[Histonet] Blades
I generally use CL Sturkey Extremus Low Profile Blades for pretty much everything...when I am having trouble with intestine sections, I use the DuraEdge Encore blade and it usually works very well. Lyn M. Stadler, BS, HTL(ASCP)CM Research Histotechnologist Department of Histopathology Cleveland Biolabs, Inc. 73 High Street Buffalo, NY 14203 716-849-6817, ext 417 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Tuesday, April 23, 2013 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 113, Issue 24 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: Happy Lab Week from Pam Barker and RELIA Solutions!! (Ian R Bernard) 2. staining near pap pen only (Emily Sours) 3. Re: staining near pap pen only (Emily Sours) 4. RE: Direct Immunofluorescence on Benchmark XT/Ultra (Sue Hunter) 5. RE: Direct Immunofluorescence on Benchmark XT/Ultra (Vanessa Perez) 6. RE: Direct Immunofluorescence on Benchmark XT/Ultra (Vanessa Perez) 7. RE: Direct Immunofluorescence on Benchmark XT/Ultra (Vanessa Perez) 8. Forwarded post for a histonet member: Subject: CLIA 24-hour gross review. (Sandy Cope-yokoyama) 9. RE: Blades (McAnn, Sherrian) 10. New Lab (Hale, Meredith) -- Message: 1 Date: Mon, 22 Apr 2013 17:22:13 + From: Ian R Bernard ibern...@uab.edu Subject: RE: [Histonet] Happy Lab Week from Pam Barker and RELIA Solutions!! To: Pam Barker rel...@earthlink.net, Histonet histonet@lists.utsouthwestern.edu Message-ID: d4f4c602b10b9f45b4e9271af6380e16181b7...@uabexmb1.ad.uab.edu Content-Type: text/plain; charset=iso-8859-1 - We are celebrating with sponsor breakfasts and lunches for lab personnel from local restaurants each day. - Different internal games. - Educational Lab Week information and mementos for patients and staff alike - And finally, two half days this week, some personnel will go hiking and others on another day horseback riding. That way our mission is staffed without negative impact to patient care V/r Ian R. Bernard, MSHA, HT (ASCP) NCOIC-Manager, Anatomic Pathology Lab 10th Medical Group USAF Academy, CO 80840 Graduate Certificate In Gerontology Student-UAB 210-687-7540 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pam Barker Sent: Monday, April 22, 2013 11:49 AM To: Histonet Subject: [Histonet] Happy Lab Week from Pam Barker and RELIA Solutions!! Hi Histonetters. Happy Lab Week!! Wow this year is just flying by!! It was just Histotechnology Professionals Day a short time ago and here it is lab week already! So here is my question. How is your facility celebrating Lab Week? I hope you are gearing up for a fun week and if you need some ideas for lab week check out Advance Magazine, they have some fun ideas. I think we both know it wouldn't be an email from me if I didn't tell you about my current openings. Please take a second and check them out. Here Are My Spotlight Opportunities: Night Shift Histotech - Patterson, NJ Brand New Lab!! Day Shift Histotech - Salem, VA beautiful area and great Team HT/HTL Mohs Histotech - Long Beach, CA - Make it your own brand new in office lab! The rest of the histology positions that I am most excited about are located in these areas: Nashville, TN Waco, TX Tyler, TX -2 sites one at a hospital and one at a private dermpath lab Atlanta, GA Charlotte, NC Staunton, VA Louisville, KY Staunton, VA Remember if you refer someone to me and I place them now or in the future You will earn a 500 dollar referral fee!! If you think you or someone you know might be interested in any of these opportunities or would like to talk about a job search in another area, please contact me. I can be reached at 866-607-3542 or rel...@earthlink.net?Thanks-Pam Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: rel...@earthlink.net www.facebook.com/PamBarkerRELIA
[Histonet] Processing Protocol Using Mineral Oil
