Re: [Histonet] Disposal of Processor

[Morken, Timothy via Histonet]

Fawn, equipment we dispose of (ie, surplus for sale) we just have to clean it. 
For a tissue processor we would run a cleaning cycle, clean up the outside of 
crud and it is good to go. There is no infectious contamination issue. We 
signoff on  a form that it has been decontaminated. It is similar to a form we 
sign if a piece of equipment has to be shipped to a vendor factory for repair 
(like an embedding center or cryostat). 


Tim Morken
Pathology Site Manager, Parnassus 
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center



-Original Message-
From: Fawn Bomar via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, August 10, 2016 10:53 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Disposal of Processor

Does anyone know if you need to decontaminate a tissue processor in any special 
way to dispose of it?  If the tissue has all been fixed before being in the 
processor and there were no CJD cases processed on it, is it considered 
biohazardous?



Thank you

Fawn
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Re: [Histonet] Histonet Digest, Vol 153, Issue 10

[Steve McClain via Histonet]

For once I almost refuse to answer because there is No good answer. This is 
absolutely ludicrous. 
Heck no is my first response but that probably doesn't pass muster.
My state inspectors are probably wanting to have me fill out more firms   forms 
for monthly bleaching. Or adding a bleach step to corrode the delicate inner 
bits.

Perhaps the most cost effective method of disposal i am presently consider it 
may be least expensive to require all lab medical directors to put it in their 
will 
my wish is to be entombed with all 4 of my Kseries VIPs. 
They've been working longer than I have and refuse to die. I promise to retire 
those processors with me buried upright.  Let me stand guard the rest of 
eternity to prevent the currently rampant spread of all diseases spread by 
contaminated.

Shucks. I may have to be buried in Jamaica. I shipped a couple of those 
hazardous contaminated VIPs to Kingston. 

Heck I really do not know why we are pondering a non significant disease. Let 
someone identify a problem first before making a regulation or procedure.

In my search for more data on  the rampant waterski is on fire v71.0xA ICD10 
problem, the processor contamination problem just snuck up on me!!  

I guess we need a decontamination room just to contain the outpouring of 
biohazardous waste every time we rotate solution or annual maintenance. 

Yep, let them cite me if my burial protocol fails the stink test.
Steve A. McClain, MD


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[Histonet] Pathology Partners, LLC HT/HTL Job opening

[Clay Milks via Histonet]

We are growing!  Pathology Partners, LLC in Little Rock, AR is adding an 
additional full time HT/HTL. Great pay and a great work environment. You must 
be a self-motivated, industrious worker. Health, Dental, Vision and 401k 
available.  Please call Clay Milks at 501-687-9220 or email your resume to 
cmi...@psquaredlabs.com


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Re: [Histonet] Freezing biopsies

[Morken, Timothy via Histonet]

Judi, what is the purpose of freezing it? For sectioning, biochemistry, 
histochemistry, or shipping it? It makes a difference. 


Tim Morken
Pathology Site Manager, Parnassus 
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center




-Original Message-
From: Judi Ford via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, August 11, 2016 10:23 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Freezing biopsies

Hi everyone,
Does anyone have experience working with human frozen biopsies? I have a 
question concerning freezing the biopsies. I am just doing general background 
research on this subject at the moment and have not worked with frozen human 
biopsies.

If a biopsy was taken using a 14 gauge needle, in general, what is the length 
and diameter of a biopsy and what would the preferred method of freezing be 
(flash freezing in dry ice or freezing in liquid nitrogen)? What is the method 
for flash freezing in dry ice? What gauge of needles are most commonly used?  
Are most biopsies taken using a 16-18 gauge needle?

Thanks for your help with this. I really appreciate it.
Best regards,
Judi Ford


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[Histonet] Chicken eyes

[Mca Werdler via Histonet]

THank you all for the quick and wonderfull responses! I have some things to
work out now and i will let you all know the result

Thank you all so much

Maarten
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Re: [Histonet] specimen storage cabinets

[Rene J Buesa via Histonet]

Get regular metal cabinets used to store garage items.They are sold at any 
general store (such as HomeDepot or Walmart).René 

On Thursday, August 11, 2016 12:05 PM, Atoska Gentry via Histonet 
 wrote:
 

 Hello, I work for a research facility and we currently have an archive of 
several formalin fixed samples stored in 500ml glass specimen jars; one of our 
PI's recently asked me to conduct a search for storage cabinets specifically 
designed to accommodate these samples. Is anyone aware of the availability of  
such cabinets? The only thing my search has turned up thus far are the 
flammable metal cabinets. But due to limited storage space we have to stack 
these jars and the generic flammable cabinets are not stacked glass specimen 
jar suitable. All ideas & input are welcome. Thank you kindly, Atoska
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[Histonet] Freezing biopsies

[Judi Ford via Histonet]

Hi everyone,
Does anyone have experience working with human frozen biopsies? I have a 
question concerning freezing the biopsies. I am just doing general background 
research on this subject at the moment and have not worked with frozen human 
biopsies.

If a biopsy was taken using a 14 gauge needle, in general, what is the length 
and diameter of a biopsy and what would the preferred method of freezing be 
(flash freezing in dry ice or freezing in liquid nitrogen)? What is the method 
for flash freezing in dry ice? What gauge of needles are most commonly used?  
Are most biopsies taken using a 16-18 gauge needle?

Thanks for your help with this. I really appreciate it.
Best regards,
Judi Ford


STATEMENT OF CONFIDENTIALITY:

The information contained in this electronic message and any attachments to 
this message are intended for the exclusive use of the addressee(s) and may 
contain confidential or privileged information. If you are not an intended 
recipient, or a person responsible for delivering the e-mail to an intended 
recipient, please be advised that you have received this message in error and 
that any use, dissemination, forwarding, printing, or copying is strictly 
prohibited. Please notify the sender at CytomX Therapeutics, Inc., immediately 
and destroy all copies of this message and any attachments.

CytomX Therapeutics, Inc. 
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[Histonet] Chicken eyes

[Mca Werdler via Histonet]

Hello everyone,

I was wondering if you could help me out. I have some chicken eyes for
research and i need a good fixation. My problem is that if i fixated the
eyes in formalin for 48 hours, and i cut open the eyes, the eyes shrink a
lot.

Do i need a different fixative? or do i need longer fixation time? What are
your experiences? I need a fixative, which is good foor IHC.

THank you all

Maarten
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[Histonet] cm1100 leica

[Renee Lightfeldt via Histonet]

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Re: [Histonet] specimen storage cabinets

[Atoska Gentry via Histonet]

Hello, I work for a research facility and we currently have an archive of 
several formalin fixed samples stored in 500ml glass specimen jars; one of our 
PI's recently asked me to conduct a search for storage cabinets specifically 
designed to accommodate these samples. Is anyone aware of the availability of  
such cabinets? The only thing my search has turned up thus far are the 
flammable metal cabinets. But due to limited storage space we have to stack 
these jars and the generic flammable cabinets are not stacked glass specimen 
jar suitable. All ideas & input are welcome. Thank you kindly, Atoska
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