[Histonet] Needle in a Haystack

[Bonnie Salyer via Histonet]

Do you have experience using the PELCO Biowave? Are you proficient at using
TEM and specimen prep techniques for TEM?  Do you have some Histology
experience? What about Cryo-EM? and Immuno EM? If you can answer yes to all
or most of these, you may be me my 'Needle in a Haystack' and I would love
to talk to you. Contact me at bonnie_sal...@tedpella.com
 

 

We have an amazing opportunity for a Sr. Life Sciences Product Specialist.
See our website for complete details
https://www.tedpella.com/company_html/Careers.aspx 

 

Did I mention we live in one of the most beautiful areas ever? Live and work
in Redding, California -- an outdoor adventurers paradise where you can
hike, bike, fish, camp, ski, snowshoe, boat, kayak in some of the most
beautiful areas you have ever seen. We are a big town with all of what you
need in a city, but no big city chaos or traffic, and affordable! Perfect
for those looking to live in a smaller community, with beautiful scenery,
and great people.    https://visitredding.com/

 

 

 

 

Bonnie Salyer, SPHR, SHRM-SCP

Human Resources Manager

Ted Pella, Inc.

bonnie_sal...@tedpella.com  

(530) 243-2200 x260 office

(530) 227-0114 cell

(530) 243-8278 fax

 

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Re: [Histonet] slide printers

[Rathborne, Toni via Histonet]

Jessica,

We did this successfully. At one time, our cassette printers (Leica IPC) were 
not interfaced either, but we were able to use Leica's software and enter the 
cassette identifiers, and that information was able to be saved in the 
bar-code, which also printed on the cassette. Patient names were not printed, 
only the accession number/letter.
We have the SlideMate Pro printers, and before they were interfaced, we were 
able to scan the barcode on our blocks and they would print the information 
obtained from there. The SlideMates were also able to be programmed so that the 
hospital name was printed at the bottom of each slide.
So it can be done, but only if your blocks are bar-coded.

Toni




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-Original Message-
From: P Sicurello via Histonet 
Sent: Friday, January 14, 2022 10:06 AM
To: Piche, Jessica 
Cc: Histonet 
Subject: Re: [Histonet] slide printers


*** This is an External Email ***

Hi Jessica,

Slide writers don't have to be linked to a LIS but without that you really 
don't save any time. All the information will be typed in manually.

On the plus side-you don't have to worry about trying to read someone's hand 
writing if you use a slide writer.

Sincerely,

Paula Sicurello

Sent from my iPhone

> On Jan 14, 2022, at 5:35 AM, Piche, Jessica via Histonet 
>  wrote:
>
> Good Morning,
>
> Happy Friday Everyone.
>
> I was wondering what people are using/recommending for slide printers at the 
> microtome? Do they all have to be connected to an LIS?
>
> Thank you,
>
> Jessica
>
> Jessica Piche, HT(ASCP)
> Histology Team Leader
> Waterbury Hospital Histology Laboratory
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Re: [Histonet] slide printers

[P Sicurello via Histonet]

Hi Jessica, 

Slide writers don't have to be linked to a LIS but without that you really 
don't save any time. All the information will be typed in manually.  

On the plus side-you don't have to worry about trying to read someone's hand 
writing if you use a slide writer.  

Sincerely,

Paula Sicurello

Sent from my iPhone

> On Jan 14, 2022, at 5:35 AM, Piche, Jessica via Histonet 
>  wrote:
> 
> Good Morning,
> 
> Happy Friday Everyone.
> 
> I was wondering what people are using/recommending for slide printers at the 
> microtome? Do they all have to be connected to an LIS?
> 
> Thank you,
> 
> Jessica
> 
> Jessica Piche, HT(ASCP)
> Histology Team Leader
> Waterbury Hospital Histology Laboratory
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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Re: [Histonet] microtome blades and wax

[Rathborne, Toni via Histonet]

Jessica,

What has Leica said about the problem? I have always found them to be 
responsive and able to provide suggestions. This would be especially true with 
a new instrument, still under warranty. We have never had any inconsistencies 
with our Autocut, which was purchased last spring.

Toni




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and/or confidential information protected by law. It is intended only for use 
by the named addressee(s). If you are not the intended recipient of this 
e-mail, any dissemination, distribution or copying of this e-mail and its 
attachments, if any, is strictly prohibited. If you have received this 
transmission in error, please immediately notify the sender by telephone or by 
reply e-mail, and permanently delete this e-mail and the attachments, if any, 
and destroy any printouts.

-Original Message-
From: Piche, Jessica via Histonet 
Sent: Friday, January 14, 2022 8:43 AM
To: Histonet 
Subject: [Histonet] microtome blades and wax


*** This is an External Email ***

Good Morning,

We have been using Leica Histocore Autocut microtomes for a few months and have 
been having inconsistency with cutting on them. We have tried so many different 
blades, high and low, and have tried changing the blade angle. Nothing is a 
perfect fix, and we feel like we are really having to work much harder to get a 
great section. We were wondering what everyone else uses. I was also wondering 
if the wax could be an issue. We use EM-400 for embedding. I'd appreciate 
anyone's thoughts.

Thank you and have a nice weekend!

Jessica

Jessica Piche, HT(ASCP)
Waterbury Hospital Histology Laboratory
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Re: [Histonet] microtome blades and wax

[P Sicurello via Histonet]

Hi Jessica,

Happy Friday. 

Do you use the same EM-400 paraffin for processing?  If you use different 
paraffins that might contribute to your sectioning problems.   

If the waxes are different the tissue and the rest of the block are not one 
continuous unit in the "eyes" of the knife. The waxes have different properties 
so they cut differently because the embedding wax does not infiltrate into the 
tissue but wraps around it like a blanket.  

I'm not sure if that's what your lab does but if it is I suggest using the same 
paraffin for processing and embedding. 

Sincerely,

Paula Sicurello

Sent from my iPhone

> On Jan 14, 2022, at 5:43 AM, Piche, Jessica via Histonet 
>  wrote:
> 
> Good Morning,
> 
> We have been using Leica Histocore Autocut microtomes for a few months and 
> have been having inconsistency with cutting on them. We have tried so many 
> different blades, high and low, and have tried changing the blade angle. 
> Nothing is a perfect fix, and we feel like we are really having to work much 
> harder to get a great section. We were wondering what everyone else uses. I 
> was also wondering if the wax could be an issue. We use EM-400 for embedding. 
> I'd appreciate anyone's thoughts.
> 
> Thank you and have a nice weekend!
> 
> Jessica
> 
> Jessica Piche, HT(ASCP)
> Waterbury Hospital Histology Laboratory
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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[Histonet] microtome blades and wax

[Piche, Jessica via Histonet]

Good Morning,

We have been using Leica Histocore Autocut microtomes for a few months and have 
been having inconsistency with cutting on them. We have tried so many different 
blades, high and low, and have tried changing the blade angle. Nothing is a 
perfect fix, and we feel like we are really having to work much harder to get a 
great section. We were wondering what everyone else uses. I was also wondering 
if the wax could be an issue. We use EM-400 for embedding. I'd appreciate 
anyone's thoughts.

Thank you and have a nice weekend!

Jessica

Jessica Piche, HT(ASCP)
Waterbury Hospital Histology Laboratory
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[Histonet] slide printers

[Piche, Jessica via Histonet]

Good Morning,

Happy Friday Everyone.

I was wondering what people are using/recommending for slide printers at the 
microtome? Do they all have to be connected to an LIS?

Thank you,

Jessica

Jessica Piche, HT(ASCP)
Histology Team Leader
Waterbury Hospital Histology Laboratory
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