[Histonet] Movat's pentachrome

[Angela Lamberth via Histonet]

Thanks Bob. Somebody else mentioned her name also so I'll be sure to reach
out to her.


THE expert on the Movat pentachrome stain is Akemi Allison, a very senior
histotechnologist who's published about it. I can't remember if she's on
Histonet, but you can contact her on Facebook - I hear from her there quite
often.

I'm not personally familiar with the stain, but I don't think either of
those substitutions will work. You need to find out more about saffron
before you work with it, because it's so expensive.

Bob Richmond
Samurai Pathologist
Maryville TN



-- 
Angela Lamberth
Histology Technician II
Histology Core Lab
La Jolla Institute for Allergy & Immunology
9420 Athena Circle
La Jolla, CA 92037
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[Histonet] Movat's pentachrome

[Angela Lamberth via Histonet]

Hi Terri,

I have used Poly Scientific kits in the past and agree that they are
fabulous. If I were still in anatomic pathology I would wholeheartedly
agree with you. However, I'm now in a research facility and the kit is
simply cost prohibitive. Fortunately, I do now have time for homemade
(a true luxury that I acknowledge is not applicable in AP). Thank you
for your suggestion. :)

PS: This is my first time replying to a post on histonet that did not
come to my email inbox. Did I do it right??


Just wondering why you are compelled to make all the reagents in house
for the Movat's stain.  I have used the kit k042 from Poly Scientic
with fabulous results (and no, I have no gain from recommending them).
Their solutions are wonderfully labeled and can be replenished
individually, or by ordering the kit.  All the time you spend making
and labeling and validating "homemade" would offset any cost increase
from purchasing their premade solutions.  I know.  I did the math.
Just a suggestion.  Sincerely, Terri

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874


-- 
Angela Lamberth
Histology Technician II
Histology Core Lab
La Jolla Institute for Allergy & Immunology
9420 Athena Circle
La Jolla, CA 92037
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Re: [Histonet] Modified Pentachrome

[Angela Lamberth via Histonet]

Thanks for sharing. It's very helpful. Where do you purchase your safran du
gatinais/saffron from? At this point I'm ready to start growing my own
Crocus sativus.

On Thu, Sep 1, 2016 at 12:35 PM, Amos Brooks  wrote:

> Hi,
>  I use Lugol's Iodine all the time for this. It works just fine. I do
> purchase certain chemicals from manufacturers like Lugol's Iodine which I
> get from EMS. Most of the chemicals I make up myself from powder though. I
> have shared the procedure with you via Google Docs. I hope it helps.
>
> Amos
>



-- 
Angela Lamberth
Histology Technician II
Histology Core Lab
La Jolla Institute for Allergy & Immunology
9420 Athena Circle
La Jolla, CA 92037
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[Histonet] Modified Pentachrome

[Amos Brooks via Histonet]

Hi,
 I use Lugol's Iodine all the time for this. It works just fine. I do
purchase certain chemicals from manufacturers like Lugol's Iodine which I
get from EMS. Most of the chemicals I make up myself from powder though. I
have shared the procedure with you via Google Docs. I hope it helps.

Amos
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[Histonet] H. pylori stain

[Amy Johnson via Histonet]

Hello histoland

We currently use Ventana's h. pylori antibody on our Benchmark ultra.  Over the 
last few months our pathologists have been noticing "junk" that is staining 
with this antibody.  It seems to be on the tissue more than in the tissue and 
its not on all slides.  WE have tried changing our waterbath from tap water to 
distilled water with no difference in staining.  We thought maybe our paraffin 
was bad so we stained a "blank" block of paraffin but could not find the 
culprit...Any insight would be appreciated!

Thanks
Amylin Johnson, B.S. HTL(ASCP)
Associates in Pathology
Wausau Wi 54401
715-847-2130

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Re: [Histonet] (no subject)

[Duddey, Aimee via Histonet]

We ran into this situation with TJC.  We had a very limited check off sheet.  
In effort to standardize processes across the laboratory we adopted a program 
very similar to the NY facility whose program is referenced in the TJC leading 
practice library.  It is very rigorous and time consuming but serves its 
purpose well.  Also, CAP has a competency assessment program specifically for 
histology that is a subscription based purchase regardless if you are 
accredited by them.

