[Histonet] Auto-Figures

2015-04-09 Thread J. Sedgewick
If anyone in this group has to make figures, or if you know others who do, you 
might be interested in FREE add-ons to Photoshop.  You can get a figure layout 
add-on that will reduce the time it takes to make a figure into seconds.  Other 
add-ons include scripts that ensure you are recording steps in Photoshop for an 
audit trail (good for GLP), and add-ons that include dozens of methods for 
post-processing.

Go to http://www.imagingandanalysis.com.

Best,
Jerry Sedgewick
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[Histonet] Well. Don't I look like a dummy!

2015-04-09 Thread Tom McNemar
Sorry that it took me 3 emails to get the link right.

Licking Memorial Hospital has an opening for a full time certified histology 
tech.
Dayshift position, 7:30am to 4:00pm, Monday through Friday with no weekends or 
holidays.
If interested check us out and apply online at:

http://www.lmhealth.org/

Tom Mc Nemar, HT(ASCP)
Histology Supervisor
(740) 348-4163
Licking Memorial Hospital
1320 West Main Street
Newark, OH  43055



This e-mail, including attachments, is intended for the sole use of the 
individual and/or entity to whom it is addressed, and contains information from 
Licking Memorial Health Systems which is confidential or privileged. If you are 
not the intended recipient, nor authorized to receive for the intended 
recipient, be aware that any disclosure, copying, distribution or use of the 
contents of this e-mail and attachments is prohibited. If you have received 
this in error, please advise the sender by reply e-mail and delete the message 
immediately. You may also contact the LMH Process Improvement Center at 
740-348-4641. E-mail transmissions cannot be guaranteed to be secure or 
error-free as information could be intercepted, corrupted, lost, destroyed, 
arrive late or incomplete, or contain viruses. The sender therefore does not 
accept liability for any errors or omissions in the contents of this message, 
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[Histonet] soften beak

2015-04-09 Thread Rui TAHARA
Hi, 

I am just wondering what you would recommend to soften avian beak for paraffin 
processing. 
I found a protocol that KOH could be used to soften keratin in nail, however, I 
have not been able to find that this method is applicable specific to beak. 
Another concern is if i use KOH for an entire head, does KOH affect (e.g. 
damage) soft tissues?

Thank you in advance, 

rui 

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Re: [Histonet] Maximum size of tissue for paraffin processing

2015-04-09 Thread Rene J Buesa
I am not aware of any such studies [dealing with specimen sizes] but it is a 
known fact since early XXth century that large specimens [such as complete 
brain slices] are known to be processed and yes, they require extended 
dehydrtaion → clearing → infiltration but not much more than usual IF the 
slices are thin enough, and yes, automated tissue processors can be used [I 
have done them for brain and whole prostates].René J.  


 On Thursday, April 9, 2015 2:26 PM, Caroline Miller mi...@3scan.com 
wrote:
   

 Hi there wonderful Histonet people,

Has anyone seen any studies on the maximum size of tissue that can be
paraffin (or resin) processed. I am not talking about the size that can fit
in a tissue cassette, but, for example, entire pig bladder processing.

I realize it would include extended processing time, temp, vacuum,
agitation so imagine that none of these factors were limiting as I shall be
using an automated tissue processor.

I also realize that this is going to be tissue dependent. I was hoping
there were already some studies in this area that I am not finding in both
google and pubmed searches.

thanks, in advance, for any links you can point me to,

yours,
Caroline

-- 
Caroline Miller
Director of Histology
3Scan.com
415 2187297
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 On Thursday, April 9, 2015 2:26 PM, Caroline Miller mi...@3scan.com 
wrote:
   

 Hi there wonderful Histonet people,

Has anyone seen any studies on the maximum size of tissue that can be
paraffin (or resin) processed. I am not talking about the size that can fit
in a tissue cassette, but, for example, entire pig bladder processing.

I realize it would include extended processing time, temp, vacuum,
agitation so imagine that none of these factors were limiting as I shall be
using an automated tissue processor.

I also realize that this is going to be tissue dependent. I was hoping
there were already some studies in this area that I am not finding in both
google and pubmed searches.

thanks, in advance, for any links you can point me to,

yours,
Caroline

-- 
Caroline Miller
Director of Histology
3Scan.com
415 2187297
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[Histonet] Histology position in Newark, Ohio

2015-04-09 Thread Tom McNemar
Sorry.  Not sure why the link didn't work.

www.lmhealth.orghttp://www.lmhealth.org

Tom Mc Nemar, HT(ASCP)
Histology Supervisor
(740) 348-4163
Licking Memorial Hospital
1320 West Main Street
Newark, OH  43055



This e-mail, including attachments, is intended for the sole use of the 
individual and/or entity to whom it is addressed, and contains information from 
Licking Memorial Health Systems which is confidential or privileged. If you are 
not the intended recipient, nor authorized to receive for the intended 
recipient, be aware that any disclosure, copying, distribution or use of the 
contents of this e-mail and attachments is prohibited. If you have received 
this in error, please advise the sender by reply e-mail and delete the message 
immediately. You may also contact the LMH Process Improvement Center at 
740-348-4641. E-mail transmissions cannot be guaranteed to be secure or 
error-free as information could be intercepted, corrupted, lost, destroyed, 
arrive late or incomplete, or contain viruses. The sender therefore does not 
accept liability for any errors or omissions in the contents of this message, 
which arise as a result of e-mail transmission. Thank you.
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[Histonet] Does anyone know why HP35 ultra High profile mocrotome blades no longeer available?

2015-04-09 Thread Van Eyck, Deb
Does anyone know why the HP35 ultra high profile microtome blade is no longer 
available?  It seems like it was very widely used and well liked? Thanks Deb

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[Histonet] Movat Stain Ferric Chloride Solution Color?

2015-04-09 Thread Jerry Ricks
I do a lot of Movat Pentachrome stains.   Every once in a while something goes 
wrong!

I just made a solution of Ferric Chloride  as follows:  Dissolve 12.4 grams 
Ferric Chloride Hexahydrate in 500 ml dH2O.  THen add 5.0 ml of concentrated 
Hydrochloric acid.

What is troubling me is that the solution usually starts of brownish, then 
turns clear yellow after I add the HCl.  This time is stayed brownish and 
didn't turn yellow.

Seems like 15 years ago we did a Movat where someone hadn't added the HCL to 
the solution, it staed brown, and the Weigarts part of the stain didn't work.

Any ideas?  I'm sure I added the HCl.


Jerry L Ricks
Research Scientist
University of Washington
Department of Pathology
  
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[Histonet] Histology position in Newark, Ohio (approximately 30 miles east of Columbus)

2015-04-09 Thread Tom McNemar
Hello all,

Licking Memorial Hospital has an opening for a full time certified histology 
tech.
Dayshift position, 7:30am to 4:00pm, Monday through Friday with no weekends or 
holidays.
If interested check us out and apply online at 
www.lmhealth.orghttp://www.lmhealth.org.


Tom Mc Nemar, HT(ASCP)
Histology Supervisor
(740) 348-4163
Licking Memorial Hospital
1320 West Main Street
Newark, OH  43055



This e-mail, including attachments, is intended for the sole use of the 
individual and/or entity to whom it is addressed, and contains information from 
Licking Memorial Health Systems which is confidential or privileged. If you are 
not the intended recipient, nor authorized to receive for the intended 
recipient, be aware that any disclosure, copying, distribution or use of the 
contents of this e-mail and attachments is prohibited. If you have received 
this in error, please advise the sender by reply e-mail and delete the message 
immediately. You may also contact the LMH Process Improvement Center at 
740-348-4641. E-mail transmissions cannot be guaranteed to be secure or 
error-free as information could be intercepted, corrupted, lost, destroyed, 
arrive late or incomplete, or contain viruses. The sender therefore does not 
accept liability for any errors or omissions in the contents of this message, 
which arise as a result of e-mail transmission. Thank you.
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[Histonet] Maximum size of tissue for paraffin processing

2015-04-09 Thread Caroline Miller
Hi there wonderful Histonet people,

Has anyone seen any studies on the maximum size of tissue that can be
paraffin (or resin) processed. I am not talking about the size that can fit
in a tissue cassette, but, for example, entire pig bladder processing.

I realize it would include extended processing time, temp, vacuum,
agitation so imagine that none of these factors were limiting as I shall be
using an automated tissue processor.

I also realize that this is going to be tissue dependent. I was hoping
there were already some studies in this area that I am not finding in both
google and pubmed searches.

thanks, in advance, for any links you can point me to,

yours,
Caroline

-- 
Caroline Miller
Director of Histology
3Scan.com
415 2187297
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RE: [Histonet] Maximum size of tissue for paraffin processing

2015-04-09 Thread Elizabeth Chlipala
We processed tissues as large as elk and lion vocal cords - 2 cm x 5 cm.  You 
just need to adjust the time accordingly.  Very large samples we would process 
manually through graded alcohols and then the last absolute, xylene and 
paraffin would be run on the tissue processor, we have programs that range from 
6 to 24 hours a station depending upon tissue size.  You can run the entire 
process cycle on a tissue processor its just that we did not want to hang up 
one of the processors for a week on one study.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com

March 10, 2014 is Histotechnology Professionals Day

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Caroline Miller
Sent: Thursday, April 09, 2015 12:26 PM
To: Histonet@Lists. Edu
Subject: [Histonet] Maximum size of tissue for paraffin processing

Hi there wonderful Histonet people,

Has anyone seen any studies on the maximum size of tissue that can be paraffin 
(or resin) processed. I am not talking about the size that can fit in a tissue 
cassette, but, for example, entire pig bladder processing.

I realize it would include extended processing time, temp, vacuum, agitation so 
imagine that none of these factors were limiting as I shall be using an 
automated tissue processor.

I also realize that this is going to be tissue dependent. I was hoping there 
were already some studies in this area that I am not finding in both google and 
pubmed searches.

thanks, in advance, for any links you can point me to,

yours,
Caroline

--
Caroline Miller
Director of Histology
3Scan.com
415 2187297
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[Histonet] Job Opening: Histo Tech/quality assurance analyst position located in Kalamazoo MI

2015-04-09 Thread Jacox, Robert A.
ThermoFisher Scientific currently has an opening for a Histotech focused on 
quality assurance  in Kalamazoo MI. For addition information please send your 
resume or contact Mandy Roschek at (269) 544-5718 or email 
mandy.rosc...@thermofisher.com.

Robert Jacox
Manager, Global Tactical Marketing
Anatomic Pathology

Thermo Fisher Scientific
Tel: 269-544-5651 l Mobile: 269-598-0747
robert.ja...@thermofisher.com l www.thermoscientific.com



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