[Histonet] Okajima stain control
HI, What would I use for a control for the Okajima stain (hemoglobin). This is my first time doing this stain so any advice would help as well. Thanks! Betsy Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston, TX 77030 832-355-6524 (lab) 832-355-6812 (fax) http://www.texasheart.org Betsy Molinari Senior Histology Research Technician 832-355-6524 | bmolin...@texasheart.orgmailto:bmolin...@texasheart.org | www.texasheart.orghttp://www.texasheart.org 6770 Bertner Ave., MC 1-283, Houston, TX 77030 [Texas Heart Institute]https://secure3.convio.net/thi/site/SPageNavigator/GlobalSiteOptInPage.html[THI News] [THI on Facebook] http://www.facebook.com/Texas.Heart.Institute [THI on Flicker] http://www.flickr.com/photos/texasheart/sets/ [THI on Google] https://plus.google.com/u/0/118043615690351997044/posts [THI on Pinterest] http://pinterest.com/texasheartinst/ [THI on Twitter] http://twitter.com/Texas_Heart [THI on You Tube] http://www.youtube.com/TexasHeartInstitute Confidentiality Notice: This message may be confidential and/or privileged. If you are not the intended recipient you may not review, disseminate or copy this e-mail, its contents and/or any attachments. Please immediately notify the sender If you have received this e-mail in error and delete it from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica 2135 parts
Hi, I am looking for a flex shaft cable for our Leica microtome 2135. I have been told by Leica they no longer service or carry parts for this machine any longer. Ours is in excellent condition and a new one is not in our budget. Is there a Leica “junkyard”? Thanks. Betsy Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston, TX 77030 832-355-6524 (lab) 832-355-6812 (fax) http://www.texasheart.org Betsy Molinari Senior Histology Research Technician 832-355-6524 | bmolin...@texasheart.orgmailto:bmolin...@texasheart.org | www.texasheart.orghttp://www.texasheart.org 6770 Bertner Ave., MC 1-283, Houston, TX 77030 [Texas Heart Institute]https://secure3.convio.net/thi/site/SPageNavigator/GlobalSiteOptInPage.html[THI News] [THI on Facebook] http://www.facebook.com/Texas.Heart.Institute [THI on Flicker] http://www.flickr.com/photos/texasheart/sets/ [THI on Google] https://plus.google.com/u/0/118043615690351997044/posts [THI on Pinterest] http://pinterest.com/texasheartinst/ [THI on Twitter] http://twitter.com/Texas_Heart [THI on You Tube] http://www.youtube.com/TexasHeartInstitute Confidentiality Notice: This message may be confidential and/or privileged. If you are not the intended recipient you may not review, disseminate or copy this e-mail, its contents and/or any attachments. Please immediately notify the sender If you have received this e-mail in error and delete it from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica part, thank you
Thank you for the responses. You have given me lots of leads. Sincerely, Betsy http://www.texasheart.org Betsy Molinari Senior Histology Research Technician 832-355-6524 | bmolin...@texasheart.orgmailto:bmolin...@texasheart.org | www.texasheart.orghttp://www.texasheart.org 6770 Bertner Ave., MC 1-283, Houston, TX 77030 [Texas Heart Institute]https://secure3.convio.net/thi/site/SPageNavigator/GlobalSiteOptInPage.html[THI News] [THI on Facebook] http://www.facebook.com/Texas.Heart.Institute [THI on Flicker] http://www.flickr.com/photos/texasheart/sets/ [THI on Google] https://plus.google.com/u/0/118043615690351997044/posts [THI on Pinterest] http://pinterest.com/texasheartinst/ [THI on Twitter] http://twitter.com/Texas_Heart [THI on You Tube] http://www.youtube.com/TexasHeartInstitute Confidentiality Notice: This message may be confidential and/or privileged. If you are not the intended recipient you may not review, disseminate or copy this e-mail, its contents and/or any attachments. Please immediately notify the sender If you have received this e-mail in error and delete it from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Karnovsky's
