RE: [Histonet] Input on Sakura E150 Purchase
Another +1 for the VIP E300 (very similar model). We have one that's close to 20 years old that's still running. It has an annual PM and any repairs have been minor. I have been told that part availability isn't yet an issue with this model. Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -Original Message- From: margaret chadwell [mailto:m_chadw...@hotmail.com] Sent: Wednesday, February 26, 2014 4:57 PM To: histonet@lists.utsouthwestern.edu; histonet-requ...@lists.utsouthwestern.edu Subject: [Histonet] Input on Sakura E150 Purchase Sent from Windows Mail Hello Histonet, Does anyone have input on the Sukura E150 tissue processor? Our research lab is looking at purchasing a used model from Rankin Biomedical, but we were concerned about the age of the unit, 17 years old. They mentioned it is totally refurbished, will give a one year warranty, say it’s a reliable unit, and parts are available for servicing. Any info or suggestions would be greatly appreciated. Like so many we are on a limited budget so we can’t buy new. Thank you in advance! Margaret Chadwell LIAI La Jolla, Ca _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cell Block Preparation
We have had mixed results in the past with cell blocks, but currently we are filtering the fluid through a biopsy bag and processing that. We seem to be retaining more specimen with that method (we have used sponges and lens paper in the past). Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -Original Message- From: Ann Specian [mailto:thisis...@aol.com] Sent: Thursday, September 05, 2013 12:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cell Block Preparation I am getting complaints in regard to insufficient cell blocks. We currently spin, pour off the supernatant, retrieve the sediment and process in lens paper. Does anyone have a more current technique which renders better cellularity? Also, do you know which renders a better cell block: a fresh specimen, a specimen fixed in Cytolyt or a specimen fixed in 10% NBF? Thanks, Ann _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Hardware from surgery
We retain for two years post sign-out. If the patient is a minor, then until their 18th birthday plus two years. Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -Original Message- From: anita dudley [mailto:azdud...@hotmail.com] Sent: Friday, August 02, 2013 12:33 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Hardware from surgery What do others do with hardware from surgery? Do you hold on to it for a longer period of time because of a possible legal case? Thanks so much, everyone have a great weekend!! Anita Dudley Providence Hospital Mobile, Al. 36695 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC on non-charged slides
I'm hoping Histonet can come through for me! I have an unusual case that I need to run IHC on. Antibody is Pan Keratin Cocktail from Cell Marque with recommended protocol on a Benchmark Ultra with i-view detection. Only problem is, I only have two slides and they are not charged. The tissues have been air-dried but not baked. I cannot obtain more slides. I'm wondering if there's any kind of pre-treatment I could try to try to help the tissue stay on. We have run slides like this in the past with mixed results. Most of the time, the tissue washes off. Any tips or tricks that might help out in this situation? Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Histonet Digest, Vol 113, Issue 30
Surgical Pathology Technologist Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -Original Message- From: Barr,Kaye H [mailto:khb...@mdanderson.org] Sent: Tuesday, April 30, 2013 1:49 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Histonet Digest, Vol 113, Issue 30 What title do most use for someone who does gross and is not a PA? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Tuesday, April 30, 2013 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 113, Issue 30 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. BIG PROBLEM (Behnaz Sohrab) 2. Re: BIG PROBLEM (Linda) 3. Yearly Training (Nancy Schmitt) 4. HIER pressure cookers (k. leigh adams) 5. RE: HIER pressure cookers (Connolly, Brett M) 6. RE: HIER pressure cookers (Blazek, Linda) 7. Re: HIER pressure cookers (Jan Shivers) 8. RE: 5 gal specimen container search (Hannen, Valerie) 9. RE: 5 gal specimen container search (Shirley A. Powell) 10. RE: HIER pressure cookers (dingers...@aplaboratories.com) 11. AW: [Histonet] BIG PROBLEM (Gudrun Lang) 12. Processor question - alcoholic formalin vs. buffered formalin (Lake, Kim S) 13. RE: Processor question - alcoholic formalin vs. buffered formalin (Hannen, Valerie) 14. cassette labelers (Margiotta-Watz, Michele) -- Message: 1 Date: Mon, 29 Apr 2013 10:36:01 -0700 From: Behnaz Sohrab sohra...@ah.org Subject: [Histonet] BIG PROBLEM To: histonet@lists.utsouthwestern.edu Message-ID: 517e4d11.4347.005...