Aimee

-Original Message-
From: Nirmala Srishan via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, September 01, 2016 1:49 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Hi,

What is everyone doing about the Histology Staff competencies.  I know CLIA has 
several criteria for the med-tech competencies.  Since the Histotechs do not 
report out results, how is everyone implementing this process.  Any information 
is greatly appreciated.


Nirmala 







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patient care, clinical performance and workplace excellence.
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[Histonet] Histotech competency evaluation RE: (no subject)

[Morken, Timothy via Histonet]

Nirmala, Histotechs are not required to have competency assessment under CLIA 
regulations because, as you note, they do not interpret any tests or report any 
results. However, if they do grossing then they do require high complexity 
competency assessment.

Having said that, if there is some concern in an inspection, any deemed 
accreditation agency (CLIA, CAP or JC) inspector, or state inspector,  can ask 
how you determine competency of any lab staff member to do any task. It is up 
to the Laboratory Director to determine how that is done, and how often. An 
institution may do it differently than the standard CLIA regulations outline, 
less often and less rigorous, but will need to convince an inspector that it is 
acceptable. The easiest way is to be sure there will not be any problems is to 
follow the competency assessment criteria for medium and high complexity - that 
is accepted and familiar to the inspectors so they should have no problem 
accepting it for other staff. 

Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
Pathology UC San Francisco Medical Center

-Original Message-
From: Nirmala Srishan via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, September 01, 2016 10:49 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Hi,

What is everyone doing about the Histology Staff competencies.  I know CLIA has 
several criteria for the med-tech competencies.  Since the Histotechs do not 
report out results, how is everyone implementing this process.  Any information 
is greatly appreciated.


Nirmala 







Holy Name Medical Center is ranked among the top hospitals in the nation for 
patient care, clinical performance and workplace excellence.
Click here to learn more.

 Warning: The information contained in this message is privileged and 
CONFIDENTIAL and is intended only for the use of the addressee above. If you 
are not the intended recipient, you are hereby notified that any disclosure, 
copying, distribution, or taking of any action in reliance on the content of 
this message is strictly prohibited. If you have received this communication in 
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Re: [Histonet] Modified Movat's Pentachrome stain

[Bob Richmond via Histonet]

Angela Lamberth at the La Jolla [California] Institute for Allergy &
Immunology asks:

>>I'm gearing up to perform a pentachrome stain. I will be making this in
house and not using a kit. Through searching histonet, I've found a
protocol used by the Children?s Hospital of Philadelphia Pathology Core.
http://pathcore.research.chop.edu/docs/MovatPentachromeStain.pdf
Two things: Iodine crystals are out of the question. Can I replace that
with Lugol's iodine solution or should I just substitute Weigert's for the
hematoxylin in this protocol?
Also, saffron vs tartrazine vs Orange G stain? There is a real difference
in price. Does anybody have a personal preference in terms of quality or
aesthetics? Is paying the extra money for saffron really worth it?<<

THE expert on the Movat pentachrome stain is Akemi Allison, a very senior
histotechnologist who's published about it. I can't remember if she's on
Histonet, but you can contact her on Facebook - I hear from her there quite
often.

I'm not personally familiar with the stain, but I don't think either of
those substitutions will work. You need to find out more about saffron
before you work with it, because it's so expensive.

Bob Richmond
Samurai Pathologist
Maryville TN
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Re: [Histonet] (no subject)

[Morken, Timothy via Histonet]

Nirmala, Histotechs are not required to have competency assessment under CLIA 
regulations because, as you note, they do not interpret any tests or report any 
results. However, if they do grossing then they do require high complexity 
competency assessment.