Rene, Thank you for your response. It is a whole heart. We have documented the arrival condition and took pictures. I took a small piece and processed it last night and will be cutting it in a few minutes. I am pretty sure the results will be sub-optimal. This will be for documentation. Thanks again, Betsy http://www.texasheart.org Betsy Molinari Senior Histology Research Technician 832-355-6524 | bmolin...@texasheart.orgmailto:bmolin...@texasheart.org | www.texasheart.orghttp://www.texasheart.org 6770 Bertner Ave., MC 1-283, Houston, TX 77030 [Texas Heart Institute]https://secure3.convio.net/thi/site/SPageNavigator/GlobalSiteOptInPage.html[THI News] [THI on Facebook] http://www.facebook.com/Texas.Heart.Institute [THI on Flicker] http://www.flickr.com/photos/texasheart/sets/ [THI on Google] https://plus.google.com/u/0/118043615690351997044/posts [THI on Pinterest] http://pinterest.com/texasheartinst/ [THI on Twitter] http://twitter.com/Texas_Heart [THI on You Tube] http://www.youtube.com/TexasHeartInstitute Confidentiality Notice: This message may be confidential and/or privileged. If you are not the intended recipient you may not review, disseminate or copy this e-mail, its contents and/or any attachments. Please immediately notify the sender If you have received this e-mail in error and delete it from your computer system. From: Rene J Buesa [mailto:rjbu...@yahoo.com] Sent: Wednesday, June 19, 2013 1:21 PM To: Molinari, Betsy; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Karnovsky's Are you referring to a whole heart? If that is the case I think the fixation of the tissue will be very poor because Karnovsky's penetrate very slowly. I do not think it will be worth the effort and materials to process this tissue. René J. From: Molinari, Betsy bmolin...@texasheart.orgmailto:bmolin...@texasheart.org To: Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu Sent: Wednesday, June 19, 2013 1:32 PM Subject: [Histonet] Karnovsky's Hi, I received a heart that was fixed in 2% Karnovsky’s. I was told it was initially kept under refrigeration but then stored at RT. I do not know how long the heart has been stored at RT. By the look of the tissue I would say quite a while. Is this tissue compromised in any way? Should I remove it from the Karnovsky’s and store in in something else? This heart is to undergo paraffin processing. Thanks for any input. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030 832-355-6524 (lab) 832-355-6812 (fax) http://www.texasheart.org Betsy Molinari Senior Histology Research Technician 832-355-6524 | bmolin...@texasheart.orgmailto:bmolin...@texasheart.orgmailto:bmolin...@texasheart.orgmailto:bmolin...@texasheart.org | www.texasheart.orghttp://www.texasheart.org/ 6770 Bertner Ave., MC 1-283, Houston, TX 77030 [Texas Heart Institute]https://secure3.convio.net/thi/site/SPageNavigator/GlobalSiteOptInPage.html[THI News] [THI on Facebook] http://www.facebook.com/Texas.Heart.Institute [THI on Flicker] http://www.flickr.com/photos/texasheart/sets/ [THI on Google] https://plus.google.com/u/0/118043615690351997044/posts [THI on Pinterest] http://pinterest.com/texasheartinst/ [THI on Twitter] http://twitter.com/Texas_Heart [THI on You Tube] http://www.youtube.com/TexasHeartInstitute Confidentiality Notice: This message may be confidential and/or privileged. If you are not the intended recipient you may not review, disseminate or copy this e-mail, its contents and/or any attachments. Please immediately notify the sender If you have received this e-mail in error and delete it from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Karnovsky's
Hi, I received a heart that was fixed in 2% Karnovsky’s. I was told it was initially kept under refrigeration but then stored at RT. I do not know how long the heart has been stored at RT. By the look of the tissue I would say quite a while. Is this tissue compromised in any way? Should I remove it from the Karnovsky’s and store in in something else? This heart is to undergo paraffin processing. Thanks for any input. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030 832-355-6524 (lab) 832-355-6812 (fax) http://www.texasheart.org Betsy Molinari Senior Histology Research Technician 832-355-6524 | bmolin...@texasheart.orgmailto:bmolin...