[Histonet] FFPE for Immunohistochemistry.
Pretreatments are used to recover bonded antigen sites owing to formalin linkage. What is the optimum or maximum fixation time for tissues that may require Immunohistochemistry staining? IB ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Formalin Neutralizer
We are looking into the option of neutralizing our formalin waste instead of having it to be picked up. If you use Formalin Neutralizer, do you have any pro/con about this product? Thank you very much. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas AM University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] compression problems
Hi all, Has anyone experienced a problem with Paraplast Xtra over the last 3-4 months? We are going crazy with compression problems and have tried all the usual solutions (tightening all parts of microtome, changing angles, changing blades, servicing microtome) and the problem persists. This involves all 4 of our microtomes to varying degrees and all types of tissue. The only commonality is the paraffin. The tissue itself does not compress, but the paraffin around it looks like horizontal stripes. We use Paraplast Xtra for both infiltrating and embedding. Any suggestions? Denise Crowley Hope Babette Tang Histology Facility Koch Center for Integrative Cancer Research Massachusetts Institute of Technology 500 Main St. 76-182 Cambridge MA 02139 617-258-8183 dencr...@mit.edumailto:dencr...@mit.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Formalin Neutralizer
We neutralize ours and have no problems with it. I am not sure how much you use, so I will say it is easiest in smaller batches as you do have to shake it up to make sure it dissolves. We purchase ours from BBC Biochemical for a very reasonable price and then test before dumping. We have doing this for four years with no issues. Thanks, Cristi Sent from my iPhone On Apr 11, 2013, at 6:04 AM, Bustamante, Lin lbustama...@cvm.tamu.edu wrote: We are looking into the option of neutralizing our formalin waste instead of having it to be picked up. If you use Formalin Neutralizer, do you have any pro/con about this product? Thank you very much. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas AM University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Talk to me about shelf life and outdates
We have stains from at least twenty years ago, judging by their labels--they have no date on them! I always wondered how long neutral red lasts, but apparently, it lasts a long, long time. We are not a clincial lab though, we do research. Emily By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward. -Chuck Palahniuk, Haunted On Wed, Apr 10, 2013 at 6:51 PM, Elizabeth Chlipala l...@premierlab.comwrote: Cheryl We are not a clinical lab, but we are GLP compliant. This is how we deal with dry chemicals and stains. A lot of times they do not come with an expiration date. If no expiration date/retest date is listed we give them an expiration date/retest date of 5 years post receipt. After that date we place them in a special area that is marked - Past Retest Date - Do not use until retested. We have a reagent control SOP that explains all of this. I'm not familiar with the Good until used designation. For retest we just run a special stain that contains a solution prepared from the chemical and if it stains appropriately we give that chemical a new expiration/retest date and just make sure we document the process appropriately. We just initiated this SOP recently so have not yet had to perform any retests. We update our chemical inventory as new chemicals are ordered, but once a year we review and check all our chemicals for inventory and that we have updated MSDS's available. I can send a pdf of our reagent control SOP if you would like it. If you are striving to be a GLP compliant lab and are heading to NSH convention this year I'll be giving a workshop on GLP compliance in the histology lab with our Facility Quality Assurance Manager. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 l...@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Wednesday, April 10, 2013 4:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Talk to me about shelf life and outdates We're prepping for an inspection and I have a small amount of older powdered dyes and substances we can't get any more.They're dated 'Good until used'. Would someone elaborate on how we came to have outdates on things that never had them before, and how those outdates are determined? Is there a procedure or policy you might share so we can keep our old but good items in service until we run out rather than in 12 months (EEEK!!!) Many thanks! Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT Tech at a time. 281.852.9457 Office 800.756.3309 Phone Fax ad...@fullstaff.org Sign up for the FREE newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to apn...@fullstaff.org. Please include your name and specialty in the body of the email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: compression problems
Denise We use paraplast and paraplast extra and have not experienced any problems. We just got a new lot of paraplast extra in and so far everything seems ok. I would check to see if it's a particular lot number that you are using. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 l...@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Denise G Crowley Sent: Thursday, April 11, 2013 7:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] compression problems Hi all, Has anyone experienced a problem with Paraplast Xtra over the last 3-4 months? We are going crazy with compression problems and have tried all the usual solutions (tightening all parts of microtome, changing angles, changing blades, servicing microtome) and the problem persists. This involves all 4 of our microtomes to varying degrees and all types of tissue. The only commonality is the paraffin. The tissue itself does not compress, but the paraffin around it looks like horizontal stripes. We use Paraplast Xtra for both infiltrating and embedding. Any suggestions? Denise Crowley Hope Babette Tang Histology Facility Koch Center for Integrative Cancer Research Massachusetts Institute of Technology 500 Main St. 76-182 Cambridge MA 02139 617-258-8183 dencr...@mit.edumailto:dencr...@mit.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] FFPE for Immunohistochemistry.