@ah.org Content-Type: text/plain; charset=us-ascii Good Morning, We have two Leica HE stainer( old xt-5010 and new 5020). We do use : Gill's 3 for 6 minutes, 4% Glacial ACETIC Acid 40 seconds Bluing : 45 sec. We done this for years!! last few months our Pantologists are complaining about GI Biopsies that they have TOO much blue in cytoplasma and the Mucin is too purple and blue? Please any suggestion?? Mean while we are trying the NEW kit from Leica for HE , they think that is TOO pale?any of you has tried this new stain from Lecia? With much application. Behnaz Sohrab, Los Angeles -- Message: 2 Date: Mon, 29 Apr 2013 11:30:38 -0700 (PDT) From: Linda lmd...@yahoo.com Subject: Re: [Histonet] BIG PROBLEM To: Behnaz Sohrab sohra...@ah.org, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 1367260238.82176.yahoomail...@web164002.mail.gq1.yahoo.com Content-Type: text/plain; charset=iso-8859-1 Hi, ? I would first check with your alcohol and xylene suppliers to see if they changed anything.? When something?goes south it is usually because the manufacturer have changed.? This happened with my silver stains. ? You may have to go back to the basics- harris hematoxylin, acid alcohol, and eosin y.? Richard Allen 7211 hematoxylin and the rest of their staining is excellent in these situations until you can get it back under control.? If you send them slides they will work a time out for you. ? If you are using Leica's selectech, which is what I started this lab out with because I was heavily sampled out,?it has blue nuclei instead of the traditional purple.? I do 5 minutes in hematoxylin, 30 dips in clarifier and 1 minute in blue, 55 sec in eosin but I have (2) 95s after.? They also have a tech dept to help.? I have a Leica autostainer too. ? I hope this helps. ? Linda Dee, HT(ASCP) ? From: Behnaz Sohrab sohra...@ah.org To: histonet@lists.utsouthwestern.edu Sent: Monday, April 29, 2013 12:36 PM Subject: [Histonet] BIG PROBLEM Good Morning, We have two Leica HE stainer( old xt-5010 and new 5020). We do use : Gill's 3 for 6 minutes, 4% Glacial ACETIC Acid 40 seconds Bluing : 45 sec. We done this for years!! last few months our Pantologists are complaining about GI Biopsies that they have TOO much blue in cytoplasma and the Mucin is too purple and blue? Please any suggestion?? Mean while we are trying the NEW kit from Leica for HE , they think that is TOO pale?any of you has tried this
[Histonet] SP and CGRP antibodies
Hello everyone, Is anyone out there using a commercially available antibody for SP (Substance P) and CGRP (Calcitonin gene-related peptide)? If so, where do you obtain it? Thanks! Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] WT1 overstaining on Benchmark Ultra
Hello Histonet, I have two strange occurrences that I'm trying to get to the bottom of. Running WT1 from Cell Marque on a Benchmark Ultra using iView detection and the recommended protocol. Two specimens (both of which happen to be pleural fluid) severely overstained, even leaving the slide with a brown background stain. This has only happened twice, and the slides were on different positions on different staining runs. They used the same antibody and detection kit, however other pleural fluid slides with WT1 are fine. Other slides that run on the same positions also stain ok. I am repeating the stains on the same tissues to try to repeat this but in the meantime has anyone experienced this condition or have any advice? The only time I've seen this condition before was due to a clogged vortex mixer, but it affected all the slides and is obviously not the case here. Thanks! Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] tissue processor remote alarm systems
I have a Sensaphone unit. It can handle four different instruments. When it calls it will give you the phone number it's calling from, the sensor (instrument) number, and the 'alert condition' (there are several...for example 1 is power failure, etc.) Any other questions about it I'm happy to help. Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -Original Message- From: Morken, Timothy [mailto:timothy.mor...@ucsfmedctr.org] Sent: Thursday, February 14, 2013 11:01 AM To: Histonet Subject: [Histonet] tissue processor remote alarm systems I'd like to hear from labs that use a remote messaging system for their VIP5 tissue processors that are left unattended overnight. One I saw on old histonet posts is Sensaphone. I am wondering what kind of information you can get from the system. Does it just say there is a problem but no details? Can you connect several processors to one sensing unit? Can it tell the difference between the units (ie, processor 1 vs processor 4?). Thanks for any info! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Xylene Substitutes
We used SubX but have switched to Clear-Rite 3 from Thermo Fisher with excellent results as well as cost savings. Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -Original Message- From: Adrienne Anderson [mailto:rennie1...@yahoo.com] Sent: Tuesday, February 05, 2013 4:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Substitutes Hello all, My lab is looking into xylene substitutes, and I'd love some feedback on what other labs are using. We currently use SubX, but are there other items out there more economical? Thanks, Adrienne _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Charges for cutting research case
