[Histonet] Part 4 - Book and Atlases - Final

[Connolly, Brett M via Histonet]

Hi all,

Thanks for the tremendous response! I am delighted that 38 books and atlases 
have found new homes. I only have 2 left if there is still interest in these.  
Sorry - no more freebies available.


Primate Brain Maps: Structure of the Macaque Brain;   ISBN 0-444-50415-X   
$50:00

Atlas of UroSurgical Anatomy;   ISBN 0-7216-3955-0  $30.00


Best regards,
Brett
Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Part 3 - Book and Atlases they're going fast

[Connolly, Brett M via Histonet]

Hi all,

Thanks for all of your responses!  The list has shrunk considerably.

Here is what left.  I forgot to add the Sheehan & Hrapchak classic: Theory and 
Practice of Histotechnology (this one is a little worn---go figure).

Atlas of UroSurgical Anatomy;   ISBN 0-7216-3955-0  $30.00
Comparative Anatomy and Histology: A Mouse and Human atlas;   ISBN 
978-0-12-381361-9  $80.00
Human Histology 2nd Ed;   ISBN 0-7234-2485-3  $40.00
Immunochemistry (LabFax);  ISBN 1-872748-05-8   $12.00
Atlas of Normal Histology 6th Ed;   ISBN 0-8121-1126-5   $12.00
Primate Brain Maps: Structure of the Macaque Brain;   ISBN 0-444-50415-X   
$50:00
Structure of the Human Brain: A Photographic Atlas 3rd Ed:  ISBN 0-19-504357-X  
 $20.00
Theory and Practice of Histotechnology 2nd Ed;   ISBN 0-8016-4573-5  $50.00

Free books
A Textbook of Histology 10th Ed;   ISBN 0-7216-1757-3
The Cell: Its Organelles and Inclusions - Atlas of Fine Structure;  ISBN 
0-7216-3585-7
Ham' s Histology 9th Ed;  ISBN0-397-50681-3
Basic Histology 6th Ed:   ISBN 0-8385-0575-9
The Human Brain: An introduction to Its Functional Anatomy 5th Ed;  
ISBN0-323-01320-1
Animal Processing Manual 1st Ed; Nat. Soc for Histotechnology
Dictionary of Human Neuroanatomy   ISBN 3-540-66523-4



Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Books and Atlases- Part 2 (with pricing and more books added)

[Connolly, Brett M via Histonet]

Hi all,

Thanks for all the responses to my previous post.  I really want to pass these 
along rather than trash them.

I updated the list and included my asking price. In addition I have a second 
list of free books - provided you purchase a book or books (one free book per 
purchase).

I'll pay for shipping within the continental US.

Couldn't be easierplease help me find a new home for these. I realize some 
are older, but histology and anatomy hasn't changed much.  You can Google the 
ISBN# or copy and paste the ISBN# into Amazon Books ISBN locator for more info 
on these.

I think they priced fairly, but I can negotiate.

Here is the more recent list:
Short Protocols in Molecular Biology 4thEd;   ISBN 0-471-32938-X  $25.00
Antigen Retrieval Techniques: Immunohistochemistry and Molecular Morphology;   
ISBN 1-881299-43-0 SOLD
Diagnostic Immunohistochemistry 2nd Ed;   ISBN 0-443-06652-3  SOLD
Atlas of Dermatopathology;   ISBN 0-7216-4886-X  $50.00
Atlas of UroSurgical Anatomy;   ISBN 0-7216-3955-0  $30.00
Comparative Anatomy and Histology: A Mouse and Human atlas;   ISBN 
978-0-12-381361-9  $80.00
Human Histology 2nd Ed;   ISBN 0-7234-2485-3  $40.00
A Color Atlas of Histology;ISBN 0-673-99190-3  $10.00
Color Atlas of Histology 3rd Ed;  ISBN 0-683-30642-1  $10.00
Histology for Pathologists 2nd Ed;ISBN 0-397-51718-1  SOLD
Immunochemistry (LabFax);  ISBN1-872748-05-8   $12.00
Immunological Reagents & Solutions: A Laboratory Handbook ;   ISBN 
1-881299-29-5   $15.00
Atlas of Normal Histology 6th Ed;   ISBN 0-8121-1126-5   $12.00
Histologic Preparations: Common Problems and Their Solutions;   ISBN 
978-0-930304-95-9  $50.00
Analytical Morphology: Theory, Applications & Protocols (soft cover);   ISBN 
1-881299-03-1   $15.00
A Color Atlas of Sectional Anatomy of the Mouse;   ISBN 4- 900659-58-4  $50.00
Gray's Anatomy 13th Ed;   ISBN 0-8121-0644-X$15.00
Primate Brain Maps: Structure of the Macaque Brain;   ISBN 0-444-50415-X   
$50:00
At Atlas of Primate Gross Anatomy: Baboon, Chimpanzee, and Man   ISBN  
0-89874-321-4  $50.00
Color Atlas of Nonhuman Primate Histology;   ISBN 3-8055-6879-7  $25.00
Structure of the Human Brain: A Photographic Atlas 3rd Ed:  ISBN 0-19-504357-X  
 $20.00
Atlas of the Rabbit Brain and Spinal Cord;  ISBN 3-8055-3814-6  $40.00
Laboratory Methods in Histotechnology (AFIP- red cover);   ISBN 1-881041-00-X  
$40.00

Here is the free book list:

A Textbook of Histology 10th Ed;   ISBN 0-7216-1757-3
Essential Immunology 6th Ed;   ISBN 0-632-01994-8
Antibody Applications: Essential Techniques;   ISBN 0-471-95698-8
Protein Localization by Fluorescence Microscopy: A Practical Approach;   ISBN 
0-19-963741-5
Techniques in Immunocytochemistry, Vol. 2;   ISBN 0-12-140405-6
Tissue In Situ Hybridization: Methods in Animal Development;   ISBN 
0-471-16403-8
The Cell: Its Organelles and Inclusions - Atlas of Fine Structure;  ISBN 
0-7216-3585-7
Ham' s Histology 9th Ed;  ISBN0-397-50681-3
Basic Histology 6th Ed:   ISBN 0-8385-0575-9
The Human Brain: An introduction to Its Functional Anatomy 5th Ed;  
ISBN0-323-01320-1
Gross Pathology: A Color Atlas;   ISBN 19-519797-6
Color Atlas of Histopathology;  ISBN 0-19-519151-X
Animal Processing Manual 1st Ed; Nat. Soc for Histotechnology
Dictionary of Human Neuroanatomy   ISBN 3-540-66523-4


Thanks,
Brett


Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] JOH journal collection --it's claimed

[Connolly, Brett M via Histonet]

All gone.
Brett

Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] JOH journal collection

[Connolly, Brett M via Histonet]

HI all,

Is anyone interested in old issues of JOH? My collection spans  1997 - 2016. I 
am missing a few (~ 7).

Let me know and I'll send you a list of what I have.

Brett

Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Thanks! CD3 antibody for mouse IHC

[Connolly, Brett M via Histonet]

Thanks to all who replied to my CD3 query! The responses were just what I was 
hoping for.

Best regards,
Brett

Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] CD3 antibody for FFPE mouse tissue

[Connolly, Brett M via Histonet]

Hi all,

Looking for recommendation for an anti-mouse CD3 antibody for FFPE sections.

I have some old Histonet messages referring to a Neomarkers RB-360 ab which I 
found through Thermo, but I would like to hear about more recent 
experiences/recommendations

Thanks as always,
Brett

Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] HSV-TK antibody for IHC

[Connolly, Brett M via Histonet]

Anyone know of an anti-HSV tyrosine kinase antibody suitable for IHC?  The ones 
I am finding are WB and ELISA only.

Thanks,
Brett

Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] HT Exam

[Connolly, Brett M via Histonet]

For $30.00 you can get them from ASCPprobably a good investment !!

http://www.starttest.com/7.2.0.0/cart.aspx?program=ASCPPractice

Brett

Brett M. Connolly, Ph.D.
Prin. Scientist, 
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803




-Original Message-
From: Kristy Castillo via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, March 29, 2016 8:41 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] HT Exam

How would I be able to get a example test for the HT exam?  Is there a place 
that you can go to get example questions?  Know someone studying for the exam, 
but she is not sure what to study and is really nervous.   Thanks Histonetters!

Kristy Castillo


This transmission may contain confidential information, some or all of which 
may be protected health information as defined by the federal Health Insurance 
Portability & Accountability Act (HIPAA) Privacy Rule. This transmission is 
intended for the exclusive use of the individual or entity to whom it is 
addressed and may contain information that is proprietary, privileged, 
confidential and/or exempt from disclosure under applicable law. If you are not 
the intended recipient (or an employee or agent responsible for delivering this 
transmission to the intended recipient), you are hereby notified that any 
disclosure, dissemination, distribution or copying of this information is 
strictly prohibited and may be subject to legal restriction or sanction. Please 
notify the sender by telephone to arrange the return or destruction of the 
information and all copies.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Masson's Trichrome or Sirius Red on frozen tissue

[Connolly, Brett M via Histonet]

Has anyone preformed these stains of frozen sections??? Looking for any tips.

I'll have fresh frozen tissue that can be fixed after sectioning and mounting 
onto the slide.

Thanks,
Brett

Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Lab Coats and OCT

[Connolly, Brett M via Histonet]

I am a fan of Cryo-Gel - highly viscous which facilitates easier tissue 
orientation. We get it from Electron Microscopy Sciences (#62806-01).
Try it, you'll like it.

Brett

Brett M. Connolly, Ph.D.
Prin. Scientist, 
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803



-Original Message-
From: Seeley, Heather via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, February 18, 2016 11:16 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Lab Coats and OCT

Hello All,





Now that the hospital is enforcing us to wear lab coats all the time, wondering 
if anyone has suggestions for some that aren't too hot, the ones we have now 
are making us sweat!



Also, we have been having issues with our OCT, we currently use the Sakura OCT 
and have use it for years. Within the last 6 months or so it has become like 
glue on the chucks even if we let it soak in hot water and scrub with a brush, 
it just doesn't want to come out! If anyone has any suggestions on brands to  
try we would appreciate it!

Thanks!


HEATHER SEELEY, HT(ASCP)
Histotechnologist
803-985-4676 OFFICE
803-327-7598 FAX

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] PDGFR beta antibody for IHC

[Connolly, Brett M via Histonet]

Anyone have a favorite for rodent FFPE tissue?

Thanks,
Brett

Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] DIF on paraffin embedded tissue

[Connolly, Brett M via Histonet]

Also agree. Lots of people do it. Search PubMed, J. Histo Cyto.

If you have the appropriate filters, using Cy dye secondary's can help move 
away from any autofluorescence.

Brett

Brett M. Connolly, Ph.D.
Prin. Scientist, 
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803




-Original Message-
From: Marcus Green via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, November 25, 2015 12:23 PM
To: Elizabeth Chlipala; Simmons, Christopher; Rene J Buesa
Cc: 'histonet@lists.utsouthwestern.edu' (histonet@lists.utsouthwestern.edu)
Subject: Re: [Histonet] DIF on paraffin embedded tissue


I, agreeing with Liz,

would suggest looking out a IF protocol for FFPE tissues - I've recently 
concluded a validation of PLA (proximity ligation assay) in: cells in culture, 
FFPE cell pellets and FFPE xenografts. The signal was consistently there 
(although a drop in expression - from fixative reasons as well as culture 
conditions). Background is the biggest issue for IF using FFPE so make sure to 
run some comprehensive controls.

You will need to run a Deparafinisation, Dehydration and Rehydration as you 
would IHC (3mins xylene x2, 3mins 100% EtOH, 3mins 70%EtOH and 3mins 50%EtOH - 
or similar) before running an Antigen Retrieval - if you have this optimised 
for IHC use something similar (pH and time). I would also consider a 
permeabilization step and if you need to optimise for background (Sudan black 
wash).

If, however the question is can you reinstate a tissue back to an unfixed 
tissue for freeze processing then no you can't.

hope this helps,
kind regards,

Marcus


From: Elizabeth Chlipala via Histonet [histonet@lists.utsouthwestern.edu]
Sent: 25 November 2015 16:53
To: Simmons, Christopher; Rene J Buesa
Cc: 'histonet@lists.utsouthwestern.edu' (histonet@lists.utsouthwestern.edu)
Subject: Re: [Histonet] DIF on paraffin embedded tissue

I'm not sure that is the case in the grand scheme of things, it will depend 
upon the target that you need to stain via immunofluorescence.  Technically we 
perform IF staining on frozen sections primarily because the antigen does not 
survive formalin fixation.  Many people utilize IF techniques on FFPE tissue 
with good success, we have done it here.  There are things you will need to 
consider and what I suggest below may not work at all.  It's up to you if you 
want to try it or not and you may feel it is not worth the time and energy 
required to see if it may work on FFPE samples, it could be a lot of work and 
it may not be successful.

1.  You will likely not be able to use your current protocol
2.  You will likely need to add an antigen retrieval step
3.  You may need to look for a different antibody source (one that survives 
formalin fixation)
4.  The signal needs to be good since formalin fixation will cause 
autofluorescence

Unless I am completely missing something here since two individuals have stated 
that the sample is useless, maybe there is a better explanation as to why the 
sample is completely useless.  Here is where I am coming from - technically you 
can perform IF staining on FFPE tissue samples, people do it all of the time, 
we have done it here, it's a common technique for dual labeling of samples when 
co-localization is an expected result.

Good Luck

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

-Original Message-
From: Simmons, Christopher via Histonet 
[mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, November 25, 2015 9:10 AM
To: Rene J Buesa
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] DIF on paraffin embedded tissue

Useless sample
Sorry

Sent from my iPhone

> On Nov 25, 2015, at 11:02 AM, Rene J Buesa via Histonet 
>  wrote:
>
> No matter WHO to tell you to do WHAT, for IF purposes, that FFPE
> tissue is USELESS.René
>
>
>On Wednesday, November 25, 2015 10:55 AM, Maryann Deathridge via Histonet 
>  wrote:
>
>
> We have a tissue sample that was processed and paraffin embedded.  We
> URGENTLY need to recover the tissue and perform Immunofluorescence on
> the sample.
> Does anyone have a procedure.  HELP
>
> madeathri...@pastnashville.com
>
>
>
>
>
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] CD8 IHC on FFPE mouse tissue -what your experience?

[Connolly, Brett M via Histonet]

Hi all,
I have a project that will be looking at CD8 expression in some FFPE murine 
tissue.  Past discussions here have mentioned the 14-0808 CD8a antibody from 
eBiosicence.

For those of you have had the opportunity to use this antibody I would like to 
hear your experiences/ recommendations about the staining results.

I'll need to do some quantitation of the staining so I am hoping this antibody 
is strong and specific.

Thanks much,
Brett

Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] autoradiography frozen human brain sections fall off, white matter issue

[Connolly, Brett M via Histonet]

Hi Esther,

We do a lot of autoradiography on rodent, primate and human brains sections. 
Our protocol is similar to yours except we do not dry the slides after 
incubating in the buffer and before adding the radiotracer.

The experiment is performed with the slides in a rack and totally immersed in 
the solutions in staining dishes.

1. incubate slides in assay buffer 15 min.  at RT
2. add radiotracer to buffer, gently mix and incubate 90-120 min. at RT
3. wash  GENTLY (no agitation) in ice-cold wash buffer 3 x 3 min. We set up 3 
containers and transfer the slides.
4. wash in ice-cold DI water 5 seconds.
5. air dry

The Superfrost gold slides are supposed to improve adherence and would be worth 
a try. It could be your other Superfrost slides are old or a bad batch...which 
I think has been experienced by some people on the list.

We do store our sectioned slides at -70C until use and then bring them up to RT 
the day of the experiment. They are sectioned, dried at RT for 15-20 min, and 
then store at -70C... No drying in the fridge.

