Re: [Histonet] Ventana H.Pylori antibody
We also are having the same problem with the Ventana H.Pylori antibody on our Ultras, I have tried the CellMarque product in the past and as mentioned still not ideal. Will try the Biocare product and see if the results improve for us also. Marcia M White Pathology Manager Memorial Regional Hospital 954-265-5371-office 954-967-7627-fax mwh...@mhs.net -Original Message- From: Jeffrey Robinson [mailto:jrobin...@pathology-associates.com] Sent: Thursday, July 30, 2015 1:06 PM To: Cates, Julia; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana H.Pylori antibody Hi Julia- I have had a lot of experience trying to get a clean H. pylori. I don't recall using the Ventana H. pylori but I was using the CellMarque H. pylori for years. It would often have a lot of background as you describe. I run Ventana Benchmarks (XT and Ultra) and a Leica Bond. The slides I would run on the Benchmarks were usually cleaner than those run on the Bond but still not as clean as I would like. I finally switched over to BioCare's H. pylori after many complaints from my pathologists. It has been much cleaner on all of my IHC instruments. Actually, the ones I run on the Ultra are the cleanest I have ever seen. The slides run on the Bond may still have a little background but it is still much cleaner than the ones I used to run with the CellMarque antibody. One of my pathologists who used to complain about the excessive background now calls it his go to stain and is very happy with how crisp it is. The organisms stand out very nicely. BioCare will send you a free sample if you want to try it. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -Original Message- From: Cates, Julia via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, July 30, 2015 8:30 AM To: histonet-boun...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana H.Pylori antibody Good day Histonet, I have seen in the past conversations regarding Ventana's H.Pylori antibody and how dirty looking the stain can be. We have been using this antibody for several years now and have never really been happy with the quality. We have tried the recommended methods to clean up the stain and still we run into repeating the stain and/or complaints from the pathologists. We are considering using a different vendor but my concern is that my efforts will be a lateral move. Is anyone using a product that produces a clean stain or is this something inherent to this antibody? Are the pathologists happy with it and not just tolerating it? Thank you for your suggestions, Julia Cates, HT(ASCP)cm Pathology Coordinator, Pathology Florida Hospital Waterman (352) 253- ext.4346 | Fax: (352) 253-3592 Confidentiality Statement: This email message, including any attachments, is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply to this email and delete the original and all copies of this email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. CONFIDENTIALITY NOTICE: DO NOT FORWARD THIS MESSAGE TO OTHERS WITHOUT PERMISSION OF THE SENDER. This e-mail, including any attachments, may contain confidential or privileged material that is exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, dissemination, copying, or taking any action in reliance on its contents is prohibited. If you have any reason to believe this e-mail was not intended for you, please delete the e-mail and any attachments, and notify the sender immediately. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Re: [Histonet] Ventana H.Pylori antibody
Wow! Thank you all so much for your responses. They have been very helpful; it's so great to have a community of professionals that are so willing to give of their knowledge. Julia Cates, HT(ASCP)cm Pathology Coordinator, Pathology Florida Hospital Waterman (352) 253- ext.4346 | Fax: (352) 253-3592 Confidentiality Statement: This email message, including any attachments, is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply to this email and delete the original and all copies of this email. Message: 1 Date: Thu, 30 Jul 2015 17:06:04 + From: Jeffrey Robinson jrobin...@pathology-associates.com To: Cates, Julia julia.ca...@ahss.org, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana H.Pylori antibody Message-ID: 204A03EB5A7F0A4BB1EEDD52A963829C16D9EB26@PAEXCH1.PathologyAssociates.local Content-Type: text/plain; charset=us-ascii Hi Julia- I have had a lot of experience trying to get a clean H. pylori. I don't recall using the Ventana H. pylori but I was using the CellMarque H. pylori for years. It would often have a lot of background as you describe. I run Ventana Benchmarks (XT and Ultra) and a Leica Bond. The slides I would run on the Benchmarks were usually cleaner than those run on the Bond but still not as clean as I would like. I finally switched over to BioCare's H. pylori after many complaints from my pathologists. It has been much cleaner on all of my IHC instruments. Actually, the ones I run on the Ultra are the cleanest I have ever seen. The slides run on the Bond may still have a little background but it is still much cleaner than the ones I used to run with the CellMarque antibody. One of my pathologists who used to complain about the excessive background now calls it his go to stain and is very happy with how crisp it is. The organisms stand out very nicely. BioCare will send you a free sample if you want to try it. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -Original Message- From: Cates, Julia via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, July 30, 2015 8:30 AM To: histonet-boun...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana H.Pylori antibody Good day Histonet, I have seen in the past conversations regarding Ventana's H.Pylori antibody and how dirty looking the stain can be. We have been using this antibody for several years now and have never really been happy with the quality. We have tried the recommended methods to clean up the stain and still we run into repeating the stain and/or complaints from the pathologists. We are considering using a different vendor but my concern is that my efforts will be a lateral move. Is anyone using a product that produces a clean stain or is this something inherent to this antibody? Are the pathologists happy with it and not just tolerating it? Thank you for your suggestions, Julia Cates, HT(ASCP)cm Pathology Coordinator, Pathology Florida Hospital Waterman (352) 253- ext.4346 | Fax: (352) 253-3592 Confidentiality Statement: This email message, including any attachments, is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply to this email and delete the original and all copies of this email. ___ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] P16
Hello Histonetters, Happy Friday! I need p16 antibody but not sure which vendor provide reliable antibody. I'd appreciate any recommendation. I also need melanoma and prostate ca control blocks, I can exchange with the once I have. Thank you and have a nice weekend. Kadhem Kadhem Alkhenaizi, MS HTL (ASCP) MB, QIHC Lab Manager ExpressMed Labs ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Breast fixation
There are several studies (one listed below) that have addressed the issue of fixation past the recommended 72 hour maximum. In my opinion, the science behind these fixation recommendations is weak at best. In my own experience, I have seen no problem whatsoever going well past 72 hours of fixation. The majority of breast cases will show internal positive controls for ER and PR to validate that the tissue is satisfactory for testing. Remember, working with your pathologist(s) you can perform your own validation on-site for extended fixation time. The real culprit for poor ER, PR, and HER2 IHC results is inadequate fixation. Reference: Tong LC, Nelson N, Tsourigiannis J, et al.: The effect of prolonged fixation on the IHC evaluation of ER, PR, and HER2 expression in invasive breast cancer: A prospective study. Am J Surg Pathol 2011;35:545-552. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -Original Message- From: Heckford, Karen - SMMC-SF via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Wednesday, July 29, 2015 11:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Breast fixation Good Morning, I have a question about breast fixation. I am in a little bit of a pickle with the 6-72 hour rule for the fixation on breast tissue. Friday I am getting 2 breast cases in the afternoon and both will not have the required minimum 6 hour formalin fixation for my per diem to cut early Saturday morning. He will not be able to make it in again until Monday night. The tissue will be about 3-4 hours (this includes time on the processor) over the 72 hour maximum.Does anyone have any suggestions on what can be done? We are a one person show here. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
