[Histonet] RE: question on H pylori
Lisa, We use both IHC and Warthin Starry here, depending on the Pathologist's preference. Glen Dawson BS, HT(ASCP) QIHC IHC Manager Milwaukee, WI -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Setlak, Lisa Sent: Tuesday, June 07, 2011 1:12 PM To: histonet@lists.utsouthwestern.edu; 'histonet-requ...@lists.utsouthwestern.edu' Subject: [Histonet] question on H pylori I was just curious what everyone is using for standard of care regarding H = Pylori..is everyone doing IHC or are you doing a Giemsa? Thanks, Lisa Lisa M. Van Valkenberg, B.S., HT- ASCP Histology Manager 2300 Children's Plaza Chicago, IL 60614 773-868-8949 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC pos. neg. control question
Pete, OK, time for an example: A pathologist orders 4 IHC's on a block. I run 5 slides total: 4 IHC slides with a section of patient tissue and a known positive. 1 slide with patient tissue only for the negative control. The one negative control is put through the retrieval/protocol that is most likely to cause nonspecific staining. I don't run the known positive control tissue used on the 4 actual IHC slides as negative controls. Perhaps I didn't point out that my original post was addressing the negative control? I'm a bit surprised by the confusion. As for the question of how I know the staining seen in a positive control is truly positive?: All known positive controls are tested previously so I know that they work for the antibody that I'm using them for and I would assume the pathologist uses morphology and localization of staining to determine that the positive control is working. That's what I do. I've gone through 7 CAP inspections utilizing the practices above with no problems thus far. Perhaps you could enlighten me on IHC requirements that I haven't come across. Glen D. -Original Message- From: pete.peder...@healthonecares.com [mailto:pete.peder...@healthonecares.com] Sent: Thursday, May 19, 2011 2:31 PM To: Dawson, Glen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. neg. control question Glen, If I am to understand you correctly you are saying control tissue is not treated the same as patient tissue, therefore is useless as a negative control correct? Then inversely doesn't that mean the same thing towards the use of a positive control? How can you guarantee the positive control tissue was treated the same as the positive stained patient tissue? According to your logic it cannot. Therefore, without the use of a negative control how can you say the staining seen in the positive control is truly positive and not artifact? Best practice says use positive and negative patient and control tissue. Please enlighten me if you know anything to the contrary? Pete Pedersen B.S. HTL (ASCP) Anatomic Pathology Supervisor -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Thursday, May 19, 2011 12:32 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. neg. control question IMHO: Running any piece of tissue as a control that does not belong to the patient being tested makes zero sense. Because it would not be from the patient tissue being tested, how do you know if it was handled the same as the patient tissue? For example: 1) Were they processed the same way? 2) Did the patient tissue dry out in the OR before it was delievered? 3) Was the patient tissue ever irradiated? 4) Does the patient tissue contain any of a number of substances that could cause non-specific staining. 5) Was the patient abducted by aliens? My point is that running a piece of tissue as a negative control that is not even from the patient being tested throws all of the conditions that the patient tissue was exposed to prior to and during processing out the window. This makes NO sense. Glen Dawson BS, HT(ASCP) QIHC IHC Manager Milwaukee, WI -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Curt Tague Sent: Thursday, May 19, 2011 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC pos. neg. control question I got this email from a pathologist today. we have always run a positive with the patient tissue and a negative, the same patient tissue, and had no problems. Am I missing something. Is there any documented regulation dictating what needs to be used for the controls. In some cases if we get one slide of patient tissue, then we will use the pos. and neg. cont. from the same block but typically it's the pt. tissue that is used for the neg. control. Thanks for your guidance. Email: I received slides on sentinel lymph node biopsies with a positive control on the same slide as the breast tissue, but the negative control was just the patient's lymph node and did not have the corresponding section used for the positive control. The patient's own tissue cannot be used as a negative control. The tissue that stained positively must serve as the negative control without the antibody. This is critical and you need to correct that immediately. Curt ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman
RE: [Histonet] IHC pos. neg. control question