We have worked out a cost for cutting and staining HE's, so we basically take that cost and mark it up. Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -Original Message- From: Cheri Miller [mailto:cmil...@physlab.com] Sent: Wednesday, November 07, 2012 12:44 PM To: histonet-boun...@lists.utsouthwestern.edu Cc: histonet Subject: [Histonet] Charges for cutting research case I would like some direction in regards to how to charge a client for cutting multiple slides and levels anywhere between 10 to 50 slides per block of a Technical component case. These would be research cases. Any help directing me would be greatly appreciated. Thank, Cheri Cheryl A. Miller HT(ASCP)cm Histology Supervisor, Hygiene Officer Physicians Laboratory Services 4840 F Street Omaha, NE. 68127-0999 402 731 4145 ext. 554 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Number of blocks
I find it interesting that this issue comes up every so often on Histonet. There are so many factors that affect the time it takes to get a quality section that it seems silly to set an arbitrary number that techs are expected to reach. In my opinion, this is trying to find a way to measure productivity on a process that has too many variables to be accurately measured. I can understand the desire to find a way to measure this productivity, but my number one rule is still quality over quantity. Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -Original Message- From: Dorothy Ragland-Glass [mailto:techman...@yahoo.com] Sent: Wednesday, October 24, 2012 8:38 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Number of blocks It was annouced by a histo lab manager that techs are expected to cut 40-50 blocks per hour. That seems to me to be rather high. I don't see quality slides being turned out. It is quantity and profit above patient care. I am old school, and I remember something about quality and patient first. Besides what kind of impact on morality of the techs, back problems and carpal tunnel syndrom is laying ahead for the cutter after cranking the microtome repeatedly that many blocks without a break. _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] negative controls
You may be able to omit them, but in our case they are still a detection that is part of our volume agreement. I'm not sure if everyone else has a similar agreement, but omitting the negative controls may affect your ability to meet a volume commitment. Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -Original Message- From: Vanessa Perez [mailto:vpe...@pathreflab.com] Sent: Thursday, August 02, 2012 8:35 AM To: anita dudley; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] negative controls Yes you can, the cap standards just came out Tuesday with the revised part stating you do not have to run a negative reagent control using polymer based detection. Only if you are still using biotin based detection kits do you need to run the neg. control Vanessa Perez Garcia Histology Supervisor Pathology Reference Lab 210-892-3746 210-892-3732 vpe...@pathreflab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Wednesday, August 01, 2012 4:13 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] negative controls just wondering if we can start leaving the negative controls off of our immuno stains now as long as our procedure says we can stain without them? we use ventanas multimer detection systems. thanks so much, anita dudley providence hospital mobile, alabama ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cost analysis
Hello all, I've been asked to update some dated test cost information. I need to come up with a a total cost (materials, burden [common materials] and labor) for IHC, ISH, special stains, and routine HE (to include processing, embedding, cutting, staining). I'm not too concerned with IHC and special stains, but I'm hoping to get some input regarding routine HE and associated work because there's a lot of factors to consider and many are variable. Has anyone done this, and would mind providing some thoughts on this process? Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC Validation
Hello all, A year or so ago, we upgraded from a Benchmark XT to Benchmark Ultra. For validation, we selected a variety of cases as usual and ran them on both instruments before we retired the XT. Now, when we add a brand new antibody, we again select a variety of cases, and once we're happy with them on the Ultra we send the same cases to a reference lab for comparison. I'm now faced with changing clones for several antibodies. I expected to go through pretty much the same validation procedure, but it got me thinking...the reference lab does not always use the same clone as some of ours. I suppose this really wouldn't be a 'true' validation in this case. Does anyone have any thoughts on this? The pathologists are perfectly happy with the staining of the new clone, but the only reference lab I can use uses a different clone. Any thoughts on how to perform a good validation in this case? Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: IHC Validation
