[Histonet] Colorado State Histo Meeting April 19-20
Hey Netters, It is not to late to join us in Fort Collins. Go to http://www.coloradohisto.org/ to register and check out the program. Respectfully, Matt Lunetta BS HT(ASCP) Lead Histology Longmont United Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] 2013 Colorado Society of Histotechnology meeting will be held April 19th 20th
The 2013 Colorado Society of Histotechnology meeting will be held April 19th 20th at the La Quinta Inn, Fort Collins, CO. The program is now posted on the CSH website. For more information regarding the meeting, accommodations, online registration and payment, please visit http://www.coloradohisto.org/2013/meeting.htm. 2013 CSH Registration Packet: http://www.coloradohisto.org/2013/2013_CSH_Program.pdf Online registration is strongly encouraged. If you have to mail or fax your registration please print legibly and provide a valid email address as receipts are no longer being mailed. If you are interested in attending the meeting please register sooner rather than later. As always, you have up until the day of the meeting to pay. Looking forward to seeing all of you at the meeting in April. Respectfully, Matt Lunetta BS HT(ASCP) Lead Histology Longmont United Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] 2013 Colorado Society of Histotechnology Meeting April 19th 20th
The 2013 Colorado Society of Histotechnology meeting will be held April 19th 20th at the La Quinta Inn, Fort Collins, CO. The program is now posted on the CSH website. For more information regarding the meeting, accommodations, online registration and payment, please visit http://www.coloradohisto.org/2013/meeting.htm. 2013 CSH Registration Packet: http://www.coloradohisto.org/2013/2013_CSH_Program.pdf Online registration is strongly encouraged. If you have to mail or fax your registration please print legibly (my eyes aren’t getting any younger) and provide a valid email address as receipts are no longer being mailed. If you are interested in attending the meeting please register sooner rather than later. As always, you have up until the day of the meeting to pay. Looking forward to seeing all of you at the meeting in April. Respectfully, Matt Lunetta BS HT(ASCP) Lead Histology Longmont United Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: Subject: [Histonet] PAS Diastase
Jennifer, No you are not. Matt Lunetta BS HT(ASCP) Longmont United Hospital Message: 9 Date: Wed, 14 Mar 2012 22:47:17 -0700 From: Jennifer MacDonald Subject: [Histonet] PAS Diastase To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Hi All, At a local lab when a pathologist orders a PAS diastase the histotechnicians do just one slide with diastase. They do not do an undigested slide. How would the pathologist know if the digested slide had a glycogen to begin with? Am I over thinking this? Thanks, Jennifer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histo Aide Duties
Hey Histo Netters, For the CAP pro's; I have been wondering what duties can a non-certified histo-aide preform in a CAP facility? Other than accessioning. Embedding? Cutting? Staining? Where is the CAP line on what is technical and what is non-tecnical? I am not fluent in CAP and would like to know what you all think. Thanks, Matt Lunetta BS HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Non-Certified Histo Aide
Hey Histo Netters, For the CAP pro's; I have been wondering what duties can a non-certified histo-aide preform in a CAP facility? Other than accessioning. Embedding? Cutting? Staining? Where is the CAP line on what is technical and what is non-tecnical? I am not fluent in CAP and would like to know what you all think. Thanks, Matt Lunetta BS HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Chemical Recycling - B/R Instruments(Davide/recycling solvents
We have been running the same B/R Instument for alcohol and xylene for the last 7-8 years. It is fantastic. We have not found any heat issues with it at all, It is run every day ussually two to three runs per day. Matt Lunetta BS HT(ASCP) Longmont United Hospital Message: 2 Date: Tue, 22 Nov 2011 18:05:33 + From: Nancy Schmitt Subject: [Histonet] Chemical Recycling - B/R Instruments(Davide Costanzo) /recycling solvents To: histonet@lists.utsouthwestern.edu Message-ID: 906b4da90ed1db4db6c7e94d7cee6c367b9...@peitha.wad.pa-ucl.com Content-Type: text/plain; charset=us-ascii We use the CBG recycling system - we have 1 for formalin and 1 for alcohol and xylene. They are workhorses and we have had very little issue with them. Our solvent recycler is run every day - we rotate a week at a time between xylene and alcohol. Can anyone provide me information on B/R Instruments Pro series recyclers? I am considerning them vs. CBG Biotech. I have heard rumors that the B/R units throw a lot of heat from the lower boiler and damage the underlying flooring. Can anyone confirm this, or any other troubles with their units? Thank you What is the general opinion about recycling ethanol and xylen? Experiences? Reasonable volumes? NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Audible Alarm
