[Histonet] Leica MC120HD / MC170HD microscope cameras
Hello! I'm trying to find a new microscope camera for our histology histopathology teaching lab. I would like to achieve full HD live image with good color reproduction. Anyone have experience on the Leica MC120/MC170 HD cameras? They are quite nice otherwise, but based on a quick demo, I'm not 100% convinced with the color quality. Recommendations for other cameras are also appreciated. With best regards, Mikael Niku, PhD University of Helsinki, Finland ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Yahoo link
I know the potential for damage to your health is huge in histology, but are there any studies out there that indicate histotechs are less healthy than the average person? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Tuesday, December 03, 2013 7:06 PM To: Histonet Subject: [Histonet] RE: Yahoo link Old Histologists never die, they're just well fixed... Claire From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Morken, Timothy [timothy.mor...@ucsfmedctr.org] Sent: Tuesday, December 03, 2013 11:22 AM To: 'Shirley A. Powell'; Histonet Subject: [Histonet] RE: Yahoo link Well, Shirley, you are actually an Angel, so nothing will ever stop you!! (from an old Georgia Society hand). Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Tuesday, December 03, 2013 9:18 AM To: Histonet Subject: [Histonet] Yahoo link When I entered the profession I was told the average life expectancy of a histotechs was 20 years from hiring. That scared me but I was already hooked. I have been doing this 51 years, so maybe good laboratory practices can help, in spite of bad ventilation and all those other dangers mentioned. Shirley Powell Antique Histotech -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Tuesday, December 03, 2013 11:40 AM To: Morken, Timothy; Histonet Subject: Re: [Histonet] RE: Yahoo link Just what I was thinking. We will never get enough new students now! With proper training and equipment, we do the job safely! And you can drown in an inch of water. Is that in the MSDS! Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com From: Morken, Timothy timothy.mor...@ucsfmedctr.org To: 'Mike Tighe' mti...@trudeauinstitute.org; histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) histonet@lists.utsouthwestern.edu Sent: Tuesday, December 3, 2013 10:24 AM Subject: [Histonet] RE: Yahoo link Great. Just what we need. Even so, we in the business can take these lists with a grain of K4[Fe(CN) 6] * 3H 2 O since we know that suitable precautions preclude most of the danger. For instance, I'll have to say that the histo lab here is wonderful in that it has such good ventilation (ie., extraction) that there is none of the usual chemical smell - no xylene, alcohol, specials chemicals that often assaults the senses in histology. Vendors that come here are amazed. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Tighe Sent: Tuesday, December 03, 2013 6:31 AM To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Yahoo link Anybody wonder who has the most harzardous job to your health? We're Number One!!! http://finance.yahoo.com/news/the-15-jobs-that-are-most-damaging-to-your-health-155706120.html Mike ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu
[Histonet] Histology Leader Webinars
If not interested in webinars designed for supervisors and trainers/instructors, please delete. NSH has developed a new set of webinars for supervisors and instructors. 11 in total in 2014 – 3 on management, 3 on education/training, 5 on quality. The webinars are $35 per person if ordered individually. Discounts: If ordering: - all 11 = $325 - 3 management = $90 - 3 education = $90 - 5 quality = $145 - pick any 6 = $180 1 hour each, 1 hour CE if attend the webinar (will get an archive version later for reference, but cannot attend later and earn CE). 1-2 pm ET, various days of the week. For more information: http://www.nsh.org/content/2014-histology-leader-webinars Click on “Click here to register now” On top, click on “Webinars” to get titles, speakers, abstracts, dates Disclosure: I’m the NSH webinar coordinator. Non-paid position. Just working with NSH to bring educational material to supervisors and instructors in histotechnology. Peggy A. Wenk, HTL(ASCP)SLS ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Looking for histotechnologist is NYC
Hi All, I'm currently recruiting for a histotechnologist in NYC. Must have NY State Clinical Laboratory Technologist License and a Bachelor's degree for a 2nd shift opportunity. Please call/email with any questions. Anna Nolan - Recruiter Prometheus Healthcare Direct Line 301-693-8908 Office 301-693-9057 Fax 301-368-2478 http://ano...@prometheushealthcare.com/ anolan mailto:ch...@prometheushealthcare.com @prometheushealthcare.com http://www.linkedin.com/pub/annelise-nolan/55/ba0/ab6 http://www.linkedin.com/pub/annelise-nolan/55/ba0/ab6 http://www.linkedin.com/profile/view?id=197035050 http://www.prometheushealthcare.com/ www.prometheushealthcare.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Yahoo link
I agree! If everyone adheres to safe practices we will make it into our golden years. Let's bring back our NSH health studies~ Vivian McClure would love for us to continue the studies. Mike Ayers and Shirley Powell have been my mentors through the years (36 plus years for me) Thank you Mike,Shirley, and Billie Swisherand all my NSH angels that paved the way for us HistoKids Now everyone take a walk and get a breath of fresh air~ Wanda The Original HistoKid ---Original Message--- From: Ingles Claire cing...@uwhealth.org To: Histonet histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Yahoo link Sent: Dec 03 '13 19:05 Old Histologists never die, they're just well fixed... Claire From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Morken, Timothy [timothy.mor...@ucsfmedctr.org] Sent: Tuesday, December 03, 2013 11:22 AM To: 'Shirley A. Powell'; Histonet Subject: [Histonet] RE: Yahoo link Well, Shirley, you are actually an Angel, so nothing will ever stop you!! (from an old Georgia Society hand). Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Tuesday, December 03, 2013 9:18 AM To: Histonet Subject: [Histonet] Yahoo link When I entered the profession I was told the average life expectancy of a histotechs was 20 years from hiring. That scared me but I was already hooked. I have been doing this 51 years, so maybe good laboratory practices can help, in spite of bad ventilation and all those other dangers mentioned. Shirley Powell Antique Histotech -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Tuesday, December 03, 2013 11:40 AM To: Morken, Timothy; Histonet Subject: Re: [Histonet] RE: Yahoo link Just what I was thinking. We will never get enough new students now! With proper training and equipment, we do the job safely! And you can drown in an inch of water. Is that in the MSDS! Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com From: Morken, Timothy timothy.mor...@ucsfmedctr.org To: 'Mike Tighe' mti...@trudeauinstitute.org; histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) histonet@lists.utsouthwestern.edu Sent: Tuesday, December 3, 2013 10:24 AM Subject: [Histonet] RE: Yahoo link Great. Just what we need. Even so, we in the business can take these lists with a grain of K4[Fe(CN) 6] * 3H 2 O since we know that suitable precautions preclude most of the danger. For instance, I'll have to say that the histo lab here is wonderful in that it has such good ventilation (ie., extraction) that there is none of the usual chemical smell - no xylene, alcohol, specials chemicals that often assaults the senses in histology. Vendors that come here are amazed. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Tighe Sent: Tuesday, December 03, 2013 6:31 AM To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Yahoo link Anybody wonder who has the most harzardous job to your health? We're Number One!!! http://finance.yahoo.com/news/the-15-jobs-that-are-most-damaging-to-your-health-155706120.html Mike ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Yahoo link