Thank you all for the info. I agree that in-house validation is the way to go. The only reason we had been using an outside reference lab was due to recommendations during a JC inspection which were applied to our area, but not really meant for IHC. The inspector was not a histology person, so it was a little difficult to get our point across. But they were adamant that with only one instrument the slides should be sent out for comparison. Thanks everyone! Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -Original Message- From: Morken, Timothy [mailto:timothy.mor...@ucsfmedctr.org] Sent: Friday, May 04, 2012 12:02 PM To: Dessoye, Michael J; histonet@lists.utsouthwestern.edu Subject: RE: IHC Validation All you need to do is compare the new clone to your current clone and show the new clone is concordant with the new clone for sensitivity and specificity . You don't need to compare to the same clone elsewhere. Tim Morken Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94132 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.mor...@ucsfmedctr.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J Sent: Friday, May 04, 2012 5:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Validation Hello all, A year or so ago, we upgraded from a Benchmark XT to Benchmark Ultra. For validation, we selected a variety of cases as usual and ran them on both instruments before we retired the XT. Now, when we add a brand new antibody, we again select a variety of cases, and once we're happy with them on the Ultra we send the same cases to a reference lab for comparison. I'm now faced with changing clones for several antibodies. I expected to go through pretty much the same validation procedure, but it got me thinking...the reference lab does not always use the same clone as some of ours. I suppose this really wouldn't be a 'true' validation in this case. Does anyone have any thoughts on this? The pathologists are perfectly happy with the staining of the new clone, but the only reference lab I can use uses a different clone. Any thoughts on how to perform a good validation in this case? Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Unregistered HT testing
Our interpretation of special stains/IHC/ISH is that it is the 'interpretation' of these stains that is 'high complexity'. I believe that CLIA is the place where 'high complexity' is defined (although CAP may expand on this) and CLIA does not recognize ASCP registration. Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -Original Message- From: Konop, Nicole [mailto:nko...@chw.org] Sent: Tuesday, April 17, 2012 3:37 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Unregistered HT testing Hello everyone! I'm just curious to know if anyone is allowing unregistered HT's to do special stains in their CAP accredited lab? I have been involved in discussions regarding high complexity testing. From the feedback I have received, special stains and IHC stains are considered high complexity testing. I beg to disagree. I can understand IHC/ISH as high complexity but I don't think routine special stains fall under that category. I'd appreciate any feedback or literature you can reference for me to review. Thank you! Nicole Anne Konop BS, HTL(ASCP) Histology Team Lead Children's Hospital of Wisconsin (414)266-6580 Direct Line (414)907-0366 Pager (414)266-2524 Histology Department _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Unregistered HT testing
Just to be clear, I wholly agree with your comments...I am not downplaying registration by any means, and I think that IHC and ISH should fall under high complexity. I think this issue gets downplayed because in many cases one can 'get by' by putting slides on a stainer and running it, and that doesn't 'appear' to be high complexity...but without the background knowledge and understanding that you talked about, these folks can't troubleshoot issues when they come up or understand the principles behind the procedures. To get back to the original question, I do know labs that have this testing performed by unregistered techs...however, they all have degrees and on-the-job training, so I imagine most would fall into the 'registration eligible' category, and be qualified for high-complexity testing. In my opinion, that would meet the CAP requirement. Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 From: joelle weaver [mailto:joellewea...@hotmail.com] Sent: Wednesday, April 18, 2012 1:17 PM To: Dessoye, Michael J; nko...@chw.