Re: [Histonet] QIHC
Just to let everyone know - the Michigan Society of Histotechnologists have put together a Study Guide/Workbook, geared to help those studying for the QIHC. It is a workbook, where you have to look up the information in books, and write it in the booklet. There are no answers provided (it's not a multiple choice question book, it's writing out definitions and how procedures work). This workbook is tries to cover all the areas on the exam, as listed on the ASCP BOC QIHC exam content outline. It is to help people get organized in their studying. $20. For more information, go to the MSH webpage: www.mihisto.org Click on Education Click on Study Guides Print out the order form at the bottom. Disclosure statement: I am a member of MSH. I do not receive any money from the sale of the QIHC Study Guide. Any money made (after printing and mailing costs) goes to support MSH. Peggy A. Wenk, HTL(ASCP)SLS -Original Message- From: Renee H. Workman Sent: Friday, March 01, 2013 11:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] QIHC I will be taking the QIHC soon has anyone taken it recently. I lost my QIHC when I changed jobs and want to get it back. Renee H. Workman W: 804-527-1316 | F: 804-270-0917 rhwork...@uro.commailto:rhwork...@uro.com | www.uro.comhttp://www.uro.com/ Disclaimer: The email and files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the original recipient or the person responsible for the delivering the email to the intended recipient, be advised that you have received this email in error, and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you received this email in error, please delete it from your system without copying it, and notify the sender by reply email so that our address record can be corrected. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RESCUING UNDER DECALCIFIED BONE FROM PARAFFIN EMBEDDEDBLOCKS
Can you surface decal? After facing the block on the microtome, getting a full face, pour some decalcification fluid in the lid of a coplin jar. Place the block faced side down in the decal solution, for about 30 minutes. Rinse the acid off the block with some cool water (don't want acid dripping on the microtome blade holder and blade). Line the block up exactly on the microtome to the knife. The first 2-4 sections will be decalcified enough for you to cut. (That's why the block has to be lined up exactly - can't waste rough trimming off the 10-20 um of decalcified tissue.) Peggy A. Wenk, HTL(ASCP)SLS Beaumont Health System Royal Oak, MI 48073 The opinions expressed are mine, and do not reflect on my place of employment. -Original Message- From: Fiona J Morrow Sent: Thursday, February 28, 2013 8:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RESCUING UNDER DECALCIFIED BONE FROM PARAFFIN EMBEDDEDBLOCKS Hi Has anyone ever tried to reprocess bone tissue that has been under decalcified and processed through to wax? Thanks Fi M Fiona Morrow Dept. of Infection and Immunity KFloor, Room K118 Medical School University of Sheffield Beech Hill Road Sheffield S10 2RX 0114 271 2102 f.mor...@sheffield.ac.uk ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Unencased Ameoba Stain
Getting back a little late (been out of state). If this is Acanthamoeba, then we do a PASH and/or a GMS. These are cysts, and show up nicely with both. The cornea can be PAS positive also, so the Acanthamoeba are a darker pink against a lighter pink, while the GMS is gray/black against the green background. It looks a lot like pneumocystis with a GMS, but without the bull's eye, and about 2-3 times larger. But it's the cyst wall that is staining with the PAS and the GMS, as the cyst has a lot of glycogen. But you are asking for unencased amoeba. So that sounds like no cyst. Are you looking for just the trophozoites? I would suggest one of the Giemsa stains, or maybe a Brown and Hopps. A cresyl echt violet (actually cresyl violet acetate, but everyone still calls it by it's old name), would probably also work - I read about it in the NSH Journal of Histotechnology about 20 years ago for the trophozoites of pneumocystis, but I've never tried the stain. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Health System Royal Oak, MI 48073 The opinions expressed are mine, and do not represent BHS. -Original Message- From: Joseph Brooks Sent: Wednesday, February 27, 2013 11:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Unencased Ameoba Stain Hello All, One of our Neuropath docs is inquiring about a special stain for unencased ameobas in cornea biopsies. I did a search and Gridley's Method was the best option that appreaded. Is there someone that could either verify this stain will work on this organism or let me know what you stain you are using? Thanks in advance. Matt Brooks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: Re: [Histonet] teeth sectioning
