Re: [Histonet] inverted meniscus
Maybe it twisted it's knee while out playing touch football. From: Mary Benoit mbp...@yahoo.com To: histonet@lists.utsouthwestern.edu Date: 08/11/2010 01:21 PM Subject: [Histonet] inverted meniscus Sent by: histonet-boun...@lists.utsouthwestern.edu Today while perfroming an xylene purity check after recyling xylene on a CBG recycler, I noticed that the meniscus between the DH2O and Xylene is inverted...that is convex instead of concave. I have done this check many times( you add exactly 15 mls of water to exactly 85 mls xylene, invert and allow to settle out and observe the meniscus at the separation site for changes in volume of water) and have never seen it invert. The glass cyclinder was clean , we repeated three times and still same results. Anyone have a clue as to why? CBG did not have an answer . thanks Mary F Benoit MT(ASCP) The Pathology Laboratory Lake Charles, LA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Sakura Tape coverslipper
OMG. This problem was ever present in our lab for years, until I figured out that ANY minute trace of moisture, water, eosin in the last absolute alcohol would interfere with the activation of the adhesive in the tape, and create this brown artefact. We religiously change our absolute alcohols on the autostainer so the last alcohol is perfectly clear of any hint of eosin, and the artefact has disappeared from our tape-coverslipped slides.The Sakura rep couldn't help me either, and told me they had NEVER seen this problem before. Love it. Keep your last 100% PRISTINE, and the artefact will be eliminated. I promise. Jackie O' From: Nails, Felton flna...@texaschildrens.org To: 'Gauch, Vicki' gau...@mail.amc.edu, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: 08/11/2010 01:45 PM Subject: [Histonet] Sakura Tape coverslipper Sent by: histonet-boun...@lists.utsouthwestern.edu Has anyone been experiencing a brown cornflaking artifact on their slides after being coverslipped. I was told by Sakura that it was do to the slides drying out when transferred from the stainer to the coverslipper. Which can't be correct because on the new sakura coverslipper there is a holding area which is filled with xylene so if the tissue dried out it would rehydrate before being coverslipped. - CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Reagent Grade Alcohol Brands
What kind of issues? I'm considering switching over to all Surgipath (Leica) products. I'd like to hear more of this. From: kristen arvidson arvidsonkris...@yahoo.com To: histonet histonet@lists.utsouthwestern.edu Date: 08/09/2010 12:05 PM Subject: [Histonet] Reagent Grade Alcohol Brands Sent by: histonet-boun...@lists.utsouthwestern.edu Hello All, What brands of reagent grade ETOH is everyone using?? Leica is having some manufacturing issues...need some ASAP!! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Elgin Community College Histology Program
Does anyone know who is running this program? I would like to contact them. Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] (no subject)
Ask management if he would suddenly be a better technician and more valuable if he passed the exam? Duh - no. In my opinion, merit increases should be based on merit - not documentation. If he's not eligible, he's not eligible. Does your management increase pay based on certification? So, if a certified tech with one year of experience came to your lab, they would be paid more than an incumbent tech with 8 years experience? Sounds like you have some dinosaur management there. From: Fredrickson, Mona mfredrick...@nrh-ok.com To: Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu Date: 07/07/2010 12:04 PM Subject: [Histonet] (no subject) Sent by: histonet-boun...@lists.utsouthwestern.edu Hello All in Histoland, I have a tech who is employed as Histotech eligible, but he was not able to pass the HT exam and now is no longer eligible to thake the test because he has to get his associates degree in science. The lab management wants to change his title from histotech eligible to histotech non-registered without pay increase. But I feel the pay should be increased. So I would appreciate comments on the following: 1.) should title be changed from eligible to non-registered? 2.) After having done this for 8 years should his pay stay the same? 3. Should job responsibilities remain the same Thank you in advance for feedback! Histotech in Oklahoma FONT face=Tahoma size=2 FONT color=#ffUFONT size=2 PCONFIDENTIALITY NOTICE:/U/P PThis e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments./FONT/FONT/FONT/P ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (no subject)
Over the years, I have had some HT and HTL registered technologists who were crummy technicians. I'm jus' sayin' that certification doesn't automatically mean you are better than a non-certified tech. From: Gunderson, Michael mgund...@fairview.org To: Jackie M O'Connor Jackie.O'con...@abbott.com, Fredrickson, Mona mfredrick...@nrh-ok.com Cc: Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu, histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu Date: 07/07/2010 12:31 PM Subject: RE: [Histonet] (no subject) By telling someone they should earn the same without passing our registration exam diminishes the effort and hard work of registered HT and HTL's. Management are not dinosaurs, they are fair. The exam is there to weed out the weak and under or un-qualified, if you cannot pass the test, you have not earned the right of those who have. Michael A. Gunderson HTL(ASCP) Lead Technologist-Immunostains Laboratory University of Minnesota Medical Center-Fairview 2450 Riverside Avenue Minneapolis, MN 55454 Laboratory: 1-612-273-9119 Fax: 1-612-273-4879 Email: mgund...@fairview.org From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor [Jackie.O'con...@abbott.com] Sent: Wednesday, July 07, 2010 12:09 PM To: Fredrickson, Mona Cc: Histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] (no subject) Ask management if he would suddenly be a better technician and more valuable if he passed the exam? Duh - no. In my opinion, merit increases should be based on merit - not documentation. If he's not eligible, he's not eligible. Does your management increase pay based on certification? So, if a certified tech with one year of experience came to your lab, they would be paid more than an incumbent tech with 8 years experience? Sounds like you have some dinosaur management there. From: Fredrickson, Mona mfredrick...@nrh-ok.com To: Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu Date: 07/07/2010 12:04 PM Subject: [Histonet] (no subject) Sent by: histonet-boun...@lists.utsouthwestern.edu Hello All in Histoland, I have a tech who is employed as Histotech eligible, but he was not able to pass the HT exam and now is no longer eligible to thake the test because he has to get his associates degree in science. The lab management wants to change his title from histotech eligible to histotech non-registered without pay increase. But I feel the pay should be increased. So I would appreciate comments on the following: 1.) should title be changed from eligible to non-registered? 2.) After having done this for 8 years should his pay stay the same? 3. Should job responsibilities remain the same Thank you in advance for feedback! Histotech in Oklahoma FONT face=Tahoma size=2 FONT color=#ffUFONT size=2 PCONFIDENTIALITY NOTICE:/U/P PThis e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments./FONT/FONT/FONT/P ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] ARTICLE