Couple of studies that I know of. One was sponsored by NSH in the mid-1980's. KH Kilburn came to several NSH Symposiums, and did different tests on people who volunteered to participate. Published findings in the late 1980's that said that histotechs had lower pulmonary function than average population, and decreased memory, equilibrium and dexterity than the general population. In Letters to the Editor, people pointed out statistical flaws (low numbers of participants, for example). I also feel there were flaws, such as testing people after traveling over time zones, who were up late at the parties, and had possibly been drinking the night before. There was no way to measure how much exposure to formaldehyde or xylene people were really exposed to. I didn't participate, but if I though the amount I was being exposed to was medium, someone else being exposed to the same amount might have said low amount and someone else could have said high amount. And the studies would say therefore the low pulmonary exposure was due to histotechs being exposed to formaldehyde. But who could say it was due to that chemical, and not due another chemical, or due to the fact that at the same time, people were smoking in the lab I was working in, which was a small space. Another study somewhat relates - S Khattak in 1999 wrote one on pregnancy outcomes following gestational exposure to organic solvents. They interview women who were pregnant and working with organic solvent, so painters for example, so not histotechs only. They compared them to women of same age, same number of children, similar profession not exposed to organic solvents. They found that if the exposed women were having symptoms (breathing problems, rashes), they had a higher percentage of miscarriages and babies with deformities. If there were no symptoms, they had the same, and even lower, percentages than comparable women giving birth who were not exposed. What histology needs is something like the nursing organization in the US has been doing for over 40 years. Everyone who is a member of the nursing society is sent a survey (I think every year), and asked to have physicals information released from their doctors to the organization (It's voluntary to participate). But they have 40+ years of data from hundred of thousands of women, of all ages. They can mine a wealth of medical data from this. NSH (and ASCP) may not have enough histotechs in their organization to pay for the type of survey needed, to continue on for decades. We would need supervisors and bench techs to participate for decades. And probably have to mail them several formaldehyde and xylene monitors every year, or every couple of years, to collect real numbers of how much people were exposed to. And the surveys were pages and pages long (my mother was a nurse who participated from the beginning, so I've seen them), and asked lots of questions about health and diet and smoking and personal issues, in addition to questions about what types of chemical we work with an how much and how often. And how do we measure ventilation in all these places of work? I hope someone comes up with some studies that can help us figure out if histotechs are being exposed to enough chemicals that could be causing these different diseases in humans. There are animal studies, but not human. And remember, people in general, including histotechs, are living longer. If we live longer, we are more likely to have chronic diseases like diabetes, COPD, and cancer. We need to know which cancers are caused by which levels of formaldehyde or organic solvents, vs., say, breast cancer and prostate cancer just because we are women or men. Peggy A. Wenk, HTL(ASCP)SLS -Original Message- From: Elizabeth Cameron Sent: Wednesday, December 04, 2013 8:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Yahoo link I know the potential for damage to your health is huge in histology, but are there any studies out there that indicate histotechs are less healthy than the average person? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Tuesday, December 03, 2013 7:06 PM To: Histonet Subject: [Histonet] RE: Yahoo link Old Histologists never die, they're just well fixed... Claire From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Morken, Timothy [timothy.mor...@ucsfmedctr.org] Sent: Tuesday, December 03, 2013 11:22 AM To: 'Shirley A. Powell'; Histonet Subject: [Histonet] RE: Yahoo link Well, Shirley, you are actually an Angel, so nothing will ever stop you!! (from an old Georgia Society hand). Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -Original
RE: [Histonet] RE: Yahoo link
I remember participating in the health study in the 80's. Xylene and Formaldehyde levels are monitored in all labs. If our hospitals/research centers would allow us to forward that information on to someone who could compile data it would be a starting place for a health study. I strongly believe this profession can be danger to health. I can name at least 10 histotechs who have died from cancer. Yes, it may have been when they were older but I feel certain it was from all the chemical exposure in histology before safety became a priority. I know others will remember no ventilation, smoking and eating in the lab, no real requirements for gloves, etc. There were also no MSDS information available and we were exposed through lack of knowledge. I believe the lab is much safer today with all of the safety precautions we take and the knowledge we have on chemicals/stains and their toxicity. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.org archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk Sent: Wednesday, December 04, 2013 8:24 AM To: Elizabeth Cameron; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Yahoo link Couple of studies that I know of. One was sponsored by NSH in the mid-1980's. KH Kilburn came to several NSH Symposiums, and did different tests on people who volunteered to participate. Published findings in the late 1980's that said that histotechs had lower pulmonary function than average population, and decreased memory, equilibrium and dexterity than the general population. In Letters to the Editor, people pointed out statistical flaws (low numbers of participants, for example). I also feel there were flaws, such as testing people after traveling over time zones, who were up late at the parties, and had possibly been drinking the night before. There was no way to measure how much exposure to formaldehyde or xylene people were really exposed to. I didn't participate, but if I though the amount I was being exposed to was medium, someone else being exposed to the same amount might have said low amount and someone else could have said high amount. And the studies would say therefore the low pulmonary exposure was due to histotechs being exposed to formaldehyde. But who could say it was due to that chemical, and not due another chemical, or due to the fact that at the same time, people were smoking in the lab I was working in, which was a small space. Another study somewhat relates - S Khattak in 1999 wrote one on pregnancy outcomes following gestational exposure to organic solvents. They interview women who were pregnant and working with organic solvent, so painters for example, so not histotechs only. They compared them to women of same age, same number of children, similar profession not exposed to organic solvents. They found that if the exposed women were having symptoms (breathing problems, rashes), they had a higher percentage of miscarriages and babies with deformities. If there were no symptoms, they had the same, and even lower, percentages than comparable women giving birth who were not exposed. What histology needs is something like the nursing organization in the US has been doing for over 40 years. Everyone who is a member of the nursing society is sent a survey (I think every year), and asked to have physicals information released from their doctors to the organization (It's voluntary to participate). But they have 40+ years of data from hundred of thousands of women, of all ages. They can mine a wealth of medical data from this. NSH (and ASCP) may not have enough histotechs in their organization to pay for the type of survey needed, to continue on for decades. We would need supervisors and bench techs to participate for decades. And probably have to mail them several formaldehyde and xylene monitors every year, or every couple of years, to collect real numbers of how much people were exposed to. And the surveys were pages and pages long (my mother was a nurse who participated from the beginning, so I've seen them), and asked lots of questions about health and diet and smoking and personal issues, in addition to questions about what types of chemical we work with an how much and how often. And how do we measure ventilation in all these places of work? I hope someone comes up with some studies that can help us figure out if histotechs are being exposed to enough chemicals that could be causing these different diseases in humans. There are animal studies, but not human. And remember, people in general, including histotechs, are living longer. If we live longer, we are more likely to have chronic diseases like