@texasheart.org | www.texasheart.orghttp://www.texasheart.org 6770 Bertner Ave., MC 1-283, Houston, TX 77030 [Texas Heart Institute]https://secure3.convio.net/thi/site/SPageNavigator/GlobalSiteOptInPage.html[THI News] [THI on Facebook] http://www.facebook.com/Texas.Heart.Institute [THI on Flicker] http://www.flickr.com/photos/texasheart/sets/ [THI on Google] https://plus.google.com/u/0/118043615690351997044/posts [THI on Pinterest] http://pinterest.com/texasheartinst/ [THI on Twitter] http://twitter.com/Texas_Heart [THI on You Tube] http://www.youtube.com/TexasHeartInstitute Confidentiality Notice: This message may be confidential and/or privileged. If you are not the intended recipient you may not review, disseminate or copy this e-mail, its contents and/or any attachments. Please immediately notify the sender If you have received this e-mail in error and delete it from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Movats problem solved
Good Friday to all. Histology 101. The Alcian Blue dye itself had lost its efficacy. I borrowed a solution from another lab and voila, perfect staining. Thank you to everyone for their suggestions. I will certainly keep them on file in the event this ever happens again. Have a good weekend. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 1101 Bates St. Houston,TX 77030 832-355-6524 (lab) 832-355-6812 (fax) http://www.texasheart.org Betsy Molinari Senior Histology Research Technician 832-355-6524 | bmolin...@texasheart.orgmailto:bmolin...@texasheart.org | www.texasheart.orghttp://www.texasheart.org 6770 Bertner Ave., MC 1-283, Houston, TX 77030 [Texas Heart Institute][THI News]https://secure3.convio.net/thi/site/SPageNavigator/GlobalSiteOptInPage.html [THI on Facebook] http://www.facebook.com/Texas.Heart.Institute [THI on Flicker] http://www.flickr.com/photos/texasheart/sets/ [THI on Google] https://plus.google.com/u/0/118043615690351997044/posts [THI on Pinterest] http://pinterest.com/texasheartinst/ [THI on Twitter] http://twitter.com/Texas_Heart [THI on You Tube] http://www.youtube.com/TexasHeartInstitute Confidentiality Notice: This message may be confidential and/or privileged. If you are not the intended recipient you may not review, disseminate or copy this e-mail, its contents and/or any attachments. Please immediately notify the sender If you have received this e-mail in error and delete it from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] another Movats question (paraffin)
Hi Peggy, No, this is a batch I have been using since 12/12. I purchased it from Sigma. It seems faint after the ammonium hydroxide . I did a run yesterday and I did get some blue, but faintly, not as bright as I am used to seeing. Everything else was picture perfect. Ray suggested that it may be a change in my tap water. I have our current report and am waiting on the report before that one. I am doing another run today and am considering rinsing in DH2O after the Alcian Blue and ammonium hydroxide. What do you think? Betsy - Betsy Molinari Senior Histology Research Technician 832-355-6524 | bmolin...@texasheart.org | www.texasheart.org 6770 Bertner Ave., MC 1-283, Houston, TX 77030 Confidentiality Notice: This message may be confidential and/or privileged. If you are not the intended recipient you may not review, disseminate or copy this e-mail, its contents and/or any attachments. Please immediately notify the sender If you have received this e-mail in error and delete it from your computer system. - -Original Message- From: Lee Peggy Wenk [mailto:lpw...@sbcglobal.net] Sent: Tuesday, May 28, 2013 6:40 PM To: Molinari, Betsy Subject: Re: [Histonet] another Movats question (paraffin) Any chance this is a new batch of alcian blue? From where did you purchase it? I have a theory, but would like some more information first. Peggy A. Wenk, HTL(ASCP)SLS Lee Peggy Wenk -Original Message- From: Molinari, Betsy Sent: Tuesday, May 28, 2013 11:47 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] another Movats question (paraffin) Hi, The Alcian Blue in my Movats is very faint or seems to completely disappear. I have been running Movats for years and this is a new problem. I have checked lot #’s everything is the same. The solution is 1% pH 2.7. The slides are in solution for 20 mins, rinse for 5 mins in tap H2O, then in alkaline alcohol (90 ml 95% 10 ml ammonium hydroxide) for 1 hr then rinsed for 10 mins. But after hematoxylin the blue is either gone or very faint. Any suggestions? Thanks, Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston , TX 77030 832-355-6524 (lab) 832-355-6812 (fax) http://www.texasheart.org Betsy Molinari Senior Histology Research Technician 832-355-6524 | bmolin...