No - but I could write one. From: Sara Baldwin/mhhcc.org sbald...@mhhcc.org To: histonet@lists.utsouthwestern.edu Date: 06/25/2010 10:53 AM Subject: [Histonet] ARTICLE Sent by: histonet-boun...@lists.utsouthwestern.edu Hi Histonetters My boss was wondering if anyone has come across an article a long time ago (about 10 years) that was called Workplace Violence in the Laboratory Thanks Pathology Supervisor Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbald...@mhhcc.org Ph 812-482-0210, 482-0216, Fax 812-482-0232, Pager 812-481-0897 Confidential information, Authorized use only. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Help! In need of positive Gram Control
A good ol' hot appendix works great. Not as good as a Slim Jim, tho. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] afb contamination
You can eliminate the crossover contamination by performing the stain horizontally on a staining rack. A lot of labs re-use the carbol fuchsin, which can have lots of extraneous AFB floating around, waiting patiently to attach itself to an unsuspecting patient slide. Using the staining rack, you don't use a lot of reagent, and you never cross-contaminate. From: Tench, Bill bill.te...@pph.org To: histonet@lists.utsouthwestern.edu Date: 05/21/2010 02:34 PM Subject: [Histonet] afb contamination Sent by: histonet-boun...@lists.utsouthwestern.edu We had a problem with contamination on our AFB stains, and we discovered that it was the control slide flaking off in the copland jar which was being used for staining the control and target slide at the same time (makes sense as a real control'). We identified these contaminants because they were frequently large clusters (by large I would say 4-8 organisms) which we almost never see in real cases, and fortunately, they were also not in the same plane of focus (but that can be subtle). they did create problems. Our solution was to stain the control separately from the case. No more problems. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 mail2.pph.org made the following annotations - Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] alternative for bunsen burner?
I think that's the crux of her question - what can she use as an alternative? You can find forceps warmers here. http://americanmastertech.com/store/main.aspx?p=ItemDetailStylesitem=EQFW-120 Although, I still miss the ol' bunsen burner. It was great for burning away little fragments of tissue from between the teeth - of the forceps, not my teeth. From: Mike Pence mpe...@grhs.net To: Brandi Higgins brandihigg...@gmail.com, histonet@lists.utsouthwestern.edu Date: 05/18/2010 11:42 AM Subject: RE: [Histonet] alternative for bunsen burner? Sent by: histonet-boun...@lists.utsouthwestern.edu Why are you still using a bunsen burner? I believe there is a CAP question about No open flames in the lab. I would suggest finding a replacement. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Brandi Higgins Sent: Tuesday, May 18, 2010 10:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] alternative for bunsen burner? Hello all, Our hospital is moving our histology department across the hall and have asked us if we can find an alternative to the bunsen burner so they don't have to install a gas line. The pathologist has told me that there is some machine that can be used instead of a flame to burn the forceps while embedding (which is our main use for the bunsen flame). This is the only lab I have ever worked at and I don't know what such a machine would be called. If anyone knows the name, or any other alternative method can you let me know. Model numbers/companies would be a bonus too! Thanks so much for your help. Brandi Higgins, BS, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FREE MONEY!
Now that I have your attention - - I would really like to hear from you if you are at all interested in a histology position at Abbott Laboratories in Northern Illinois. Even if you have applied previously. This is a great opportunity to work in a GLP research lab, a good deal of bench work, but developing IHC for toxicology purposes as well. We have a Biocare intelliPATH stainer - it's FUN! We have four full time technicians/technologists with a fifth open position. Abbott offers 3 weeks paid vacation, great benefits, and is a good company to work for. I've been here ten years, and I've never been anywhere 10 years. Please forward your resume to me, as well as go to www.Abbott.com to apply. Jackie O'Connor ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Job Opportunity
We have a great full time permanent opportunity for an experienced histotech at Abbott Laboratories in Abbott Park, IL. We are about 40 miles north of Chicago in Lake County. Please go to www.Abbott.com if you are interested in applying for this position. Additionally, if you would like to forward your resume to me, it could be helpful. Jackie O' ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] lab spill clean-up
I've always been in institutions (hey, no jokes) where more than a liter of spilled formalin, alcohol, xylene, etc is cleaned up by the in house haz mat people. From: kristen arvidson arvidsonkris...@yahoo.com To: histonet histonet@lists.utsouthwestern.edu Date: 11/05/2009 02:22 PM Subject: [Histonet] lab spill clean-up Sent by: histonet-boun...@lists.utsouthwestern.edu I am looking for quantities of various lab chemicals that are considered safe for lab personnel to clean up. Are there set guidelines for when you should call for help? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Looking for Ray Ortiz
Can someone tell Ray to contact me? Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Arm Rests
