Re: [Histonet] Histonet Digest, Vol 140, Issue 12
I'm studying for the ASCP Safety Qualification. Looking for advice from an experienced source. Thanks! From: histonet-requ...@lists.utsouthwestern.edu [histonet-requ...@lists.utsouthwestern.edu] Sent: Saturday, July 11, 2015 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 140, Issue 12 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Re: Coverslipping mystery (Caroline Miller) -- Message: 1 Date: Sat, 11 Jul 2015 07:17:30 -0700 From: Caroline Miller mi...@3scan.com To: John Kiernan jkier...@uwo.ca Cc: Adam Boanas a.boa...@epistem.co.uk, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Coverslipping mystery Message-ID: 6c960c7a-bc93-463a-a408-2a94b7918...@3scan.com Content-Type: text/plain; charset=us-ascii I really like DPX, although funnily enough we used cytoseal in my lab in London but always called it the DPX! I think I remember by boss telling me about the bad DPX time. When I moved to the USA the lab I started in had a bottle of DPX and i loved it! I always decant some of the DPX into a 100ml glass bottle, put in a plastic squeeze pipette and then screw a lid on it to stop it drying out (with the pipette still inside) when not in use. Surprisingly the pipette doesn't melt! Which is good because I am a recycle freak and i couldn't stand using a new one every time I mounted something! Yours, mills Caroline Miller (mills) Director of Histology 3Scan, Inc 415-2187297 On Jul 10, 2015, at 10:55 PM, John Kiernan jkier...@uwo.ca wrote: DPX is a polystyrene mounting medium. In principle you can make your own from published recipes. In practice, everyone buys commercial resinous mounting media. In the 1990s we had trouble similar to what you describe. The commercial DPX was cloudy, and not because of alcohol in our xylene. The Canadian supplier acknowledged the bad DPX and urged us to buy Entellan instead. Entellan is a poly(methacrylate) plastic and is an excellent but expensive mounting medium. Another poly(methacrylate) mountant called CytoSeal was less expensive and also came in a squeeze-easy plastic bottle for delivery onto the slide or coverslip. It's now my routine resious mountant. Good DPX returned to the market in the 2000s, but in old-fashioned bottles and not easy to apply to slides or coverslips. John Kiernan = = = On 09/07/15, Adam Boanas a.boa...@epistem.co.uk wrote: Hello, We are having a problem that is developing into a big issue in our lab and I was wondering if anybody could shed any light on it. Our CV5000 coverslipper has recently started introducing microscopic air bubbles onto the slides during coverslipping. We have been told by our engineer that it is a consequence of the age and use of the motor and that sourcing another for an instrument that old (15yrs) will be v difficult. As such, we have been forced to manually coverslip using DPX and a pipette - manually applying the coverslips to the slide, thus mirroring the action of the coverslipper. This is fine at first and for the next few days the slides look great and very clean. However, after about day 4 -5 days post coverslipping, the slides develop an odd appearance down the microscope which looks like very fine `parched earth / crazy paving` all over the slide - including the section. The excess mountant around the edge of the coverslip also has a very faint, cloudy appearance wh! en this occurs. This of course renders the slide un-useable. Does anyone have a clue what this might be down to / how we can stop it? We are struggling for ideas with this one! - this occurs with fresh DPX also. Many thanks Adam Adam Boanas Senior Research Associate Epistem Ltd 48 Grafton Street Manchester, M13 9XX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Subject: Digest Footer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories
Dear Banjo, A reference to the article would be helpful; there must be more to it than one sentence! Formaldehyde has been known for decades to be hazardous, and there are safety regulations in places where it is used. Plenty of old-timers are still alive and well after woking with formaldehyde in the days when there were few or no regulations. I'm one of them. From about 1895 until about 1995 (and perhaps still, in some universities), every medical student spent most of the working day for at least a year with his or her nose and bare hands in a cadaver that had been embalmed in a cocktail containing phenol and formaldehyde. The predominant smell was the phenol, except when dissecting brains, which were fixed and stored in 4% formaldehyde. About 35 years ago, the American Association of Anatomists investigated effects of exposure to embalming chemicals on teachers of anatomy, who are in the dissecting room year after year. The only significant finding was eczema on the hands of some people, long known to be avoidable by wearing rubber gloves. Yes, I too should be able to provide a reference, but this was in the days of paper, which gets thrown out to make room for more paper ... There might be something deep in the archives at http://www.anatomy.org/ Other chemicals used in anatomy, pathology and histology labs also have their dangers; we avoid drinking them, rubbing them into our skin and inhaling their vapours, and we do our best to observe the safety regulations when it comes to getting rid of them. There is no substitute fixative