Tj, Amen brother! GD -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Thursday, May 19, 2011 2:39 PM To: pete.peder...@healthonecares.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. neg. control question Pete, When you run a positive control. The tissue is already a known positive (or it should be) for whichever antibody you are running regardless of prior handling. It would be impossible for this not to be so. However, with a negative, the concern is seeing how the patient tissue turns out when subjected to all the same conditions, minus the antibody. tj -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of pete.peder...@healthonecares.com Sent: Thursday, May 19, 2011 12:31 PM To: gdaw...@dynacaremilwaukee.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. neg. control question Glen, If I am to understand you correctly you are saying control tissue is not treated the same as patient tissue, therefore is useless as a negative control correct? Then inversely doesn't that mean the same thing towards the use of a positive control? How can you guarantee the positive control tissue was treated the same as the positive stained patient tissue? According to your logic it cannot. Therefore, without the use of a negative control how can you say the staining seen in the positive control is truly positive and not artifact? Best practice says use positive and negative patient and control tissue. Please enlighten me if you know anything to the contrary? Pete Pedersen B.S. HTL (ASCP) Anatomic Pathology Supervisor -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Thursday, May 19, 2011 12:32 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC pos. neg. control question IMHO: Running any piece of tissue as a control that does not belong to the patient being tested makes zero sense. Because it would not be from the patient tissue being tested, how do you know if it was handled the same as the patient tissue? For example: 1) Were they processed the same way? 2) Did the patient tissue dry out in the OR before it was delievered? 3) Was the patient tissue ever irradiated? 4) Does the patient tissue contain any of a number of substances that could cause non-specific staining. 5) Was the patient abducted by aliens? My point is that running a piece of tissue as a negative control that is not even from the patient being tested throws all of the conditions that the patient tissue was exposed to prior to and during processing out the window. This makes NO sense. Glen Dawson BS, HT(ASCP) QIHC IHC Manager Milwaukee, WI -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Curt Tague Sent: Thursday, May 19, 2011 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC pos. neg. control question I got this email from a pathologist today. we have always run a positive with the patient tissue and a negative, the same patient tissue, and had no problems. Am I missing something. Is there any documented regulation dictating what needs to be used for the controls. In some cases if we get one slide of patient tissue, then we will use the pos. and neg. cont. from the same block but typically it's the pt. tissue that is used for the neg. control. Thanks for your guidance. Email: I received slides on sentinel lymph node biopsies with a positive control on the same slide as the breast tissue, but the negative control was just the patient's lymph node and did not have the corresponding section used for the positive control. The patient's own tissue cannot be used as a negative control. The tissue that stained positively must serve as the negative control without the antibody. This is critical and you need to correct that immediately. Curt ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC pos. neg. control question
IMHO: Running any piece of tissue as a control that does not belong to the patient being tested makes zero sense. Because it would not be from the patient tissue being tested, how do you know if it was handled the same as the patient tissue? For example: 1) Were they processed the same way? 2) Did the patient tissue dry out in the OR before it was delievered? 3) Was the patient tissue ever irradiated? 4) Does the patient tissue contain any of a number of substances that could cause non-specific staining. 5) Was the patient abducted by aliens? My point is that running a piece of tissue as a negative control that is not even from the patient being tested throws all of the conditions that the patient tissue was exposed to prior to and during processing out the window. This makes NO sense. Glen Dawson BS, HT(ASCP) QIHC IHC Manager Milwaukee, WI -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Curt Tague Sent: Thursday, May 19, 2011 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC pos. neg. control question I got this email from a pathologist today. we have always run a positive with the patient tissue and a negative, the same patient tissue, and had no problems. Am I missing something. Is there any documented regulation dictating what needs to be used for the controls. In some cases if we get one slide of patient tissue, then we will use the pos. and neg. cont. from the same block but typically it's the pt. tissue that is used for the neg. control. Thanks for your guidance. Email: I received slides on sentinel lymph node biopsies with a positive control on the same slide as the breast tissue, but the negative control was just the patient's lymph node and did not have the corresponding section used for the positive control. The patient's own tissue cannot be used as a negative control. The tissue that stained positively must serve as the negative control without the antibody. This is critical and you need to correct that immediately. Curt ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ventana's Basal Cell Cocktail
Sharon, I do a CK34BE12 P63 cocktail for basal cells charge for only one stain. That being said, you can absolutely charge for 2 as P63 in nuclear the CK34BE12 is cytoplasmic, so the pathologist can discern one from the other. I am considering setting it up as a double charge in the future if our volume for it continues to climb as it has been lately. Glen Dawson BS, HT(ASCP) QIHC IHC Manager Milwaukee, WI -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Wednesday, March 30, 2011 11:10 AM To: Sharon.Davis-Devine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ventana's Basal Cell Cocktail Sharon, We're actually doing a triple stain of HMWCK + p63 + p504S but charging as a single stain. I am advocating charging for at least 2 of the 3 stains (done in 2 separate runs) since one can easily differentiate between 2 different chromogen colors. The jury's still out. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Wednesday, March 30, 2011 9:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana's Basal Cell Cocktail To all of you Histonetter's out there I need to know for those of you that are using the new Ventana antibody, CK34BE12 + p63, Basal Cell Cocktail, are you charging these as as one charge or two? Any information will be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-dev...@carle.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Herpes Virus
All, I am looking for a good HSVI/HSVII cocktail for IHC. Can anyone out there suggest a good one? Thank-you in Advance, Glen Dawson BS, HT(ASCP) QIHC IHC Manager Milwaukee, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] High Complexity Testing
Sheila, Yes, IHC/ISH is considered high complexity testing. Sounds like you have a real gem for a pathologist...sorry to hear that. Just don't let him/her pay you like a janitor just because that's what he/she thinks that is what you are. Glen Dawson BS, HT(ASCP) QIHC IHC Manager Milwaukee, WI -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner Sent: Tuesday, February 08, 2011 6:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] High Complexity Testing Hello All, I would really appreciate it if anyone has information on whether IHC/ISH are considered high complexity testing for histotechs. Our pathologist believes that ALL histology low complexity testing since a machine is doing the work. Can anyone help me out with some guidelines, literature, etc. that says otherwise? I would really appreciate it. We just want to know which one it is. Thanks so much Histoland! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] High Complexity Testing
Sorry for the early morning grammar everyone... -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Tuesday, February 08, 2011 7:13 AM To: 'Sheila Fonner'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] High Complexity Testing Sheila, Yes, IHC/ISH is considered high complexity testing. Sounds like you have a real gem for a pathologist...sorry to hear that. Just don't let him/her pay you like a janitor just because that's what he/she thinks that is what you are. Glen Dawson BS, HT(ASCP) QIHC IHC Manager Milwaukee, WI -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner Sent: Tuesday, February 08, 2011 6:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] High Complexity Testing Hello All, I would really appreciate it if anyone has information on whether IHC/ISH are considered high complexity testing for histotechs. Our pathologist believes that ALL histology low complexity testing since a machine is doing the work. Can anyone help me out with some guidelines, literature, etc. that says otherwise? I would really appreciate it. We just want to know which one it is. Thanks so much Histoland! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] High Complexity Testing
Alright, if IHC is not high complexity testing, CAP should cut that massive part of their inspection in half and concentrate more on the pathologists' ability to accurately interpret the staining. Too much CAP regulation, Proficiency Testing validation requirements involved if all IHC is is part of Processing. My Opinion, Glen A. Dawson Milwaukee, WI -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Tuesday, February 08, 2011 12:17 PM To: Whitaker, Bonnie Cc: Horn, Hazel V; Goins, Tresa; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] High Complexity Testing I should not have included CLIA in my e-mail as it would seem it has clouded things a little. I do apologize. Initially when these issues and guidelines came about CLIA and CAP dovetailed as far as Histology was concerned. Shelia you were looking for contacts that would help you with getting a more solid base to meet these regulations. If you go to the CAP website and click on the IHC link you will find links and publications to assist you. I would recommend that you contact the Applied Immunohistochemistry society as well. NSH or your state/regional society may also have additional information. Should I see something else in my searches I will most willingly forwarded them to you. Vikki On Tue, Feb 8, 2011 at 12:43 PM, Whitaker, Bonnie bonnie.whita...@osumc.edu wrote: Hi All, There is a difference in performing a task (immunostaining) that is complex, and performing high complexity testing as the CLIA regulations govern. Yes, staining is a complex task, and it requires knowlegable techs to ensure that it is properly done, and to troubleshoot difficulties when necessary. It is high complexity testing because testing personnel in anatomic pathology are pathologists (and the non-physician people performing gross examinations, who must meet high complexity testing personnel requirements. Testing personnel as defined by CLIA, are the people that report results of that test, not people who perform other related duties. That's my explanation of the whole mess. Bonnie Whitaker AP Operations Director Ohio State University Medical Center Department of Pathology 614.293.5048 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Tuesday, February 08, 2011 12:22 PM To: Horn, Hazel V; 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; Sheila Fonner Subject: RE: [Histonet] High Complexity Testing I must disagree with this assessment of what makes a test complex. If the test is done properly [the responsibility of the technologist] then the reading to the test is a visual determination that requires experience on the part of the pathologist, but if the test is not done properly, will the pathologist be able to tell the technologist what to do to fix the problem? Where's the Tylenol? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Tuesday, February 08, 2011 9:58 AM To: 'Rene J Buesa'; histonet@lists.utsouthwestern.edu; Sheila Fonner Subject: RE: [Histonet] High Complexity Testing While the test is high complexity it is the READING of the test by the pathologist that determines its complexity. Because histotechs do not report the results our part of this test is not high complexity. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's WaySlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, February 08, 2011 10:32 AM To: histonet@lists.utsouthwestern.edu; Sheila Fonner Subject: Re: [Histonet] High Complexity Testing When a machine is doing the test, there are stringent provisions as to the preparation and validations of the test. Done manually, it requires a trained technologists and, yes, they are high complexity tests (both IHC and FISH, and their variations). René J. --- On Tue, 2/8/11, Sheila Fonner sfon...@labpath.com wrote: From: Sheila Fonner sfon...@labpath.com Subject: [Histonet] High Complexity Testing To: histonet@lists.utsouthwestern.edu Date: Tuesday, February 8, 2011, 7:45 AM Hello All, I would really appreciate it if anyone has information on whether IHC/ISH are considered high complexity testing for histotechs. Our pathologist believes that ALL histology low complexity testing since a machine is doing the work. Can anyone help me out with some guidelines, literature, etc. that says
[Histonet] OCT 3/4
All, My docs are asking that I work up an OCT 3/4 antibody. Can anyone give me their opinion on a good one? I'm not finding a stand out in my research so I'm looking for some opinions from those in the trenches. Thanx in Advance, Glen Dawson BS, HT(ASCP), QIHC IHC Manager Milwaukee, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: [Histonet] Posting comments
-Original Message- From: Dawson, Glen Sent: Monday, September 20, 2010 9:10 AM To: 'Merced M Leiker' Subject:RE: [Histonet] Posting comments Carrie, Until you've had some psychopath threaten you with a lawsuit, or threaten to tell on you with your current employer, it will be difficult to understand why most of us choose to reply directly to whomever posts the question. I've had this happen to me on this list because someone out their did not agree with my general post needed to act like a child to get his point across. I've responded directly to the person that posts the question ever since...basically to avoid the hassle. I realize that this isn't the best situation for the distribution of useful information, but all of us need to remember that there will always be a small percentage of bad apples in the basket that is the histonet and that those of us that won't hit reply to all don't do so of our own accord. Glen Dawson BS, HT QIHC (ASCP) IHC Manager Milwaukee, WI -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Merced M Leiker Sent: Monday, September 20, 2010 8:45 AM To: Carrie Disbrow; histonet@lists.utsouthwestern.edu Subject:Re: [Histonet] Posting comments Agreed. * On Saturday, September 18, 2010 1:25 PM -0400 Carrie Disbrow cdisb...@msn.com wrote: As a newbie trying to learn as much as possible, I'm disappointed when questions are posted and the responses are sent to the individual. I learn more when the responses are shared. Also, an archive search on the subject will have limited information. I would think there are many in the same boat - those who want to learn more about the craft. Even a review of the basics is good reading for me. That is why I reply all instead of reply. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: AMT Cover
All, I just looked at the notorious AMT cover that people are up in arms about and I must disagree with the comments that were posted thus far. The techs here and myself are quite surprised by the reactions because it is on a medical catalogue and is far from being lewd or obscene. You could probably find worse by turning on the TV for 15 minutes or looking at a billboard or two. Just Some Wisconsin Input, Glen Dawson BS, HT QIHC (ASCP) IHC Manager Milwaukee, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CD68
I use both KP-1 and PGM-1. KP-1 is ordered about ten times as much as the PGM-1. Glen Dawson BS, HT QIHC (ASCP) IHC Manager Milwaukee, WI -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Friday, April 30, 2010 10:49 AM To: 'Histonet' Subject:[Histonet] CD68 Happy Friday Everyone What clone is everyone using for the CD68 antibody for FFPE human tissue? Thanks for the info in advance. Everyone have a great weekend. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: CD5
Jeff, Thermo Scientific (Formally NeoMarkers) CD5 (Clone SP19): Catalogue# RM-9119-S. Concentration of 1:75 diluted w/Dako diluent. Antigen Retrieval: PH6.0 Citrate Target Retrieval (DAKO) for 35 min. @ 99 degrees C, followed by a 20 min. room temp. cool down. 35 minute primary antibody application. 30 minute Envision+ polyclonal (DAKO) secondary antibody detection. 7 minute DAB+ (DAKO). Counter-stain as you like. Results on this antibody are excellent. Best of Luck, Glen Dawson BS, HT QIHC (ASCP) IHC Manager Milwaukee, WI From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Howery, Jeffrey [jhowe...@yrmc.org] Sent: Friday, April 02, 2010 4:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD5 Can anyone share thier protocol for CD5. We use the Dako platform. Thanks Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Histo Day
I was working at a grocery store deli with a BS in biology when a teacher from the local technical college started there for something to do over the summer. He told me about a program called Histotechnology that boasted 100% placement after graduation. That was enough for me I was interning in Milwaukee, WI two years later. I don't think anyone ever sets out to be a histotech, we all just sort of fall into it. Happy HP Day All, Glen Dawson BS, HT QIHC (ASCP) IHC Manager Milwaukee, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Slide marking pens
I have had very good luck with the Surgipath Marking Pens Cat.#01880. I've tried many they are the only ones that can stand up to my high pH Heat Induced Epitope Retrieval (HIER). Best of Luck, Glen Dawson BS, HT QIHC (ASCP) IHC Manager Milwaukee, WI -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Wednesday, January 13, 2010 2:50 PM To: Mike Pence; Weems, Joyce; histonet@lists.utsouthwestern.edu Subject:RE: [Histonet] Slide marking pens I have a question re: these pens. We are a research lab that does histology for our group. We mark our cassettes and slides with special marking pens (currently Statmark Pen), but we are having a problem with the ink wearing off after processing. We have tried marking over the info repeatly, but the ink still comes off! Are these pens for this purpose? Or can you recommend a good marking pen for us? Thanks in advance. Peggy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, January 13, 2010 3:02 PM To: Weems, Joyce; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide marking pens Thanks for all the rapid replies. Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, January 13, 2010 1:53 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide marking pens I order these from Staples, formerly Corporate Express. Pens, xylene free marker, black DZ PIL44102 Pens, xylene free marker, greenDZ PIL44103 Pens, xylene free marker, red DZ PIL44104 Pens, xylene free marker, blue DZ PIL44105 Hope this helps! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, January 13, 2010 14:37 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide marking pens I am wondering if anyone can tell me where to get slide marking pens that the Pathologist would mark an area of interest on the cover slipped side of a slide. Cytologist use these all the time. hey most often have blue ink. Thanks for any help I can get. Mike ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE:Missing the point of Plants-in-the-lab OT
All, We as human beings are sloughing off skin cells, germs, hair, etc... Since we could contaminate the lab, I move that all people be banned from the histo lab...oh yeah, how would the work get done? Long story short, it is correct that plants can cause contamination, but it is overkill to ban them from the lab since almost anything could be considered a possible contaminant. All things that may give lab workers joy, peace or general enjoyment need not be banned. Maybe in a couple years, the No Fun No Happiness agenda can be put in place, but for now I will enjoy looking at our plants. Cheers, Glen Dawson IHC Manager Milwaukee, WI -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Galbraith, Joe Sent: Thursday, October 22, 2009 2:55 PM To: McMahon, Loralee A; Akemi Allison-Tacha; Sara''Breeden; histonet@lists.utsouthwestern.edu; LindaBlazek; Patti Loykasek Subject:RE: [Histonet] RE:Missing the point of Plants-in-the-lab OT All: We had an inspector comment that plants were discouraged only because of the possibility that the plant pollens or the microbes on the plants or in the soil could end up on the water bath (and other work surfaces) and hence ultimately (directly or indirectly) in the tissue on the slides where the pathologist may have to determine if they were contaminants or integral to the tissue. Most of the time, this would be trivial but in some cases the issue could be substantive. So we were told. Sadly, we no longer have plants in the lab despite their positive impact on air quality and employee satisfaction. Joe joseph-galbra...@uiowa.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McMahon, Loralee A Sent: Thursday, October 22, 2009 2:31 PM To: Akemi Allison-Tacha; Sara''Breeden; histonet@lists.utsouthwestern.edu; LindaBlazek; Patti Loykasek Subject: RE: [Histonet] RE:Missing the point of Plants-in-the-lab OT I just want to know what CAP question states that you cannot have plants in the lab. Or is this the inspectors interpretation of a CAP question. Maybe the question regarding the lab working conditions. I could see if you have plants all over the counters crowding the space. But that is not regarding the plants themselves that would be in regard to having a crowded work environment. ?? When you give a phase 1 deficiency you have to reference the CAP question. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha [akemiat3...@yahoo.com] Sent: Thursday, October 22, 2009 3:26 PM To: Sara''Breeden; histonet@lists.utsouthwestern.edu; LindaBlazek; Patti Loykasek Subject: Re: [Histonet] RE:Missing the point of Plants-in-the-lab OT Hi All, I think all of you are missing the point of Patti's question. She stated that her lab was dinged for having plants in the lab by a CAP inspector. I had the same thing happen to me years ago. The inspector stated that plants attract insects that can contaminate a supposedly clean environment. Patti has an extremely well organized lab that only had a small phase (1) deficiency last year. I think the inspector couldn't find anything, so they had to come up with this ridiculous infraction. Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingTele: 408.402.5257 Cell: 408.335.9994 E-Mail: akemiat3...@yahoo.com --- On Thu, 10/22/09, Blazek, Linda lbla...@digestivespecialists.com wrote: From: Blazek, Linda lbla...@digestivespecialists.com Subject: [Histonet] RE: Plants-in-the-lab OT To: 'Breeden, Sara' sbree...@nmda.nmsu.edu, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Thursday, October 22, 2009, 12:15 PM I don't know Sally, but where I worked many moons ago I had a spider plant that was extremely prolific. The powers that be made me remove it from the lab for an inspection. It went to live in one of the administrator's office for several months. And died! I think it needed the fumes! Or it missed me. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Phone: (937) 293-4424 ext 7118 Email: lbla...@digestivespecialists.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, October 22, 2009 3:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Plants-in-the-lab OT I think it's the fluorescent lights that makes them thrive. The absorption of Fume Matter is a secondary, but beneficial, effect. You go, chlorophyll! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic
[Histonet] C3d
All, I have a request to work up a monoclonal C3d fluorescent stain (for frozen sections). Can anyone out there suggest a good one? I am currently running C4d clinicians are asking for the C3d as well. Thank-you in Advance, Glen Dawson BS, HT QIHC (ASCP) IHC Manager Milwaukee, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IgG4
All, Is anyone performing paraffin block IHC staining for IgG4? If so, a vendor name would be much appreciated. Thank-you, Glen Dawson Milwaukee, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Lab Telephone
Sara, Maybe you're looking a gift horse in the mouth. There have been many days that I WISHED that I had no phone in my lab because it was a constant distraction in an already hectic day. Once your boss has to hand deliver a few messages/orders to you, he/she will most likely provide a phone for you without the need for a justification. Just a Thought, Glen Dawson IHC Manager Milwaukee, WI From: Breeden, Sara sbree...@nmda.nmsu.edu Subject: [Histonet] Lab Telephone To: histonet@lists.utsouthwestern.edu Date: Tuesday, August 18, 2009, 3:48 PM I'm having a challenge wrapping my head around this one: my boss wants to know why I need a telephone in my new lab in the new building we're moving into in January. Part of this is cost (what isn't a cost issue these days??) but I am to come up with justification for having a telephone IN the lab. The new phones will be cordless but he wants the phone to be in the hallway for what he deems a safety issue (I am not a BSL3 lab). The new phone system will be individual lines for specific locations within our facility. I'm the only tech and I'll have a 22x22' lab and the adjoining (with separate entrance) storage room/volatile storage and I will be doing specimen cut-in in another area altogether. I know I've left out some salient points so if you need more info AND if you have some logical input, please ANSWER offline to me directly. I can't figure out why I would NOT need a telephone... egad! I was asked how many calls (in/out) I make/receive per month as a basis for the decision. OMG. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Special stainers
The Artisan (DAKO) is the best. Glen Dawson BS, HT QIHC (ASCP) IHC Manager Milwaukee, WI -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Tuesday, July 14, 2009 9:13 AM To: histonet Subject:[Histonet] Special stainers What stainer is everyone liking the best? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Off topic posts
KIDS, KIDS!!! Enough is enough. Take it off the list and respond to one another privately. It's fast becoming like observing a daycare here. Glen Dawson IHC Manager Milwaukee, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Biomeda Corp.
All, I vaguely remember a discussion on this, but could someone please refresh me on who bought out Biomeda and perhaps provide contact information for whomever is now dispensing their product? I am looking for their FITC Streptavidin (F72) so if there are any vendors that provide a similar item, please feel free to respond directly to me so I have some options for if it is discontinued all together. On another note, I'm also using Biomeda's Crystal Mount Aqueous Mounting media so alternative product suggestions for this one would be much appreciated as well. Thank-you in Advance, Glen Dawson BS, HT QIHC (ASCP) IHC Manager Milwaukee, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