Yeah - don't use em. Since I had developed tendinitis in both elbows from microtomy, EHS ergonomics people at a former employer decided to invent some. They covered them with some fake vinyl material, and the very first day I tried using them, my elbow slipped on the shiny vinyl and I cut off the tip of my finger on the blade. I have since learned that resting your forearms on the sharp edge of the bench caused my tendinitis. Since I quit that bad habit, I haven't had a recurrence in 15 years - nor have I cut myself. From: Weems, Joyce jwe...@sjha.org To: histonet@lists.utsouthwestern.edu Date: 11/03/2009 12:58 PM Subject: [Histonet] Arm Rests Sent by: histonet-boun...@lists.utsouthwestern.edu Does anyone have a recommendation for microtome arm rests? Thanks in advance! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Canine endothelial antibody
A week or so ago I thought I saw a post about an antibody that works for canine endothelial cells, but I can't find it in the archives - was I just dreaming, because I need this so bad? I've tried Factor VIII and a CD34 on FFPE, but with poor results. Any help would be most appreciated. Thanks, Jackie O' ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Floaters in Waterbath
Kim wipes seem to pick up more debris than paper towels, and they pick up much less water. We routinely sweep the waterbath with a kimwipe after each block. You can also pick up floaters from embedding if the forceps are not cleaned between each block. Most embedding centers have multiple wells for forceps - how often do you clean those wells? You'd be amazed at how much gunk accumulates in there! From: Stella Mireles estellamire...@gmail.com To: Histonet@lists.utsouthwestern.edu Date: 10/23/2009 09:11 AM Subject: [Histonet] Floaters in Waterbath Sent by: histonet-boun...@lists.utsouthwestern.edu I know we have all had some problems with floaters in our waterbath at some point in our microtomy career. Our doctors are very picky and I need some tips on keeping an immaculate clean waterbath, but not sacrificing the speed in a regular routine lab. We use the pyrex waterbath and paper towels for wiping our area. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] human macrophages in human melanoma xenografts
Naira - These are presumably SCID mice? You can use a mouse mAb with no Ig interference, since SCIDs do not have an immune system. You can get the rare 'leaker', but I've used murine mAb routinely for human xenografts in SCIDs. Now, Nudes are a different story. From: Margaryan, Naira nmargar...@childrensmemorial.org To: Liz Chlipala l...@premierlab.com, Galbraith, Joe joseph-galbra...@uiowa.edu, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: 10/23/2009 10:59 AM Subject: RE: [Histonet] human macrophages in human melanoma xenografts Sent by: histonet-boun...@lists.utsouthwestern.edu Thanks a lot both of you! For melanoma detection, I usually use HRP with AEC. Is there any non-mouse anti-human macrophage marker? Thank you much, Naira -Original Message- From: Liz Chlipala [mailto:l...@premierlab.com] Sent: Friday, October 23, 2009 10:51 AM To: Galbraith, Joe; Margaryan, Naira; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts F4/80 is a mouse macrophage marker. If she wants to detect human macrophages in a mouse background she will need to use a mouse anti-human CD68. She will need to run it with a mouse on mouse detection system and run all of the appropriate negative controls. I would also select a alkaline phosphatase detection system rather than HRP with DAB just incase the tumor has any melanin in it. I have done this before looking for human lymphocytes with LCA in a mouse background. You just need to make sure you have all of the appropriate controls. For a positive control I would use a human tonsil or something like that and you need to make sure you run the appropriate isotype negative controls. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Galbraith, Joe Sent: Friday, October 23, 2009 9:44 AM To: Margaryan, Naira; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] human macrophages in human melanoma xenografts Naira: Here is a link to a site listing macrophage markers. F4/80 is a commonly mentioned marker for macrophages. I presume you mean IHC rather than ICH. Enjoy. http://www.antibodybeyond.com/reviews/cell-markers/macrophage-marker.htm Joe joseph-galbra...@uiowa.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Friday, October 23, 2009 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] human macrophages in human melanoma xenografts Hi Histonetters, I would like to be able to look at human macrophages in human melanoma xenografts raised in mouse. Could you please suggest me a best Ab for ICH and protocol? Thanks in advance, Naira ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Asbestos Body Digestion
When I was in Honolulu, loaded with shipyards - we frequently digested lung tissue in sodium hypochlorite (bleach) to get an aggregate of asbestos fibers (if they were present). I don't recall the exact procedure, but it was quite common then. We were requested to do this by lawyers, mostly. So, I'm really not much help, just full of useless rhetoric. Hugh Luk might remember doing this? From: Weems, Joyce jwe...@sjha.org To: histonet@lists.utsouthwestern.edu Date: 09/22/2009 01:29 PM Subject: RE: [Histonet] RE: Asbestos Body Digestion Sent by: histonet-boun...@lists.utsouthwestern.edu Just trying to help a patient. I have a patient who wants to know if her lung adenoca could be tested to see if any asbestos could be involved. One of my pathologists suggested I call Mayo. Nothing there. He said someone used to do the digestion procedure at SUNY. So I asked the experts. I have a procedure from Lynette but I think I need an expert to do the testing and the resulting. Joyce -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: Tuesday, September 22, 2009 14:15 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Asbestos Body Digestion Joyce Why do you want to dissolve these? I seem to remember that these are usually characteristic shaped structures that are iron positive and should be easy to identify on morphology alone or am I mistaken? Barry -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Tuesday, September 22, 2009 10:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Asbestos Body Digestion Does anyone know about a digestion procedure for asbestos bodies on FFPE lung tissue? Our pathologist remembers that someone used to do this at SUNY. I'd appreciate your feedback. Thanks, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] mice legs
