Re: [Histonet] FW: Microtome at home
"Taking control of the situation is key." It's very interesting to me that most of the responses have to do with institutionalized bureaucratic ideas (safety, insurance, liability, regulations) rather than how to get things done. I was thinking about how Virchow and Henle and other pioneers would have been able to get anything done in the face of such reverence for the stultifying bureaucracy that is the enemy of effectiveness. Afraid to decide, no action is taken. /"Auftragstaktik/ can be seen as a doctrine within which formal rules can be selectively suspended in order to overcome "friction"." E. Wayne Johnson DVM Enable AgTech Beijing Mark Tarango via Histonet wrote: I had heard that CLIA was relaxing things and is not requiring a new # to work from home right now. Best to check on the regulatory but FFPE isn't typically infectious. The ideal spot would in the garage and not the kitchen though. On Thu, Apr 16, 2020 at 3:47 PM Roxana Robinson via Histonet < histonet@lists.utsouthwestern.edu> wrote: I do not agree with this in our current situation or actually any situation. There are quidelines in place with CLIA, OHSA and CAP for protecting not only the patient but also the employee. Whether research or not. Roxana Robinson On Apr 16, 2020, at 4:58 PM, Patsy Ruegg via Histonet < histonet@lists.utsouthwestern.edu> wrote: I agree with this point and as far as clocking in and out, I would think you could work out something like getting paid piece mill, perhaps charge per slide or block cut, that way you could do it on your own time and not have to clock in. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: Joseph Saby Sent: Thursday, April 16, 2020 8:03 AM To: Porter, Amy ; Porter, Amy via Histonet < histonet@lists.utsouthwestern.edu>; histonet@lists.utsouthwestern.edu < histonet@lists.utsouthwestern.edu>; Steven Crochiere Subject: Re: [Histonet] FW: Microtome at home You will need to make sure all pertinent SOPs and EOPs are followed, as well as all safety guidelines/protocols. Just because it is not human tissue doesn't mean that it can't have its share of nasties. Joe Saby Sent from Yahoo Mail on Android On Thu, Apr 16, 2020 at 8:21 AM, Porter, Amy via Histonet< histonet@lists.utsouthwestern.edu> wrote: Make sure of insurance coverage and safety for the employee and that they are covered in case of injury - are they still clocking in and out in some fashion. just thinking in a bigger box. From: Steven Crochiere via Histonet Sent: Thursday, April 16, 2020 6:36 AM To: histonet@lists.utsouthwestern.edu CLIA would need to inspect the set up in the person home. The same goes for our pathologists who read slide at home. Steve -Original Message- From: raestask via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Wednesday, April 15, 2020 7:51 PM To: Jamie Watson ; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Microtome at home I wouldn't think there would be any problem.Rae Staskiewicz HT(ASCP)Sent from my Verizon, Samsung Galaxy smartphone Original message From: Jamie Watson via Histonet < histonet@lists.utsouthwestern.edu> Date: 4/15/20 6:44 PM (GMT-06:00) To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome at home Hello all,Our pathologist has come up with the idea of sending a microtome and waterbath home to someone that cannot come to work due to COVID 19. We are a research lab and work with mouse and rat tissue. Does anyone know of any issues with doing this? I have never heard of anyone cutting slides at home other than someone with a private business.Thank you.Jamie___Histonet mailing listHistonet@lists.utsouthwestern.eduhttp:// lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!nwH0INyWP6lMeJ_Devv2eaelHE25_36kcLQnnnBFaO46Y8_BkxEnT0U_DaplXA$ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!nwH0INyWP6lMeJ_Devv2eaelHE25_36kcLQnnnBFaO46Y8_BkxEnT0U_DaplXA$ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu
Re: [Histonet] FW: Microtome at home
I had heard that CLIA was relaxing things and is not requiring a new # to work from home right now. Best to check on the regulatory but FFPE isn't typically infectious. The ideal spot would in the garage and not the kitchen though. On Thu, Apr 16, 2020 at 3:47 PM Roxana Robinson via Histonet < histonet@lists.utsouthwestern.edu> wrote: > I do not agree with this in our current situation or actually any > situation. > There are quidelines in place with CLIA, OHSA and CAP for protecting not > only the patient but also the employee. Whether research or not. > > > Roxana Robinson > > > On Apr 16, 2020, at 4:58 PM, Patsy Ruegg via Histonet < > histonet@lists.utsouthwestern.edu> wrote: > > > > I agree with this point and as far as clocking in and out, I would > think you could work out something like getting paid piece mill, perhaps > charge per slide or block cut, that way you could do it on your own time > and not have to clock in. > > > > > > Patsy Ruegg, HT(ASCP)QIHC > > Ruegg IHC Consulting > > 40864 E Arkansas Ave > > Bennett, CO 80102 > > H 303-644-4538 > > C 720-281-5406 > > prueg...