RE: [Histonet] marking tiny specimens

[John Kiernan]

Dedication to duty! Ouch! Did you ever run out of eyelashes?  Why not a short 
eyebrow or forearm hair?
JK
= = = 
On 30/01/14, Barbara Tibbs  wrote: 
>  Are you mixing Eosin in the last two alcohols of your processor?   That 
> should work.  If you are dotting the specimens with Eosin before placing in a 
> cassette, the dye will wash out early in the processing.
> 
> Also, I don't know if this helps you but when I had to ink mouse arteries I 
> would glue one of my eyelashes to the end of a wood applicator stick and use 
> the eyelash as a tiny paint brush dipped in India ink.  I would do this under 
> a dissecting microscope.  Maybe you could do that with the eggs?
> 
> Barbara S. Tibbs
> Histology Supervisor
> Accurate Diagnostic Labs
> South Plainfield, NJ
> barbara.ti...@accuratediagnosticlabs.com
> 732-839-3374
> Cell: 610-809-6508
> 
> 
> 
> From: histonet-boun...@lists.utsouthwestern.edu 
>  on behalf of Cheryl Crowder 
> 
> Sent: Thursday, January 30, 2014 5:59 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] marking tiny specimens
> 
> I am processing some extremely small specimens - pin tip size.  These are
> eggs with a protein covering.  I have tried using eosin to color the tissues
> before processing but the color came out before paraffin.  The coating on
> the eggs will not absorb the dye.  Does anyone have a suggestion for dyeing
> or marking these tissues so I can see them better to embed.  Thanks in
> advance,
> Cheryl
> 
> Cheryl Crowder, BA, HTL(ASCP)
> 
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> 
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RE: [Histonet] marking tiny specimens

[Wanda.Smith]

We use a dropper of hematoxylin at the grossing table and it stays on the 
tissue even after processing.

WANDA G. SMITH, HTL(ASCP)HT 
Pathology Supervisor 
TRIDENT MEDICAL CENTER 
9330 Medical Plaza Drive 
Charleston, SC  29406 
843-847-4586 
843-847-4296 fax 

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheryl Crowder
Sent: Thursday, January 30, 2014 3:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] marking tiny specimens

I am processing some extremely small specimens - pin tip size.  These are eggs 
with a protein covering.  I have tried using eosin to color the tissues before 
processing but the color came out before paraffin.  The coating on the eggs 
will not absorb the dye.  Does anyone have a suggestion for dyeing or marking 
these tissues so I can see them better to embed.  Thanks in advance, Cheryl

Cheryl Crowder, BA, HTL(ASCP)

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Re: [Histonet] Digital dictation system

[Sue]

Dragon use it with copath no issues

Sent from my iPhone

> On Jan 30, 2014, at 2:21 PM, "Rodriguez, Arnold"  wrote:
> 
> Which are the best digital dictation systems currently available for 
> grossing? Our LIS is CoPathPlus but they don't have a dictation module.
> 
> Arnold Rodriguez, HT, ASCP
> Histology Supervisor
> Eisenhower Medical Center
> 39000 Bob Hope Drive
> Rancho Mirage, Ca 92270
> Phone (760) 773-2013
> Fax (760) 773-1587
> email: arodrig...@emc.orgmailto:larnodrig...@emc.org>
> 
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[Histonet] RE: Ventana LEAN team

[Bauer, Karen L.]

Hi Amber,

As part of our Vantage implementation process, a workflow consultant initially 
came out to look at our processes.  He was very nice and had a lot of great 
suggestions.  He was never pushy and I had control of what I wanted to change 
or not change.  He basically was there to see how the implementation would go 
according to what we wanted and how things were set up.  Having his overall 
assessment of our workflow and how we could improve steps was very helpful with 
our final implementation of the Vantage system.  

Even if you are not getting the Vantage Specimen Tracking System, the workflow 
assessment will help you in your overall lab design.  Use his/her suggestions 
as you want... It's your lab... We are all unique.  :)

Good luck,

Karen

Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab 
Supervisor | Dermatology | Phone: 715-838-3205 | bauer.ka...@mayo.edu | Mayo 
Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | 
mayoclinichealthsystem.org

 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
Sent: Thursday, January 30, 2014 2:12 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Ventana LEAN team


Has anyone had experience with Ventana's Lean team?  I am correctly designing a 
new lab and they are coming out next tues to look at my workflow.  Just 
wondering what to expect and how much they may change my current layout of my 
future lab :)

Thanks!

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RE: [Histonet] marking tiny specimens

[Truscott, Tom]

Hi Cheryl, If you can, use more eggs. Then process them into a pellet with 
histogel. Upon sectioning, many different cross-sections will be visible in the 
same section. Tom T

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheryl Crowder
Sent: Thursday, January 30, 2014 12:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] marking tiny specimens

I am processing some extremely small specimens - pin tip size.  These are eggs 
with a protein covering.  I have tried using eosin to color the tissues before 
processing but the color came out before paraffin.  The coating on the eggs 
will not absorb the dye.  Does anyone have a suggestion for dyeing or marking 
these tissues so I can see them better to embed.  Thanks in advance, Cheryl

Cheryl Crowder, BA, HTL(ASCP)

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RE: [Histonet] marking tiny specimens

[Elizabeth Chlipala]

Cheryl

So the eggs don't absorb the eosin at all or does the dye come out during 
processing?  We use eosin all of the time for samples like that, but I leave 
them in eosin for about 10 minutes.  Here is what we do, the fixed samples are 
normally submitted in Eppendorf tubes, we remove the formalin and replace with 
eosin (I use Anatechs), let sit for about 10 minutes and then very gently we 
use a plastic disposable pipette (the ones with bulbs) we place the samples in 
a tea bag and process on a very short cycle on the tissue processor (5 -10 
minutes per station) we do this during the day so it's a continuous process.

Liz  

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheryl Crowder
Sent: Thursday, January 30, 2014 1:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] marking tiny specimens

I am processing some extremely small specimens - pin tip size.  These are eggs 
with a protein covering.  I have tried using eosin to color the tissues before 
processing but the color came out before paraffin.  The coating on the eggs 
will not absorb the dye.  Does anyone have a suggestion for dyeing or marking 
these tissues so I can see them better to embed.  Thanks in advance, Cheryl

Cheryl Crowder, BA, HTL(ASCP)

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RE: [Histonet] marking tiny specimens

[Barbara Tibbs]

Are you mixing Eosin in the last two alcohols of your processor?   That should 
work.  If you are dotting the specimens with Eosin before placing in a 
cassette, the dye will wash out early in the processing.

Also, I don't know if this helps you but when I had to ink mouse arteries I 
would glue one of my eyelashes to the end of a wood applicator stick and use 
the eyelash as a tiny paint brush dipped in India ink.  I would do this under a 
dissecting microscope.  Maybe you could do that with the eggs?

Barbara S. Tibbs
Histology Supervisor
Accurate Diagnostic Labs
South Plainfield, NJ
barbara.ti...@accuratediagnosticlabs.com
732-839-3374
Cell: 610-809-6508



From: histonet-boun...@lists.utsouthwestern.edu 
 on behalf of Cheryl Crowder 

Sent: Thursday, January 30, 2014 5:59 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] marking tiny specimens

I am processing some extremely small specimens - pin tip size.  These are
eggs with a protein covering.  I have tried using eosin to color the tissues
before processing but the color came out before paraffin.  The coating on
the eggs will not absorb the dye.  Does anyone have a suggestion for dyeing
or marking these tissues so I can see them better to embed.  Thanks in
advance,
Cheryl

Cheryl Crowder, BA, HTL(ASCP)

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[Histonet] Ventana LEAN team

[Amber McKenzie]

Has anyone had experience with Ventana's Lean team?  I am correctly designing a 
new lab and they are coming out next tues to look at my workflow.  Just 
wondering what to expect and how much they may change my current layout of my 
future lab :)

Thanks!

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[Histonet] marking tiny specimens

[Cheryl Crowder]

I am processing some extremely small specimens - pin tip size.  These are 
eggs with a protein covering.  I have tried using eosin to color the tissues 
before processing but the color came out before paraffin.  The coating on 
the eggs will not absorb the dye.  Does anyone have a suggestion for dyeing 
or marking these tissues so I can see them better to embed.  Thanks in 
advance,
Cheryl

Cheryl Crowder, BA, HTL(ASCP)

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[Histonet] Digital dictation system

[Rodriguez, Arnold]

Which are the best digital dictation systems currently available for grossing? 
Our LIS is CoPathPlus but they don't have a dictation module.

Arnold Rodriguez, HT, ASCP
Histology Supervisor
Eisenhower Medical Center
39000 Bob Hope Drive
Rancho Mirage, Ca 92270
Phone (760) 773-2013
Fax (760) 773-1587
email: arodrig...@emc.orgmailto:larnodrig...@emc.org>

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[Histonet] RELIA Histology Careers Weekly Update 1-30-2014 Are you Ready for Super Bowl Sunday?

[Pam Barker]

Hi Histonetters!!
I hope your week is going great.  
Are you Gearing up for the Super Bowl?
Or the Commercials??
How About the 10th Annual Puppy Bowl?
Or the Inaugural Kitten Bowl?
It's almost like a National Holiday!  And it's this Sunday!!
Looks like these days there is something for everybody on Super Bowl
Sunday!!
 
Before I gear up for the big weekend and whip up a batch of my killer
nachos,
I thought I would drop you a line with my latest most current positions.
Of course all of these positions are permanent and full time.
All of my clients offer excellent compensation, benefits and in most cases
relocation is negotiable.  
These clients are currently interviewing and ready to hire!
Here is a list of my current openings:
HISTOLOGY MANAGEMENT:
Pathology/Histology Supervisor - Cape Cod, MA
Histology Supervisor - Atlanta, GA
Histology Supervisor - Zanesville, OH
HISTOLOGY TECHNICIAN/TECHNOLOGIST
3rd Shift Grossing Histotechnologist - Boston, MA
2nd shift Histology Tech - Boston, MA
Dermpath Histotech - Atlanta, GA
Grossing Tech - Austin, TX
Cytogenetics Tech - Louisville, KY
FISH Tech - Louisville, KY
Cytotech - Lafayette, LA
 
More positions are coming in all of the time so if you are looking let me
know where and what you are looking for and I will keep you posted.
 
REMEMBER. It never hurts to look!*
 
You can reach me toll free at 866-607-3542 until 6 EST and anytime on my
cell phone at 407-353-5070, or shoot me an email at rel...@earthlink.net to
schedule a time to talk.  
 
I really appreciate you taking the time to read this e-mail.
It means a lot to me when you take the time to refer your friends and
coworkers. And to show my appreciation,
I offer you a 500.00 referral fee for anyone you refer to me that I place.
 
Have a Great time on Super Bowl Sunday   Thanks-Pam

Thanks-Pam

Right Place, Right Time, Right Move with RELIA!

Thank You!
 Pam M. Barker
 
Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: rel...@earthlink.net 
www.facebook.com  /PamBarkerRELIA
www.linkedin.com/in/reliasolutions
www.twitter.com/pamatrelia 





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[Histonet] Cryostat Disinfection

[Vickroy, Jim]

In the CAP checklist it states that The cryostat must be4 defrosted and 
decontaminated by wiping all exposed surfaces with a tuberculocidal 
disinfectant.  The cryostat should be at room temperature during 
decontamination unless otherwise specified by the manufacturer.   The newest 
cryostat by Thermofisher uses cold disinfection with a chemical called Sanosil. 
  Am I right thinking that on that cryostat we do not have to disinfect at room 
temperature?

Your thoughts?

James Vickroy BS, HT(ASCP)

Surgical  and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046



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[Histonet] EBV EBNA3A antibody (ab16126, Abcam)

[avistarop]

Hello to everyone!!!

Has anyone used this antibody ?

Sheep polyclonal to EBV EBNA3A antibody (ab16126, Abcam)

This antibody is used on FFPE tissue.

Could I please get a copy of your protocols?

Thank you so much

Aldana Vistarop




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[Histonet] RELIA Histology Careers Weekly Update 1-30-2014 Are you Ready for Super Bowl Sunday?

[Pam Barker]

Hi Histonetters!!
I hope your week is going great.  
Are you Gearing up for the Super Bowl?
Or the Commercials??
How About the 10th Annual Puppy Bowl?
Or the Inaugural Kitten Bowl?
It's almost like a National Holiday!  And it's this Sunday!!
Looks like these days there is something for everybody on Super Bowl
Sunday!!
 
Before I gear up for the big weekend and whip up a batch of my killer
nachos,
I thought I would drop you a line with my latest most current positions.
Of course all of these positions are permanent and full time.
All of my clients offer excellent compensation, benefits and in most cases
relocation is negotiable.  
These clients are currently interviewing and ready to hire!
Here is a list of my current openings:
HISTOLOGY MANAGEMENT:
Pathology/Histology Supervisor - Cape Cod, MA
Histology Supervisor - Atlanta, GA
Histology Supervisor - Zanesville, OH
HISTOLOGY TECHNICIAN/TECHNOLOGIST
3rd Shift Grossing Histotechnologist - Boston, MA
2nd shift Histology Tech - Boston, MA
Dermpath Histotech - Atlanta, GA
Grossing Tech - Austin, TX
Cytogenetics Tech - Louisville, KY
FISH Tech - Louisville, KY
Cytotech - Lafayette, LA
 
More positions are coming in all of the time so if you are looking let me
know where and what you are looking for and I will keep you posted.
 
REMEMBER. It never hurts to look!*
 
You can reach me toll free at 866-607-3542 until 6 EST and anytime on my
cell phone at 407-353-5070, or shoot me an email at rel...@earthlink.net to
schedule a time to talk.  
 
I really appreciate you taking the time to read this e-mail.
It means a lot to me when you take the time to refer your friends and
coworkers. And to show my appreciation,
I offer you a 500.00 referral fee for anyone you refer to me that I place.
 
Have a Great time on Super Bowl Sunday   Thanks-Pam

Thanks-Pam

Right Place, Right Time, Right Move with RELIA!

Thank You!
 Pam M. Barker
 
Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: rel...@earthlink.net 
www.facebook.com  /PamBarkerRELIA
www.linkedin.com/in/reliasolutions
www.twitter.com/pamatrelia 





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RE: [Histonet] Dako's Omnis

[Campbell, Tasha M.]

Are you talking about there IHC stainer, the Link? And there FISH kits
or is there a new instrument that stains FISH?

 
 
 
Tasha Campbell, B.S.,HTL(ASCP)
Frederick Gastroenterology Associates
310 W. 9th St.
Frederick, MD 21701
301-695-6800 ext. 144 (w)
304-685-9307 (c)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joe joe
Sent: Wednesday, January 29, 2014 8:17 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Dako's Omnis






Can anyone share their experiences with me about Dako's new IHC / FISH
instrument?
Thanks in advance!
 
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