I did a lot of IHC work with whole mouse legs where we had to pay close attention to the tibia, as well as the joint.When the mice were necropsied, I had the prosectors remove all the soft tissue and foot without disrupting the joint. They could do this easily with fresh tissue as opposed to after fixation. The leg bones were fixed for no more than 48 hours, and decalcified in home-brewed 5% formic acid overnight on a shaker table. I then trimmed them to expose the joint and bone marrow prior to processing on a routine program, about 45 minutes per station. Perfect ever single time. IHC was beautiful. Hope this helps. Jackie O' From: Shaw, Sharon shs...@wpi.edu To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: 09/15/2009 08:46 AM Subject: [Histonet] mice legs Sent by: histonet-boun...@lists.utsouthwestern.edu Good Morning Histo World, I would like to know if anyone is working with mice legs, I have a PI that I work with that wants to process the whole leg, the problem is I need to decal it first and is wondering if the decal will break down the tissue, I think it would he doesn't think so. And if anyone has do this would it be possible to share your protocol with me from decal to processing. Thanks, Sharon- WPI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] formalin storage
I once had my safety officer insist I wear chain maille gloves while cutting frozen sections. They didn' t care about all the reasons I gave them why I shouldn't - like it would be impossible to use the machine while wearing them, and the patient would have to lie on the operating table longer waiting to find out if their entire colon was going to be removed. Jean Warren jwarre...@cinci.rr.com Sent by: histonet-boun...@lists.utsouthwestern.edu 09/11/2009 08:25 AM To Riesen, Rebecca rebecca.rie...@nchmd.org, histonet@lists.utsouthwestern.edu cc Subject Re: [Histonet] formalin storage No, it is ridiculous. Safety people tried to argue this with us years ago. One of our pathologists told them, How can something that is almost 90% water be a combustion hazard? - Original Message - From: Riesen, Rebecca rebecca.rie...@nchmd.org To: histonet@lists.utsouthwestern.edu Sent: Friday, September 11, 2009 9:15 AM Subject: [Histonet] formalin storage We have been directed by our Safety Officer to store all formalin (37% and 10% NBF) in a flammable storage room, cabinet or container. Yes, 37% Formalin we do store in this manner, but I have never heard of this requirement for 10%NBF. I looked on line to many MSDS sheets from different vendors and found only one that stated such storage requirements for 10% NBF. During this search I found all but one company states that formalin is not flammable. I brought this to the Safety Officer. He agrees that it is not flammable but that it IS combustible. Combustible=Flash point of 100F to 200F. Of the dozen sites I visited I found the following data concerning the Flash Point of 10% NBF: from NA / 200F / 122F to 185F. The NFPA (National Fire Protection Agency) guideline of no more than 1 gallon in a flammable storage container and 1 gallon outside of a safety cabinet/container per 100 square feet is already quite limiting. Using this guideline, we have calculated acceptable volumes of the known flammables (Alcohols and Xylenes) we can store. Adding 10% NBF to the equation will have us traveling to our bulk storage area constantly. Does anyone out there store 10%NBF in flammable cans/cabinets? Riesen, Rebecca rebecca.rie...@nchmd.org NCH Healthcare Systems Direct 239-436-5000 x2188 Fax 239-436-6767 Visit our website at http://www.nchmd.org CONFIDENTIALITY NOTICE This email and any files transmitted with it are from the NCH Healthcare System. This message is confidential and is intended only for the addressee. If you are not the intended recipient or have received this email in error, please call us immediately at (239) 436-5000 and ask to speak to the message sender or promptly email the message sender of the delivery error and then delete the message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] formalin storage
To prevent getting cut on the knife. Emily Sours talulahg...@gmail.com 09/11/2009 08:34 AM To Jackie M O'Connor Jackie.O'con...@abbott.com, histonet@lists.utsouthwestern.edu cc Subject Re: [Histonet] formalin storage I have to ask--what was the point of chain mail gloves?! Emily One of the defining characteristics of modern surgery was that patients ought to survive it. --Peter Stanley, For Fear of Pain: British Surgery, 1790-1850 On Fri, Sep 11, 2009 at 9:31 AM, Jackie M O'Connor Jackie.O'con...@abbott.com wrote: I once had my safety officer insist I wear chain maille gloves while cutting frozen sections. They didn' t care about all the reasons I gave them why I shouldn't - like it would be impossible to use the machine while wearing them, and the patient would have to lie on the operating table longer waiting to find out if their entire colon was going to be removed. Jean Warren jwarre...@cinci.rr.com Sent by: histonet-boun...@lists.utsouthwestern.edu 09/11/2009 08:25 AM To Riesen, Rebecca rebecca.rie...@nchmd.org, histonet@lists.utsouthwestern.edu cc Subject Re: [Histonet] formalin storage No, it is ridiculous. Safety people tried to argue this with us years ago. One of our pathologists told them, How can something that is almost 90% water be a combustion hazard? - Original Message - From: Riesen, Rebecca rebecca.rie...@nchmd.org To: histonet@lists.utsouthwestern.edu Sent: Friday, September 11, 2009 9:15 AM Subject: [Histonet] formalin storage We have been directed by our Safety Officer to store all formalin (37% and 10% NBF) in a flammable storage room, cabinet or container. Yes, 37% Formalin we do store in this manner, but I have never heard of this requirement for 10%NBF. I looked on line to many MSDS sheets from different vendors and found only one that stated such storage requirements for 10% NBF. During this search I found all but one company states that formalin is not flammable. I brought this to the Safety Officer. He agrees that it is not flammable but that it IS combustible. Combustible=Flash point of 100F to 200F. Of the dozen sites I visited I found the following data concerning the Flash Point of 10% NBF: from NA / 200F / 122F to 185F. The NFPA (National Fire Protection Agency) guideline of no more than 1 gallon in a flammable storage container and 1 gallon outside of a safety cabinet/container per 100 square feet is already quite limiting. Using this guideline, we have calculated acceptable volumes of the known flammables (Alcohols and Xylenes) we can store. Adding 10% NBF to the equation will have us traveling to our bulk storage area constantly. Does anyone out there store 10%NBF in flammable cans/cabinets? Riesen, Rebecca rebecca.rie...@nchmd.org NCH Healthcare Systems Direct 239-436-5000 x2188 Fax 239-436-6767 Visit our website at http://www.nchmd.org CONFIDENTIALITY NOTICE This email and any files transmitted with it are from the NCH Healthcare System. This message is confidential and is intended only for the addressee. If you are not the intended recipient or have received this email in error, please call us immediately at (239) 436-5000 and ask to speak to the message sender or promptly email the message sender of the delivery error and then delete the message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Decal Solution