Jack, That sounds really awesome. I did some work with the teeth of sows (female pigs) from specimens collected at slaughter. Those are very difficult to decalcify, and when finished, are likely to have no nuclear detail remaining. Interested to hear what you learn Wayne Johnson Beijing Enable Ag Consulting Yuanmingyuan West Road Meiyuan Com, On 3:59, Jack Ratliff wrote: Mes, This is a very good question and I look forward to answers from individuals that have accomplished this with PMMA and a rotary microtome with tungsten-carbide knives. If you are talking about an undecalcified specimen embedded in PMMA, then I would imagine that the age of the rat could affect the ability to achieve an adequate infiltration of the resin. Again, I look forward to what others have to say about their success by the method you have outlined. On the other hand, I know you can achieve the micron thickness you desire if you were to use a non-contact femtosecond laser! The machine I am talking about is basically a laser microtome manufactured by Rowiak in Germany and it is officially called the TissueSurgeon. In fact, Dr. Heiko Richter from Germany has accomplished what you ask with human teeth, revealing the full anatomy of the tooth and even with ameloblasts on the enamel surface! I would be interested to hear more about your project. I will be traveling to Germany one week from today to work with this laser microtome until the end of the month so I could arrange to have laser cut sections made for you if you are interested and unable to make your cuts using PMMA and a rotary microtome. If you would like more information, please feel free to contact me by email reply. Best Regards, Jack Jack L Ratliff Owner/Histologist, Ratliff Histology Consultants, LLC Chairman, Hard Tissue Committee - National Society for Histotechnology On Mar 1, 2013, at 7:03 PM, mesruh turkekulturke...@gmail.com wrote: Dear Histonetters, I have one more question. Is it possible to obtain 5-10um thick sections of PMMA embedded teeth using regular Leica paraffin microtome (RM2265) equipped with disposable tungsten carbide blade? Thanks, Mes On Fri, Mar 1, 2013 at 7:40 PM, mesruh turkekulturke...@gmail.com wrote: Dear Histonetters, I am studying bone and teeth growth in rat maxilla. I will inject calcein green and would like to fix, embed and sections the rat maxilla. Any suggestions for the best method to fix, embed and section the samples for fluorescnet microscopy? Thank you very much! Mes HTL (ASCP) Memorial Sloan-Kettering Cancer Center On Fri, Mar 1, 2013 at 1:01 PM, histonet-requ...@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. FNA Clia Guidelines (PicheGrocki, Jessica) 2. RE: FNA Clia Guidelines (Horn, Hazel V) 3. GSH Symposium April 12-14 (Zimmerman, Billie) 4. FSH abstracts deadline (Jerry Santiago, MSEd, HTL (ASCP) QIHC) 5. (no subject) (Vikrant Piprode) 6. The GSH 4oth Anniversary Meeting (David Kemler) 7. QIHC (Renee H. Workman) -- Message: 1 Date: Thu, 28 Feb 2013 20:26:30 + From: PicheGrocki, Jessicajpiche-gro...@wtbyhosp.org Subject: [Histonet] FNA Clia Guidelines To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 631955447a364b45b9458d2905635110655d2...@win08-mbx-01.wtbyhosp.org Content-Type: text/plain; charset=s-ascii Hi All, Quick questionwhat are the Clia requirements for Fine needle aspirate procedures? Is it considered high complexity testing? And who prepares the slides when the needle is handed off? Thank you, Jessica Piche, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital -- Message: 2 Date: Thu, 28
[Histonet] pH and temperature
Hello! Can someone explain why pH is temperature dependent? Really, I'm just worried about pHing 4% paraformaldehyde at about 50 degrees Celsius. If I wait until it cools down to room temp, the para usually precipitates--I assume because the pH is off. I know I could measure the pH at room temperature, but I'm not making para anytime soon, and I just thought of this. By the way, the pH we are aiming for is 7.2 to 7.4 Emily By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward. -Chuck Palahniuk, Haunted ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] KY HT Poisitons
Please no Recruiter calls Great opportunity for Histotechnician's in Crestview Hills, KY ! Tri-State Gastroenterology Associates is a multi-physician practice located in Northern Kentucky. Its mission is To provide compassionate, high quality, cost-effective care to pateints with gastrointestinal problems Looking for 2 Full Time HT/HTL's ( 32 hours a week is full time employement at this practice ) * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens * Supervisor experience preferred * HT/HTL ASCP Certified * Experience with CLIA and CAP * Experience writing and maintaining policies and procedures * Prior laboratory start up experience is preferred * Ability to work independently Duties include: * Grossing * Embedding * Microtomy * Staining; routine and special stains only * Maintain supply orders and laboratory budget * Ability to be flexible and take on additional duties' as needed * Ability to work independently * Maintenance of laboratory for inspections * Maintenance of quality records Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mh...@miracals.commailto:mh...@miracals.com Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mh...@miracals.commailto:mh...@miracals.commailto:mh...@miracals.com%3cmailto:mh...@miracals.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] standards for embedding