It depends on the antigen. I know that sounds like a cop out but it is true. Review the literature covering the antigen you are looking for to see what has worked for others Geoff On 4/11/2013 6:19 AM, Ian R Bernard wrote: Pretreatments are used to recover bonded antigen sites owing to formalin linkage. What is the optimum or maximum fixation time for tissues that may require Immunohistochemistry staining? IB ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Anti-human nuclear antibody
Forgot to mention this is on FFPE tissue From: Elizabeth Cameron Sent: Thursday, April 11, 2013 11:21 AM To: histonet@lists.utsouthwestern.edu Subject: Anti-human nuclear antibody Just wondering if anyone has had any luck with Millipore anti-nuclear antibody MAB1281, and if so, what was your protocol? We are trying to detect human cells in mice. Thank you. Elizabeth M. Cameron, HT, QIHC (ASCP) Histology Supervisor The Jackson Laboratory Bar Harbor, Maine The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: FFPE for Immunohistochemistry.
Generally under- fixation in formalin is more of a problem than longer fixation. The reason is first that less fixation does not preserve the tissue and antigens as well (more extraction of proteins in the processing steps), and second, antigen retrieval of any type is more damaging to under-fixed tissue. Studies have shown that 6 hours is a minimum to achieve formalin cross-linking of peptides and inactivate enzymes. After that time period antigen retrieval will be reproducible. Longer fixation is ok. Many studies have shown good antigen retrieval for the vast majority of antigens even after months of fixation when buffer-based heat antigen retrieval is used (as opposed to enzyme digestion). An optimum time is probably in the neighborhood of 16 to 24 hours. But most labs don't do that (clinical labs) and usually 6-12 hours fixation is used. Her2 and ER/PR guidelines specify minimum 6 hours fixation for bx cores and trimmed tissue. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ian R Bernard Sent: Thursday, April 11, 2013 3:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FFPE for Immunohistochemistry. Pretreatments are used to recover bonded antigen sites owing to formalin linkage. What is the optimum or maximum fixation time for tissues that may require Immunohistochemistry staining? IB ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Anti-human nuclear antibody
Just wondering if anyone has had any luck with Millipore anti-nuclear antibody MAB1281, and if so, what was your protocol? We are trying to detect human cells in mice. Thank you. Elizabeth M. Cameron, HT, QIHC (ASCP) Histology Supervisor The Jackson Laboratory Bar Harbor, Maine The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Society of Ohio Symposium/Convention April 26-27
Hi Histonetters!! I hope everyone is having a great day. I was asked by a friend to forward this information to you about the Histology Society of Ohio's Symposium/Convention which will be held April 26-27 2013 at the Holiday Inn Cleveland- West For more info and the program schedule please go to www.ohiohistology.org Histology Society of Ohio Symposium/Convention April 26-27, 2013 Holiday Inn Cleveland-West Westlake, OH www.ohiohistology.org Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: rel...@earthlink.net www.facebook.com http://www.facebook.com/PamBarkerRELIA /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Anti-human nuclear antibody
Elizabeth, We are able to detect human cells in mice organs using MAB1273 anti-mitochondria antibody (1:50) from Millipore on FFPE sections after standard HIER retrieval. As a detection reagent we use MaxFluor 594 Mouse on Mouse IF detection kit (MaxVision, cat.No MF03), Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: agleiber...@cbiolabs.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth Cameron Sent: Thursday, April 11, 2013 11:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Anti-human nuclear antibody Just wondering if anyone has had any luck with Millipore anti-nuclear antibody MAB1281, and if so, what was your protocol? We are trying to detect human cells in mice. Thank you. Elizabeth M. Cameron, HT, QIHC (ASCP) Histology Supervisor The Jackson Laboratory Bar Harbor, Maine The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Whole slide scanners.