@texasheart.orgmailto:bmolin...@texasheart.org | www.texasheart.orghttp://www.texasheart.org 6770 Bertner Ave., MC 1-283, Houston, TX 77030 [Texas Heart Institute][THI News]https://secure3.convio.net/thi/site/SPageNavigator/GlobalSiteOptInPage.html [THI on Facebook] http://www.facebook.com/Texas.Heart.Institute [THI on Flicker] http://www.flickr.com/photos/texasheart/sets/ [THI on Google] https://plus.google.com/u/0/118043615690351997044/posts [THI on Pinterest] http://pinterest.com/texasheartinst/ [THI on Twitter] http://twitter.com/Texas_Heart [THI on You Tube] http://www.youtube.com/TexasHeartInstitute Confidentiality Notice: This message may be confidential and/or privileged. If you are not the intended recipient you may not review, disseminate or copy this e-mail, its contents and/or any attachments. Please immediately notify the sender If you have received this e-mail in error and delete it from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] another Movats question (paraffin)
Hi, The Alcian Blue in my Movats is very faint or seems to completely disappear. I have been running Movats for years and this is a new problem. I have checked lot #’s everything is the same. The solution is 1% pH 2.7. The slides are in solution for 20 mins, rinse for 5 mins in tap H2O, then in alkaline alcohol (90 ml 95% 10 ml ammonium hydroxide) for 1 hr then rinsed for 10 mins. But after hematoxylin the blue is either gone or very faint. Any suggestions? Thanks, Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston , TX 77030 832-355-6524 (lab) 832-355-6812 (fax) http://www.texasheart.org Betsy Molinari Senior Histology Research Technician 832-355-6524 | bmolin...@texasheart.orgmailto:bmolin...@texasheart.org | www.texasheart.orghttp://www.texasheart.org 6770 Bertner Ave., MC 1-283, Houston, TX 77030 [Texas Heart Institute][THI News]https://secure3.convio.net/thi/site/SPageNavigator/GlobalSiteOptInPage.html [THI on Facebook] http://www.facebook.com/Texas.Heart.Institute [THI on Flicker] http://www.flickr.com/photos/texasheart/sets/ [THI on Google] https://plus.google.com/u/0/118043615690351997044/posts [THI on Pinterest] http://pinterest.com/texasheartinst/ [THI on Twitter] http://twitter.com/Texas_Heart [THI on You Tube] http://www.youtube.com/TexasHeartInstitute Confidentiality Notice: This message may be confidential and/or privileged. If you are not the intended recipient you may not review, disseminate or copy this e-mail, its contents and/or any attachments. Please immediately notify the sender If you have received this e-mail in error and delete it from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: another Movats question (paraffin)
Donella, I use Verhoffs. - Betsy Molinari Senior Histology Research Technician 832-355-6524 | bmolin...@texasheart.org | www.texasheart.org 6770 Bertner Ave., MC 1-283, Houston, TX 77030 Confidentiality Notice: This message may be confidential and/or privileged. If you are not the intended recipient you may not review, disseminate or copy this e-mail, its contents and/or any attachments. Please immediately notify the sender If you have received this e-mail in error and delete it from your computer system. - -Original Message- From: Donella Stillings [mailto:dstilli...@unipathdx.com] Sent: Tuesday, May 28, 2013 10:56 AM To: Molinari, Betsy Subject: RE: another Movats question (paraffin) Are you using Weigert's Hematoxylin or regular Hematoxylin? Donella -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Tuesday, May 28, 2013 9:47 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] another Movats question (paraffin) Hi, The Alcian Blue in my Movats is very faint or seems to completely disappear. I have been running Movats for years and this is a new problem. I have checked lot #’s everything is the same. The solution is 1% pH 2.7. The slides are in solution for 20 mins, rinse for 5 mins in tap H2O, then in alkaline alcohol (90 ml 95% 10 ml ammonium hydroxide) for 1 hr then rinsed for 10 mins. But after hematoxylin the blue is either gone or very faint. Any suggestions? Thanks, Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston , TX 77030 832-355-6524 (lab) 832-355-6812 (fax) http://www.texasheart.org Betsy Molinari Senior Histology Research Technician 832-355-6524 | bmolin...