I know this topic has been beaten to death in the past and do realize it is dependent on tissue orientation, quantity of pieces in a block, and experience. There are guidelines suggested by the College of Med Lab of Ontario in a document Oct 2008 that indicates the expected minimum daily range is Specimens with no orientation 16-38 seconds per specimen Specimens with orientation21 to 52 seconds peer specimen Mixed cassettes 60-70 per hour I would like to know if this is monitored and what do you if your techs are unable to reach these recommendations, even after several years of experience. I realize these are minimum suggestions and should be achievable but what recommendations are suggested if a tech with over 10 years experience still averages close to 2 minutes a block. On average we may have 120-140 blocks a day and it takes 3.5-4 hours to embed. I have had ergonomic assessments done. They seem to maintain a constant level and will not strive for improvement. Any suggestions? Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] standards for embedding
Ontario standards are above national and international averages and would be difficult to duplicate for the majority of labs. Your averages are more in accordance with the averages I mention. Please go to http://www.histosearch.com/rene.html to compare with the results of a survey I published. René J. From: Diana McCaig dmcc...@ckha.on.ca To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Sent: Monday, March 4, 2013 11:07 AM Subject: [Histonet] standards for embedding I know this topic has been beaten to death in the past and do realize it is dependent on tissue orientation, quantity of pieces in a block, and experience. There are guidelines suggested by the College of Med Lab of Ontario in a document Oct 2008 that indicates the expected minimum daily range is Specimens with no orientation 16-38 seconds per specimen Specimens with orientation 21 to 52 seconds peer specimen Mixed cassettes 60-70 per hour I would like to know if this is monitored and what do you if your techs are unable to reach these recommendations, even after several years of experience. I realize these are minimum suggestions and should be achievable but what recommendations are suggested if a tech with over 10 years experience still averages close to 2 minutes a block. On average we may have 120-140 blocks a day and it takes 3.5-4 hours to embed. I have had ergonomic assessments done. They seem to maintain a constant level and will not strive for improvement. Any suggestions? Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RELIA Solutions HOT JOB ALERT and a link to my blog post Essential Tips for Working with a Recruiter 3/4/2013
Hi Histonetters!! I hope your week is off to a great start!! I know mine is!! I have a couple of new opportunities that I want to share. Also I recently started a blog called Dream Job Diva here is the link to one of my posts if you think you might like to check it out. This has been without a doubt my most popular post which is why I chose it to share. I have even had other recruiting firms praise this post. - Enjoy. Here is the link: http://reliasolutionspambarker.wordpress.com/2012/07/28/esssential-tips-for- working-with-a-recruiter/ Here are the hot jobs I want to share with you! Histology Tech - East Texas - exciting opportunity to work in dermpath AND work with one of the top Mohs techs in the country! Histo Tech - Syracuse, NY - NYS license required Histotechnician - East of Columbus, OH IHC Specialist - Charlotte, NC Grossing Histotech - Charlotte, NC All of my clients offer excellent compensation, benefits and relocation assistance. These are full time permanent positions with stable secure labs. My clients are eager to interview and hire for these positions! If any of these opportunities are the right one for you RELIA can make it happen. If you do take the time to read my blog post I would love feedback. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: rel...@earthlink.net www.facebook.com http://www.facebook.com/PamBarkerRELIA /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Jekyll Island, GA