Good luck,
Brett


Brett M. Connolly, Ph.D.
Principle Scientist, 
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

-Original Message-
From: Kooijman, Esther via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, October 02, 2015 5:20 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] autoradiography frozen human brain sections fall off, white 
matter issue
Importance: High

Dear Histonetters,

Does anyone of you have experience in autoradiography and could help me with 
the problem falling of tissue  ? Or suggestions ?

We have problems with falling off sections of human brain tissue.
We have done the same experiments and cutting in rodent tissue without any 
problems. Our protocol for autoradiography  :
Snap frozen tissue, cut in cryo 20um sections on Superfrost plus glass, drying 
ON in the fridge on silica.
Stored in -20.
Day of the experiment.
Getting them on room temperature about 45 minutes, washing/dehydrate on room 
temperature in 5mM Tris (HCL PH7.4) buffer, 20 minutes.
About  30 minutes - an hour drying at room temperature.
Then incubation with the radio tracer for 30 minutes, room temperature, just 
dripping the 1 mL solution to completely cover the superfrost plus glass and 
ditto tissue.
Turning the glass to get the solution of the glass,  dipping in ice cold tris 
washing buffer 5mM (HCL PH7.4) 1.5 minutes and here the disaster starts with 
falling off extensive white matter falling off.. its washing steps of only 
1.5 minute.

What can I do to prevent this, any idea ? I am out of clues. I have tried to 
dry the sections longer after cutting sections, I have tried to pre wash in 
either room temp or cold buffer. Hope someone can help me.
Would Superfrost plus gold glass be better ?

Kind Regards,

Esther Kooijman
Research Technician
VU University Medical Center
Nuclear Medicine & PET Research
Email: e.kooij...@vumc.nl
Amsterdam
The Netherlands

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Tissue request....

[Connolly, Brett M via Histonet]

Rachel,

If you have funding to purchase you can check out these biorepositories...

Asterand Bioscience
http://www.asterandbio.com/

National Disease Research Interchange
http://ndriresource.org/

Cooperative Human Tissue Network
http://www.chtn.nci.nih.gov/

Analytical Biological Services
http://www.absbio.com/

Cureline Laboratories
http://www.cureline.com/


Brett M. Connolly, Ph.D.
Principle Scientist, Imaging Dept.
Translational Biomarkers
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803





From: Rachel M Gonzalez via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Friday, September 18, 2015 7:19 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue request

Hi

Looking for control tissue for reviewing antibody specificity this is for
R project. I do not know if that will make a difference in the tissue
availability but I thought I put that out there.  If you know a good tissue
source can you let me know.

I need three of each type

normal bone marrow (hematopoietic cell and lymphocytes, and etc.),

normal brain/cerebrum,

normal cerebellum,

normal parathyroid glands,

normal hypophysis/Pituitary Gland

normal thymus.



Thank you in advance for help.

Rachel Gonzalez PhD

Senior Scientist
.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Acetone fixation problems with OCT Tissues

[Connolly, Brett M]

Patrick,

We do a lot of frozen section IHC work. Years ago Gayle Callis turned me on to 
fixing in cold acetone:ethanol (3:1) . We keep it at -20C and I fix for 10  
min. on the bench then wash in PBS and proceed with the IHC. We do dry slides 
for at least 30 min before fixing.  This has worked well in our hands for many 
different antibodies.

Brett

Brett M. Connolly, Ph.D.
Principle Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick
Sent: Tuesday, April 28, 2015 5:56 PM
To: (Histonet@lists.utsouthwestern.edu)
Subject: [Histonet] Acetone fixation problems with OCT Tissues


Hi Everyone,

I am still having issues with my IHCs with Acetone fixation.

If I fix in 100% Acetone, I get IHC staining, but my tissues are 50-90% 
destroyed.

If I fix in 4% paraformaldehyde, or 10% NBF or (95%  Etoh and/or Methanol with 
Acetone) I lose the epitopes I either get no staining or very  weak staining, 
but the tissue morphology look fine.

I just tried an acetone gradient where I cut the tissues at 5 uM and dried them 
overnight, then fixed for 10 minutes in 100% acetone, then fixed in 95% acetone 
for 1 minute, then fixed in 70% acetone for 30 seconds, then quick rinsed in 
H20, then washed as normal in DPBS pH 7.4.

I did 4 slides, 2 slides with one company's Charged slides ,and 2 slides with 
another company's charged slides.

One company's slides look completely destroyed, the others may turn out, it was 
hard to tell how much damage there was.  I'll know tomorrow when I finish 
staining and Hemotoxylin them.



Patrick Lewis
Research Associate II Bench
Seattle Childrens Research Institute
206-884-1115

CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for 
the sole use of the intended recipient(s) and may contain confidential and 
privileged information protected by law. Any unauthorized review, use, 
disclosure or distribution is prohibited. If you are not the intended 
recipient, please contact the sender by reply e-mail and destroy all copies of 
the original message.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Instructions for J of Histo free access to archives through Maney Publishing

[Connolly, Brett M]

Most of you probably know that NSH members have free access to the JOH 
archives, but you need to log-in to MY NSH and then register with Maney 
Publishing to get them.

 I think you can get access to some JOH articles through PubMed as a 
non-member, but not all of them.

Instructions for members are below .

Brett



NSH Members

First do this...

NSH members can review the current issue and archived issues of the Journal of 
Histotechnology online at the Maney Publishing website by logging into their 
MY NSH account.  Click here to access MY NSH  then select Membership 
Directory/JOH Onlinehttp://www.nsh.org/mynshtemp. You will need to login 
using your primary email address on file with NSH and your password to My NSH. 
If you do not have a password or have questions about your log in please 
contact the NSH Office, 443-535-4060 or email 
hi...@nsh.orgmailto:hi...@nsh.org.

Then do this...
First Time Visitor
To obtain your online access to Journal of Histotechnology please go to 
www.maneyonline.com/loi/hishttp://www.maneyonline.com/loi/his and register by 
clicking on 'register' link in the top right-hand corner of the screen.  Once 
you have registered, click on your name which will appear in the top right of 
the screen.  This will take you to your 'My account' area of Maney Online.  
From here, please click on 'Access token' from the left-hand menu, and input 
the following token: NSHaccess.  Click submit.  You can then click on 'Access 
entitlements' in the left-hand menu where you will see a link to the Journal of 
Histotechnology where will have full access to the journal content.  You only 
have to complete the token process once.  On future visits you simply need to 
click www.maneyonline.com/loi/hishttp://www.maneyonline.com/loi/his , sign-in 
with your registered email address and password, and you will continue to have 
access to the journal content.

Registered Visitor
Click www.maneyonline.com/loi/hishttp://www.maneyonline.com/loi/his , sign-in 
with your Maney Online registered email address and password, and you will 
continue to have access to the journal content.


Brett M. Connolly, Ph.D.
Principle Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.commailto:brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Looking for an article

[Connolly, Brett M]

I sent her the .pdf file I downloaded from Many Pub.

Brett


Brett M. Connolly, Ph.D.
Principle Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of John Kiernan
Sent: Wednesday, February 18, 2015 4:34 PM
To: Morken, Timothy; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Looking for an article

If you are a member of NSH you can also get a pdf for free by way of nsh.org. 
See email copied below.
John Kiernan
London, Canada
= = =

Dear All,
 
 
 
I am pleased to confirm that the login button for the Journal of 
Histotechnology Editorial Manager site is now working. 
 
You may need to clear your internet browser cache before you can use the 
button. Instructions on how to do this are available at
(http://www.wikihow.com/Clear-Your-Browser%27s-Cache; 
target=_blankhttp://www.wikihow.com/Clear-Your-Browser)
 
My apologies for the inconvenience,
 
 
 
With best wishes,
 
 
 
Rasheda Begum
 
Editorial Assistant
 
 
 
Maney Publishing, 1 Carlton House Terrace, London, SW1Y 5AF, United Kingdom

Tel: +44 (0)20 7451 7408 Fax: +44 (0)20 7451 7307
 
Email:
r.be...@maneypublishing.com
 
_

On 18/02/15, Morken, Timothy  timothy.mor...@ucsf.edu wrote:
 If you are a member of NSH you can get a pdf for free just by contacting the 
 staff at the NSH office. A great reason to join, if you have not already!
 
 Tim Morken
 Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
 UC San Francisco Medical Center
 San Francisco, CA
 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] 
 histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Geoff
 Sent: Wednesday, February 18, 2015 12:51 PM
 To: Fawn Bomar; histonet@lists.utsouthwestern.edu
 Subject: Re: [Histonet] Looking for an article
 
 My error, I sent a link to a similar article. The original is in The Journal 
 of Histotechnology. 2006;29(3):173-176 but my library does not have that 
 journal. Perhaps someone on the list has it.
 
 Geoff
 
 On 2/18/2015 2:48 PM, Fawn Bomar wrote:
  Hi all,
 
 
 
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] CD31 IHC on bone

[Connolly, Brett M]

Hi all,

A colleague is having problems staining CD31 on bone sections and is looking 
for recommendations for specific antibodies and antigen retrieval methods.
Can anyone help?

Thanks,
Brett

Brett M. Connolly, Ph.D.
Principle Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.commailto:brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] HELP

[Connolly, Brett M]

We also use the MCA711 antibody (at 1.0 ug/mL, 1 hr.), but with HIER in citrate 
buffer using a Biocare Decloaker pressure cooker and Biocare's rat-on mouse HRP 
polymer detection. We  get very nice staining with no background. I was never 
too impressed with ImPRESS.

Here are the basics :
- Deparaffinize and hydrate to dH20
- Perform HIER, cool for 20 min then wash in H20
- 3.0% H2O2 - 20 min
- Serum free block (Biocare Sniper) 30 min
- Incubate with MCA771 1 hr.
- Incubate with Biocare rat-on-Mouse kit (per instructions)
- DAB - 5 min.
 Washes are with PBS/0.1% Tween

Brett


Brett M. Connolly, Ph.D.
Principle Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth 
Chlipala
Sent: Thursday, November 06, 2014 12:41 PM
To: Hans B Snyder; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] HELP

Hans

We use the antibody from Serotec, it works quite nicely.  MCA711, proteinase K 
digestion,  rabbit anti-rat secondary and then Envision Rabbit (polymer).  We 
use this antibody at a 1:1200 dilution.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com

March 10, 2014 is Histotechnology Professionals Day

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hans B Snyder
Sent: Thursday, November 06, 2014 10:31 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] HELP

Hello All,

Does anyone have a protocol for the Anti-Neutrophil antibody [NIMP-R14]
(ab2557) for mouse tissue?  I have been trying to extinguish the background 
staining but cannot find the right combination.
​ We are doing this by hand. ​
 The recommended dilution for this antibody is 1:50 with HIER.   See used
protocol below.

So far I have tried:
1. concentrations 1:25, 1:50, 1:75, 1:100  - 1:50 gives very high background 
but good positive staining, 1:100 gives low background but not much positive 
staining.

2. HIER - 3, 5, 10,  20, minutes  @ 96C, 20 minutes at 60C  all give the same 
results - too much back ground

3. We have tried using the H2O2 before the primary and after, increasing the 
time from 30 minutes to 45 minutes.

4. Tried incubating in the primary for shorter time (20 minutes) and longer 
overnight at 4C.

5. Tried blocking using 3 different serums, first each one then combinations 
and longer times.

6. Tried cutting the DAB concentration in 1/2 then 1/3.


We have not tried enzyme or acid epitope retrieval yet.



*Does anyone have a working protocol or suggestions on what to try?*


​Thank you in advance.​





Procedure:

Deparaffinize

1.  Heat slides to 60C for 10 minutes (oven).

2.  Dry slides at room temp 5 minutes.

3.  Xylene 5 minutes.

4.  Xylene 5 minutes.

5.  100% ethanol 5 minutes (dehydration).

6.  100% ethanol 2 minutes (dehydration).

7.  95% ethanol for 2 minutes (rehydration).

8.  70% ethanol for 2 minutes (rehydration).

9.   Run in DI water for 10 minutes.



Antigen Retrieval

1.  Submerge in 0.01M Citrate Buffer (pH 6.0) at 96C for 5 minutes.

2.  Cold running water 5 minutes.



Staining

1.  Wash in PBS for 1 min.

2.  Permeabilize with 0.1% Triton X / 2.5% horse serum + 2.5% fetal
bovine serum for 2 hours.

3.  Incubate in primary antibody 30-45 minutes at in 2.5% horse serum
in PBS.

4.  Wash 3x in PBS/tween for 5 minutes each.

5.  Fresh 3.0% hydrogen peroxide (H2O2) 2x for 20 minutes each.

6.  Wash 3x PBS 2 minutes each.

7.  Incubate with anti-Rat Ig impress solution (according to vector’s
instructions) 45 minutes.

8.  Wash 3x in PBS for 5 minutes each.

9.  Prepare Impact DAB substrate (see insert).

10.  Using a light microscope, incubate sections with 50-100ul, until desired 
darkness occurs (5-30 seconds).

11.  Stop DAB reaction using deionized water.

12.  Wash in DH2O for 5 minutes

13.  Counterstain in Harris hematoxylin for 30 seconds.

14.  Wash in DH2O for 5 minutes.

15.  Dehydrate 2x in 95% alcohol for 30 seconds each.

16.  Dehydrate 3x in 100% alcohol for 2 minutes each.

17.  Clear 2x in xylene 5 minutes each.

18.  Mount coverslips using Leica mounting media.


Hans B Snyder
Histologistics
60 Prescott Street
Worcester, MA 01605
508-308-7800
h...@histologistics.com
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One 

[Histonet] RE: Ebola

[Connolly, Brett M]

Here is the CDC guidelines link for those interested

http://www.cdc.gov/vhf/ebola/


Brett M. Connolly, Ph.D.
Principle Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri Braud
Sent: Friday, October 10, 2014 9:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Ebola

My area has a large west African population and we have already
established a interim procedure based on the CDC's guidelines, and the
Philadelphia Dept of Health, Division of Disease control. We also have a
response committee that is actively reviewing this procedure to insure
that it is up to date and that all involved are educated on how to
handle their piece.  It includes: response for any patient presenting
with fever, patient interviews including travel history, assessment of
exposure risk, reporting, isolation and housing precautions for suspect
patients, transportation and handling of specimens, and heaven forbid,
morgue procedures.
Universal precautions are just not enough. 

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3676
Fax: 215-938-3874
*
-



CONFIDENTIALITY NOTICE:

This E-Mail is intended only for the use of the individual or entity to which
it was sent. It may contain information that is privileged and/or confidential,
and the use or disclosure of such information may also be restricted under 
applicable
federal and state law. If you received this communication in error, please do 
not
distribute any part of it or retain any copies, and delete the original E-Mail.
Please notify the sender of any error by E-Mail.

Thank you for your cooperation.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] More TGIF

[Connolly, Brett M]

Enjoy some histotechnologist logos..

http://www.zazzle.com/histotechnologist



Brett M. Connolly, Ph.D.
Principle Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.commailto:brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Ebola

[Connolly, Brett M]

I did my postdoc research at USAMRIID on Ebola virus pathogenesis. Our standard 
fixation protocol for Ebola, Lassa and other BSL4 pathogens was to let them fix 
in formalin for a minimum of 30 days. The containers of fixed tissue were not 
allowed out of the P4 containment lab until the 30 day limit was up. Even then 
we had disinfectant dunk tanks to pass the sealed containers through to get 
them out of the P4  lab.  You can't be too careful with that bugger...just 
sayin'

Brett

Brett M. Connolly, Ph.D.
Principle Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of James Watson
Sent: Thursday, October 09, 2014 3:52 PM
To: 'Weems, Joyce K.'; 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Ebola

Have it delivered in formalin and let it fix completely.



Formalin fixation eliminates the biologic hazard associated with transporting 
samples containing infectious EBO virus,  Thus, formalin 
fixation provides an adequate noninfectious specimen for EHF diagnosis, 
.

