[Histonet] Xylene Substitutes
I know this question has been asked before but what's the best and safest Xylene Substitute? Ruth Yaskovich N.I.H. N.I.D.C.R. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Xylene Substitutes
I'm using Clear Rite 3 and I like it. Is there a better xylene substitute for animal tissues? Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edumailto:algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Xylene Substitutes
We use Propar, mostly for mouse and rat eye samples, it works well in our hands Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax l...@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) [algra...@email.arizona.edu] Sent: Wednesday, August 21, 2013 9:47 AM Cc: HISTONET Subject: Re: [Histonet] RE: Xylene Substitutes I'm using Clear Rite 3 and I like it. Is there a better xylene substitute for animal tissues? Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edumailto:algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Xylene Substitutes
Ruth: I all reality none is good. Both alkanes or D-Limonene are either as dangerous or more than xylene, and all perform below the xylene standard. Any one requires modifications of all procedures, they seldom dewax adequately and all constitute a processing compromise. Many cannot de recycled. Please go to http://www.histosearch.com/rene.html and read about xylene substitution. The optimal solution to eliminate xylene consists on: 1- dehydrate with 2-propanol (or pure isopropyl alcohol or IPA) 2- do not use any ante-medium and go from IPA directly to paraffin wax or ideally to mineral oil:IPA at 1:1 3- infiltrate as usual 4- dewax sections with 2%aq. sol. of dishwater soap at 90ºC for 1 min twice 5- stain as usual 6- dehydrate stained sections in a convection oven at 60ºC for 5 minutes 7- coverslip as usual. As you can see xylene is nowhere to be found in this sequence that produce optimal quality slides. Try it! René J. From: Yaskovich, Ruth A (NIH/NIDCR) [E] ryaskov...@dir.nidcr.nih.gov To: 'Histonet@lists.utsouthwestern.edu' Histonet@lists.utsouthwestern.edu Sent: Wednesday, August 21, 2013 11:18 AM Subject: [Histonet] Xylene Substitutes I know this question has been asked before but what's the best and safest Xylene Substitute? Ruth Yaskovich N.I.H. N.I.D.C.R. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Xylene Substitutes
Donna S. Ing= ersoll, B.S., HTL, CT(ASCP) Laboratory Manager A P Laboratories, LLC 2090 Executive Hall Rd S= uite 165 Charleston= , SC 29407 843-300-= 3001 X 202 843-300-= 3003 (fax) [1]dingers...@aplaboratories.com The contents of this message, together = with any attachments, are intended only for the use of the person(s) to whi= ch they are addressed and may contain confidential and/or privileged inform ation. Further, any medical information herein is confidential and protecte= d by law. It is unlawful for unauthorized persons to use, review, copy, dis= close, or disseminate confidential medical information. If you are not the = intended recipient, immediately advise the sender and delete this message a= nd any attachments. Any distribution, or copying of this message, or any at= tachment, is prohibited. Original M= essage Subject: Re: [Histonet] Xylene Substitutes From= : Rene J Buesa [2]rjbu...@yahoo.com Date: Wed, August 21, 2013 12:06 pm To:Yaskovich,Ruth=A(NIH/NIDCR)[E][3]rya skov...@dir.nidcr.nih.gov, '[4]Histonet@lists.utsouthwestern.edu' Histonet@lists.utsouthwest= ern.edu Ruth: I all reality none is good. B= oth alkanes or D-Limonene are either as dangerous or more than xylene, and = all perform below the xylene standard. Any one requires modifications= of all procedures, they seldom dewax adequately and all constitute a proce= ssing compromise. Many cannot de recycled. Please go to [5]http://www.histosearch.com/rene.html= /a and read about xylene substitution. The optimal solu= tion to eliminate xylene consists on: 1- dehydrate with 2-propanol (o= r pure isopropyl alcohol or IPA) 2- do not use any ante-medium and = go from IPA directly to paraffin wax or ideally to mineral oil:IPA at = 1:1 3- infiltrate as usual 4- dewax sections with 2%aq. sol. of= dishwater soap at 90ºC for 1 min twice 5- stain as usual = 6- dehydrate stained sections in a convection oven at 60ºC for 5 minut= es 7- coverslip as usual. As you can see xylene is nowhere to b= e found in this sequence that produce optimal quality slides. Try it! René J. From: Yaskovich, Ruth A (NIH/NIDCR) [E] [6]ryaskov...@dir.nidcr.nih.gov To: '[7]Histonet@lists.utsouthwes= tern.edu' [8]His to...@lists.utsouthwestern.edu Sent: Wednesday, August 21, 2= 013 11:18 AM Subject: [Histonet] Xylene Substitutes = I know this question has been asked before but what's the best and safes= t Xylene Substitute? Ruth Yaskovich N.I.H. N.I.D.C.R. ___= Histonet mailing list [9]histo...@lists.uts= outhwestern.edu [10]http://lists.utsouthwestern.edu/mailman/listinfo/histo= net ___ Histone= t mailing list [11]H= isto...@lists.utsouthwestern.edu [12]http://lists.utsouthwestern.edu/mailm= an/listinfo/histonet References 1. =mailto:dingers...@aplaboratories.com; 2. 3Dmailto:rjbu...@yahoo.com; 3. 3Dmailto:ryaskov...@dir.nidcr.nih.gov; 4. 3Dmailto:Histonet@lists 5. file://localhost/tmp/3D 6. 3Dmailto:ryaskov 7. 3Dmailto:Histonet@lists.utsouthwestern.edu; 8. 3Dmailto:Histonet@lists.utsouthwestern.edu; 9. 3Dmailto:Histonet@lists.utsouthwestern.edu; 10. 3Dhttp://lists.utsouthwestern.edu/mailma 11. 3Dmailto:Histonet@lists.utsouthwestern.edu; 12. 3Dhttp://lists.utsouthw=/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Xylene Substitutes