functionally identical to formaldehyde. There are other fixatives, some less hazardous, but they have different effects on staining properties etc. The late Holde Puchtler published papers urging pathologists to use non-aqueous coagulant fixatives for routine fixation of small specimens, with her Carnoy variant methacarn (methanol 60, acetic acid 10, chloroform 30) as the probable best, also good for some modern molecular methods. For this I can provide a few references: Puchtler, H., Waldrop, F.S., Meloan, S.N., Terry, M.S. and Connor, H.M. (1970). Methacarn (methanol-Carnoy) fixation. Practical and theoretical considerations. Histochemie 21:97-116. Cox, M.L., Schray, C.L., Luster, C.N., Stewart, Z.S., Korytko, P.J., Khan, K.N.M., Paulauskis, J.D. and Dunstan, R.W. (2006). Assessment of fixatives, fixation and tissue processing on morphology and RNA integrity. Experimental and Molecular Pathology 80:183-191. Buesa, R.J. (2008). Histology without formalin? Annals of Diagnostic Pathology 12:387-396. Uneyama, C., Shibutani, M., Masutomi, N., Takagi, H. and Hirose, M. (2002). Methacarn fixation for genomic DNA analysis in microdissected paraffin-embedded tissue specimens. Journal of Histochemistry and Cytochemistry 50:1237-1245. Milcheva, R., Janega, P., Celec, P., Russev, R. and Babal, P. (2013). Alcohol based fixatives provide excellent tissue morphology, protein immunoreactivity and RNA integrity in paraffin embedded tissue specimens. Brain Research Protocols 115:279-289. Greer, C.E., Peterson, S.L., Kiviat, N.B. and Manos, M.M. (1991). PCR amplification from paraffin-embedded tissues. American Journal of Clinical Pathology 95:117-124. Tissue processing is extremely simple after non-aqueous coagulant fixation, and most of the stages of a processing machine are not needed. Nuclear chromatin details are much sharper than after formaldehyde. This may not be seen as a blessing by young and middle-aged pathologists. In bygone days the routine fixatives contained mercuric chloride, which gives crisp chromatin and cytoplasmic details. The heterochromatin details probably are artifacts of fixation, but they are useful for identifying cells. John Kiernan Old neuroanatomist and histochemist UWO, London, Canada http://publish.uwo.ca/~jkiernan/ Also Secretary, Biological Stain Commission http://biostain.com = = = On 13/07/15, Adesupo, Adesuyi (Banjo) abades...@nrh-ok.com wrote: Hi, I read this article (National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories) this morning. I wanted to know whether some of you guys out there are using Formaldehyde substitute. Best regards, Banjo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 abades...@nrh-ok.commailto:abades...@nrh-ok.com abades...@nrh-ok.com == CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or
Re: [Histonet] Coverslipping mystery
We have a CV5030 and use the suggested Surgipath micromount. It works well. As has been previously mentioned, you can adjust the amount of mountant dispensed as desired. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: Caroline Miller [mailto:mi...@3scan.com] Sent: Saturday, July 11, 2015 9:18 AM To: John Kiernan Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Coverslipping mystery I really like DPX, although funnily enough we used cytoseal in my lab in London but always called it the DPX! I think I remember by boss telling me about the bad DPX time. When I moved to the USA the lab I started in had a bottle of DPX and i loved it! I always decant some of the DPX into a 100ml glass bottle, put in a plastic squeeze pipette and then screw a lid on it to stop it drying out (with the pipette still inside) when not in use. Surprisingly the pipette doesn't melt! Which is good because I am a recycle freak and i couldn't stand using a new one every time I mounted something! Yours, mills Caroline Miller (mills) Director of Histology 3Scan, Inc 415-2187297 On Jul 10, 2015, at 10:55 PM, John Kiernan jkier...@uwo.ca wrote: DPX is a polystyrene mounting medium. In principle you can make your own from published recipes. In practice, everyone buys commercial resinous mounting media. In the 1990s we had trouble similar to what you describe. The commercial DPX was cloudy, and not because of alcohol in our xylene. The Canadian supplier acknowledged the bad DPX and urged us to buy Entellan instead. Entellan is a poly(methacrylate) plastic and is an excellent but expensive mounting medium. Another poly(methacrylate) mountant called CytoSeal was less expensive and also came in a squeeze-easy plastic bottle for delivery onto the slide or coverslip. It's now my routine resious mountant. Good DPX returned to the market in the 2000s, but in old-fashioned bottles and not easy to apply to slides or coverslips. John Kiernan = = = On 09/07/15, Adam Boanas a.boa...@epistem.co.uk wrote: Hello, We are having a problem that is developing into a big issue in our lab and I was wondering if anybody could shed any light on it. Our CV5000 coverslipper has recently started introducing microscopic air bubbles onto the slides during coverslipping. We have been told by our engineer that it is a consequence of the age and use of the motor and that sourcing another for an instrument that old (15yrs) will be v difficult. As such, we have been forced to manually coverslip using DPX and a pipette - manually applying the coverslips to the slide, thus mirroring the action of the coverslipper. This is fine at first and for the next few days the slides look great and very clean. However, after about day 4 -5 days post coverslipping, the slides develop an odd appearance down the microscope which looks like very fine `parched earth / crazy paving` all over the slide - including the section. The excess mountant around the edge of the coverslip also has a very faint, cloudy appearance wh! en this occurs. This of course renders the slide un-useable. Does anyone have a clue what this might be down to / how we can stop it? We are struggling for ideas with this one! - this occurs with fresh DPX also. Many thanks Adam Adam Boanas Senior Research Associate Epistem Ltd 48 Grafton Street Manchester, M13 9XX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Looking for someone who can do this stain for us
Hello Histonetters, I need your help. I have a doc looking for someone to perform an IHC stain for us. It's a p41/38 moab antibody for HHV-6. If anyone knows of someone doing this stain who also accepts outside cases, I would really appreciate the contact info. Thank you and have a wonderful Monday. SheilaDPPKnoxville, TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Coverslipping mystery
It sounds totally plausible, but just to put a spanner in the works; I have a plastic pipette in my DPX that I leave in there for at least 6 months and I don't get that artifact. We may have different pipettess though. Yours, mills Caroline Miller (mills) Director of Histology 3Scan, Inc 415-2187297 On Jul 13, 2015, at 12:30 AM, Adam Boanas a.boa...@epistem.co.uk wrote: Hello again, Thought I might offer an update on the coverslipping issue as it might be of use in future. I ran a test last week of manual coverslipping using blank charged and un-charged slides and using DPX and Pertex as the mountant. I also used 2 methods of application. 1) Mountant applied using plastic Pasteur pipette 2) Mountant applied using aluminium screw cap tube. Following immersion in xylene for 5 mins the coverslips were applied. From viewing this morning, all slides were clear with the exception of those coverslipped using DPX applied with the Pasteur. In each case, these slides had the 'parched earth' artefact having been left to dry over the weekend. I suspect that the DPX has had a reaction with the plastic of the pipette during application and the artefact is caused by residual `molten` plastic from the pipette that only reveals itself over time. Does this sound plausible? No problem with the pertex and pipettes (which is what I've used for years with no issue) Thanks Adam -Original Message- From: Caroline Miller [mailto:mi...@3scan.com] Sent: 11 July 2015 15:18 To: John Kiernan Cc: Adam Boanas; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Coverslipping mystery I really like DPX, although funnily enough we used cytoseal in my lab in London but always called it the DPX! I think I remember by boss telling me about the bad DPX time. When I moved to the USA the lab I started in had a bottle of DPX and i loved it! I always decant some of the DPX into a 100ml glass bottle, put in a plastic squeeze pipette and then screw a lid on it to stop it drying out (with the pipette still inside) when not in use. Surprisingly the pipette doesn't melt! Which is good because I am a recycle freak and i couldn't stand using a new one every time I mounted something! Yours, mills Caroline Miller (mills) Director of Histology 3Scan, Inc 415-2187297 On Jul 10, 2015, at 10:55 PM, John Kiernan jkier...@uwo.ca wrote: DPX is a polystyrene mounting medium. In principle you can make your own from published recipes. In practice, everyone buys commercial resinous mounting media. In the 1990s we had trouble similar to what you describe. The commercial DPX was cloudy, and not because of alcohol in our xylene. The Canadian supplier acknowledged the bad DPX and urged us to buy Entellan instead. Entellan is a poly(methacrylate) plastic and is an excellent but expensive mounting medium. Another poly(methacrylate) mountant called CytoSeal was less expensive and also came in a squeeze-easy plastic bottle for delivery onto the slide or coverslip. It's now my routine resious mountant. Good DPX returned to the market in the 2000s, but in old-fashioned bottles and not easy to apply to slides or coverslips. John Kiernan = = = On 09/07/15, Adam Boanas a.boa...@epistem.co.uk wrote: Hello, We are having a problem that is developing into a big issue in our lab and I was wondering if anybody could shed any light on it. Our CV5000 coverslipper has recently started introducing microscopic air bubbles onto the slides during coverslipping. We have been told by our engineer that it is a consequence of the age and use of the motor and that sourcing another for an instrument that old (15yrs) will be v difficult. As such, we have been forced to manually coverslip using DPX and a pipette - manually applying the coverslips to the slide, thus mirroring the action of the coverslipper. This is fine at first and for the next few days the slides look great and very clean. However, after about day 4 -5 days post coverslipping, the slides develop an odd appearance down the microscope which looks like very fine `parched earth / crazy paving` all over the slide - including the section. The excess mountant around the edge of the coverslip also has a very faint, cloudy appearance wh! en this occurs. This of course renders the slide un-useable. Does anyone have a clue what this might be down to / how we can stop it? We are struggling for ideas with this one! - this occurs with fresh DPX also. Many thanks Adam Adam Boanas Senior Research Associate Epistem Ltd 48 Grafton Street Manchester, M13 9XX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu
[Histonet] Non Specific Esterase
Hello Histonetters, Does anyone have a protocol for NSE on paraffin sections? Reuel ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Eosin
Rene, As I stated before and maybe I was not entirely clear, we had this problem ( little Eosin in the sections of the first rack of slides on Monday morning) before, but had remedied it by starting to change the Eosin on Monday morning before the first rack of slides was run. He now is stating that the problem of little Eosin in the sections from the first rack on Monday A.M. is starting again. Since the last fix of the problem we have not deviated in any way, procedure or chemical wise. We have used the same lot # in previous weeks for the Eosin as we currently have on the stainer ( I changed the Eosin this morning) , so I don’t think it is an Eosin problem per se. Kari, We do use 95% alcohol before our Eosin at all times. I would think that if the alcohol before the Eosin was the problem, all of my racks would have the “little Eosin in the sections” problem.. not just the first rack. Again any and all suggestions are welcomed!! Thanks again Rene and Kari for replying. Valerie From: Rene J Buesa [mailto:rjbu...@yahoo.com] Sent: Monday, July 13, 2015 11:20 AM To: Hannen, Valerie; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Eosin It is difficult to advise you anything without knowing what is your PT's complaint. What he does complain about? René On Monday, July 13, 2015 11:17 AM, Hannen, Valerie valerie.han...@parrishmed.commailto:valerie.han...@parrishmed.com wrote: Good morning, I once again am dealing with a my picky Pathologist!! About a year ago, he started to complain about not having enough Eosin on his sections( first morning rack on Monday).. I went from changing the Eosin on Friday and stirring it on Monday to totally changing it on Monday...it has all been good until the end of last week. The problem has started up again... little Eosin in the first rack of Monday morning. Any suggestions?? Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.han...@parrishmed.commailto:valerie.han...@parrishmed.commailto:valerie.han...@parrishmed.commailto:valerie.han...@parrishmed.com www.parrishmed.comhttp://www.parrishmed.com == This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you == ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet == This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you == ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories
We are using Excell Plus made by American Master Tech. It is a combination of alcohol, ethylene glycol, and glyoxal. It does not fix quite as fast as formalin but fixes just as well as long as tissue samples are small and not too fatty. We work in veterinary pathology and mostly with necropsy tissues, and try to keep everything 5 to 10 mm maximum thickness that goes into Excell Plus. It definitely won't fix an entire intact brain no matter how long you let it sit so we book the brains into thin slices 24 hours after collection. We have done some limited IHC and DNA extraction from Excell Plus fixed tissue and have not had any issues. We tried another low tox fixative too - Statlab's GTF formalin substitute, which is glyoxal - and it did not fix as well as Excell Plus. GTF fixed CNS tissues especially had a lot of artifact like shrunken neurons. Hope this is helpful. I would also be curious to know how many labs use formalin substitutes because it seems like they have not caught on much (or at all) in the veterinary world. -Nancy Nancy L. Stedman DVM PhD Dipl ACVP Veterinary Pathologist Busch Gardens Tampa nancy.sted...@buschgardens.com -Original Message- From: Adesupo, Adesuyi (Banjo) [mailto:abades...@nrh-ok.com] Sent: Monday, July 13, 2015 12:52 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories Hi, I read this article (National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories) this morning. I wanted to know whether some of you guys out there are using Formaldehyde substitute. Best regards, Banjo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 abades...@nrh-ok.commailto:abades...@nrh-ok.com == CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Eosin
Sometimes these issues are mysterious and in your case are particularly
mysterious because you once solved the problem, only to recently reappear.I
am going to tell you how I used to do it and never had those PT rejections:1-
for both hematoxylin and eosin I kept a log of the number of stained slides and
changes both when I reached 200, regardless of the day of the week or the time
within the day the amount was reached.2- I always used the same brand (Harleco)
3- I never left the staining set over the week. Everything was removed the last
day of work (Saturday) and the containers were rinsed and dried and left upside
down. For the automatic stainer (Sakura) we did the same thing.4- come Monday
morning the whole set was prepared and everything started again.
