[Histonet] New Dermatopath lab in Las Vegas

[Douglas Thomas via Histonet]

Hi,
My name is Doug Thomas MD of Thomas Dermatology in Las Vegas, NV.  We are in 
the process of building a dermatopathology lab.  We are looking to hire a 
couple of histology technicians and possibly a laboratory assistant.  Please 
email me if you are interested at 
dtho...@thomasderm.com.
Thank you,
dt

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Re: [Histonet] Warm formalin

[Lynette Pavelich via Histonet]

(Instructions For Use) The paperwork that you receive with each 
reagent/solution/stain, etc. 

> On Jul 22, 2020, at 3:10 PM, Muhammad Azam  wrote:
> 
> And IFU would be what 
> 
> Sent from my iPhone
> 
>> On Jul 22, 2020, at 3:02 PM, Lynette Pavelich via Histonet 
>>  wrote:
>> 
>> Carl,
>> My only other thought is to do a documented study of the temperature of 
>> these outside storage boxes (they can be little hot/cold boxes depending on 
>> your location. Because, according to the inspectors, you must be able to 
>> assure temperature is within the required temps prior to receiving, 
>> including transportation. (Pre-analytical)
>> So, say you do a study of the temperatures during the hottest time of the 
>> day of the boxes for a period of time (30 days of the hottest month(s)) to 
>> give an indication of a potential problem, and document. That would be one 
>> portion of pre-analytical. 
>> 
>> Then temperature during transportation (cars/vans can get quite hot/cold 
>> also needs to be addressed and documented daily just like the temperature 
>> logs in the labs. Many labs are getting cited for not providing good 
>> pre-analytical evidence. Courier services have to have temperature 
>> monitoring systems in place to provide as evidence. Some are quite savvy 
>> with alarm systems in place with monitoring. (ShipCom for example)
>> 
>> So much documentation these days……
>> 
>> Lynette
>> 
>> 
>>> On Jul 22, 2020, at 2:27 PM, Hobbs, Carl  wrote:
>>> 
>>> You a re right, Lynette
>>> De rigueur for Diagnostic labs!
>>> My apologies for forgetting that ( I am now in research labs where...it is 
>>> less restricted, unfortunately).
>>> Essential to be well-documented/adherent to SOPs.
>>> Respectful-illy
>>> 
>>> Carl
>>> 
>>> 
>>> Carl Hobbs FIBMS
>>> Histology and Imaging Manager
>>> Wolfson CARD
>>> Guys Campus, London Bridge 
>>> Kings College London
>>> London
>>> SE1 1UL
>>> 
>>> 
>>> 020 7848 6813
>>> 
>>> 
>>> From: Lynette Pavelich 
>>> Sent: 22 July 2020 19:19
>>> To: Hobbs, Carl 
>>> Cc: histonet@lists.utsouthwestern.edu 
>>> Subject: Re: [Histonet] Warm formalin 
>>> 
>>> I would suggest to always refer to your reagent’s IFU insert. This will 
>>> advise at what temperature you should use/store. All inspectors (CAP, JC, 
>>> CLIA, etc.) will make you adhere to these specifications. 
>>> Unless you do a well documented validation study that goes outside of these 
>>> restrictions from the IFU that proves no patient harm, you honestly must go 
>>> by the IFU recommendations. This would apply to all of our 
>>> stains/reagents/solutions/antibodies.
>>> Our world is becoming more restrictive……
>>> 
>>> hope this helps,
>>> Lynette Pavelich, HT(ASCP), QIHC
>>> 
>>> 
> On Jul 22, 2020, at 1:55 PM, Hobbs, Carl via Histonet 
>  wrote:
 
 Depends on what you are doing with the sections.
 IHC or just dye -staining?
 Sure...too hot ( cooking) is not recommended, as stated
 Also stated is that high -temp fixation may also be used with no 
 deleterious effects as long as the fixation time is not extended.
 However, RT -ish even for a week won't be a problem...imho
 Needs must?
 
