[Histonet] Low Temp problem

[Gary Gracie]

I am also running Ventana Benchmark Ultras. This low temp problem has started 
to occur regularly to individual heater pads (five in total over several weeks, 
across two machines). Ventana have promptly replaced the pad on each occasion.

Gary Gracie
Senior Technical Officer
IHC Laboratory
Anatomical Pathology
St Vincents Hospital
Sydney, Australia

Message: 1
Date: Tue, 22 Oct 2013 11:04:45 -0700
From: "HERRINGTON, SHEILA" 
Subject: RE: [Histonet] Low Temp question
To: 'Tom McNemar' , 'Shawn Leslie '
, "Histonet@lists.utsouthwestern.edu"

Message-ID:

<9d5f3f245a5fe0458e46e9a71e3ee08c06cd37f...@dc1serv352.interiorhealth.ca>

Content-Type: text/plain; charset="utf-8"

I also have been having low temp errors.  I use  Benchmark Ultras and they are 
not a batch stainer, so pads are heated independently.  I noticed the staining 
on the particular pad with the error was not quite to the usual standard so I 
have stopped using that pad.  Have contacted Ventana for a case number, but 
have not got an answer.  Is this a new problem for you as well?  This only 
started a few weeks ago for us.


Sheila Herrington
Technical Lead Histopathology and Immunohistochemistry
Kelowna General Hospital
2268 Pandosy Street, Kelowna, B.C. V1Y 1T2
250-862-4300 ext 7587 or 7510
sheila.herring...@interiorhealth.ca





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar
Sent: Tuesday, October 22, 2013 8:28 AM
To: 'Shawn Leslie '; Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Low Temp question

We see these continually as well.  We are told that it is a software glitch and 
not really a problem with the heat. I was told that they are working on it.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shawn Leslie
Sent: Tuesday, October 22, 2013 11:07 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Low Temp question


Goodmorning all,
I have a question concerning the Ventana Benchmark XT. We are constantly 
having low temperature errors during almost every run. The immunos look fine 
but we are still concerned. Has anyone else that uses the Benchmark experienced 
this also ? We have noticed that the silver coating on the heating pads is 
wearing off. Ventana indicated to us that it's nothing to be concerned about. 
Any hel p would be appreciated.

Shawn Leslie HT (ASCP)

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[Histonet] RE: cutting thick brain frozen sections for Golgi stain

[Entwistle, Laura]

I routinely cut 50 or even 200um sections.  The brain should have some 
variations of light beige to it.  If it is too white, it is too cold.  What are 
you using?  I have found that a microtome works much better for thicker slices 
than a cryostat.

Good luck!
Laura

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kruttwig, Klaus
Sent: Wednesday, October 23, 2013 3:22 PM
To: Grantham, Andrea L - (algranth); HISTONET
Subject: [Histonet] RE: cutting thick brain frozen sections for Golgi stain

Hi Andrea,

did you try it with a vibratome? If not, I would recommend it.

Thanks,
Klaus

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Grantham, Andrea L - 
(algranth) [algra...@email.arizona.edu]
Sent: Wednesday, October 23, 2013 3:01 PM
To: HISTONET
Subject: [Histonet] cutting thick brain frozen sections for Golgi stain

I need to cut 80 micron sections of frozen brain.
Tried today at -18 degrees C but the brains kept cracking. I read that the temp 
should be -3 to -13 degrees C. Has anybody cut sections this thick of brain? 
Can you pass along any words of inspiration?



Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algra...@email.arizona.edu
Tel: 520.626.4415 Fax: 520.626.2097





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[Histonet] RE: cutting thick brain frozen sections for Golgi stain

[Kruttwig, Klaus]

Hi Andrea,

did you try it with a vibratome? If not, I would recommend it.

Thanks,
Klaus

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Grantham, Andrea L - 
(algranth) [algra...@email.arizona.edu]
Sent: Wednesday, October 23, 2013 3:01 PM
To: HISTONET
Subject: [Histonet] cutting thick brain frozen sections for Golgi stain

I need to cut 80 micron sections of frozen brain.
Tried today at -18 degrees C but the brains kept cracking. I read that the temp 
should be -3 to -13 degrees C. Has anybody cut sections this thick of brain? 
Can you pass along any words of inspiration?



Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algra...@email.arizona.edu
Tel: 520.626.4415 Fax: 520.626.2097





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[Histonet] cutting thick brain frozen sections for Golgi stain

[Grantham, Andrea L - (algranth)]

I need to cut 80 micron sections of frozen brain.
Tried today at -18 degrees C but the brains kept cracking. I read that the temp 
should be -3 to -13 degrees C. Has anybody cut sections this thick of brain? 
Can you pass along any words of inspiration?



Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algra...@email.arizona.edu
Tel: 520.626.4415 Fax: 520.626.2097





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RE: [Histonet] HTL / CG

[Morken, Timothy]

Joelle, I think you will have a very hard time finding someone with both, 
especially dual ASCP certification. I've met or hear of anyone with dual 
certification, or even working in both. It may be the best you will find is 
someone who has certification in one and some practical experience in the 
other. Or someone willing to cross-train. 

Good luck!


Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
Sent: Wednesday, October 23, 2013 10:35 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] HTL / CG

 Hello fellow histology netters
I have been asked by my employer to search for and retain an individual who 
possesses an HTL (ASCP) certification, but who also has a CG (ASCP) 
certification ( or at least have solid experience in the arena of FISH & 
cytogenetics).  I have not personally come across anyone like that in my own 
personal, mostly clinical histology career. Perhaps it is more common in 
research?
 
Can anyone offer an opinion or insight into how common the above combination of 
education, training and certification(s) may be?
I tried to contact the BOR/BOC for a non-identified statistic on that, but have 
not gotten a reply.
Appreciate any assistance.
 



Joelle Weaver MAOM, HTL (ASCP) QIHC
 
> From: mckenzie.em...@mhsil.com
> To: histonet@lists.utsouthwestern.edu
> Date: Wed, 16 Oct 2013 19:31:20 -0500
> Subject: [Histonet] Desperately seeking information!!!
> 
> Hello all,
> A few weeks ago I sent out an information seeking email regarding IHC 
> turnaround time. I did not get much in the way of responses. I figured there 
> was not enough information provided to answer the general questions I was 
> asking. I am having trouble obtaining an national average for IHC turnaround 
> time.
> I am wondering if all you fellow histoneters out there would be willing to 
> give me some info so I can see were we stand in comparison to facilities of 
> similar size. The facility I work at turns out anywhere from 90-150 IHC 
> stained slides daily. We have an average of 160 cases with around 700 H&E 
> stained slides daily. I have listed a few questions below, if any of you 
> would be so kind as to take the time to answer them it would be greatly 
> appreciated.
> 
> What is the rough estimate of cases and initial H&E stained slides that are 
> turned out daily?
> 
> Roughly, how many IHC stained slides do you turn out in a day?
> 
> On average, what is your IHC turnaround time?
> 
> What tissues are you working with (general surgical, dermatology's, research 
> etc)?
> 
> How many techs do you have that can perform IHC staining?
> 
> Who is your instrumentation through?
> 
> At the end of the day/run, is there a stain log printed?
> 
> If so, who signs off on the positive/negative?
> 
> If there are any other processes/procedures you feel are imperative to your 
> IHC turnaround time please feel free to comment or offer suggestions.
> 
> Thank you for taking the time to help us to improve our processes. If you 
> have any questions or concerns please let me know.
> Again, thank you for your help,
> 
> 
> Emily K. McKenzie BS, HT(ASCP)
> 
> Memorial Medical Center│701 North First Street│Springfield, IL 62781
> Ph: 217-788-3991│email: mckenzie.em...@mhsil.com
> 
> 
> 
> 
>   
> This message (including any attachments) contains confidential information 
> intended for a specific individual and purpose, and is protected by law. If 
> you are not the intended recipient, you should delete this message. Any 
> disclosure, copying, or distribution of this message, or the taking of any 
> action based on it, is strictly prohibited.
> ___
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RE: [Histonet] HTL / CG

[joelle weaver]

Thank you for the information and insight. You are indeed multi-talented. 
Sounds like whatever direction you go, you will be successful. I am not 
expecting to find such a combination as HTL/CG, with extensive experience 
easily. I just wanted to have some numbers, opinions,  and information to 
return if it takes a long while- and some kind of explanation for being 
empty-handed when them come asking.
 




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
> From: taylor.cliff...@va.gov
> To: joellewea...@hotmail.com
> Date: Wed, 23 Oct 2013 15:22:34 -0400
> Subject: RE: [Histonet] HTL / CG
> 
> I would say pretty uncommon! 
> 
> I graduated with my Bachelor's in Agricultural Biotechnology and as a side 
> bar did my AAS in Histotechnology so my current supervisor was incredibly 
> pleased with the fact that I had a solid background in both biomolecular 
> science and current biotechnology techniques as well as the 
> histology/histotechnology background. He's the PI of a neuropathology lab 
> where he has done basic and advanced immunohistochemistry and various 
> biomolecular testing for 40+ years and he said I was the first HT to also 
> have the biotechnology background.
> 
> I graduated from SUNY Cobleskill where Dr. Colony has been the program 
> director for a number of years and I was also her first student to do both 
> programs (Benefitted the program greatly to have a student tutor the 
> following year on campus since most students finish their AAS and are gone). 
> I have looked into continuing my education for the CG  certification but I'm 
> still getting my foot in the door here at the research lab and studying for 
> GRE's for entrance into either a PhD or DVM program so I don't want to add 
> any more to my plate at the moment!
> 
> Good luck on your search!!
> 
> 
> Taylor CM Clifford
> Research Associate
> Albany Research Institute
> 113 Holland Avenue
> Albany, NY 12208
> 518-626-5664
> taylor.cliff...@va.gov
> 
> 
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
> Sent: Wednesday, October 23, 2013 1:35 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] HTL / CG
> 
>  Hello fellow histology netters
> I have been asked by my employer to search for and retain an individual who 
> possesses an HTL (ASCP) certification, but who also has a CG (ASCP) 
> certification ( or at least have solid experience in the arena of FISH & 
> cytogenetics).  I have not personally come across anyone like that in my own 
> personal, mostly clinical histology career. Perhaps it is more common in 
> research?
>  
> Can anyone offer an opinion or insight into how common the above combination 
> of education, training and certification(s) may be?
> I tried to contact the BOR/BOC for a non-identified statistic on that, but 
> have not gotten a reply.
> Appreciate any assistance.
>  
> 
> 
> 
> Joelle Weaver MAOM, HTL (ASCP) QIHC
>  
> > From: mckenzie.em...@mhsil.com
> > To: histonet@lists.utsouthwestern.edu
> > Date: Wed, 16 Oct 2013 19:31:20 -0500
> > Subject: [Histonet] Desperately seeking information!!!
> > 
> > Hello all,
> > A few weeks ago I sent out an information seeking email regarding IHC 
> > turnaround time. I did not get much in the way of responses. I figured 
> > there was not enough information provided to answer the general questions I 
> > was asking. I am having trouble obtaining an national average for IHC 
> > turnaround time.
> > I am wondering if all you fellow histoneters out there would be willing to 
> > give me some info so I can see were we stand in comparison to facilities of 
> > similar size. The facility I work at turns out anywhere from 90-150 IHC 
> > stained slides daily. We have an average of 160 cases with around 700 H&E 
> > stained slides daily. I have listed a few questions below, if any of you 
> > would be so kind as to take the time to answer them it would be greatly 
> > appreciated.
> > 
> > What is the rough estimate of cases and initial H&E stained slides that are 
> > turned out daily?
> > 
> > Roughly, how many IHC stained slides do you turn out in a day?
> > 
> > On average, what is your IHC turnaround time?
> > 
> > What tissues are you working with (general surgical, dermatology's, 
> > research etc)?
> > 
> > How many techs do you have that can perform IHC staining?
> > 
> > Who is your instrumentation through?
> > 
> > At the end of the day/run, is there a stain log printed?
> > 
> > If so, who signs off on the positive/negative?
> > 
> > If there are any other processes/procedures you feel are imperative to your 
> > IHC turnaround time please feel free to comment or offer suggestions.
> > 
> > Thank you for taking the time to help us to improve our processes. If you 
> > have any questions or concerns please let me know.
> > Again, thank you for your help,
> > 
> > 
> > Emily K. McKenzie BS, HT(ASCP)
> > 
> > Memorial Medical Center│701 Nort

Re: [Histonet] RE: Can someone recommend a good labeling pen for cryotubes.

[Patrick Laurie]

I second the statmark pens from stat-lab. They are very solvent resistant,
don't dry out that easy.  They are however a little on the pricey side, at
about $5.00 a pen.

Patrick Laurie


On Wed, Oct 23, 2013 at 1:58 PM, Walter Benton  wrote:

> Are you using the red label "Industrial Sharpie" already, if not give them
> a try?
>
> I would also suggest using a slide marking pen since they are resistant to
> many solvents used in the lab like alcohol and xylene.
>
> Walter Benton HT(ASCP)QIHC
> Histology Supervisor
> Chesapeake Urology Associates
> 806 Landmark Drive, Suite 127
> Glen Burnie, MD 21061
> 443-471-5850 (Direct)
> 410-768-5961 (Lab)
> 410-768-5965 (Fax)
> ChesapeakeUrology.com
>
> Voted a Best Place to Work by
> Baltimore and Modern Healthcare
> Magazines.
> 
> From: histonet-boun...@lists.utsouthwestern.edu [
> histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick [
> patrick.le...@seattlechildrens.org]
> Sent: Wednesday, October 23, 2013 1:54 PM
> To: 'Histonet@lists.utsouthwestern.edu'
> Subject: [Histonet] Can someone recommend a good labeling pen for
> cryotubes.
>
> Hi everyone,
>
> Can someone recommend a good pen to use to label cryo tubes.The VWR
> pens we have been using are apparently discontinued.
>
> I would like something that writes as fine as an ultrafine sharpie, but is
> less likely to smear and is more long lasting, and solvent tolerant.
>
> Thanks
>
> Patrick.
>
>
> CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is
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> disclosure or distribution is prohibited. If you are not the intended
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>
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> message is intended solely for the personal and confidential use of the
> designated recipient(s) named above and may contain information that is
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-- 

Patrick Laurie(HT)ASCP QIHC

Histology Manager

Celligent Diagnostics, LLC

101 East W.T. Harris Blvd  | Suite 1212 | Charlotte, NC 28262

Work: 704-970-3300  Cell: 704-266-0869
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RE: [Histonet] CLIA

[Hale, Meredith]

Thanks Kim, 

 I agree seems more clinical ... 

-Original Message-
From: Kim Donadio [mailto:one_angel_sec...@yahoo.com] 
Sent: Wednesday, October 23, 2013 1:22 PM
To: Hale, Meredith
Cc: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] CLIA 

I can only speak from a path lab point of view and from reading the IQCP 
guidelines it doesn't allow you to use these new measures for path. Path is 
exempt. and in path I've always used controls for each test. My understanding 
is in some specialties they are going to let them make individualized qc 
measures if they want to do  those risk assessments etc. I'm still going over 
it but this is the just of what I see so far. Hope this helps. 

Sent from my iPhone

On Oct 22, 2013, at 10:23 AM, "Hale, Meredith"  wrote:

> Has anyone out there been told by CLIA you must run a negative control along 
> with a positive control for HP special stains , not IHC ?  I know we do this 
> for AFB but never have for an HP special stain .
> 
> Also the new IQCP CLIA is rolling out  it states that if your lab doesn't 
> enroll in the voluntary IQCP that you must perform tow external controls on 
> each test system for each day . Is this what they may be referring to ? 
> Thanks in advance .
> 
> 
> 
> Meredith Hale HT  (ASCP)cm
> Director External Sales Support
> 
> Miraca Life Sciences
> 6655 North MacArthur Blvd.
> Irving , Texas 75039
> Office: 214-596-2219
> Cell: 469-648-8253
> Fax: 1-866-688-3280
> mh...@miracals.com>
> 
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Re: [Histonet] CLIA

[Kim Donadio]

I can only speak from a path lab point of view and from reading the IQCP 
guidelines it doesn't allow you to use these new measures for path. Path is 
exempt. and in path I've always used controls for each test. My understanding 
is in some specialties they are going to let them make individualized qc 
measures if they want to do  those risk assessments etc. I'm still going over 
it but this is the just of what I see so far. Hope this helps. 

Sent from my iPhone

On Oct 22, 2013, at 10:23 AM, "Hale, Meredith"  wrote:

> Has anyone out there been told by CLIA you must run a negative control along 
> with a positive control for HP special stains , not IHC ?  I know we do this 
> for AFB but never have for an HP special stain .
> 
> Also the new IQCP CLIA is rolling out  it states that if your lab doesn't 
> enroll in the voluntary IQCP that you must perform tow external controls on 
> each test system for each day . Is this what they may be referring to ? 
> Thanks in advance .
> 
> 
> 
> Meredith Hale HT  (ASCP)cm
> Director External Sales Support
> 
> Miraca Life Sciences
> 6655 North MacArthur Blvd.
> Irving , Texas 75039
> Office: 214-596-2219
> Cell: 469-648-8253
> Fax: 1-866-688-3280
> mh...@miracals.com>
> 
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[Histonet] RE: ASR antibodies verses IVD antibodies

[Morken, Timothy]

Jim, Since companies are not allowed, by law, to tell you any method 
information about ASR's, it is the responsibility of the lab to do a 
comprehensive validation according to CLIA regulations. Treat it as if it were 
an antibody some researcher gave you and you need to figure out EVERYTHING to 
complete a validation:  specificity and sensitivity, reproducibility, etc. That 
all has to be documented.

Tim Morken
UCSF Pathology

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim
Sent: Wednesday, October 23, 2013 10:37 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] ASR antibodies verses IVD antibodies


In older CAP regulations ASR's had to be handled separately than IVD antibodies 
including a disclaimer acquired from the FDA.   I don't find that in the 
current CAP checklist.  Has this been eliminated and if so how are people 
handling validation of ASR antibodies?   Are they using the validation they use 
for new antibodies introduced to the lab?

Jim

James Vickroy BS, HT(ASCP)

Surgical  and Autopsy Pathology Technical Supervisor Memorial Medical Center
217-788-4046



This message (including any attachments) contains confidential information 
intended for a specific individual and purpose, and is protected by law. If you 
are not the intended recipient, you should delete this message. Any disclosure, 
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[Histonet] RE: Can someone recommend a good labeling pen for cryotubes.

[Walter Benton]

Are you using the red label "Industrial Sharpie" already, if not give them a 
try?

I would also suggest using a slide marking pen since they are resistant to many 
solvents used in the lab like alcohol and xylene.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
ChesapeakeUrology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick 
[patrick.le...@seattlechildrens.org]
Sent: Wednesday, October 23, 2013 1:54 PM
To: 'Histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Can someone recommend a good labeling pen for cryotubes.

Hi everyone,

Can someone recommend a good pen to use to label cryo tubes.The VWR pens we 
have been using are apparently discontinued.

I would like something that writes as fine as an ultrafine sharpie, but is less 
likely to smear and is more long lasting, and solvent tolerant.

Thanks

Patrick.


CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for 
the sole use of the intended recipient(s) and may contain confidential and 
privileged information protected by law. Any unauthorized review, use, 
disclosure or distribution is prohibited. If you are not the intended 
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the original message.

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are hereby notified that any dissemination, distribution or copying of this 
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telephone (410) 581-5881 and delete the message without making a copy.

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[Histonet] RE: Can someone recommend a good labeling pen for cryotubes.

[Weems, Joyce K.]

We have had good luck with the Statmark Pen from StatLab - Item # SMP-BK.

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick
Sent: Wednesday, October 23, 2013 1:54 PM
To: 'Histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Can someone recommend a good labeling pen for cryotubes.

Hi everyone,

Can someone recommend a good pen to use to label cryo tubes.The VWR pens we 
have been using are apparently discontinued.

I would like something that writes as fine as an ultrafine sharpie, but is less 
likely to smear and is more long lasting, and solvent tolerant.

Thanks

Patrick.


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[Histonet] Can someone recommend a good labeling pen for cryotubes.

[Lewis, Patrick]

Hi everyone,

Can someone recommend a good pen to use to label cryo tubes.The VWR pens we 
have been using are apparently discontinued.

I would like something that writes as fine as an ultrafine sharpie, but is less 
likely to smear and is more long lasting, and solvent tolerant.

Thanks

Patrick.


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disclosure or distribution is prohibited. If you are not the intended 
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the original message.

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[Histonet] ASR antibodies verses IVD antibodies

[Vickroy, Jim]

In older CAP regulations ASR's had to be handled separately than IVD antibodies 
including a disclaimer acquired from the FDA.   I don't find that in the 
current CAP checklist.  Has this been eliminated and if so how are people 
handling validation of ASR antibodies?   Are they using the validation they use 
for new antibodies introduced to the lab?

Jim

James Vickroy BS, HT(ASCP)

Surgical  and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046



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[Histonet] HTL / CG

[joelle weaver]

 Hello fellow histology netters
I have been asked by my employer to search for and retain an individual who 
possesses an HTL (ASCP) certification, but who also has a CG (ASCP) 
certification ( or at least have solid experience in the arena of FISH & 
cytogenetics).  I have not personally come across anyone like that in my own 
personal, mostly clinical histology career. Perhaps it is more common in 
research?
 
Can anyone offer an opinion or insight into how common the above combination of 
education, training and certification(s) may be?
I tried to contact the BOR/BOC for a non-identified statistic on that, but have 
not gotten a reply.
Appreciate any assistance.
 



Joelle Weaver MAOM, HTL (ASCP) QIHC
 
> From: mckenzie.em...@mhsil.com
> To: histonet@lists.utsouthwestern.edu
> Date: Wed, 16 Oct 2013 19:31:20 -0500
> Subject: [Histonet] Desperately seeking information!!!
> 
> Hello all,
> A few weeks ago I sent out an information seeking email regarding IHC 
> turnaround time. I did not get much in the way of responses. I figured there 
> was not enough information provided to answer the general questions I was 
> asking. I am having trouble obtaining an national average for IHC turnaround 
> time.
> I am wondering if all you fellow histoneters out there would be willing to 
> give me some info so I can see were we stand in comparison to facilities of 
> similar size. The facility I work at turns out anywhere from 90-150 IHC 
> stained slides daily. We have an average of 160 cases with around 700 H&E 
> stained slides daily. I have listed a few questions below, if any of you 
> would be so kind as to take the time to answer them it would be greatly 
> appreciated.
> 
> What is the rough estimate of cases and initial H&E stained slides that are 
> turned out daily?
> 
> Roughly, how many IHC stained slides do you turn out in a day?
> 
> On average, what is your IHC turnaround time?
> 
> What tissues are you working with (general surgical, dermatology's, research 
> etc)?
> 
> How many techs do you have that can perform IHC staining?
> 
> Who is your instrumentation through?
> 
> At the end of the day/run, is there a stain log printed?
> 
> If so, who signs off on the positive/negative?
> 
> If there are any other processes/procedures you feel are imperative to your 
> IHC turnaround time please feel free to comment or offer suggestions.
> 
> Thank you for taking the time to help us to improve our processes. If you 
> have any questions or concerns please let me know.
> Again, thank you for your help,
> 
> 
> Emily K. McKenzie BS, HT(ASCP)
> 
> Memorial Medical Center│701 North First Street│Springfield, IL 62781
> Ph: 217-788-3991│email: mckenzie.em...@mhsil.com
> 
> 
> 
> 
>   
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> intended for a specific individual and purpose, and is protected by law. If 
> you are not the intended recipient, you should delete this message. Any 
> disclosure, copying, or distribution of this message, or the taking of any 
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[Histonet] Amacr antibody from DAKO