Having said that, if there is some concern in an inspection, any deemed 
accreditation agency (CLIA, CAP or JC) inspector, or state inspector,  can ask 
how you determine competency of any lab staff member to do any task. It is up 
to the Laboratory Director to determine how that is done, and how often. An 
institution may do it differently than the standard CLIA regulations outline, 
less often and less rigorous, but will need to convince an inspector that it is 
acceptable. The easiest way is to be sure there will not be any problems is to 
follow the competency assessment criteria for medium and high complexity - that 
is accepted and familiar to the inspectors so they should have no problem 
accepting it for other staff. 

Tim Morken
Pathology Site Manager, Parnassus 
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center




-Original Message-
From: Nirmala Srishan via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, September 01, 2016 10:49 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Hi,

What is everyone doing about the Histology Staff competencies.  I know CLIA has 
several criteria for the med-tech competencies.  Since the Histotechs do not 
report out results, how is everyone implementing this process.  Any information 
is greatly appreciated.


Nirmala 







Holy Name Medical Center is ranked among the top hospitals in the nation for 
patient care, clinical performance and workplace excellence.
Click here to learn more.

 Warning: The information contained in this message is privileged and 
CONFIDENTIAL and is intended only for the use of the addressee above. If you 
are not the intended recipient, you are hereby notified that any disclosure, 
copying, distribution, or taking of any action in reliance on the content of 
this message is strictly prohibited. If you have received this communication in 
error, please notify the sender by replying to this message, and then delete it 
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Re: [Histonet] Movat Pentachrome

[Elizabeth Chlipala via Histonet]

If you are not that concerned over the alcian blue portion of the movats you 
can perform an elastic trichrome - that's what we do here.  Mordant like you 
would in Bouins, and rather than stain with weigerts hemastoxylin, stain with 
the elastic portion of the VVG, differentiate like you would for elastic fibers 
and then continue on with the rest of the trichrome, it turns out great.  I 
have an SOP if anyone wants it.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

-Original Message-
From: Forest Blankenship via Histonet 
[mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, September 01, 2016 11:24 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] Movat Pentachrome

Angela, the Movat is a wonderful thing for showing  loss of elastic fibers in 
emphysema and cardiovascular cases. We ran a ton of them in training because 
our lab/histo school was affiliated with the Texas Heart Institute.
Currently at the hospital I work at we use a Kit(Polyscientific)  for the VVG 
part of the stain and Saffron du Galantis for the counterstain. It smells great 
when gently heated.
As far as remarks about a trichrome being able to show the same thing; yeah if 
you also run a VVG on a serial section to show elastic fibers  and you lose the 
awesomeness that is the Movat.

Forest Blankenship, HTL(ASCP)
Histology Manager
Driscoll Children's Hospital
Corpus Christ, TX

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Re: [Histonet] Movat's pentachrome

[Terri Braud via Histonet]

Just wondering why you are compelled to make all the reagents in house for the 
Movat's stain.  I have used the kit k042 from Poly Scientic with fabulous 
results (and no, I have no gain from recommending them).
Their solutions are wonderfully labeled and can be replenished individually, or 
by ordering the kit.  All the time you spend making and labeling and validating 
"homemade" would offset any cost increase from purchasing their premade 
solutions.  I know.  I did the math.
Just a suggestion.  Sincerely, Terri

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874





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[Histonet] (no subject)

[Nirmala Srishan via Histonet]

Hi,

What is everyone doing about the Histology Staff competencies.  I know 
CLIA has several criteria for the med-tech competencies.  Since the 
Histotechs do not report out results, how is everyone implementing this 
process.  Any information is greatly appreciated.


Nirmala 







Holy Name Medical Center is ranked among the top hospitals in the nation 
for patient care, clinical performance and workplace excellence.
Click here to learn more.