We have been using this protocol on mostly mouse tissues with great results! We run this on a VIP 3000. 70% Ethanol, room temp, 1 hour 100% Isopropyl Alcohol, room temp, 1 hour 100% Isopropyl Alcohol, room temp, 1 hour 100% Isopropyl Alcohol, room temp, 1 hour 5:1 Iso:Mineral Oil, 50 degrees, 1 hour 2:1 Iso:Mineral Oil, 50 degrees, 1 hour 100% Mineral Oil, 50 degrees, 1 hour 100% Mineral Oil, 50 degrees, 1 hour 100% Mineral Oil, 50 degrees, 6 hours Paraffin, 60 degrees, 1 hour Paraffin, 60 degrees, 1 hour Paraffin, 60 degrees, 1 hour Followed by a cleaning cycle after cassettes are removed and put into molten paraffin at the embedding center Lyn M. Stadler, BS, HTL(ASCP)CM Research Histotechnologist Department of Histopathology Cleveland Biolabs, Inc. 73 High Street Buffalo, NY 14203 716-849-6817, ext 417 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, December 05, 2012 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 109, Issue 6 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: automated microtomes (Lynette Pavelich) 2. HistoTALK (David Kemler) 3. RE: Tissue Processors (Marcum, Pamela A) 4. RE: Tissue Processors (Boyd, Debbie M) 5. Re: Immunohistochemical detection of cytokines in older frozen sections (Rob Day) 6. RE: Histonet Digest, Vol 109, Issue 5 (Riesen, Rebecca) 7. Re: Fixation time (Geoff) 8. Histology Positions in Little Rock AR (Marcum, Pamela A) 9. Re: Fixation time (Teri Johnson) 10. Re: Tissue Processors (Teri Johnson) 11. job opening in PA (Hutton, Allison) 12. Re: Re: Tissue Processors (Rene J Buesa) 13. RE: Re: Tissue Processors (Ellenburg, Deborah) -- Message: 1 Date: Wed, 5 Dec 2012 12:10:06 + From: Lynette Pavelich lpave...@hurleymc.com Subject: RE: [Histonet] automated microtomes To: Rene J Buesa rjbu...@yahoo.com, Rathborne, Toni trathbo...@somerset-healthcare.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 89f4666a496dc949a819ecc40e11c867bf56c...@exchangemb1.hmc.hurleymc.com Content-Type: text/plain; charset=iso-8859-1 I am purchasing my second Leica RM2255 automated microtome. Rene' is correct in saying that the carpal tunnel syndrome will eventually affect every advanced tech by using any microtome manually. On the RM2255, the flywheel additionally is automated. You can choose to use the flywheel manually or automated by the simultaneous pushing of two buttons. Nice feature for us oldies to ease us into automation or if you have a tiny specimen that you need to take extra care with and want to use manually. Companies are very happy to send in a demo to try for a couple weeks. Happy shopping!! ;) Lynette From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Rene J Buesa [rjbu...@yahoo.com] Sent: Tuesday, December 04, 2012 1:19 PM To: Rathborne, Toni; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] automated microtomes The advantage of the so called automated microtomes (the only thing automated about them is the block advance) is that they alleviate wrist effort and in some ways prevent carpal tunnel syndrome that affects some histotechs (mostly of the senior persuasion). I would go with the Leica. Ren? J. From: Rathborne, Toni trathbo...@somerset-healthcare.com To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Tuesday, December 4, 2012 11:48 AM Subject: [Histonet] automated microtomes I'm looking for some opinions about the automated microtomes currently available. Which ones do most techs prefer? Which are more reliable? Is there an advantage to having a semi-automated microtome? Thanks in advance for your replies. Toni ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 2 Date: Wed, 5 Dec 2012 04:28:54 -0800 (PST) From: David Kemler histot...@yahoo.com
[Histonet] Portable Clean Air System
All ~ Just curious if anyone has any experience with the Airfiltronix portable fume extractor filter? I saw it in the Ted Pella catalog, item # 3120, http://www.tedpella.com/safety_html/3120.htm It is designed to be used with a microwave, but can be used anywhere, according to the catalog. We are thinking about using something like this in our processing lab due to space constraints. Any input is greatly appreciated. Lyn M. Stadler, BS, HTL(ASCP)CM Research Histotechnologist Department of Histopathology Cleveland Biolabs, Inc. 73 High Street Buffalo, NY 14203 716-849-6817, ext 417 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sakura VIP 3000 Tissue Processor