Re: [Histonet] RE: Yahoo link
I remember the study and some fairly important people in NSH at the time thinking it was a little overblown. It was not followed up on at the time just a report in JOH and done. I have been in Histology many years (about 50) and like Hazel have seen many of the ones even older than us die of cancer or organ failure. Histology is safer than it has ever been however; I am not sure people really pay enough attention to the dangers with chemicals and tissue that is not well fixed. I remember people smoking in labs, eating (even in the autopsy suite) and having a cup of coffee or soda at the microtome. One of my pathologists told us to clean paraffin off our hands with xylene like he did. We have come a long way just not far enough. Pam Marcum - Original Message - From: Hazel V Horn hor...@archildrens.org To: Lee Peggy Wenk lpw...@sbcglobal.net, Elizabeth Cameron elizabeth.came...@jax.org, histonet@lists.utsouthwestern.edu Sent: Wednesday, December 4, 2013 8:52:13 AM Subject: RE: [Histonet] RE: Yahoo link I remember participating in the health study in the 80's. Xylene and Formaldehyde levels are monitored in all labs. If our hospitals/research centers would allow us to forward that information on to someone who could compile data it would be a starting place for a health study. I strongly believe this profession can be danger to health. I can name at least 10 histotechs who have died from cancer. Yes, it may have been when they were older but I feel certain it was from all the chemical exposure in histology before safety became a priority. I know others will remember no ventilation, smoking and eating in the lab, no real requirements for gloves, etc. There were also no MSDS information available and we were exposed through lack of knowledge. I believe the lab is much safer today with all of the safety precautions we take and the knowledge we have on chemicals/stains and their toxicity. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.org archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk Sent: Wednesday, December 04, 2013 8:24 AM To: Elizabeth Cameron; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Yahoo link Couple of studies that I know of. One was sponsored by NSH in the mid-1980's. KH Kilburn came to several NSH Symposiums, and did different tests on people who volunteered to participate. Published findings in the late 1980's that said that histotechs had lower pulmonary function than average population, and decreased memory, equilibrium and dexterity than the general population. In Letters to the Editor, people pointed out statistical flaws (low numbers of participants, for example). I also feel there were flaws, such as testing people after traveling over time zones, who were up late at the parties, and had possibly been drinking the night before. There was no way to measure how much exposure to formaldehyde or xylene people were really exposed to. I didn't participate, but if I though the amount I was being exposed to was medium, someone else being exposed to the same amount might have said low amount and someone else could have said high amount. And the studies would say therefore the low pulmonary exposure was due to histotechs being exposed to formaldehyde. But who could say it was due to that chemical, and not due another chemical, or due to the fact that at the same time, people were smoking in the lab I was working in, which was a small space. Another study somewhat relates - S Khattak in 1999 wrote one on pregnancy outcomes following gestational exposure to organic solvents. They interview women who were pregnant and working with organic solvent, so painters for example, so not histotechs only. They compared them to women of same age, same number of children, similar profession not exposed to organic solvents. They found that if the exposed women were having symptoms (breathing problems, rashes), they had a higher percentage of miscarriages and babies with deformities. If there were no symptoms, they had the same, and even lower, percentages than comparable women giving birth who were not exposed. What histology needs is something like the nursing organization in the US has been doing for over 40 years. Everyone who is a member of the nursing society is sent a survey (I think every year), and asked to have physicals information released from their doctors to the organization (It's voluntary to participate). But they have 40+ years of data from hundred of thousands of women, of all ages. They can mine a wealth of medical data from this. NSH (and ASCP) may not have enough
[Histonet] RE: Yahoo link
The article doesn't even mention the repetitive motion injuries. IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Yahoo link
I had the same experience, the clinical lab techs asked the supervisor to keep our door closed so they would not have to smell the fumes, just lock us up in it. I was considering calling EPA in to check it out. Thank goodness those days are gone. Use those fume hoods and all the other ppe you can get. :) Shirley Powell -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Wednesday, December 04, 2013 10:57 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Yahoo link I worked in a non-ventilated lab once - back in the 70s - just before the xylene study was released. I knew something was wrong and I knew it was xylene and finally was able to get an exhaust put in the window . I could taste it, smell it, and seemed to cough it up!! And my brain was fuzzier than my usual fuzz! The lab director's office was next to my lab and she moved, because she said she couldn't stand to be next to me! So thankful for good regs now and labs since then that have been very well ventilated. We have come a long way, baby!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, December 04, 2013 9:52 AM To: 'Lee Peggy Wenk'; Elizabeth Cameron; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Yahoo link I remember participating in the health study in the 80's. Xylene and Formaldehyde levels are monitored in all labs. If our hospitals/research centers would allow us to forward that information on to someone who could compile data it would be a starting place for a health study. I strongly believe this profession can be danger to health. I can name at least 10 histotechs who have died from cancer. Yes, it may have been when they were older but I feel certain it was from all the chemical exposure in histology before safety became a priority. I know others will remember no ventilation, smoking and eating in the lab, no real requirements for gloves, etc. There were also no MSDS information available and we were exposed through lack of knowledge. I believe the lab is much safer today with all of the safety precautions we take and the knowledge we have on chemicals/stains and their toxicity. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.org archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk Sent: Wednesday, December 04, 2013 8:24 AM To: Elizabeth Cameron; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Yahoo link Couple of studies that I know of. One was sponsored by NSH in the mid-1980's. KH Kilburn came to several NSH Symposiums, and did different tests on people who volunteered to participate. Published findings in the late 1980's that said that histotechs had lower pulmonary function than average population, and decreased memory, equilibrium and dexterity than the general population. In Letters to the Editor, people pointed out statistical flaws (low numbers of participants, for example). I also feel there were flaws, such as testing people after traveling over time zones, who were up late at the parties, and had possibly been drinking the night before. There was no way to measure how much exposure to formaldehyde or xylene people were really exposed to. I didn't participate, but if I though the amount I was being exposed to was medium, someone else being exposed to the same amount might have said low amount and someone else could have said high amount. And the studies would say therefore the low pulmonary exposure was due to histotechs being exposed to formaldehyde. But who could say it was due to that chemical, and not due another chemical, or due to the fact that at the same time, people were smoking in the lab I was working in, which was a small space. Another study somewhat relates - S Khattak in 1999 wrote one on pregnancy outcomes following gestational exposure to organic solvents. They interview women who were
RE: [Histonet] RE: Yahoo link NO PHI
My current lab the air exchange rate is 58/hr. The good news: you do not smell fumes of any kind. The bad news: we are negative pressure and the unvented bathrooms are just outside our lab. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Wednesday, December 04, 2013 11:42 AM To: Weems, Joyce K.; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Yahoo link I had the same experience, the clinical lab techs asked the supervisor to keep our door closed so they would not have to smell the fumes, just lock us up in it. I was considering calling EPA in to check it out. Thank goodness those days are gone. Use those fume hoods and all the other ppe you can get. :) Shirley Powell -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Wednesday, December 04, 2013 10:57 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Yahoo link I worked in a non-ventilated lab once - back in the 70s - just before the xylene study was released. I knew something was wrong and I knew it was xylene and finally was able to get an exhaust put in the window . I could taste it, smell it, and seemed to cough it up!! And my brain was fuzzier than my usual fuzz! The lab director's office was next to my lab and she moved, because she said she couldn't stand to be next to me! So thankful for good regs now and labs since then that have been very well ventilated. We have come a long way, baby!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, December 04, 2013 9:52 AM To: 'Lee Peggy Wenk'; Elizabeth Cameron; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Yahoo link I remember participating in the health study in the 80's. Xylene and Formaldehyde levels are monitored in all labs. If our hospitals/research centers would allow us to forward that information on to someone who could compile data it would be a starting place for a health study. I strongly believe this profession can be danger to health. I can name at least 10 histotechs who have died from cancer. Yes, it may have been when they were older but I feel certain it was from all the chemical exposure in histology before safety became a priority. I know others will remember no ventilation, smoking and eating in the lab, no real requirements for gloves, etc. There were also no MSDS information available and we were exposed through lack of knowledge. I believe the lab is much safer today with all of the safety precautions we take and the knowledge we have on chemicals/stains and their toxicity. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.org archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk Sent: Wednesday, December 04, 2013 8:24 AM To: Elizabeth Cameron; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Yahoo link Couple of studies that I know of. One was sponsored by NSH in the mid-1980's. KH Kilburn came to several NSH Symposiums, and did different tests on people who volunteered to participate. Published findings in the late 1980's that said that histotechs had lower pulmonary function than average population, and decreased memory, equilibrium and dexterity than the general population. In Letters to the Editor, people pointed out statistical flaws (low numbers of participants, for example). I also feel there were flaws, such as testing people after traveling over time zones, who were up late at the parties, and had possibly been drinking the night before. There was no way to measure how much exposure to formaldehyde or xylene people were really exposed to. I didn't participate, but if I though the amount I was being exposed to was medium, someone else being exposed to the same amount might have said low amount and someone else could have said high
RE: [Histonet] RE: Yahoo link NO PHI
Oh joy!!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: Podawiltz, Thomas [mailto:tpodawi...@lrgh.org] Sent: Wednesday, December 04, 2013 12:17 PM To: Shirley A. Powell; Weems, Joyce K.; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Yahoo link NO PHI My current lab the air exchange rate is 58/hr. The good news: you do not smell fumes of any kind. The bad news: we are negative pressure and the unvented bathrooms are just outside our lab. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Wednesday, December 04, 2013 11:42 AM To: Weems, Joyce K.; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Yahoo link I had the same experience, the clinical lab techs asked the supervisor to keep our door closed so they would not have to smell the fumes, just lock us up in it. I was considering calling EPA in to check it out. Thank goodness those days are gone. Use those fume hoods and all the other ppe you can get. :) Shirley Powell -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Wednesday, December 04, 2013 10:57 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Yahoo link I worked in a non-ventilated lab once - back in the 70s - just before the xylene study was released. I knew something was wrong and I knew it was xylene and finally was able to get an exhaust put in the window . I could taste it, smell it, and seemed to cough it up!! And my brain was fuzzier than my usual fuzz! The lab director's office was next to my lab and she moved, because she said she couldn't stand to be next to me! So thankful for good regs now and labs since then that have been very well ventilated. We have come a long way, baby!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, December 04, 2013 9:52 AM To: 'Lee Peggy Wenk'; Elizabeth Cameron; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Yahoo link I remember participating in the health study in the 80's. Xylene and Formaldehyde levels are monitored in all labs. If our hospitals/research centers would allow us to forward that information on to someone who could compile data it would be a starting place for a health study. I strongly believe this profession can be danger to health. I can name at least 10 histotechs who have died from cancer. Yes, it may have been when they were older but I feel certain it was from all the chemical exposure in histology before safety became a priority. I know others will remember no ventilation, smoking and eating in the lab, no real requirements for gloves, etc. There were also no MSDS information available and we were exposed through lack of knowledge. I believe the lab is much safer today with all of the safety precautions we take and the knowledge we have on chemicals/stains and their toxicity. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.org archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk Sent: Wednesday, December 04, 2013 8:24 AM To: Elizabeth Cameron; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Yahoo link Couple of studies that I know of. One was sponsored by NSH in the mid-1980's. KH Kilburn came to several NSH
[Histonet] Rush bx schedule?