I have worked with whole slide scanners and digital image analysis since 1998. We currently own scanners from 3 different vendors. Also, I have participated in whole slide scanner marketing research, sitting at a discussion table with a dozen people from different hospitals. My experience has been that most departments purchase scanners without a clear need. For example, some want to scan and archive slides, to make them available for review. That makes some sense in theory. However, in every institution I've talked to, they end up paying for the scanner, a tech to do the work, the service contract and the server space to store the images, which can be huge, up to 20 *giga*bytes per *slide... then find that it is rare for anyone to ever actually look at the scanned images. Most pathologists find it is more expedient to simply pull the glass slides out of the archive. In New York, we are required by law to archive glass slides for at least 10 years anyway. So, my advice, for what it's worth, is not to purchase any whole slide scanner unless you have a clear and well defined need (we have 3 giant systems, rarely used, taking up space and gathering dust). For example, if you need to store images from consultation slides before returning them to another institution, why not just snap a few photomicrographs? Being able to view a couple of small, representative pictures is better than having to wade through an entire scanned slide. --- Scott Ely, MD MPH Associate Director, Hematopathology Fellowship Program Section of Hematopathology Department of Pathology Weill Medical College of Cornell University New York Presbyterian Hospital Room: Starr 715 525 E. 68th Street New York, NY 10065 PH: 212-746-2442 FAX: 212-746-2009 http://www.cornellphysicians.com/scottely/ Legal Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the original intended recipient(s) selected by Dr. Ely and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the recipient specified by Dr. Ely, please contact the sender by reply e-mail and destroy all copies of the original message. --- From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of histonet-requ...@lists.utsouthwestern.edu [histonet-requ...@lists.utsouthwestern.edu] Sent: Thursday, April 11, 2013 1:17 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 113, Issue 10 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Whole slide scanners, any reviews..? (Erickson, Jamie E) 2. RE: Whole slide scanners, any reviews..? (Elizabeth Chlipala) 3. RE: Whole slide scanners, any reviews..? (Elizabeth Chlipala) 4. Want better IHC / ISH results with 10+ or even 20+ year old FFPE specimens? (Rob Day) 5. Micro-cut H1200 Vibrotome manual (Jo-Ann Bader, Ms.) 6. Talk to me about shelf life and outdates (Cheryl) 7. RE: Talk to me about shelf life and outdates (Elizabeth Chlipala) 8. FFPE for Immunohistochemistry. (Ian R Bernard) 9. Formalin Neutralizer (Bustamante, Lin) 10. compression problems (Denise G Crowley) 11. Re: Formalin Neutralizer (Cristi Rigazio) 12. Re: Talk to me about shelf life and outdates (Emily Sours) 13. RE: compression problems (Elizabeth Chlipala) 14. RE: Talk to me about shelf life and outdates (Goins, Tresa) 15. Re: FFPE for Immunohistochemistry. (Geoff) 16. RE: FFPE for Immunohistochemistry. (Morken, Timothy) 17. Anti-human nuclear antibody (Elizabeth Cameron) 18. RE: Anti-human nuclear antibody (Elizabeth Cameron) 19. Histology Society of Ohio Symposium/Convention April 26-27 (Pam Barker) 20. HM 355S microtome users (Maria Mejia) 21. RE: Anti-human nuclear antibody (Anatoli Gleiberman) -- Message: 1 Date: Wed, 10 Apr 2013 17:17:16 + From: Erickson, Jamie E jamie.erick...@abbvie.com Subject: [Histonet] Whole slide scanners, any reviews..? To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 8b946a68a8f3534a99cc493defb49b10131dd...@wm10002p.oneabbott.com Content-Type:
[Histonet] 35 mm scanners
