[Histonet] Nuclear bubbling

[Vickroy, James via Histonet]

Traditionally we regard nuclear bubbling as incomplete fixation however I'm not 
so sure that nuclear bubbling can't be caused by additional processing 
problems.  This morning I have some GI biopsies that fixed for nearly 18 hrs 
that have a large amount of nuclear bubbling.   We run the biopsies on a "short 
run".   I am wondering if possibly we are not getting rid of all of the water 
and therefore our dehydration steps are not long enough?

Anybody have an idea besides incomplete fixation?

Jim

Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvick...@springfieldclinic.com



This electronic message contains information from Springfield Clinic, LLP that 
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taken on the contents of this information is strictly prohibited. If you have 
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[Histonet] Histology Supervisor Job in North Carolina

[Melissa Owens via Histonet]

Happy Friday Histoland,

I am looking for a qualified Histotech to fill a 2nd Shift Histology Supervisor 
role in North Carolina. Please contact me for more details. Looking for a Tech 
who is experienced in grossing and has some supervisory/lead/senior experience. 
Please reach out to me for more details. Thank you and have a great day!

Melissa Owens
President, Laboratory Staffing
Allied Search Partners

T: 888.388.7571 ext. 102

F: 888.388.7572


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[Histonet] Training ???

[Joseph Maslanka via Histonet]

Can anyone share with me any worksheets you use to evaluate new trainees. 
Embedding, cutting, special staining things like that. How you set target 
goals?
Thanks in advance, Happy Friday.


Joe Maslanka BS, CT,HT (ASCP)
Anatomical Pathology Technical Supervisor
St Peter's Hospital,MT 59601
(P)(406) 447-2406
(F)(406)444-2126 

Give thanks for ALL things.
"Kindness is the language the blind can see & the deaf can hear- Mark 
Twain



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Re: [Histonet] Tissue processing question

[Walter Benton via Histonet]

We use hair wrapping paper used for perms. It is the same paper called "biopsy 
wraps," but at a significant price reduction. You can buy a variety of sizes 
and the wraps do not cause artifacts and are porous enough for ample solution 
penetration. Biopsy paper comes in blue and other colors, but the hair wraps 
only come in white. Our overall experience with them has been great.

Let me know if you need any other information.


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.



-Original Message-
From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, January 29, 2016 12:43 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue processing question

Hello all,

 I was wondering what everyone uses to secure biopsy and scant tissues through 
processing. Also what would you recommend placing breast cores in for 
processing. Having an argument with grossing staff and pathologist about 
whether to use sponges, tissue paper, or something else. Looking for the best 
option that will allow for reagents to penetrate tissue and not leave any 
artifact

--

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs

Doctors Pathology Services, Dover DE
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[Histonet] Tissue processing question

[Charles Riley via Histonet]

Hello all,

 I was wondering what everyone uses to secure biopsy and scant tissues
through processing. Also what would you recommend placing breast cores in
for processing. Having an argument with grossing staff and pathologist
about whether to use sponges, tissue paper, or something else. Looking for
the best option that will allow for reagents to penetrate tissue and not
leave any artifact

-- 

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs

Doctors Pathology Services, Dover DE
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Re: [Histonet] Nuclear bubbling

[Rene J Buesa via Histonet]

"Nuclear bubbling", manifested as round unstained areas in the nucleus, is 
caused by incomplete dehydration of the section before staining. There is a 
review on the subject that I cannot find at this moment.René 

On Friday, January 29, 2016 10:42 AM, "Vickroy, James via Histonet" 
 wrote:
 

 
Traditionally we regard nuclear bubbling as incomplete fixation however I'm not 
so sure that nuclear bubbling can't be caused by additional processing 
problems.  This morning I have some GI biopsies that fixed for nearly 18 hrs 
that have a large amount of nuclear bubbling.  We run the biopsies on a "short 
run".  I am wondering if possibly we are not getting rid of all of the water 
and therefore our dehydration steps are not long enough?

Anybody have an idea besides incomplete fixation?

Jim

Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvick...@springfieldclinic.com



This electronic message contains information from Springfield Clinic, LLP that 
may be confidential, privileged, and/or sensitive. This information is intended 
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intended recipient, be aware that disclosure, copying, distribution, or action 
taken on the contents of this information is strictly prohibited. If you have 
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immediately, by electronic mail, so that arrangements may be made for the 
retrieval of this electronic message. Thank you.
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Re: [Histonet] Sponges for processing biopsies

[Gudrun Lang via Histonet]

Sponges are available in different qualities. We use very soft ones, that
don't hurt the tissue. 
I know, there are also very hard materials on the market, that may render
holes in the underfixed biopsies.

