[Histonet] Knife sharpener Reichert-Jung (Leica SP9000)
Dear colleagues! Can anyone help me to solve the problem with the knife sharpener Reichert-Jung (Leica SP9000)? My unit is need to replace some parts. -- Sincerely, Russia, Taganrog, Patho-anatomical bureau, Maxim Peshkov, biologist. -- Пешков Максим Отправлено из Почты Mail.ru ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fwd[2]: Re: AFIP Manual
Dear Bob! Free download AFIP manuals: 4th ed: https://books.google.ru/books/about/Laboratory_methods_in_histotechnology.html?id=R1xrMAAJ_esc=y 3rd ed: https://archive.org/details/ManualHistologicalStaining/mode/2up?q=staining+methods+afip+manual 2nd ed: https://archive.org/details/manualofhistolog00arme/page/n1/mode/2up или https://books.google.ru/books?id=CFRBYAAJ=frontcover=ru=gbs_ge_summary_r=0#v=onepage=false 1st ed: https://books.google.ru/books?id=vx1rMAAJ=frontcover=ru=gbs_ge_summary_r=0#v=onepage=false Enjoy, please. Maxim Peshkov, Russia, Taganrog. -- Пешков Максим -- -- Пешков Максим -- -- Пешков Максим ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Article form old journal
Dear histonetters! Can anyone share with me a scan of article by Hinshaw, J. R. and Pierce, H. E. (Surg Gyn Obstet, 103:726-730, 1956) please? This article is not available in internet. Sincerely, Maxim Peshkov Russia, Taganrog. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] On-line references
Many old books are here as e-versions: www.archive.org -- Maxim Peshkov Russia Taganrog ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FISH for breast core (Her2)
Dear colleagues! Can anyone help? We are very appreciatated any suggestions, tips and hacks for Her2 FISH onto breast trepine fixed in 10% NBF. It was here for more than 7 days, then processed as usual into wax. IHC for Her2 has value as 2+. Sincerely, Maxim Peshkov, Russia, Taganrog. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Dear Histonetters! I need in your proffessional help. Can you explain for me a the chemical mechanism of Picro-Mallory V stain by chemcial language? I will appreciate references about this issue except original article of Lendrum A.C. et al (1962) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC480427/pdf/jclinpath00070-0009.pdf Before asking I was read some histotechnological books, but can not find it. They are: Bancroft (from 5 to 8th ed), F. Carson A Self-instructional text 3 ed, C.F.A. Culling (3rd ed), AFIP manuals (3-4 ed), Woods and Ellis (Histology lab: A complete reference), Lilli RD, 1962, Romeis 2014 (18 auflage) and some others book. -- Russia, Taganrog, Maxim Peshkov. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] decal protocol with EDTA for bone marrow biopsies
We fixed our BM biopsies at least 24 hours, but no more than 72 h in 10% NBF. Wash with tap water 30 mins. Decal into 14% EDTA (2Na salt) buffered with NaOH up to 7.0 overnight or weekend and test endpoint by weight loss weight gain method (special thanks to Gayle Callis!). Wash in tap water 1 hour. Process with isopropanols (IPA), mixtures IPA and mineral oil), paraffin wax. Microtomy at 3 microns. Stain HE, Giemsa, HC, IHC. -- Maxim Peshkov, Russia, Taganrog. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Refurbished Leica RM2135
From sugh expensive blades can be done more very good sections (at 3 microns) from any difficult type tissue. These investments will pay off very quickly. Maxim Peshkov Russia, Taganrog. >Вторник, 2 мая 2017, 21:16 +03:00 от "Howery, Jeff L" ><jeff.how...@kadlecmed.org>: > >But, high profile knives are more expensive. This is due to the increased >amount of Stainless steel needed to make them. > >-----Original Message----- >From: Пешков Максим via Histonet [mailto:histonet@lists.utsouthwestern.edu] >Sent: Tuesday, May 02, 2017 11:12 AM >To: Sandra Cheasty >Cc: histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] Refurbished Leica RM2135 > > >Sandra, high profile blades was better for me, because they are harder and >more durable than low profile of any manufacturer. Also I recommend for you to >try Surgipath DB80HS. They must better than 818. I regret that not having >bought a holder for a high profile blades. >Leica makes the blade holders only for one type blades (low or high). >Microm's blade holder made for both type blades. >-- >Sincerlely, >Maxim Pehkov >Russia, >Taganrog. > > >___ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Refurbished Leica RM2135
Sandra, high profile blades was better for me, because they are harder and more durable than low profile of any manufacturer. Also I recommend for you to try Surgipath DB80HS. They must better than 818. I regret that not having bought a holder for a high profile blades. Leica makes the blade holders only for one type blades (low or high). Microm's blade holder made for both type blades. -- Sincerlely, Maxim Pehkov Russia, Taganrog. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
