RE: [Histonet] On the lighter side...
Also heard Histotechs are good embed!!! LOL -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Monday, August 11, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Old histologists never die, they are just well fixed... Claire From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Michael Ann Jones [mjo...@metropath.com] Sent: Monday, August 11, 2014 9:07 AM To: Edwards, Richard; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 25 years, (what¹s that in micron¹s?²) Bernice, you are too funny!! (lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 8/11/14, 5:17 AM, Edwards, Richard r...@leicester.ac.uk wrote: Sniffed my first formalin and saw first post-mortem November 1965. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Cytospin validation
Good day colleagues Ignoring my previous message what it means: no one did validation for Cytospin !! or no one want to share his information ?? or no one received my message !!?? Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| mailto:j.rowa...@alborglaboratories.com j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | http://www.alborglaboratories.com/ www.alborglaboratories.com From: Jamal [mailto:j.rowa...@alborglaboratories.com] Sent: Monday, August 11, 2014 1:47 PM To: 'histonet@lists.utsouthwestern.edu' Subject: Cytospin validation Hi I need to do validation for my Shandon Cytospin 4, can anyone send me the validation procedure and form. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| mailto:j.rowa...@alborglaboratories.com j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | http://www.alborglaboratories.com/ www.alborglaboratories.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] On the lighter side...
Ha ha!! Good one - Michael Ann On 8/12/14, 12:58 AM, susan.wal...@hcahealthcare.com susan.wal...@hcahealthcare.com wrote: Also heard Histotechs are good embed!!! LOL -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Monday, August 11, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Old histologists never die, they are just well fixed... Claire From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Michael Ann Jones [mjo...@metropath.com] Sent: Monday, August 11, 2014 9:07 AM To: Edwards, Richard; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 25 years, (what¹s that in micron¹s?²) Bernice, you are too funny!! (lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 8/11/14, 5:17 AM, Edwards, Richard r...@leicester.ac.uk wrote: Sniffed my first formalin and saw first post-mortem November 1965. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] looking for TS replacement in Histology due to retirement
Histology Technical Specialist Position in St. Paul, MN Regions Hospital is a Level I Adult and Pediatric trauma Center and teaching hospital serving Minnesota and western Wisconsin for more than 130 years. Regions is a private, non-profit hospital providing outstanding care in women's health, heart, cancer, surgery, orthopaedics, neurosciences, burn, emergency care, and more. Regions Hospital Lab is a state of the art. Regions is a part of the HealthPartners Family of Care. Additional information is available at regionshospital.com Regions Hospital employees enjoy opportunities for personal and professional growth available only at one of the top teaching hospitals in the Twin Cities area. Our dedication to patient care and commitment to a healthy workplace, and Best Care, Best Experience, has allowed us to be recognized by the Minnesota Hospital Association with the Best Minnesota Hospital Workplace Award. This position will oversee the technical component of a work team in the histology laboratory including; implementation of methodologies, supply ordering and inventory management, interpretation of laboratory results for physicians and other hospital staff; performance of laboratory tests in the section assigned; assisting in performance evaluations; assisting in supervision of testing personnel; development and implementation of training and competency programs for histotechnicians and histology students interns, and to perform related duties as assigned. Qualifications: Graduation from an accredited college or university with an Associates (AA) or bachelor's degree in medical technology, histology, biology or related field; plus specialized training in laboratory science. A master's degree in related field may substitute for the above educational and registration requirements. Bachelor's degree preferred. At least four (4) years of experience as a Histotechnician or Histotechnologist or related field with at least two (2) years of experience in the specialty occurring within the last four years. How to Apply Apply online at www.regionshospital.comhttps://careers.peopleclick.com/careerscp/client_healthpartners/regions_external/jobDetails.do?functionName=getJobDetailjobPostId=59124localeCode=en-us Additional Information We are an Equal Opportunity Employer and do not discriminate against applicants due to race, ethnicity, gender, veteran status, or on the basis of disability or any other federal, state or local protected class. This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] protocols
Good afternoon everyone, We are currently looking at or protocols for microtomy. Currently we cut 22 unstained slides for all lung bxs, 10 unstained for all other FNAs, and 6 unstained for liver bxs. Can anyone share their current microtomy protocols? The pathologists are willing to take a look at some change, just would like to know what other institutions are doing? Thanks Debi This e-mail, and any attachments thereto, is intended only for use by the addressee(s) named herein and may contain legally privileged and/or confidential information. If you are not the intended recipient of this e-mail (or the person responsible for delivering this document to the intended recipient), you are hereby notified that any dissemination, distribution, printing or copying of this e-mail, and any attachments thereto, is strictly prohibited. If you have received this e-mail in error, please respond to the individual sending the message and permanently delete the original and any copy of any e-mail and any printout thereof. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: protocols
Good Afternoon, We just pick-up 7-8 sections on treated slides on tiny CT Bxs, liver Bxs and lung bxs. We keep them for a week or two and if they are not used, we throw them away. Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ortiz, Debra Sent: Tuesday, August 12, 2014 1:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] protocols Good afternoon everyone, We are currently looking at or protocols for microtomy. Currently we cut 22 unstained slides for all lung bxs, 10 unstained for all other FNAs, and 6 unstained for liver bxs. Can anyone share their current microtomy protocols? The pathologists are willing to take a look at some change, just would like to know what other institutions are doing? Thanks Debi This e-mail, and any attachments thereto, is intended only for use by the addressee(s) named herein and may contain legally privileged and/or confidential information. If you are not the intended recipient of this e-mail (or the person responsible for delivering this document to the intended recipient), you are hereby notified that any dissemination, distribution, printing or copying of this e-mail, and any attachments thereto, is strictly prohibited. If you have received this e-mail in error, please respond to the individual sending the message and permanently delete the original and any copy of any e-mail and any printout thereof. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: protocols
Wow, that's a lot of unstained! We only cut unstained if requested and we don't cut anything like that for standing protocols. The most we have is 3-5 unstained slides for a the breast protocols and 2 on some liver protocols. The most extensive is Hirschprung's protocol that goes up to 26 slides on a second round, alternating HE and unstained. A few years ago we brought up to our medical director that we had thousands of unstained slides sitting in files so investigated and revamped things. It had been a long-standing practice to always cut 2 extra unstained on ANY immuno order just in case. But that was largely due to very long TAT on IHC staining. Now we have it down to 6-18 hours TAT so if there is a need for more stains they just order more and we cut them as needed. And now we have barcoded slides, including unstained, so they can see in the system exactly how many unstained are left on a particular block. And they can order a stain on the exact unstained slide they want. That alleviates concern that we don't have unstained, or they can't be found. We keep all but FISH and PCR unstained slides in the histo lab. Might this excessive unstained ordering be due to concern about cutting away tissue on initial cutting and recuts? Or is it TAT? Get to the root cause of this and you will find some things to work on in the lab that could alleviate it. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ortiz, Debra Sent: Tuesday, August 12, 2014 10:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] protocols Good afternoon everyone, We are currently looking at or protocols for microtomy. Currently we cut 22 unstained slides for all lung bxs, 10 unstained for all other FNAs, and 6 unstained for liver bxs. Can anyone share their current microtomy protocols? The pathologists are willing to take a look at some change, just would like to know what other institutions are doing? Thanks Debi This e-mail, and any attachments thereto, is intended only for use by the addressee(s) named herein and may contain legally privileged and/or confidential information. If you are not the intended recipient of this e-mail (or the person responsible for delivering this document to the intended recipient), you are hereby notified that any dissemination, distribution, printing or copying of this e-mail, and any attachments thereto, is strictly prohibited. If you have received this e-mail in error, please respond to the individual sending the message and permanently delete the original and any copy of any e-mail and any printout thereof. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Liquid cover glass for HE stained cell culture in 6 well plates; toluidine blue stain for cell cultures
What are people using as a liquid cover glass for stained cells in 6 well culture plates to be view with inverted microscope with having that annoying refractile, funky look to the cells. Cells are grown in 6 well culture plates and with previous cultures, been stained with Hema 3 (hematological stain) and Giemsa of which neither has been successful to see cell membranes and nuclei clearly. Viewing is done with inverted microscope and cells are counted. I know there are some stains that can be viewed left under water with inverted scope. HE obviously cannot be left under water due to eosin washing out of cytoplasm. A possibility is letting HE or T blue stained cells dry, then put a thin layer of permanent mounting media to flow over the cells to fill in spaces, let dry and view with inverted microscope. I think one could do an old bonehead trick we used on surface stained, thicker ground MMA embedded undecalcified bone, flood with immersion oil and put a cover glass on top. Very messy!!! However one could let HE dry after last alcohol rinse, put a layer of immersion oil over the cells, and view with inverted microscope. Anything to fill in the cellular spaces on a confluent sheet of cells but let the oil be the cover glass. Growing cells on a cover glass or on slide with wells/gaskets is not an option. Any suggested stains with protocols would be appreciated too with thoughts here about using Toluidine blue. The recipe for making up the stain, staining method and preferred fixative would be appreciated. You can send information privately if you wish. A challenge to be sure Thanks Gayle Callis HTL/HT/MT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: protocols
hello We collect one unstained for postate biopsies, 5 unstained for lungs (we id each slide with a level number and do 5 unstained), if the biopsy is small the histotech will take come unstained slides and hold them for about a week, we actually place a dot on the block so the techs know there are unstained. If the pathologistrs wants unstained requested upfront they are ordered by the PA's. We found that we were discaring almost a case of unstaained slides a month , since they were plus slides that is about $400 per month. We do not seem to have many issues, although sometimes you just have that case that will haunt you. S Paturzo TJUH ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Weight Loss/Weight Gain Decal _Histonet Digest, Vol 129, Issue 18
Dear all, In the weight loss/gain method how would we fix the optimal endpoint to stop the decal process based on weight gain? Will weighing omit the other ways of judgement such as needle prick, physical examination. In our tox experiments we deal with lot of femurs in one stretch and weighing method looks interesting. Pl. comment. J.Raghul Senior Research Associate Orchid Chemicals and Pharamaceuticals Limited Toxicology, Chennai,India. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: 12 August 2014 22:38 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 129, Issue 18 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: On the lighter side... (Shirley A. Powell) 2. Bcl-2 (Heather Knight) 3. IHC Steam Strips: (Jb) 4. RE: Are Paraffin Blocks Biohazard (Michael LaFriniere) 5. RE: On the lighter side... (Mark Turner) 6. RE: On the lighter side... (Bernice Frederick) 7. Weight Loss/Weight Gain Decal (Wait, Trevor Jordan) 8. Re: Weight Loss/Weight Gain Decal (Jennifer MacDonald) 9. RE: Weight Loss/Weight Gain Decal (Wait, Trevor Jordan) 10. RE: Histonet Digest, Vol 129, Issue 17 (Mayer,Toysha N) 11. RE:On the lighter side... (Mayer,Toysha N) 12. Re: RE:On the lighter side... (Barry Rittman) 13. Re: Weight Loss/Weight Gain decalcification endpoint test (gayle callis) 14. Consult for taking abs from RUO to IVD (Patsy Ruegg) 15. RE: On the lighter side... (susan.wal...@hcahealthcare.com) 16. RE: Cytospin validation (Jamal) 17. Re: On the lighter side... (Michael Ann Jones) 18. looking for TS replacement in Histology due to retirement (Webb, Dorothy L) -- Message: 1 Date: Mon, 11 Aug 2014 13:03:38 -0400 From: Shirley A. Powell powell...@mercer.edu Subject: RE: [Histonet] On the lighter side... To: Ingles Claire cing...@uwhealth.org, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 9BF995BC0E47744E9673A41486E24EE25BFB25FD10@MERCERMAIL.MercerU.local Content-Type: text/plain; charset=iso-8859-1 I agree. :) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Monday, August 11, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Old histologists never die, they are just well fixed... Claire From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Michael Ann Jones [mjo...@metropath.com] Sent: Monday, August 11, 2014 9:07 AM To: Edwards, Richard; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 25 years, (what?s that in micron?s??) Bernice, you are too funny!! (lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 8/11/14, 5:17 AM, Edwards, Richard r...@leicester.ac.uk wrote: Sniffed my first formalin and saw first post-mortem November 1965. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 2 Date: Mon, 11 Aug 2014 13:18:57 -0400 From: Heather Knight heather.l.knig...@gmail.com Subject: [Histonet] Bcl-2 To: histonet@lists.utsouthwestern.edu Message-ID: CABEU4_xVVjqyPu9o8=ONJA__mLNOXAcRmt6pK2oLcSAKp=n...@mail.gmail.com Content-Type: text/plain; charset=UTF-8 Hi everyone- Just wondering if anyone has a working protocol for Bcl-2 in FFPE mouse tissue? If so, can you share both the protocol and the antibody information? We have tried numerous antibodies over the years with very limited success. Thank you for your help!! Best, Heather Knight -- Message: 3 Date: Mon, 11 Aug 2014 10:18:53 -0700 From: Jb craiga...@gmail.com Subject: [Histonet] IHC Steam Strips: To: Histonet@lists.utsouthwestern.edu Message-ID: 375f4ef9-47b6-46cd-b5b1-b511309ef...@gmail.com Content-Type: text/plain; charset=us-ascii Does anyone use IHC steam strips in their decloaking chamber? The question is
