Re: [Histonet] AFB contaminant - OR - Monkish Preparation
I slways filtered my air (5um) and my water (0.22um). Only tap water rinses for Hemnatoxylin, Schiff's and other rinses were not rinsed. All of my MolBio and Immuno- stuff was done with filtered (clean?) water at least. The rule is: be Monkish at the bench and as messy as you like in your bedroom (if you are a male and only until you are married), Cheers, Fred Inside your water supply, lurking behind the water fawcet, is a pipe who's surface is lined with a BIO-film. Further horrors await the scientist who fixes and studies the washer in the fawcet whose seal is the better for the 'FILM-m.' Evolution provides variation, and every new flower that grows provides a new isolated environment for speciation. My alimentary biome is unique as we are all unique. Yukky, but true. Frederick C Monson, PhD Center for Microanalysis and Imaging, Research and Training (CMIRT) West Chester University of Pannsylvania West Chester, PA, 1938 610-738-0437 fmon...@wcupa.edu From: Abbott, Tanya tanyaabb...@catholichealth.net Sent: Thursday, July 9, 2015 3:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AFB contaminant Has anyone ever had a problem with a possible contaminant in their AFB hand stain? If so, how did you deal with it? Any suggestions? Ours actually looked like an AFB like organism, which we later determined to be negative. Thanks! Tanya G. Abbott Manager Technologist Histology/Cytology St Joseph Medical Center (phone) 610-378-2635 This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Masson's on Frozens.
Question: When we stain and HE on frozen we fix in 95% prior to staining. As I never in 30 years have done a Masson's Trichrome on a frozen section I ask : do I need to fix and then mordant as usual or go straight to the Bouin's and continue as normal? Do I need to mordant at all? Tissue is rabbit liver. BErnice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edumailto:b-freder...@northwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Test
Test Cynthia Bulmer HT(ASCP),QIHC IHC Supervisor, CTPL Waco, TX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC negative controls
Happy Friday everyone! We currently have a Ventana Ultra. I am wondering what everyone is doing/running for a negative reagent control and/or a negative tissue control? Thanks in advance! Tanya Tanya G. Abbott Manager Technologist Histology/Cytology St Joseph Medical Center (phone) 610-378-2635 This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC negative controls
We run patient tissue as a negative and we use diluent as the reagent negative. TH Message: 7 Date: Fri, 17 Jul 2015 14:48:17 + From: Abbott, Tanya tanyaabb...@catholichealth.net To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC negative controls Message-ID: 852f7d2c14fb464d80e182b15db138af6b7ec...@chiex005.chi.catholichealth.net Content-Type: text/plain; charset=us-ascii Happy Friday everyone! We currently have a Ventana Ultra. I am wondering what everyone is doing/running for a negative reagent control and/or a negative tissue control? Thanks in advance! Tanya Tanya G. Abbott Manager Technologist Histology/Cytology St Joseph Medical Center (phone) 610-378-2635 This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Waste disposal of histology chemicals in Texas Cameron County
Hello histo people, I am trying to get a new small histology lab together here and I'm new to the state of Texas and to Cameron County. I lived in Virgina before for a private company and a couple community hospitals before moving here. In Virgina we neutralized our formalin waste and once it was neurtalized we poured it down the sink with lots of water. Can we do that in Texas (Cameron county)?? Where could I find info on histology wastes? I will have clear-rite, alcohols, small quantities of xylene, and some special stains probably. Can stuff like paraffin go in the regular trash and a waste disposal company take all the rest like alcohol, clear-rite, and special stain waste? Wayne Gill, HTL(ASCP)CM ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