Everyone, I currently use a Nikon Digital Ice 3, Coolscan IV ED to scan 35 mm photos. Lately, it is often scanning a magenta color or at times either a faded or blurry image and I know I will need to replace it soon. Can anyone recommend a reasonably priced, good quality 35 scanner? It seems they are more difficult to find these days. Your advice would greatly be appreciated. Thank you. Peggy DiCarlo HT(ASCP) Ortho Bone Lab Buffalo General Hospital 100 High Street Buffalo, NY 14203 716-859-1293 The Keeping You Informed section of Kaleida Health`s website features a wealth of information, stories and pictures about our valued workforce and the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please call Kaleida HealthÂs Technology Assistance Center at (716) 859-. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PAP Pen With Aqueous Mounting Media
Hello All, I would like to use a PAP pen to separate sections on my slide, but have been using an aqueous (non-xylenes based) mounting media. How do you remove the PAP pen without using xylenes? Is this done? Thank you, ~Ally Research Technologist Comander Lab - Ocular Genomics Institute Massachusetts Eye Ear Infirmary Lab: (617)-573-6485 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Whole slide scanners.
I think Scott makes a good point about having a clear need. We use the Vectra system now, and have previously used Ariol. We only scan IHC slides from projects in which we want a quantitative analysis. Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott A. Ely Sent: Thursday, April 11, 2013 2:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Whole slide scanners. I have worked with whole slide scanners and digital image analysis since 1998. We currently own scanners from 3 different vendors. Also, I have participated in whole slide scanner marketing research, sitting at a discussion table with a dozen people from different hospitals. My experience has been that most departments purchase scanners without a clear need. For example, some want to scan and archive slides, to make them available for review. That makes some sense in theory. However, in every institution I've talked to, they end up paying for the scanner, a tech to do the work, the service contract and the server space to store the images, which can be huge, up to 20 *giga*bytes per *slide... then find that it is rare for anyone to ever actually look at the scanned images. Most pathologists find it is more expedient to simply pull the glass slides out of the archive. In New York, we are required by law to archive glass slides for at least 10 years anyway. So, my advice, for what it's worth, is not to purchase any whole slide scanner unless you have a clear and well defined need (we have 3 giant systems, rarely used, taking up space and gathering dust). For example, if you need to store images from consultation slides before returning them to another institution, why not just snap a few photomicrographs? Being able to view a couple of small, representative pictures is better than having to wade through an entire scanned slide. --- Scott Ely, MD MPH Associate Director, Hematopathology Fellowship Program Section of Hematopathology Department of Pathology Weill Medical College of Cornell University New York Presbyterian Hospital Room: Starr 715 525 E. 68th Street New York, NY 10065 PH: 212-746-2442 FAX: 212-746-2009 http://www.cornellphysicians.com/scottely/ Legal Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the original intended recipient(s) selected by Dr. Ely and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the recipient specified by Dr. Ely, please contact the sender by reply e-mail and destroy all copies of the original message. --- From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of histonet-requ...@lists.utsouthwestern.edu [histonet-requ...