Re: [Histonet] Poll question

[Cristi Rigazio via Histonet]

Do you mean between specimens or per shift?

Thanks
Cristi

Sent from my iPhone

> On Feb 26, 2020, at 5:24 PM, Charles Riley via Histonet 
>  wrote:
> 
> How often does everyone clean their water-bath?
> 
> -- 
> 
> Charles Riley BS  HT, HTL(ASCP)CM
> 
> Histopathology Coordinator/ Mohs
> ___
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> Histonet@lists.utsouthwestern.edu
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[Histonet] Poll question

[Charles Riley via Histonet]

How often does everyone clean their water-bath?

-- 

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
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[Histonet] 15 years THIS WEEK!! And Our latest Job Opportunities!!

[Pam Barker via Histonet]

Hi Histonetters,
How are you doing today?  
This is an exciting week for me!
This week we are celebrating
RELIA’s 15th year in business.
15 years, 180 months, 782 weeks, 5,475 days, 131,400 hours and loving EVERY
one of the 7,884,000 minute  of it!
RELIA Solutions 2/2005-2/2020 Cheers to 15 more!

Thank You!!

For the past 15 years I have worked exclusively in the nationwide permanent
placement of histology professionals.  

I have felt so privileged to have helped so many people with their careers
and look forward to helping many more!

I strive to bring you the best histology opportunities out there.

SOME of these are RELIA exclusives…
MOST of these offer Sign- on Bonuses and/or Relocation Assistance
ALL of these Companies offer excellent compensation, benefits and great
environments.  
AND THEY ALL ARE READY TO HIRE!!

If You Or Anyone You Know Might Be Interested In Any Of These Positions
Please Contact Me.
You can reach me by email at rel...@earthlink.net   
Toll free at 866-607-3542 or on my cell at 407-353-5070.

Here is my Spotlight Opportunity:
Lead Histology Tech – Run Your Own Lab in Beautiful Cheyenne, WY
I have been engaged exclusively by a growing Private Gastroenterology
Practice in Cheyenne, WY that is in need of a ASCP certified Histotech who
is CLIA qualified to gross.  This is a Full time DAY shift position and my
client offers an excellent pay rate, amazing benefits a beautiful lab to run
on your own and one of the most beautiful places to work in the Country!!
TEXT ME/ EMAIL ME/ CALL ME for more info! 407-353-5070, rel...@earthlink.net


We Have Exciting Opportunities In:
Management:
• California Central CA – IHC Manager
• Wyoming  Cheyenne – Lead Histotech 
• Tennessee   Nashville – 3rd shift Grossing Manager
• Ohio   Columbus – Histology Sales Manager
• Illinois   Chicago – Field Applications Specialist IHC
• Mid East Region Field Applications Specialist IHC
Histotechnician/Histotechnologist
• Florida  Tampa Bay – GI 1st or 2nd Shift
• Florida  Panama City – Dermpath Days!!
• North Carolina   Wilmington – Histotech DAYS!!
• TennesseeKingsport Days Histology Tech 

Text Me/ Call Me 407-353-5070 /Shoot me an email at rel...@earthlink.net for
details on any of these opportunities!
I can also be reached toll free at 866-607-3542.

Thanks-Pam

Right Time, Right Place, Right Move with RELIA!
*15 Years!*
Celebrating 15 years of service exclusively to the Histology Community!

Thank You!
 Pam M. Barker 
Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: rel...@earthlink.net   
https://www.facebook.com/RELIASolutionsforhistologyprofessionals 
www.facebook.com/PamBarkerRELIA 
www.linkedin.com/in/reliasolutions 
www.twitter.com/pamatrelia   
check out our latest opportunities at:
http://www.jobvertise.com/members/relia1 
#jobs4myhistopeeps 
#ilovemyhistopeeps
#histopeeps
Follow my hashtags and make your day great and your career greater!!




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Re: [Histonet] IHC troubleshooting

[Cartun, Richard via Histonet]

Are you using some form of antigen retrieval?  In addition to what has been 
discussed previously, antigen retrieval using heat (HIER) can cause tissue loss 
especially collagen.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
richard.car...@hhchealth.org

-Original Message-
From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, February 26, 2020 9:44 AM
To: Histo List
Subject: [Histonet] IHC troubleshooting

EXTERNAL email from Outside HHC! Do NOT open attachments or click links from 
unknown senders.

Our pathologists are complaining that chunks of the dermis are missing from IHC 
slides yet the entire section is present prior to staining.

Does anyone have any ideas what could cause the tissue to not adhere to the
slides throughout the staining process? We use the Leica Bond stainers.

--

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
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[Histonet] 15 years THIS WEEK!! And Our latest Job Opportunities!!

[Pam Barker via Histonet]

Hi Histonetters,
How are you doing today?  
This is an exciting week for me!
This week we are celebrating
RELIA’s 15th year in business.
15 years, 180 months, 782 weeks, 5,475 days, 131,400 hours and loving EVERY
one of the 7,884,000 minute  of it!
RELIA Solutions 2/2005-2/2020 Cheers to 15 more!

Thank You!!

For the past 15 years I have worked exclusively in the nationwide permanent
placement of histology professionals.  

I have felt so privileged to have helped so many people with their careers
and look forward to helping many more!

I strive to bring you the best histology opportunities out there.

SOME of these are RELIA exclusives…
MOST of these offer Sign- on Bonuses and/or Relocation Assistance
ALL of these Companies offer excellent compensation, benefits and great
environments.  
AND THEY ALL ARE READY TO HIRE!!

If You Or Anyone You Know Might Be Interested In Any Of These Positions
Please Contact Me.
You can reach me by email at rel...@earthlink.net   
Toll free at 866-607-3542 or on my cell at 407-353-5070.

Here is my Spotlight Opportunity:
Lead Histology Tech – Run Your Own Lab in Beautiful Cheyenne, WY
I have been engaged exclusively by a growing Private Gastroenterology
Practice in Cheyenne, WY that is in need of a ASCP certified Histotech who
is CLIA qualified to gross.  This is a Full time DAY shift position and my
client offers an excellent pay rate, amazing benefits a beautiful lab to run
on your own and one of the most beautiful places to work in the Country!!
TEXT ME/ EMAIL ME/ CALL ME for more info! 407-353-5070, rel...@earthlink.net


We Have Exciting Opportunities In:
Management:
• California Central CA – IHC Manager
• Wyoming  Cheyenne – Lead Histotech 
• Tennessee   Nashville – 3rd shift Grossing Manager
• Ohio   Columbus – Histology Sales Manager
• Illinois   Chicago – Field Applications Specialist IHC
• Mid East Region Field Applications Specialist IHC
Histotechnician/Histotechnologist
• Florida  Tampa Bay – GI 1st or 2nd Shift
• Florida  Panama City – Dermpath Days!!
• North Carolina   Wilmington – Histotech DAYS!!
• TennesseeKingsport Days Histology Tech 

Text Me/ Call Me 407-353-5070 /Shoot me an email at rel...@earthlink.net for
details on any of these opportunities!
I can also be reached toll free at 866-607-3542.

Thanks-Pam

Right Time, Right Place, Right Move with RELIA!
*15 Years!*
Celebrating 15 years of service exclusively to the Histology Community!

Thank You!
 Pam M. Barker 
Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: rel...@earthlink.net   
https://www.facebook.com/RELIASolutionsforhistologyprofessionals 
www.facebook.com/PamBarkerRELIA 
www.linkedin.com/in/reliasolutions 
www.twitter.com/pamatrelia   
check out our latest opportunities at:
http://www.jobvertise.com/members/relia1 
#jobs4myhistopeeps 
#ilovemyhistopeeps
#histopeeps
Follow my hashtags and make your day great and your career greater!!


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Re: [Histonet] IHC Troubleshooting

[Greg Dobbin via Histonet]

Hi Charles,
It adhesion problems can arise from several sources, some have already been
mentioned. Here are two that I have experienced:
1) Bad slides. Either a manufacturing defect such that the positive charge
is insufficient or the slides were somehow compromised after opening them
on the bench (perhaps exposed to moisture thus removing the charge).
Sometimes a new lot number be better but sometimes switching brands is the
quickest fix.
2) Ensuring all water is removed from under the section. I find that with
some charged slides (many in fact), water is trapped under the section and
does not drain out on standing and does not drain or evaporate when baking
at 60C. If the slide with trapped water is put on the instrument with
trapped water, the dewax step will lift much of the tissue.
Cheers,
-- 
*Greg Dobbin*
1205 Pleasant Grove Rd
RR#2 York,
PE  C0A 1P0


*Everything in moderation...even moderation itself**!*
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Re: [Histonet] IHC troubleshooting

[Abdulbasit Andijany via Histonet]

In my experience;
If the the specimen is under-fixed or under-processed, the section will be 
sloughed off during IHC staining.

I find proper fixation time and drying the slides at room temperature (before 
baking them in oven) optimal.

Andy



Abdulbasit Andijany (Andy)
AMLS, BBMS, MSc, CT (ASCP)CM, HTL (ASCPI)
King Fahad Armed Forces Hospital, Jeddah, Saudi Arabia


On 26 Feb 2020, at 18:35, John Garratt via Histonet 
mailto:histonet@lists.utsouthwestern.edu>> 
wrote:

Do you float and pick up your sections from a water bath of distilled water 
with no adhesive added? If not, doing that will help.

John

On Wed, Feb 26, 2020 at 6:41 AM, Charles Riley via Histonet 
mailto:histonet@lists.utsouthwestern.edu>> 
wrote:

Our pathologists are complaining that chunks of the dermis are missing from
IHC slides yet the entire section is present prior to staining.

Does anyone have any ideas what could cause the tissue to not adhere to the
slides throughout the staining process? We use the Leica Bond stainers.

--

Charles Riley BS HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
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Re: [Histonet] IHC troubleshooting

[John Garratt via Histonet]

Do you float and pick up your sections from a water bath of distilled water 
with no adhesive added? If not, doing that will help.

John

On Wed, Feb 26, 2020 at 6:41 AM, Charles Riley via Histonet 
 wrote:

> Our pathologists are complaining that chunks of the dermis are missing from
> IHC slides yet the entire section is present prior to staining.
>
> Does anyone have any ideas what could cause the tissue to not adhere to the
> slides throughout the staining process? We use the Leica Bond stainers.
>
> --
>
> Charles Riley BS HT, HTL(ASCP)CM
>
> Histopathology Coordinator/ Mohs
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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[Histonet] IHC troubleshooting

[Charles Riley via Histonet]

Our pathologists are complaining that chunks of the dermis are missing from
IHC slides yet the entire section is present prior to staining.

Does anyone have any ideas what could cause the tissue to not adhere to the
slides throughout the staining process? We use the Leica Bond stainers.

-- 

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
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[Histonet] differences in job descriptions

[Eck, Allison via Histonet]

I know this topic has probably been addressed many times before and I do not 
wish to start the certified vs non certified debate again.  However, I must ask 
for those of you who have both working in your lab, do their job descriptions 
and responsibilities differ between those who are certified and those who are 
not?  If they do differ, what are the differences?

Thanks
Allison


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