Now, before I retired all the method was changed and this part probably you
will not attempt: we dewaxed with dishwasher soap (elimination of xylene and
ethanol). The slides went directly to the hematoxylin → bluing →
differentiation → water → eosin → wash water and from then directly to an oven
at 60ºC for 15 minutes (elimination of ethanol and xylene) → coverslip
So, try as I describe in points 1 to 4 or you can even could try eliminating
xylene and ethanol and go green and dry.René
On Monday, July 13, 2015 1:30 PM, Hannen, Valerie
valerie.han...@parrishmed.com wrote:
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{}#yiv6464311563 Rene, As I stated before and maybe I was not entirely clear,
we had this problem ( little Eosin in the sections of the first rack of slides
on Monday morning) before, but had remedied it by starting to change the Eosin
on Monday morning before the first rack of slides was run. He now is stating
that the problem of little Eosin in the sections from the first rack on Monday
A.M. is starting again. Since the last fix of the problem we have not deviated
in any way, procedure or chemical wise. We have used the same lot # in
previous weeks for the Eosin as we currently have on the stainer ( I changed
the Eosin this morning) , so I don’t think it is an Eosin problem per se.
Kari, We do use 95% alcohol before our Eosin at all times. I would think that
if the alcohol before the Eosin was the problem, all of my racks would have
the “little Eosin in the sections” problem.. not just the first rack. Again
any and all suggestions are welcomed!! Thanks again Rene and Kari for
replying. Valerie From: Rene J Buesa [mailto:rjbu...@yahoo.com]
Sent: Monday, July 13, 2015 11:20 AM
To: Hannen, Valerie; Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Eosin It is difficult to advise you anything without
knowing what is your PT's complaint. What he does complain about?
René On Monday, July 13, 2015 11:17 AM, Hannen, Valerie
valerie.han...@parrishmed.com wrote: Good morning,
I once again am dealing with a my picky Pathologist!! About a year ago, he
started to complain about not having enough Eosin on his sections( first
morning rack on Monday).. I went from changing the Eosin on Friday and stirring
it on Monday to totally changing it on Monday...it has all been good until the
end of last week. The problem has started up again... little Eosin in the first
rack of Monday morning. Any suggestions??
Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.han...@parrishmed.commailto:valerie.han...@parrishmed.com
www.parrishmed.com
==
This email is intended solely for the use of the individual to
whom it is addressed and may contain information that is
privileged, confidential or otherwise exempt from disclosure
under applicable law. If the reader of this email is not the
intended recipient or the employee or agent responsible for
delivering the message to the intended recipient, you are
hereby notified that any dissemination, distribution, or
copying of this
[Histonet] Pax8
Is anyone using pax 8 with iview detection on the Ventana Ultra? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories
Hi, I read this article (National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories) this morning. I wanted to know whether some of you guys out there are using Formaldehyde substitute. Best regards, Banjo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 abades...@nrh-ok.commailto:abades...@nrh-ok.com == CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Source for ultra low temperature immersion thermometer
Good Morning Histolanders, Does anyone have a source for an immersion thermometer that can be used to measure the temperature of the isopentane or 2-methylbutane or methylbutane ( you choose) ;-) used for freezing muscle biopsies? I have searched using Dr. Google and cannot find one that goes below -100 degrees C. Thank you in advance. Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UCSD Medical Center 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Source for ultra low temperature immersion thermometer
Paula, we use an Oakton Temp10K with a Type-K thermocouple probe (goes to -250C) . We got it from Fisher scientific https://www.fishersci.com/shop/products/oakton-digi-sense-dual-input-j-k-t-thermocouple-thermometer/p-203772 Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: Paula Sicurello [mailto:pat...@gmail.com] Sent: Monday, July 13, 2015 8:35 AM To: HistoNet Subject: [Histonet] Source for ultra low temperature immersion thermometer Good Morning Histolanders, Does anyone have a source for an immersion thermometer that can be used to measure the temperature of the isopentane or 2-methylbutane or methylbutane ( you choose) ;-) used for freezing muscle biopsies? I have searched using Dr. Google and cannot find one that goes below -100 degrees C. Thank you in advance. Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UCSD Medical Center 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Eosin
Good morning, I once again am dealing with a my picky Pathologist!! About a year ago, he started to complain about not having enough Eosin on his sections( first morning rack on Monday).. I went from changing the Eosin on Friday and stirring it on Monday to totally changing it on Monday...it has all been good until the end of last week. The problem has started up again... little Eosin in the first rack of Monday morning. Any suggestions?? Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.han...@parrishmed.commailto:valerie.han...@parrishmed.com www.parrishmed.com == This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you == ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Eosin
It's always kind of a stab in the dark to diagnose HE problems without a little more information. One thing I would try if you don't feel its an actual Eosin problem is your alcohol prior to the eosin. Slide volume and humidity can dilute your alcohol and cause lighter cytoplasmic staining. The alcohol prior to eosin should be 95% and changed daily if need be. Kari Kienitz HT, (ASCP) Histology Laboratory Gastroenterology-EAST The Oregon Clinic NE 99th Ave Portland, OR 97220 503.935.8311 kkien...@orclinic.com CONFIDENTIALITY WARNING: This e-mail and any attachments are for the exclusive and confidential use of the intended recipient. If you are not the intended recipient, please do not read, distribute or take action in reliance upon this missive. If you have received this in error, please notify the sender immediately by reply e-mail and delete this message and its attachments from your computer system. Thank you From: Hannen, Valerie [valerie.han...@parrishmed.com] Sent: Monday, July 13, 2015 8:05 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin Good morning, I once again am dealing with a my picky Pathologist!! About a year ago, he started to complain about not having enough Eosin on his sections( first morning rack on Monday).. I went from changing the Eosin on Friday and stirring it on Monday to totally changing it on Monday...it has all been good until the end of last week. The problem has started up again... little Eosin in the first rack of Monday morning. Any suggestions?? Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.han...@parrishmed.commailto:valerie.han...@parrishmed.com www.parrishmed.com == This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you == ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Eosin
It is difficult to advise you anything without knowing what is your PT's complaint. What he does complain about? René On Monday, July 13, 2015 11:17 AM, Hannen, Valerie valerie.han...@parrishmed.com wrote: Good morning, I once again am dealing with a my picky Pathologist!! About a year ago, he started to complain about not having enough Eosin on his sections( first morning rack on Monday).. I went from changing the Eosin on Friday and stirring it on Monday to totally changing it on Monday...it has all been good until the end of last week. The problem has started up again... little Eosin in the first rack of Monday morning. Any suggestions?? Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.han...@parrishmed.commailto:valerie.han...@parrishmed.com www.parrishmed.com == This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you == ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CMV antibody clone
Good afternoon Histonet, Just taking a quick poll to find out which CMV clone most folks are running in their labs. Your feedback is much appreciated. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcoo...@chla.usc.edumailto:bcoo...@chla.usc.edu - CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CMV antibody clone
We use DAKO's (an Agilent Technologies Company) product number M0854 (clones DDG9 CCH2). Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -Original Message- From: Cooper, Brian [mailto:bcoo...@chla.usc.edu] Sent: Monday, July 13, 2015 3:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CMV antibody clone Good afternoon Histonet, Just taking a quick poll to find out which CMV clone most folks are running in their labs. Your feedback is much appreciated. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcoo...@chla.usc.edumailto:bcoo...@chla.usc.edu - CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories
The problem in the clinical world is that many clinical trials require formalin fixation, so I would not want to substitute anything that would prevent a patient from being in a trial. It's not new to me that formalin is a carcinogen. I just try to make sure to use PPE and have good ventilation and monitor as necessary. (Unlike when I put my ungloved hands in it and had no ventilation when I started down this path a hundred years ago.) j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: Stedman, Nancy [mailto:nancy.sted...@buschgardens.com] Sent: Monday, July 13, 2015 3:12 PM To: Adesupo, Adesuyi (Banjo); 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories We are using Excell Plus made by American Master Tech. It is a combination of alcohol, ethylene glycol, and glyoxal. It does not fix quite as fast as formalin but fixes just as well as long as tissue samples are small and not too fatty. We work in veterinary pathology and mostly with necropsy tissues, and try to keep everything 5 to 10 mm maximum thickness that goes into Excell Plus. It definitely won't fix an entire intact brain no matter how long you let it sit so we book the brains into thin slices 24 hours after collection. We have done some limited IHC and DNA extraction from Excell Plus fixed tissue and have not had any issues. We tried another low tox fixative too - Statlab's GTF formalin substitute, which is glyoxal - and it did not fix as well as Excell Plus. GTF fixed CNS tissues especially had a lot of artifact like shrunken neurons. Hope this is helpful. I would also be curious to know how many labs use formalin substitutes because it seems like they have not caught on much (or at all) in the veterinary world. -Nancy Nancy L. Stedman DVM PhD Dipl ACVP Veterinary Pathologist Busch Gardens Tampa nancy.sted...@buschgardens.com -Original Message- From: Adesupo, Adesuyi (Banjo) [mailto:abades...@nrh-ok.com] Sent: Monday, July 13, 2015 12:52 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories Hi, I read this article (National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories) this morning. I wanted to know whether some of you guys out there are using Formaldehyde substitute. Best regards, Banjo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 abades...@nrh-ok.commailto:abades...@nrh-ok.com == CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments).