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT testing CLIA does stipulate education requirements though for high complexity( I don't have it handy, but I do recall it is quite specific). Yes interpretation differs from performance. But we are not interpreting for diagnosis anyhow, but we do have to make technical assessments, corrections, and I think you need some insight to do that effectively. As I recall from CLIA, IHC/ISH is clearly under high complexity. But I can think of some histochemical staining ( such as enzyme muscle histochemistry) that might also be considered high complexity. I know for grossing, it is also an issue. Even if ASCP certification is not specifically stipulated in CLIA , I think that is is implied , in that the education stipulations are roughly equivalent to the criteria for HT certification- (associates with qtr. hours needed in science, chemistry etc). So yes I guess anyone can learn to put on and take off slides on a stainer and execute any process or procedure when trained to do so. The rub for me though is that I personally have observed that when people don't have training or education in histology ( formal or informal), they don't know how the stain works, or what it should show, so they are not adept at review of stained slides, and are not able to troubleshoot stain problems etc. I think that this should be part of the expectation, but that is an opinion, not I guess actually regulated as a requirement. I do think that CAP pretty much stipulates this expectation via competency requirements, but that is how I interpret it. Certification is just a means to communicate that you have satisfied the criteria, which should say that you have this underlying knowledge and understanding- knowing science just forms the basis for this understanding. I think good in house laboratory training and competency assessment could be an alternate route to that goal in places where more academic training opportunities are scarce. Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Wed, 18 Apr 2012 10:43:22 -0400 From: mjdess...@commonwealthhealth.net To: nko...@chw.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT testing CC: Our interpretation of special stains/IHC/ISH is that it is the 'interpretation' of these stains that is 'high complexity'. I believe that CLIA is the place where 'high complexity' is defined (although CAP may expand on this) and CLIA does not recognize ASCP registration. Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -Original Message- From: Konop, Nicole [mailto:nko...@chw.org] Sent: Tuesday, April 17, 2012 3:37 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Unregistered HT testing Hello everyone! I'm just curious to know if anyone is allowing unregistered HT's to do special stains in their CAP accredited lab? I have been involved in discussions regarding high complexity testing. From the feedback I have received, special stains and IHC stains are considered high complexity testing. I beg to disagree. I can understand IHC/ISH as high complexity but I don't think routine special stains fall under that category. I'd appreciate any feedback or literature you can reference for me to review. Thank you! Nicole Anne Konop BS, HTL(ASCP) Histology Team Lead Children's Hospital of Wisconsin (414)266-6580 Direct Line (414)907-0366 Pager (414)266-2524 Histology Department
[Histonet] CMV/HSV Protocols
Does anyone have protocols for CMV, HSV I, and HSV II (from Cell Marque) that they would be willing to share for the Benchmark Ultra (i-View)? Thanks! Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdess...@wvhcs.org mailto:mjdess...@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Slide/Block Retention
Hello Histonet, What policy is everyone following for slide and block retention? We are not CAP, however we currently keep slides and blocks for 10 years. My director wants to decrease that period to 2 years, which is the Joint Commission standard. I would like to keep 10 years because we frequently are asked to send slides for consult and review that are several years old. It is also helpful when looking for hard-to-find control tissue. However these reasons are apparently not sufficient. I'm leaning towards keeping the 10 year policy, but I need additional justification. Or does anyone think the 2 year period is sufficient? Thanks, Mike Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdess...@wvhcs.org mailto:mjdess...@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cell block HE staining
Hello all, I'm interested to see what staining protocol folks are using for HE staining of cell blocks. Lately we've been getting varying light and dark staining on cell blocks only. Currently they are run on our standard HE protocol that we use for all tissues. Does anyone use a separate protocol for cell blocks? Thanks! Mike Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdess...@wvhcs.org mailto:mjdess...@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] prostate bx containers