Scott, Yes our processor's are hooked up to an alarm (VIP V and VI) in the Clinical part of the lab with our home phone numbers for them to call. What we ned to watch is when a clinical person turns off the alarm. Ihave heard it is possible to have th alarm also hooked into a pager system. hope this helps, Matt Lunetta BS HT (ASCP) Longmont United Hospital From: Scott, Allison D Subject: [Histonet] Audible alarm To: Message-ID: 1872b4a455b7974391609ad8034c79fc026df...@lbexch01.hchd.local Content-Type: text/plain; charset=us-ascii Hello to all in histoland. We recently had a issue withour tissue processor in regards to a power outage. Once the popwer came back on the processor was in a power out stage and did not process our blocks. Does any one have an external alarm attached to their tissue processor that would alarm in another area when a problem occurs or a automatic dialer that would start calling people. We are using a UPS back up power box on both of our processors right now. I'm trying to see which would be the best way to resolve this issue. Any help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 713-566-5287 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin
You might be surprised I too agree with Joyce and Richard. I understand very well that a new graduate will not be up to the skill level of an individual that has been working for a while. What I am surprised about is that this program seemed to teach to pass the test and has left all of the technical skills left to be taught by the persons 1st job. This could lead to several painful experiences for not only the facility but the new HT. Were is the disconnect. If a person is doing the OJT route they need to have at least one year of experience signed-off by a pathologist. If a person goes through a program who is responsible for making sure that the base-skills are there? Is there not some standards that a new graduate should be able to cut/embed so many blocks in an hour? Is it reasonable for a new graduate to take 1.5 hours to cut 5 (uterus, appendix, tonsil) one-cuts? From facing to lifting the slide off the water-bath? Or to take 2hrs to embed 15 (large tissue sections) one-cut blocks? So Richard is also right how is a new grad to get experiance without that 1st gig? But how much resposiblity should be placed on the 1st gig to train a new HT that is supose to have base skills in lab equipment, cutting, embeding, staining etal? More thoughts ciao Matt Lunetta BS HT(ASCP) Edwards, Richard E. r...@leicester.ac.uk If you do not employ recently trained individuals, how on earth are they going to obtain the experience that Matt craves, it's beyond me. My daughter recently qualified as an Occupational Therapist, when she asked for the reason that she was unsuccessful in obtaining a post, they said that as a recently graduated student she had no experience, what rubbish!!.Thankfully she has now obtained a position under more enlightened management that is to be found here!. Cheers Richard Edwards Shirley A. Powell 08/30/11 6:32 PM I second that Joyce. sp From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce [jwe...@sjha.org] Sent: Tuesday, August 30, 2011 6:17 PM To: Matthew Lunetta; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin I don't understand how a student of any program would have not a portion of their program dedicated to these skills. We partner with Darton College and their students to do a certain number of hours for their Clinicals. They know how to do those things, are trained by the clinical coordinator for the program, and are graded on their work. Are they prepared to go into a lab and work like they've done OJT for 1-2 years? Not at all, but they need to be hired with the understanding that they will need time and patience to develop their speed and their skill. My 2 cents... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Matthew Lunetta Sent: Tuesday, August 30, 2011 13:59 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin Hey all, I found Peggy's comments on why the practical was discontinued to be very interesting. Of late I have had some experience with a new HT that graduated from a program and passed the current HT exam. So, as they say in Great Britain, to stick a pin in the ASCP reasons. This new fresh and shiny HT has all the book knowledge we needed them to have. What they did not have was any technical skills. 1) never used a microscope or centrifuge. 2) no special staining experience 3) no embedding experience 4) no cutting experience When they cut or embed they are no were near the speed, accuracy or quality that is nee ded in our industry. While they can answer any question you ask them they just do not have the technical skills one would expect from a new graduate. I have learned several lessons from this experience. 1) I am so very glad I was one of the last HT's to have taken the practical 2) Any new HT's will be taking a practical if I am involved in the selection process. 3) I will question they quality of any new HT from this particular program While I am sure that there are many new HT's that do have the skills needed, this one experience has caused me to be more cautious. Respectfully, Matt Lunetta BS, HT (ASCP) Message: 2 Date: Tue, 30 Aug 2011 18:09:46 +0200 From: Gudrun Lang Subject: AW: [Histonet] Re: peggy wenk comments on HT/HTL practical To: 'Bob Richmond' Cc: histonet@lists.utsouthwestern.edu Message-ID: 8b7976b131854abc8db236fab5026...@dielangs.at Content-Type: text/plain; charset=iso-8859-1 Dear Dr. Richmond Here in Austria we have a job open for a pathologist with 5 years experience. ;) Please, think
[Histonet] Re:peggy wenk comments on HT/HTL practical - To stick a Pin
Hey all, I found Peggy's comments on why the practical was discontinued to be very interesting. Of late I have had some experience with a new HT that graduated from a program and passed the current HT exam. So, as they say in Great Britain, to stick a pin in the ASCP reasons. This new fresh and shiny HT has all the book knowledge we needed them to have. What they did not have was any technical skills. 1) never used a microscope or centrifuge. 2) no special staining experience 3) no embedding experience 4) no cutting experience When they cut or embed they are no were near the speed, accuracy or quality that is needed in our industry. While they can answer any question you ask them they just do not have the technical skills one would expect from a new graduate. I have learned several lessons from this experience. 1) I am so very glad I was one of the last HT's to have taken the practical 2) Any new HT's will be taking a practical if I am involved in the selection process. 3) I will question they quality of any new HT from this particular program While I am sure that there are many new HT's that do have the skills needed, this one experience has caused me to be more cautious. Respectfully, Matt Lunetta BS, HT (ASCP) Message: 2 Date: Tue, 30 Aug 2011 18:09:46 +0200 From: Gudrun Lang Subject: AW: [Histonet] Re: peggy wenk comments on HT/HTL practical To: 'Bob Richmond' Cc: histonet@lists.utsouthwestern.edu Message-ID: 8b7976b131854abc8db236fab5026...@dielangs.at Content-Type: text/plain; charset=iso-8859-1 Dear Dr. Richmond Here in Austria we have a job open for a pathologist with 5 years experience. ;) Please, think it over to come. Lovely mountains, lovely techs... It sounds, like you are from that sort of pathologist techs dream of. Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Bob Richmond Gesendet: Dienstag, 30. August 2011 04:43 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: peggy wenk comments on HT/HTL practical I really appreciate Peggy Wenk's analysis of the practical examination and why it had to be dropped. I never really understood the issue before. I must confess I always enjoyed helping the prospective examinee obtain exactly the right tissue. No, this endometrium is poorly preserved. We'll arrange with surgery for a completely fresh specimen - I'll block it initially for the diagnosis, then we'll fix it overnight and then block it exactly to specifications. - Ick - this one's been curetted - we'll get another one. I'll block the margins of this colon resection specimen, then we'll pin a portion of tissue onto paraffin and fix it flat overnight. Next time I do an autopsy we'll get a lumbar spinal cord in the intact dura. I'll open the dura dorsally and ventrally with iridectomy scissors, then we'll hang it in neutral buffered formalin for two days. Then I'll tie the dura and dependent nerves with a cotton string. When you embed you'll remove the string, taking care that dura and nerves remain in position. After that it's all yours. If it doesn't work the first time, we've got three more levels in the jar. OK, I'm a geek, I'm 72 years old, I got a right. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: RPM on Cytocentrifuge for CSF