Come to Jekyll Island for the sunrise, sunsets, and CEU's!! Earn up to 15 CEU's with incredible speakers and a chance to network with your peers for the low all inclusive rate of $135. GSH is packing a punch for $135. GSH has planned an educational 40th anniversary celebration with fun, food, and time to visit the vendors. We have filled our block of rooms, but the symposium rate has been extended and is still available at Oceanside Inns and Suites. Also, if you love to ride bikes, bring them to the beach. I was impressed with the number of bike trails on the island. Go to our website and join the celebration. www.histosearch.com/gsh/http://www.histosearch.com/gsh/ If you have any questions or concerns please contact one of our officers. Hope to see you there next month! Wanda K. Simons GSH President WS/bz Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to bzimm...@gru.edu. Please update your address book to reflect this change. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: teeth sectioning
This might or might not be helpful, but there was an article written by Janet Maas, Processing the Complete Canine Tooth. It is for paraffin sectioning. JOH, Number 3, Sept 2002, pp. 137-140(4) Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: pH and temperature
Well, I found this on the site below: To make an accurate pH measurement you need to use temperature compensation. Exception is if you measure a pH of about 7. Which makes me feel better. Every other solution I pH is made at room temperature. I don't know why our para precipitates out--it might be because sometimes the water doesn't reach a high enough temperature before it's added, or the para solution cools down too much before the NaOH is added. It hasn't done it for a while though. Emily By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward. -Chuck Palahniuk, Haunted On Mon, Mar 4, 2013 at 1:28 PM, Tamara Howard thow...@unm.edu wrote: It is pretty basic chemistry...Even has a website! http://www.all-about-ph.com/ph-versus-temperature.html ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ventana HPV High/Low Replacements?
Hi Jennifer, I'd recommend looking at this study and comments on biomarkers in HPV: The Lower Anogenital Squamous Terminology Standardization Project for HPV-Associated Lesions: Background and Consensus Recommendations from the College of American Pathologists and the American Society for Colposcopy and Cervical Pathology Arch Pathol Lab Med-Vol 136, October 2012 The CAP-ASCCP LAST Project--Darragh et al Regards, Mike Michael Kent, MS, PhD www.dermpathlab.com Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. Thank you. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer Masuzumi Sent: Friday, March 01, 2013 2:09 PM To: Histonet Subject: [Histonet] Ventana HPV High/Low Replacements? Hello everyone, My lab is interested in starting HPV High and Low Risk ISH testing, but it seems like we just missed the boat on it. We have Benchmark XTs, but Ventana discontinued their HPV probes sometime around last December, and they don't have an alternative at this time. After talking with nearby labs that are currently using stockpiles of Ventana probes, they told me the following courses of action they were planning on using when their stock runs out: -One lab is thinking of doing HPV by PCR -Another is thinking of switching to Dako probes After discussing the possibility of Dako probes with Ventana and Dako, neither could recommend using them together because each side was unfamiliar with the other's reagents or system, though both said it might be possible. Is anybody in a similar situation, looking for a replacement for the Ventana HPV High/Low Risk probes? Any insight on possibilities to pursue would be much appreciated! Sincerely, Jennifer Masuzumi Sterling Pathology Seal Beach, CA jennifer.masuz...@sterlingpath.com 562-799-8900 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] QC Slide retention
How long do you folks retain you Daily QC slides? Is it the same as the diagnostic slides? I know the correct length of time for diagnostic slides in my particular area, but I wasn't sure if the same timeline applies to the actual daily HE or PAP QC slides. Is the retention of the actual documentation that I have of the quality of these stains each day sufficient? I looked thru the archives, but could only find the previous discussions on Diagnostic slides. Thanks Histonetters!! Rebecca Riesen, Histology Supervisor, PRMC, Naples, FL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sponges and Processing
If there is anyone out there that uses the blue sponges for biopsy specimens, would you please contact me offline? I would like to know what your processing times are. Thanks! Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: QC Slide retention
We keep ours for 2 years- kind of like those lovely QC sheets for CAP. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Riesen, Rebecca Sent: Monday, March 04, 2013 2:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] QC Slide retention How long do you folks retain you Daily QC slides? Is it the same as the diagnostic slides? I know the correct length of time for diagnostic slides in my particular area, but I wasn't sure if the same timeline applies to the actual daily HE or PAP QC slides. Is the retention of the actual documentation that I have of the quality of these stains each day sufficient? I looked thru the archives, but could only find the previous discussions on Diagnostic slides. Thanks Histonetters!! Rebecca Riesen, Histology Supervisor, PRMC, Naples, FL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: QC Slide retention
We do the same. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Monday, March 04, 2013 3:46 PM To: Riesen, Rebecca; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: QC Slide retention We keep ours for 2 years- kind of like those lovely QC sheets for CAP. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Riesen, Rebecca Sent: Monday, March 04, 2013 2:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] QC Slide retention How long do you folks retain you Daily QC slides? Is it the same as the diagnostic slides? I know the correct length of time for diagnostic slides in my particular area, but I wasn't sure if the same timeline applies to the actual daily HE or PAP QC slides. Is the retention of the actual documentation that I have of the quality of these stains each day sufficient? I looked thru the archives, but could only find the previous discussions on Diagnostic slides. Thanks Histonetters!! Rebecca Riesen, Histology Supervisor, PRMC, Naples, FL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Per Diem opportunity near Port Chester, NY
Allied Search Partners is currently working with a large organization with leading edge technologies to find a qualified Histology professional. Location: White Plains/Port Chester, NY area Position title: Histotechnician or Histotechnologist Schedule: Sunday 5am-1:30pm Email bran...@alliesearchpartners.com for a full job description. NY lab license is required. Brannon Owens Recruitment Manager Allied Search Partners ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Antibody IDH1
We use Dianova's antibody at a 1:100 dilution on the Ultra. CC1 cell conditioning for 40 minutes, antibody for 32 minutes - no heat. We use Optiview detection system. No amp. Make sure you are using the correct type of brain tissue for a control - most oligodendrogliomas should be positive, altho there are a few with other mutations. Remember that this antibody only picks up the R132H point mutation. This occurs in about 70% of oligos so it is possible that you happened to pick one that was positive for one of the other mutations. Perhaps try a few different patient tissues. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Zimmerman, Billie Sent: Friday, February 22, 2013 4:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Antibody IDH1 Does anyone perform this antibody?? We can't get it to work on the Ventana Ultra. PhenoPath in Seattle performs it on the Dako autostainer using a small size polymer detection kit. I would appreciate any input. Thanks, Billie Zimmerman Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to bzimm...@gru.edu. Please update your address book to reflect this change. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] SOP numbering for whole lab
Help? What kind of formula do you use when numbering and coding your SOPs for the lab? Is there an easy way to keep them sorted and in line with all the regulatory bodies to help on inspection days? Any suggestions welcome--we're starting from scratch. Cheryl ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Back processing Histogel
Has anyone back processed something that was both Histogel and FFPE embedded? If so, any hints or problems? Andrea ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Paraffin block archival storage
Does anyone know if there is a CAP or board of health regulation on storing archival paraffin blocks in a walk in morgue cooler. Currently we are storing our archival paraffin blocks in the morgue cooler for the ten year retention CAP regulation. The blocks are at a controlled temperature where the integrity of the blocks are not compromised. The blocks are stored in their block filing cabinet in one part of the cooler. Is there a problem with having the blocks stored in this area. Lastly the cooler is under lock and key and the key has to be signed out as well. This also provide a security aspect for the blocks and monitors who enters in and out of this secured area. Thanks for your help. Clarence Owens, HT (ASCP) Lawrence General Hospital Lawrence, MA 01842 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
