Re: [Histonet] Leica CM3050S available

[Garcia, Lori, M.Sc. via Histonet]

Thanks to everyone for responding to the below offer. I tried to respond to 
each individual email, but just in case, we have promised the cryostat to 
someone for educational purposes.

Regards,
Lori Garcia

From: Garcia, Lori, M.Sc.
Sent: Friday, March 23, 2018 6:26 PM
To: histonet@lists.utsouthwestern.edu
Subject: Leica CM3050S available

Hi everyone,

After building and running a histology lab for over 13 years, my company has 
decided to shut down internal processing and convert the space. I have a Leica 
CM3050S cryostat that is in pristine condition and would be happy to donate to 
someone in need. The only cost will be the shipping charges. I can also send 
all accessories that we have.

Thanks,
Lori Garcia
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Re: [Histonet] [EXTERNAL] Deplastifying MMA

[Garcia, Lori, M.Sc. via Histonet]

Hi Reuel,

One thing you could try is THF (tetrahydrofuran). We have used it in the past 
to rapidly dissolve the MMA on slides.

Good luck,
Lori

-Original Message-
From: Dorothy Hu via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, February 14, 2018 11:46 AM
To: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] [Histonet] Deplastifying MMA

>
> Hi  Reuel
>

We had the same thing as you. It was caused by bad MMA, I tested. Write down 
the lot # and ask refund. Test new lot # 1st of MMA in the future. It was very 
frustratingI know.
Best luck.

Dorothy





> Message: 2
> Date: Mon, 12 Feb 2018 21:07:15 +
> From: Reuel Cornelia 
> To: "histonet@lists.utsouthwestern.edu"
> 
> Subject: [Histonet] Deplastifying MMA
> Message-ID:
>  namprd17.prod.outlook.com>
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hello histonetters,
>
> I have a problem with my MMA plastic section on my slide. I could not
> remove the plastic MMA even if I leave the section slides in Xylene
> for several days with heat incubation at 60 degrees. This is the first
> time that happened to my section for several years of doing the same thing 
> over.
> I was thinking that it was my Xylene lot number so I tried to change
> it but still does not remove the plastic, and then Itried different
> solvent Acetone, and Ethylene glycol Monoethyl ether but still it does not 
> work.
> Can I ask for help if anyone knows what was going on and what would be
> the best way to remove this plastic from my section?
>
> Just to give you my embedding solution was MMA -94% , dibutly
> phthalate- 5% and perkadox 16- 0.5%. This have been my solution for
> years and I do not have any problem with the removal of plastic
> section until now. I was thinking that my MMA (M55909) different lot
> number from Sigma Aldrich may have cause this because even I tried to
> use the MMA to dissolve my plastic it does not work.
>
>
> Thank you for and any opinions or protocols are greatly appreciated.
>
>
> Reuel Cornelia
>
> TSRH
>
> 214-559-7766
>
>
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Re: [Histonet] A Question About Paraffin Times

[Garcia, Lori, M.Sc. via Histonet]

Hi Paula,

I am in agreement with everyone else about putting the processor on a delay so 
it will finish when needed. However, if that is not possible, it is perfectly 
fine for the blocks to harden at room temp, not freezing, and then be warmed up 
again for embedding. This has come up in my research lab, and we haven't had 
any problems.

Regards,
Lori

This message has been marked as Medtronic Controlled
-Original Message-
From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, May 16, 2017 11:30 AM
To: Logan, Shannon 
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] A Question About Paraffin Times

Hi Shannon and Everyone Else,

Usually the samples are placed on a weekend delay.  This time, however, the 
breast cases were sitting in formalin since Thursday and the run had to end on 
Saturday due to the CAP 72 hour rule for breast and HER2 testing.

The problem is, the person in charge of Histology not a Histologist.  The 
person in charge was told by several experienced histotechs that letting the 
samples sit in molten paraffin is not a good thing to do.  They were told that 
it was routine (which it isn't) to let them sit in hot wax for days.

I just need information that states that sitting in paraffin for any excessive 
length of time is bad.  I found it mentioned in Sheehan and Hrapcek's "Theory 
and Practice of Histotechnology".

I can't find my Carson.  Does she mention excessive times in paraffin?

If so, please let me know.

Thank you for all the assistance.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872 <#>



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On Tue, May 16, 2017 at 11:13 AM, Logan, Shannon 
wrote:

> Hello Paula,
>
> So why must the cassettes be removed on Saturday morning if you aren’t
> embedding until Monday morning?
>
> Don’t you have a “delay start” feature on your processor? Neither
> option A or B seem like a good thing for the tissue!
>
>  We time our processor to finish at 5 AM Monday when the first
> Histotech arrives for embedding.
>
> The cassettes remain in formalin until the processor starts up on
> Sunday evening.
>
>
>
> Regards,
>
>
>
>
>
> Shannon H. Logan  B.S., HTL (ASCP)
>
> Pathology Department
>
>
>
> Bellin Health Memorial Hospital
>
> 744 South Webster Avenue
>
> Green Bay, WI 54305-3400
>
> 920-433-3653  X3727 <(920)%20433-3653>
>
>
>
>
>
>
>
> *From:* P Sicurello via Histonet
> [mailto:histonet@lists.utsouthwestern.edu]
>
> *Sent:* Tuesday, May 16, 2017 10:34 AM
> *To:* HistoNet
> *Subject:* [Histonet] A Question About Paraffin Times
>
>
>
> Good Morning Listers,
>
> I am asking the collective wisdom of the Histonet this question:
>
>
> Is it better to remove baskets from the processor on Saturday morning and:
>
> A. Let the cassettes freeze, then melt them down and embed Monday morning?
> OR
> B. Leave the cassettes in molten paraffin and embed Monday morning?
>
>
> I am of the opinion that leaving the samples (not fatty, like breast
> cores) in molten paraffin (62 degrees C) is bad practice, and causes
> them to get "crunchy", among other things.
>
> What do you think?
>
> Thank in advance.
>
> Sincerely,
>
> Paula Sicurello, HTL (ASCP)CM
>
> Histotechnology Specialist
>
> UC San Diego Health
>
> 200 Arbor Drive
>
> San Diego, CA 92103
>
> (P): 619-543-2872 <(619)%20543-2872> <#>
>
>
>
> *Confidentiality Notice*: The information transmitted in this e-mail
> is intended only for the person or entity to which it is addressed and
> may contain confidential and/or privileged material. Any review,
> retransmission, dissemination or other use of or taking of any action
> in reliance upon this information by persons or entities other than
> the intended recipient is prohibited. If you received this e-mail in
> error, please contact the sender and delete the material from any computer.
> ___
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[Histonet] EXAKT band saw blade

[Garcia, Lori, M.Sc. via Histonet]

Looking for an EXAKT band saw blade for a colleague due to a backorder. If 
anyone has one to loan out or sell, please contact me.

Thanks,
Lori
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