Re: [Histonet] Faded H tissue section

[Akemi Allison via Histonet]

Hi Tony:
I actually gave that tip to Lee back in 1979 when he came to OHSU to give a 
seminar to the Oregon Histology Society.  I found out that when I was doing GMA 
and the pathologists didn’t like Gills Hematoxylin. They loved my PAS on GMA so 
I tried using 1% Periodic Acid before using Harris Hematoxylin for H’s on GMA 
and it turned out beautifully. Guess he shared my tip with the rest of our 
society. He and I became closer se friends and we shared several tips, 
including his Movat’s tips which he didn’t publish, but I shared them in 
Frieda’s 4th Ed.

Best,
Akemi Allison-Tacha, BS, HT/HTL (ASCP)

Sent from my iPhone

> On Nov 9, 2023, at 6:11 PM, jayalakshmy p.s via Histonet 
>  wrote:
> 
> Thanks, I'll check it out.
> 
>> On Fri, Nov 10, 2023, 6:58 AM Tony Henwood  wrote:
>> 
>> You could treat the decoverslipped section in 1% periodic acid (same as
>> used in the PAS technique)  for 30 minutes prior to H staining. This
>> might improve the H staining.
>> 
>> 
>> 
>> I believe Lee Luna suggested this but for the life of me, I can’t find the
>> reference!
>> 
>> 
>> 
>> Regards,
>> 
>> 
>> 
>> Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired)
>> 
>> Principal Scientist, the Children’s Hospital at Westmead (Retired)
>> 
>> Adjunct Fellow, School of Medicine, University of Western Sydney.
>> 
>> 
>> 
>> *From: *jayalakshmy p.s via Histonet 
>> *Sent: *Friday, 10 November 2023 3:43 AM
>> *To: *histonet@lists.utsouthwestern.edu
>> *Subject: *[Histonet] Faded H tissue section
>> 
>> 
>> 
>> Hello,
>> I would like to know how to effectively restain a faded H tissue section.
>> The color becomes dull when re stained. Somebody please advise.
>> Prof. Jayalakshmy. P S
>> ___
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> 
>> 
>> 
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Re: [Histonet] Faded H tissue section

[jayalakshmy p.s via Histonet]

Thanks, I'll check it out.

On Fri, Nov 10, 2023, 6:58 AM Tony Henwood  wrote:

> You could treat the decoverslipped section in 1% periodic acid (same as
> used in the PAS technique)  for 30 minutes prior to H staining. This
> might improve the H staining.
>
>
>
> I believe Lee Luna suggested this but for the life of me, I can’t find the
> reference!
>
>
>
> Regards,
>
>
>
> Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired)
>
> Principal Scientist, the Children’s Hospital at Westmead (Retired)
>
> Adjunct Fellow, School of Medicine, University of Western Sydney.
>
>
>
> *From: *jayalakshmy p.s via Histonet 
> *Sent: *Friday, 10 November 2023 3:43 AM
> *To: *histonet@lists.utsouthwestern.edu
> *Subject: *[Histonet] Faded H tissue section
>
>
>
> Hello,
> I would like to know how to effectively restain a faded H tissue section.
> The color becomes dull when re stained. Somebody please advise.
> Prof. Jayalakshmy. P S
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
___
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Re: [Histonet] Faded H tissue section

[Tony Henwood via Histonet]

You could treat the decoverslipped section in 1% periodic acid (same as used in 
the PAS technique)  for 30 minutes prior to H staining. This might improve 
the H staining.

I believe Lee Luna suggested this but for the life of me, I can’t find the 
reference!

Regards,

Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired)
Principal Scientist, the Children’s Hospital at Westmead (Retired)
Adjunct Fellow, School of Medicine, University of Western Sydney.

From: jayalakshmy p.s via Histonet
Sent: Friday, 10 November 2023 3:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Faded H tissue section

Hello,
I would like to know how to effectively restain a faded H tissue section.
The color becomes dull when re stained. Somebody please advise.
Prof. Jayalakshmy. P S
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Re: [Histonet] Faded H tissue section

[Amos Brooks via Histonet]

Hi,
Don't decolonize. Soak the coverslip off and let it sit in xylene to
make absolutely sure all the mounting media is off then simply re-stain it.
If there is any mounting media left on it will interfere with the staining.
Unless there's something wrong with either the section or the reagents, it
should work just fine.

Amos Brooks

Message: 6
Date: Thu, 9 Nov 2023 22:02:46 +0530
From: "jayalakshmy p.s" 
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Faded H tissue section
Message-ID:

Content-Type: text/plain; charset="UTF-8"

Hello,
I would like to know how to effectively restain a faded H tissue section.
The color becomes dull when re stained. Somebody please advise.
Prof. Jayalakshmy. P S
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[Histonet] Faded H tissue section

[jayalakshmy p.s via Histonet]

Hello,
I would like to know how to effectively restain a faded H tissue section.
The color becomes dull when re stained. Somebody please advise.
Prof. Jayalakshmy. P S
___
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