We have ours set at 750 RPM for 2 Min. Matt Lunetta BS,HT(ASCP) Longmont United Hospital -- Message: 2 Date: Thu, 21 Jul 2011 18:30:44 + From: Marcia Fisher Subject: [Histonet] RPM on Cytocentrifuge for CSF To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii How long and at what RPM do you process your CSF cytocentrifuge specimens? Thank you. Marcia Fisher Histology Supervisor/Lab Safety Officer El Centro Regional Medical Center 1415 Ross Ave El Centro, CA 92243 760-339-7267 760-482-5365(F) www.ecrmc.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Billing for CD3/20
Hey all, We are looking at doing the double stain CD3/20 and wanted to know how everyone is billing for it. Can we bill for 2 immuno stains? Or do we have to bill for one? What is the proper CPT for doing double or even triple staining? We are a Medicare facility. Thanks Matt Lunetta BS HT(ASCP) Longmont United Hospital Longmont, Colorado ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Colorado HTL Study Partner
Hey all, I am preparing to take the HTL and was hoping to find a study partner in Colorado, Denver/Boulder/Longmont area. Give me a call or e-mail, Thanks Matt Lunetta, BS HT(ASCP) 303-651-5073 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PRN Longmont United Hospital, Colorado
Hey all, We are looking for a PRN HT(ASCP) to help us out for holidays and the unexpected suprises. Go to LUHCARES.ORG to get al the info and to apply. thanks Matt Lunetta HT(ASCP) Longmont United Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: vytec formalin neutralizer
Nirmala We Use Vytec and after consulting with the city's public utility it is ok for us to dispose of the waste with copious amounts of water down the drain. So, I would contact you local water authority and see if you can do the same. Regards, Matt Lunetta BS HT(ASCP)cm Longmont United Hospital Longmont, Colorado Message: 4 Date: Tue, 22 Mar 2011 10:33:12 -0700 From: sris...@mail.holyname.org Subject: [Histonet] vytec formalin neutralizer To: histonet-boun...@lists.utsouthwestern.edu, Message-ID: Content-Type: text/plain; charset=US-ASCII Hi Everyone, Is there any one out there who is neutralizing the 10% formalin with Vytec neutralizer? How are you disposing the formalin after neutralization? Need some information regarding this. Thanks in advance Nirmala ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PRN in Longmont Colorado
Hey all. We have a PRN position open for an HT here at Longmont United Hospital. Go to luhcares.org to check it out. Thanks Matt Lunetta, HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PRN Position in Longmont Colorado
Hey all, There is a PRN position at Longmont United Hospital open. Working Mon-friday no weekends no evenings. I have attached the link to apply. http://longmontunitedhospital.force.com/Careers/ts2__JobDetails?jobId=a0IA002ct4yMAAtSource= thanks Matt Lunetta HT(ASCP) Longmont United Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Colorado Sate Histo Meeting, April 29-30, 2011
Hi, All are welcome. It is a great program this year. Attached is the program for the 2011 CSH meeting, which will be held April 29th 30th at the Embassy Suites hotel in Loveland, CO. Online registration and credit card payment is available on the CSH website at http://www.coloradohisto.org/2011/meeting.htm Ciao Matt Lunetta HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CAP ANP.22760
hey All, My question is directed to those Hospital labs that have 1(one) DAKO instrument. 1) How are you handling the 'parallel' of this question? 2) What is your validation of the visualization system protocol? 3) How are you running your validation of anti-bodies? We have a few protocols that we have been trying and want to see what others have come up with. Thanks, Matt Lunetta HT(ASCP) Longmont United Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] decalcifying bone marrows after processing