A quote from:

Long-Term Disease Surveillance in Bandundu Region, Democratic Republic of the 
Congo: A Model for Early Detection and Prevention of Ebola Hemorrhagic Fever 

Ethleen S. Lloyd, and  C. J. Peters, et al
 

+ Author Affiliations

Centers for Disease Control and Prevention, Atlanta, Georgia; World Health 
Organization and US Agency for International Development and Ministry of 
Health, Kinshasa, Hôpital Général de Référence de Kikwit, Kikwit, and Mosango 
Mission Hospital, Mosango, Democratic Republic of the Congo; Médecins sans 
Frontières—Belgium, Brussels, Belgium 
 

+ Author Notes

↵* Current affiliation: Institut de Médecine Tropicale, Antwerp, Belgium 
(M.A.B.); Médecins sans Frontières—Belgium, N'Djamena, Chad (E.V.); World 
Health Organization, Kinshasa, Democratic Republic of the Congo (J.K.). 

Reprints or correspondence: Dr. Pierre E. Rollin, Special Pathogens Branch, 
NCID/DVRD, Mailstop G-14, Centers for Disease Control and Prevention, 1600 
Clifton Road, Atlanta, GA 30333 (p...@cdc.gov).


James Watson HT  ASCP
GNF  Genomics Institute of the Novartis Research Foundation
Scientific Technical Leader II, Histology
Tel    858-332-4647
Fax   858-812-1915
jwat...@gnf.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Thursday, October 09, 2014 12:22 PM
To: 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Ebola

Handle with universal precautions as always is our emphasis.

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's 
Hospital and is intended for the sole use of the intended recipient(s).  It may 
contain information that is privileged and confidential.  Any unauthorized 
review, use, disclosure, or distribution is prohibited. If you are not the 
intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya
Sent: Thursday, October 09, 2014 3:04 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Ebola

Dare I ask?! Are any Pathology labs discussing what to do with 
specimens/precautions, etc. regarding a person with a potential Ebola infection?

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 
19603-0316 ph  610-378-2635 fax 610-898-5871
email: tanyaabb...@catholichealth.net

This electronic mail and any attached documents are intended solely for the 
named addressee(s) and contain confidential information. If you are not an 
addressee, or responsible for delivering this email to an addressee, you have 
received this email in error and are notified that reading, copying, or 
disclosing this email is prohibited. If you received this email in error, 
immediately reply to the sender and delete the message completely from your 
computer system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



This e-mail message (including any attachments) is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
If the reader of this message is not the intended recipient, you are hereby 
notified that any dissemination, distribution or copying of 

RE: [Histonet] Ebola

[Connolly, Brett M]

Actually it has been proven that formalin will inactivate Ebola, as well as 
other hemorrhagic fever viruses. 
Brett

Brett M. Connolly, Ph.D.
Principle Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Thursday, October 09, 2014 4:34 PM
To: Pam Marcum; Tanya Abbott
Cc: Histonet@Lists. Edu
Subject: RE: [Histonet] Ebola

Well said Pam, it is just assumed that formalin will eliminate the biohaz for 
Ebola, I doubt if that has been conclusively proven yet, remember we only 
discovered fairly recently that formalin fixation did not protect us from 
CJD

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
pru...@ihctech.net


 Date: Thu, 9 Oct 2014 20:26:16 +
 From: mucra...@comcast.net
 To: tanyaabb...@catholichealth.net
 Subject: Re: [Histonet] Ebola
 CC: histonet@lists.utsouthwestern.edu
 
 Take Brett's advise and use that as guidleine.  We don't know as much as we 
 should about these viruses.   Pam 
 
 - Original Message -
 
 From: Tanya Abbott tanyaabb...@catholichealth.net 
 To: Histonet histonet@lists.utsouthwestern.edu 
 Sent: Thursday, October 9, 2014 2:03:47 PM 
 Subject: [Histonet] Ebola 
 
 Dare I ask?! Are any Pathology labs discussing what to do with 
 specimens/precautions, etc. regarding a person with a potential Ebola 
 infection? 
 
 Tanya G. Abbott RT (CSMLS) 
 Manager Technologist, Histology/Cytology 
 St. Joseph Medical Center 
 Reading, PA 19603-0316 
 ph  610-378-2635 
 fax 610-898-5871 
 email: tanyaabb...@catholichealth.net 
 
 This electronic mail and any attached documents are intended solely for the 
 named addressee(s) and contain confidential information. If you are not an 
 addressee, or responsible for delivering this email to an addressee, you have 
 received this email in error and are notified that reading, copying, or 
 disclosing this email is prohibited. If you received this email in error, 
 immediately reply to the sender and delete the message completely from your 
 computer system. 
 ___ 
 Histonet mailing list 
 Histonet@lists.utsouthwestern.edu 
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet 
 
 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
  
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Neutrophil staining

[Connolly, Brett M]

Hello Hans,

We had very good staining using Ly-6B.2 from Serotec (MCA771)  on C57B6 mice, 
but needed to switch to Ly-6G for CH3 mice.  The MCA771 datasheet tells you 
which strains react with that antibody.

Brett

Brett M. Connolly, Ph.D.
Principle Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hans B Snyder
Sent: Saturday, September 06, 2014 5:43 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Neutrophil staining

Hello All,

I am looking to  stain neutrophils using special stains and antibodies
in mice tissue.  I wanted your opinion about the best methods to do
so.  I am thinking of using the may Grunwald giemsa and T-blue for
specials and myeloperoxidase and Ly6G for the antibodies.

What are your favorite special stains and antibodies to stain for neutrophils?

Best regards

Hans B Snyder
Histologistics
60 Prescott Street
Worcester, MA 01605
508-308-7800
h...@histologistics.com

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] On the lighter side...

[Connolly, Brett M]

34 years for me, but it seems like just yesterday it was 33.999 years.

Brett M. Connolly, Ph.D.
Principle Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter
Sent: Thursday, August 07, 2014 2:39 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] On the lighter side...

How long have you been a registered histotech?  36 years here.  You???

 

Douglas A. Porter, HT (ASCP) 
Grossing Technician 
IT Coordinator

Cancer Registrar 


CAP-Lab, PLC 
2508 South Cedar Street
Lansing, MI 48910-3138 

517-372-5520 (phone) 
517-372-5540 (fax) 

 mailto:doug.por...@caplab.org doug.por...@caplab.org 

 http://www.caplab.org/ www.caplab.org   

 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Bulk Tissue Procurement

[Connolly, Brett M]

Eric - here a few to check out if you haven't already

http://www.asterand.com/Asterand/

https://www.absbio.com/

http://www.capitalbiosciences.com/

http://www.chtn.nci.nih.gov/

http://ndriresource.org/Human-Tissue-Services/Online-Biospecimen-Catalogue/135/

http://www.tissue-solutions.com/prod_fresh.html

http://www.amsbio.com/home.aspx

Brett

Brett M. Connolly, Ph.D.
Principle Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hanson, Eric
Sent: Wednesday, July 09, 2014 9:51 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Bulk Tissue Procurement

Good morning histonetters!

We are looking for USA vendors for bulk tissue. Mostly we are short on HER2+ 
breast and cervical epidermal tissue (which is punchable, where skin is not). 
We make our own tissue microarray and also use gallbladder, prostate, 
uterus/myometrium, and kidney. Surely we are not the only lab that needs these 
tissue types, and I hope someone out there can point me in the direction of 
some new vendors. 

Thanks,

Eric Hanson
CMI Histotech I

Caris Life Sciences
4610 South 44th Place
Phoenix, AZ 85040
ehan...@carisls.com

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] need recommendations on working alpha synulcein IHC for human tissue

[Connolly, Brett M]

I would also be interested in what Alpha synuclein Abs people are using for IHC
Brett

Brett M. Connolly, Ph.D.
Principle Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maria Mejia
Sent: Tuesday, June 24, 2014 12:15 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] need recommendations on working alpha synulcein IHC for 
human tissue

I'm looking for a working alpha synuclein IHC antibody to be used on human 
tissue.  I work on human
brain stem free-floating 60um sections.  I would greatly appreciate any 
suggestions  recommendations
from anyone.

Regards

Maria Mejia
Histology Supervisor
UCSF
Department of Neurology
SF, CA
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Aperio slide scanner

[Connolly, Brett M]

I will echo Liz's comments. I occasionally scan slides with an older Scanscope 
XT.  It is worth the effort to clean and QC slides for artifacts as Liz points 
out as well as placing the section in the middle of the slide. Actual scanning 
is quite easy, each initial 'snapshot' is reviewed, AOI is resized to 
boundaries of the tissue and focus points are checked and verified to be 
overlaying tissue (not white space).
I find that adding more focus points across the section greatly increased the 
quality of the image and rarely have I found any areas out of focus. Once the 
snapshot reviews are completed simply hit the 'One Touch' icon and batch 
scanning begins.

I really like the ImageScope viewing program which lets one view the section 
and multiple magnifications, capture images, etc.. I have another system for 
IHC quantification, but other users are using the Aperio software to do that if 
that is part of your plan

Brett

Brett M. Connolly, Ph.D.
Principle Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth 
Chlipala
Sent: Wednesday, June 11, 2014 12:46 PM
To: Jan Shivers; histonet
Subject: RE: [Histonet] Aperio slide scanner

Jan

They are not that difficult to operate but you do need person with some 
histology training scanning and QC'ing the slides and images.  The need to 
understand initially if the section is good enough to scan, we QC our slides 
prior to placing them on the scanner, ones that have sectioning artifacts may 
not scan that well.  Section placement on the slide is important, you can't 
have anything to close to the edge of a slide it will not scan well.   The 
person responsible for scanning makes sure that the slide is clean and does not 
have any excess mounting media prior to placing on the scanner, excess mounting 
media and dirt may cause the scan to be out of focus,  and then once the slides 
are scanned the scans need to be QC'd to make sure that they are good enough 
for the pathologist or whatever you are utilizing them for.  Quality and 
consistency in histology preparation is key for good scanning results.  These 
scanners scan in primarily one focal plane, meaning the pathologist loses the 
ability to fine focus as they would on a microscope.  They are able to fine 
focus through poorer quality sections or uneven sections, scanned images do not 
have a fine focus unless you scan them at multiple focal planes or z-stack.  I 
have seen some cytology images that have been scanned in a way that you have a 
fine focus slider but its not common for routine histology preps.

We take some time upfront to adjust area of interest and check focal points 
prior to scanning of the slides.  We find this works better than just going 
with the load and go method.  We review the snapshots prior to scanning.  I'm 
not sure what scanner you are getting or what version of the image capture 
software you will be using we have an Aperio ScanScope XT it has a 120 slide 
capacity.  On occasion a particular slide may not scan well, that’s why we like 
to review the snapshots.  For instance we were working on some amniotic 
membrane constructs these are a single cell layer thick so they are very thin 
and sometimes the computer does not pick the sample up as tissue because it so 
thin and can be lightly stained, so we need to place all of the focal points on 
the slides.  You may not have samples like this but you might.  

Good Luck  - feel free to contact me if you need any help once you get the 
scanner in house.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com

March 10, 2014 is Histotechnology Professionals Day

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jan Shivers
Sent: Wednesday, June 11, 2014 10:25 AM
To: histonet
Subject: [Histonet] Aperio slide scanner

My department may purchase an Aperio slide scanner in the near future.  My 
question is - how tech savvy does one need to be to operate the device?  I have 
staffing concerns and the amount of training time involved.  Thanks in advance.

--
Jan Shivers
IHC/Histology Section Head
Pathology Teaching Program
University of Minnesota
shive...@umn.edu
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), 

[Histonet] dust-free coverslips - do they really exist?

[Connolly, Brett M]

What's your favorite?  It gets frustrating when I need to capture low power 
images and there coverslips are dirty right out of the box.

Fisherfinest Premium isn't bad, but I am wondering if there is something 
better???

Brett

Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803







Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Beta-Amyloid antibody, clone 6E10 availability

[Connolly, Brett M]

Linda, 

We get ours from Covance. 

Brett


Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A
Sent: Thursday, January 02, 2014 4:11 PM
To: Histonet (Histonet@lists.utsouthwestern.edu)
Subject: [Histonet] Beta-Amyloid antibody, clone 6E10 availability

So Histonet land,  our usual vendor for this antibody can no longer get this 
clone.  My question is:  does anyone know who might hold the patent on this 
clone or at least know any company selling the 6E10 clone?

Thanks,

Linda A. Sebree
University of Wisconsin Hospital  Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Questions about IHC in Frozen Sections

[Connolly, Brett M]

I agree with Liz,

We usually fix with acetone/ethanol 5-10 min then go right into buffer, but 
occasionally use 2.0% NBF for some antibodies.  Our buffer contains 0.1% Tween 
and our sections can be anywhere from 8-20um depending on the specific project. 
I think the 30min in acetone is messing up your morphology.

Brett


Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth 
Chlipala
Sent: Thursday, December 05, 2013 5:59 PM
To: Lewis, Patrick; 'Histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: Questions about IHC in Frozen Sections

Patrick

Here is what we do for frozen IHC, this is based upon methods that I received 
from Gayle Callis.

Cut frozen sections and let air dry - at least 20-30 minutes post the last 
section cut.  If we are going to stain that same day or the following day we 
leave the slides at room temp (we are pretty dry here in Colorado) but if you 
have issues with humidity you can store them in a dessicator overnight.

If you need to store at -80 then we package the slides in smaller slide boxes 
and only package enough slides for one run to avoid freeze and thaw artifact.
So once the slides have dried we place them in slide boxes and in those slide 
boxes we add a small or medium nylon tissue bag that contains Silica Gel, 6-16 
mesh (indicating) we just staple the nylon bag shut.  
We then use a food sealer to seal the slide box in one of those food sealing 
bags (we got ours at Cost Co they have them on sale every once and a while, 
along with the bags) and then that goes in the -80 for storage.

The day before we are going to stain we pull out the sealed slide box from the 
-80 and let it sit on the counter top until the next morning when we open up 
and then fix with the best method for the particular IHC that we are going to 
use it could be 10% NBF or 4% paraformaldehyde or one that Gayle recommended to 
us - its an ethanol/acetone mixture - the protocol is listed below.

1.  Fix for 5 minutes in solution made of 75% Acetone and 25% Absolute Ethyl 
Alcohol.

NOTE:  We purchase Absolute Ethyl Alcohol in the small bottles.  Both Acetone 
and Absolute Ethyl Alcohol are both stored in the flammable storage cabinet.

2.  Rinse in two buffer changes for at least 2 minutes.
3.  Continue with staining protocol.

Good Luck

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick
Sent: Thursday, December 05, 2013 3:29 PM
To: 'Histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Questions about IHC in Frozen Sections

Hi Everyone.

I am trying to troubleshoot  my IHC on frozen sections.

My sections are human tonsil at 7 uM. On charged Superfrost slides.

They are stored at -80 after drying for 1 hour.

When I use them for IHC, I take them out of the -80 and let them air dry for 1 
hour before placing them in cold acetone for 30 minutes to fix.

Question:
If I place them directly in H20 or TBST pH 8.0 after fixation, will that cause 
cell lysis?

Should I dry the slides after acetone fixation before washing them?

If so, for how long?

My problem seems to be that the tissue is getting digested on the slide, I am 
trying to trouble shoot which step is causing my tissues to disintegrate.

So far I have tried thicker sections 10, 15 uM (That made the problem worse, I 
am consider going back to 4 uM sections)

I also Changed the concentration of H2O2 for my H202 block from 3% to 0.3%, (In 
my next IHC attempt I will try to examine the slide at each step to see if I 
can see loss of integrity)

Also in my next attempt I plan to eliminate any H20 washes and dry the slide 
post acetone fixation before washing in TBST.

Also I plan to decrease the amount of Tween20 in my Wash buffer from 0.2% to 
0.02%.

Any advice would be helpful.

Patrick.




CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for 
the sole use of the intended recipient(s) and may contain confidential and 
privileged information protected by law. Any unauthorized review, use, 
disclosure or distribution is prohibited. If you are not the intended 
recipient, please contact the sender by reply e-mail and destroy all copies of 
the original message.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] looking for sheep lung blocks

[Connolly, Brett M]

Hi all,

Does anyone know where I could get some FFPE blocks of normal sheep lung?  If 
so, please contact me via e-mail directly.

