RE: [Histonet] Peloris Users
We have helped that problem by being sure there is adequate graded alcohols on the Peloris. There must be between 60-70%, 80-90%, and 95% before you have the 100% alcohols. If the concentration's are too high there is inadequate infiltration of the tissue's. We usually only use the 2 hour and the 8 hour processing factory default. Unless, we have a stat. We have also noticed if you wait until Prompted to change the lowest alcohol at 51% we see degradation in our staining as well. We are not letting it get below 58-60% before we change an alcohol out. I hope this helps, Josie Britton HT Cheshire Medical Center 580 Court Street Keene, NH 03431 603-354-5454 ext.2454 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Thursday, July 08, 2010 9:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Peloris Users To all you Peloris users, here is my delimma. When we follow the machines prompt to change the solutions because of purity and we then process small biopsies on the machine, it overprocesses the specimens. So the staff have been overrriding the prompt to change the solutions causing specimens to be processed in impure solutions causing a host of other problems. Have any of you out there with Peloris experience had this problem? Is it a machine problem that should be fixed or human error due to overriding the prompts? I am new to this position and have very little experience with this issue so any help or suggestions will be greatly appreiciated. Thank you. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-dev...@carle.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (no subject)
We retain our containers and specimens for 3 weeks. Josie -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Feher, Stephen Sent: Wednesday, June 23, 2010 5:51 PM To: Hartz, Rhonda SktnHR; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (no subject) We retain them until the specimen is signed out, usually no more than 3 days. This has been helpful if the specimen container labeling is called into question either by the pathologist (because the cellular profile does not match what the specimen source indicates) or the clinician. Steve -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hartz, Rhonda SktnHR Sent: Tuesday, June 22, 2010 5:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi. This is my first time, so I apologize if I am not clear enough. We have had a request from one of our pathologists to retain empty specimen containers after grossing is complete. Is anyone aware of any recommendations, or does anyone out there retain their empty specimen containers? Rhonda Hartz Technologist Supervisor Anatomic Pathology Division Saskatoon Health Region (306) 655-8197 rhonda.ha...@saskatoonhealthregion.ca ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] slim jims
We put them in the formalin first just like a specimen. Josie -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret Sent: Tuesday, June 22, 2010 2:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slim jims Do you put the slim jims in formalin and then process them or just put them in the processor? Margaret ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Help! In need of positive Gram Control
It's true! Who knew that Gram + and- riddles meat sticks could be so yummy and versatile too! Josie -Original Message- From: Gill, Caula A. [mailto:cg...@marylandgeneral.org] Sent: Tuesday, June 22, 2010 10:16 AM To: Josie Britton; dianar...@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Help! In need of positive Gram Control You have got to be kidding!! That's hysterical. So process a slim jim and you have Gram - and + controls. If you're serious I'm trying it. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Josie Britton Sent: Tuesday, June 22, 2010 6:10 AM To: dianar...@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Help! In need of positive Gram Control Have you tried a Slim Jim? They have gram positive and negative rods in them. Regardless, I still enjoy eating them once and a while! Josie Britton Ht Cheshire Medical Center Keene, NH 03431 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of dianar...@aol.com Sent: Monday, June 21, 2010 7:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help! In need of positive Gram Control Help! We are in need of positive Gram Control Blocks if anyone has any extra they are willing to part with. I have lots of Fungus, Pneumocystis and HPV tissue blocks to trade. Diana Ripley John Muir Histology Concord Campus 2540 East Street Concord, CA 94520 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Breezy lab/flyaway ribbons!
We have been having trouble with big breeze blowing our precious ribbons out of our hands while cutting. We would like a door on the Histology lab to cut down on the breeze of people walking by through the hall. Our facilities want to find out what other people are doing to stop this problem. We also have air ducts blowing down from above, which is not helping the problem. We would like as many labs solutions as possible. Our facilities have come up with all these crazy barriers that we would have to move to walk around when we need to put our racks on the stainer, answer timers, print more slides, use the oven, etc... Any input would be appreciated! Breezy girls, Josie Britton and Chris Braaten Cheshire Medical Center Keene, NH 03431 CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: AMT Cover
I second that! Josie Britton HT Keene, NH -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Friday, May 14, 2010 11:54 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: AMT Cover All, I just looked at the notorious AMT cover that people are up in arms about and I must disagree with the comments that were posted thus far. The techs here and myself are quite surprised by the reactions because it is on a medical catalogue and is far from being lewd or obscene. You could probably find worse by turning on the TV for 15 minutes or looking at a billboard or two. Just Some Wisconsin Input, Glen Dawson BS, HT QIHC (ASCP) IHC Manager Milwaukee, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Xylene Recycler
