Re: [Histonet] Blood donations for money

[Bob Richmond via Histonet]

Jorge A. Santiago-Blay, PhD asks about blood donation for money. I suppose
he's in the US. I don't think there's any paid donation of whole blood in
the US any more. This is probably a plasmapheresis center, where people
donate twice a week. The red blood cells are returned to the donor. Two
cycles of this are usually done at a session.

Many, though not all, plasma donors are pretty sleazy people. I'd ask the
plasma center first, then complain to local authorities about it. Most of
these plasma centers are franchise operations, and you could complain to
their managers also.

Most plasma products (derivatives) can be sterilized so they don't transmit
viruses. Or so we hope.

Bob Richmond
Samurai Pathologist
Maryville TN
**

> Close to where I leave, there is an establishment for blood "donations".
> Apparently, the establishment pays per donation. I hypothesize that the
> money explains why the place is generally "hopping" (today, ca. 8:30AM,
> there were ca, 25-30 cars parked in front of the establishment; Sunday
> mornings, same story). Regularly, I see trash out of the store (incl. blood
> splatter marks on the sidewalk, gauze, etc.).
>
> Can someone tell me:
> 1. Where can one find information of the internal operations of
> establishments like this?
>
> 2. Where can one report concerns about establishments like this?
>
> 3. More broadly, how can anyone *scientifically* tell whether the blood
> "donated" at those (or any other) establishments is "safe" for use by other
> humans?
>
> Jorge A. Santiago-Blay, PhD
> blaypublishers.com
>
>
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Re: [Histonet] PAX-5

[Tony Henwood (SCHN) via Histonet]

Hi Beth,
We have found the same thing.

You get a moderate staining (half of what you see with most B cell lymphomas) 
with clone 1EW (as supplied with the Leica Bond rtu).
You will get similar results with clone 24 (38%:  Adams, et al (2009). 
Clinical, phenotypic and genetic similarities and disparities between 
post-transplant and classical Hodgkin lymphomas with respect to therapeutic 
targets. Expert opinion on therapeutic targets, 13(10), 1137-1145.)

We have found that using the Rabbit monoclonal antibody to Pax-5 (Clone SP34), 
it seems that, though staining a similar proportion of B cell lymphomas, 
staining is absent from the Reed-Sternberg cells of Hodgin lymphoma.

Our assessment is in its early stages so with further cases, it might be 
clearer.

It seems that not all Pax-5 antibodies react the same

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Principal Scientist, the Children’s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-Original Message-
From: Linda Margraf via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Saturday, 22 July 2017 7:00 AM
To: histonet@lists.utsouthwestern.edu
Cc: 'one...@wvumedicine.org'
Subject: [Histonet] FW: PAX-5

Here is a message I am posting for Beth….


From: "O'neil, Beth" mailto:one...@wvumedicine.org>>
Date: July 21, 2017 at 2:31:24 PM CDT
To: 
"histonet-ow...@lists.utsouthwestern.edu"
 
mailto:histonet-ow...@lists.utsouthwestern.edu>>
Subject: PAX-5
My pathologists are not happy with PAX-5 on the Ventana Ultra; it will 
occasionally show cytoplasmic staining of the Reed-Sternberg cells (supposed to 
be a nuclear stain).  We use Ventana’s clone SP34. I have been trying to 
optimize with the Optiview kit but we are getting so much background staining 
and only sporadic staining of the R-S cells.  I tried a different clone, Cell 
Marque EP156 which was really clean but would not stain the R-S cells at all, 
no matter what we did.   My Ventana technical specialist is stumped as to why 
we’re having so much trouble.  Does anyone else have the same problem or have 
any suggestions?

Beth Ann O’Neil, MT(ASCP)SC, HTL, QIHC
Histology Supervisor, Technical Specialist
Lab:  304 – 293 – 6014
Office:  304 – 293 – 7629
 one...@wvumedicine.org





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