Thanks,
Brett


Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803








Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] unsubscribe for NSH

[Connolly, Brett M]

Hey - give her SOME credit...she didn't 'uscribe' , 'unscribe' or 'unsuscribe'

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Will Chappell
Sent: Wednesday, September 18, 2013 2:44 PM
Cc: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] unsubscribe for NSH

Oh dear lord. Not Colleen!!  Why man hath wrought??

Sent from my iPhone

On Sep 18, 2013, at 11:42 AM, Colleen Forster cfors...@umn.edu wrote:

 
 
 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Oil red O

[Connolly, Brett M]

I'm not sure, but whatever you do... don't store it next to the Sudan Black 
B...you'll stink up the lab!!

Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of P.E. Visser
Sent: Monday, September 09, 2013 3:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Oil red O

Hi all

I was requested where the O stands for. who has any suggestion.

 

Regards Piet Visser 

Histotech Bronovo The Netherlands

 

 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] old Cell Vision journal article

[Connolly, Brett M]

Hi all,

Does anyone have old Cell Vision journals from 1997?  I am pretty sure it is 
defunct and  I have been trying to get a copy of a paper, can't find what I 
need on the internet. I can provide the specific reference.

Thanks,
Brett


Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803








Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Leica Bond protocol question

[Connolly, Brett M]

Hi All,

I am not a Bond user, but have an antibody were a user had success using 
standard protocol F

Can some please enlighten me with the specifics of that protocol?

Many thanks,
Brett


Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803







Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] HE staining question

[Connolly, Brett M]

Histonetters -

We had some rat tissues that were removed at necropsy and immediately fixed in 
formalin for 48 hrs.  Since the tissues could not be processed after the 48 hr 
fixation they were transferred to 70% ETOH for about a week, then processed and 
stained with HE.

The HE staining looks faded/washed out when compared to previous rat tissue 
that was fixed for 48 hr and then processed and stained without the extended 
time in 70% ETOH. The reason for limiting the fixation to 48 hr in the more 
recent study was that we were doing some IHC stains and wanted to keep the same 
fixation time as the previous study.

I have extra unstained slides left over - any suggestions on how to get some 
better, crisper HE results?  Has anyone observed the washed out HE appearance 
from tissues stored in 70% ETOH.

Thanks,
Brett

Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803






Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: HE staining question - some clarification

[Connolly, Brett M]

I forgot to mention that the HE was performed in another lab at our 
facility...most likely on an autostainer.  We just did the IHC studies and I 
have always used 70% ETOH for tissue storage ... never heard of this staining 
phenomenon happening before.

Brett

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett 
M
Sent: Tuesday, July 02, 2013 9:08 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] HE staining question

Histonetters -

We had some rat tissues that were removed at necropsy and immediately fixed in 
formalin for 48 hrs.  Since the tissues could not be processed after the 48 hr 
fixation they were transferred to 70% ETOH for about a week, then processed and 
stained with HE.


The HE staining looks faded/washed out when compared to previous rat tissue 
that was fixed for 48 hr and then processed and stained without the extended 
time in 70% ETOH. The reason for limiting the fixation to 48 hr in the more 
recent study was that we were doing some IHC stains and wanted to keep the same 
fixation time as the previous study.

I have extra unstained slides left over - any suggestions on how to get some 
better, crisper HE results?  Has anyone observed the washed out HE appearance 
from tissues stored in 70% ETOH.

Thanks,
Brett

Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803






Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Cutting paraffin sections...on a cryostat?

[Connolly, Brett M]

Sure it could be done - low throughput and very awkward though.  So, no other 
lab at the university has a microtome that they would let a fellow researcher 
get some time on ??? 


Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Johnson, Kevin
Sent: Friday, June 14, 2013 4:09 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Cutting paraffin sections...on a cryostat?

Hi, all.  A bit of an odd question: a colleague knows of someone wanting to cut 
paraffin sections who has a cryostat, but no microtome. Since a cryostat's 
basically a microtome in a freezer chamber, I thought that it may be awkward, 
but theoretically doable once it was brought to room temp and dried out 
thoroughly. However, I wondered if lubricants formulated for the cold might 
become too thin for use at room temp, possibly causing damage to moving parts.  
Any thoughts?

Kevin Johnson
University of Miami
Diabetes Research Institute
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] antibodies online

[Connolly, Brett M]

I find that the individual antibody search engines I use each provide info from 
select companies so I generally look at several ...in addition to Biocompare, 
Google, Human Protein Atlas and PubMed searches.

http://www.antibodyresource.com/home

http://www.antibodydirectory.com/index2.php

http://www.labome.com/

http://www.linscottsdirectory.com/

http://www.nordiqc.org/Default.htm

and, here is one for working on murine tissue specifically, I don't think it 
gets updated though  

http://ncifrederick.cancer.gov/rtp/lasp/phl/immuno/

Brett

Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803












-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours
Sent: Tuesday, May 28, 2013 9:39 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] antibodies online

Hello Histonetters!

What search engines do you use to find antibodies that are available
commercially? I use biocompare, but I'm not sure if that's the best site.
 I would just google it, but that brings up wy too many protocols and
papers (though the papers are sometimes a good source as well)
Searching individual companies sometimes helps, but I've found that
anti-chick antibodies are usually sold by small companies.

Emily


By bitching and bitching and bitching, they could exhaust the drama of
their own horror stories. Grow bored. Only then could they accept a new
story for their lives. Move forward.

-Chuck Palahniuk, Haunted
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Adenosine 2A receptor IHC

[Connolly, Brett M]

Hi All,

Looking for antibodies for the A2A receptor for FFPE tissues - any suggestions 
would be appreciated.

Thanks,
Brett

Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.commailto:brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] HIER pressure cookers....

[Connolly, Brett M]

Biocare Decloaker here !!

Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of k. leigh adams
Sent: Tuesday, April 30, 2013 9:30 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] HIER pressure cookers

Any input as to preferred instruments would be greatly appreciated...

Leigh
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Whole slide scanners.

[Connolly, Brett M]

I think Scott makes a good point about having a clear need.  We use the Vectra 
system now, and have previously used Ariol. We only scan IHC slides from 
projects in which we want a quantitative analysis.

Brett


Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott A. Ely
Sent: Thursday, April 11, 2013 2:05 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Whole slide scanners.

I have worked with whole slide scanners and digital image analysis since 1998.  
We currently own scanners from 3 different vendors.  Also, I have participated 
in whole slide scanner marketing research, sitting at a discussion table with a 
dozen people from different hospitals.

My experience has been that most departments purchase scanners without a clear 
need.  For example, some want to scan and archive slides, to make them 
available for review.  That makes some sense in theory.  However, in every 
institution I've talked to, they end up paying for the scanner, a tech to do 
the work, the service contract and the server space to store the images, which 
can be huge, up to 20 *giga*bytes per *slide... then find that it is rare for 
anyone to ever actually look at the scanned images.  Most pathologists find it 
is more expedient to simply pull the glass slides out of the archive.  In New 
York, we are required by law to archive glass slides for at least 10 years 
anyway.

So, my advice, for what it's worth, is not to purchase any whole slide scanner 
unless you have a clear and well defined need (we have 3 giant systems, rarely 
used, taking up space and gathering dust).  For example, if you need to store 
images from consultation slides before returning them to another institution, 
why not just snap a few photomicrographs?  Being able to view a couple of 
small, representative pictures is better than having to wade through an entire 
scanned slide.

---
Scott Ely, MD MPH
Associate Director, Hematopathology Fellowship Program
Section of Hematopathology
Department of Pathology
Weill Medical College of Cornell University
New York Presbyterian Hospital
Room:  Starr 715
525 E. 68th Street
New York, NY 10065
PH:  212-746-2442
FAX: 212-746-2009
http://www.cornellphysicians.com/scottely/

Legal Confidentiality Notice: This e-mail message, including any attachments, 
is for the sole use of the original intended recipient(s) selected by Dr. Ely 
and may contain confidential and privileged information.  Any unauthorized 
review, use, disclosure or distribution is prohibited. If you are not the 
recipient specified by  Dr. Ely, please contact the sender by reply e-mail and 
destroy all copies of the original message.
---


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of 
histonet-requ...@lists.utsouthwestern.edu 
[histonet-requ...@lists.utsouthwestern.edu]
Sent: Thursday, April 11, 2013 1:17 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 113, Issue 10

Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-requ...@lists.utsouthwestern.edu

You can reach the person managing the list at
histonet-ow...@lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific
than Re: Contents of Histonet digest...


Today's Topics:

   1. Whole slide scanners, any reviews..? (Erickson, Jamie E)
   2. RE: Whole slide scanners, any reviews..? (Elizabeth Chlipala)
   3. RE: Whole slide scanners, any reviews..? (Elizabeth Chlipala)
   4. Want better IHC / ISH results with 10+ or even 20+ year   old
  FFPE specimens? (Rob Day)
   5. Micro-cut H1200 Vibrotome manual (Jo-Ann Bader, Ms.)
   6. Talk to me about shelf life and outdates (Cheryl)
   7. RE: Talk to me about shelf life and outdates (Elizabeth Chlipala)
   8. FFPE for Immunohistochemistry. (Ian R Bernard)
   9. Formalin Neutralizer (Bustamante, Lin)
  10. compression problems (Denise G Crowley)
  11. Re: Formalin Neutralizer (Cristi Rigazio)
  12. Re: Talk to me about shelf life and outdates (Emily Sours)
  13. RE: compression problems (Elizabeth Chlipala)
  14. RE: Talk to me about shelf life and outdates (Goins, Tresa)
  15. Re: FFPE for Immunohistochemistry. (Geoff)
  16. RE: FFPE for Immunohistochemistry. (Morken, Timothy)
  17. Anti-human nuclear antibody (Elizabeth Cameron)
  18. 

[Histonet] Processing question

[Connolly, Brett M]

Histonetters,

Has anyone tried to process an intact mouse brain to paraffin?


Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803








Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] What's your favorite mouse-on mouse detection kit

[Connolly, Brett M]

Hi All,

For those of you working in research on animals tissue I'm looking for a 
consensus of opinions on kits for HRP mouse- on -mouse detection.

Would like to avoid biotinylation procedures (i.e.ARK).

Thanks,
Brett


Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803






Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] CD31 problewms

[Connolly, Brett M]

I think the CD31 Ab from Dianova is what works best for most people on FFPE 
mouse tissue. 

Brett

Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Thursday, February 14, 2013 12:27 PM
To: Jo-Ann Bader, Ms.; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] CD31 problewms

If you have not changed the Ab provider nor any step on your protocol, check 
the Ig concentration in the lot you are using now compared with the lot you 
used to determine the Ab dilution. If the Ig is less concentrated now, you may 
need to increase the concentration (reduce the dilution).
René J.
 

From: Jo-Ann Bader, Ms. jo-ann.ba...@mcgill.ca
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu 
Sent: Thursday, February 14, 2013 11:49 AM
Subject: [Histonet] CD31 problewms

We are having problems getting our CD31 to work well on our NBF fixed mouse 
tissue. There is staining but it is very pale.

The CD31 comes from Bio-care and we are are using the IntelliPATH stainer.

We have tried:

- Trypsine 37C 15 min / Trypsine RT 30 min
- Trypsine (1:2 and 1:3)
- With Decloaker / without decloaker
- DAB Sparkle / No DAB sparkle
- Different dilution of CD31 (1:50 / 1:25)
- Different time of CD31 incubation (2h / 3h)
- Different time of incubation of secondary and tertiary reagent (10min to 
20min)

Thanks


Jo-Ann Bader
Histology Co-Ordinator
Goodman Cancer Centre
McGill University 1160 Pine Ave. W - Rm 312
Montreal, QC, Canada
H3G 1Y6
Tel: 514-398-8270
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] CD45 for FFPE mouse tissue

[Connolly, Brett M]

Hi all,
What's your favorite anti-CD45 for mouse... Serotec rat monoclonal or something 
else?

Thanks,
Brett

Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803






Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] cd11b and cd11c for FFPE mouse tissue

[Connolly, Brett M]

Any favorites for CD11b and CD11c for little mice FFPE tissue ? Has anyone used 
the anti-CD11c antibodies grown in Armenian hamster?

Thanks,
Brett


Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803






Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] tricks about antibody generated in human on human tissue for IHC

[Connolly, Brett M]

Hi Carole,

While I have no direct experience with human on human IHC, my first thought 
would be to biotinylate your primary antibody and then you would omit the 
anti-human secondary which is the most likely source of your background 
staining. 

If you are using fluorescence you could also conjugate your primary with a 
fluorophore  
(http://www.invitrogen.com/site/us/en/home/brands/Molecular-Probes/Key-Molecular-Probes-Products/Zenon-Labeling-Technology.html)



Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
carole.chot...@fr.netgrs.com
Sent: Friday, January 11, 2013 5:32 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] tricks about antibody generated in human on human tissue 
for IHC

Dear all,

 

I work in an histopathology department and we intend to do
immunohistochemistry with an antibody generated in human on human
tissues. So far, we don't have any experience with that. So I was
wondering if somebody would have some feedback to prevent strong
background generated by this human antibody on human tissue. We are
planning in a first place to decrease the concentration of the secondary
antibody Donkey@human which is recommended at 1/200 by Jackson, using it
at 1/500 for example.

 

But if anybody would have some experience with this kind of experiments,
their tricks would be more than welcome.

 

Best Regards,

 

Carole Chotard

 

 

Carole Chotard, Ph.D.

Pharmacology Research Scientist

Molecular Pharmacology and Pathophysiology Department

IDRS

125 Chemin de Ronde

78290 Croissy-sur-Seine

France

Phone : +33 1 55 72 29 48

Fax :   +33 1 55 72 25 96

carole.chot...@fr.netgrs.com

 

 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Control Tissue

[Connolly, Brett M]

Amy -  here are a few...

Cooperative Human Tissue Network- http://www.chtn.nci.nih.gov/

Asterand biorepository. -  https://www.asterand.com/Asterand/

NDRI - http://ndriresource.org/

Analytical Biological Services, Inc. -  
http://www.absbioreagents.com/products/products.html


Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803












-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Johnston, Amy
Sent: Tuesday, January 08, 2013 7:07 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Control Tissue

Is there any way to obtain control blocks? We are in the process of starting up 
a new histology lab and control slides are a lot more expensive than I 
thought:( .  I have purchased several boxes already, I was hoping I could find 
the others as they should be easy to find.  (I tried NSH, they no longer have a 
bank of blocks.)  I still need controls for trichrome, iron and mucin, any 
suggestions?

Amy Johnston HT(ASCP)
Histology Technician
Oregon Medical Group
4140 Quest Drive
Eugene, OR 97402
541-463-2163

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: VIP6 issue

[Connolly, Brett M]

One of the products I learned about at the 2012 NSH convention was ActivFlo 
tissue cassettes. Designed by JB McCormick (I think) they are biopsy cassettes 
that have side vents to help disperse the trapped air bubbles.

Here is a link from Leica, they sell several different ActiveFlo cassettes... 

http://www.leicabiosystems.com/products/consumables/cassettes-base-molds/biopsy-cassettes/
 

Brett


Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich
Sent: Thursday, December 13, 2012 12:45 PM
To: Morken, Timothy; Nancy Schmitt; Histonet (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] RE: VIP6 issue

Tim brings up a good point.
I would monitor the processing in the fine mesh type cassettes as well. For 
years, and I do mean years, we struggled with our tiny specimens. Why did some 
specimens in mesh cassettes do just fine, some notit was an absolute 
nightmare! Monitored how the specimen was handled starting right at the sight 
of surgery on through changing processing schedules/times/heat?/no 
heat/xylene/xylene substitute/staining schedules. We wet the mesh cassettes 
prior to loading, then trying to swish the air pocket out of the cassette 
before processing. (did not experience the lids coming off)

We found that the only thing that has produced consistently good results was 
when we went back to the lens paper. What we think was going on, was that the 
little biopsies would intermittently get caught up in a air bubble during 
processing (agitation, pumping in/out), thus missing being in that current 
solution(s). It truly was a nightmare.. 

