[Histonet] Low Oxygen Sensor/Alarms

[Cooper, Brian via Histonet]

Hi,

Looking at the newly revised LABGEN checklist this afternoon.  GEN.77550 states 
"In areas where liquid nitrogen is used, there are oxygen sensors with a low 
oxygen alarm mounted in an appropriate location and sufficient airflow to 
prevent asphyxiation."   For those of you who already do this, can you please 
tell me the type and model of sensor you're using?

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
bcoo...@chla.usc.edu


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[Histonet] Job Opening in Massachusetts: IHC Lab Supervisor

[Erin Sarricks via Histonet]

HSRL (Histo-Scientific Research Labs) is a leading GLP contract research
organization which specializes in veterinary histopathology since 1999. Our
staff has been committed to scientific integrity and superior
responsiveness to client needs. A career with HSRL offers you potential to
join an established company with exceptional growth opportunity.

HSRL is seeking a qualified Immunohistochemistry Laboratory Supervisor to
join our team at our facility in Worcester, MA.

This position is split between bench histology/IHC and managing a small
team of specialists.

Essential Functions and Responsibilities:

   - Perform routine histology, including tissue processing, fixation,
   embedding, sectioning, staining, and imaging with the highest of quality.
   - Oversee GLP IHC program.
   - Develop Immunohistochemistry stain protocols and adhere to write SOPs.
   - Effectively collaborate with team members and clients
   - Various administrative duties to include recording and maintaining
   detailed records
   - Excellent organizational skills and ability to multi-task
   - Works independently and troubleshoots technical problems

Qualifications:

   - Minimum 3 years of related experience as a histologic technician with
   concentration in veterinary histopathology and IHC techniques.
   - ASCP certified Histotechnician (HT) or Histotechnologist (HTL) or
   eligible, certification preferred.
   - Demonstrated knowledge of and skill in oral and written communication,
   problem solving, adaptability, and teamwork.
   - Excellent organizational skills and ability to multi-task.
   - Experience with Biocare’s IntelliPATH Autostainer is preferred.

A comprehensive benefits package will be offered to the successful
candidate, salary commensurate with experience.

To learn more about HSRL, please visit http://www.hsrl.org

Job Type: Full-time

Experience:

   - employment as a Histo Tech w/concentration in IHC techniques: 3 years
   (Required)
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Re: [Histonet] H for H. pylori?

[Tony Henwood (SCHN) via Histonet]

The following might be useful:

Tazawa, K., & Tsutsumi, Y. (1998). Effect of prolonged staining with 
hematoxylin on detecting Helicobacter pyiori in hematoxylin‐eosin‐stained 
gastric mucosa. Pathology international, 48(6), 448-452.


Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Principal Scientist, the Children’s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 



-Original Message-
From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, 10 October 2018 6:46 AM
To: P Sicurello; HistoNet
Subject: Re: [Histonet] H for H. pylori?

Paula:"Optimizing" H to detect H. piloris is a rout you should not attempt 
because H cannot be "optimized" for that.Giemsa is one way or, even better, 
modified Steiner stain. The only disadvantage modified Steiner has is using 
radioactive uranium nitrate, but I developed a procedure where 0.02% aq. 
phosphotungstic acid solution can be used instead. IHC will be way more 
expensive.
René 

On Tuesday, October 9, 2018 2:03 PM, P Sicurello via Histonet 
 wrote:
 

 Good Morning Listers,

Has anyone out there optimized an H for H. pylori?

Sure we can run a giemsa or and IHC but what fun is that?

Send me your ideas and make the day of our GI pathologists.

Thanks oodles.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

9300 Campus Point Drive

La Jolla, CA 92037
(P): 858-249-5610



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the sender.

Views expressed in this message are those of the individual sender, and are not 
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Re: [Histonet] H for H. pylori?

[Rene J Buesa via Histonet]

Paula:"Optimizing" H to detect H. piloris is a rout you should not attempt 
because H cannot be "optimized" for that.Giemsa is one way or, even better, 
modified Steiner stain. The only disadvantage modified Steiner has is using 
radioactive uranium nitrate, but I developed a procedure where 0.02% aq. 
phosphotungstic acid solution can be used instead. IHC will be way more 
expensive.
René 

On Tuesday, October 9, 2018 2:03 PM, P Sicurello via Histonet 
 wrote:
 

 Good Morning Listers,

Has anyone out there optimized an H for H. pylori?

Sure we can run a giemsa or and IHC but what fun is that?

Send me your ideas and make the day of our GI pathologists.

Thanks oodles.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

9300 Campus Point Drive

La Jolla, CA 92037
(P): 858-249-5610



*Confidentiality Notice*: The information transmitted in this e-mail is
intended only for the person or entity to which it is addressed and may
contain confidential and/or privileged material.  Any review,
retransmission, dissemination or other use of or taking of any action in
reliance upon this information by persons or entities other than the
intended recipient is prohibited.  If you received this e-mail in error,
please contact the sender and delete the material from any computer.
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Re: [Histonet] FW: Leica Bond Max artifact

[Rene J Buesa via Histonet]

Dear Dr. Rosen:
I see nothing wrong in your protocol but your photo 0950 = 09501 seems as if 
there are 2 superimposed sections, one with a lot of brown particles? Is this 
artifact you are referring to? Seems "too organized" to be an artifact but it 
is brown and not red.
These "non DAB" reagents sometimes develop a sort of precipitate even within 
their expiration date. Why have you decided to use this red reagent instead of 
DAB? That could be a better option.
Other than that I am also completely baffled by your problem.
René


 

On Tuesday, October 9, 2018 1:15 PM, Linda Margraf via Histonet 
 wrote:
 

 Here is a message I am posting for Dr. Rosen:
Hello,

I am a longtime admirer of this list, and I am sure someone on here can help us 
out

We have an artifact on our immunohistochemistry (IHC) slides.

I will attempt to attach pictures using the upload tool. In case that doesn’t 
work, I am attaching a google drive link to some jpgs. The artifact is usually 
golden brown to red/pink in color, is clustered, appears both on top of the 
tissue and around it. If we do an aggressive rundown, it appears less red/brown 
and bluer.

We are using a Leica Bond MAX IHC system which is about a year old. We usually 
do two runs a day and 95% of the time we only use a red detection kit. We only 
see this on our red detection kit on our Leica bond. The brown is unaffected. 
The corresponding H slides are fine.
We started having this artifact after a month or two of production. 85% of the 
time we have some sort of artifact. Sometimes its perfect, sometimes it makes 
the tissue unreadable. We haven’t figured out any precipitating factors.

We have tried a number of things, but we still can’t get control of this.
Here is a brief overview of our protocols:
- Processor: Leica ASP300s tissue processor.
-Parrafin = Mercedes HWWX (dual purpose embedding and infiltration parrafin) 
melting point 57c – we tried switching, and it didn’t help.
- We only use distilled water in our baths
- Mercedes Starfrost charged slides
- Oven for 30 min at 62c
- We always use a cold red detection kit
-Sometimes our runs are delayed but not more than 4 hours
- Rundown is done in our stainer (standardized), and slides are coverslipped 
with tape.
- The bulk fluid containers in the bond are cleaned weekly and refrigerated 
between runs.
- The probe is cleaned every 300 slides.
- Mixing wells are cleaned out every two weeks and replaced often.
-Covertiles are well tended to.

We have tried to correct this problem, but we can't seem to fix it.
Any ideas?

https://drive.google.com/open?id=1wUttTweP7SYLRM5pkGOBr0MBzCMrtJSA


Jason R. Rosen, D.O.
Dermatopathologist
Premier Dermatology Partners
4675 Linton Boulevard, Suite 203
Delray Beach, FL 33445
(p) 561-499-5341
(f) 561-499-5343
(e) jro...@totalderms.com


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[Histonet] H for H. pylori?

[P Sicurello via Histonet]

Good Morning Listers,

Has anyone out there optimized an H for H. pylori?

Sure we can run a giemsa or and IHC but what fun is that?

Send me your ideas and make the day of our GI pathologists.

Thanks oodles.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

9300 Campus Point Drive

La Jolla, CA 92037
(P): 858-249-5610



*Confidentiality Notice*: The information transmitted in this e-mail is
intended only for the person or entity to which it is addressed and may
contain confidential and/or privileged material.  Any review,
retransmission, dissemination or other use of or taking of any action in
reliance upon this information by persons or entities other than the
intended recipient is prohibited.  If you received this e-mail in error,
please contact the sender and delete the material from any computer.
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[Histonet] FW: Leica Bond Max artifact

[Linda Margraf via Histonet]

Here is a message I am posting for Dr. Rosen:
Hello,

I am a longtime admirer of this list, and I am sure someone on here can help us 
out

We have an artifact on our immunohistochemistry (IHC) slides.

I will attempt to attach pictures using the upload tool. In case that doesn’t 
work, I am attaching a google drive link to some jpgs. The artifact is usually 
golden brown to red/pink in color, is clustered, appears both on top of the 
tissue and around it. If we do an aggressive rundown, it appears less red/brown 
and bluer.

We are using a Leica Bond MAX IHC system which is about a year old. We usually 
do two runs a day and 95% of the time we only use a red detection kit. We only 
see this on our red detection kit on our Leica bond. The brown is unaffected. 
The corresponding H slides are fine.
We started having this artifact after a month or two of production. 85% of the 
time we have some sort of artifact. Sometimes its perfect, sometimes it makes 
the tissue unreadable. We haven’t figured out any precipitating factors.

We have tried a number of things, but we still can’t get control of this.
Here is a brief overview of our protocols:
- Processor: Leica ASP300s tissue processor.
-Parrafin = Mercedes HWWX (dual purpose embedding and infiltration parrafin) 
melting point 57c – we tried switching, and it didn’t help.
- We only use distilled water in our baths
- Mercedes Starfrost charged slides
- Oven for 30 min at 62c
- We always use a cold red detection kit
-Sometimes our runs are delayed but not more than 4 hours
- Rundown is done in our stainer (standardized), and slides are coverslipped 
with tape.
- The bulk fluid containers in the bond are cleaned weekly and refrigerated 
between runs.
- The probe is cleaned every 300 slides.
- Mixing wells are cleaned out every two weeks and replaced often.
-Covertiles are well tended to.

We have tried to correct this problem, but we can't seem to fix it.
Any ideas?

https://drive.google.com/open?id=1wUttTweP7SYLRM5pkGOBr0MBzCMrtJSA


Jason R. Rosen, D.O.
Dermatopathologist
Premier Dermatology Partners
4675 Linton Boulevard, Suite 203
Delray Beach, FL 33445
(p) 561-499-5341
(f) 561-499-5343
(e) jro...@totalderms.com


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