I have decalled whole mouse legs (soft tissue removed). Use 5% formic acid for 24 hours on a shaker table. Shaw, Sharon shs...@wpi.edu Sent by: histonet-boun...@lists.utsouthwestern.edu 09/03/2009 01:09 PM To histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu cc Subject [Histonet] Decal Solution Hi Histo world, I have a crazy question, I work in research facility and one of the investigators needs decal done on mice legs, they never check to see if we had decal solution before they began the procedure, of course we don't. I tried different companies to order some but they can't overnight the solution because it is a hazard. Does anyone have a protocol that they use in making a decal solution? The old histology books do but I was looking for maybe an updated solution. Please send my your suggestions they want this done ASAP. Thanks, Sharon Shaw WPI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Best IHC stainer
Agreed. Intellipath from Biocare. Load it and walk away - and it has a voice notification to tell you the run is finished. You can load it in the PM, and take slides off in the AM. Love it. Has a chilling station for AP chromogen, too. Jacke O' godsgal...@aol.com Sent by: histonet-boun...@lists.utsouthwestern.edu 08/27/2009 10:05 AM To gareth.da...@hotmail.com, histonet@lists.utsouthwestern.edu cc Subject Re: [Histonet] Best IHC stainer Ventana reagents eem to be a little pricey, but if you want a system that you can load and walk away from, it is the way to go. DAKO is an easy enough to use instrument and is an open system, but they are going to a price per slide kind of thing, if I remmeber correctly. I prefer the IP from Biocare, as is is an open system and a continuous load instrument. And it mixes the chromagen online -Original Message- From: Gareth Blaeuer Davis gareth.da...@hotmail.com To: histonet@lists.utsouthwestern.edu Sent: Wed, Aug 26, 2009 8:04 pm Subject: [Histonet] Best IHC stainer he lab I work in has been doing demos on IHC stainers Ventana Benchmark XT and ako's Autostainer Plus. We could really use feed back from current users of both. We are having a hard time deciding between the two, o any input would be great. What are the Pros and Cons with both. Thanks, Gareth Blaeuer Davis, B.S., HT _ otmail® is up to 70% faster. Now good news travels really fast. ttp://windowslive.com/online/hotmail?ocid=PID23391::T:WLMTAGL:ON:WL:en-US:WM_HYGN_faster:082009___ istonet mailing list isto...@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CD31 working protocol
Biocare has a CD31 anti mouse that does stain FFPE.I personally think nothing will ever compare with the Santa Cruz goat that no longer is available - but Biocare does work. Zerfas, Patricia (NIH/OD/ORS) [E] zerf...@ors.od.nih.gov Sent by: histonet-boun...@lists.utsouthwestern.edu 08/10/2009 01:50 PM To 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu cc Subject [Histonet] CD31 working protocol Dear Listservers, I have tried MANY protocols and I am unable to obtain a working protocol for the CD31 antibody. I have tried both the CD31 rat monoclonal and a CD31 rabbit polyclonal. Thanks in advance for your help. Patricia Zerfas National Institutes of Health Building 28A, Room 112 28 Library Drive Bethesda, MD 20892 ph: (301) 496-4464 fax: (301) 402-1068 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] reagents for IHC
Biocare. Biocare. Biocare. Margaryan, Naira nmargar...@childrensmemorial.org Sent by: histonet-boun...@lists.utsouthwestern.edu 08/03/2009 03:19 PM To histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu cc Subject [Histonet] reagents for IHC Hi everyone, How are you experiencing the economic pressures and price changes for REAGENTS? I am sorry, but I just bought reagents from DAKO and, for the price I paid for 125 ml before, I got 15-50 ml :( I am ready to switch to another company, but I need your suggestion about reagents for IHC and companies you are purchasing from: Peroxidase Block, Protein Block, Antibody diluents, DAB, AEC, different secondaries and tertiaries. Thanks in advance, Naira ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Biocare IntelliPATH Flex Immunostainer
We have had it since February-ish. Love it. No problems. Great customer service. It talks to you, too - like my GPS. Jackie O' Jessica Piche jessgro...@yahoo.com Sent by: histonet-boun...@lists.utsouthwestern.edu 07/28/2009 01:44 PM To histonet histonet@lists.utsouthwestern.edu cc Subject [Histonet] Biocare IntelliPATH Flex Immunostainer Hi All, We are just wondering if anyone out there is using the Biocare IntelliPATH Flex Immunostainer? If so how do you like it? Any problems? How long have you had it? And any other information you could provide about it would be great. Thanks, Jessica Piche-Grocki, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Modified Davidson's fixative use
To not answer your question - we fix eyes and testes in Modified Davidsons for 48 hours then transfer to formalin to await processing. Since mDF is basically formalin, alcohol, and gaa - a water rinse step is pretty moot. Johnson, Teri t...@stowers.org Sent by: histonet-boun...@lists.utsouthwestern.edu 06/08/2009 10:56 AM To histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu cc Subject [Histonet] Modified Davidson's fixative use Dear colleagues, For those who use modified Davidson's fixative for your samples, do you do a water rinse prior to starting them in alcohol dehydration series, or simply go from fixative into the alcohol? Thanks! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] nice one Kemlo
My grandfather from Slovakia was Jan. My name is Jackie, I have five sisters, two are Jeri and Stevie. My parents had a son after they had five daughters and named him Ernie.Sometimes people just run out of good names for their kids.I happen to like Rene'. It beats Apple or Moon Unit Zappa. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Leica Bond Max - any opinions?
I agree with Linda. I researched 5 different automated IHC stainers to be used for toxicology research applications, and found the intelliPATH to be the best solution for our needs. Biocare, as well, has a vast array of anti rat and mouse primary antibodies and detection systems. Additionally, the cost for the intelliPATH is quite reasonable, compared to the other platforms. Jackie O' Blazek, Linda lbla...@digestivespecialists.com Sent by: histonet-boun...@lists.utsouthwestern.edu 04/01/2009 06:22 AM To 'Michelle MacVeigh-Aloni' macve...@usc.edu, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu cc Subject RE: [Histonet] Leica Bond Max - any opinions? I would really suggest you look closely at the Intellipath from BioCare. They are a truly open system. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lbla...@digestivespecialists.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michelle MacVeigh-Aloni Sent: Tuesday, March 31, 2009 8:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Bond Max - any opinions? Hi all, I am researching the Leica Bond Max immunostainer and I like its features as advertised. The advertisement however states that the system is completely open, but after discussing it in length with the Leica rep I am finding that this is actually not so... We are a research core and work with mice and rats. Most of the pre-made antibody are designed towards human tests, so we won't use them. Then we would still be stuck with lots of money for consumables (as I understood all sold as kits with the antibodies included). We won't be using any of the antibodies, only the rest of the solutions... Is there a way out of this trap? Is there something that I didn't understand? My question is: Does anybody use Leica Bond as an open system and use other companies antibody, buffers and antigen retrieval protocols? If you do, what have you done so far? (Would you share your protocols?) We like the BioCare products and our helpful rep :-) Did anybody purchase the Research dongle? Do we really need it? How did it help you improve your applications? I was told that depending on what I want unlocked, we would have to spend $14000 to $27000 extra for it. OUCH!!! This is not a hospital where we can charge the insurance or patients for this... I need this info ASAP, because we have to make our decision in a day and there is no time to demo the unit (nor anything else). I understand that it is great for pathology, but how about animal research? It costs A LOT!!! We better know what are we getting ourselves into. Please help! Any advise and suggestions that I can get would be highly appreciated. Michelle Mac Veigh Aloni MS, HTL USC School of Medicine Department of GI and Liver Los Angeles, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Intellipath Instrument
We have also recently purchased this instrument. I highly recommend it. Sheila Haas micropathl...@yahoo.com Sent by: histonet-boun...@lists.utsouthwestern.edu 03/20/2009 09:28 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Intellipath Instrument A colleague of mine is looking into purchasing an immuno stainer. Biocare has offered a good price on the Intellipath instrument, antibodies and reagents. While we know the antibodies are reagents are excellent, we know nothing about their instrumentation. Does anyone have any first hand experience with the Intellipath instrument, service from Biocare, etc...? Any information would be helpful. Thank you in advance. Sheila Haas Laboratory Supervisor Micro Path Laboratories ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Mosquito GI Tract
More interesting - why is someone even looking at the mosquito GI tract? What happens in there that we need to know about? I guess for all the diseases those filthy beasts carry - they must harbor viruses and bacteria somewhere. Did you know that mosquitos did not exist in Hawaii until a little more than 100 years ago? Durn Westerners brought them in the barrels of drinking water they carried on their ships. Welcome to paradise. Oh - they also brought measles. Thanks a lot. Merced Leiker lei...@buffalo.edu Sent by: histonet-boun...@lists.utsouthwestern.edu 03/19/2009 02:11 PM To Breeden, Sara sbree...@nmda.nmsu.edu, histonet@lists.utsouthwestern.edu cc Subject Re: [Histonet] Mosquito GI Tract lol...I wanted to say the same thing...and how in the world can you even see it when you section it? How do you stain it without losing it from the slide? Is there really enough surface area for it to adhere and withstand washing? ...and it's ALMOST Friday...! --On Thursday, March 19, 2009 12:09 PM -0600 Breeden, Sara sbree...@nmda.nmsu.edu wrote: And here I thought I had an unusual project today, finding out as I did that my boss is thinking of examining up to 1000 cattle for TB (at 10 lymph nodes/beast) using AFB and asking me to cost it for him. I think the Mosquito GI Tract Processing and Embedding question has my project beat! How would you even KNOW you had the darned GI tract to begin with? Are these Texas Mosquitos? I'm just sayin'... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fluoro Jade Query
What's the latest and greatest on Fluoro Jade for degenerating neurons? I've found a few references, but they are old - - so am I - just celebrated my birthday - - - - anyway - -who's got the best answer? Winner gets a Hershey Bar! Jackie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Looking for Darcy Peat
I worked with Darcy in Hawaii years ago, and I think someone said she may have moved to Washington State. Darcy, are you out there? Does anyone know where she is? Jackie O' ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CD31 on FFPE mouse tissue
Biocare Medical is supposed to have a good CD31 for FFPE mouse. I don't have proof of this, but I would contact Biocare. Kim Merriam kmerriam2...@yahoo.com Sent by: histonet-boun...@lists.utsouthwestern.edu 02/13/2009 12:27 PM Please respond to kmerriam2...@yahoo.com To Histonet histonet@lists.utsouthwestern.edu cc Subject [Histonet] CD31 on FFPE mouse tissue What is everyone doing for CD31 on FFPE mouse tissue? We are looking to do IF, but a DAB protocol would be just as good. Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Off topic - professional licenses.
Wow. My last hair stylist/colorist and I obviously did not share the same vision of what my hair should look like. I wonder what governing body licenses hairdressers, and what is the criteria? How many of them have college? Soon in Illinois you will have to have a license to breed dogs, but you can still be a histotech without one.People should have licenses before they have a child - or 14. Flaming and it's only Wednesday. Jackie O' Horn, Hazel V hor...@archildrens.org Sent by: histonet-boun...@lists.utsouthwestern.edu 02/11/2009 11:13 AM To Breeden, Sara sbree...@nmda.nmsu.edu, histonet@lists.utsouthwestern.edu cc Subject RE: [Histonet] Uncertified Techs I'm fairly certain all hair dressers are licensed. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 1 Children's WaySlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, February 11, 2009 11:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Uncertified Techs Strangely enough (but not entirely unexpectedly), beauticians and barbers in this State must be licensed. Histotechs? Nope. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Legal Question
Or you can just ask the supplier to take it back. Jessica Piche jessgro...@yahoo.com Sent by: histonet-boun...@lists.utsouthwestern.edu 01/23/2009 11:38 AM To histonet histonet@lists.utsouthwestern.edu cc Subject [Histonet] Legal Question Hi, We have an antibody that we ordered in error. We would like to see if another lab would like to purchase it from us. Is that legal to ask this on the Histonet? Thanks! Jessica Piche-Grocki, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] prion contaminated tissue processing
Viable prions have been found in 20 year old paraffin blocks.Only extended exposure to bleach will kill 'em - last I heard. Not formalin, not processing - nothin. Jackie O' Rene J Buesa rjbu...@yahoo.com Sent by: histonet-boun...@lists.utsouthwestern.edu 01/15/2009 11:32 AM Please respond to rjbu...@yahoo.com To histonet@lists.utsouthwestern.edu, Joe Hardin har...@oncology.wisc.edu cc Subject Re: [Histonet] prion contaminated tissue processing NO, it will not. René J. --- On Thu, 1/15/09, Joe Hardin har...@oncology.wisc.edu wrote: From: Joe Hardin har...@oncology.wisc.edu Subject: [Histonet] prion contaminated tissue processing To: histonet@lists.utsouthwestern.edu Date: Thursday, January 15, 2009, 10:29 AM Hi All, Does anyone know if tissue processing for paraffin embedding will render prion infected tissue safe for sectioning? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] H-QIP - spleens
OK - my question is - - what in the heck do you need to examine the ENTIRE spleen for that would require breadloafing the whole thing for fixation? I was in hospital histology for a couple hundred years, and as I recall in my feeble, senility- prone years, most of the spleens that made it to pathology were from trauma. The only time I've ever seen pathology in a spleen was when my dog had a primary splenic tumor (he died). Is there some new anomaly that happens in spleens these days that require the entire spleen to be examined? Just curious. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Sakura tape problem
I would remove the tape with acetone and clear in xylene, then recoverslip with conventional coverslips. I've seen problems with refractivity with the tape, anyway. Happy Turkey Day. Jackie O' Cheryl Crowder [EMAIL PROTECTED] Sent by: [EMAIL PROTECTED] 11/21/2008 01:20 PM To Histonet [EMAIL PROTECTED] cc Subject [Histonet] Sakura tape problem Hi - I have just been given a box (100 slides) cover slipped with Sakura tape. All the tapes have been loosened from the slides with the tissue attacked to it. The tape has also curled (arched). What is the best method for reattaching the tapes to the slides so they can be viewed again. Or can it? The pathologists are really upset over these slides as they are for continuing education and cannot be replaced. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Sakura tape problem
Ah - sorry, didn't read that well. Nevermind. Weems, Joyce [EMAIL PROTECTED] 11/21/2008 01:36 PM To Jackie M O'Connor Jackie.O'[EMAIL PROTECTED], Cheryl Crowder [EMAIL PROTECTED] cc [EMAIL PROTECTED], Histonet [EMAIL PROTECTED] Subject RE: [Histonet] Sakura tape problem How do you do this with the tissue attached to the tape? Just curious... j -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Jackie M O'Connor Sent: Friday, November 21, 2008 2:32 PM To: Cheryl Crowder Cc: [EMAIL PROTECTED]; Histonet Subject: Re: [Histonet] Sakura tape problem I would remove the tape with acetone and clear in xylene, then recoverslip with conventional coverslips. I've seen problems with refractivity with the tape, anyway. Happy Turkey Day. Jackie O' Cheryl Crowder [EMAIL PROTECTED] Sent by: [EMAIL PROTECTED] 11/21/2008 01:20 PM To Histonet [EMAIL PROTECTED] cc Subject [Histonet] Sakura tape problem Hi - I have just been given a box (100 slides) cover slipped with Sakura tape. All the tapes have been loosened from the slides with the tissue attacked to it. The tape has also curled (arched). What is the best method for reattaching the tapes to the slides so they can be viewed again. Or can it? The pathologists are really upset over these slides as they are for continuing education and cannot be replaced. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: AW: [Histonet] Control slide storage
CD31 for sure. I do recall most surface markers I used tended to be unstable. Jackie O' Gudrun Lang [EMAIL PROTECTED] Sent by: [EMAIL PROTECTED] 11/20/2008 09:17 AM Please respond to [EMAIL PROTECTED] To histonet@lists.utsouthwestern.edu cc Subject AW: [Histonet] Control slide storage Hi Patsy, I am interested in the names of this certain epitopes, that loose stainability after long time storage. And I'd like to ask all the other listmembers about their experiences with this issue. Perhaps we can make a list of dangerous epitopes. Regards Gudrun Lang Akh Linz, Austria -Ursprüngliche Nachricht- Von: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] Im Auftrag von Patsy Ruegg Gesendet: Donnerstag, 20. November 2008 15:09 An: [EMAIL PROTECTED]; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] Control slide storage I store all cut paraffin sections for IHC control at 4dc and without heating to melt the paraffin, even with that I have seen some loss of antigenicity over long periods of time for certain antibodies. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 [EMAIL PROTECTED] www.ihctech.net www.ihcrg.org -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of [EMAIL PROTECTED] Sent: Wednesday, November 19, 2008 2:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Control slide storage The issue was brought up in the lab regarding the storing of IHC control slides. What experience has anyone had on the effects of long term storage and staining quality. We store the slides non preheated (with the paraffin still on them). Cindy J Chard-Bergstrom ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Leica Slide Writer
We label tens of thousands of slides a year - no paper labels required. I visited a research lab in Germany - they labele 1,000 slides a day. Never had a problem with the ink coming off or disappearing - where would it go? Patricia Karlisch [EMAIL PROTECTED] Sent by: [EMAIL PROTECTED] 11/20/2008 12:10 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] RE: Leica Slide Writer Just out of curiosity. When the slide writer is working okay, do you still have to place paper labels on the slides or does the ink disappear with time??? Thank you, Pat Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: [EMAIL PROTECTED] *E-Mail Confidentiality Notice* This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] breast bx processing
How long are they in molten paraffin Angela Bitting [EMAIL PROTECTED] Sent by: [EMAIL PROTECTED] 11/17/2008 02:27 PM To Michele Margiotta [EMAIL PROTECTED], Gary Martin [EMAIL PROTECTED], [EMAIL PROTECTED] cc Subject RE: [Histonet] breast bx processing We have staff in on Sunday afternoon, so we start them Friday night and let them sit in paraffin until the techs come in on Sunday. The extra paraffin has actually cut down the number of dry outs we were having. Martin, Gary [EMAIL PROTECTED] 11/17/2008 2:14 PM We come in and process them ! -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Margiotta, Michele Sent: Monday, November 17, 2008 9:37 AM To: [EMAIL PROTECTED] Subject: [Histonet] breast bx processing Hi All, We are trying to figure out how to adjust our processing schedule for breast core bxs on the weekends so that they are not sitting in formalin for too long. Just wondering what everyone else is doing. Your input would be very helpful. Thanks! Michele Margiotta BMHMC Histology Supervisor 631-654-7192 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] old electron microscopes
Could you imagine these things on Ebay? Joe [EMAIL PROTECTED] Sent by: [EMAIL PROTECTED] 11/14/2008 11:21 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] old electron microscopes A freind askded me to post this question to the list and see if he could get any guidance. He has two electron microscopes that appear to this untrained eye to be older than the hills, or quite possibly alien artifacts. One is a Siemens unit (power supply is bigger than a fridge). The other, I believe is also a siemens unit, but it appears newer (relatively), than the other. It's a bit smaller and has an orange body to the tube part. He wants to get rid of them and wants to know if they have any value on today's market? I tried the histoauction, but the page did not seem be working. Any help would be appreciated. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] 4 Day Weekend
If it's antigenicity you're concerned with (we had a 48 hour max fix time) - set your processor to transfer the tissues to 70% alcohol and hold them there until time to process for Monday a.m. Ta-daa! Cindy DuBois [EMAIL PROTECTED] Sent by: [EMAIL PROTECTED] 11/12/2008 01:44 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] 4 Day Weekend For the first time in my histo career, our lab will be closed the day after Thanksgiving. I am concerned about tissue sitting in the processors (in formalin) for that long. What are other labs doing? Cindy DuBois Integrated Pathology Stockton, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Is it Formalin-Without Smelling It?
Years ago, we added methyl violet to our formalin which enabled us to quickly identify the solution in or out of a container. It also serves as a pH indicator, turns yellow when acidic. That came in handy for long term fixation of large bloody specimens that might have required a formalin change to ensure good fixation. JR R [EMAIL PROTECTED] Sent by: [EMAIL PROTECTED] 11/07/2008 02:21 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] RE: Is it Formalin-Without Smelling It? My favorite method is to have the container LABELED. Jerry Ricks Research Scientist University of Washington Department of Pathology From: [EMAIL PROTECTED] To: [EMAIL PROTECTED]; histonet@lists.utsouthwestern.edu Date: Fri, 7 Nov 2008 13:34:43 -0600 CC: Subject: [Histonet] RE: Is it Formalin-Without Smelling It? I knew there was a trick, I just couldn't remember what it was. Thanks everyone! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Charles, Roger Sent: Friday, November 07, 2008 1:43 PM To: Smith Wanda; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: Is it Formalin-Without Smelling It? We are using Shiffs reagent. When you put a drop of formalin in Shiffs reagent it turns purple. Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Smith Wanda Sent: Friday, November 07, 2008 1:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Is it Formalin-Without Smelling It? Happy Friday Everyone! Silly question: Is there an easy way to tell if a solution in a container is/is not formalin without smelling it? Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ Windows Live Hotmail now works up to 70% faster. http://windowslive.com/Explore/Hotmail?ocid=TXT_TAGLM_WL_hotmail_acq_faster_112008___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] embedding
2-3 hours. KELLY BOYD [EMAIL PROTECTED] Sent by: [EMAIL PROTECTED] 11/06/2008 12:25 PM Please respond to [EMAIL PROTECTED] To histonet Histonet@lists.utsouthwestern.edu cc Subject [Histonet] embedding Just trying to get some feedback on how much time (estimated) it should take an average histotech with experience to embed 150 shave biopsies. Let us just say the majority have multiple pieces to be embeded on edge. Any clue? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com Tele (252)-830-6866 Cell (252)-943-9527 Fax (252)-830-0032 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] progressive HE staining using Gills
Richard Allen 7211 Hematoxylin doesn't stain mucin blue, and is a beautiful all around nuclear stain.I'm sure they'll send you a sample. Mickie Johnson [EMAIL PROTECTED] Sent by: [EMAIL PROTECTED] 10/30/2008 02:16 PM Please respond to [EMAIL PROTECTED] To 'Angela Bitting' [EMAIL PROTECTED], histonet@lists.utsouthwestern.edu cc Subject RE: [Histonet] progressive HE staining using Gills Angela, Were you using Harris before? If so, the difference is that Gill formulations stain the mucin of goblet cells quite a dark blue and many GI docs don't like that look. They can get used to it though as ours did at Sacred Heart Medical Center. Hope this helps. Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com www.mohslabstaffing.com Email: [EMAIL PROTECTED] DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Angela Bitting Sent: Thursday, October 30, 2008 11:30 AM To: histonet Subject: [Histonet] progressive HE staining using Gills We just switched our HE stain to a progressive stain using Gill II hematoxylin. Our derm guys love it, but now our GI docs are screaming bloody murder. Does anyone have suggestions for a protocol that will make both 'relatively happy'?? Thanks for letting me pick your brains again!!! Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.175 / Virus Database: 270.8.5/1756 - Release Date: 10/30/2008 2:35 PM ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Protect fingers during Microtomy
My Dad was a workman's comp expert for a large insurance company. In the early 90's, I was managing a histo lab where the head pathologist (funny, a neuropath) thought it would be cheaper to resharpen stainless steel microtome knives instead of using disposable blades.(I had a previous nasty injury from a steel knife). Dad put together an estimate on each individual finger of how much $$ the hospital would have to pay out in the event of major lacerations or amputation. We also included feet - since those heavy knives would often hit the floor when you had to jump out of the way if someone mishandled one. Turned out, we never used stainless steel knives again.It was cheaper to use disposables than for one person to lose the use of a thumb. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Toluene vs. Xylene
Yeah - but since Toluene is the major component of airplane glue- at least you'll be happier using toluene over xylene. Jackie Rene J Buesa [EMAIL PROTECTED] Sent by: [EMAIL PROTECTED] 10/09/2008 09:38 AM Please respond to [EMAIL PROTECTED] To histonet@lists.utsouthwestern.edu, James Dooley [EMAIL PROTECTED] cc Subject Re: [Histonet] Toluene vs. Xylene Not really and both are as dangerous and both should be avoided. As a matter of fact xylene began to be used in the mid 1950s to substitute chemicals like toluene, until it was found that both were equally dangerous. Toluene is present in many mounting media. René J. --- On Thu, 10/9/08, James Dooley [EMAIL PROTECTED] wrote: From: James Dooley [EMAIL PROTECTED] Subject: [Histonet] Toluene vs. Xylene To: histonet@lists.utsouthwestern.edu Date: Thursday, October 9, 2008, 10:16 AM Is there a significant difference between using xylene and toluene when deparaffinizing embedding tissue? Thank you, James ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