We have excellent sucess in decalcifying the bone prior to processing. We make sure the core has been in fixative for 2 hrs prior to the 20 mins in the decal solution (DeCal STAT). It is then rinced in water and placed in line for standard processing. Matt Lunetta HT (ASCP) Longmont United Hospital Message: 3 Date: Sat, 15 Jan 2011 19:16:14 -0500 From: Richard Cartun Subject: Re: [Histonet] RE: decalcifying bone marrows after processing To: ,, Message-ID: 4d31f280027700020...@gwmail1.harthosp.org Content-Type: text/plain; charset=US-ASCII Hi Becky: How long is the specimen in formalin before you put it in Cal Rite decal? Thanks. Richard Richard W. Cartun, Ph.D. Director, Histology Immunopathology Director, Biospecimens Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Garrison, Becky 01/14/11 5:44 PM We process bone marrow biopsies the same day received. We use a combination decal/ fixative solution (Cal Rite decal) with good results. We keep in this solution a minimum of 3 hours before moving to tissue processor where first 2 solutions are a total of 3 hours in formalin. Although most bone marrow biopsies are received by 2:00 - 3:00pm; we've had some received as late as 4:00pm with good results. Our clinicians place the bone marrow core in pre-filled formalin at the point of collection, also. The Cal Rite is a combination of formaldehyde, formic acid and methanol. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Powers, Kerry Sent: Friday, January 14, 2011 4:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] decalcifying bone marrows after processing I was wondering if anyone has any experience with, or is it even possible to, decalcify bone marrows after they are processed. Our pathologist would like to be able to process bone marrows the same day we receive them, but most of the time there just isn't enough time to allow for proper fixation and then proper decalcification. She asked if we could process them and then decalcify and I have yet to find an answer to this question. Please help!! Thank you, Kerry Powers Comanche Country Memorial Hospital Department of Pathology 3401 W Gore, Lawton OK 73505 (580) 355-8699 ext. 3359 Fax: (580) 585-5462 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Manual Copper stain
A. EQUIPMENT · Coplin Jars · Graduated Cylinders B. REAGENTS RHODANINE SATURATED SOLUTION (stock) p-dimethylaminobenxalrhodanine .3 gm Absolute alcohol 100 ml Let the solution sit to allow sediment to fall to the bottom. RHODANINE SATURATED SOLUTION (working) Rhodanine saturated solution 3 ml Distilled water 40 ml Do not shake stock solution before removing the needed quantity from the top of the solution. Mix the working solution when placing the slides into the coplin jar. DILUTED MAYERS HEMATOXYLIN SOLUTION Mayer*s hematoxylin distilled water (50:50) 0.5% SODIUM BORATE (borax) AQUEOUS Sodium borate 0.5 gm Distilled water 100 ml C. TISSUE CONTROL Tissue positive for Copper 1. Deparaffinize and hydrate to water 2. Incubate in Rhodanine working solution at 67*C for 1 hour. 3. Wash well in several changes of distilled water. 4. Stain in diluted Mayers hematoxylin for 10 minutes. 5. Rinse with distilled water. 6. Quickly rinse in 0.5% sodium borate. 7. Rinse well with distilled water. 8. Dehydrate, clear and mount with synthetic media D. RESULTS Copper * Bright red to red yellow Nuclei * Light blue REFERENCE Modified for altitude from the Rhodanine Method for Copper: Theory and Practice of Histology Sheehan, Hrapchak, Second Edition, pg 230. Hope this helps, Matt Lunetta BS HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Problem with liver fixation
Itai, Are you post fixing in Bouins? Post fix in heated Bouins for 1hr, wash well and then follow your protocal. Hope this helps, Matt Lunetta HT (ASCP) Longmont United Hospital Message: 2 Date: Wed, 15 Sep 2010 18:27:29 +0200 From: Itai Moshe Subject: [Histonet] Re: Problem with liver fixation To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Histonet's I'm using Masson's trichrome to stain paraffin mice diaphragm fixed with PFA. I'm using this protocol: http://www.bcm.edu/mcb/rosenlab/index.cfm?pmid=12997 with sigma masson's kit (#HT15) and Weigert's Iron Hematoxylin Set (#HT1079) The staining is beautiful, but i can't see the nuclei good enough. 1) Is there a way to enhance the nuclei staining ? (the nuclei is the only reason that im not using the simpler sirius red and fast green staining.) 2) What is the meaning for washing in running tap water washing, is it done by putting the slides in a jar with simple tap water for a few minutes ? Thank's Itai ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sakura VIP 6 vs Thermo Scientific Excelsior