@hotmail.com > > > > > > > > From: Joseph Saby > > Sent: Thursday, April 16, 2020 8:03 AM > > To: Porter, Amy ; Porter, Amy via Histonet < > histonet@lists.utsouthwestern.edu>; histonet@lists.utsouthwestern.edu < > histonet@lists.utsouthwestern.edu>; Steven Crochiere > > > Subject: Re: [Histonet] FW: Microtome at home > > > > > > You will need to make sure all pertinent SOPs and EOPs are followed, as > well as all safety guidelines/protocols. Just because it is not human > tissue doesn't mean that it can't have its share of nasties. > > Joe Saby > > > > Sent from Yahoo Mail on Android > > > > On Thu, Apr 16, 2020 at 8:21 AM, Porter, Amy via Histonet< > histonet@lists.utsouthwestern.edu> wrote: Make sure of insurance > coverage and safety for the employee and that they are covered in case of > injury - are they still clocking in and out in some fashion. just > thinking in a bigger box. > > > > > > From: Steven Crochiere via Histonet > > Sent: Thursday, April 16, 2020 6:36 AM > > To: histonet@lists.utsouthwestern.edu > > > Subject: [Histonet] FW: Microtome at home > > > > Jaime, > > > > I don't see a problem with a research setting. If it was patient care, > CLIA would need to inspect the set up in the person home. The same goes for > our pathologists who read slide at home. > > > > Steve > > > > -Original Message- > > From: raestask via Histonet [mailto:histonet@lists.utsouthwestern.edu] > > Sent: Wednesday, April 15, 2020 7:51 PM > > To: Jamie Watson ; > Histonet@lists.utsouthwestern.edu > > Subject: Re: [Histonet] Microtome at home > > > > I wouldn't think there would be any problem.Rae Staskiewicz HT(ASCP)Sent > from my Verizon, Samsung Galaxy smartphone > > Original message From: Jamie Watson via Histonet < > histonet@lists.utsouthwestern.edu> Date: 4/15/20 6:44 PM (GMT-06:00) > To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome at > home Hello all,Our pathologist has come up with the idea of sending a > microtome and waterbath home to someone that cannot come to work due to > COVID 19. We are a research lab and work with mouse and rat tissue. Does > anyone know of any issues with doing this? I have never heard of anyone > cutting slides at home other than someone with a private business.Thank > you.Jamie___Histonet mailing > listHistonet@lists.utsouthwestern.eduhttp:// > lists.utsouthwestern.edu/mailman/listinfo/histonet > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!nwH0INyWP6lMeJ_Devv2eaelHE25_36kcLQnnnBFaO46Y8_BkxEnT0U_DaplXA$ > > > > > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!nwH0INyWP6lMeJ_Devv2eaelHE25_36kcLQnnnBFaO46Y8_BkxEnT0U_DaplXA$ > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Recommended thickness of Amyloid sections
Agree. 8-10 microns cut just prior to staining. Roxana Robinson > On Apr 16, 2020, at 3:50 PM, raestask via Histonet > wrote: > > Should also be cut on the day they are to be stained.Rae Staskiewicz > HT(ASCP)Sent from my Verizon, Samsung Galaxy smartphone > Original message From: Paula Keene Pierce via Histonet > Date: 4/16/20 3:24 PM (GMT-06:00) To: > histonet@lists.utsouthwestern.edu, Ken M Subject: Re: > [Histonet] Recommended thickness of Amyloid sections As this is my 41 year of > being a registered HT, I was taught that slides for amyloid are to be cut at > 8-10µm.Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, > Inc.5830 N Blue Lake DriveNorman, OK 73069PH > 405-759-3953http://www.excaliburpathology.comA sharp knife is nothing without > a sharp eye. - Klingon Proverb On Thursday, April 16, 2020, 03:18:44 PM > CDT, Ken M via Histonet wrote:Hello > All. We have always cut all of our histology control slides at 5m. We were > told today that it is common practice to cut Amyloid at 8m? Is this your > experience?Ken___Histonet mailing > listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___Histonet mailing > listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] FW: Microtome at home
I do not agree with this in our current situation or actually any situation. There are quidelines in place with CLIA, OHSA and CAP for protecting not only the patient but also the employee. Whether research or not. Roxana Robinson > On Apr 16, 2020, at 4:58 PM, Patsy Ruegg via Histonet > wrote: > > I agree with this point and as far as clocking in and out, I would think you > could work out something like getting paid piece mill, perhaps charge per > slide or block cut, that way you could do it on your own time and not have to > clock in. > > > Patsy Ruegg, HT(ASCP)QIHC > Ruegg IHC Consulting > 40864 E Arkansas Ave > Bennett, CO 80102 > H 303-644-4538 > C 720-281-5406 > prueg...