[Vickroy, Jim]

I am checking into ordering the prediluted amacr antibody from DAKO since it is 
an IVD.   Could anyone share the protocol they are using on the Benchmark Ultra 
with this antibody and the standard I-view detection kit?

James Vickroy BS, HT(ASCP)

Surgical  and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046



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Re: [Histonet] slide drying oven

[Patrick Laurie]

Hi Laurie,

Biocare has one that I have used, they call it the desert chamber pro, one
of the few that is setup and designed expressly for drying slides.  It
works very well, and has different heating options and a timer.  However,
all you may need is a basic mechanical convection oven, Boekel has a couple
of what they call digital incubators with a fan that can go from ambient to
90 degrees. Finally, if cost is a consideration, I've purchased from
Affordableovens.com. I got a relatively inexpensive mechanical convection
oven for $1500 that works well.

Good luck,

Patrick Laurie
Celligent Diagnostics
Charlotte, NC


On Wed, Oct 23, 2013 at 10:59 AM, Laurie Colbert wrote:

> Can anyone recommend a small, forced air slide drying oven?
>
> Laurie Colbert, HT (ASCP)
> Histology Supervisor
> PATH MD
> 8158 Beverly Blvd.
> Los Angeles, CA  90048
> (323) 648-3214 direct
> (424) 245-7284 main lab
>
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-- 

Patrick Laurie(HT)ASCP QIHC

Histology Manager

Celligent Diagnostics, LLC

101 East W.T. Harris Blvd  | Suite 1212 | Charlotte, NC 28262

Work: 704-970-3300  Cell: 704-266-0869
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[Histonet] slide drying oven

[Laurie Colbert]

Can anyone recommend a small, forced air slide drying oven?

Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA  90048
(323) 648-3214 direct
(424) 245-7284 main lab

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[Histonet] Shedding biopsies

[Davis, Cassie]

Hi Diana,
RE: "In the past when we receive these samples there has been no issues cutting 
them.  Over the past few months there has been a tendency for these blocks to 
shred into fine strips when cut.  Almost like there was sand in the blocks.  
Mollifex, decal, warm or cold, doesn't matter they still shred.  Getting levels 
is such a challenge.  I have inquired at the collection site to determine if 
there is a change in the process of collecting that could cause this.  I have 
ensured the samples are not left on gauze or something to allow them to dry 
out. Even blocks with lots of blood and/or mucous will shred as well as blocks 
with only a scanty amount present."



There was a problem a few/many years ago with a paraffin manufacture using a 
cardboard conveyor belt for the pellets. Apparently, small pieces of cardboard 
making it's way in with the paraffin. You can check your melting tub and see if 
there is a tan residual in the bottom this will let you know if "foreign" 
material may be responsible for your problem.



Cassandra Davis





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[Histonet] New Technical Sales Opportunity - IHC Antibodies

[Matt Ward]

Good morning, 

 

We have had a world leading cancer diagnostics client open a new opportunity
in the Southwest. Our client is searching for an Antibody Sales Specialist
ideally based in TX, OK, AR, LA, or AZ. We are looking for histology
professionals who have a strong background in IHC and antibodies, to drive
antibodies sales in the region. 

 

Please contact me directly to learn more about the opportunity.
m...@personifysearch.com  , or 800.875.6188
ext. 103

 

Thank you, 

 

Matt Ward

Account Executive

Personify

5020 Weston Parkway Suite 315

Cary NC 27513 

(Tel) 800.875.6188 direct ext 103

(Fax) 919.460.0642

   www.personifysearch.com

 

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