Re: [Histonet] National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories
Like and Ditto to Joyce. Paula Pierce, BS, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Norman, OK 73069 405-759-3953 PH 405-759-7513 FAX www.excaliburpathology.com From: Weems, Joyce K. joyce.we...@emoryhealthcare.org To: Stedman, Nancy nancy.sted...@buschgardens.com; Adesupo, Adesuyi (Banjo) abades...@nrh-ok.com; 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Sent: Monday, July 13, 2015 3:09 PM Subject: Re: [Histonet] National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories The problem in the clinical world is that many clinical trials require formalin fixation, so I would not want to substitute anything that would prevent a patient from being in a trial. It's not new to me that formalin is a carcinogen. I just try to make sure to use PPE and have good ventilation and monitor as necessary. (Unlike when I put my ungloved hands in it and had no ventilation when I started down this path a hundred years ago.) j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: Stedman, Nancy [mailto:nancy.sted...@buschgardens.com] Sent: Monday, July 13, 2015 3:12 PM To: Adesupo, Adesuyi (Banjo); 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories We are using Excell Plus made by American Master Tech. It is a combination of alcohol, ethylene glycol, and glyoxal. It does not fix quite as fast as formalin but fixes just as well as long as tissue samples are small and not too fatty. We work in veterinary pathology and mostly with necropsy tissues, and try to keep everything 5 to 10 mm maximum thickness that goes into Excell Plus. It definitely won't fix an entire intact brain no matter how long you let it sit so we book the brains into thin slices 24 hours after collection. We have done some limited IHC and DNA extraction from Excell Plus fixed tissue and have not had any issues. We tried another low tox fixative too - Statlab's GTF formalin substitute, which is glyoxal - and it did not fix as well as Excell Plus. GTF fixed CNS tissues especially had a lot of artifact like shrunken neurons. Hope this is helpful. I would also be curious to know how many labs use formalin substitutes because it seems like they have not caught on much (or at all) in the veterinary world. -Nancy Nancy L. Stedman DVM PhD Dipl ACVP Veterinary Pathologist Busch Gardens Tampa nancy.sted...@buschgardens.com -Original Message- From: Adesupo, Adesuyi (Banjo) [mailto:abades...@nrh-ok.com] Sent: Monday, July 13, 2015 12:52 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories Hi, I read this article (National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories) this morning. I wanted to know whether some of you guys out there are using Formaldehyde substitute. Best regards, Banjo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 abades...@nrh-ok.commailto:abades...@nrh-ok.com == CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu
[Histonet] HPV
Hello Histonetters: Maybe one of you could lend a hand. I am currently working on FISH validation and I am in the need of HPV positive tissue of the following subtypes: 18, 31, 33, and 51. Does anyone have any extra positive HPV tissue they can spare expressing the above subtypes? I thank you for your help. Regards, Clarence Owens, HT (ASCP)cm, QIHC Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Advice needed on doing the technical component and professional component at two different sites
Currently our dermatologists send their skin biopsies to a private lab. Since we now have our own Histology department we are proposing to do the technical component (grossing and preparation of slides) in house and sending the block and HE slides to the same private company. They call these type of clients professional read only. I understand the differences regarding billing since the CPT codes we are using have a professional component and a technical component. My questions center around what other organizations do for accessioning and how they meet CAP requirements for grossing, etc. Normally we handle all of our other specimens in the normal way of accessioning, grossing (under the indirect supervision of a pathologist that comes to read the slides, processing, preparation of slides, and giving the slides to the pathologist who signs out the surgical report including the gross, and the rest of the surgical report. The dermatology specimens would have to be handled differently. Our dermatologists want the slides read by the private company that only does dermatology specimens. (at least until we hire a dermatopathologist). In the past the derm tissues were sent to the private company and they did all of the process including grossing. Our organization has now decided that it is advantageous for us to do the technical portion in house and then send the slides to be read by the private company because of changes in billing. The private company is perfectly willing to help us make sure they get information they need to diagnose the cases (they have even shown us how they like each type of skin biopsy sectioned and inked. They will help us set up a system for them to get the slides and do the surgical reports. Their report will have our gross description and surgical number, along with a statement that the gross and processing was done elsewhere. They have done this with several other clien ts. I have some unanswered questions since I have never done something like this. Below is a list of my questions. What I really need is someone to tell me how they are handling specimens that they prepare locally and then send the slides to a pathology group to read. 1.Since the local pathologist group does not read these slides, are our grossing techs under the indirect supervision of the pathologists at the private company for these specimens? 2. How do you accession these specimens locally so tracking, slide preparation, and technical component billing can be done, without a local surgical report. 3. If you have to do a local report, who signs the gross report that just has a gross and maybe a statement that the slides will be read at the private company? 4. Are there other CAP considerations I am not thinking of? Am I making this too complicated? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvick...@springfieldclinic.commailto:jvick...@springfieldclinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CMV antibody clone
Thank you to everyone who replied to this post. In case you're wondering, I got like 8 responses today, and everyone who responded uses CMV (DDG9/CCH2) from either Cell Marque or Dako. You guys are the best! Thanks, Brian -Original Message- From: Cooper, Brian [mailto:bcoo...@chla.usc.edu] Sent: Monday, July 13, 2015 12:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CMV antibody clone Good afternoon Histonet, Just taking a quick poll to find out which CMV clone most folks are running in their labs. Your feedback is much appreciated. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcoo...@chla.usc.edumailto:bcoo...@chla.usc.edu - CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Coverslipping mystery
Hello again, Thought I might offer an update on the coverslipping issue as it might be of use in future. I ran a test last week of manual coverslipping using blank charged and un-charged slides and using DPX and Pertex as the mountant. I also used 2 methods of application. 1) Mountant applied using plastic Pasteur pipette 2) Mountant applied using aluminium screw cap tube. Following immersion in xylene for 5 mins the coverslips were applied. From viewing this morning, all slides were clear with the exception of those coverslipped using DPX applied with the Pasteur. In each case, these slides had the 'parched earth' artefact having been left to dry over the weekend. I suspect that the DPX has had a reaction with the plastic of the pipette during application and the artefact is caused by residual `molten` plastic from the pipette that only reveals itself over time. Does this sound plausible? No problem with the pertex and pipettes (which is what I've used for years with no issue) Thanks Adam -Original Message- From: Caroline Miller [mailto:mi...@3scan.com] Sent: 11 July 2015 15:18 To: John Kiernan Cc: Adam Boanas; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Coverslipping mystery I really like DPX, although funnily enough we used cytoseal in my lab in London but always called it the DPX! I think I remember by boss telling me about the bad DPX time. When I moved to the USA the lab I started in had a bottle of DPX and i loved it! I always decant some of the DPX into a 100ml glass bottle, put in a plastic squeeze pipette and then screw a lid on it to stop it drying out (with the pipette still inside) when not in use. Surprisingly the pipette doesn't melt! Which is good because I am a recycle freak and i couldn't stand using a new one every time I mounted something! Yours, mills Caroline Miller (mills) Director of Histology 3Scan, Inc 415-2187297 On Jul 10, 2015, at 10:55 PM, John Kiernan jkier...@uwo.ca wrote: DPX is a polystyrene mounting medium. In principle you can make your own from published recipes. In practice, everyone buys commercial resinous mounting media. In the 1990s we had trouble similar to what you describe. The commercial DPX was cloudy, and not because of alcohol in our xylene. The Canadian supplier acknowledged the bad DPX and urged us to buy Entellan instead. Entellan is a poly(methacrylate) plastic and is an excellent but expensive mounting medium. Another poly(methacrylate) mountant called CytoSeal was less expensive and also came in a squeeze-easy plastic bottle for delivery onto the slide or coverslip. It's now my routine resious mountant. Good DPX returned to the market in the 2000s, but in old-fashioned bottles and not easy to apply to slides or coverslips. John Kiernan = = = On 09/07/15, Adam Boanas a.boa...@epistem.co.uk wrote: Hello, We are having a problem that is developing into a big issue in our lab and I was wondering if anybody could shed any light on it. Our CV5000 coverslipper has recently started introducing microscopic air bubbles onto the slides during coverslipping. We have been told by our engineer that it is a consequence of the age and use of the motor and that sourcing another for an instrument that old (15yrs) will be v difficult. As such, we have been forced to manually coverslip using DPX and a pipette - manually applying the coverslips to the slide, thus mirroring the action of the coverslipper. This is fine at first and for the next few days the slides look great and very clean. However, after about day 4 -5 days post coverslipping, the slides develop an odd appearance down the microscope which looks like very fine `parched earth / crazy paving` all over the slide - including the section. The excess mountant around the edge of the coverslip also has a very faint, cloudy appearance wh! en this occurs. This of course renders the slide un-useable. Does anyone have a clue what this might be down to / how we can stop it? We are struggling for ideas with this one! - this occurs with fresh DPX also. Many thanks Adam Adam Boanas Senior Research Associate Epistem Ltd 48 Grafton Street Manchester, M13 9XX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