Anyone out there using a VIP 3000? I need some replacement gaskets that Sakuara no longer manufactures. Anyone know of an alternate vendor or supplier that carries them? Or, better yet, anyone have some that they are interested in giving away ;). Thanks in advance! This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Murine Intestine Protocols
Hello all ~ If anyone could share their protocols for intestine processing, embedding, cutting, etc I would greatly appreciate any input. I am having trouble getting consistently good quality intestine sections and am always looking to improve. Also, does anyone have an experience with a product called GI FIX from BBC/Histoperfect (Item # )? Lyn Stadler Histology Technician Department of Histopathology Cleveland Biolabs, Inc. 73 High Street Buffalo, NY 14203 This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Slide and Cassette Labeling
Considering Purchasing the Brady Slide and Cassette Labeling System with BSP3 Attachment Unit, the BBP11 Labler, codesoft software and bar code scanner. If anyone out there is using it, I'd love to hear some opinions! Thanks in advance, Lyn Stadler Histology Technician Department of Histopathology Cleveland Biolabs, Inc. 73 High Street Buffalo, NY 14203 This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Exam Prep Materials
Has anyone ever heard of or used the Histotechnology Exam Secrets Study Guidehttp://www.mo-media.com/histotech/? If so, I would appreciate opinions on it. Thanks! Lyn Stadler Histology Technician Department of Histopathology Cleveland Biolabs, Inc. 73 High Street Buffalo, NY 14203 This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Exam Prep Info/Guidance/Suggestions
All ~ I am about to begin preparing for the HTL certification exam. (I supposse it is only fair to mention that this is will be my second attempt. My first exam taken last month with a score of 382 out of a minimum passing score of 400). As a research Histology Technician, a good majority of the material covered on the exam was new to me. My prep for the first exam was to read Histotechnology, A Self-Instructional Text, 3rd Edtion by Carson and Hladik and then answer a lot of practice questions from the Board of Registry Second Edition Practice Questions book. I focused mostly on the special staining info and images because of the high concentration of questions on the exam as well as my limited professionnal exposure to these...unfortunately, it was still the area i did most poorly in on the exam. In order to go a little more in depth the second time around, my plan is to is to re-read each chapter ( of Carson), then assess my knowledge by being sure I can do each of the Chapter Objectives and then answer the questions in the self-assessement workbook, then answer the questions on that particular chapter topic from the Seond Edition of the Board of Registry Study Guide, Practice Questions for the Histotechnology Exams (The Purple Book). I have other textbooks at my disposal, but as a mostly visual learner, I find Carson's approach to best get the information into my brain! Again, once I feel comfortable with all the basic knowledge, I would focus mostly on staining. Specifically, I would like feedback from anyone who has recently passed the exam about my approach, and suggestions for other ideas. Also, I have very limited CAP/Joint Comission type knowlege and wonder if anyone can offer a resource for basics for someone like me in research who is not presented with the info and regulations on a daily basis! Also, any suggestions for images online of special stains would be a great resource for me. Thanks in advance for any and all info! Lyn This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Zeiss Microm Rotary Microtome HM 325
Curious if anyone out there has one of these microtomes? We have one that needs repair of the Trimming Lever. The warranty has expired and I am trying to determine the most cost effective way to deal with it? Pay for the service call or purchase a service contract. Any information/suggestions/comments would be GREATLY appreciated! Lyn Stadler Histology Technician Department of Histopathology Cleveland Biolabs, Inc. 73 High Street Buffalo, NY 14203 This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Bar Coding and Sample Tracking
Looking for advice/comments/suggestions regarding sample tracking and data management. I work for a small research institution doing mostly mouse/monkey tissues. Up until now, we have been tracking samples with an excel spreadsheet, but increasing workload is making this more and more difficult. We average probably less than 500 blocks per month (very rough estimate). Basically what I think we need is bar coding for samples and a centralized data storage place so that all the information available for a single sample is in the same place, and can be accessed by anyone anywhere, but not altered (for GLP compliance) as well as capability to expand as our workload varies. This is my first step in looking into this so any input at all is greatly appreciated! Lyn Stadler Histology Technician Department of Histopathology Cleveland Biolabs, Inc. 73 High Street Buffalo, NY 14203 This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] VIP 1000
Are you looking for the VIP 1000 User Manual? If so, I can send a copy Lyn Stadler Senior Research Associate - Histopathology Cleveland Biolabs, Inc. 73 High Street Buffalo, NY 14203 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Thursday, December 23, 2010 8:01 AM To: histonet@lists.utsouthwestern.edu Subject: [BULK] Histonet Digest, Vol 85, Issue 21 Importance: Low Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Kathleen Dittrich, where are you? (Settembre, Dana) 2. Physician signature (Webb, Dorothy L) 3. RE: Physician signature (Weems, Joyce) 4. Thomas Crowell is out of the office. (thomas.crow...@novartis.com) 5. RE: RE: Physician signature (Mike Pence) 6. RE: RE: Physician signature (Weems, Joyce) 7. High Paying Job Opportunity - Live anywhere (Alisha Dynan) 8. Fisher MicroProbe manual staining system (Martha Ward) 9. RE: RE: Physician signature (Morken, Tim) 10. Frozen Specimen Handling (John Shelley) 11. RE: RE: Physician signature (Weems, Joyce) 12. Symphony cover slipper (Clare Thornton) 13. VIP 1000 Help (Drew Meyer) 14. FW: ASCP Action Alert - Physician Signature Requirement _FYI (Liz Chlipala) 15. RE: Frozen Specimen Handling (sgoe...@mirnarx.com) 16. Used embedding center and microtome (alineum...@aol.com) 17. CAP Question regarding procedure manual (Victor Tobias) 18. RE: CAP Question regarding procedure manual (Weems, Joyce) 19. RE: CAP Question regarding procedure manual (WILLIAM DESALVO) 20. RE: CAP Question regarding procedure manual (Jesus Ellin) 21. Re: CAP Question regarding procedure manual (bsulli...@shorememorial.org) -- Message: 1 Date: Wed, 22 Dec 2010 13:18:22 -0500 From: Settembre, Dana sette...@umdnj.edu Subject: [Histonet] Kathleen Dittrich, where are you? To: Settembre, Dana sette...@umdnj.edu, 'IRENA SREBOTNIK KIRBIS' irena.kir...@hotmail.com, hfe...@jhmi.edu hfe...@jhmi.edu, ahut...@dh.org ahut...@dh.org, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: b64b947688fb794a8c191d19f22927c875be7...@umdexmbx02.core.umdnj.edu Content-Type: text/plain; charset=us-ascii Kathleen Dittrich, formerly from Dako. Where are you? Dana Settembre -- Message: 2 Date: Wed, 22 Dec 2010 12:36:43 -0600 From: Webb, Dorothy L dorothy.l.w...@healthpartners.com Subject: [Histonet] Physician signature To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Message-ID: 65365f35c0f2ef4d846ec3ca73e49c43ea95058...@hpemx3.healthpartners.int Content-Type: text/plain; charset=us-ascii According to our manager, the physician signature is for clinical testing only, not anatomic pathology. If anyone knows that this is inaccurate, would love to see the written rules!! Thanks and everyone have the most blessed Holiday!! This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 -- Message: 3 Date: Wed, 22 Dec 2010 13:38:30 -0500 From: Weems, Joyce jwe...@sjha.org Subject: [Histonet] RE: Physician signature To: Webb, Dorothy L dorothy.l.w...@healthpartners.com, 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Message-ID: 92ad9b20a6c38c4587a9febe3a30e164044b332...@chexcms10.one.ads.che.org Content-Type: text/plain; charset=us-ascii It specifically mentions AP technical charges in the proposal. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, December 22, 2010 13:37