We still treat the specimens as though they were different specimen parts (A, B, C, D, etc...) I doubt that the physical arrangement of the containers affects the billing if you are still treating each core as a different specimen... From: Jones, Laura [mailto:lpjo...@srhs-pa.org] Sent: Mon 7/11/2011 1:15 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] prostate bx containers We looked at these, but our Pathologist's first question was can we bill for 6 or 8 bottles if they are technically all in one? How is that handled? From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J [mjdess...@wvhcs.org] Sent: Monday, July 11, 2011 11:44 AM To: Sheila Adey; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] prostate bx containers We don't currently use them but I've used them in the past. As long as you don't 'bump' them on the grossing station while they're open, they're fine. I have yet to see one leak. Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdess...@wvhcs.org mailto:mjdess...@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 From: Sheila Adey [mailto:sa...@hotmail.ca] Sent: Sat 7/9/2011 9:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] prostate bx containers Hello: Has anyone used these prostate biopsy collection containers from Simport? It looks like a petri dish and has 12 individual wells in it, each one labelled with the location from the prostate. We are going to trial them but I would like some opinions on them. We currently get 10 to 12 bottles per case. So, all the bx's could go in this one container. Thanks Sheila _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com http://www.sharonregional.com/ for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] prostate bx containers
We don't currently use them but I've used them in the past. As long as you don't 'bump' them on the grossing station while they're open, they're fine. I have yet to see one leak. Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdess...@wvhcs.org mailto:mjdess...@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 From: Sheila Adey [mailto:sa...@hotmail.ca] Sent: Sat 7/9/2011 9:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] prostate bx containers Hello: Has anyone used these prostate biopsy collection containers from Simport? It looks like a petri dish and has 12 individual wells in it, each one labelled with the location from the prostate. We are going to trial them but I would like some opinions on them. We currently get 10 to 12 bottles per case. So, all the bx's could go in this one container. Thanks Sheila _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HistoGel
Hello, Does anyone out there have any experience with HistoGel? It's Richard Allan/Thermo Fisher. They claim that you can embed scant tissues in the gel and then process, embed, and cut as usual. Just wondering how it works in the real world Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdess...@wvhcs.org mailto:mjdess...@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Keyboard and mouse covers
Hi, We're upgrading our grossing areas with new workstations that require the techs to interact with a keyboard and mouse. I'd like to get keyboard and mouse covers to protect them from the usual hazards of grossing! But I can't seem to find any big vendors that sell them...does anyone use them and could recommend a vendor for these covers? Thanks! Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdess...@wvhcs.org mailto:mjdess...@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] p63
Biocare has a very good, and fairly inexpensive, p63 that we have had good results with: http://biocare.net/products/antibodies/p/163/ From: Joanne Clark [mailto:jcl...@pcnm.com] Sent: Mon 5/23/2011 12:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p63 I need to find a source for p63 antibody. Where does everyone purchase theirs from? Joanne Clark,HT Pathology Consultants of New Mexico _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Benchmark Problems
Thank you everyone for the helpful replies and info. I followed everyone's suggestions while waiting for service and didn't see any significant improvement. Once the service tech took everything apart he found the nozzle that controls dispensing of the bulk reagents was partially clogged causing the bulk reagents to be dispensed unevenly (usually totally off the slide!) Things straightened out afterwards. Thanks again everyone! Mike Dessoye From: Mark Tarango [mailto:marktara...@gmail.com] Sent: Wed 2/9/2011 11:58 AM To: Dessoye, Michael J Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Benchmark Problems Hi Mike, Did you wash the bottles and rinse them and then pump the water through the instrument before adding reagents and purging all again? We had a problem like this about 2 weeks ago. I think someone loaded the wrong bulk reagent onto the instrument. Cleaning it, purging with water and adding newly made bulk reagents fixed the problem for us. Mark On Wed, Feb 9, 2011 at 8:52 AM, Dessoye, Michael J mjdess...@wvhcs.org wrote: Hello, We started having a strange problem with our Ventana Benchmark XT. Out of the blue, our slides stopped staining. With most antibodies we get no reaction, some that were very strongly positive are now only very lightly staining. They do appear to counterstain OK. Following Ventana's recommendations we completely changed out all of our bulk fluids, purged the lines, changed antibodies and DAB kits. The last shot is some kind of instrument issue that's not triggering an error. Anyone ever see anything like this? Mike Dessoye _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Benchmark Problems
Hello, We started having a strange problem with our Ventana Benchmark XT. Out of the blue, our slides stopped staining. With most antibodies we get no reaction, some that were very strongly positive are now only very lightly staining. They do appear to counterstain OK. Following Ventana's recommendations we completely changed out all of our bulk fluids, purged the lines, changed antibodies and DAB kits. The last shot is some kind of instrument issue that's not triggering an error. Anyone ever see anything like this? Mike Dessoye _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Pax-2
Pax-2 (rabbit poly) is available from Cell Marque but in our experience it also has some cytoplasmic background staining. Mike Dessoye --- Message: 21 Date: Tue, 4 Jan 2011 16:06:56 -0800 From: Mark Tarango marktara...@gmail.com Subject: [Histonet] Pax-2 To: histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu Message-ID: aanlktimha5h26-8o6fx4wuk5oplobrog4nexmpsnt...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Hi Histonet, My lab needs to switch vendors on this antibody. Where is everyone buying Pax-2? I found on their website that Invitrogen sells an IVD rabbit poly Pax-2 antibody. Has anyone tried this or others? What about Epitomics RUO rabbit mono? Do all the antibodies give that cytoplasmic background staining that I've seen? I'd appreciate any info at all. Thanks Mark Tarango _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Lab Chairs
We have office and lab chairs from Haworth. They're very expensive, with ours around $800, but they have a lifetime warranty. We just had two sent for repair or replacement that were 10+ years old and there was no problem. Mike Dessoye -- Message: 1 Date: Wed, 5 Jan 2011 11:17:26 -0600 From: Jay Lundgren jaylundg...@gmail.com Subject: Re: [Histonet] Lab chairs To: Liz Chlipala l...@premierlab.com Cc: histonet@lists.utsouthwestern.edu, Sharon.Davis-Devine sharon.davis-dev...@carle.com Message-ID: aanlktin1dkhto+o0guy-cexiq2l8k43jnc9puo98k...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Good luck finding good task chairs that don't cost a fortune. The good ones are all very expensive now days. I think that buying cheap ones is a false savings, however, as they will fall apart in short order. I have personally seen a busy histo lab destroy new (cheap) chairs in a year. Sincerely, Jay A. Lundgren M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 2 Date: Wed, 5 Jan 2011 09:26:46 -0800 (PST) From: Sheila Haas micropathl...@yahoo.com Subject: Re: [Histonet] Lab chairs To: Jay Lundgren jaylundg...@gmail.com, Liz Chlipala l...@premierlab.com Cc: histonet@lists.utsouthwestern.edu, Sharon.Davis-Devine sharon.davis-dev...@carle.com Message-ID: 967402.42392...@web57806.mail.re3.yahoo.com Content-Type: text/plain; charset=iso-8859-1 I have to agree with Jay. A couple of years ago, we purchased new, expensive lab chairs (from an office supply store that customized) and they look great even now. I think it's worth the money from an ergonomic standpoint and also because they hold up much better. Just my 2 cents! Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. From: Jay Lundgren jaylundg...@gmail.com To: Liz Chlipala l...@premierlab.com Cc: histonet@lists.utsouthwestern.edu; Sharon.Davis-Devine sharon.davis-dev...@carle.com Sent: Wed, January 5, 2011 12:17:26 PM Subject: Re: [Histonet] Lab chairs Good luck finding good task chairs that don't cost a fortune. The good ones are all very expensive now days. I think that buying cheap ones is a false savings, however, as they will fall apart in short order. I have personally seen a busy histo lab destroy new (cheap) chairs in a year. Sincerely, Jay A. Lundgren M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 3 Date: Wed, 5 Jan 2011 12:49:54 -0500 From: Weems, Joyce jwe...@sjha.org Subject: RE: [Histonet] inventory To: Kelly Boyd kdboydhi...@yahoo.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 92ad9b20a6c38c4587a9febe3a30e1640814f91...@chexcms10.one.ads.che.org Content-Type: text/plain; charset=iso-8859-1 We do it annually. We use a combination of reports from our Materials Management Dept. and from our order spread sheet. (I keep a spread sheet of all items ordered). It's not totally accurate because of the chemicals and stains that have been here forever, but it's close enough for Mat Mgt. Best, Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kelly Boyd Sent: Wednesday, January 05, 2011 12:08 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] inventory Yesterday I inquired If and how labs did inventory for specials and immunos. I meant to ask IF and HOW labs do end of year inventory for specials and immunos. Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic
