for histotechs, but for those doing the grossing. Our big problem is breast biopsies. The combination of fat and fibrous tissue makes it hard (impossible) to get consistent thicknesses manually. I have been trying to find some kind 'jig' that would hold inked breast biopsies of widely varying sizes that could guide the knife as the biopsy is bread-loafed to get consistent thicknesses. I'm not sure what words to look for or search for. OTOH, given our difficulty in getting paid for the tech component (we are an independent lab, long used to global billing), I dont want to spend a fortune for something. All advice is super-greatly appreciated. Bill -- __ Bill Blank, MD Heartland Lab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Immuno Bed Plastic Question
I think that you should bring this issue to the provider. For sure you will get a better answer than bringing this issue to HistoNet. René J. From: Allyse Mazzarelli allyse...@gmail.com To: histonet@lists.utsouthwestern.edu Sent: Wednesday, February 13, 2013 1:19 PM Subject: [Histonet] Immuno Bed Plastic Question Hi histonetters! I have posted on this forum a while ago asking for opinions regardnig new types of plastics that work well with IHC. It was brought to my attention that Immuno-Bed (Polysciences) works rather well. I recently purchased the plastic, infiltrated/embedded well, and sectioned great, BUT, when I removed the excess GMA/rehydrated and labeled with my antibodies, NOTHING WORKED! Nothing showed up! Only DAPI!! The mechanism I used for removing GMA and rehydrating were as follows: 1.) 3 changes of 30 minutes each in 60 Celsius xylene. (Note: I recently let the slides sit in 60 Celsius xylene overnight too... but it did not work!!) 2.) 3 changes of 10 minutes each in 100% EtOH. 3.) 15 minutes in 95%, 75% 50% EtOH 4.) 5 minutes in water 5.) 3 changes of 5 minutes each, PBS. I continued to block and apply my antibodies as normal. (Antibodies are all brand new, not duds!) Since this plastic was formulated specifically for IHC, I am at a loss. If anyone has used this plastic before, any insight directed my way would be extremely helpful!! Thanks, Allyse Mazzarelli Histologist NEUROTECH USA INC. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Immuno Bed Plastic Question
Well. If it is truly GMA then you will not be able to remove it. What you describe is one of several methods to remove MMA. You will also lose the occasional section on exposure to the higher ( 70% +) grades of alcohol. If it is GMA I'd suggest you place your slides directly in PBS and then start your IHC procedure. Robert Schoonhoven, HT/HTL (ASCP) From: Allyse Mazzarelli allyse...@gmail.com To: histonet@lists.utsouthwestern.edu Sent: Wednesday, February 13, 2013 1:19 PM Subject: [Histonet] Immuno Bed Plastic Question Hi histonetters! I have posted on this forum a while ago asking for opinions regardnig new types of plastics that work well with IHC. It was brought to my attention that Immuno-Bed (Polysciences) works rather well. I recently purchased the plastic, infiltrated/embedded well, and sectioned great, BUT, when I removed the excess GMA/rehydrated and labeled with my antibodies, NOTHING WORKED! Nothing showed up! Only DAPI!! The mechanism I used for removing GMA and rehydrating were as follows: 1.) 3 changes of 30 minutes each in 60 Celsius xylene. (Note: I recently let the slides sit in 60 Celsius xylene overnight too... but it did not work!!) 2.) 3 changes of 10 minutes each in 100% EtOH. 3.) 15 minutes in 95%, 75% 50% EtOH 4.) 5 minutes in water 5.) 3 changes of 5 minutes each, PBS. I continued to block and apply my antibodies as normal. (Antibodies are all brand new, not duds!) Since this plastic was formulated specifically for IHC, I am at a loss. If anyone has used this plastic before, any insight directed my way would be extremely helpful!! Thanks, Allyse Mazzarelli Histologist NEUROTECH USA INC. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] re: Immuno-Bed
Hi Allyse, Immuno-Bed is only recommended for lower molecular weight antibodies. Polyscience recommends using Osteo-Bed Bone Kit for the higher molecular weight ab's. Hope this helps. Pat Lenhart HT (ASCP) Univ. of Colorado Denver ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] Re: grossing tools
I watched the video at the milestone-website. Has anyone so much time during grossing? And what about the next three breast-specimens waiting in the row. Who cleans this tool between the specimens? And what about feeling the tissue? I think my pathologists would get mad before making the first cut... ;-) Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Tom McNemar Gesendet: Mittwoch, 13. Februar 2013 16:18 An: 'Bartlett, Jeanine (CDC/OID/NCEZID)'; Bob Richmond; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] Re: grossing tools We have a pair of the 3mm Cutmate forceps that we bought years ago for a particular pathologist but nobody uses them. If you go to the website below check out the Procut as well. http://www.milestonemed.com/histopathology/products.html Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Wednesday, February 13, 2013 9:57 AM To: Bob Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: grossing tools That is why I suggested having a rep. bring them out to demonstrate. Sakura sells these. The grossing boards are items: 4800, 4801 and 4802. The grossing forks are 4803, 4804 and 4807. Ask your rep for a demo. Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, February 13, 2013 9:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: grossing tools Jeanine H. Bartlett at the CDC notes: Sakura sells these. The grossing boards are items: 4800, 4801 and 4802. The grossing forks are 4803, 4804 and 4807. Ask your rep for a demo. http://sakura.ifuture.com/sakura.asp?ifut=1000336642970309899%2CTKWK%3E6%3D% 3A5ST0 The prices of these tools are outrageous. The online catalog gives me no clue as to how they work. Bob Richmond Samurai Pathologist Maryville TN On Tue, Feb 12, 2013 at 8:01 PM, Bob Richmond rsrichm...@gmail.com wrote: Bruce Gapinsk HT (ASCP), Chief Histologist, Marin Medical Laboratories, PathGroup SF asks: Histonians, I'm sure we are not the only histology lab that deals with thick grossed specimens. Has anyone tried the new gross tools by Sakura? Or anything else that can cut ONE nickel thick. Tired of reprocessing. This grumpy old (74) pathologist would be happy to try them out, though I can cut freehand and almost never have a block reprocessed, but the skill eludes a lot of the young folks. Do you have a Web link for these tools? The problem would be getting a lab manager to spring for them. The Laboratory Manager's Handy-Dandy Guide to Making Life Hard for the Pathologist (I'm sure there is such a book, though I haven't actually seen one) specifies that grossing is a ritual requiring only a basic set of tools. One lab expected me to gross with an athame and chalice. I tried to explain to them that OSHA and the CAP would not permit me to gross skyclad. (You guys know I NEVER exaggerate.) Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu
[Histonet] Histology Laboratory Supervisor position
TO APPLY: Please send resume to vsm...@pcgmolecular.com or fax to 678-928-9760. LOCATION: Dahlonega, Georgia 30533 SCHEDULE: Full Time Monday-Friday Day Shift Summary: Work involves repetitive laboratory tasks which require accuracy in the preparation of tissue blocks and slides, solvents, solutions, and compounds, and the routine maintenance and care of laboratory specimens, cultures, and equipment. Supervisory functions will include the process of hiring, training, competency assessments and performance appraisals. Additionally, will facilitate and develop continuing education programs, and provide backup for bench technicians in sectioning and staining as needed. Essential Job Duties: --Prepare tissues for processing, embedding, cutting, mounting and staining for microscopic examination. --Clean and maintain grossing room/area. --Assist with the daily grossing of specimens. --Perform appropriate staining of tissue. --Process body fluids for cytological examination. --Cover-slip slides appropriately. --Maintain and properly dispose of bio-hazardous waste. --Maintenance of policies, procedures and practices necessary to conduct the normal function of the histology laboratory. --Carry out routine duties and responsibilities with supervision. Make decisions and establishes work priorities on essential procedure-oriented operations. --Instruct laboratory personnel of functions that need to be maintained and performed. --Maintain maintenance records for laboratory equipment and ensures that all equipment is working. --Keep procedure manuals and other monthly/yearly records up to date according to CAP, CLIA and the State of Georgia in accordance with their standards and those of PCG Labs. --In charge of Histology inspections by CAP, CLIA and the State of Georgia. --Ensure that the daily functions of the laboratory are properly performed and improve the process if needed. Requirements: HT/HTL Certification Bachelor Degree in Biology or Science 5+ years of Experience as a Histotech LOCATION: Dahlonega, Georgia 30533 SCHEDULE: Full Time Monday-Friday Day Shift Summary: Work involves repetitive laboratory tasks which require accuracy in the preparation of tissue blocks and slides, solvents, solutions, and compounds, and the routine maintenance and care of laboratory specimens, cultures, and equipment. Supervisory functions will include the process of hiring, training, competency assessments and performance appraisals. Additionally, will facilitate and develop continuing education programs, and provide backup for bench technicians in sectioning and staining as needed. Essential Job Duties: --Prepare tissues for processing, embedding, cutting, mounting and staining for microscopic examination. --Clean and maintain grossing room/area. --Assist with the daily grossing of specimens. --Perform appropriate staining of tissue. --Process body fluids for cytological examination. --Cover-slip slides appropriately. --Maintain and properly dispose of bio-hazardous waste. --Maintenance of policies, procedures and practices necessary to conduct the normal function of the histology laboratory. --Carry out routine duties and responsibilities with supervision. Make decisions and establishes work priorities on essential procedure-oriented operations. --Instruct laboratory personnel of functions that need to be maintained and performed. --Maintain maintenance records for laboratory equipment and ensures that all equipment is working. --Keep procedure manuals and other monthly/yearly records up to date according to CAP, CLIA and the State of Georgia in accordance with their standards and those of PCG Labs. --In charge of Histology inspections by CAP, CLIA and the State of Georgia. --Ensure that the daily functions of the laboratory are properly performed and improve the process if needed. Requirements: HT/HTL Certification Bachelor Degree in Biology or Science 5+ years of Experience as a Histotech ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cd8 on mouse tissue
Is this still being done on frozen sections not aldehyde fixed or has
someone figured out an antibody or method of doing it on FFPE mouse tissue
yet???
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E. Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
mailto:rueggihcconsultin...@outlook.com rueggihcconsultin...@outlook.com
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WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Slide Mate printers
Hi, We recently installed Slide Mate printers and are experiencing poor print quality despite several onsite service calls. The vendor is attributing the problems to the slides we are using even though we are using Colorfrost slides from the manufacturer's List of Approved Slides. Has anybody successfully implemented these printers? If so, could you please share what slides you are using? Thanks in advance. Lori W Ontario, Canada ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