At another place I've worked for, we used Propar for animal tissues and it worked well. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Wednesday, August 21, 2013 8:59 AM To: Grantham, Andrea L - (algranth) Cc: HISTONET Subject: RE: [Histonet] RE: Xylene Substitutes We use Propar, mostly for mouse and rat eye samples, it works well in our hands Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax l...@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) [algra...@email.arizona.edu] Sent: Wednesday, August 21, 2013 9:47 AM Cc: HISTONET Subject: Re: [Histonet] RE: Xylene Substitutes I'm using Clear Rite 3 and I like it. Is there a better xylene substitute for animal tissues? Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edumailto:algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Agar for rabbit arteries
Hello, I need to order agar to embed rabbit arteries prior to processing. I was told to purchase Difco #0140-01. There are so many different types. What agar do you use and from what vendor do you purchase it? Thank you in advance for your help, Karie -- Karie L Durynski A.S. New Bolton Center University of Pennsylvania School of Veterinary Medicine Comparative Orthopaedic Research Laboratory 382 W Street Road Kennett Square, PA. 19348 Phone:610-925-6278 Fax:610-925-6820 Email:duryn...@vet.upenn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: AW: [Histonet] post-fixation for Mallory Trichrome
Thank you! I have added extra step of 5 min of 1% phosphomolybdic before aniline blue-orangeG, which increased the blue contrast in late stage embryos but not early stage ones. I did not realize that Mallory trichrome react differently between embryonic and adult samples. I just assumed the formalin fixation is causing the staining problem for early embryos. Does formalin fixation cause the different colors or weak staining? If the Mallory Trichrome works differently with developing stages, is mallory trichrome preferably used for adult samples and late stage samples? I found the following ref about modified Mallory Trichrome for embryonic samples. This method is basically use three dyes separately that can help differentiate staining colors like optimized colors achieved in adult samples. I have tried the protocol yet the cartilage does not show sharp enough and most of other tissues are stained purple-ish. Would you suggest some articles that i can refer to for Mallory trichrome in embryos? Thank you, Rui From: gu.l...@gmx.at To: ru...@hotmail.com CC: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] post-fixation for Mallory Trichrome Date: Sat, 17 Aug 2013 09:26:38 +0200 Hi, I think staining would be improved, when you use Bouin as postfixative ( 1h hour, 60°C). Try to invite a further step between Fuchsin and Anilinblue. When you incubate the slides in 1% phosphomolybdic acid for 5-10 min, you can see decolorization of cardilage. This improves later on anilin-binding. You may control the differentiation-step under microscope. Anilinblue can be reduced to 5 min afterwards. You may also consider, that there's a real difference in cardilage-architecture of the early and late samples, that takes influence on staining-abilities. Gudrun Lang -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Rui TAHARA Gesendet: Freitag, 16. August 2013 23:33 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] post-fixation for Mallory Trichrome Hello, There seems to be a lot of suggestions for Mallory Trichrome staining involved in Acid fusin, Aniline blue, and orange G. My sample of avian embryos were fixed with Formalin based fixative (4% paraformaldehyde in phosphate-buffered saline and 1% glutaraldehyde) overnight, in case of late embryos bones were decalcified, and followed standard alcohol series, paraffin embedded, and 10 micron sectioned. The slides were dehydrated, stained with 0.5 % Acid fushin for 10min, then without wash, 0.5% Aniline blue and 2 % orange G in 1% phosphomolybdic acid solution for 20 min, then undergone ethanol series, cleared and mounted. Now I know that formalin fixed sample do not present optimized trichrome colors based on your websites and references. Yet the stained sections of late stage embryo do still show near optimized colors whereas those of early stage embryo show almost no blue or very dark blue, almost gray color for cartilages and most of morphologies as purple-redish colors. Since I tested staining on late embryos first I thought staining would work on early embryos. Does anyone provide me explanation why the staining mostly shows red-ish color and not optimized color for cartilage in early embryos? Is that because of the formalin-fixed embryos although late stage embryos fixed with formalin still show the blue for cartilage? Another question is related to fixative. I prefer not to use bousin fixative due to picric acid and it seems that Citrate buffer or Gram’s iodine solution can be substituted to bousin post-fixative. Have anyone tried these solution for Mallory Trichrome? Do those methods show near optimized color for Mallory trichrome? Any suggestion is appreciated. Thank you, Rui TAHARA PhD candidate McGill University, Biology Department ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Xylene Substitutes