Hello fellow Tech's I know this is a repeat posting. I wanted to post again to get responces from some fellow tech's that might have missed the last one. We are looking at getting a new procesor and wanted to get our peers opinons on the Sakura vs Richard Allen. If you are a vendor please do not call or e-mail about processors used or otherwise. The last time I had to feild several calls and we do not want a sales pitch. Thanks, Matt HT (ASCP) Longmont United Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Excelsior Vs. VIP 6
Hello to the World Wide Histo's, We are looking at getting a new processor and was wondering what everyones feelings were on the Excelsior from Thermo vs the VIP 6 from Sakura. We have a VIP5 right now and it is a delight. The demo of the Excelsior was very impressive. Thanks for your thoughts. Matt HT (ASCP) Longmont United Hospital Longmont, Colorado ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Excelsior Vs VIP 6
Hey Histo gang, We are looking for opinions not sales calls. So please do not pass this onto vendors and give us your opinon so we have good feed back from our peers. Thanks, Matt HT ASCP Longmont United Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: FNA stain
Debbie, We use the Diff-Quick stain for immediate evaluation. Matt Lunetta HT (ASCP) Longmont United Hospital Longmont Colorado Message: 2 Date: Wed, 16 Dec 2009 13:24:10 -0500 From: dkb...@chs.net Subject: [Histonet] FNA stain To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII This question is for those of you who perform fine needle aspirations. What stain are you using for your immediate evaluation? Or do you give an immediate evaluation/adequacy? Thanks. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Dako Autostainer
Richard, We have just up-graded to the new Dako Autostainer 48 and after some growing/switching pains (Dako was very responsive in the fixing issues) it is great. The instrument gives very nice consistent results and is also an open system that allows the easy use of other manufactures anti-bodies. If you can call your local rep and get a demo. Matt Lunetta BS HT (ASCP) Longmont United Hospital Colorado Message: 8 Date: Fri, 28 Aug 2009 09:39:59 -0500 (CDT) From: richard...@verizon.net Subject: [Histonet] Dako Autostainer To: histonet@lists.utsouthwestern.edu Message-ID: 1423443284.694606.1251470399584.javamail.r...@vms184.mailsrvcs.net~!B*+R^Content-Type: text/plain; charset=UTF-8 I was wondering if anyone had any comments/concerns about the Dako Autostainer? My lab currently has a Ventana Benchmark LT, but we are looking to switch. Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HT opening in Colorado
Hello Histo-Netters, We have an HT job opening here in Longmont Colorado, located north of Denver and just NE of Boulder. Please apply through the website. I have attached the link. All new and experianced HT's please apply. Also please add my name to your application so we can track the responces from histo net. http://luh.jobscience.com/JsrApp/index.cfm?cmd=showPositionDetailnextEvent=doSearchPositionscobrandId=9000masterId=luh001accountId=9F3D0B3C-E71A-17E7-69B649D49CA1C88FpositionId=511568urlArgList=c2VhcmNoVHlwZT1xdWljayZzdGFydD0xJmNvdW50PTUwJmRlcHRJZD0mam9iSWQ9JmtleXdvcmQ9bid=1297 If the link does not work the web site is www.luhcares.org Thanks Matt Lunetta BS, HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] JOB OPENING IN COLORADO
Hello Histo-Netters, We have an HT job opening here in Longmont Colorado, located north of Denver and just NE of Boulder. Please apply through the website. I have attached the link. All new and experianced HT's please apply. Also please add my name to your application so we can track the responces from histo net. http://luh.jobscience.com/JsrApp/index.cfm?cmd=showPositionDetailnextEvent=doSearchPositionscobrandId=9000masterId=luh001accountId=9F3D0B3C-E71A-17E7-69B649D49CA1C88FpositionId=511568urlArgList=c2VhcmNoVHlwZT1xdWljayZzdGFydD0xJmNvdW50PTUwJmRlcHRJZD0mam9iSWQ9JmtleXdvcmQ9bid=1297 If the link does not work the web site is www.luhcares.org Thanks Matt Lunetta BS, HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology job Opening, Colorado