@hotmail.com > > > > From: Joseph Saby > Sent: Thursday, April 16, 2020 8:03 AM > To: Porter, Amy ; Porter, Amy via Histonet > ; histonet@lists.utsouthwestern.edu > ; Steven Crochiere > Subject: Re: [Histonet] FW: Microtome at home > > > You will need to make sure all pertinent SOPs and EOPs are followed, as well > as all safety guidelines/protocols. Just because it is not human tissue > doesn't mean that it can't have its share of nasties. > Joe Saby > > Sent from Yahoo Mail on Android > > On Thu, Apr 16, 2020 at 8:21 AM, Porter, Amy via > Histonet wrote: Make sure of insurance > coverage and safety for the employee and that they are covered in case of > injury - are they still clocking in and out in some fashion. just > thinking in a bigger box. > > > From: Steven Crochiere via Histonet > Sent: Thursday, April 16, 2020 6:36 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] FW: Microtome at home > > Jaime, > > I don't see a problem with a research setting. If it was patient care, CLIA > would need to inspect the set up in the person home. The same goes for our > pathologists who read slide at home. > > Steve > > -Original Message- > From: raestask via Histonet [mailto:histonet@lists.utsouthwestern.edu] > Sent: Wednesday, April 15, 2020 7:51 PM > To: Jamie Watson ; Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Microtome at home > > I wouldn't think there would be any problem.Rae Staskiewicz HT(ASCP)Sent from > my Verizon, Samsung Galaxy smartphone > Original message From: Jamie Watson via Histonet > Date: 4/15/20 6:44 PM (GMT-06:00) To: > Histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome at home Hello > all,Our pathologist has come up with the idea of sending a microtome and > waterbath home to someone that cannot come to work due to COVID 19. We are a > research lab and work with mouse and rat tissue. Does anyone know of any > issues with doing this? I have never heard of anyone cutting slides at home > other than someone with a private business.Thank > you.Jamie___Histonet mailing > listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!nwH0INyWP6lMeJ_Devv2eaelHE25_36kcLQnnnBFaO46Y8_BkxEnT0U_DaplXA$ > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!nwH0INyWP6lMeJ_Devv2eaelHE25_36kcLQnnnBFaO46Y8_BkxEnT0U_DaplXA$ > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microtome at home
I have done it, but you are right, I had my own private business, not sure why it would be a problem, especially for research. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: Jamie Watson Sent: Wednesday, April 15, 2020 5:44 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome at home Hello all, Our pathologist has come up with the idea of sending a microtome and waterbath home to someone that cannot come to work due to COVID 19. We are a research lab and work with mouse and rat tissue. Does anyone know of any issues with doing this? I have never heard of anyone cutting slides at home other than someone with a private business. Thank you. Jamie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] FW: Microtome at home
I agree with this point and as far as clocking in and out, I would think you could work out something like getting paid piece mill, perhaps charge per slide or block cut, that way you could do it on your own time and not have to clock in. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: Joseph Saby Sent: Thursday, April 16, 2020 8:03 AM To: Porter, Amy ; Porter, Amy via Histonet ; histonet@lists.utsouthwestern.edu ; Steven Crochiere Subject: Re: [Histonet] FW: Microtome at home You will need to make sure all pertinent SOPs and EOPs are followed, as well as all safety guidelines/protocols. Just because it is not human tissue doesn't mean that it can't have its share of nasties. Joe Saby Sent from Yahoo Mail on Android On Thu, Apr 16, 2020 at 8:21 AM, Porter, Amy via Histonet wrote: Make sure of insurance coverage and safety for the employee and that they are covered in case of injury - are they still clocking in and out in some fashion. just thinking in a bigger box. From: Steven Crochiere via Histonet Sent: Thursday, April 16, 2020 6:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Microtome at home Jaime, I don't see a problem with a research setting. If it was patient care, CLIA would need to inspect the set up in the person home. The same goes for our pathologists who read slide at home. Steve -Original Message- From: raestask via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Wednesday, April 15, 2020 7:51 PM To: Jamie Watson ; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Microtome at home I wouldn't think there would be any problem.Rae Staskiewicz HT(ASCP)Sent from my Verizon, Samsung Galaxy smartphone Original message From: Jamie Watson via Histonet Date: 4/15/20 6:44 PM (GMT-06:00) To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome at home Hello all,Our pathologist has come up with the idea of sending a microtome and waterbath home to someone that cannot come to work due to COVID 19. We are a research lab and work with mouse and rat tissue. Does anyone know of any issues with doing this? I have never heard of anyone cutting slides at home other than someone with a private business.Thank you.Jamie___Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!nwH0INyWP6lMeJ_Devv2eaelHE25_36kcLQnnnBFaO46Y8_BkxEnT0U_DaplXA$ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!nwH0INyWP6lMeJ_Devv2eaelHE25_36kcLQnnnBFaO46Y8_BkxEnT0U_DaplXA$ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Recommended thickness of Amyloid sections