Donna: Please go to the web site I indicated in my previous answer and you find detailed answer. After you stain the sections (regardless of the procedure) you dry them in an oven at 60ºC for 5 mins. and they are completely dry (ANATHEMA!!! to most) you just place them in your automated coverslipper with the same mounting medium you are using now. You need to change NOTHING. Try it! René J. From: dingers...@aplaboratories.com dingers...@aplaboratories.com To: Rene J Buesa rjbu...@yahoo.com; Yaskovich, Ruth A (NIH/NIDCR) [E] ryaskov...@dir.nidcr.nih.gov; 'Histonet@lists.utsouthwestern.edu' Histonet@lists.utsouthwestern.edu Sent: Wednesday, August 21, 2013 12:20 PM Subject: RE: [Histonet] Xylene Substitutes Rene, How do you recommend cover slipping with out xylene? We use a tape cover slip instrument that requires xylene. If you are using glass coverslips what kind of adhesive is used? I am very interested in eliminating xylene as much as possible, but we are a high volume lab and many of the automated instruments (stainers, coverslippers) require xylene. Thanks in advance for your feedback. Donna S. Ingersoll, B.S., HTL, CT(ASCP) Laboratory Manager A P Laboratories, LLC 2090 Executive Hall Rd Suite 165 Charleston, SC 29407 843-300-3001 X 202 843-300-3003 (fax) dingers...@aplaboratories.com The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. Original Message Subject: Re: [Histonet] Xylene Substitutes From: Rene J Buesa rjbu...@yahoo.com Date: Wed, August 21, 2013 12:06 pm To: Yaskovich, Ruth A (NIH/NIDCR) [E] ryaskov...@dir.nidcr.nih.gov, 'Histonet@lists.utsouthwestern.edu' Histonet@lists.utsouthwestern.edu Ruth: I all reality none is good. Both alkanes or D-Limonene are either as dangerous or more than xylene, and all perform below the xylene standard. Any one requires modifications of all procedures, they seldom dewax adequately and all constitute a processing compromise. Many cannot de recycled. Please go to http://www.histosearch.com/rene.html and read about xylene substitution. The optimal solution to eliminate xylene consists on: 1- dehydrate with 2-propanol (or pure isopropyl alcohol or IPA) 2- do not use any ante-medium and go from IPA directly to paraffin wax or ideally to mineral oil:IPA at 1:1 3- infiltrate as usual 4- dewax sections with 2%aq. sol. of dishwater soap at 90ºC for 1 min twice 5- stain as usual 6- dehydrate stained sections in a convection oven at 60ºC for 5 minutes 7- coverslip as usual. As you can see xylene is nowhere to be found in this sequence that produce optimal quality slides. Try it! René J. From: Yaskovich, Ruth A (NIH/NIDCR) [E] ryaskov...@dir.nidcr.nih.gov To: 'Histonet@lists.utsouthwestern.edu' Histonet@lists.utsouthwestern.edu Sent: Wednesday, August 21, 2013 11:18 AM Subject: [Histonet] Xylene Substitutes I know this question has been asked before but what's the best and safest Xylene Substitute? Ruth Yaskovich N.I.H. N.I.D.C.R. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Requisitions
We are having a discussion as to whether the requisition needs to remain with the specimen at the grossing stage or can it be in a separate stack on the grossing table, while the actual specimen is left in the on deck tray. I believe I have seen something that speaks to this through the CAP guidelines, but am unable to find it. Any help would be appreciated. Thanks Gary ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Requisitions
I don't know about CAP guidelines, but I always thought separating the paperwork was to avoid blood/body fluid and/or formaldehyde contamination to the poor transcriptionists and clerical people who have to handle the req after grossing, with no gloves or ventilation! Some places I've worked have put the reqs in clear plastic binder pages to avoid this, in others the transcriptionists have worn gloves. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Wed, Aug 21, 2013 at 1:55 PM, Martin, Gary gmar...@marshallmedical.orgwrote: We are having a discussion as to whether the requisition needs to remain with the specimen at the grossing stage or can it be in a separate stack on the grossing table, while the actual specimen is left in the on deck tray. I believe I have seen something that speaks to this through the CAP guidelines, but am unable to find it. Any help would be appreciated. Thanks Gary ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Requisitions
I am not aware of any CAP regulation that requires the requisition to remain w/ the specimen. The pathologist/PA at gross dissection needs to be able to positively confirm/verify the specimen container information (two forms of patient ID and specimen source) w/ the order entry information (taken from the requisition). There are many labs that are paperless during the technical process and I see more and more use of electronic requisitions and orders. William DeSalvo, BS HTL(ASCP) Chair, NSH Quality Management Committee Date: Wed, 21 Aug 2013 11:55:12 -0700 From: gmar...@marshallmedical.org To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Requisitions We are having a discussion as to whether the requisition needs to remain with the specimen at the grossing stage or can it be in a separate stack on the grossing table, while the actual specimen is left in the on deck tray. I believe I have seen something that speaks to this through the CAP guidelines, but am unable to find it. Any help would be appreciated. Thanks Gary ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Requisitions