Hello Histo-Netters, We have an HT job opening here in Longmont Colorado, located north of Denver and just NE of Boulder. Please apply through the website. I have attached the link. All new and experianced HT's please apply. Also please add my name to your application so we can track the responces from histo net. http://luh.jobscience.com/JsrApp/index.cfm?cmd=showPositionDetailnextEvent=doSearchPositionscobrandId=9000masterId=luh001accountId=9F3D0B3C-E71A-17E7-69B649D49CA1C88FpositionId=511568urlArgList=c2VhcmNoVHlwZT1xdWljayZzdGFydD0xJmNvdW50PTUwJmRlcHRJZD0mam9iSWQ9JmtleXdvcmQ9bid=1297 If the link does not work the web site is www.luhcares.org Thanks Matt Lunetta BS, HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: retic stain
I too will send you ours to your e-mail. Matt Lunetta HT (ASCP) Message: 13 Date: Fri, 19 Jun 2009 06:26:34 -0700 (PDT) From: Rene J Buesa rjbu...@yahoo.com Subject: [Histonet] reticulin stain To: histonet@lists.utsouthwestern.edu, Derek Papalegis derek.papale...@tufts.edu Message-ID: 431812.3131...@web65714.mail.ac4.yahoo.com Content-Type: text/plain; charset=iso-8859-1 Derek: I am sending it to you under separate cover. René J. --- On Fri, 6/19/09, Derek Papalegis derek.papale...@tufts.edu wrote: From: Derek Papalegis derek.papale...@tufts.edu Subject: [Histonet] reticulin stain To: histonet@lists.utsouthwestern.edu Date: Friday, June 19, 2009, 9:11 AM I am having some difficulty getting the proper results from my reticulin stain. I have been following and tweaking the procedure outlined in Carson's book without any success. Could someone please forward me their procedure that has all the kinks worked out? Thanks, Derek -- Derek Papalegis HT (ASCP) Senior Histology Technologist Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Dako anti-bodies
Hello Netters We have just changed over to the new DAKO stainer and we have several anti-bodies RTU for the autostainer that we will not be using. All of the have a 2010 experation date. If you are interested in them please contact me off line AE1-3 2010-07 11ml BCL22010-06 11ml CD152009-09 11ml CD3 2010-07 11ml CD302010-01 11ml CD312010-05 7ml CD5 2010-03 11ml CEA 2010-08 11ml CK202010-07 7ml CK7 2010-07 7ml CMV 2010-06 7ml EMA 2010-06 11ml ER 2010-07 11ml ER 2010-07 11ml GFAP2010-02 11ml HMB45 2010-04 11ml HMW 2010-06 11ml Ki672010-08 11ml LCA 2010-06 11ml MELAN A 2009-12 11ml PR 2010-07 11ml PSA 2009-05 11ml S1002010-07 11ml SMA 2010-02 7ml SYNAPTP 2010-02 11ml TTF12010-06 11ml VIMEN 2010-04 11ml thanks, Matt Lunetta HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: specimen tracking from the OR
We too are a small hospital. And like Terri there is a book in the OP area that the specimens are logged into when dropped off and picked up. It is a standard Chain-of-Custody (COC) protocol. We have recently added a new process as we too had a sample missplaced when the Histology Lab was closed. Now after hours tissue is brought to the General lab and a COC log is filled out with the RN dripping off the specimen and the Lab Assistant accepting the specimen. To copy Terri, Crude and time consuming, yesaccurate, too. Regards, Matt Lunetta HT, (ASCP) Longmont United Hospital Message: 2 Date: Mon, 2 Mar 2009 13:28:22 -0500 From: Terri Braud tbr...@holyredeemer.com Subject: [Histonet] RE: specimen tracking from the OR To: histonet@lists.utsouthwestern.edu Message-ID: f3d3b1ce184aa34abb007c3e0fdcc38403848...@hrex-svr.holyredeemer.local Content-Type: text/plain; charset=iso-8859-1 We are a small hospital lab, too. There is a log book in the OR, where specimens are placed for Histo pick up. The circulating nurse puts a Patient sticker and handwrites all specimens removed from the patient, regardless of what they are, and whether or not they have been walked down, tubed, or waiting pickup. We make sure that all specimens we remove are listed in the book, then sign/date/time for them. Any specimen left for us and not in the book, or vice versa, we bring to the attention of the OR control desk. If we make a run and there are no specimens to pick up, then we still sign and date/time the log book as no specimens. Any specimens brought to the department are checked before accepting. Crude and time consuming, yesaccurate, too. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax 6. log book (Kathy Gorham) Message: 6 Date: Sun, 01 Mar 2009 17:15:47 -0800 From: Kathy Gorham gorh...