Gudrun

-Ursprüngliche Nachricht-
Von: Lester Raff MD via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Gesendet: Freitag, 29. Jänner 2016 20:05
An: 'histonet@lists.utsouthwestern.edu'
Betreff: [Histonet] Sponges for processing biopsies

WE use the biopsy sponges, THOROUGHLY soaked in formalin. They are easy to
use and not time consuming either for the grosser or the histologist. About
98% of our volume is prostate biopsies, and we do not see the compression
artifact Rene references.





Unrelated blog http://tinyurl.com/down0129


A good weekend to all.

Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203

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Re: [Histonet] Nuclear bubbling artifact

[Gudrun Lang via Histonet]

I've read about a group, that observed living cells during the
fixation-process. They saw bubbling in the first period of contact and
penetration of formaldehyde. After a certain time the bubbles disappeared
again. 
Along this observation for me bubbles are a sign of too short fixation.

Gudrun Lang

-Ursprüngliche Nachricht-
Von: Teri Johnson via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Gesendet: Freitag, 29. Jänner 2016 19:49
An: 'histonet@lists.utsouthwestern.edu'
Betreff: Re: [Histonet] Nuclear bubbling artifact

Hi James,

Nuclear bubbling artifact is most commonly seen in formalin fixed epithelial
cells, and GI biopsies are among those samples that are particularly
susceptible to it. It has been linked to inadequate fixation and also to
heating of slides prior to staining without complete air-drying of the
tissues. I would recommend cutting the block again, air drying the slides
for a time before using heat to melt the wax prior to H stain and see if
the artifact persists.

http://www.cap.org/apps/docs/proficiency_testing/nuclear_bubbling.pdf

Best wishes,
Teri Johnson




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Re: [Histonet] Nuclear bubbling artifact

[Teri Johnson via Histonet]

Hi James,

Nuclear bubbling artifact is most commonly seen in formalin fixed epithelial 
cells, and GI biopsies are among those samples that are particularly 
susceptible to it. It has been linked to inadequate fixation and also to 
heating of slides prior to staining without complete air-drying of the tissues. 
I would recommend cutting the block again, air drying the slides for a time 
before using heat to melt the wax prior to H stain and see if the artifact 
persists.

http://www.cap.org/apps/docs/proficiency_testing/nuclear_bubbling.pdf

Best wishes,
Teri Johnson




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the sole use of the intended recipient(s) and contains information that is 
confidential and proprietary to Genoptix Medical Laboratory or its 
subsidiaries. Any unauthorized review, use, disclosure or distribution is 
prohibited. If you are not the intended recipient, immediately contact the 
sender by e-mail and destroy all copies of the original message.
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Re: [Histonet] Tissue processing question

[Walter Benton via Histonet]

Histoscreen cassettes will work as well. Generally the cassette options are 
expensive and may not work in all cassette printers, if you are using one.

http://www.thermoscientific.com/content/tfs/en/product/tissue-loc-histoscreen-cassettes.html

Ultimately, get samples of whatever you like to use.

From: Caroline Miller [mailto:mi...@3scan.com]
Sent: Friday, January 29, 2016 1:36 PM
To: Walter Benton 
Cc: Charles Riley ; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Tissue processing question

I really like this type:
https://www.fishersci.com/shop/products/starplex-scientific-histoplex-tissue-cassettes-micromesh-chamber-8/p-2782584
(although I buy them from mastertech, but they seem to have dissapeared from 
their website)
They are great for both large tissues, and also biopsies. A long time ago when 
I worked in a clinical lab we used the tissue paper and I found that if 
everything was not heated just right the biopsies would stick and things like 
currettes were hard to scrape up from there, I always thought I was doing the 
tissue damage
yours
mills

On Fri, Jan 29, 2016 at 10:06 AM, Walter Benton via Histonet 
> 
wrote:
We use hair wrapping paper used for perms. It is the same paper called "biopsy 
wraps," but at a significant price reduction. You can buy a variety of sizes 
and the wraps do not cause artifacts and are porous enough for ample solution 
penetration. Biopsy paper comes in blue and other colors, but the hair wraps 
only come in white. Our overall experience with them has been great.

Let me know if you need any other information.


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.



-Original Message-
From: Charles Riley via Histonet 
[mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, January 29, 2016 12:43 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue processing question

Hello all,

 I was wondering what everyone uses to secure biopsy and scant tissues through 
processing. Also what would you recommend placing breast cores in for 
processing. Having an argument with grossing staff and pathologist about 
whether to use sponges, tissue paper, or something else. Looking for the best 
option that will allow for reagents to penetrate tissue and not leave any 
artifact

--

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs

Doctors Pathology Services, Dover DE
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recipient(s) named above and may contain information that is protected from 
disclosure under applicable law. If you are not the intended recipient, or the 
employee or agent responsible for delivering it to the intended recipient, you 
are hereby notified that any dissemination, distribution or copying of this 
transmission is strictly prohibited. If you have received this transmission in 
error, please notify the transmitting person/department immediately by email or 
telephone (410) 581-5881 and delete the message 
without making a copy.

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--
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297
CONFIDENTIALITY NOTICE: The information contained in this electronic message is 
intended solely for the personal and confidential use of the designated 
recipient(s) named above and may contain information that is protected from 
disclosure under applicable law. If you are not the intended recipient, or the 
employee or agent responsible for delivering it to the intended recipient, you 
are hereby notified that any dissemination, distribution or copying of this 
transmission is strictly prohibited. If you have received this transmission in 
error, please notify the transmitting person/department immediately by email or 
telephone (410) 581-5881 and delete the message without making a copy.
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Re: [Histonet] Tissue processing question

[Caroline Miller via Histonet]

I really like this type:
https://www.fishersci.com/shop/products/starplex-scientific-histoplex-tissue-cassettes-micromesh-chamber-8/p-2782584

(although I buy them from mastertech, but they seem to have dissapeared
from their website)

They are great for both large tissues, and also biopsies. A long time ago
when I worked in a clinical lab we used the tissue paper and I found that
if everything was not heated just right the biopsies would stick and things
like currettes were hard to scrape up from there, I always thought I was
doing the tissue damage

yours
mills

On Fri, Jan 29, 2016 at 10:06 AM, Walter Benton via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> We use hair wrapping paper used for perms. It is the same paper called
> "biopsy wraps," but at a significant price reduction. You can buy a variety
> of sizes and the wraps do not cause artifacts and are porous enough for
> ample solution penetration. Biopsy paper comes in blue and other colors,
> but the hair wraps only come in white. Our overall experience with them has
> been great.
>
> Let me know if you need any other information.
>
>
> Walter Benton HT(ASCP)QIHC
> Lab Operations Manager
> Chesapeake Urology Associates
> 806 Landmark Drive, Suite 127
> Glen Burnie, MD 21061
> 443-471-5850 (Direct)
> 410-768-5961 (Lab)
> 410-768-5965 (Fax)
> Chesapeakeurology.com
>
> Voted a Best Place to Work by
> Baltimore and Modern Healthcare
> Magazines.
>
>
>
> -Original Message-
> From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu
> ]
> Sent: Friday, January 29, 2016 12:43 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Tissue processing question
>
> Hello all,
>
>  I was wondering what everyone uses to secure biopsy and scant tissues
> through processing. Also what would you recommend placing breast cores in
> for processing. Having an argument with grossing staff and pathologist
> about whether to use sponges, tissue paper, or something else. Looking for
> the best option that will allow for reagents to penetrate tissue and not
> leave any artifact
>
> --
>
> Charles Riley HT(ASCP)CM
>
> Histopathology Coordinator/ Mohs
>
> Doctors Pathology Services, Dover DE
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> CONFIDENTIALITY NOTICE: The information contained in this electronic
> message is intended solely for the personal and confidential use of the
> designated recipient(s) named above and may contain information that is
> protected from disclosure under applicable law. If you are not the intended
> recipient, or the employee or agent responsible for delivering it to the
> intended recipient, you are hereby notified that any dissemination,
> distribution or copying of this transmission is strictly prohibited. If you
> have received this transmission in error, please notify the transmitting
> person/department immediately by email or telephone (410) 581-5881 and
> delete the message without making a copy.
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297
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Re: [Histonet] Tissue processing question

[Shirley A. Powell via Histonet]

Not advertising but I do a lot of research on tiny pieces of tissue and have 
found the perfect cassette from Cancer Diagnostics.  It is the Vortex 
corner-less ones seen here.
http://cancerdiagnostics.com/index.php/cassettes-accessories/microbiopsy-cassettes-and-specialty-cassettes/vortex-biopsy-cassette.html

They have two sizes in these.  I have to process specimens the size of a gnat's 
eye and they do not get lost.  No corners to deal with.

Shirley





-Original Message-
From: Caroline Miller via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, January 29, 2016 1:36 PM
To: Walter Benton 
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Tissue processing question

I really like this type:
https://www.fishersci.com/shop/products/starplex-scientific-histoplex-tissue-cassettes-micromesh-chamber-8/p-2782584

(although I buy them from mastertech, but they seem to have dissapeared from 
their website)

They are great for both large tissues, and also biopsies. A long time ago when 
I worked in a clinical lab we used the tissue paper and I found that if 
everything was not heated just right the biopsies would stick and things like 
currettes were hard to scrape up from there, I always thought I was doing the 
tissue damage

yours
mills

On Fri, Jan 29, 2016 at 10:06 AM, Walter Benton via Histonet < 
histonet@lists.utsouthwestern.edu> wrote:

> We use hair wrapping paper used for perms. It is the same paper called
> "biopsy wraps," but at a significant price reduction. You can buy a
> variety of sizes and the wraps do not cause artifacts and are porous
> enough for ample solution penetration. Biopsy paper comes in blue and
> other colors, but the hair wraps only come in white. Our overall
> experience with them has been great.
>
> Let me know if you need any other information.
>
>
> Walter Benton HT(ASCP)QIHC
> Lab Operations Manager
> Chesapeake Urology Associates
> 806 Landmark Drive, Suite 127
> Glen Burnie, MD 21061
> 443-471-5850 (Direct)
> 410-768-5961 (Lab)
> 410-768-5965 (Fax)
> Chesapeakeurology.com
>
> Voted a Best Place to Work by
> Baltimore and Modern Healthcare
> Magazines.
>
>
>
> -Original Message-
> From: Charles Riley via Histonet
> [mailto:histonet@lists.utsouthwestern.edu
> ]
> Sent: Friday, January 29, 2016 12:43 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Tissue processing question
>
> Hello all,
>
>  I was wondering what everyone uses to secure biopsy and scant tissues
> through processing. Also what would you recommend placing breast cores
> in for processing. Having an argument with grossing staff and
> pathologist about whether to use sponges, tissue paper, or something
> else. Looking for the best option that will allow for reagents to
> penetrate tissue and not leave any artifact
>
> --
>
> Charles Riley HT(ASCP)CM
>
> Histopathology Coordinator/ Mohs
>
> Doctors Pathology Services, Dover DE
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> CONFIDENTIALITY NOTICE: The information contained in this electronic
> message is intended solely for the personal and confidential use of
> the designated recipient(s) named above and may contain information
> that is protected from disclosure under applicable law. If you are not
> the intended recipient, or the employee or agent responsible for
> delivering it to the intended recipient, you are hereby notified that
> any dissemination, distribution or copying of this transmission is
> strictly prohibited. If you have received this transmission in error,
> please notify the transmitting person/department immediately by email
> or telephone (410) 581-5881 and delete the message without making a copy.
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



--
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297
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[Histonet] Sponges for processing biopsies

[Lester Raff MD via Histonet]

WE use the biopsy sponges, THOROUGHLY soaked in formalin. They are easy to use 
and not time consuming either for the grosser or the histologist. About 98% of 
our volume is prostate biopsies, and we do not see the compression artifact 
Rene references.





Unrelated blog http://tinyurl.com/down0129


A good weekend to all.

Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203

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Re: [Histonet] Tissue processing question

[Michael Ann Jones via Histonet]

LOL, we used cigarette paper worked fine.
Now we use screen cassettes or HistoGel processing if super tiny.


Michael Ann

Providing collaborative diagnostic services,
saving lives today and tomorrow.




On 1/29/16, 11:41 AM, "Walter Benton via Histonet"
 wrote:

>Histoscreen cassettes will work as well. Generally the cassette options
>are expensive and may not work in all cassette printers, if you are using
>one.
>
>http://www.thermoscientific.com/content/tfs/en/product/tissue-loc-histoscr
>een-cassettes.html
>
>Ultimately, get samples of whatever you like to use.
>
>From: Caroline Miller [mailto:mi...@3scan.com]
>Sent: Friday, January 29, 2016 1:36 PM
>To: Walter Benton 
>Cc: Charles Riley ; histonet@lists.utsouthwestern.edu
>Subject: Re: [Histonet] Tissue processing question
>
>I really like this type:
>https://www.fishersci.com/shop/products/starplex-scientific-histoplex-tiss
>ue-cassettes-micromesh-chamber-8/p-2782584
>(although I buy them from mastertech, but they seem to have dissapeared
>from their website)
>They are great for both large tissues, and also biopsies. A long time ago
>when I worked in a clinical lab we used the tissue paper and I found that
>if everything was not heated just right the biopsies would stick and
>things like currettes were hard to scrape up from there, I always thought
>I was doing the tissue damage
>yours
>mills
>
>On Fri, Jan 29, 2016 at 10:06 AM, Walter Benton via Histonet
>u>> wrote:
>We use hair wrapping paper used for perms. It is the same paper called
>"biopsy wraps," but at a significant price reduction. You can buy a
>variety of sizes and the wraps do not cause artifacts and are porous
>enough for ample solution penetration. Biopsy paper comes in blue and
>other colors, but the hair wraps only come in white. Our overall
>experience with them has been great.
>
>Let me know if you need any other information.
>
>
>Walter Benton HT(ASCP)QIHC
>Lab Operations Manager
>Chesapeake Urology Associates
>806 Landmark Drive, Suite 127
>Glen Burnie, MD 21061
>443-471-5850 (Direct)
>410-768-5961 (Lab)
>410-768-5965 (Fax)
>Chesapeakeurology.com
>
>Voted a Best Place to Work by
>Baltimore and Modern Healthcare
>Magazines.
>
>
>
>-Original Message-
>From: Charles Riley via Histonet
>[mailto:histonet@lists.utsouthwestern.edutern.edu>]
>Sent: Friday, January 29, 2016 12:43 PM
>To: 
>histonet@lists.utsouthwestern.edu>
>Subject: [Histonet] Tissue processing question
>
>Hello all,
>
> I was wondering what everyone uses to secure biopsy and scant tissues
>through processing. Also what would you recommend placing breast cores in
>for processing. Having an argument with grossing staff and pathologist
>about whether to use sponges, tissue paper, or something else. Looking
>for the best option that will allow for reagents to penetrate tissue and
>not leave any artifact
>
>--
>
>Charles Riley HT(ASCP)CM
>
>Histopathology Coordinator/ Mohs
>
>Doctors Pathology Services, Dover DE
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Re: [Histonet] Tissue processing question

[Walter Benton via Histonet]

We use hair wrapping paper used for perms. It is the same paper called "biopsy 
wraps," but at a significant price reduction. You can buy a variety of sizes 
and the wraps do not cause artifacts and are porous enough for ample solution 
penetration. Biopsy paper comes in blue and other colors, but the hair wraps 
only come in white. Our overall experience with them has been great.

Let me know if you need any other information.


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.



-Original Message-
From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, January 29, 2016 12:43 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue processing question

Hello all,

 I was wondering what everyone uses to secure biopsy and scant tissues through 
processing. Also what would you recommend placing breast cores in for 
processing. Having an argument with grossing staff and pathologist about 
whether to use sponges, tissue paper, or something else. Looking for the best 
option that will allow for reagents to penetrate tissue and not leave any 
artifact

--

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs

Doctors Pathology Services, Dover DE
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intended solely for the personal and confidential use of the designated 
recipient(s) named above and may contain information that is protected from 
disclosure under applicable law. If you are not the intended recipient, or the 
employee or agent responsible for delivering it to the intended recipient, you 
are hereby notified that any dissemination, distribution or copying of this 
transmission is strictly prohibited. If you have received this transmission in 
error, please notify the transmitting person/department immediately by email or 
telephone (410) 581-5881 and delete the message without making a copy.

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Re: [Histonet] Tissue processing question

[Rene J Buesa via Histonet]

Sponges can cause a compression artifact leaving some sort of "imprint" on the 
surface of the biopsy, especially kidney and prostate Bx.I my experience tissue 
paper is the best option. If you are having difficulties with the wrapping, you 
can use "tea bags".René  

On Friday, January 29, 2016 12:54 PM, Charles Riley via Histonet 
 wrote:
 

 Hello all,

 I was wondering what everyone uses to secure biopsy and scant tissues
through processing. Also what would you recommend placing breast cores in
for processing. Having an argument with grossing staff and pathologist
about whether to use sponges, tissue paper, or something else. Looking for
the best option that will allow for reagents to penetrate tissue and not
leave any artifact

-- 

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs

Doctors Pathology Services, Dover DE
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[Histonet] Clearance Angle on Microtome Blade

[Kelli Goodkowsky via Histonet]

Good afternoon,
Can someone touch base with me regarding the clearance angle of the microtome 
blade?  How would you describe a blade clearance angle that is too great or too 
slight, and what microtomy problems are you likely to see with each?  There 
seems to be conflicting information out there (or at least varying 
perspectives).

Thank you!
Kelli

Kelli Goodkowsky, M.Ed., HT (ASCP)
Director Clinical Education
Histologic Science
Goodwin College
(860) 727-6917
kgoodkow...@goodwin.edu
http://www.goodwin.edu






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