[Histonet] RE: Slide Consults
I am ok with them putting the label on the front as long as it does not obscure our label. Especially since our label contains bar codes we use for tracking and storage. __ Lynn M. O'Donnell, CT (ASCP), MHA Technical Specialist, Cytology Danbury Hospital 203-739-6704 Email: lynn.o'donn...@wchn.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bitting, Angela K. Sent: Wednesday, March 19, 2014 17:16 To: Amber McKenzie; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Slide Consults I think it's pretty standard that outside institutions put their accession ID on the front of the consult slides. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Wednesday, March 19, 2014 5:05 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide Consults Would it be rude to ask other hospitals that review my slides to NOT WRITE their accession number on the front of my slides, but instead put their accession number on the back of my slides? Sometimes, I get slides back that have stickers on the front under my accession number or they'll hand write their accession number under mine. I feel like I should scribble out the other institution number so that it doesn't confuse any of us refilling slides. What are your thoughts? Would anyone like to share their slide send out form? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Slide Consults
We do not cover the referring institution label. Will place our label on front if there is clear area; if not, our label is placed on the back of the slide, at the frosted end. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Lynn M. Sent: Thursday, March 20, 2014 8:13 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Slide Consults I am ok with them putting the label on the front as long as it does not obscure our label. Especially since our label contains bar codes we use for tracking and storage. __ Lynn M. O'Donnell, CT (ASCP), MHA Technical Specialist, Cytology Danbury Hospital 203-739-6704 Email: lynn.o'donn...@wchn.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bitting, Angela K. Sent: Wednesday, March 19, 2014 17:16 To: Amber McKenzie; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Slide Consults I think it's pretty standard that outside institutions put their accession ID on the front of the consult slides. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Wednesday, March 19, 2014 5:05 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide Consults Would it be rude to ask other hospitals that review my slides to NOT WRITE their accession number on the front of my slides, but instead put their accession number on the back of my slides? Sometimes, I get slides back that have stickers on the front under my accession number or they'll hand write their accession number under mine. I feel like I should scribble out the other institution number so that it doesn't confuse any of us refilling slides. What are your thoughts? Would anyone like to share their slide send out form? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Lead Histotech opening near Orlando, FL
We are seeking a seasoned Histotechnologist for an Orlando, FL based lab. This is a Lead position on the 3rd shift (overnight) and is a full time/permanent opportunity. Send your resume to resu...@alliedsearchpartners.com to be considered. To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers.php -- Brannon Owens Recruitment Manager Allied Search Partners LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823 http://www.alliedsearchpartners.com T: 888.388.7571 ext. 106 F: 888.388.7572 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Okajima stain control
HI, What would I use for a control for the Okajima stain (hemoglobin). This is my first time doing this stain so any advice would help as well. Thanks! Betsy Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston, TX 77030 832-355-6524 (lab) 832-355-6812 (fax) http://www.texasheart.org Betsy Molinari Senior Histology Research Technician 832-355-6524 | bmolin...@texasheart.orgmailto:bmolin...@texasheart.org | www.texasheart.orghttp://www.texasheart.org 6770 Bertner Ave., MC 1-283, Houston, TX 77030 [Texas Heart Institute]https://secure3.convio.net/thi/site/SPageNavigator/GlobalSiteOptInPage.html[THI News] [THI on Facebook] http://www.facebook.com/Texas.Heart.Institute [THI on Flicker] http://www.flickr.com/photos/texasheart/sets/ [THI on Google] https://plus.google.com/u/0/118043615690351997044/posts [THI on Pinterest] http://pinterest.com/texasheartinst/ [THI on Twitter] http://twitter.com/Texas_Heart [THI on You Tube] http://www.youtube.com/TexasHeartInstitute Confidentiality Notice: This message may be confidential and/or privileged. If you are not the intended recipient you may not review, disseminate or copy this e-mail, its contents and/or any attachments. Please immediately notify the sender If you have received this e-mail in error and delete it from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Melanin Bleaching...
So...I have never done this before. I looked it up in the good ol' bible and found a couple protocols. The pathologist wanted to use the 10% H2O2 procedure because he thought it would be more gentle. So...the slides have been sitting in the 10% solution for 24 hours now. While it is definitely working (slow as a snail...but getting lighter...), can someone please advise on what the end point of this looks like?? Thanks!! =) Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center 12221 North Mopac Expressway Austin, Texas 78758 (512)901-1220 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Slide Consults
it doesn't bother me to have two labels/tracking numbers. I retain theirs so that they keep the liability of identification with the patient blocks and specimen source since I do not have these items to be able to cross check with the slides. The in- house ID is really just for in house processing and tracking, and to be able to use the LIS to capture for QC reports. I leave both visible though and both appear on all work-products. You just ignore the other number when filing and it seems to not be a problem. Joelle Weaver MAOM, HTL (ASCP) QIHC From: becky.garri...@jax.ufl.edu To: Lynn.O'donn...@wchn.org; histonet@lists.utsouthwestern.edu Date: Thu, 20 Mar 2014 13:46:28 + CC: Subject: [Histonet] RE: Slide Consults We do not cover the referring institution label. Will place our label on front if there is clear area; if not, our label is placed on the back of the slide, at the frosted end. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Lynn M. Sent: Thursday, March 20, 2014 8:13 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Slide Consults I am ok with them putting the label on the front as long as it does not obscure our label. Especially since our label contains bar codes we use for tracking and storage. __ Lynn M. O'Donnell, CT (ASCP), MHA Technical Specialist, Cytology Danbury Hospital 203-739-6704 Email: lynn.o'donn...@wchn.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bitting, Angela K. Sent: Wednesday, March 19, 2014 17:16 To: Amber McKenzie; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Slide Consults I think it's pretty standard that outside institutions put their accession ID on the front of the consult slides. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Wednesday, March 19, 2014 5:05 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide Consults Would it be rude to ask other hospitals that review my slides to NOT WRITE their accession number on the front of my slides, but instead put their accession number on the back of my slides? Sometimes, I get slides back that have stickers on the front under my accession number or they'll hand write their accession number under mine. I feel like I should scribble out the other institution number so that it doesn't confuse any of us refilling slides. What are your thoughts? Would anyone like to share their slide send out form? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Oil Red O staining question
Experts! Is there any reason you would not consider formalin fixation followed by cryoprotection/cryosectioning for Oil Red O (or bodipy for that matter) staining of mouse muscle? I ask as many folks seem to have difficulty in the flash freezing aspect of tissue collection and end up with lots of ice crystal damage. While the above method is still subject to freezing artifact it does at least negate the 'rushed collection' part of the process. The literature favors flash frozen tissue but we've done successful staining of liver that has been fixed then cryoprotected prior to staining and things looked fine. Is there an issue with tissue adhering to slides after sectioning or some other reason this method is not preferred? I value your opinion as I am wondering why one would choose one approach over the other. Let's pretend tissue antigenicity isn't a factor - only section quality and lipid droplet staining. Thanks for your comments! David ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Melanin Bleaching...
Hi Sarah, I've bleached out melanin using a 5% Potassium Permangenate solution. It's very quick - about 15 to 30 minutes. Wash thoroughly in running tap water. I've done IHC after bleaching with no damage to the tissue. Hope this helps. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of sarah.dys...@stdavids.com sarah.dys...@stdavids.com Sent: Thursday, March 20, 2014 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Melanin Bleaching... So...I have never done this before. I looked it up in the good ol' bible and found a couple protocols. The pathologist wanted to use the 10% H2O2 procedure because he thought it would be more gentle. So...the slides have been sitting in the 10% solution for 24 hours now. While it is definitely working (slow as a snail...but getting lighter...), can someone please advise on what the end point of this looks like?? Thanks!! =) Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center 12221 North Mopac Expressway Austin, Texas 78758 (512)901-1220 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Oil Red O staining question
I used successfully Oil Red both on flash frozen and formalin-fixed sucrose cryoprotected samples. Don't see any reason why not use formalin fixation. Anatoli Gleiberman, Ph.D. Director of Histopathology Buffalo Biolabs LLC 73 High Street Buffalo, NY 14203 Phone: 716-849-6810x354 e-mail: agleiber...@buffalobiolabs.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of David Burk Sent: Thursday, March 20, 2014 11:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Oil Red O staining question Experts! Is there any reason you would not consider formalin fixation followed by cryoprotection/cryosectioning for Oil Red O (or bodipy for that matter) staining of mouse muscle? I ask as many folks seem to have difficulty in the flash freezing aspect of tissue collection and end up with lots of ice crystal damage. While the above method is still subject to freezing artifact it does at least negate the 'rushed collection' part of the process. The literature favors flash frozen tissue but we've done successful staining of liver that has been fixed then cryoprotected prior to staining and things looked fine. Is there an issue with tissue adhering to slides after sectioning or some other reason this method is not preferred? I value your opinion as I am wondering why one would choose one approach over the other. Let's pretend tissue antigenicity isn't a factor - only section quality and lipid droplet staining. Thanks for your comments! David ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Oil Red O staining question
David That does work, we have done it here on mouse muscle and liver, but there are some problems to fixed and then frozen tissue. The tissue on occasion does not stay on the slide as well once it sectioned, even with plus slides. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of David Burk Sent: Thursday, March 20, 2014 9:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Oil Red O staining question Experts! Is there any reason you would not consider formalin fixation followed by cryoprotection/cryosectioning for Oil Red O (or bodipy for that matter) staining of mouse muscle? I ask as many folks seem to have difficulty in the flash freezing aspect of tissue collection and end up with lots of ice crystal damage. While the above method is still subject to freezing artifact it does at least negate the 'rushed collection' part of the process. The literature favors flash frozen tissue but we've done successful staining of liver that has been fixed then cryoprotected prior to staining and things looked fine. Is there an issue with tissue adhering to slides after sectioning or some other reason this method is not preferred? I value your opinion as I am wondering why one would choose one approach over the other. Let's pretend tissue antigenicity isn't a factor - only section quality and lipid droplet staining. Thanks for your comments! David ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HistoTALK Goes to HISTOPALOOZA 2014
Hello Everyone - HistoTALK (www.HistoTALK.com) was invited to the Georgia Society for Histotechnology's very 1st HISTOPALOOZA. We will be there at Calloway Gardens in Pine Mt., GA from April 25 - 27 (HISTOPALOOZA 2014 dates) interviewing some of our community's most sought after speakers. For everything you ever wanted to know about GSH's way-fun and educational HISTOPALOOZA 2014 in Pine Mt., GA, go to www.HistoSEARCH.com/gsh and check it out. A BIG thank you to President Wanda Simons and the GSH Board for the invite! Yours, Dave HistoTALK ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Oil Red O staining question
We pretty much only used formalin-fixed, frozen tissue for ORO. Just don't process them - the alcohol will dissolve the lipid out. Phil. Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_man...@merck.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Thursday, March 20, 2014 12:30 PM To: David Burk; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Oil Red O staining question David That does work, we have done it here on mouse muscle and liver, but there are some problems to fixed and then frozen tissue. The tissue on occasion does not stay on the slide as well once it sectioned, even with plus slides. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of David Burk Sent: Thursday, March 20, 2014 9:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Oil Red O staining question Experts! Is there any reason you would not consider formalin fixation followed by cryoprotection/cryosectioning for Oil Red O (or bodipy for that matter) staining of mouse muscle? I ask as many folks seem to have difficulty in the flash freezing aspect of tissue collection and end up with lots of ice crystal damage. While the above method is still subject to freezing artifact it does at least negate the 'rushed collection' part of the process. The literature favors flash frozen tissue but we've done successful staining of liver that has been fixed then cryoprotected prior to staining and things looked fine. Is there an issue with tissue adhering to slides after sectioning or some other reason this method is not preferred? I value your opinion as I am wondering why one would choose one approach over the other. Let's pretend tissue antigenicity isn't a factor - only section quality and lipid droplet staining. Thanks for your comments! David ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Slide Consults
Amber McKenzie (where?) asks: Would it be rude to ask other hospitals that review my slides to NOT WRITE their accession number on the front of my slides, but instead put their accession number on the back of my slides? Sometimes, I get slides back that have stickers on the front under my accession number or they'll hand write their accession number under mine. I feel like I should scribble out the other institution number so that it doesn't confuse any of us refilling slides. What are your thoughts? Would anyone like to share their slide send out form? Well, if you put the label on the back of the slide, it becomes very difficult for me to move the slide under the microscope! Surely it's time for us to get Whole Slide Imaging to replace mailing slides around, now that the units have got down to about the price of a good microscope. But at 75 I won't see it! Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fungus control
Hello wise ones! I am having an issue with yeast floating off my lab, agar grown fungus controls. Micro made us a beautiful control 5 months ago. Now all of a sudden we are seeing a significant amount of yeast floating off the control and attaching to the outer edges of the patient tissue. I thought maybe we hadn't achieved complete fixation? Now, as we cut deeper into the control block the yeast cells are breaking away? Any thoughts or suggestions? Thanks, Cheri Cheryl A. Miller HT ASCP cm Histology Supervisor Hygiene Officer Physicians Laboratory, P.C. 4840 F St. Omaha , NE. 68117 402 731 4145 ext. 532 Cell 402 493 0403 Fax 402 731 8653 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Jim Herrick- fluoro labels
Jim, We used to do multiple bone labels at time points in large animals. I did not do the calculations. A veterinarian is the best resource. Also, check publications by Markel.Bouvy.Manley.Zdeblick - all independent researchers that used fluorochome labels and identification using PMMA preparations. Vicki ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHQ Antibody IgD
Hello to everyone!!! Has anyone used this antibody ? Novocastra Mouse Monoclonal Antibody Inmuneglobulin D This antibody is used on FFPE tissue. Could I please get a copy of your protocols? I need to know retrieval epitope time and method. Thank you so much Aldana Vistarop ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] bone marrow and clot and core
Hi All , I was wondering if anyone had any advise on getting clots and cores to stay put? We are using plus slides and giving the slides extra oven time and we are still having issues. So if anyone has some histomagic up their sleeve regarding this issue it would be appreciated. Ginny Miller HT (ASCP) (970)672-6183 Summit Pathology 5802 Wright dr. Loveland co 80538 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Slide Consults
Amber, My lab buys half size colored slide labels and we place them on the bottom of the slide, they are just a little bit bigger than the size of the +'s on plus slides. We have a printer for consult slide labels which when they are received we print these half labels. They do not obscure the labels from the submitting lab, and we can scan our slides for future reference and electronically attach them to the case, with both numbers intact. Amanda Dermatopathology center Washington University Medical School in St. Louis -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Thursday, March 20, 2014 11:51 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Slide Consults Amber McKenzie (where?) asks: Would it be rude to ask other hospitals that review my slides to NOT WRITE their accession number on the front of my slides, but instead put their accession number on the back of my slides? Sometimes, I get slides back that have stickers on the front under my accession number or they'll hand write their accession number under mine. I feel like I should scribble out the other institution number so that it doesn't confuse any of us refilling slides. What are your thoughts? Would anyone like to share their slide send out form? Well, if you put the label on the back of the slide, it becomes very difficult for me to move the slide under the microscope! Surely it's time for us to get Whole Slide Imaging to replace mailing slides around, now that the units have got down to about the price of a good microscope. But at 75 I won't see it! Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fungus Controls (Nathan Sires)
Cheryl, If you are having trouble with the yeast floating down the slide and onto your patient tissue, you can always run the control and the patient tissue on separate slides. Running separate controls is always a good idea when searching for microorganisms (GMS, AFB, or PAS-F stains). Also, I have made my own fungus controls before and it is actually really easy! I also didn’t have any trouble with the fungus floating with this method. All I did was get a sterile urine container, place an orange peel (rind) inside the container, put the lid back on, poke a couple small holes in the top of the lid, and place the container in a window sill somewhere in the laboratory. In a couple of days to a week, you will have some nice fungus (mold) growing on the rind. Once you are done, you can take the rind and cut it into smaller pieces and embed them in separate blocks. This will yield you a whole bunch of fungus controls. : ) Nathan Sires, BA, HT(ASCP) Histology Supervisor McAllen Medical Center 956-632-4255 Histology 512-658-2897 Cell ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cassette/slide writers and embedding center
Hello everyone, I am doing a product survey of sorts and was just wondering which cassette writer, slide writer and embedding center you were using. I don’t need a huge product review but any details worth noting would be good. I am equipment hunting for a lab that will be doing 30-60 clinical samples/month with a potential to increase 4 fold. Any info would be greatly appreciated. Aprill Watanabe, B.S. Laboratory Coordinator The Dorrance Clinical Laboratory at TGen, Histopathology Laboratory Integrated Cancer Genomics Division Macromolecular Analyte Processing Center (MAPC) Translational Genomics Research Institute (TGen) 445 North 5th Street Phoenix, AZ 85004 Office: 602-343-8822 DCL Lab: 602-343-8796 Fax: 602-343-8717 Cell: 602-481-8654 email: awatan...@tgen.org website: www.tgen.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