 Warning: The information contained in this message is privileged and 
CONFIDENTIAL and is intended only for the use of the addressee above. If 
you are not the intended recipient, you are hereby notified that any 
disclosure, copying, distribution, or taking of any action in reliance on 
the content of this message is strictly prohibited. If you have received 
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[Histonet] Position Available Santa Barbara California Area

[Van Caldwell via Histonet]

29/Aug/16
Requisition: 2067718
Job Title: Technical Support Specialist
Description: Agilent inspires and supports discoveries that advance the
quality of life. We provide life science, diagnostic and applied market
laboratories worldwide with instruments, services, consumables,
applications and expertise. Agilent enables customers to gain the answers
and insights they seek –– so they can do what they do best: improve the
world around us. Information about Agilent is available at www.agilent.com.

Diagnostic Services is looking for an experienced technology leader with a
strong call center support background with medical device hardware and
software to deliver superior support activities for our pathology/histology
instrumentation solutions.

We develop pathology solutions for tissue-based cancer diagnostics. Our
portfolio of solutions includes fully automated systems, all operating in a
highly-regulated market environment. We are using lean lab approaches to
increase our customer's productivity and efficiency while simultaneously
eliminating the chances of operational errors in their workflows.

This is an opportunity to join an enthusiastic and dynamic team and work
closely with application experts, hardware experts, marketing, QA/RA and
directly with customers.

Responsibilities include:
- Primary responsibility is to provide Post Sales Tier 1 support via the
phone to Agilent’s existing customers M-F 6AM to 3PM.
- Provides escalation support to internal resources including Tier II
Technical Support, Field Service, Customer Service and Application
Specialists.
- Monitor the Technical Support Help Desk to respond to inquiries and
requests for support.
- Document all complaints or reportable events per QMS procedures.
- Solves complex problems requiring some technical and business admin
breadth/ depth of knowledge.
Qualifications: - Bachelor’s degree, specialized training/certification, or
equivalent combination of education and experience.
- 5+ year’s relevant experience in similar position.
- Troubleshooting knowledge and skills to complete specialized tasks.
- Competent with Microsoft Office (Word, Excel, PowerPoint, Outlook)
- Some experience with Laboratory Information Systems, Laboratory Equipment
and Laboratory Workflow is a plus.

Agilent Technologies, Inc. is an Equal Employment Opportunity and
Affirmative Action employer. We value diversity at all levels. All
individuals, regardless of personal characteristics, are encouraged to
apply. All qualified applicants will receive consideration for employment
without regard to sex, pregnancy, race, religion or religious creed, color,
gender, gender identity, gender expression, national origin, ancestry,
physical or mental disability, medical condition, genetic information,
marital status, registered domestic partner status, age, sexual
orientation, military or veteran status, protected veteran status, or any
other basis protected by federal, state, local law, ordinance, or
regulation and will not be discriminated against on these bases.

For more information about equal employment opportunity protections, please
view the ‘EEO is the Law’ poster available here:
https://www.dol.gov/ofccp/regs/compliance/posters/pdf/eeopost.pdf,

https://www.dol.gov/ofccp/regs/compliance/posters/pdf/OFCCP_EEO_Supplement_Final_JRF_QA_508c.pdf

Agilent Technologies, Inc., is committed to diversity in the workplace and
strives to support candidates with disabilities. If you have a disability
and need assistance with any part of the application or interview process
or have questions about workplace accessibility, please contact
+1-262-754-5030 (US and Canada only) or email job_post...@agilent.com. EOE
AA M/F/Vet/Disability
Company: Dako
Business: Agilent CrossLab Group
Job Category: Support / Service
Job Sub-Category: Customer Care Tech
Region: Americas
Country or Area: United States
State/Province: California
Town/City: Carpinteria
Shift: Day Job
Job Type: Experienced
Schedule: Full-time
Travel Required: Yes, 10% of the Time
Duration
(Temp Positions Only): Not applicable
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[Histonet] FT OVERNIGHT HT POSTITION Delray Beach, FL

[Delray Beach Pathology Kari Simeone via Histonet]

Hi Histonetters! We are looking for a full time licensed HISTOTECHNOLOGIST here 
in our very busy Delray Florida Dermatology Lab. This is a permanent full time 
NIGHT SHIFT (40 hours) position with benefits (medical/401k/vacation) and shift 
differential. THIS IS A DRUG FREE WORKPLACE. Background check, personality test 
and drug test will be necessary. Sorry, no relocation assistance provided.


***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!!

Please fill out employment application HERE
http://www.indeed.com/m/viewjob?jk=266fccd1fa688bb6=serp
^^you MUST follow this application link to apply! No exceptions.

*full time position Mon-Fri OR Sun-Thurs 10p-6:30AM
*MUST be licensed as a FL HISTOTEHCNOLOGIST ONLY (will be working solo most of 
your shift)
*MUST have at LEAST FIVE (5) years experience (dermatology preferred)
 Please DO NOT respond if no EXPERIENCE!
*VERY proficient in embedding and microtomy
*WILL MOSTLY BE EMBEDDING EXCISION BLOCKS, please know DERMS
*must be self motivated, reliable and a team player
*knowledge in operating Ventana and Leica equipment desired (not necessary)
*some IHC experience preferred but not necessary



Kari M Simeone

Histology/Immunohistochemistry Specialist Supervisor

Alternate Laboratory Supervisor

Delray Beach Technical Laboratory

ADCS Clinics, LLC

Advanced Dermatology and Cosmetic Surgery

Ameriderm

www.advancedderm.com







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Re: [Histonet] Movat Pentachrome

[Forest Blankenship via Histonet]

Angela, the Movat is a wonderful thing for showing  loss of elastic fibers in 
emphysema and cardiovascular cases. We ran a ton of them in training because 
our lab/histo school was affiliated with the Texas Heart Institute.
Currently at the hospital I work at we use a Kit(Polyscientific)  for the VVG 
part of the stain and Saffron du Galantis for the counterstain. It smells great 
when gently heated.
As far as remarks about a trichrome being able to show the same thing; yeah if 
you also run a VVG on a serial section to show elastic fibers  and you lose the 
awesomeness that is the Movat.

Forest Blankenship, HTL(ASCP)
Histology Manager
Driscoll Children's Hospital
Corpus Christ, TX

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[Histonet] FW: NKX 3.1 protocol-

[Baldwin, Kathy via Histonet]

Hello Histonetters
Was wondering if anyone would share their protocol for the above ABY we are 
utilizing the Ventana Ultra and purchased the ABY 3rd party from Cell Marque I 
would appreciate the help the insert doesn't have the standard protocol on it 
like the rest :( just want to be safe.



Thanks
S. Kathy Baldwin
Histology/Cytology Supervisor
Memorial Hospital and Health Care Center
800 West 9th St.
Jasper, Indiana  47546
Office 812-996-0210
Fax 812-996-0232
Cell 812-887-3357





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Re: [Histonet] Modified Movat's Pentachrome stain

[Angela Lamberth via Histonet]

Thank you for the suggestion. I'll ask around when I reconnect with people
at Long Beach this month.

On Thu, Sep 1, 2016 at 7:47 AM, Rene J Buesa  wrote:

> Do you have any contacts at any old histology lab, i.e., one that has been
> in operation for more than 50 years? You may find there iodine crystals and
> ask for a few grams. I used to have a 500 g bottle at my lab (which began
> in 1947).
> René
>
>
> On Thursday, September 1, 2016 10:39 AM, Angela Lamberth <
> alambe...@lji.org> wrote:
>
>
> I would prefer to not substitute anything yet the procedure calls for a
> minimum of 2 grams of iodine which I simply cannot buy in the United States
> thanks to new DEA regulations.
>
> On Thu, Sep 1, 2016 at 7:21 AM, Rene J Buesa  wrote:
>
> Once you start substituting things in an original recipe, the outcome
> cannot be expected to be what the original recipe was supposed to deliver.
> Iodine crystals cannot be substituted by Lugol because, besides the iodine
> also contains its salts. and alcohol. They are two completely different
> things. This is the same as using Harris hematoxylin without mercury, you
> can keep calling it Harris, but it no longer will stain the same. Finally I
> do not see any advantages of Movat's pentachrome over a good Masson. Just
> my opinion but I think you are embarking in a complex process with little
> chance of finishing with the expected results.
> René
>
>
> On Wednesday, August 31, 2016 9:53 PM, Angela Lamberth via Histonet <
> mailto:histonet@lists.utsouthwestern.edu
> > wrote:
>
>
> Hi Histonetters!
>
> I’m gearing up to perform a pentachrome stain. I will be making this in
> house and not using a kit. Through searching histonet, I’ve found a
> protocol used by the Children’s Hospital of Philadelphia Pathology Core.
> http://pathcore.research.chop.edu/docs/MovatPentachromeStain.pdf
>
>
> Two things:  Iodine crystals are out of the question. Can I replace that
> with Lugol’s iodine solution or should I just substitute Weigert’s for the
> hematoxylin in this protocol?
>
>
> Also, saffron vs tartrazine vs Orange G stain.  There is a real difference
> in price. Does anybody have a personal preference in terms of quality or
> aesthetics? Is paying the extra money for saffron really worth it?
>
>
> Thanks!
>
> Angela
>
>
>
> --
> Angela Lamberth
> Histology Technician II
> Histology Core Lab
> La Jolla Institute for Allergy & Immunology
> 9420 Athena Circle
> La Jolla, CA 92037
> __ _
> Histonet mailing list
> mailto:Histonet@lists.utsouthwestern.edu
> 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> --
> Angela Lamberth
> Histology Technician II
> Histology Core Lab
> La Jolla Institute for Allergy & Immunology
> 9420 Athena Circle
> La Jolla, CA 92037
>
>
>


-- 
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Histology Technician II
Histology Core Lab
La Jolla Institute for Allergy & Immunology
9420 Athena Circle
La Jolla, CA 92037
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Re: [Histonet] Modified Movat's Pentachrome stain

[Rene J Buesa via Histonet]

Do you have any contacts at any old histology lab, i.e., one that has been in 
operation for more than 50 years? You may find there iodine crystals and ask 
for a few grams. I used to have a 500 g bottle at my lab (which began in 
1947).René 

On Thursday, September 1, 2016 10:39 AM, Angela Lamberth 
 wrote:
 

 I would prefer to not substitute anything yet the procedure calls for a 
minimum of 2 grams of iodine which I simply cannot buy in the United States 
thanks to new DEA regulations.
On Thu, Sep 1, 2016 at 7:21 AM, Rene J Buesa  wrote:

Once you start substituting things in an original recipe, the outcome cannot be 
expected to be what the original recipe was supposed to deliver.Iodine crystals 
cannot be substituted by Lugol because, besides the iodine also contains its 
salts. and alcohol. They are two completely different things. This is the same 
as using Harris hematoxylin without mercury, you can keep calling it Harris, 
but it no longer will stain the same. Finally I do not see any advantages of 
Movat's pentachrome over a good Masson. Just my opinion but I think you are 
embarking in a complex process with little chance of finishing with the 
expected results.René 

On Wednesday, August 31, 2016 9:53 PM, Angela Lamberth via Histonet 
 wrote:
 

 Hi Histonetters!

I’m gearing up to perform a pentachrome stain. I will be making this in
house and not using a kit. Through searching histonet, I’ve found a
protocol used by the Children’s Hospital of Philadelphia Pathology Core.
http://pathcore.research.chop.edu/docs/MovatPentachromeStain.pdf


Two things:  Iodine crystals are out of the question. Can I replace that
with Lugol’s iodine solution or should I just substitute Weigert’s for the
hematoxylin in this protocol?


Also, saffron vs tartrazine vs Orange G stain.  There is a real difference
in price. Does anybody have a personal preference in terms of quality or
aesthetics? Is paying the extra money for saffron really worth it?


Thanks!

Angela



-- 
Angela Lamberth
Histology Technician II
Histology Core Lab
La Jolla Institute for Allergy & Immunology
9420 Athena Circle
La Jolla, CA 92037
__ _
Histonet mailing list
mailto:Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


   



-- 
Angela LamberthHistology Technician IIHistology Core LabLa Jolla Institute for 
Allergy & Immunology9420 Athena CircleLa Jolla, CA 92037

   
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Modified Movat's Pentachrome stain

[Angela Lamberth via Histonet]

I would prefer to not substitute anything yet the procedure calls for a
minimum of 2 grams of iodine which I simply cannot buy in the United States
thanks to new DEA regulations.

On Thu, Sep 1, 2016 at 7:21 AM, Rene J Buesa  wrote:

> Once you start substituting things in an original recipe, the outcome
> cannot be expected to be what the original recipe was supposed to deliver.
> Iodine crystals cannot be substituted by Lugol because, besides the iodine
> also contains its salts. and alcohol. They are two completely different
> things. This is the same as using Harris hematoxylin without mercury, you
> can keep calling it Harris, but it no longer will stain the same. Finally I
> do not see any advantages of Movat's pentachrome over a good Masson. Just
> my opinion but I think you are embarking in a complex process with little
> chance of finishing with the expected results.
> René
>
>
> On Wednesday, August 31, 2016 9:53 PM, Angela Lamberth via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
>
>
> Hi Histonetters!
>
> I’m gearing up to perform a pentachrome stain. I will be making this in
> house and not using a kit. Through searching histonet, I’ve found a
> protocol used by the Children’s Hospital of Philadelphia Pathology Core.
> http://pathcore.research.chop.edu/docs/MovatPentachromeStain.pdf
>
>
> Two things:  Iodine crystals are out of the question. Can I replace that
> with Lugol’s iodine solution or should I just substitute Weigert’s for the
> hematoxylin in this protocol?
>
>
> Also, saffron vs tartrazine vs Orange G stain.  There is a real difference
> in price. Does anybody have a personal preference in terms of quality or
> aesthetics? Is paying the extra money for saffron really worth it?
>
>
> Thanks!
>
> Angela
>
>
>
> --
> Angela Lamberth
> Histology Technician II
> Histology Core Lab
> La Jolla Institute for Allergy & Immunology
> 9420 Athena Circle
> La Jolla, CA 92037
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>


-- 
Angela Lamberth
Histology Technician II
Histology Core Lab
La Jolla Institute for Allergy & Immunology
9420 Athena Circle
La Jolla, CA 92037
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Modified Movat's Pentachrome stain

[Rene J Buesa via Histonet]

Once you start substituting things in an original recipe, the outcome cannot be 
expected to be what the original recipe was supposed to deliver.Iodine crystals 
cannot be substituted by Lugol because, besides the iodine also contains its 
salts. and alcohol. They are two completely different things. This is the same 
as using Harris hematoxylin without mercury, you can keep calling it Harris, 
but it no longer will stain the same. Finally I do not see any advantages of 
Movat's pentachrome over a good Masson. Just my opinion but I think you are 
embarking in a complex process with little chance of finishing with the 
expected results.René 

On Wednesday, August 31, 2016 9:53 PM, Angela Lamberth via Histonet 
 wrote:
 

 Hi Histonetters!

I’m gearing up to perform a pentachrome stain. I will be making this in
house and not using a kit. Through searching histonet, I’ve found a
protocol used by the Children’s Hospital of Philadelphia Pathology Core.
http://pathcore.research.chop.edu/docs/MovatPentachromeStain.pdf


Two things:  Iodine crystals are out of the question. Can I replace that
with Lugol’s iodine solution or should I just substitute Weigert’s for the
hematoxylin in this protocol?


Also, saffron vs tartrazine vs Orange G stain.  There is a real difference
in price. Does anybody have a personal preference in terms of quality or
aesthetics? Is paying the extra money for saffron really worth it?


Thanks!

Angela



-- 
Angela Lamberth
Histology Technician II
Histology Core Lab
La Jolla Institute for Allergy & Immunology
9420 Athena Circle
La Jolla, CA 92037
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


   
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet