[Histonet] Xylene Substitutes
Adrienne, If you'd like information on Formula 83 please contact me off list at b...@cbgbiotech.com Thanks, Beth Sell CBG ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet- Xylene Substitutes
Vanessa, I work with a lot of great histology folks and what I know about it is that eosin bleeding and blue-haze problems usually occur as a consequence of either not fully removing all of the water during the final absolute alcohol rinse step, and/or not fully removing all of the alcohol during the clearing step. If the water and alcohol are completely removed, bleeding and haze will not occur. I hope this helps. Beth Austin-Sell CBG Biotech -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 80, Issue 4 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Re: control tissue for Hall's bile stain (Robert Richmond) 2. RE: BILLING QUESTION (Cynthia Pyse) 3. RE: ER and PR validation (jmye...@aol.com) 4. Thomas Crowell is out of the office. (thomas.crow...@novartis.com) 5. Looking for MIN6 cell line (Debra Baluch) 6. bat wing histology (Amos Brooks) 7. RE: BILLING QUESTION (Feher, Stephen) 8. xylene substitute (Vanessa Avalos) -- Message: 1 Date: Fri, 2 Jul 2010 13:48:52 -0400 From: Robert Richmond rsrichm...@gmail.com Subject: [Histonet] Re: control tissue for Hall's bile stain To: histonet@lists.utsouthwestern.edu Message-ID: aanlktildu0qipr9rjajgqqonoaanf2yk1skc0houk...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Hall's bile stain - not really a stain but a reaction, like the Perls prussian blue reaction for iron - uses trichloracetic acid and ferric chloride to oxidize yellow-brown bilirubin to bright green biliverdin. Or so I've been told - I've never seen one. Something I've wondered about for a long time: could Hall's reaction be used to demonstrate meconium in macrophages in the chorion of the human placenta? These can be hard to see, particularly if you don't have a very good microscope. - If this would work, it would provide a ready source for controls, at least for the hospital pathologist. Bob Richmond Samurai Pathologist Knoxville TN -- Message: 2 Date: Fri, 2 Jul 2010 13:51:51 -0400 From: Cynthia Pyse cp...@x-celllab.com Subject: RE: [Histonet] BILLING QUESTION To: 'Sara Baldwin/mhhcc.org' sbald...@mhhcc.org, histonet@lists.utsouthwestern.edu Message-ID: 01cb1a0f$40ca1c10$c25e54...@com Content-Type: text/plain; charset=us-ascii We have a contract with the hospitals, this comes from our billing manger. We bill the hospitals, then they bill the patient. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Friday, July 02, 2010 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BILLING QUESTION Histonetters: If you do some work for another hospital (Histology) can you bill the hospital or do you have to bill the patient directly? Is there a statute or Regulation out there about this? Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbald...@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 3 Date: Fri, 2 Jul 2010 13:54:26 EDT From: jmye...@aol.com Subject: RE: [Histonet] ER and PR validation To: histonet@lists.utsouthwestern.edu Cc: dorothy.l.w...@healthpartners.com Message-ID: 8f84.3cbd32f0.395f8...@aol.com Content-Type: text/plain; charset=US-ASCII This paper can also be downloaded, indirectly, from the CAP's website: _http://www.archivesofpathology.org/doi/pdf/10.1043/1543-2165-134.7.e48_ (http://www.archivesofpathology.org/doi/pdf/10.1043/1543-2165-134.7.e48) Happy (re-)validating, Joe -- Message: 12 Date: Fri, 2 Jul 2010 10:23:08 -0500 From: Webb, Dorothy L dorothy.l.w...@healthpartners.com Subject: [Histonet] ER and PR validation To: _'histonet@lists.utsouthwestern.edu'_ (mailto:'histonet@lists.utsouthwestern.edu') In a recent
[Histonet] RE: Idea for a new recycler
If formalin, xylene, hemo-de, Formula 83 and alcohol were all collected as one waste stream, no recycler could separate this waste into recycled formalin, recycled solvent (xylene/hemo-de/F83), and recycled alcohol all in one run due in part to the small range of boiling points and in part to the azeotropes formed between the various chemicals. In this particular scenario, the alcohol and the formalin would distill over together along with any water in the formalin, and there would be a considerable amount solvent in the alcohol making it contaminated and not of any use in the lab. If you have any questions, please contact us off site and we'll be happy to assist you. Best Regards, Elizabeth Sell CBG Biotech 1-800-941-9484 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 78, Issue 7 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. clo test (Tench, Bill) 2. IDEA FOR A NEW RECYCLER (Madary, Joseph) 3. RE: RE: Barcode and Tracking Information (Rae Staskiewicz) 4. CD 133 (Ingles Claire ) 5. Disposable blade holder - amateur microscopist lookingfor a cheap one! (Gordon Brown) 6. Disposable blade holder - amateur microscopist lookingfor a cheap one! (Gordon Brown) 7. NSH Region II Meeting-discount hotel rate deadline is coming up! (Goodwin, Diana) 8. dehydration of hydrated slide (Eva Permaul) 9. RE: dehydration of hydrated slide (Sebree Linda A) 10. RE: dehydration of hydrated slide (Mauger, Joanne) 11. B5 fixative (histot...@imagesbyhopper.com) 12. myocyte damage (Bartlett, Jeanine (CDC/OID/NCZVED)) 13. RE: Responses to IHC CAP Validation question (tonia.richm...@gracepathology.com) 14. Starting up new lab (Shaw, Sharon) 15. Formalin fixation time for breast specimens (Richard Cartun) 16. RE: Responses to IHC CAP Validation question (bsulli...@shorememorial.org) 17. Re: myocyte damage (Merced M Leiker) -- Message: 1 Date: Wed, 5 May 2010 13:57:20 -0700 From: Tench, Bill bill.te...@pph.org Subject: [Histonet] clo test To: histonet@lists.utsouthwestern.edu Message-ID: 2820431bf953bb4da3e9e1a5882265fd02863...@mail1.pph.local Content-Type: text/plain; charset=us-ascii The Clo test is a clinical lab test. You need to go to that part of the CPT coding book (sorry I don't have it available). 88300 is an anatomic code (gross only, ie, it requires examination of a piece of tissue or foreign body) and is entirely inappropriate for this test. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, May 05, 2010 1:36 PM To: histonet@lists.utsouthwestern.edu Subject: [BULK] Histonet Digest, Vol 78, Issue 6 Hi everyone, I am looking to see what CPT code everyone is using for reading Clo Tests in the pathology department. I have heard of using 87081 but I am not sure if this is accurate as this is for culture and the CLO is biochemical reaction not a culture. Currently I have been using 88300 gross only. Any help would be appreciated. Thank you, Amy Farnan *** mail2.pph.org made the following annotations - Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. - -- Message: 2 Date: Wed, 5 May 2010 17:22:22 -0400 From: Madary, Joseph mada...@medimmune.com Subject: [Histonet] IDEA FOR A
Re: [Histonet] hydrometer
CBG Biotech offers hydrometers. Regards, Beth Austin CBG Biotech 1-800-941-9484 ext 200 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 76, Issue 11 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Helicobacter Pylori antibody (Chakib Boussahmain) 2. RE: Helicobacter Pylori antibody (McMahon, Loralee A) 3. RE: Helicobacter Pylori antibody (Sebree Linda A) 4. Tissue Tek (ricky hachy) 5. RE: Helicobacter Pylori antibody (Cynthia Pyse) 6. Re: Helicobacter Pylori antibody (Kim Tournear) 7. Re: Eosin too pink (Kim Tournear) 8. hydrometer (Vickroy, Jim) 9. Re: hydrometer (dkb...@chs.net) 10. Region II mtg addiiton and up-date: (Barone, Carol ) 11. p16 (Knutson, Deanne) 12. Re: Butyl alcohol (Rene J Buesa) 13. Masson Trichrome, Alligator Heart Tissue, colors turning out purple (cscam...@uci.edu) 14. Need help with FISH staining protocol on frozen tissue sections (Shen, Jinhui) 15. Re: p16 (Dana Settembre) 16. RE: p16 (Cynthia Pyse) 17. Masson Trichrome, Alligator Heart Tissue, colors turning out purple (Durden, Kelley) 18. Microwave Tissue Processor Protocols (Green JumpyOne) 19. HistoDay (Amy Johnson) 20. Histology Professionals Day (Breeden, Sara) 21. Re: Histology Professionals Day (Nita Searcy) 22. Happy HPD to our Unsung Heroes (Alyssa Peterson) 23. Re: Need help with FISH staining protocol on frozen tissue sections (Merced M Leiker) 24. RE: Histology Professionals Day (Beckham, Sharon) 25. RE: HistoDay (Marsh, Nannette) 26. RE: HistoDay (Marsh, Nannette) 27. Histo Professionals Day (Breeden, Sara) -- Message: 1 Date: Tue, 9 Mar 2010 10:45:54 -0800 (PST) From: Chakib Boussahmain chak_...@yahoo.com Subject: [Histonet] Helicobacter Pylori antibody To: histonet@lists.utsouthwestern.edu Message-ID: 591938.93716...@web58105.mail.re3.yahoo.com Content-Type: text/plain; charset=iso-8859-1 Hey Histonet, Is anyone using Helicobacter pylori antibody? if so, can you tell me where did you get it? and the protocol? Any input will appreciated Thank you so much. Chakib HTL(ASCP) MIT-DCM -- Message: 2 Date: Tue, 9 Mar 2010 13:52:36 -0500 From: McMahon, Loralee A loralee_mcma...@urmc.rochester.edu Subject: RE: [Histonet] Helicobacter Pylori antibody To: Chakib Boussahmain chak_...@yahoo.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: c27aa2a01cef31469813089e226f582e63759...@urmcms7.urmc-sh.rochester.edu Content-Type: text/plain; charset=us-ascii I have ordered mine from Cell Marque. We use it at 1/50 dilution with a 30 minute incubation at RT on the Dako Autostainers. Works great. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Chakib Boussahmain [chak_...