We now purchase cheap lens paper, and cut it into 4 squares..paper cutter 
does a large amount quickly and we're good to go. Yes, it is a little more 
hassle unwrapping them in the morning, but if it gives a better quality 
specimen then it doesn't matter!! 

Just my two cents worth;)
Lynette

Lynette Pavelich, HT(ASCP)
Histology Supervisor
Hurley Medical Center
One Hurley Plaza
Flint, MI 48503

ph: 810.262.9948
mobile: 810.444.7966


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Morken, Timothy 
[timothy.mor...@ucsfmedctr.org]
Sent: Thursday, December 13, 2012 12:03 PM
To: Nancy Schmitt; Histonet (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] RE: VIP6 issue

 I talked with the rep. and they said they had never heard of this.

Right.

Yes, it happens. Often. Too much air caught in the cassettes. Worse with the 
fine-mesh cassettes. Bounce the racks in the formalin tray a few times before 
loading on the processor to try to get as much air out as possible. That will 
improve processing as well. And weight down the tops if it continues.


Tim Morken
Department of Pathology
UC San Francisco Medical Center




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt
Sent: Thursday, December 13, 2012 6:17 AM
To: Histonet (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] VIP6 issue

Good Morning-
We have recently purchased  VIP6 processors.  Has anyone else experienced a 
problem with the lids coming off during pump in and pump out?  Causes the 
cassettes to float all over and be completely out of order:(  We now place an 
extra rack or lid on top to weigh down and insure this does not happen.  I 
talked with the rep. and they said they had never heard of this.  I know this 
is not  a huge deal, but with new instrumentation I don't think we be cobbling 
things already.
Thank you for any input-
Nancy Schmitt
Dubuque, IA



NOTICE: This email may contain legally privileged information. The information 
is for the use of only the intended recipient(s) even if addressed incorrectly. 
If you are not the intended recipient, please notify the sender that you have 
received it in error and then delete it along with any attachments. Thank you.



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 

[Histonet] RE: formalin substitutes. HELP

[Connolly, Brett M]

Hello Nieves,

Here in the US Anatech LTD sells a gyloxal fixative called Prefer. I have 
attached a link to the website. Go to the MSDS menu and click on 'Prefer' to 
pull up the MSDS. 

http://www.anatechltdusa.com/

Below is an old  post from the HistoNet that might be helpful. I suggest you 
contact Ada Feldman at Anatech, as she should be able to address your issues

Regards,
Brett


Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803



As the developers of the Prefer fixative we would like to address  
some of the issues.

Interchangeability of glyoxal products: The manufacturer of each  
fixative should be able to provide the information necessary to work  
with their fixative in conjunction with any other fixative that a lab  
may be using. Just as an example, Hollandes is not compatible with  
NBF and requires special attention. As for Prefer we can say it is  
compatible with the majority of other fixatives, glyoxal or not.

Limited time in the fixative: There is a slight reduction in staining  
intensity after several weeks, but increasing staining time corrects  
this. So tissues are not rendered unstainable.

Transition period: This is true any time you are switching fixatives  
or processing methods.

Eosinophila: Your statements here are true.

Lysis of erythrocytes: True again. It is often seen as an advantage  
because diagnostics cells are easier to see.

Breast cancer: It would seem that the improved nuclear morphology  
would make marked nuclear variation easier to determine.

Prostate biopsies: Hope you can get a response from any of the  
glyoxal users with prostate biopsies. We would be interested  in this  
answer also.


Ada T. Feldman, MS, HT/HTL(ASCP)
ANATECH LTD.
1020 Harts Lake Road
Battle Creek, MI 49015

Phone: 800.262.8324
Fax: 269.964.8084
email: adafeld...@anatechltdusa.com
website: www.anatechltdusa.com


On Jul 14, 2006, at 4:13 PM, rsrichm...@aol.com wrote:

 The people at Anatech, makers of Prefer fixative, have published a  
 review of
 glyoxal fixation that every pathologist and histotechnologist ought  
 to read.
 This working surgical pathologist would like to add - and solicit -  
 some
 comments on Histonet.

 Glyoxal Fixation and Its Relationship to Immunohistochemistry.  
 Richard W.
 Dapson, Ada T. Feldman, and Dee Wolfe. Anatech Limited, Battle  
 Creek MI. The
 Journal of Histotechnology June 2006;29:65-76.

 I don't want to change, but I think we all need to be prepared for  
 the day
 when a manager walks into our laboratory, or a letter from a  
 regulatory agency
 arrives in the mail, telling us that we have to get rid of  
 formaldehyde right
 now. Probably glyoxal is the only acceptable substitute, and we all  
 need to
 have a look at it. I have a number of questions.

 Interchangeability of glyoxal products: Prefer is described as a  
 buffered
 solution of glyoxal with a pH of about 4. The formula is a trade  
 secret.
 Competing glyoxal products probably also have trade-secret  
 formulas. So can a lab
 change brands of buffered glyoxal without problems, or does it have  
 to stay with a
 particular brand with its trade-secret buffering? - We've seen a  
 similar
 problem with distilling aliphatic xylene substitutes: every one of  
 them requires a
 separate distillation routine, at least on a spinning-band still. -  
 I'll
 leave it to John Kiernan to comment on the appropriateness of trade- 
 secret
 reagents in histopathology.

 Limited time in the fixative: Tissue can be left in neutral  
 buffered formalin
 for quite a long time and still be stainable, but tissue stored in  
 glyoxal
 becomes unstainable after about two weeks. Can glyoxal fixed tissue be
 transferred to 70% ethanol for more prolonged storage? - A very  
 occasional surgical
 specimen requires additional blocks after a week - a bigger problem  
 will be the
 pathologist who doesn't trim his autopsies promptly.

 Transition period: A laboratory changing to glyoxal would have to  
 keep IHC
 procedures for both fixatives working for some time. There would  
 have to be some
 way to identify whether a block was fixed in formaldehyde or glyoxal.

 Eosinophilia: One ought to be able to distinguish eosinophils from
 neutrophils in tissue sections by nuclear morphology, without  
 having to see granules.
 But quantitation of eosinophils - needed in an increasing number of  
 GI biopsy
 situations - could be a problem. We might need an IHC for  
 eosinophils in some of
 these settings.

 Lysis of erythrocytes: Not much of a problem, since we're used to  
 it with
 acid fixatives anyway.

 Breast cancer: Elimination of nuclear bubble artifact in breast biopsy
 specimens may raise the apparent nuclear grade of tumors, and thus  
 increase
 Nottingham (Elston-Ellis) scores.

 Prostate biopsies: I'd want to see some prostate biopsies - is  
 somebody from
 OURLab in 

RE: [Histonet] Microtome knives

[Connolly, Brett M]

Hi Jon,

Depends on your cash flow. You could get a used sharpener somewhere off the web 
somewhere such as 
http://www.labx.com/v2/adsearch/resultsnew.cfm?sw=sharpenermr=25te=cat , or 
http://www.medwow.com/used-microtome-knife-sharpener-equipment/63.med but 
sharpening knives is a pain IMO and steel knives present more of a safety 
hazard. I would recommend a sharpener that uses the glass honing plates. You 
would also need the coarse and fine abrasives.

Personally, I would opt for a low profile disposable blade holder that fits 
your 820. Low and high profile refer to the size (height) of the blade. We use 
low profile for paraffin block sectioning and high profile for cryostat 
sectioning.

Brett

Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jon Krupp
Sent: Friday, November 09, 2012 1:50 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microtome knives

Greetings

I need some advice regarding microtome knives. I am not  histo tech, I did all 
my sectioning in a plant research lab, but now I find myself needing to learn 
more about histo type methods.

We have microtomes, AO 820's, and we have a bunch of donated knives. I need 
advice about whether it would be better to find a knife sharpener and use the 
microtome knives we have, or check into getting a disposable knife holder. 

When I was sectioning, we just used a simple razor blade holder. Now I see 
references to high profile and low profile blades and holders, and I don't know 
the difference. 

Anyone willing to help me out?

Thanks

Jon

Jonathan Krupp
Delta College
5151 Pacific Ave.
Box 212
Stockton, CA  95207
209-954-5284
jkr...@deltacollege.edu

Find us on Facebook @
Electron Microscopy at SJ Delta College







___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] How to fix frozen section?

[Connolly, Brett M]

Amy,

We use ice-cold acetone/ethanol (3:1) for 10'...works for all of our antigens 
so far.  


Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Lee
Sent: Tuesday, November 06, 2012 2:41 PM
To: histonet
Subject: [Histonet] How to fix frozen section?

Hello,
 
I will receive some frozen tissue sections that are not fixed. I rarely do IHC 
on frozen sections. I searched some articles about fixation. Some use 50/50 of 
acetone/alcohol and some use only alcohol. Could you please recommend a 
fixation method for me?
 
 
Thanks,
 
Amy
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Dako vs Ventana IHC systems

[Connolly, Brett M]

Ditto Linda's comments. We've had an Intellipath for several years. 50 slide 
capacity, flexible open system- multiple primaries in one run, use any reagents 
you want, good tech support.

Even Hurricane Sandy can't blow it away...

Brett Connolly, PhD
Imaging Dept.
Merck Research Labortories
West Point, PA 19486


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda 
[lbla...@digestivespecialists.com]
Sent: Monday, October 29, 2012 4:00 PM
To: 'Joe W. Walker, Jr.'; CHRISTIE GOWAN; terri.br...@northside.com; 
lseb...@uwhealth.org; lynn.bur...@illinois.gov; gth...@pcasoutheast.com; 
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Dako vs Ventana IHC systems

Joe,

For a smaller lab the Intellipath from BioCare works great.  Since it's has the 
capabilities to do continuous load or a STAT run the workflow works great.  
I've had mine for three years now and the service and support have been the 
best I've ever encountered.  It truly is an open system too.
If you want any more info feel free to contact me directly.
Linda

Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe W. Walker, 
Jr.
Sent: Monday, October 29, 2012 3:55 PM
To: CHRISTIE GOWAN; terri.br...@northside.com; lseb...@uwhealth.org; 
lynn.bur...@illinois.gov; gth...@pcasoutheast.com; 
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Dako vs Ventana IHC systems

Thank you for this information, Christie.  We are a much smaller lab and would 
be using the new system as our primary IHC stainer.  Workflow considerations 
are a primary concern for us in addition to staining consistency and ease of 
use.  From what was demonstrated to us, the Ultra did not appear to be that 
closed and provided lots of flexibility for running just about anything we 
wanted but it was a sales pitch.  We are still in the researching our options 
stage.

We have experienced less than desirable service from Dako but they currently 
have a presence in our lab.  We have been pretty happy with the antibodies from 
Dako.

Joe W. Walker, Jr. MS, SCT(ASCP)CM
Anatomical Pathology Manager
Rutland Regional Medical Center
160 Allen Street, Rutland, VT 05701
P: 802.747.1790  F: 802.747.6525
NEW EMAIL: joewal...@rrmc.orgmailto:joewal...@rrmc.org
www.rrmc.orghttp://www.rrmc.org

Our Vision:
To be the Best Community Healthcare System in New England

Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet 
Recognition(r) and the Governor's Award for Performance Excellence

From: CHRISTIE GOWAN [mailto:christiego...@msn.com]
Sent: Monday, October 29, 2012 12:15 PM
To: Joe W. Walker, Jr.; terri.br...@northside.com; lseb...@uwhealth.org; 
lynn.bur...@illinois.gov; gth...@pcasoutheast.com; 
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Dako vs Ventana IHC systems

Hi Joe,
When searching for an automated IHC stainer, you need to shop based on your 
facility and it's needs. We recently went through the whole shopping around 
experience and because we are such a large facility, we have the Ventana Ultra 
as well as an XT and the New Dako Link. We use the Ventana Ultra for single 
piece flow and it really changed the dynamic of our lab. We also use the 
Ventana for all our automated probes including the dual Her2/Chr17 CISH. The 
Dako's (we have 2) we use for all those things that we do not run on the Ultra 
or Benchmark XT. About 50/50 on each system. We also do a lot of research for 
the UAB campus and it is nice to have the more open DAKO systems. We have 3 
very experienced IHC techs working in the lab which lends a lot of consistency 
to the staining. I say this because if you rotate people through the lab or 
your staff is less experienced then you want something that is easy and 
consistent. We will probably always have the Ventana systems in our lab so not 
much shopping around for a more or less closed system (there is always debate 
over the open/closed thing) However, shopping around for a more open system can 
be a little harder. We demo'ed the bond, Biocare, Dako and Labvision. We 
narrowed it down to Biocare and Dako based on ease of use, speed and capacity 
and reagents/antibodies. Once we had narrowed it down to the 2 companies, then 
we started looking at packages. Dako delivered the best package and because 
Dako already had a presence in our Histology lab (Artisan Special Stainer) and 
we knew their service was always prompt (we always struggled with this with 
Leica) We chose Dako and replaced our 2 older Labvisions with Autostainer Links.
If you have any questions you can let me know via email and I will give you our 
contact info. Best of luck to you.
Christie Gowan
UAB Hospital

[Histonet] RE: Cold room alternative

[Connolly, Brett M]

Ditto here, ours are manufactured by True, but you could probably find one 
cheaper through one of the used lab equipment vendors on the web.

Brett

Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bell, Pat
Sent: Tuesday, October 02, 2012 6:13 PM
To: 'Johnson, Aaron'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Cold room alternative

Aaron,

We have a refrigerator from VWR that has a glass door like a Deli frig. and 
it has an electrical outlet inside as well as outside. That could be a starting 
place to look. :)

Pat Bell HT(ASCP)
University of Colorado, Denver
Medical Oncology; MS 8117
12801 E 17th Ave.
RC1-S, L18-8402C
Aurora, Co. 80045
303-724-6077
pat.b...@ucdenver.edu
 
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Johnson, Aaron
Sent: Tuesday, October 02, 2012 3:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cold room alternative

The building I am in does not have a cold room and I would like to incubate 
some samples overnight on an orbital shaker at 4°C. Can anyone recommend a 
refrigerator with an interior electrical outlet or another alternative? Thanks.
Aaron

Aaron M. Johnson
Assistant Professor
Department of Speech and Hearing Science University of Illinois at 
Urbana-Champaign

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Cooling paraffin blocks with ice VS. Freezing Spray

[Connolly, Brett M]

I agree with Rene and Tom. You have learned the skills and need to be in a 
place where you are happy and in an environment that fosters development, 
cooperation, and innovation.

What you have described is the opposite situation. Hopefully you can find a 
better home in your area, not a my way or the highway environment.

Those that resist change, or an open exchange of ideas, eventually fall by the 
wayside... so good luck with your search.

Brett

Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jenny Vega
Sent: Monday, October 01, 2012 5:02 PM
To: Rene J Buesa; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Cooling paraffin blocks with ice VS. Freezing Spray

I haven't mentioned that the hospital where I work has been facing
financial problems and my job has been threatened a couple of times. My
work hours have been reduced from 40 hours a week to 32 since Feb and seems
that they have no intentions of increasing them again plus I am underpaid
with no benefits which contributes to the problem. This is already a good
reason to change jobs.

I don't want to personally attack people who haven't gotten a degree in a
specialized histotech school because I think that everybody has unique
abilities to offer.

In my case I consider myself a theoretical person because is easy for me to
grasp concepts that is why I had success graduating from histotech school
and passing my certifications but I have struggled a bit in the technical
aspect which is very important but I have learned a lot of different
techniques that have benefit me and I never stop learning. I only have 1
year and 6 months of experience in my current job cutting, staining and
doing frozen sections and I have been getting better.

There are other people on the other hand who have never been in histotech
school or don't have a strong theoretical foundation but they have good
technical skills like my supervisor for example. My supervisor seems that
she has never sat down to study a book about histotechnology and is not
that important to her.That is why I have asked here certain questions
before that to some people are silly . I sometimes can't believe that she
doesn't know those things and I get confused as well and wonder if I am the
one who is wrong.

Like the time when I asked if 10% formalin equals 4 to 3.7 formaldehyde
because my supervisor said that they were different things and she was
sending back all the 10% formalin bottles to the provider because she has
used formaldehyde all the time. The manufacturer contacted her and educated
her on the subject but she still thinks that they are not the same thing.
She recently said that formaldehyde is more concentrated and toxic than 10%
formalin. I don't correct her because she is going to get offended.

And the other question I asked about xylene and protective gloves. She
discouraged the use of gloves because they latex would mark the back of
the slides while cover slipping. I suggested the idea of buying nitrile
gloves but her response was There is no money for that so I had to buy my
own nitrile gloves.

Someone here once sent me a MSDS sheet with all the possible side effects
that xylene can cause and I read that it can contribute to thyroid disease
and my supervisor currently has issues with her thyroid so who knows if
this has contributed to that problem for not using gloves for many years.


My supervisor is a very stubborn person and she likes to be right all the
time so I don't say anything every time she says something incorrect so
dealing with her is not that easy.

Since I only have been for a year I really want to get more experience
before changing jobs. If I don't see no improvement in the economic
situation that the hospital is facing then I will have to leave.

Thanks for your advice

On Sun, Sep 30, 2012 at 10:17 AM, Rene J Buesa rjbu...@yahoo.com wrote:

 Jenny:
 There is a saying that life is too short to drink cheap wine. In the
 same way life is too short to be frustrated daily working at a place where
 work is like a daily uphill battle.
 For what you describe you know your trade. Start looking for another place
 although do not expect that your ideas will always be well received.
 Older, trained on the job, and with lots of experiences supervisors
 usually are not very open to suggestions, especially when those ideas
 conflict with what they are used to do because they do not know the
 scientific basis of what they are doing.
 The less open to suggestions a person is, the more ignorant they are
 likely to be.
 René J.

   *From:* Jenny Vega histotech...@gmail.com
 *To:* joelle weaver joellewea...@hotmail.com;
 histonet@lists.utsouthwestern.edu
 *Sent:* Saturday, September 29, 2012 6:26 PM
 *Subject:* 

RE: [Histonet] How can i post a question in histonet?

[Connolly, Brett M]

You just did.

Brett

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of arjun ruhella
Sent: Thursday, September 20, 2012 1:49 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] How can i post a question in histonet?

Please let me know!

Thank you
Best,
AR
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Formalin and Operating Rooms

[Connolly, Brett M]

Here is a notorious case from my days working in a Ft. Lauderdale suburban 
hospital. Along with Bill's experiences we took formalin out of the OR after 
this was reported, but it wasn't a requirement as far as I remember.

http://www.nytimes.com/1985/03/10/us/one-death-many-questions-in-miami.html?pagewanted=all

Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803


 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Tuesday, August 21, 2012 9:05 AM
To: Paula Sicurello; Debra Siena
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Formalin and Operating Rooms

I know of no rules, though there may well be. I do know that several
years ago a patient was injected (IV)with formalin in the OR. Results
were NOT good. I know that the OR in the place I was working at the time
would no longer allow for formalin in the OR suite. The tissues were put
in formalin in a separate area - by OR staff.  

Bill

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula
Sicurello
Sent: Monday, August 20, 2012 6:05 PM
To: Debra Siena
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Formalin and Operating Rooms

I would be interested in the replies as well.  To add to that, I've
heard the same thing about glutartaldehyde, do the same rules apply?

Thanks,
Paula
--
Paula Sicurello, HTL (ASCP)
Supervisor, Clinical Electron Microscopy Laboratory Duke University
Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina
27710
P: 919.684.2091

On Mon, Aug 20, 2012 at 6:25 PM, Debra Siena dsi...@statlab.com wrote:

 Hi All,

 I would like to ask if anyone has heard of any new regulations or laws

 that state that the Operating Room can't have formalin available in 
 the room so that they can place formalin onto the sample right away?  
 I was wondering if anyone has heard of this, if you could tell me more

 about where it is coming from so that I can access a copy of it.  We 
 have had some inquiries and I have not heard of this.  I appreciate 
 any help that you can give and sorry, that I don't have more
information.  Best wishes.

   Debbie Siena, HT(ASCP)QIHC
 StatLab Medical Products
 Technical Support Manager
 407 Interchange Street | McKinney, TX 75071
 t: 800.442.3573 ext. 229 | f: 972.436.1369 dsi...@statlab.com | 
 www.statlab.com


 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This electronic mail and any attached documents are intended solely for the 
named addressee(s) and contain confidential information. If you are not an 
addressee, or responsible for delivering this email to an addressee, you have 
received this email in error and are notified that reading, copying, or 
disclosing this email is prohibited. If you received this email in error, 
immediately reply to the sender and delete the message completely from your 
computer system.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] (no subject)

[Connolly, Brett M]

 Second that - learned that trick for cleaning the water bath surface from Pete 
Emanuele at AFIP a looong time ago.

Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda
Sent: Tuesday, July 31, 2012 3:05 PM
To: 'Paula Pierce'; Histonet
Subject: RE: [Histonet] (no subject)

Old telephone book pages!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce
Sent: Tuesday, July 31, 2012 2:23 PM
To: Histonet
Subject: [Histonet] (no subject)

Greetings,

does anyone know of a less expensive substitute for Kim-wipes?

 
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
8901 S. Santa Fe, Suite G
Oklahoma City, OK 73139
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] any news on CD4, CD8 IHC on FFPE mouse tissue?

[Connolly, Brett M]

I'm wondering if anyone has recommendations for CD4 and CD8 antibodies for FFPE 
mouse tissue. Looking through the archives I see there was only success on 
frozen tissues.

Has anything changed recently?? Or should I stick with CD3.

Thanks,
Brett

Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803







Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Black Biopsy Paper

[Connolly, Brett M]

Becky,  

We have used this paper. What I found is that when submersed in the formalin 
the black dye leaches out of the paper and the portion of the tissue touching 
the paper turned somewhat black. These were tissues left on the paper and in 
formalin overnight. Since I never processed that tissue I don't know if the 
black washes out during processing.

Brett

Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rebecca LeVier
Sent: Wednesday, July 18, 2012 2:26 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Black Biopsy Paper

I am wondering if anyone out there is using black biopsy paper to give to 
offices to place the biopsy on prior to putting it into formalin? It looks kind 
of like construction paper. If you are using this can you please give me a list 
of pros and cons associated with it?

Thanks,
Becky


Disclaimer Note: The information in this message with any attachment to it, may 
be privileged and protected from disclosure. If the reader of this message is 
neither the intended recipient, nor an employee or agent responsible for 
delivering this message to the intended recipient, then you are hereby notified 
that any dissemination, distribution, unauthorized use, or copying of this 
communication is strictly prohibited. If you have received this communication 
in error, please notify us immediately by replying to this message and deleting 
it from your computer. Thank you.


...

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] RE: Re: Methanol in H2O2 explanation

[Connolly, Brett M]

Now that's funny! Even if it makes we PhDs look bad. 

I was once given some tissue in formaldehyde from one of my colleagues in 
another department to process and stain. The resulting slides looked horrendous 
and I suspected the fix was the problem. I went back to their lab and asked to 
see the bottle of formaldehyde which turned out to be formamide. Then I got 
the shoulder shrug and We thought it was close enough response. Argghh.

Brett

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Geoff McAuliffe
Sent: Wednesday, July 11, 2012 12:50 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: Re: Methanol in H2O2 explanation

I agree, lots of lore gets passed down without question or understanding.

Many years ago a new PhD who had trained in a good lab came to my lab 
looking for some NaH. I tried to explain that there was no such 
chemical when she produced a xeroxed lab protocol calling for NaH to 
make a malete buffer for EM (that shows you how long ago this was!). So 
I found the exact recipe but with NaOH. The techs all knew it was a typo 
but she did not.

Geoff

On 7/11/2012 12:12 PM, Morken, Timothy wrote:
 It's funny how histology lore gets passed down, passed around and 
 misunderstood over the decades. When I ask questions in labs about why they 
 do a certain procedure they can rarely name a source (besides a particular 
 long-retired particular tech who wrote the original procedure) or give reason 
 as to why it is done that way. Even if it works well, at least people could 
 take the time to understand why!

 When I was researching buffer compositions long ago I found a dozen 
 variations of PBS alone (and only a dozen because I just stopped looking). 
 When you delve into these things it becomes clear that people made their own 
 solutions for a particular purpose. Maybe they wrote a paper, taught a course 
 or wrote a book. Then that formula was copied by many others and their 
 particular formulation became the state of the art even if the art 
 practiced by someone else had little to do with the original pursuit (the 
 Sheehan book is full of variations of stains that were originally for 
 specific research purposes. There is precious little guidance in any books 
 about what is good for a particular use!).

 The reason for using methanol / H2O2  that I was told long ago was prevent 
 frozen sections from being damaged by H2O2 bubbling. In fact, we would fix 
 frozens in cold acetone and THEN put the slides in cold methanol/H2O2 (double 
 fixation?)  In that lab we never used methanol for deparaffinized sections. 
 Since I had no exposure to the clin lab I never knew about using methanol for 
 blood smear fixation. From literature searches it seems methanol, rather than 
 ethanol, is used primarily because it is cheaper. In other words, it does not 
 appear to be a magical substance!

 The questions asked here are good ones. However, hopefully those reading and 
 responsible for producing procedure manuals will include the reasoning for 
 each step of the method and not just assume it is necessary, or common 
 knowledge, just because it has been handed down through generations of long 
 suffering techs!


 Tim Morken


 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
 Sent: Wednesday, July 11, 2012 7:17 AM
 To: histonet@lists.utsouthwestern.edu
 Subject: AW: [Histonet] RE: Re: Methanol in H2O2 explanation

 For the enzymatic activity of peroxidase it needs an electron-donator (or 
 receptor - I can't find the literature...) in the vicinity; therefore H2O2 
 and DAB are added ad once, and DAB is oxidized and transformed into the 
 insoluble, amorphe substance through polymerization.
 Without the donator H2O2 in excess works as inhibitor and blocks the 
 activation-side of the enzyme.
 I think H2O2 in methanol was primarly preferred, because the frozen slides 
 were fixed at the same time.

 Rehydration after dewaxing depends on the following reagens.

 Gudrun

 -Ursprüngliche Nachricht-
 Von: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Tony 
 Henwood (SCHN)
 Gesendet: Mittwoch, 11. Juli 2012 06:21
 An: 'Hobbs, Carl'; histonet@lists.utsouthwestern.edu
 Betreff: [Histonet] RE: Re: Methanol in H2O2 explanation

 Hi Carl,

 What do you mean by Why do people rehydrate after dewaxing? Do you really 
 mean that slides do not require rehydration or do you mean that slides can be 
 left to dry after de-waxing prior to staining.

 Re-hydration is necessary, otherwise xylene will prevent aqueous stains from 
 doing their thing efficiently.

 I was lead to believe that as H2O2 was catalysed by endogenous peroxidase, 
 the reactive oxygen reacted with the methanol to then degrade the enzyme, but 
 I need to look 

RE: [Histonet] Why people are having issues unsubscribing

[Connolly, Brett M]

I also agree that that adding a specific Unsubscribe link would hopefully 
alleviate the problem.

Nowplease unscribe, uscribe and unsubscribe me.

Hey - it's Friday :)

Brett M. Connolly, Ph.D.
Imaging Research Fellow
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee  Peggy Wenk
Sent: Friday, June 08, 2012 10:15 AM
To: Histonet
Subject: Re: [Histonet] Why people are having issues unsubscribing

Merced,

This makes perfect sense.  Many people (young and old) are NOT internet 
savvy and indeed will NOT go searching for the
correct way to 'unsubscribe'.

Adding a hyperlink to unsubscribe would solve this problem for most people. 
I'm certain that there are some people
that would not see something as obvious as this and would still be asking to 
be unsubscribed.

Lee Wenk

-Original Message- 
From: Leiker, Merced
Sent: Friday, June 08, 2012 9:44 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Why people are having issues unsubscribing

There are 3 reasons why it is not immediately obvious to people that one of 
the links at the bottom of Histonet email helps you unsubscribe:

1. In this day and age of internet and email hypertext clutter people simply 
do not have the time nor the desire to click and explore every link they 
come across to see which one is going to lead them to the desired page. If 
the hypertext of a link does not jump out at a person with the info they 
want, they disregard it and keep looking or freak out and ask the whole 
list. Neither link at the bottom of Histonet email explicitly states the 
info most people are probably looking for - that is, to unsubscribe.

2. Most email (junk or subscription) that people receive contain a link that 
states, To unsubscribe CLICK HERE, or words to that effect. I believe this 
is what most people are looking for who are trying to unsubscribe from 
Histonet.

3. Most people trying to unsubscribe are not even reading well-intentioned 
email sent from Histonetters trying to help them.  They see [Histonet] in 
the subject line and disregard it because they're trying to unsubscribe, not 
read more Histonet email.

Just trying to facilitate some understanding here of the problem.

That said, my suggestion to the list moderator would be to include a link at 
the bottom of Histonet email that explicitly states To unsubscribe CLICK 
HERE. This should reduce some of the clutter of unwanted and unnecessary 
off-topic (i.e., unsubscribe me please!!!) Histonet email.

Regards,
Merced

Merced M Leiker
Cardiovascular Medicine
Biomedical Research Building Rm 348
State University of New York at Buffalo
3435 Main St., Buffalo, NY  14214
(Ph) 716-829-6118
(Fx) 716-829-2665

__
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet






___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Please unsubscribe

[Connolly, Brett M]

 # 115

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bella 
Shmaltsuyeva
Sent: Thursday, May 31, 2012 11:14 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Please unsubscribe

Please unsubscribe me from this list.

Bella Shmaltsuyeva, HT (ASCP),QIHC
Senior Research Tech
Pathology Core Facility
Robert H Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-429
Chicago ,IL6061
312-503-4705
b-shmaltsu...@northwestern.edu

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: antigen retrieval

[Connolly, Brett M]

Tim- 

Antigen retrieval is off-line... you need the decloaker or other device.

Brett M. Connolly, Ph.D.
Imaging Research Fellow
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Truscott, Tom
Sent: Wednesday, March 21, 2012 4:28 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] antigen retrieval

To those with the Biocare intelliPATH system, Is the antigen retrieval part of 
the IHC automated or do you need to use the Biocare decloaker? Thankyou, Tom 
Truscott
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Congo red

[Connolly, Brett M]

I think it works better with thicker sections  ( 8-10um) 

Brett M. Connolly, Ph.D.
Imaging Research Fellow
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803



 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheri Miller
Sent: Thursday, March 01, 2012 9:28 AM
To: histonet
Cc: histonet-boun...@lists.utsouthwestern.edu
Subject: [Histonet] Congo red

Anyone have any solution to a week Congo Red? I use Rowley  Congo red, 1% 
aqueous order # S0-496. our procedure is Benholds and I cut at 5-6 microns. And 
I leave in the solution for up to 4 hours and the paths are still saying it is 
weak. Any ideas? I have even stopped the Alkaline alcohol differentiation step 
and its still too weak.

Cheryl A. Miller HT(ASCP)cm
Histology/Cytology Prep Supervisor
Physicians Laboratory Services
Omaha, NE. 402 731 4145 ext. 554



PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you 
are not the addressee intended / indicated or agent responsible for delivering 
it to the addressee, you are hereby notified that you are in possession of 
confidential and privileged information. Any dissemination, distribution, or 
copying of this e-mail is strictly prohibited. If you have received this 
message in error, please notify the sender immediately and delete this email 
from your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Any Vectra 2 users?

[Connolly, Brett M]

Histonetters,

We are contemplating upgrading our Vectra multispectral image analysis system 
to the recently released Vectra 2.

Looking for user opinions  - i.e. notice any increased speed in HPF scanning, 
improvements to software, etc.

Thanks,

Brett M. Connolly, Ph.D.
Imaging Research Fellow
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803





Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] HT/HTL Military Programs

[Connolly, Brett M]

Jennifer - 

Maybe at the Fort Sam Houston Academy of Health Sciences ?? 

I would check their AMEDD website.

Brett M. Connolly, Ph.D.
Imaging Research Fellow
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer 
MacDonald
Sent: Wednesday, January 18, 2012 1:22 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] HT/HTL Military Programs

Is anyone aware of any military based HT/HTL programs in the US?
Thank you,
Jennifer MacDonald
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Look at me...

[Connolly, Brett M]

I'll second that from our experience - Cell Signaling cleaved caspase-3 
antibody works well on xenografts

Brett

Brett M. Connolly, Ph.D.
Imaging Research Fellow
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth 
Chlipala
Sent: Thursday, January 05, 2012 12:36 PM
To: 'Sarah Dysart'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Look at me...

If you are looking to stain cleaved caspase 3 there is a better antibody from 
cell signaling it works in multiple species, it's a bit pricey but I have found 
that it works the best out of the few that I have tried.  We have used it mouse 
xenografts before without any issue.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart
Sent: Thursday, January 05, 2012 10:17 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Look at me...

I'm just full of questions today!!  This one is IHC...I have been trying to 
optimize a Caspase3 stain for several months now and it is still just chalked 
full of background gradoo.  I do all the blocking including Fc 
receptors...still junk.  The clone I have been using is, from abcam (ab2302).  
I don't see the specific clone name listed.  I am staining human xenografts 
raised in mouse.  I get a whole lot of background staining making it very hard 
to find the positive staining.  The recommended dilution is about 1:30, but I 
have diluted all the way up to 1:500.  At the higher dilution no positive 
staining or background is observed.  Does anyone know of a good Caspase3 
antibody,  preferably mouse monoclonal?  All the rabbit polyclonal antibodies 
are difficult to stain on these xenografts.
Thanks again =)

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] brain sections cut on Cryo Jane apparatus are falling off slides during ethanol series for a cresyl stain

[Connolly, Brett M]

Doug,
Hopefully you are using the CryoJane slides (not the subbed slides) and the UV 
glue activator and keeping both slides and tape cold in the cryostat. Be aware 
that you can get CryoJane slides with different amounts of adhesive (0.5-4x).  
I would suggest going to the 4x slides. When peeling the tape off I pull it 
slowly and evenly almost back on itself at an acute angle- this greatly reduces 
the incidence of the section coming off with the tape... Haven't had a problem 
with section detachment in ethanols.

Brett

Brett Connolly, Phd
Merck Research Labs
West Point, PA


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas M Burns 
[dmbur...@gmail.com]
Sent: Thursday, December 08, 2011 5:29 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] brain sections cut on Cryo Jane apparatus are falling off 
slides during ethanol series for a cresyl stain

Hello, Histo-users,

 Follow-up to our original posting about fixed-brain cryosections
falling off of subbed slides. We fished out our Cryo-Jane apparatus, and
using this apparatus has resulted in a great improvement in sections and
getting sections stuck onto the slides. This works pretty well until we
start using the ethanol series to dehydrate and delipidate before a Crystal
violet stain for Nissl bodies. (That is, for acqueous steps everything
seems to stay glued to the slide; however, once ethanols are involved
pieces start to come off.)

   To summarize, we are trying to go from cut blocks of
paraformaldehyde-fixed brain infused with sucrose and then frozen at -80.
We take them out, unfrozen, and cut them on a Leica cryostat. Early
attempts with subbed slides did not work reliably, so we have turned to
'Jane. Jane is slightly slower than a brush, and seems to work fairly well.
However, we have had trouble with the tape pulling parts of sections off of
the glued slides. When we introduce the nonacqeous solvents, our problems
become more obvious.

   We  have not so far gone to a post-fix, because we wanted to
simplify the overall process. Could  this be the mistake?

  We want to use IHC for c-Fox and possibly orexin - and similar - but
we want cresyl-stain to locate positively the area of interest in the cut
block.

   Any suggestions?  Also, are there any 'Jane enthusiasts out there?

thank  you in advance   ---   Doug
Burns, Kansas City
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Looking for a very good refurbished rotary microtome to purchase.

[Connolly, Brett M]

Bret - just google 'used laboratory equipment and you'll find a bunch of 
vendors

For example-

http://minnesotamedical.com/minnesotamedical/index.php?main_page=indexcPath=205_153
 

http://www.labx.com/v2/newad.cfm?catid=125

Cheers,

Brett M. Connolly, Ph.D.
Imaging Research Fellow
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Clough, Bret
Sent: Tuesday, December 06, 2011 11:19 AM
To: Histonet list serv.
Subject: [Histonet] Looking for a very good refurbished rotary microtome to 
purchase.

I am trying to locate a really good inexpensive rotary microtome  to purchase 
for our research lab. Any thoughts as to where to look and what model to get? 
We currently have a Lieca 1512 and are looking to have a backup. Any thoughts 
or suggestions would be greatly appreciated.

Thanks ,
Bret Clough
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Clostridium difficile - what do you do

[Connolly, Brett M]

Histonetters-

We are about to embark on a project involving C.diff infected GI tissue.

We have processes in place for PPE, decontamination/ disinfection etc... but I 
am looking for any evidence whether or not formalin fixation or subsequent 
tissue processing will inactivate the spores.

Also, do you take any special precautions in handling/sectioning the tissue 
/slides after paraffin embedding.

I am not coming up with much info with my web searches, so I am asking for help 
from any lab that works with C. diff.

Thanks,
Brett

Brett M. Connolly, Ph.D.
Imaging Research Fellow
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803





Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] pan-cathepsin antibody

[Connolly, Brett M]

Hi all-

I'm looking for a pan-cathepsin antibody for FFPE IHC and can only find Santa 
Cruz sc-6499. Has anyone used this, or other pan cathepsin antibodies?

Thanks,
Brett

Brett M. Connolly, Ph.D.
Imaging Research Fellow
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803





Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] demonstration of asbestos by means of electron microscopy

[Connolly, Brett M]

Yolanda,

Yes it is, TEM is often used for asbestos detection in lung tissue to identify 
chrysotile and/or crocidolite (mesothelioma-associated) asbestos fibers.

There are a lot of publications out there...

Brett

Brett M. Connolly, Ph.D.
Imaging Research Fellow
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803



  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yolanda Davies
Sent: Tuesday, November 22, 2011 4:14 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] demonstration of asbestos by means of electron microscopy

Dear all
 
I am a histotechnologist in forensics, Cape Town, South Africa.
I received a request to show asbestos in lung tissue where there is
definite interstitial fibrosis, but the presence of asbestos is not
clear.
 
Is it possible to reveal asbestos by means of electron microscopy?
 
Usually asbestos is demonstrated using the Perl's Prussian blue
technique, but most times they are elusive.
Could it be because of the sampling site or simply the nature of the
asbestos?
 
Thank you in advance
 
Yolanda Davies
Department of Forensic Medicine and Toxicology
Falmouth building
Anzio Road
Observatory
Cape Town 
South Africa




###

UNIVERSITY OF CAPE TOWN 

This e-mail is subject to the UCT ICT policies and e-mail disclaimer
published on our website at
http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from
+27 21 650 9111. This e-mail is intended only for the person(s) to whom
it is addressed. If the e-mail has reached you in error, please notify
the author. If you are not the intended recipient of the e-mail you may
not use, disclose, copy, redirect or print the content. If this e-mail
is not related to the business of UCT it is sent by the sender in the
sender's individual capacity.

###
 
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Antibodies for hamster tissue

[Connolly, Brett M]

Hi all ,

Wondering if anyone has experience with these antibodies on hamster tissues:

- Cathepsin B or a pan-cathepsin Ab if on exists
- MMP-3, MMP9, MMP12
- neutrophil elastase

Thanks for any info.

Brett

Brett M. Connolly, Ph.D.
Imaging Research Fellow
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803





Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: to cut or not to cut mouse brains

[Connolly, Brett M]

Terry,

Here what works for us - 

Slides are kept prechilled in a -20C freezer and then kept cold in the cryostat.

Very gently press the cold slide onto the cut section so it makes contact and 
the section sticks to the slide.

Flip slide over and use your finger to warm the back of the slide under the 
section.

Starting with cold slides is a must for us. 

Brett

Brett M. Connolly, Ph.D.
Imaging Research Fellow
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McLaughlin, 
Terry 
Sent: Thursday, November 17, 2011 2:25 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] to cut or not to cut mouse brains

Hello All,
I am having trouble cutting frozen mouse brains and was wondering if
someone can offer some help. The mouse was perfused in 4% PFA , the
brain removed and kept in PFA for 22 hrs. We placed it in 30% sucrose
for 20 hrs, until the brain sank in this solution. It was frozen back by
using a culture plate floating in liquid nitrogen with OCT . 
The problem I am having is many folds, and air bubbles under the tissue.
What I have done so far:
Cut at temp of -25, then tried - 22 and -17.
Cut at 10um, then tried 16-25um.
Tried making the knife colder by adding dry ice to it for a few seconds.
Added dry ice to sample for a couple seconds.
The sample cuts fine, it appears to happen when picking up on a slide. I
tried picking up by starting at one side or end and letting tissue
spread onto slide. Did not work.
Also tried gently laying slide on top of sample and removing
gently-still folds and air bubbles. Any help would be greatly
appreciated as we have 6 more to do.
 
Signed,
Help!
Terry Kokas
 
 
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: to cut or not to cut mouse brains

[Connolly, Brett M]

 Forgot to mention that we keep the cryostat around -16 to -18 C

Brett

-Original Message-
From: Connolly, Brett M 
Sent: Thursday, November 17, 2011 2:58 PM
To: 'McLaughlin, Terry '; histonet@lists.utsouthwestern.edu
Subject: RE: to cut or not to cut mouse brains

Terry,

Here what works for us - 

Slides are kept prechilled in a -20C freezer and then kept cold in the cryostat.

Very gently press the cold slide onto the cut section so it makes contact and 
the section sticks to the slide.

Flip slide over and use your finger to warm the back of the slide under the 
section.

Starting with cold slides is a must for us. 

Brett

Brett M. Connolly, Ph.D.
Imaging Research Fellow
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McLaughlin, 
Terry 
Sent: Thursday, November 17, 2011 2:25 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] to cut or not to cut mouse brains

Hello All,
I am having trouble cutting frozen mouse brains and was wondering if
someone can offer some help. The mouse was perfused in 4% PFA , the
brain removed and kept in PFA for 22 hrs. We placed it in 30% sucrose
for 20 hrs, until the brain sank in this solution. It was frozen back by
using a culture plate floating in liquid nitrogen with OCT . 
The problem I am having is many folds, and air bubbles under the tissue.
What I have done so far:
Cut at temp of -25, then tried - 22 and -17.
Cut at 10um, then tried 16-25um.
Tried making the knife colder by adding dry ice to it for a few seconds.
Added dry ice to sample for a couple seconds.
The sample cuts fine, it appears to happen when picking up on a slide. I
tried picking up by starting at one side or end and letting tissue
spread onto slide. Did not work.
Also tried gently laying slide on top of sample and removing
gently-still folds and air bubbles. Any help would be greatly
appreciated as we have 6 more to do.
 
Signed,
Help!
Terry Kokas
 
 
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: My bit of trivia for today - True Blue

[Connolly, Brett M]

Interesting Tim

My true blue story dates from several years back when I was working up some 
multiplex IHC staining using 2 HRP substrates.

One was DAB and the second was True Blue Substrate from KPL Labs. It worked 
quite nicely, but is alcohol/xylene soluble so one needs only to wash in water 
and air dry before coverslipping.

Follow this link for more info if interested:

http://www.kpl.com/catalog/productdetail.cfm?Catalog_ID=17Category_ID=440Product_ID=1015

Brett M. Connolly, Ph.D.
Imaging Research Fellow
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803





 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Monday, November 07, 2011 1:09 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] My bit of trivia for today - True Blue

I was listening to the Wait Wait Don't Tell Me radio show this weekend and they 
had to come up with the derivation of True Blue as a saying. Turns out it had 
to do with blue dye colorfastness. Later they would call it Fast Blue - but 
that does not convey the later meaning of the phrase..


'True blue' is supposed to derive from the blue cloth that was made at 
Coventry, England in the late middle ages. The town's dyers had a reputation 
for producing material that didn't fade with washing, i.e. it remained 'fast' 
or 'true'. The phrase 'as true as Coventry blue' originated then and is still 
used (in Coventry at least). The town's standing was recorded in 1670 by John 
Ray in the first edition of A Compleat Collection of English Proverbs:

Coventry had formerly the reputation for dying of blues; insomuch that true 
blue became a Proverb to signifie one that was always the same and like 
himself.


Tim Morken
Supervisor, Histology, IPOX
UC San Francisco Medical Center
Box 1656
1600 Divisidero St, B217
San Francisco, CA 94115
USA

415.514.6042 (office)
415.885.7409 Fax
tim.mor...@ucsfmedctr.orgmailto:tim.mor...@ucsfmedctr.org


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Luciferase IHC

[Connolly, Brett M]

Julie, 
Are you looking to stain for firefly luciferase to confirm localization of an 
optical imaging probe?

That's what I want to do, there are antibodies available from Lifespan and 
Biovision among others that are purported to work on FFPE sections I have 
yet to try them.

You can check out this article, but they used a UK supplier. 

Bioluminescence imaging to monitor bladder cancer cell adhesion in vivo: a new 
approach to optimize a syngeneic, orthotopic, murine bladder cancer model.

Jurczok A, Fornara P, Söling A.

BJU Int. 2008 Jan;101(1):120-4. Epub 2007 Sep 20.

Brett

Brett M. Connolly, Ph.D.
Imaging Research Fellow
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803




. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
Randolph-Habecker, Julie
Sent: Tuesday, November 01, 2011 6:49 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Luciferase IHC

Has anyone had good results staining for luciferase in FFPE tissue? If
so, what antibody did you use.

 

Thanks!!

 

Julie

 

Julie Randolph-Habecker, Ph.D.

Director, Experimental Histopathology Shared Resources

Fred Hutchinson Cancer Research Center

1100 Fairview Ave N, DE-360

Seattle WA 98109-1024

Tel: 206-667-6119

Fax: 206-667-6845

jhabe...@fhcrc.org

 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Anti- guinea pig polymer secondary

[Connolly, Brett M]

Hi all,

Anyone aware of any anti-guinea pig secondary HRP polymer detection kits. I 
found one from Golden Bridge Int., Inc. and am asking if anyone has used it.

Any other vendors making this??

Thanks,
Brett

Brett M. Connolly, Ph.D.
Imaging Research Fellow
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803







Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Re: Seeking Beta-Amyloid not needing FA pretreatment

[Connolly, Brett M]

Interestingwe see APP on frozen sections with 6E10, but not in paraffin 
sections after citrate HIER. We use the monoclonal from Covance, 1:12000 
overnight at 4C. 

Brett

Brett M. Connolly, Ph.D.
Imaging Research Fellow
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803



  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hobbs, Carl
Sent: Wednesday, October 26, 2011 2:52 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Seeking Beta-Amyloid not needing FA pretreatment


Be careful: HIER may expose not only extracellular amyloid plaques but also 
intracellular APP ( or...is it intracellular A beta  ;-))
Eg:  with 6E10 it is directed against an epitope found in APP and also A 
beta: if you do Formic acid, only extracellular plaques are positive...after 
HIER, there is also intracellular positivity.
In my experience.
Some examples of this can be found here, in the image gallery : 
http://www.immunoportal.com/


Whichever way you gogood luck.

Carl


Carl Hobbs
Histology Manager
Wolfson CARD
School of Biomedical Sciences
Kings College London
Guys Campus
SE1 1UL
Tel: 020 78486813
Fax: 020 78486816
020 78486813

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Seeking Beta-Amyloid not needing FA pretreatment

[Connolly, Brett M]

We have success with 6E10 and 4G8 clones using citrate retrieval.

Brett

Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A
Sent: Monday, October 24, 2011 3:39 PM
To: Histonet@lists.utsouthwestern.edu
Cc: Normington Lacy
Subject: [Histonet] Seeking Beta-Amyloid not needing FA pretreatment

Hi everyone,

I asked this question a long time ago but would like to see if anything
new is available.  We'd like to totally automate our Beta-Amyloid
antibody but in order to do so, we need an antibody that does not
require Formic Acid pretreatment.

Anyone (especially vendors) no of any on the market?

Thanks,

Linda
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Cat Scratch fever

[Connolly, Brett M]

Try Ted Nugent, Inc. ...or you can get control slides from American Master Tech.
http://www.americanmastertech.com/histology_control_slides.htm

Brett



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jessica Snay
Sent: Wednesday, October 12, 2011 1:47 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cat Scratch fever


Does anyone know where I can get a paraffin control block for cat scratch fever?
 
  
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain?

[Connolly, Brett M]

We collect EVERYTHING for recycling or disposal by our site-wide contractor 
http://www.cleanharbors.com/

Only environmentally approved hand soap and glassware cleansers are allowed 
down the drain, along with water.

Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Galbraith, Joe
Sent: Friday, September 30, 2011 10:35 AM
To: Amber McKenzie; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe 
disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain?

Amber:  Actually, no we do not dump the items you listed down the drain.  They 
get recycled appropriately.  Joe

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
Sent: Friday, September 30, 2011 9:25 AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe 
disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain?

What about Alcohol  formalin from the processor?  The diluted alcohol (100, 
95, and 75%) and the alcohol from the cleaning cycle?  Do you not pour that 
down the drain with water?  And, what about the HE stainer...do you not pour 
the bluing, hematoxylin, eosin and acid alcohol down the drain?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yaskovich, Ruth 
A (NIH/NIDCR) [E]
Sent: Friday, September 30, 2011 9:06 AM
To: Steve McClain; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe 
disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain?

Steve,
 I totally agree. NOTHING but water should go down the drain.
Ruth
N.I.H.

-Original Message-
From: Steve McClain [mailto:ste...@mcclainlab.com]
Sent: Friday, September 30, 2011 9:35 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal 
vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain?

The argument against using toxic, yet recyclable xylene in favor of a
more expensive, less efficacious xylene-substitute like Slide Bright in
larger labs is not compelling.

Aside from low volatility of Slide-Bright, what is gained in using a
more expensive substitute whose toxicities are not well-known?

The chemical composition differs, yet most MSDS warning are the same.

The advantage for low volatility can be an advantage for small hole in
the wall lab settings, like an office Mohs lab or frozen section lab in
the OR suite where ventilation may be an issue.



Unlike xylene where 75% recycling yield is norm , Slide-Bright can be
recycled- how well, I don't know.

I've requested specifics from B/R for a protocol and will forward that
info later.

However, if Slide-Bright is disposed like xylene it carries the same
disposal cost.

The company indicates Slide-Bright is a flammable aliphatic hydrocarbon
which laden with paraffin may be disposed of down the drain with copious
amounts of water, yet it is the lab directors job to ensure all local
state and federal guidelines are followed.

Aren't you defeating part of your purpose in working toward a safer lab
and greener environment by dumping aliphatic hydrocarbons into your
ground water?

Steve

Steve A. McClain, MD

McClain Labs, LLC 45 Manor Road, Smithtown, NY 11787 631 361 4000



Slide-Bright MSDS follows:

Revision Date: 6/1/2006

MATERIAL SAFETY DATA SHEET

Conforms to 93/112/EC and ISO 11014-1



1. CHEMICAL PRODUCT AND COMPANY IDENTIFICATION

PRODUCT NAME:OptiClear E
PRODUCT NUMBER:   OE-104

CHEMICAL NAMES/ DESCRIPTION:

Aliphatic Hydrocarbon



MANUFACTURER: National Diagnostics, Inc.

305 Patton Drive

Atlanta, GA 30336

TELEPHONE NUMBER: (800) 526-3867 (404) 699-2121

EMERGENCY NUMBER:

CHEMTREC (800) 424-9300

2. COMPOSITION / INFORMATION ON INGREDIENTS



Component% Comp
CAS #EINECS #  TLV (units)



Aliphatic Hydrocarbons



EEC LABEL SYMBOL AND CLASSIFICATION



R:









11-38



100



400 ppm

Highly flammable.  Irritating to skin.

S:  (2-) 16-23-24-62

Keep out of the reach of children.  Keep away from sources of ignition -
No Smoking.  Do not breathe fumes.  Avoid contact with the skin.  If
swallowed, do not induce vomiting:  Seek medical advice immediately and
show this container or label.







3. HAZARDS IDENTIFICATION

APPEARANCE AND ODOR:  Clear, colorless liquid



EMERGENCY OVERVIEW - IMMEDIATE HAZARD

HIGHLY FLAMMABLE. PRODUCT IS SLIGHTLY IRRITATING TO EYES (NO INJURY).
HIGH VAPOR MAY CAUSE RESPIRATORY TRACT 

[Histonet] Hexokinase 1 (HK1) antibody for IHC?

[Connolly, Brett M]

Hello all,

Can anybody recommend a good polyclonal anti-HK1 antibody for FFPE sections.

We want to avoid using mouse monoclonals.

Thanks much,

Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803





Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] umbilical cord - thanks

[Connolly, Brett M]

Thank you to all that offered up blocks of umbilical cord.

And  - special thanks to the 2 early responders Jim Burchette and Shirley 
Powell from whom we received the blocks last week.

Best Regards,
Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803





Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] umbilical cord block

[Connolly, Brett M]

Hi all,

Would anyone be willing to donate an FFPE block of human umbilical for a 
student I am mentoring who enrolled in an on-line histology training program?

Thanks,
Brett

Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803





Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: umbilical cord block

[Connolly, Brett M]

Hi Christine,

Here is the link for the Harford Community College on-line Histotechnology 
Certification Program.

http://www.harford.edu/cet/histotech/default.asp?FA=ContEd

Best regards,
Brett

Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803



 

-Original Message-
From: Ompoc, Marie [mailto:marie.om...@cshs.org] 
Sent: Friday, August 12, 2011 12:01 PM
To: Connolly, Brett M; histonet@lists.utsouthwestern.edu
Subject: RE: umbilical cord block

Hi all,

I'm interested to know about this online histology program? Can anybody give me 
the site? Thank you all!

Thank You,
Christine


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett 
M
Sent: Friday, August 12, 2011 8:37 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] umbilical cord block

Hi all,

Would anyone be willing to donate an FFPE block of human umbilical for a 
student I am mentoring who enrolled in an on-line histology training program?

Thanks,
Brett

Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803





Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
IMPORTANT WARNING: This message is intended for the use of the person or entity 
to which it is addressed and may contain information that is privileged and 
confidential, the disclosure of which is governed by applicable law. If the 
reader of this message is not the intended recipient, or the employee or agent 
responsible for delivering it to the intended recipient, you are hereby 
notified that any dissemination, distribution or copying of this information is 
STRICTLY PROHIBITED. If you have received this message in error, please notify 
us immediately by calling (310) 423-6428 and destroy the related message. Thank 
You for your cooperation.
IMPORTANT WARNING:  This message is intended for the use of the person or 
entity to which it is addressed and may contain information that is privileged 
and confidential, the disclosure of which is governed by
applicable law.  If the reader of this message is not the intended recipient, 
or the employee or agent responsible for delivering it to the intended 
recipient, you are hereby notified that any dissemination, distribution or 
copying of this information is STRICTLY PROHIBITED.

If you have received this message in error, please notify us immediately
by calling (310) 423-6428 and destroy the related message.  Thank You for your 
cooperation.
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] What's your favorite phospho-histone H3 antibody for IHC?

[Connolly, Brett M]

p(Ser10) or p(Ser28) ?

And have you found pHH3 (Ser28) to be more M-phase specific?

Thanks,
Brett

Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803





Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: NBF waste disposal

[Connolly, Brett M]

Sharon,

IMO - in today's world no laboratory waste should go down the drain. It is 
just not an environmentally responsible practice - and it all ends up 
eventually be discharged into in a stream somewhere. 

We collect all liquid waste (formalin, ETOH, xylenes, acids, bases, stains, 
buffers...you name it) and contract with CleanHarbors http://cleanharbors.com/  
for disposal.

Collection and proper disposal IS the best practice.

Brett

Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Campbell, Sharon
Sent: Friday, July 22, 2011 1:01 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] NBF waste disposal

Happy Friday everyone,
I am currently researching ways to dispose of NBF waste. I would like to know 
if you are neutralizing the waste and then putting it down the drain or if you 
are shipping it off to be handled by another company. I am looking for the best 
and most cost effective way to handle this waste. We are not in a position to 
reclaim the formalin as space is not available for the still.
Thank you for your help on this.

Sharon Campbell

Sharon Campbell HT, HTL (ASCP)
Histology Supervisor

Celligent Diagnostics, LLC
106 Venture Blvd.
Spartanburg, SC  29306
(864) 583-3850

Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] safe handling of tissue for histology containingradionuclides

[Connolly, Brett M]

My lab  work with tissues containing radionuclides fairly often, but
since we are in industry and not under CAP regs I can only give you some
general info.

It depends on the specific radionuclide and thus it's half-life. 

Half-lifes for common SPECT isotopes are:

I-125   60 days
Tc-99m  6 hours
In-111  2.8 days
Ga-67   3.26 days

for common PET isotopes:

F-182 hours
C-1120 min.
Cu-64   12.7 hours
Ga- 68  1.13 hours

Others you may encounter:

H-3 12 years
P-3214.3 days
P-3325.4 days
S-3587.44 days
C-145730 years

We have designated areas in the lab for working with radioactivity and
designated cryostats. 
We only process tissue with short half-lives (F-18, C-11, Cu-64, Tc99m,
Ga-68) to paraffin, and before doing so we wait 10 half-lives before
processing. 
We all wear personal dosimetry when handling tissues containing
radionuclides and that dosimetry is monitored monthly for our exposure. 
All radioactive waste, including disposable PPE, is collected in special
waste containers for proper disposal - absolutely nothing down the sink
or in regular trash.
Labs areas are surveyed with the appropriate meters/probes depending on
the radionuclide, and wipe tests are performed and wipes counted with a
scintillation counter.

That scratches the surface - others can chime in

Best regards,
Brett

Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803





  

  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of shehnaz
khan
Sent: Friday, July 08, 2011 9:30 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] safe handling of tissue for histology
containingradionuclides

Could someone please shed some light.


Hi Everyone,

Could someone kindly shed some light on safe handling of tissue for
histology containing radionuclides - to comply with CAP standards.  How
is
this done?  What should I include in the policy / work instruction?

Thanks in advance

S Kahn
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] microtome knife holder

[Connolly, Brett M]

Um, actually steel knives do in fact require a holder and disposable blades 
require a different holder, we have both from Leica.

Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, July 08, 2011 10:04 AM
To: histonet@lists.utsouthwestern.edu; Liette Tougas
Subject: Re: [Histonet] microtome knife holder

Knives (not disposable blades) do not need holders. They are used by themselves.
René J.

--- On Thu, 7/7/11, Liette Tougas ltou...@dawsoncollege.qc.ca wrote:


From: Liette Tougas ltou...@dawsoncollege.qc.ca
Subject: [Histonet] microtome knife holder
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Date: Thursday, July 7, 2011, 4:23 PM


Hi again everyone,

I also wanted to ask if anyone has, or new if there was ever, a regular knife 
(not blade) holder for the Reichert Yung 2030 microtome and/or the Leica 
microtome series.

thank you again in advance,

Liette Tougas, RT, B.Sc., M.Sc.
Biomedical Laboratory Technology Department
Dawson College
514-931-8731, ext 1519
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Luciferase

[Connolly, Brett M]

All kidding aside, assuming your talking about something tagged with
firefly luciferase you can try using monoclonal MA1-16880 from Thermo
Fisher.

Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
sgoe...@mirnarx.com
Sent: Thursday, June 30, 2011 11:55 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Luciferase

Can you stain for luciferase in tissue?

 

Sarah Goebel-Dysart, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas  78744

(512)901-0900 ext. 6912

 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] MMP-3 IHC on rat tissue

[Connolly, Brett M]

Histonetters,

Has anyone performed MMP-3 IHC on FFPE rat tissue??  I see Epitomics has
a RabMAB, but rat cross reactivity is only confirmed by Western. Would
like some suggestions from any MMP-3 users.

Thanks,
Brett

Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803




Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Toluidine blue in decaled bone

[Connolly, Brett M]

We may need to do toluidine blue staining to assess cartilage changes on
FFPE EDTA decalcified rat joints. We don't have experience w/ T blue and
I have read the EDTA will extract proteoglycans.

Help?

Thanks,
Brett

Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803



 
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Leica Cryostat Service Recommendations

[Connolly, Brett M]

I'll second that, we have 4 of these (all  6 yrs. old) and are very
happy with them.
We do not have service contracts and, when needed, have a local company
come in for repairs and PMs.

Good tip Emily about the object cooling compressor, thanks! 

Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily
Sours
Sent: Friday, April 08, 2011 3:24 PM
To: Amanda Madden; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Leica Cryostat Service Recommendations

We have the same cryostat, three years old and this is the first time
anything has broken on it.  The total cost for everything will be about
$600
which is wy cheaper than a $4000 service contract (for one year!).
Also we use our cryostat almost every day, all day.  If you're using it
that
little, and it's working fine, I would say hold off on the service
contract.  Just keep it clean and if it stops working, turn it off and
let
it defrost for a few days.  Usually that fixes everything because it's
just
iced over somewhere.
Leica service contracts are a complete waste of money--only get them if
you
need to spend money (which was the case for us a few years ago,
unfortunately, it's not now!).
Also, don't get Leica to fix your cryostat! They charge twice as much as
anyone else.
One thing to remember to save your object cooling compressor motor, when
you
restart your cryostat after defrosting, cool the chamber first, then
specimen head.  That way, the specimen head (object temperature)
compressor
will not have to work as hard to keep itself cool while the chamber is
cooling.

Emily

A great book should leave you with many experiences, and slightly
exhausted.
You should live several lives while reading it.
-William Styron



On Fri, Apr 8, 2011 at 3:11 PM, Amanda Madden amkmad...@gmail.com
wrote:

 Hello Histonetters!!!

 I am writing today to ask about the necessity of having cryostats
serviced
 regularly. We have a Leica CM 3050S that is about 2 years old now. I
have
 been looking far and wide for recommendations about cryostat
maintenance,
 but haven't found much information. Do you think it is necessary to
 purchase
 a service contract that includes having someone come out regularly to
check
 on the equipment? We love our Leica rep, but I haven't wanted to
contact
 him
 about this until I have a better idea of what other labs are doing. I
 should
 mention that ours is a very small lab, with sectioning time averaging
 only about 5 hours per week.

 Thanks for any advice!
 Amanda
 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] SOX11 antibody

[Connolly, Brett M]

Hi Martha,
I haven't used it, but you can find some info on it from the human
protein atlas here:

http://www.proteinatlas.org/ENSG0176887/antibody

They recommend 1:100 and you can check out Sigma's recommended procedure
for using their Prestige antibodies here:

http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/General_Information/
1/ihc_procedure.Par.0001.File.tmp/ihc_procedure.pdf


Brett

Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck  Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha
Ward
Sent: Thursday, March 03, 2011 1:54 PM
To: Histonet
Subject: [Histonet] SOX11 antibody

Hello all,

I have been asked to try to work up the SOX11 antibody for a research
project.   The antibody they gave me is from Sigma Life Science.  I am
having difficulty getting any staining at all.   If anyone has any
suggestions I would be very grateful.We are using a Leica Bond 3.
Thanks in advance for any help.


Martha Ward, MT (ASCP) QIHC
Assistant Manager
Molecular Diagnostics Lab
Dept. of Pathology
Wake Forest University Baptist Medical Center
Winston-Salem, NC 27157
336-716-2104

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck  Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet