[Histonet] Sub X in lieu of Clear rite 3
Hi all, We currently use clear rite 3 in our processor and stain-line and have had no problems for many years. It is getting so expensive now so we are considering switching to Sub X. Does anybody have any feedback on this? Thank you in advance! Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] [EXTERNAL] Histonet Digest, Vol 180, Issue 1
Hello Allan, I have been seeing the same thing but with HistoClearII. I have been using alkaline phosphatase substrates on some double chromogenic staining that requires me to either dehydrate and clear with a non-Xylene solvent or just mount with aqueous PVP mounting media. I had thought that it was perhaps some H2O carrying over into my dehydration and clearing but never did resolve fully. Any info that comes of this I would like to be included on as well. Thank you. Kate -Original Message- From: histonet-requ...@lists.utsouthwestern.edu Sent: Thursday, November 1, 2018 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] Histonet Digest, Vol 180, Issue 1 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Instrument to instrument correlation
Hi Paula - I use duplicate microarrays (TMAs), each with at least 10 positive cases and 10 negative cases, one TMA slide on each instrument for each antibody. Passed CAP with no problems. Hope this helps. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal 3. Instrument Correlation (Paula) Date: Thu, 1 Nov 2018 08:01:32 -0700 From: "Paula" Hello everyone, Could you recommend/suggest how the correlation between 2 IHC Bond instruments is performed and written out for them? This has never been brought up before and I'm just starting my research. Thank you in advance, Paula ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] [EXTERNAL] Re: Pinning Gross Specimens
Bryan's advice is spot on. We do the same thing and cut the paraffin into sizes we need. With some specimens, you can pin them down and return them to the containers they arrived in. Provided they arrive in containers appropriate for the size of the specimen...wink, wink!! Douglas A. Porter, HT (ASCP) Pathologist Assistant Anatomic Pathology IT Coordinator Sparrow Center for Laboratory Medicine Department of Pathology 3392 Patient Care Drive Lansing, MI 48911 517-371-9481 (phone) 517-371-9540 (fax) douglas.por...@sparrow.org -Original Message- From: Bryan Llewellyn via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, November 01, 2018 1:41 PM To: White, Lisa M.; histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] Re: [Histonet] Pinning Gross Specimens Warning: This email originated from outside of Sparrow. Do not click links or open attachments unless you recognize the sender and are expecting the message. -- If you can't find suitable pin out boards, they can be made easily enough. Obtain some suitably sized plastic containers with lids and fill them an inch deep with used, filtered, paraffin wax that would otherwise be discarded. After using, just remelt, filter if needed, then harden again. Bryan Llewellyn White, Lisa M. via Histonet wrote: > Hello all, > > Does anyone know of a container with lid that has cork in the bottom? This > would be utilized to pin out gross specimens for fixation. If so can you > send me vendor info? > > Lisa White, HT(ASCP) > James H. Quillen VAMC > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=qgJBLQvENW4Kb9JcrSOXvj11QOUUKGR5N2IUAtns1Jg&r=6pgpgKsLHvt-FitLISss8MQQkPawKdpRw8msCll96Ts&m=8CDk8DLT4_gRkMQLtEWlhjd-HEI1BOr6w2rsJqQ4GiI&s=6eRg8jZIzOA_oYi3cofPPJr2lgpIG3ALmsL8WV8X46k&e= > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=qgJBLQvENW4Kb9JcrSOXvj11QOUUKGR5N2IUAtns1Jg&r=6pgpgKsLHvt-FitLISss8MQQkPawKdpRw8msCll96Ts&m=8CDk8DLT4_gRkMQLtEWlhjd-HEI1BOr6w2rsJqQ4GiI&s=6eRg8jZIzOA_oYi3cofPPJr2lgpIG3ALmsL8WV8X46k&e= CONFIDENTIALITY NOTICE: This email communication may contain private, confidential, or legally privileged information intended for the sole use of the designated and/or duly authorized recipient(s). If you are not the intended recipient or have received this email in error, please notify the sender immediately by email and permanently delete all copies of this email including all attachments without reading them. If you are the intended recipient, secure the contents in a manner that conforms to all applicable state and/or federal requirements related to privacy and confidentiality of such information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Pinning Gross Specimens
If you can't find suitable pin out boards, they can be made easily enough. Obtain some suitably sized plastic containers with lids and fill them an inch deep with used, filtered, paraffin wax that would otherwise be discarded. After using, just remelt, filter if needed, then harden again. Bryan Llewellyn White, Lisa M. via Histonet wrote: Hello all, Does anyone know of a container with lid that has cork in the bottom? This would be utilized to pin out gross specimens for fixation. If so can you send me vendor info? Lisa White, HT(ASCP) James H. Quillen VAMC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Clear-Rite 3 dirty slides
Thanks all, looks like the consensus is that water into the clearing agent causes it and I should change out alcohol more frequently. Allan On Wed, Oct 31, 2018 at 5:46 PM Allan Wang wrote: > Hi histonet, > > For coverslipping Ventana IHC slides I have dish soap, rinse, alcohol 80%, > 100%, 100%, 100%, followed by three Clear-Rite 3 instead of xylene. In the > past I didn't have this issue, but now I frequently get a lot of residue on > the slides after they enter the Clear-Rite. > > If I completely dump all 3 containers of Clear-Rite 3 it gets rid of the > problem but I'd have to do this every month which is wasteful. If I just > dump the first container and move everything up, the problem still remains. > > Images: > https://i.imgur.com/GTQJe2W.jpg > https://i.imgur.com/np8B5P2.jpg > https://i.imgur.com/SLypUAy.jpg > > Anyone know what this contamination is and the best way to reduce it? > > Allan > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Pinning Gross Specimens
Hello all, Does anyone know of a container with lid that has cork in the bottom? This would be utilized to pin out gross specimens for fixation. If so can you send me vendor info? Lisa White, HT(ASCP) James H. Quillen VAMC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Instrument Correlation
Hello everyone, Could you recommend/suggest how the correlation between 2 IHC Bond instruments is performed and written out for them? This has never been brought up before and I'm just starting my research. Thank you in advance, Paula ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