@verizon.net Subject: [Histonet] log book Good Monday Morning, We had a serious incident Friday with O.R. My aide went down to get the specimens from O.R. about 9am. (which were left overs from the night before). She did not stamp in the specimens before she left. When I had time to stamp them in and record them in the log book I discovered that the colon was not there. Two other specimens from that patient where in the bag but no colon. So I went down to O.R. to see where it was. Of course no one knows what happened to the colon. The doctors are furious by all means. Now the O.R. thinks the path lab screwed up. So my questions is how do others log in the specimens as they come into the lab. We have 2 couriers that brings specimens when we are not in the lab from other hospitals. How do you make sure that whom ever brings the specimens actually brings the ones they say they do? Do you have a log book that every specimen that is brought into the lab is written down by the person who brings it in? Right n ow we have a log book but it is written in as we are accessing the specimens. So the specimens may have been there overnight. We are a very small lab and we do almost everything by hand including writing in the log book. Someday we want to be able to scan by bar codes but right now we can not do that. Thanks for any help you can give me. Kathy Gorham, H.T. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Tissue-Tek Prisma Automated slide stainer
Hello, we have been using the Tissue Tek Prisma automated slide stainer and film coverslipper for a few years now and find them to be fantastic. The stainer is very flexable. If you do use the coverslipper make sure you stick with thier film and do not use another brand of film. Matt L Longmont United Hospital -- Message: 8 Date: Tue, 17 Feb 2009 10:53:00 -0600 From: Wilkinson, Joyce E jwilkin...@adclinic.com Subject: [Histonet] Tissue-Tek Prisma Automated slide stainer To: histonet@lists.utsouthwestern.edu Message-ID: aec8fc076dfe3c4eaa91f72ccd1ba7bf01f62...@adcexch1.adc.com Content-Type: text/plain; charset=us-ascii Is anybody using the Tissue Tek Prisma automated slide stainer and film coverslipper? If so, how do you like it. Thanks, Joyce ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: HE Help
You could also try to add some ammonium hydroxide in tap water after the Mayers and before the tap water rinse. This helps us in the bluing on our mayers HE frozen tissue stain line. Add approx 5ml to 150ml of water from 30 sec to 1 min you will have to play with it a little . This blues our tissues up nicely. Matt Lunetta HT Longmont United Hospital Message: 1 Date: Sat, 10 Jan 2009 13:33:09 -0500 From: Weems, Joyce jwe...@sjha.org~!B*+R^Subject: RE: [Histonet] HE help To: lpw...@sbcglobal.net, Charles, Roger rchar...@state.pa.us,~!B*+R^ Histonet histonet@lists.utsouthwestern.edu~!B*+R^Message-ID:~!B*+R^ 5d64396a0d4a5346bebc759022aaeaa5257...@itsssxm01v6.one.ads.che.org~!B*+R^Content-Type: text/plain; charset=us-ascii Also, warm water works best for bluing, if you have that option.. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk Sent: Saturday, January 10, 2009 11:00 AM To: 'Charles, Roger'; 'Histonet' Subject: RE: [Histonet] HE help It might not be a hematoxylin problem. It might be too much eosin. Try one of the following: - cut the eosin time down to 1 minute, and if it's still too pink/not blue enough, cut the eosin time down to 30 seconds. - and/or, change the first 95% alcohol after the eosin to 70% alcohol. That will help to put out more eosin than 95%. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Charles, Roger Sent: Friday, January 09, 2009 9:14 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] HE help Hello All, TGIF I'm trying to help out one of my veterinarian pathologist in getting some information on how to increase the bluing of the HE staining. Presently we are using pre made Mayers Hematoxylin from Sigma with an automated schedule as follows: 1. Drying 45min 2. citrisolve 3 min 3. citrisolve 30 sec 4. citrisolve 3min 5. 100% 45 sec 6. 100% 45 sec 7. 100% 3 min 8. 95% 30 sec 9. 95% 1 min 30 sec 10. Tap water 1 min 11. Hematoxylin 8 min 12. tap water 4 min 13. eosin 1 min 30 sec 14. 95% 1 min 15. 95% 1 min 16. 95% 1 min 17. 100%1 min 18. 100%1 min 19. 100%1 min 20. Citrisolve 1 min 21. Citrisolve 1 min 22. Citrisolve 1 min 23. Citrisolve up to 120 min The pathologist is stating the slides are too eosinic and would like greater bluing. My question is are there limitations to the Mayers Hematoxylin on how blue it will actually get and is there something else we can change in our schedule to increase the bluing or decrease the eosin to get the desired affect? Thanks to all Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 62, Issue 13 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