@yahoo.com] Sent: Tuesday, March 09, 2010 1:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Helicobacter Pylori antibody Hey Histonet, Is anyone using Helicobacter pylori antibody? if so, can you tell me where did you get it? and the protocol? Any input will appreciated Thank you so much. Chakib HTL(ASCP) MIT-DCM ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 3 Date: Tue, 9 Mar 2010 12:58:14 -0600 From: Sebree Linda A lseb...@uwhealth.org Subject: RE: [Histonet] Helicobacter Pylori antibody To: Chakib Boussahmain chak_...@yahoo.com, histonet@lists.utsouthwestern.edu Message-ID: 8c023b4ab999614ba4791baeb26e27381bf...@uwhc-mail01.uwhis.hosp.wisc.edu Content-Type: text/plain; charset=iso-8859-1 Hi Chakib, We use Ventana's (manufactured by Cell Marque) H. Pylori on their instruments. The protocol is specific to the instruments but involves a routine HIER step and Avidin/Biotin blocking. The antibody itself is ready-to-use but is available from Cell Marque
[Histonet] Formula 83
Mr. Richmond, I was contacted off site and asked to clarify something you posted to the Histonet. I hope you do not mind. The flashpoint of Formula 83 is not 7 degrees Fahrenheit. It is 7 degrees Celsius, or 45 degrees Fahrenheit. Also please note that the auto ignite temperature is 482 degrees Fahrenheit. Respectfully, Beth Austin-Sell CBG Biotech -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 75, Issue 8 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: GI, Uro, or Derm Path Lab Set Up (Sally Price) 2. Formula 83 (Robert Richmond) 3. RE: cleaning cryostat (Ingles Claire ) 4. What slides do you use? (Scott Hendricksen) 5. New slide drier recommendations please (Scott Hendricksen) 6. Re: BioCare immunostainer (Pat Laurie) 7. Slide baking before IHC (Pat Laurie) 8. Re: RE: GI, Uro, or Derm Path Lab Set Up (Jay Lundgren) 9. Temperature and Humidity Monitoring System (teri.hall...@midmichigan.org) 10. RE: Slide baking before IHC (Morken, Tim) 11. RE: Temperature and Humidity Monitoring System (Liz Chlipala) -- Message: 1 Date: Thu, 4 Feb 2010 19:48:23 -0500 From: Sally Price sprice2...@gmail.com Subject: [Histonet] RE: GI, Uro, or Derm Path Lab Set Up To: histonet@lists.utsouthwestern.edu Message-ID: ffd26fa81002041648y5e648596sc4034bf7e8437...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 ...and another thing -- since when is it OK to post such blatant solicitations on the HistoNet? other vendors are chastised for this on a regular basis! Sally -- Message: 2 Date: Thu, 4 Feb 2010 21:14:50 -0500 From: Robert Richmond rsrichm...@gmail.com Subject: [Histonet] Formula 83 To: Histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: abea52a61002041814g4e82823dx50ebc41d77dd8...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Allison Hutton, HTL(ASCP)cm, Lead Tech Histology, Doylestown Hospital, Doylestown, Pennsylvania asks: For those of you who are using Formula 83, could you please contact me off line? We have completed our demo but still have a few questions. Formula 83, offered by CBG Biotech Ltd., is a napthenic (cycloalkane) hydrocarbon used as a xylene substitute. It differs in structure from the aliphatic hydrocarbons such as Richard Allan's Clear-rite 3. It's in use in a lab I'm working in at the moment. I have two reservations about it. The first is that, unlike the aliphatics, it has a definite odor, which could be objectionable in a confined space. The second reservation: Formula 83 has a flash point of only 7 degrees Fahrenheit, lower than xylene (78 degrees) and other aliphatics, some of which have flash points as high as 144 F. The fire hazard here is worth considering. Formula 83 has been discussed before on Histonet, and the posts are worth looking up. When purchasing a new aliphatic, there are several important considerations. Your purchasing people need to understand that they can't change the xylene substitute on you, just because something a bit cheaper comes along. If you recycle, every different xylene substitute has a different distillation routine. It's important to ask everyone in the lab - in particular, the cytotechnologist is likely to have very strong opinions about the solvents they use in their ascending sequence before coverslipping. Bob Richmond Samurai Pathologist Knoxville TN -- Message: 3 Date: Thu, 4 Feb 2010 22:44:24 -0600 From: Ingles Claire cing...@uwhealth.org Subject: RE: [Histonet] cleaning cryostat To: histonet@lists.utsouthwestern.edu Message-ID: f2f030053f9b7345831bed293a6d57e109a...@uwhc-mail01.uwhis.hosp.wisc.edu Content-Type: text/plain;charset=iso-8859-1 I work in a Mohs Lab. (high output frozen skin) We wipe out the cryostat with 95% ETOH and clear out the shavings every day we use our machines. We normally tear down and completely defrost our cryostats every other month or so, depending on humidity