For tissue, what step most contributes to subsequent swelling of tissue when the block is soaked? I'm thinking the 100% ETOH, xylene clearing and paraffin infiltration all contribute, but at what percentage? Here is the current schedule, which apparently was meant to mimic a microwave schedule that was used before the MW quit working. The tissue - kidney and liver bx for the most part, up to 10 per day, is coming out soft and swells a bit when soaked. So sectioning is difficult. We need to have a rapid schedule but need good sections. Obviously we are pushing the limits on this so need a bit of leeway for variation in tissue So, I'm considering which of these steps is the most critical to lengthen. This is on a VIP5 but we can also use a peloris for this processing Formalin 2 min, 50degC (this is not necessary at all, IMHO) 80% ETOH 5 min, 50degC 95%ETOH 5 min, 50degC 100% ETOH 8 min, 50degC Xylene, 9 min 50degC Paraffin, 4 min, Paraffin, 5 min Thanks for your insight! Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.353.1266 (office) tim.mor...@ucsfmedctr.orgmailto:tim.mor...@ucsfmedctr.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] On-slide IHC control workflow?
We are planning our move to using on-slide controls for IHC and I'm wondering how other labs handle the workflow and logistics of matching controls to stain orders. We plan to use a TMA for 80% of our orders. So far we have one TMA that covers most Ab's but the number will probably will be expanded (neuropath-specific, Hempath specific, etc). For those who do this now, how do you handle these tasks: * Do you use TMA or single-tissue controls? Or a mix? * * Cutting all the controls - one or more techs? Part of normal work? Outsource? (especially TMA cutting - inhouse or outsource?) * How do you store the controls? (We plan to cut nearly just-in-time, maybe two days from use). * How do you distribute to the cutters? * How do you Indicate to the cutting techs which control slide to use for a particular stain? (we use over 200 antibodies so need to make as easy as possible without memorization) * How do you prevent the wrong control slide from being used? Anything else we should consider? Thanks for any help!! Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.353.1266 (office) tim.mor...@ucsfmedctr.orgmailto:tim.mor...@ucsfmedctr.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Rush bx schedule?
We do strictly GI biopsies, but if we have a stat case, we have a processing schedule as follows: five minutes in each station of one 70%, two 95%, two 100% and three xylenes, then 10 minutes in each of three paraffins. We have the ASP300 and this has been very successful. I have heard from others they do as little as 2 minutes in each, With a bit longer in the paraffin. Generally our STAT cases are in formalin overnight so we skip that step altogether. Happy Holidays! Cristi Sent from my iPhone On Dec 4, 2013, at 9:31 AM, Morken, Timothy timothy.mor...@ucsfmedctr.org wrote: For tissue, what step most contributes to subsequent swelling of tissue when the block is soaked? I'm thinking the 100% ETOH, xylene clearing and paraffin infiltration all contribute, but at what percentage? Here is the current schedule, which apparently was meant to mimic a microwave schedule that was used before the MW quit working. The tissue - kidney and liver bx for the most part, up to 10 per day, is coming out soft and swells a bit when soaked. So sectioning is difficult. We need to have a rapid schedule but need good sections. Obviously we are pushing the limits on this so need a bit of leeway for variation in tissue So, I'm considering which of these steps is the most critical to lengthen. This is on a VIP5 but we can also use a peloris for this processing Formalin 2 min, 50degC (this is not necessary at all, IMHO) 80% ETOH 5 min, 50degC 95%ETOH 5 min, 50degC 100% ETOH 8 min, 50degC Xylene, 9 min 50degC Paraffin, 4 min, Paraffin, 5 min Thanks for your insight! Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.353.1266 (office) tim.mor...@ucsfmedctr.orgmailto:tim.mor...@ucsfmedctr.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] (no subject)
I second Toni's suggestion. Definitely sounds like the paraffin is still present on the lower sections of the slide. On Wed, Dec 4, 2013 at 10:17 AM, Rathborne, Toni trathbo...@somerset-healthcare.com wrote: Could it be the heating/deparaffinization process? If the upper sections are staining more evenly, then maybe they are free from residual paraffin. Try extending the time in the ovens and/or xylene. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Chapman, Cherie J. Sent: Wednesday, December 04, 2013 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hello all, I am looking for suggestions on issues with our HE stain. I supervised a Veterinary Diagnostic lab for over 27 years and produced top quality sections, HE's, special stains and IHC on a variety of different species in our lab. I am currently working in a Dermatopathology Lab and I am finding inconsistent staining with our HE's. Working with just skin is a challenge all on its own. We have made changes to our staining protocol and just not happy with the end product. What we are observing is inconsistent staining on levels on the same slide. The top section seems to stain more evenly than the middle and bottom sections. I can actually see three different shades of color. The specimens are ribboned sections so I know it is not from thick and thin sections. We have looked at our processing times, microwave vs. oven times, staining reagents, different brands of hematoxylin and eosin, adjustments on staining times, tap water compared to distilled water. Our main processor is the Thermo Scientific STP-420 and our back up is the Sakura VIP V processor.I have been working with Thermo technical support thinking it might be a processing issue. We have a Leica ST5020 Multstainer/CV5030 Robotic Cover slipper we have made several changes that the technical teams has suggested to the reagents and staining time. It's still not the quality that we are looking for. I have had culligan techs out several times to see if it could be something with the water. We can run 100 slides the same day, same reagents and protocol and the HE color is so inconsistent. I would appreciate any suggestions in this matter. Cherie Chapman, BS, HT, HTL (ASCP) Associate Director of Dermatopathology Laboratory University of Missouri Department of Dermatology University Physicians Medical Building Phone: (573) 884-0123 Fax: (573) 884-0834 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (no subject)
Hi Cherie, Since the top section is consistently better, then the possibilities lie in what happens after they are on the slide. As Toni recommends slides that are heated or deparaffinized vertically may have left over paraffin on the lower regions.Maybe you could also try depar with them up on their long edges. Staining slides vertically might also give this appearance if reagent times and rinsing are too short. Staining slides flat would point to slides not level or reagents not evenly dispersed on the slide. I would not rule out thick-thin on ribbons but that is probably not the case since the good section is always at the top. Good luck, Tom T -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, December 04, 2013 10:17 AM To: 'Chapman, Cherie J.'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (no subject) Could it be the heating/deparaffinization process? If the upper sections are staining more evenly, then maybe they are free from residual paraffin. Try extending the time in the ovens and/or xylene. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Chapman, Cherie J. Sent: Wednesday, December 04, 2013 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hello all, I am looking for suggestions on issues with our HE stain. I supervised a Veterinary Diagnostic lab for over 27 years and produced top quality sections, HE's, special stains and IHC on a variety of different species in our lab. I am currently working in a Dermatopathology Lab and I am finding inconsistent staining with our HE's. Working with just skin is a challenge all on its own. We have made changes to our staining protocol and just not happy with the end product. What we are observing is inconsistent staining on levels on the same slide. The top section seems to stain more evenly than the middle and bottom sections. I can actually see three different shades of color. The specimens are ribboned sections so I know it is not from thick and thin sections. We have looked at our processing times, microwave vs. oven times, staining reagents, different brands of hematoxylin and eosin, adjustments on staining times, tap water compared to distilled water. Our main processor is the Thermo Scientific STP-420 and our back up is the Sakura VIP V processor.I have been working with Thermo technical support thinking it might be a processing issue. We have a Leica ST5020 Multstainer/CV5030 Robotic Cover slipper we have made several changes that the technical teams has suggested to the reagents and staining time. It's still not the quality that we are looking for. I have had culligan techs out several times to see if it could be something with the water. We can run 100 slides the same day, same reagents and protocol and the HE color is so inconsistent. I would appreciate any suggestions in this matter. Cherie Chapman, BS, HT, HTL (ASCP) Associate Director of Dermatopathology Laboratory University of Missouri Department of Dermatology University Physicians Medical Building Phone: (573) 884-0123 Fax: (573) 884-0834 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: On-slide IHC control workflow?