@lists.utsouthwestern.edu] Sent: Thursday, April 11, 2013 1:17 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 113, Issue 10 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Whole slide scanners, any reviews..? (Erickson, Jamie E) 2. RE: Whole slide scanners, any reviews..? (Elizabeth Chlipala) 3. RE: Whole slide scanners, any reviews..? (Elizabeth Chlipala) 4. Want better IHC / ISH results with 10+ or even 20+ year old FFPE specimens? (Rob Day) 5. Micro-cut H1200 Vibrotome manual (Jo-Ann Bader, Ms.) 6. Talk to me about shelf life and outdates (Cheryl) 7. RE: Talk to me about shelf life and outdates (Elizabeth Chlipala) 8. FFPE for Immunohistochemistry. (Ian R Bernard) 9. Formalin Neutralizer (Bustamante, Lin) 10. compression problems (Denise G Crowley) 11. Re: Formalin Neutralizer (Cristi Rigazio) 12. Re: Talk to me about shelf life and outdates (Emily Sours) 13. RE: compression problems (Elizabeth Chlipala) 14. RE: Talk to me about shelf life and outdates (Goins, Tresa) 15. Re: FFPE for Immunohistochemistry. (Geoff) 16. RE: FFPE for Immunohistochemistry. (Morken, Timothy) 17. Anti-human nuclear antibody (Elizabeth Cameron) 18.
Re: [Histonet] PAP Pen With Aqueous Mounting Media
We just leave the pap pen on. Since it's not covering the tissue, it won't matter. Emily By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward. -Chuck Palahniuk, Haunted On Thu, Apr 11, 2013 at 3:29 PM, Langsdorf, Aliete aliete_langsd...@meei.harvard.edu wrote: Hello All, I would like to use a PAP pen to separate sections on my slide, but have been using an aqueous (non-xylenes based) mounting media. How do you remove the PAP pen without using xylenes? Is this done? Thank you, ~Ally Research Technologist Comander Lab - Ocular Genomics Institute Massachusetts Eye Ear Infirmary Lab: (617)-573-6485 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Countertop fume hoods
Hello Histonet! I am looking into small countertop fume hoods or maybe a filter system that I can use to place tissue under that has been in formalin or decalcifier to diminish the fumes. Do you have any recommendation on what and where to look for something like this? Thank you! - Merissa ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Blocks and slide labeling
Hello everyone I have a question about labeling slides and blocks, I run a POL GI lab I was just certified by CAP and I wanted to understand if it is necessary to write GAR13-0123 on both the slides and blocks. We write the patients last name on both, but writing the GAR and an extra 0 is becoming an issue when trying to read the blocks after processing could i write it as G13-123 with the patients last name of course, could I get some advice please Thank you. Madeline Rotger Milanese H.T. BSHCS 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelin...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Formalin Neutralizer
We have a formaldehyde test kit. It's a dip stick type test. Sent from my iPhone On Apr 11, 2013, at 5:31 PM, Mark Tarango marktara...@gmail.com wrote: Can I ask how you test before dumping? Thanks Mark On Apr 11, 2013 6:21 AM, Cristi Rigazio cls71...@gmail.com wrote: We neutralize ours and have no problems with it. I am not sure how much you use, so I will say it is easiest in smaller batches as you do have to shake it up to make sure it dissolves. We purchase ours from BBC Biochemical for a very reasonable price and then test before dumping. We have doing this for four years with no issues. Thanks, Cristi Sent from my iPhone On Apr 11, 2013, at 6:04 AM, Bustamante, Lin lbustama...@cvm.tamu.edu wrote: We are looking into the option of neutralizing our formalin waste instead of having it to be picked up. If you use Formalin Neutralizer, do you have any pro/con about this product? Thank you very much. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas AM University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] - Referrals for Open Position
Hello Histonet! I am actively searching for a night shift Histotech with experience to work in my Shelton, CT lab. Please feel free to reach out to me via email for more details or pass this information onto your contacts. Thank you and I hope everyone is well! Cyndi Cochran Sr. Staffing Specialist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