 
 
 Carl Hobbs FIBMS
 Histology and Imaging Manager
 Wolfson CARD
 Guys Campus, London Bridge 
 Kings College London
 London
 SE1 1UL
 
 
 020 7848 6813
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>> 
>> 
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Re: [Histonet] Warm formalin

[Muhammad Azam via Histonet]

And IFU would be what 

Sent from my iPhone

> On Jul 22, 2020, at 3:02 PM, Lynette Pavelich via Histonet 
>  wrote:
> 
> Carl,
> My only other thought is to do a documented study of the temperature of these 
> outside storage boxes (they can be little hot/cold boxes depending on your 
> location. Because, according to the inspectors, you must be able to assure 
> temperature is within the required temps prior to receiving, including 
> transportation. (Pre-analytical)
> So, say you do a study of the temperatures during the hottest time of the day 
> of the boxes for a period of time (30 days of the hottest month(s)) to give 
> an indication of a potential problem, and document. That would be one portion 
> of pre-analytical. 
> 
> Then temperature during transportation (cars/vans can get quite hot/cold also 
> needs to be addressed and documented daily just like the temperature logs in 
> the labs. Many labs are getting cited for not providing good pre-analytical 
> evidence. Courier services have to have temperature monitoring systems in 
> place to provide as evidence. Some are quite savvy with alarm systems in 
> place with monitoring. (ShipCom for example)
> 
> So much documentation these days……
> 
> Lynette
> 
> 
>> On Jul 22, 2020, at 2:27 PM, Hobbs, Carl  wrote:
>> 
>> You a re right, Lynette
>> De rigueur for Diagnostic labs!
>> My apologies for forgetting that ( I am now in research labs where...it is 
>> less restricted, unfortunately).
>> Essential to be well-documented/adherent to SOPs.
>> Respectful-illy
>> 
>> Carl
>> 
>> 
>> Carl Hobbs FIBMS
>> Histology and Imaging Manager
>> Wolfson CARD
>> Guys Campus, London Bridge 
>> Kings College London
>> London
>> SE1 1UL
>> 
>> 
>> 020 7848 6813
>> 
>> 
>> From: Lynette Pavelich 
>> Sent: 22 July 2020 19:19
>> To: Hobbs, Carl 
>> Cc: histonet@lists.utsouthwestern.edu 
>> Subject: Re: [Histonet] Warm formalin 
>> 
>> I would suggest to always refer to your reagent’s IFU insert. This will 
>> advise at what temperature you should use/store. All inspectors (CAP, JC, 
>> CLIA, etc.) will make you adhere to these specifications. 
>> Unless you do a well documented validation study that goes outside of these 
>> restrictions from the IFU that proves no patient harm, you honestly must go 
>> by the IFU recommendations. This would apply to all of our 
>> stains/reagents/solutions/antibodies.
>> Our world is becoming more restrictive……
>> 
>> hope this helps,
>> Lynette Pavelich, HT(ASCP), QIHC
>> 
>> 
 On Jul 22, 2020, at 1:55 PM, Hobbs, Carl via Histonet 
  wrote:
>>> 
>>> Depends on what you are doing with the sections.
>>> IHC or just dye -staining?
>>> Sure...too hot ( cooking) is not recommended, as stated
>>> Also stated is that high -temp fixation may also be used with no 
>>> deleterious effects as long as the fixation time is not extended.
>>> However, RT -ish even for a week won't be a problem...imho
>>> Needs must?
>>> 
>>> 
>>> 
>>> Carl Hobbs FIBMS
>>> Histology and Imaging Manager
>>> Wolfson CARD
>>> Guys Campus, London Bridge 
>>> Kings College London
>>> London
>>> SE1 1UL
>>> 
>>> 
>>> 020 7848 6813
>>> ___
>>> Histonet mailing list
>>> Histonet@lists.utsouthwestern.edu
>>> https://eur03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonetdata=01%7C01%7Ccarl.hobbs%40kcl.ac.uk%7C2915a105cfa5436de65e08d82e6bd68b%7C8370cf1416f34c16b83c724071654356%7C0sdata=YZGiBCOwM5NmjBuxHh%2B%2FuhltOh%2F0HxJs5Kb8FaurKQM%3Dreserved=0
> 
> 
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Re: [Histonet] Warm formalin

[Lynette Pavelich via Histonet]

Carl,
My only other thought is to do a documented study of the temperature of these 
outside storage boxes (they can be little hot/cold boxes depending on your 
location. Because, according to the inspectors, you must be able to assure 
temperature is within the required temps prior to receiving, including 
transportation. (Pre-analytical)
So, say you do a study of the temperatures during the hottest time of the day 
of the boxes for a period of time (30 days of the hottest month(s)) to give an 
indication of a potential problem, and document. That would be one portion of 
pre-analytical. 

Then temperature during transportation (cars/vans can get quite hot/cold also 
needs to be addressed and documented daily just like the temperature logs in 
the labs. Many labs are getting cited for not providing good pre-analytical 
evidence. Courier services have to have temperature monitoring systems in place 
to provide as evidence. Some are quite savvy with alarm systems in place with 
monitoring. (ShipCom for example)

So much documentation these days……

Lynette


> On Jul 22, 2020, at 2:27 PM, Hobbs, Carl  wrote:
> 
> You a re right, Lynette
> De rigueur for Diagnostic labs!
> My apologies for forgetting that ( I am now in research labs where...it is 
> less restricted, unfortunately).
> Essential to be well-documented/adherent to SOPs.
> Respectful-illy
> 
> Carl
> 
> 
> Carl Hobbs FIBMS
> Histology and Imaging Manager
> Wolfson CARD
> Guys Campus, London Bridge 
> Kings College London
> London
> SE1 1UL
>  
> 
> 020 7848 6813
> 
> 
> From: Lynette Pavelich 
> Sent: 22 July 2020 19:19
> To: Hobbs, Carl 
> Cc: histonet@lists.utsouthwestern.edu 
> Subject: Re: [Histonet] Warm formalin 
>  
> I would suggest to always refer to your reagent’s IFU insert. This will 
> advise at what temperature you should use/store. All inspectors (CAP, JC, 
> CLIA, etc.) will make you adhere to these specifications. 
> Unless you do a well documented validation study that goes outside of these 
> restrictions from the IFU that proves no patient harm, you honestly must go 
> by the IFU recommendations. This would apply to all of our 
> stains/reagents/solutions/antibodies.
> Our world is becoming more restrictive……
> 
> hope this helps,
> Lynette Pavelich, HT(ASCP), QIHC
> 
> 
>> On Jul 22, 2020, at 1:55 PM, Hobbs, Carl via Histonet 
>>  wrote:
>> 
>> Depends on what you are doing with the sections.
>> IHC or just dye -staining?
>> Sure...too hot ( cooking) is not recommended, as stated
>> Also stated is that high -temp fixation may also be used with no deleterious 
>> effects as long as the fixation time is not extended.
>> However, RT -ish even for a week won't be a problem...imho
>> Needs must?
>> 
>> 
>> 
>> Carl Hobbs FIBMS
>> Histology and Imaging Manager
>> Wolfson CARD
>> Guys Campus, London Bridge 
>> Kings College London
>> London
>> SE1 1UL
>> 
>> 
>> 020 7848 6813
>> ___
>> Histonet mailing list
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Re: [Histonet] Warm formalin

[Hobbs, Carl via Histonet]

You a re right, Lynette
De rigueur for Diagnostic labs!
My apologies for forgetting that ( I am now in research labs where...it is less 
restricted, unfortunately).
Essential to be well-documented/adherent to SOPs.
Respectful-illy

Carl


Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge 
Kings College London
London
SE1 1UL
 

020 7848 6813


From: Lynette Pavelich 
Sent: 22 July 2020 19:19
To: Hobbs, Carl 
Cc: histonet@lists.utsouthwestern.edu 
Subject: Re: [Histonet] Warm formalin 
 
I would suggest to always refer to your reagent’s IFU insert. This will advise 
at what temperature you should use/store. All inspectors (CAP, JC, CLIA, etc.) 
will make you adhere to these specifications. 
Unless you do a well documented validation study that goes outside of these 
restrictions from the IFU that proves no patient harm, you honestly must go by 
the IFU recommendations. This would apply to all of our 
stains/reagents/solutions/antibodies.
Our world is becoming more restrictive……

hope this helps,
Lynette Pavelich, HT(ASCP), QIHC


> On Jul 22, 2020, at 1:55 PM, Hobbs, Carl via Histonet 
>  wrote:
> 
> Depends on what you are doing with the sections.
> IHC or just dye -staining?
> Sure...too hot ( cooking) is not recommended, as stated
> Also stated is that high -temp fixation may also be used with no deleterious 
> effects as long as the fixation time is not extended.
> However, RT -ish even for a week won't be a problem...imho
> Needs must?
> 
> 
> 
> Carl Hobbs FIBMS
> Histology and Imaging Manager
> Wolfson CARD
> Guys Campus, London Bridge 
> Kings College London
> London
> SE1 1UL
>  
> 
> 020 7848 6813
> ___
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Re: [Histonet] Warm formalin

[Lynette Pavelich via Histonet]

I would suggest to always refer to your reagent’s IFU insert. This will advise 
at what temperature you should use/store. All inspectors (CAP, JC, CLIA, etc.) 
will make you adhere to these specifications. 
Unless you do a well documented validation study that goes outside of these 
restrictions from the IFU that proves no patient harm, you honestly must go by 
the IFU recommendations. This would apply to all of our 
stains/reagents/solutions/antibodies.
Our world is becoming more restrictive……

hope this helps,
Lynette Pavelich, HT(ASCP), QIHC


> On Jul 22, 2020, at 1:55 PM, Hobbs, Carl via Histonet 
>  wrote:
> 
> Depends on what you are doing with the sections.
> IHC or just dye -staining?
> Sure...too hot ( cooking) is not recommended, as stated
> Also stated is that high -temp fixation may also be used with no deleterious 
> effects as long as the fixation time is not extended.
> However, RT -ish even for a week won't be a problem...imho
> Needs must?
> 
> 
> 
> Carl Hobbs FIBMS
> Histology and Imaging Manager
> Wolfson CARD
> Guys Campus, London Bridge 
> Kings College London
> London
> SE1 1UL
>  
> 
> 020 7848 6813
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
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Re: [Histonet] Warm formalin

[Sheeder, Christopher via Histonet]

How hot are we talking about?
San Francisco hot or Phoenix hot?


Christopher Sheeder, HT(ASCP)CMQIHC
Histology Supervisor | Department of Laboratories
Seattle Children's Hospital
4800 Sand Point Way NE
Seattle, WA 98105
Office: 206-987-6259

christopher.shee...@seattlechildrens.org


-Original Message-
From: Martin, Erin 
Sent: Tuesday, July 21, 2020 6:14 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Warm formalin

Hello everyone!

We have a referring clinician that is concerned about leaving his specimens in 
an outdoor lockbox in the summer because the formalin will get hot.  I don't 
think that having some specimens in formalin in hot weather would cause any 
problems but I can't find any references one way or another.  Does anyone have 
any policies regarding this?

Thanks so much!


Erin Martin, Histology Supervisor

UCSF Dermatopathology & Oral Pathology Service

Phone: 415-3537248 | Fax: 415-353-7543


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Re: [Histonet] Warm formalin

[Hobbs, Carl via Histonet]

Depends on what you are doing with the sections.
IHC or just dye -staining?
Sure...too hot ( cooking) is not recommended, as stated
Also stated is that high -temp fixation may also be used with no deleterious 
effects as long as the fixation time is not extended.
However, RT -ish even for a week won't be a problem...imho
Needs must?



Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge 
Kings College London
London
SE1 1UL
 

020 7848 6813
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Re: [Histonet] Warm formalin

[Garrey Faller via Histonet]

I agree with Paula. I think the doc has a legitimate concern. 
Garrey

Sent from my iPhone

> On Jul 22, 2020, at 1:20 AM, Patpxs via Histonet 
>  wrote:
> 
> Hi Erin,
> 
> Often heat is applied to formalin to speed up fixation.   That said there is 
> probably a temperature point where it goes from fixing tissue to cooking it.  
> 
> Paula
> 
> Sent from my iPhone
> 
>> On Jul 21, 2020, at 6:14 PM, Martin, Erin via Histonet 
>>  wrote:
>> 
>> Hello everyone!
>> 
>> We have a referring clinician that is concerned about leaving his specimens 
>> in an outdoor lockbox in the summer because the formalin will get hot.  I 
>> don't think that having some specimens in formalin in hot weather would 
>> cause any problems but I can't find any references one way or another.  Does 
>> anyone have any policies regarding this?
>> 
>> Thanks so much!
>> 
>> 
>> Erin Martin, Histology Supervisor
>> 
>> UCSF Dermatopathology & Oral Pathology Service
>> 
>> Phone: 415-3537248 | Fax: 415-353-7543
>> 
>> 
>> CONFIDENTIALITY NOTICE: This email message, including any attachments, is 
>> for the sole use of the intended recipient(s) and may contain confidential, 
>> proprietary, and/or privileged information protected by law. If you are not 
>> the intended recipient, you may not use, copy, or distribute this email 
>> message or its attachments. If you believe you have received this email 
>> message in error, please contact the sender by reply email and destroy all 
>> copies of the original message.
>> 
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