I agree, Slightly thicker makes the polarisation easier to see (personal experience). I would love to see a study comparing section thickness Vs polarisation characteristics. Anyone interested? (being a kids hospital we rarely see amyloidosis) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children’s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA From: Paula Keene Pierce via Histonet Sent: Friday, 17 April 2020 06:24 To: histonet@lists.utsouthwestern.edu; Ken M Subject: Re: [Histonet] Recommended thickness of Amyloid sections As this is my 41 year of being a registered HT, I was taught that slides for amyloid are to be cut at 8-10µm. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Thursday, April 16, 2020, 03:18:44 PM CDT, Ken M via Histonet wrote: Hello All. We have always cut all of our histology control slides at 5m. We were told today that it is common practice to cut Amyloid at 8m? Is this your experience? Ken ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Recommended thickness of Amyloid sections
Ken, yes, 8 to 10 um. The extra thickness make the bi-refringence under polarized light brighter. Also the deposits can be variable so even with the light microscope the reddish deposits will stain stronger with thicker sections. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: Ken M via Histonet Sent: Thursday, April 16, 2020 1:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recommended thickness of Amyloid sections Hello All. We have always cut all of our histology control slides at 5m. We were told today that it is common practice to cut Amyloid at 8m? Is this your experience? Ken ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet=DwICAg=iORugZls2LlYyCAZRB3XLg=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA=zu56n7jP8NfTVcr03S3ER1yqzKYomhe2VIC5gz5CiHI=OTIHpP1NshAzBBAOzuP2Ljiz166ODsMhL_CDtbvQJgs= ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Recommended thickness of Amyloid sections
I work at a Dermatology lab and our protocol for amyloid is 8-10 microns. Sent from my iPhone > On Apr 16, 2020, at 4:19 PM, Ken M via Histonet > wrote: > > Hello All. We have always cut all of our histology control slides at 5m. > We were told today that it is common practice to cut Amyloid at 8m? Is this > your experience? > > Ken > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Recommended thickness of Amyloid sections
Should also be cut on the day they are to be stained.Rae Staskiewicz HT(ASCP)Sent from my Verizon, Samsung Galaxy smartphone Original message From: Paula Keene Pierce via Histonet Date: 4/16/20 3:24 PM (GMT-06:00) To: histonet@lists.utsouthwestern.edu, Ken M Subject: Re: [Histonet] Recommended thickness of Amyloid sections As this is my 41 year of being a registered HT, I was taught that slides for amyloid are to be cut at 8-10µm.Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.comA sharp knife is nothing without a sharp eye. - Klingon Proverb On Thursday, April 16, 2020, 03:18:44 PM CDT, Ken M via Histonet wrote: Hello All. We have always cut all of our histology control slides at 5m. We were told today that it is common practice to cut Amyloid at 8m? Is this your experience?Ken___Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ___Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Recommended thickness of Amyloid sections
As this is my 41 year of being a registered HT, I was taught that slides for amyloid are to be cut at 8-10µm. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Thursday, April 16, 2020, 03:18:44 PM CDT, Ken M via Histonet wrote: Hello All. We have always cut all of our histology control slides at 5m. We were told today that it is common practice to cut Amyloid at 8m? Is this your experience? Ken ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Recommended thickness of Amyloid sections
Hello All. We have always cut all of our histology control slides at 5m. We were told today that it is common practice to cut Amyloid at 8m? Is this your experience? Ken ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CAP proficiency testing
The Canadian Pathology Quality Assurance programme runs IHC and FISH EQA internationally. We have performed PD-L1 EQA previously and we will be running a challenge January 2021. Previous EQA reports are available on line. Our website is www.cpqa.ca. We work with regularity authorities to provide compliance information when requested by the participant. John On Thu, Apr 16, 2020 at 10:38 AM, Charles Riley via Histonet wrote: > Does anyone use another vendor besides CAP to perform their PT > requirements? > > If so, who do you use and how do you record it for inspection purposes? > > I am looking for a PD-L1 PT specifically but will take any other options as > well as we are trying to help our budget. > > -- > > Charles Riley BS HT, HTL(ASCP)CM > > Histopathology Coordinator/ Mohs > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CAP proficiency testing
Does anyone use another vendor besides CAP to perform their PT requirements? If so, who do you use and how do you record it for inspection purposes? I am looking for a PD-L1 PT specifically but will take any other options as well as we are trying to help our budget. -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] FW: Microtome at home
You will need to make sure all pertinent SOPs and EOPs are followed, as well as all safety guidelines/protocols. Just because it is not human tissue doesn't mean that it can't have its share of nasties. Joe Saby Sent from Yahoo Mail on Android On Thu, Apr 16, 2020 at 8:21 AM, Porter, Amy via Histonet wrote: Make sure of insurance coverage and safety for the employee and that they are covered in case of injury - are they still clocking in and out in some fashion. just thinking in a bigger box. From: Steven Crochiere via Histonet Sent: Thursday, April 16, 2020 6:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Microtome at home Jaime, I don't see a problem with a research setting. If it was patient care, CLIA would need to inspect the set up in the person home. The same goes for our pathologists who read slide at home. Steve -Original Message- From: raestask via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Wednesday, April 15, 2020 7:51 PM To: Jamie Watson ; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Microtome at home I wouldn't think there would be any problem.Rae Staskiewicz HT(ASCP)Sent from my Verizon, Samsung Galaxy smartphone Original message From: Jamie Watson via Histonet Date: 4/15/20 6:44 PM (GMT-06:00) To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome at home Hello all,Our pathologist has come up with the idea of sending a microtome and waterbath home to someone that cannot come to work due to COVID 19. We are a research lab and work with mouse and rat tissue. Does anyone know of any issues with doing this? I have never heard of anyone cutting slides at home other than someone with a private business.Thank you.Jamie___Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!nwH0INyWP6lMeJ_Devv2eaelHE25_36kcLQnnnBFaO46Y8_BkxEnT0U_DaplXA$ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!nwH0INyWP6lMeJ_Devv2eaelHE25_36kcLQnnnBFaO46Y8_BkxEnT0U_DaplXA$ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] FW: Microtome at home
Make sure of insurance coverage and safety for the employee and that they are covered in case of injury - are they still clocking in and out in some fashion. just thinking in a bigger box. From: Steven Crochiere via Histonet Sent: Thursday, April 16, 2020 6:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Microtome at home Jaime, I don't see a problem with a research setting. If it was patient care, CLIA would need to inspect the set up in the person home. The same goes for our pathologists who read slide at home. Steve -Original Message- From: raestask via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Wednesday, April 15, 2020 7:51 PM To: Jamie Watson ; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Microtome at home I wouldn't think there would be any problem.Rae Staskiewicz HT(ASCP)Sent from my Verizon, Samsung Galaxy smartphone Original message From: Jamie Watson via Histonet Date: 4/15/20 6:44 PM (GMT-06:00) To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome at home Hello all,Our pathologist has come up with the idea of sending a microtome and waterbath home to someone that cannot come to work due to COVID 19. We are a research lab and work with mouse and rat tissue. Does anyone know of any issues with doing this? I have never heard of anyone cutting slides at home other than someone with a private business.Thank you.Jamie___Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!nwH0INyWP6lMeJ_Devv2eaelHE25_36kcLQnnnBFaO46Y8_BkxEnT0U_DaplXA$ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!nwH0INyWP6lMeJ_Devv2eaelHE25_36kcLQnnnBFaO46Y8_BkxEnT0U_DaplXA$ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: Microtome at home
Jaime, I don't see a problem with a research setting. If it was patient care, CLIA would need to inspect the set up in the person home. The same goes for our pathologists who read slide at home. Steve -Original Message- From: raestask via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Wednesday, April 15, 2020 7:51 PM To: Jamie Watson ; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Microtome at home I wouldn't think there would be any problem.Rae Staskiewicz HT(ASCP)Sent from my Verizon, Samsung Galaxy smartphone Original message From: Jamie Watson via Histonet Date: 4/15/20 6:44 PM (GMT-06:00) To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome at home Hello all,Our pathologist has come up with the idea of sending a microtome and waterbath home to someone that cannot come to work due to COVID 19. We are a research lab and work with mouse and rat tissue. Does anyone know of any issues with doing this? I have never heard of anyone cutting slides at home other than someone with a private business.Thank you.Jamie___Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