We resolved this issue by placing a filter/screen in our waste to be recycled carboy. After you clean out the excess lint and Schmutz from the line you should be all set! Welcome! Josie Britton HT Cheshire Medical Center 580 Court Street Keene, NH 03431 603-354-5454 ext.2454 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Wednesday, February 10, 2010 12:58 PM To: histonet@lists.utsouthwestern.edu Cc: Patricia Webster Subject: [Histonet] Xylene Recycler Anyone using the new CBG instrument? Am having some issues with clogged lines - which we never had with the older model. Really don't want to change processes - which is what they are asking us to do. Thanks Nita Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsea...@swmail.sw.org 254-724-2438 CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] EBER ISH problems
Try switching your DAB kit to Refined Red, this helped us. We also run a negative RNA and positive RNA on the patient also. Josie Britton HT Cheshire Medical Center Keene, NH -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Goodacre, Suzanne Sent: Wednesday, January 20, 2010 11:20 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] EBER ISH problems Hello, Our Histo lab currently uses the Leica Microsystems Bond instrument for EBER ISH. We noticed that eosinophils readily pick up the DAB detection reagents that we use. I've attempted to alter the EBER ISH protocol on the BOND so that the eosinophils don't take on the detection reagents but I'm not having much luck. Does anyone have any tips on how to prevent or avoid the problem? Suzanne Goodacre Research Technologist Micro Molecular Lab Children's Hospital of Wisconsin 414-266-2709 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] fish scales
Nair hair remover works great on toenails! Josie Britton HT -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret Sent: Thursday, December 10, 2009 3:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fish scales We are trying to cut fish scales that have been decalcified. They are chunking out when we try to cut them. I think we need to soften the keratin and I looked in the archives for the right dilution of ammonium hydroxide to use. One post said 5% the other said straight. What dilution do you recommend? I'm think it would probably be the same as what you use on toenails. Margaret Perry HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC Patient Negatives
Hi All, I have an issue with my computer, so I cannot see past the little paragraphs on the Histonet archives. Sorry! This question is probably asked many, many times. When we run our IHC's we always have a patient negative slide to go with our case. We run everything accept Herceptin on the Bond. When running a patient's IHC on the bench and on the Bond we use a negative patient control for both. (We run a test control on all antibodies also) If we are running a double stain IHC also we run another negative patient control for that stain also. The debate we are having is, if the next day you run another IHC on the same patient using the same DAB define kit, should we be running another negative patient control? Thanks for your help! Josie Britton HT Cheshire Medical Center CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC Zinc Fixative supplier/source
We use Z-Fix from Anatech LTD, 269-964-6450. Josie Britton HT -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cormier, Kathleen Sent: Thursday, October 15, 2009 8:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Zinc Fixative supplier/source Hello Netters, I am searching for a vendor to supply IHC Zinc fixative. Not zinc formalin. It's ALMOST like the BD Pharmingen IHC zinc fix, but contains Zinc acetate too. We do not want to make it up in house thoughit contains calcium acetate, zinc acetate, zinc chloride and tris buffer. I have searched the 'net and all I garnered was a head ache, there are so many variations of zinc fixatives, and none are what we need. Any help would be appreciated. Thank you! Kathy Cormier Histology Manager Charles River Laboratories 251 Ballardvale Street Wilmington, MA 01887 Ph: 781-222-6803 Fax: 978-988-8793 kathleen.corm...@crl.com BLOCKED::mailto:kathleen.corm...@crl.com Experience the new www.criver.com http://www.criver.com/ Accelerating Drug Development. Exactly. Notice - This email and any files transmitted with it are confidential and may contain privileged and/or proprietary information. You must not disclose this message to another party without Charles River's express written consent. If you are not the intended recipient you must not copy, distribute or use this email or the information contained in it for any purpose other than to notify us. If you have received this message in error, please notify Charles River immediately, and delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Leica Bond/Novocastra Reagents
We get almost all their antibodies and ancillaries and have never had a problem! They are a great company and we love the Bond Max. Josie Britton HT Cheshire Medical Center Keene, NH 03431 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Monday, October 05, 2009 11:20 AM To: histo...@pathology.swmed.edu Subject: [Histonet] Leica Bond/Novocastra Reagents Hello, We are considering the Leica Bond and their reagent rental or acquisition option, and I have a question regarding back orders. If anyone places an order through Leica for their Bond reagents (novocastra), are there any problems with back orders on antibodies, detection system and other reagents needed to run the Bond? Or, do you usually get the items right away? Thank you, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] water on slides?
When this happens in our lab, we put Drierite (anhydrous calcium sulfate) into the clearing agents. Just put enough to have not quite a solid layer on the bottom of the staining well, so it will not block the staining rack from going all the way into the clearing agent. We get ours from W.A. Hammond Drierite Company LTD, Xenia, Ohio 45385. Telephone number 937-376-2927. I hope this helps! Josie Britton HT Cheshire Medical Center Keene, NH 03431 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hayes, Tina J. Sent: Monday, August 24, 2009 12:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] water on slides? We are having a problem with what seems to be water on our slides. We have had very high humidity levels in the air. It seems that we look at the slides before taking them to the pathology office, one pathologist reviews them and sees little or no water droplets, but as they sit and another pathologist reads them later, like the following day, the droplets are very apparent. We use Clearite-3. We have no xylene in our lab. We also coverslip with Permount. Is it possible that the clearite-3 and/or Permount is absorbing moisture from the atmosphere to the slides? And if so, do you have any suggestions for combating this issue? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] LYVE-1 antibody
We have one and we love it! Our Pathologists keep adding more and more antibodies to our list. It is so easy to program. Leica's support staff are great too! Josie Britton HT Cheshire Medical Center Keene, NH 03431 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha Ward Sent: Tuesday, August 11, 2009 2:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] LYVE-1 antibody Hello, I wanted to know if anyone has tried this antibody on the Bond stainer, and if so, how did it work for you? I have purchased one from Santa Cruz Biotechnology (cat#sc-80170) at the request of one of our Pathologists. Any feedback is appreciated. Thanks! Martha Ward Molecular Diagnostics Lab Wake Forest University Baptist Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ISH
Try Novocastra and Bond Reagent products 2009. 800-248-0123 ext. 7880. I hope this helps! Josie Britton HT Cheshire Medical Center Keene, NH 03431 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Tuesday, August 11, 2009 2:36 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] ISH Can anyone supply me with a vendor for Kappa/Lambda ISH? I heard that Dako and Ventana have had to take their ASR Kappa.Lamda product off of the market. I am in need of where to go for ASR or IVD in this area!! Thanks, Dorothy Webb, HT Regions Histology Technical Supervisor 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Blue haze
I know this! We have had this problem before. We have now added and extra cap full ( or ½ cap full per SurgiPath) to our Define. So instead of 2 we put 3 in. We also have a problem with our water filters clogging. Make sure you have plenty of water pressure also, but this pretty much solved our problem. Josie Britton HT Cheshire Medical Center 580 Court Street Keene, NH 03431 603-354-5454 ext.2454 -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Bernice Frederick Sent: Thursday, December 04, 2008 9:06 AM To: 'Joe Nocito'; 'Histonet Alias'; 'Marshall, Kimberly' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blue haze I'm sure you'll just charm them and they'll never catch on Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Joe Nocito Sent: Wednesday, December 03, 2008 8:31 PM To: Histonet Alias; Marshall, Kimberly Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Blue haze I thought that was Purple Haze, by Jimi Hendrix. Oh dear me, is it Friday yet? Forgive me, we are in the window for CAP and I'm just all in a haze. JTT - Original Message - From: Histonet Alias [EMAIL PROTECTED] To: Marshall, Kimberly [EMAIL PROTECTED] Cc: histonet@lists.utsouthwestern.edu Sent: Wednesday, December 03, 2008 12:08 PM Subject: Re: [Histonet] Blue haze Hi Kimberly, Are you using a clarifier step? On Wed, Dec 3, 2008 at 12:47 PM, Marshall, Kimberly [EMAIL PROTECTED] wrote: Howdy all, I have just changed over to Surgi Path's H E system. With the latest fear of running out of hematoxylin and all my Pathologist wanted us to change over. Its a great stain but we have a bad haze in the background. It is not hurting the tissue or DX at all, but to ME it looks awful. Is there anyone else out there using this? We are not using positive charged slides or anything added to my water bath. Any suggestions Thanks in advance Kimberly Marshall HT (ASCP) == The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. == ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Al Ias HT(ASCP) Histology Manager Pathology Laboratory United States ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Uneven HE Staining
Sounds to me that your having a de-paraffining problem. Check your xylene or clearing agent, maybe there is water in them or they are not as clean as they should be. Are you on a daily rotation/change of staining/de-paraffin reagents? Maybe, your oven isn't hot enough to melt some paraffin off the slides before de-par! Josie Britton HT -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Gudrun Lang Sent: Wednesday, November 19, 2008 9:48 AM To: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] RE: Uneven HE Staining Are the coplin jars all equally filled? If the wash-jars are partly filled, the lower slides would be more differentiated than the upper. Gudrun -Ursprüngliche Nachricht- Von: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] Im Auftrag von Karla Arrington Gesendet: Mittwoch, 19. November 2008 02:34 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] RE: Uneven HE Staining Recently we have had an issue with uneven HE staining. Lately slides that have three sections on one slide, mostly GI biopsies, and the last section (bottom of slide) are paler than the upper two section. All cut by the same person and the stains were great. What could be the cause of this? Also with the quality of formalin. Can formalin be purchased as bad when it takes 6 days to fix placenta or breasts? What is the cause? Karla Arrington, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Dako
Hi, We just got the new Bond Max IHC stainer and we love it. You just cut the slides dry them and place them on the Bond. It does retrieval, antibody staining, and counter stain. You just dehydrate , clear, and mount your coverslip. It is easy to use. It has 3 individual slide tray's of 10. You can load more slides on the empty tray's and start a new batch while the others are running. We run into the pathologist's adding more antibodies to the list an hour after we have run the first batch frequently, so this feature is great. When you add more IHC's the run time on all the slide tray's run times do increase, but it's better than having to wait another 2-3 hours to put your next set of immuno's on. Hope this helps! Josie Britton HT Cheshire Medical Center 580 Court Street Keene, NH 03431 CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
