[Histonet] Fixation in concentrated 37% formaldehyde
Dear Histonetters, What would the effect be of fixing tissues samples in concentrated formaldehyde instead of 10% buffered formalin? One of our researchers would like us to prepare some bones for histology staining which have been fixed in 37% formaldehyde by mistake and stored for 4 years. I assume there will be formalin pigment in these samples, so even a HE may not look great, while any enzyme histochemistry or immunohistochemistry is probably not worth trying. I haven't seen the samples yet as they will be arriving from another UK university. Has anyone out there fixed any tissues in concentrated formaldehyde by accident or by design? Thanks, Orla -- ** Ms. Orla Gallagher Bone Analysis Laboratory Mellanby Centre for Bone Research Department of Human Metabolism D Floor Medical School University of Sheffield Beech Hill Road Sheffield S10 2RX UK Website: http://mellanbycentre.dept.shef.ac.uk Tel: 0044114-2713337 (office) 0044114-2713174 (lab) E-Mail:o.m.gallag...@sheffield.ac.uk *STOP*: Do you really need to print this e-mail? *BE GREEN:* Keep it on the screen. http://www.shef.ac.uk/faculty/medicine-dentistry-health/2.9274/emailwording http://www.sheffield.ac.uk/visitors/mapsandtravel ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Fixation in concentrated 37% formaldehyde
Using pure formaldehyde as a fixative is not a good idea (you already know that) and the staining will be affected BUT, on the other hand, IHC will only require stronger HIER (more time and temperature) but other than that I do think the epitopes will be detected. So the effects will be mostly cosmetic.René J. On Wednesday, October 22, 2014 11:04 AM, Orla M Gallagher o.m.gallag...@sheffield.ac.uk wrote: Dear Histonetters, What would the effect be of fixing tissues samples in concentrated formaldehyde instead of 10% buffered formalin? One of our researchers would like us to prepare some bones for histology staining which have been fixed in 37% formaldehyde by mistake and stored for 4 years. I assume there will be formalin pigment in these samples, so even a HE may not look great, while any enzyme histochemistry or immunohistochemistry is probably not worth trying. I haven't seen the samples yet as they will be arriving from another UK university. Has anyone out there fixed any tissues in concentrated formaldehyde by accident or by design? Thanks, Orla -- ** Ms. Orla Gallagher Bone Analysis Laboratory Mellanby Centre for Bone Research Department of Human Metabolism D Floor Medical School University of Sheffield Beech Hill Road Sheffield S10 2RX UK Website: http://mellanbycentre.dept.shef.ac.uk Tel: 0044114-2713337 (office) 0044114-2713174 (lab) E-Mail: o.m.gallag...@sheffield.ac.uk *STOP*: Do you really need to print this e-mail? *BE GREEN:* Keep it on the screen. http://www.shef.ac.uk/faculty/medicine-dentistry-health/2.9274/emailwording http://www.sheffield.ac.uk/visitors/mapsandtravel ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Antibody deposit visualization
One of my clients has been performing experiments with a mouse model of Dengue Virus Infection. They have been treating the mice with monoclonal antibodies (client did not specify what antibody)as a potential treatment for dengue virus infection. They have harvested the kidneys and put them in 10% NBF. They want to know if routine HE will show any antibody deposits. I told him that I would not be able to directly observe the antibody deposit itself, however I might be able to observe the reaction to the antibody deposit in the basement membrane of the glomeruli as a membranous glomerulopathy on an HE. I was wondering if I should do any special stains that might show more than an HE. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Antibody deposit visualization
Depending on the reaction of the glomerulii to the Dengue virus and the stage of such reaction perhaps a PAM (periodic acid methenamine silver) stain could show something.René J. On Wednesday, October 22, 2014 11:31 AM, Rodriguez, Kristofer kristofer.rodrig...@bcm.edu wrote: One of my clients has been performing experiments with a mouse model of Dengue Virus Infection. They have been treating the mice with monoclonal antibodies (client did not specify what antibody)as a potential treatment for dengue virus infection. They have harvested the kidneys and put them in 10% NBF. They want to know if routine HE will show any antibody deposits. I told him that I would not be able to directly observe the antibody deposit itself, however I might be able to observe the reaction to the antibody deposit in the basement membrane of the glomeruli as a membranous glomerulopathy on an HE. I was wondering if I should do any special stains that might show more than an HE. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ATRX
Hi Everyone, Does anyone have ATRX available as a clinical test? I need some that will do the technical only for us. Thanks, Bonnie Bonnie Whitaker AP Operations Director The Ohio State University Wexner Medical Center Department of Pathology N305 Doan Hall 410 West 10th Avenue Columbus, Ohio 43210 614.293.8418 FAX 614.293.2779 Pager: 614.293.7243 ext. 5013 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Linda - drosophila
Have you tried any plastic methods? Feel free to contact me. -- Vicki L. Kalscheur School of Veterinary Medicine Comparative Orthopaedic Research Laboratory 2015 Linden Drive Madison, WI 53706-1100 Phone: 608-262-8534 kalsc...@vetmed.wisc.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Antibody deposit visualization
Hi, may be you can try to label murine immunoglobulins. A mere secondary against murine IgGs could do the trick, depending on which isotype your client is suspecting to form the precipitates. Just an idea, never have tried before. Cheers Julio Rodriguez, Kristofer kristofer.rodrig...@bcm.edu escribió: One of my clients has been performing experiments with a mouse model of Dengue Virus Infection. They have been treating the mice with monoclonal antibodies (client did not specify what antibody)as a potential treatment for dengue virus infection. They have harvested the kidneys and put them in 10% NBF. They want to know if routine HE will show any antibody deposits. I told him that I would not be able to directly observe the antibody deposit itself, however I might be able to observe the reaction to the antibody deposit in the basement membrane of the glomeruli as a membranous glomerulopathy on an HE. I was wondering if I should do any special stains that might show more than an HE. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Unsubscribe me please
Can you please unsubscribe me from your Histo-List. Thanks, Robert Fauck This email or attachment(s) may contain confidential or legally privileged information intended for the sole use of the addressee(s). Any use, redistribution, disclosure, or reproduction of this message, except as intended, is prohibited. If you received this email in error, please notify the sender and remove all copies of the message, including any attachments. Any views or opinions expressed in this email (unless otherwise stated) may not represent those of Capital Coast District Health Board. http://www.ccdhb.org.nz (1C_S1) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Unsubscribe me please
You will need to do that at the website - http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best! j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Fauck [CCDHB] Sent: Wednesday, October 22, 2014 3:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Unsubscribe me please Can you please unsubscribe me from your Histo-List. Thanks, Robert Fauck This email or attachment(s) may contain confidential or legally privileged information intended for the sole use of the addressee(s). Any use, redistribution, disclosure, or reproduction of this message, except as intended, is prohibited. If you received this email in error, please notify the sender and remove all copies of the message, including any attachments. Any views or opinions expressed in this email (unless otherwise stated) may not represent those of Capital Coast District Health Board. http://www.ccdhb.org.nz (1C_S1) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Protocol of MMA Plastic section TRAP staining
Dear Histoneters, Can someone email me your TRAP protocol for MMA section? I have one, but staining is not consistent. No matter I stain for how long, ~ several hours sometimes. There are always variations. I sincerely hope I can get different protocol form you and make change. I remember saw other TRAP protocol which is much longer than mine. The protocol I used was: 1. Dissolve 12mg Naphthol AS-MX phosphate (Sigma, N-5000) in 0.5ml N,N methylformamide (Sigma,D8654). 2. Mix above into 50 ml Sodium tartrate with 0.1M sodium acetate buffer at pH5.0. 3. Mix 30mg Fast Red Violet LB salt (Sigma, F3381) with above solution and filter. Stain slides @37oC for at least one hour. The solution can be use for one month when stored in 4oC. Continue to do counterstain and mount slides. Thank you. Dorothy Hu MGH Endocrine histocore Email: d...@partners.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Antibody deposit visualization
This was years ago be we stained for the virus via IHC. The antibody was supplied by the client. That would seem to me to be the best method to determine deposits. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rodriguez, Kristofer Sent: Wednesday, October 22, 2014 9:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Antibody deposit visualization One of my clients has been performing experiments with a mouse model of Dengue Virus Infection. They have been treating the mice with monoclonal antibodies (client did not specify what antibody)as a potential treatment for dengue virus infection. They have harvested the kidneys and put them in 10% NBF. They want to know if routine HE will show any antibody deposits. I told him that I would not be able to directly observe the antibody deposit itself, however I might be able to observe the reaction to the antibody deposit in the basement membrane of the glomeruli as a membranous glomerulopathy on an HE. I was wondering if I should do any special stains that might show more than an HE. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Processors
Please help me decide on a processor, I am currently inquiring about refurbished processors for a small derm path lab with very low volumes. I have quotes for VIP5 for $24-27k, VIP2000 for $8k, VIP E150 for $14k and a Leica TP1020 type 4 for $17. Which one would you recommend? What's durable and won't break down often? Should I go for the vip2000 compared to vip5 to save money? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Protocol of MMA Plastic section TRAP staining
Hello Dorothy! I will forward you my protocol just as soon as I can get to my computer. On a side note, I no longer Chair the Hard Tissue Committee for the National Society for Histotechnology (NSH) as I have now been elected to the NSH Board of Directors as the Region III Director for the Southeast region. With that said, Sarah Mack is now the new Hard Tissue Committee Chairperson and Kim Simmons is the Education Development Manager for the NSH. I tell you this because Sarah is located in your part of the country and is currently the Histology Core Manager at University of Rochester Medical Center, Center for Musculosketeal Research. She works with bone and will be an good resource for you. I have copied her on this message. Kim Simmons will be helping to organize bone related educational materials and events for the NSH in the next year so she will be another good reference for you to follow. I have copied her to this message as well. I will still be a part of the Hard Tissue Committee in a supporting role. I just wanted you to know your additional options for information related to the histology of bone, biomaterials and medical device implants. Best Regards, Jack Ratliff On Oct 22, 2014, at 3:20 PM, Dorothy Hu abt...@gmail.com wrote: Dear Histoneters, Can someone email me your TRAP protocol for MMA section? I have one, but staining is not consistent. No matter I stain for how long, ~ several hours sometimes. There are always variations. I sincerely hope I can get different protocol form you and make change. I remember saw other TRAP protocol which is much longer than mine. The protocol I used was: 1. Dissolve 12mg Naphthol AS-MX phosphate (Sigma, N-5000) in 0.5ml N,N methylformamide (Sigma,D8654). 2. Mix above into 50 ml Sodium tartrate with 0.1M sodium acetate buffer at pH5.0. 3. Mix 30mg Fast Red Violet LB salt (Sigma, F3381) with above solution and filter. Stain slides @37oC for at least one hour. The solution can be use for one month when stored in 4oC. Continue to do counterstain and mount slides. Thank you. Dorothy Hu MGH Endocrine histocore Email: d...@partners.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