There are times when the requisition and the specimen are labeled differently. Many offices pre-label their specimen containers, take the specimen from a different source and forget to change the label on the container. They make the change on the requisition and then you have a discrepancy. The grosser is a perfect double check to make sure everything matches. It will keep you from having to amend reports that go out before the discrepancy is discovered. Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.por...@caplab.org www.caplab.org The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Wednesday, August 21, 2013 4:02 PM To: Martin, Gary; histonet Subject: RE: [Histonet] Requisitions I am not aware of any CAP regulation that requires the requisition to remain w/ the specimen. The pathologist/PA at gross dissection needs to be able to positively confirm/verify the specimen container information (two forms of patient ID and specimen source) w/ the order entry information (taken from the requisition). There are many labs that are paperless during the technical process and I see more and more use of electronic requisitions and orders. William DeSalvo, BS HTL(ASCP) Chair, NSH Quality Management Committee Date: Wed, 21 Aug 2013 11:55:12 -0700 From: gmar...@marshallmedical.org To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Requisitions We are having a discussion as to whether the requisition needs to remain with the specimen at the grossing stage or can it be in a separate stack on the grossing table, while the actual specimen is left in the on deck tray. I believe I have seen something that speaks to this through the CAP guidelines, but am unable to find it. Any help would be appreciated. Thanks Gary ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Requisitions
That seems to happen pretty often. When I accessioned a lot it was at least a daily occurrence that information would not match between the jar and requisition, or information was missing or illegible. I have noticed that it is much easier to reach the actual person who labeled the specimen for corrections if you attempt to track them down as soon as possible after the specimen is dropped off. I have a policy for checking the manifest and then cross checking the jar and requisition during accessioning that requires they travel together in order to be cross-checked. If problems are noted, discrepancies, missing information and the like, there is a form to document the discrepancies and relabeling needed. I prefer to also note in the LIS, just in case there are still questions later. I just feel that even if it is not specifically stipulated in regulations or checklists that when the requisition and specimen/specimen jar are always together you can catch specimen integrity problems sooner so they can be corrected. The grosser is also a great checkpoint, definitely. Joelle Weaver MAOM, HTL (ASCP) QIHC From: doug.por...@caplab.org To: histonet@lists.utsouthwestern.edu Date: Wed, 21 Aug 2013 16:19:10 -0400 Subject: RE: [Histonet] Requisitions There are times when the requisition and the specimen are labeled differently. Many offices pre-label their specimen containers, take the specimen from a different source and forget to change the label on the container. They make the change on the requisition and then you have a discrepancy. The grosser is a perfect double check to make sure everything matches. It will keep you from having to amend reports that go out before the discrepancy is discovered. Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.por...@caplab.org www.caplab.org The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Wednesday, August 21, 2013 4:02 PM To: Martin, Gary; histonet Subject: RE: [Histonet] Requisitions I am not aware of any CAP regulation that requires the requisition to remain w/ the specimen. The pathologist/PA at gross dissection needs to be able to positively confirm/verify the specimen container information (two forms of patient ID and specimen source) w/ the order entry information (taken from the requisition). There are many labs that are paperless during the technical process and I see more and more use of electronic requisitions and orders. William DeSalvo, BS HTL(ASCP) Chair, NSH Quality Management Committee Date: Wed, 21 Aug 2013 11:55:12 -0700 From: gmar...@marshallmedical.org To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Requisitions We are having a discussion as to whether the requisition needs to remain with the specimen at the grossing stage or can it be in a separate stack on the grossing table, while the actual specimen is left in the on deck tray. I believe I have seen something that speaks to this through the CAP guidelines, but am unable to find it. Any help would be appreciated. Thanks Gary ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