This is what we've done when we recently moved to control tissue on every slide for IHC as well as Special Stains. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, December 04, 2013 11:46 AM To: Histonet Subject: [Histonet] On-slide IHC control workflow? We are planning our move to using on-slide controls for IHC and I'm wondering how other labs handle the workflow and logistics of matching controls to stain orders. We plan to use a TMA for 80% of our orders. So far we have one TMA that covers most Ab's but the number will probably will be expanded (neuropath-specific, Hempath specific, etc). For those who do this now, how do you handle these tasks: * Do you use TMA or single-tissue controls? Or a mix? We use single tissues for most and have decreased the number of different tissues being used as controls to a more generic tissue that will cover a wider range of antibodies. * Cutting all the controls - one or more techs? Part of normal work? Outsource? (especially TMA cutting - inhouse or outsource?) Cutting controls is a component of several of the histologists' rotations so every week at least 2 people have this task as part of their rotation. * How do you store the controls? (We plan to cut nearly just-in-time, maybe two days from use). Most are stored at RT; some are stored at - 20 degrees C for those antigens that may lose reactivity at RT * How do you distribute to the cutters? The IHC lab prints control slides and pulls the corresponding blocks to have at the ready for those people on the rotations that include cutting controls...they come pick the slides blocks up when they're ready to cut * How do you Indicate to the cutting techs which control slide to use for a particular stain? (we use over 200 antibodies so need to make as easy as possible without memorization) We have a master list of our antibodies posted where IHC slides are organized, that indicates which control tissue to use for each antibody. * How do you prevent the wrong control slide from being used? People learnif a mistake is made, that person is written up so the importance of paying attention to detail is reinforced. Anything else we should consider? We cut large amounts, i.e. 200 tonsil controls, at a time rather than a couple days before the need for control slides. These are kept in slide files in the area of IHC slide organization where the barcode labels are printed for use on our IHC stainers. The control slides are then labeled along with blank slides for the negative controls and taken to microtomy work stations ; works well for us. Thanks for any help!! Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.353.1266 (office) tim.mor...@ucsfmedctr.orgmailto:tim.mor...@ucsfmedctr.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] On-slide IHC control workflow?
Tim Of course not every method works everywherehere is what I do for myself. I use a mix of MTB blocks with some single tissue when scarcity of tissue makes this sensible. The MTB blocks are based on the IHC panels Make own MTB blocks , feel too expensive to buy for me right now, but might make sense for others. I don't have a micro array tool right now so I do my own improvisingAssign control cutting as a rotated duty. Keep a log of the source block and the number of control slides prepared, (after awhile this helps in cutting down on pulling incorrect controls too)Breast markers, other finicky- refrigerate, (Her 2 - date and use within 6 weeks per CAP) other controls at room temperature in labeled slide boxes. I keep the current control with the slides in the box until it is exhaustedCut more slides for MTB blocks, less of not often requested or single tissue controls- could set stock or par numbers, but haven't needed to do this yetI have controls in the SOP and a table in excel of the current controls, but you can also write it on a copy of the request form beside the name of the AB and tape it up if it changes oftenPeople should be able to pull the correct controls from that, and they are required to check the stain under the 'scope anyhow, so hopefully that would assist in keeping the wrong control from going out I am kind of picky about the record-keeping, but in the end I feel it saves more time than not. Joelle Weaver MAOM, HTL (ASCP) QIHC From: timothy.mor...@ucsfmedctr.org To: histonet@lists.utsouthwestern.edu Date: Wed, 4 Dec 2013 17:45:43 + Subject: [Histonet] On-slide IHC control workflow? We are planning our move to using on-slide controls for IHC and I'm wondering how other labs handle the workflow and logistics of matching controls to stain orders. We plan to use a TMA for 80% of our orders. So far we have one TMA that covers most Ab's but the number will probably will be expanded (neuropath-specific, Hempath specific, etc). For those who do this now, how do you handle these tasks: * Do you use TMA or single-tissue controls? Or a mix? * * Cutting all the controls - one or more techs? Part of normal work? Outsource? (especially TMA cutting - inhouse or outsource?) * How do you store the controls? (We plan to cut nearly just-in-time, maybe two days from use). * How do you distribute to the cutters? * How do you Indicate to the cutting techs which control slide to use for a particular stain? (we use over 200 antibodies so need to make as easy as possible without memorization) * How do you prevent the wrong control slide from being used? Anything else we should consider? Thanks for any help!! Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.353.1266 (office) tim.mor...@ucsfmedctr.orgmailto:tim.mor...@ucsfmedctr.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Thank you for all the suggestions. Nothing more frustrating when you can't point your finger to the exact problem. Thanks again, Cherie Chapman, BS, HT, HTL (ASCP) Associate Director of Dermatopathology Laboratory University of Missouri Department of Dermatology University Physicians Medical Building Phone: (573) 884-0123 Fax: (573) 884-0834 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Helicobacter helmani -- help?
Hi Guys- Does the routine Helicobacter pylori IHC staining generally stain the whole spectrum of helicos? We had a clinician ask specifically about the helmani and we're waiting for a response from the vendor but now I'm curious, too! Thanks in advance and Wednesday-- Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT Tech at a time. 281.852.9457 Office 800.756.3309 Phone Fax ad...@fullstaff.org Sign up for the FREE newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to apn...@fullstaff.org. Please include your name and specialty in the body of the email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CAP survey Question
For a long time I have had our IHC techs print out the run logs from each IHC run on the Benchmark Ultras. The techs then check the slides to make sure the positive and negative controls have worked properly before the slides are sent to the individual pathologists. The pathologists are also supposed to check the controls before looking at the patient slides. Lately in the interest of reducing turnaround time I have been asked why we run the log reports and have a tech look over the controls before they send them to the pathologists since the pathologist will also evaluate the controls. I have been doing this because I wanted the documentation that someone reviewed the controls each time an IHC stain was done. I believe if the pathologists would document someplace that the control slides were reviewed before the patient slides were viewed then I could eliminate the techs looking over the controls also. Problem is how are others documenting that the controls are reviewed? Is this done by the techs, the pathologists, or both? We of course have also used this data for quality assurance of our stains. Thanks for your help. Jim James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] rat's hind paw skin
Hi, First I would like to say thanks a lot to you and also Carl who gave me some helpful tips. Then I would like to ask if you have any practical tips when I want to pick up the skin from perfused animal. I need those special tricks which are not normally mentioned in the articles!, I am going to collect them nest week, kind of stressed since I don’t want to lose the tissues because of clumsy handling!, I you have any tips or link which can help me…?!? Thanks a lot… Cheers, Leila On 03 Dec 2013, at 01:27, Barry Rittman barryritt...@gmail.com wrote: Hi Leila As no one seems to have responded to your email I will add a few items. I used to do some work on wound healing on mouse footpads so circumstances are the same. If you are looking for melanin routine fixation and processing should be finem, melanin will remain. Only thing is that the epidermis is tough and there is not much dermis in this region. Need to make sure that you are careful in cutting sections or the epidermis will separate. Hope this helps if you need any more details please email me. Barry On Mon, Dec 2, 2013 at 3:44 AM, Leila Etemadi leila.etem...@med.lu.sewrote: Hello every body, First I would like to thank you all for your great feedbacks and attention which is such a big improvement in my work, Thank you all! Then, I was wonder if any one has experience on this tissue: rat hind paw skin ( which is hairless). Any inputs will be appreciated, whether is about any specific source where I can find technical information about fixation procedure..., or about sectioning,staining procedure… I am looking for melanin pigments and have no clue if it is possible to see it there or not?!? Thanks in advance, Cheers Leila ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Helicobacter helmani -- help?
The polyclonal antibody from Dako labels H. helmennia in addition to H. pylori. Several of the commercially-available mAbs to H. pylori that I have evaluated do not label H. helmennia. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.car...@hhchealth.org From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Cheryl [tkngfl...@yahoo.com] Sent: Wednesday, December 04, 2013 3:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Helicobacter helmani -- help? Hi Guys- Does the routine Helicobacter pylori IHC staining generally stain the whole spectrum of helicos? We had a clinician ask specifically about the helmani and we're waiting for a response from the vendor but now I'm curious, too! Thanks in advance and Wednesday-- Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT Tech at a time. 281.852.9457 Office 800.756.3309 Phone Fax ad...@fullstaff.org Sign up for the FREE newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to apn...@fullstaff.org. Please include your name and specialty in the body of the email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: CAP survey Question
We also have Ultras Jim. We don't print out the run logs. IHC personnel review the controls before they go to a pathologist in order to catch any problems. The pathologists are supposed to review the controls associated with each case they sign out. Our pathology report has a statement included that the negative and positive controls have stained appropriately. By signing off on each case, the pathologist is attesting to the fact that he has indeed reviewed the controls...we do not police them. So far, this works for us. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Wednesday, December 04, 2013 2:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP survey Question For a long time I have had our IHC techs print out the run logs from each IHC run on the Benchmark Ultras. The techs then check the slides to make sure the positive and negative controls have worked properly before the slides are sent to the individual pathologists. The pathologists are also supposed to check the controls before looking at the patient slides. Lately in the interest of reducing turnaround time I have been asked why we run the log reports and have a tech look over the controls before they send them to the pathologists since the pathologist will also evaluate the controls. I have been doing this because I wanted the documentation that someone reviewed the controls each time an IHC stain was done. I believe if the pathologists would document someplace that the control slides were reviewed before the patient slides were viewed then I could eliminate the techs looking over the controls also. Problem is how are others documenting that the controls are reviewed? Is this done by the techs, the pathologists, or both? We of course have also used this data for quality assurance of our stains. Thanks for your help. Jim James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Helicobacter helmani -- help?
We had same problem at my old job. I don't think they have come up with a separate IHc so our doctor would order an alcian yellow with ihc on children under age of 12 I think but we gave this up cause it's so rare. If you look up on Google under h. Heilmannii, I believe its spelled, it has a lot of papers written on it that might help. Anne -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Wednesday, December 04, 2013 12:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Helicobacter helmani -- help? Hi Guys- Does the routine Helicobacter pylori IHC staining generally stain the whole spectrum of helicos? We had a clinician ask specifically about the helmani and we're waiting for a response from the vendor but now I'm curious, too! Thanks in advance and Wednesday-- Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT Tech at a time. 281.852.9457 Office 800.756.3309 Phone Fax ad...@fullstaff.org Sign up for the FREE newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to apn...@fullstaff.org. Please include your name and specialty in the body of the email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC Buffer (wash)
Hello everyone, Got your Christmas shopping done? (Didn't think so...) Anyway, I'm looking for an IHC PBS/Tween buffer wash concentrate that is comparable to the very expensive Lab Vision PBS/Tween buffer wash. I see some at VWR and FISHER (no shipping charges for our lab) that are 0.5% and 1% Tween 20 washing buffer in PBS. Which percentage is best for IHC on a Lab Vision stainer using a polymer detection kit? As usual, your input is appreciated. Sandy P.S. I know I can make up my own from scratch, just not interested in doing that at the moment... Sandra Cheasty Histology Necropsy Supervisor UW-Madison, School of Veterinary Medicine 608 263-1680 (Office) 608 263-1055 (Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Yahoo link NO PHI
Oh Poop I mean Damm Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Thursday, 5 December 2013 4:17 AM To: Shirley A. Powell; Weems, Joyce K.; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Yahoo link NO PHI My current lab the air exchange rate is 58/hr. The good news: you do not smell fumes of any kind. The bad news: we are negative pressure and the unvented bathrooms are just outside our lab. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Wednesday, December 04, 2013 11:42 AM To: Weems, Joyce K.; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Yahoo link I had the same experience, the clinical lab techs asked the supervisor to keep our door closed so they would not have to smell the fumes, just lock us up in it. I was considering calling EPA in to check it out. Thank goodness those days are gone. Use those fume hoods and all the other ppe you can get. :) Shirley Powell -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Wednesday, December 04, 2013 10:57 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Yahoo link I worked in a non-ventilated lab once - back in the 70s - just before the xylene study was released. I knew something was wrong and I knew it was xylene and finally was able to get an exhaust put in the window . I could taste it, smell it, and seemed to cough it up!! And my brain was fuzzier than my usual fuzz! The lab director's office was next to my lab and she moved, because she said she couldn't stand to be next to me! So thankful for good regs now and labs since then that have been very well ventilated. We have come a long way, baby!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, December 04, 2013 9:52 AM To: 'Lee Peggy Wenk'; Elizabeth Cameron; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Yahoo link I remember participating in the health study in the 80's. Xylene and Formaldehyde levels are monitored in all labs. If our hospitals/research centers would allow us to forward that information on to someone who could compile data it would be a starting place for a health study. I strongly believe this profession can be danger to health. I can name at least 10 histotechs who have died from cancer. Yes, it may have been when they were older but I feel certain it was from all the chemical exposure in histology before safety became a priority. I know others will remember no ventilation, smoking and eating in the lab, no real requirements for gloves, etc. There were also no MSDS information available and we were exposed through lack of knowledge. I believe the lab is much safer today with all of the safety precautions we take and the knowledge we have on chemicals/stains and their toxicity. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.org archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk Sent: Wednesday, December 04, 2013 8:24 AM To: Elizabeth Cameron; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Yahoo link Couple of studies that I know of. One was sponsored by NSH in the mid-1980's. KH Kilburn came to several NSH Symposiums, and did different tests on people who volunteered to participate. Published findings in
[Histonet] RE: Rush bx schedule?
Hi Tim, Try fixing for 45 min at 50oC. I think it possible that the tissue is not adequately fixed. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Thursday, 5 December 2013 4:31 AM To: Histonet Subject: [Histonet] Rush bx schedule? For tissue, what step most contributes to subsequent swelling of tissue when the block is soaked? I'm thinking the 100% ETOH, xylene clearing and paraffin infiltration all contribute, but at what percentage? Here is the current schedule, which apparently was meant to mimic a microwave schedule that was used before the MW quit working. The tissue - kidney and liver bx for the most part, up to 10 per day, is coming out soft and swells a bit when soaked. So sectioning is difficult. We need to have a rapid schedule but need good sections. Obviously we are pushing the limits on this so need a bit of leeway for variation in tissue So, I'm considering which of these steps is the most critical to lengthen. This is on a VIP5 but we can also use a peloris for this processing Formalin 2 min, 50degC (this is not necessary at all, IMHO) 80% ETOH 5 min, 50degC 95%ETOH 5 min, 50degC 100% ETOH 8 min, 50degC Xylene, 9 min 50degC Paraffin, 4 min, Paraffin, 5 min Thanks for your insight! Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.353.1266 (office) tim.mor...@ucsfmedctr.orgmailto:tim.mor...@ucsfmedctr.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: On-slide IHC control workflow?
Unfortunately some controls cannot be confidently used in a TMA eg CD15 - where often Hodgkin's cells are sparse in the block, or CMV or adenovirus - again same reason - sparse positive cells. We sometimes use composite blocks, eg skin and lymph node for S100 and CD1a - (dendritic cells) or (in my past) Prostate adenocarcinoma and salivary gland for prostate specific antigen. We tend to cut our controls and place them near the label end of the slide. We do not heat them but dry them (10minutesbefore placing in a closed labelled box ready for use. The box is labelled with the antigens the control slides are used for. There might be issues with aged pre-cut slides but we have not noticed ant deterioration with the antibodies we use. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Thursday, 5 December 2013 4:46 AM To: Histonet Subject: [Histonet] On-slide IHC control workflow? We are planning our move to using on-slide controls for IHC and I'm wondering how other labs handle the workflow and logistics of matching controls to stain orders. We plan to use a TMA for 80% of our orders. So far we have one TMA that covers most Ab's but the number will probably will be expanded (neuropath-specific, Hempath specific, etc). For those who do this now, how do you handle these tasks: * Do you use TMA or single-tissue controls? Or a mix? * * Cutting all the controls - one or more techs? Part of normal work? Outsource? (especially TMA cutting - inhouse or outsource?) * How do you store the controls? (We plan to cut nearly just-in-time, maybe two days from use). * How do you distribute to the cutters? * How do you Indicate to the cutting techs which control slide to use for a particular stain? (we use over 200 antibodies so need to make as easy as possible without memorization) * How do you prevent the wrong control slide from being used? Anything else we should consider? Thanks for any help!! Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.353.1266 (office) tim.mor...@ucsfmedctr.orgmailto:tim.mor...@ucsfmedctr.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] rat's hind paw skin
If they are perfused, you don't need to worry about stopping them from curling ( they will be fixed/rigid). Get as large a piece as possible: preferably two pieces. (The second piece can then be further fixed for 2hrs before placing into 30% sucrose until the specimen sinks, then snap-freezing. If you don't have a cryostat, forget this.) The piece for Pwax: place in a cassette that you know has holes too small for the specimen to fall thro. I use those plastic mesh inserts: helps to keep tissue flat and safe. They fit nicely into std plastic cassettes. If your current processing protocol is good for your specimens, it will be fine for rat footpad skin. ( if you are going to do HIERyou will have to use charged/coated slides, of course.) You will lose some integrity of the dermis ( collagen will be disrupted), unless you play around with sub-boiling temps. Thanks and.good luck. Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