@texasheart.orgmailto:bmolin...@texasheart.org | www.texasheart.orghttp://www.texasheart.org 6770 Bertner Ave., MC 1-283, Houston, TX 77030 [Texas Heart Institute][THI News]https://secure3.convio.net/thi/site/SPageNavigator/GlobalSiteOptInPage.html [THI on Facebook] http://www.facebook.com/Texas.Heart.Institute [THI on Flicker] http://www.flickr.com/photos/texasheart/sets/ [THI on Google] https://plus.google.com/u/0/118043615690351997044/posts [THI on Pinterest] http://pinterest.com/texasheartinst/ [THI on Twitter] http://twitter.com/Texas_Heart [THI on You Tube] http://www.youtube.com/TexasHeartInstitute Confidentiality Notice: This message may be confidential and/or privileged. If you are not the intended recipient you may not review, disseminate or copy this e-mail, its contents and/or any attachments. Please immediately notify the sender If you have received this e-mail in error and delete it from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] harder paraffin
Hi Histonetters, Good Friday to you all. Do you know if there is a “harder” paraffin that may offer more support to the tissue while cutting? I currently use Paraplast Plus and love it, but now I am cutting some polymer material and there is some tearing and was thinking that maybe a different paraffin would help. I am trying to avoid embedding in plastic. Thanks, Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 1101 Bates Street Houston,TX 77030 832-355-6524 (lab) 832-355-6812 (fax) [THI Celebrates 50 Years]http://www.texasheart.org/AboutUs/History/index.cfm Betsy Molinari Senior Histology Research Technician 832-355-6524 | bmolin...@texasheart.orgmailto:bmolin...@texasheart.org www.texasheart.orgwww.texasheart.orghttp://www.texasheart.org TEXAS HEART INSTITUTE 6770 Bertner Ave. MC 1-283 | Houston, TX 77030 [Receive electronic news from THI]https://secure3.convio.net/thi/site/SPageNavigator/GlobalSiteOptInPage.html[THI on Facebook]http://www.facebook.com/Texas.Heart.Institute[THI on Twitter]http://twitter.com/Texas_Heart[THI on YouTube]http://www.youtube.com/TexasHeartInstitute[THI's Flickr page]http://www.flickr.com/photos/texasheart/sets/ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient, or an authorized representative of the intended recipient, you are hereby notified that any review or dissemination or copying of this e-mail and its attachments, if any, or the information contained herein, is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Verhoeff's with a green counterstain
Hi, I have not heard of this stain before, but saw mention of it in a paper. I could not find a protocol. Could someone tell me more about it? Thanks! Betsy Molinart HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houaton, TX 77030 832-355-6524 (lab) 832-355-6812 (fax) [THI Celebrates 50 Years]http://www.texasheart.org/AboutUs/History/index.cfm Betsy Molinari Senior Histology Research Technician 832-355-6524 | bmolin...@texasheart.orgmailto:bmolin...@texasheart.org www.texasheart.orgwww.texasheart.orghttp://www.texasheart.org TEXAS HEART INSTITUTE 6770 Bertner Ave. MC 1-283 | Houston, TX 77030 [Receive electronic news from THI]https://secure3.convio.net/thi/site/SPageNavigator/GlobalSiteOptInPage.html[THI on Facebook]http://www.facebook.com/Texas.Heart.Institute[THI on Twitter]http://twitter.com/Texas_Heart[THI on YouTube]http://www.youtube.com/TexasHeartInstitute[THI's Flickr page]http://www.flickr.com/photos/texasheart/sets/ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient, or an authorized representative of the intended recipient, you are hereby notified that any review or dissemination or copying of this e-mail and its attachments, if any, or the information contained herein, is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Zinc-Tris mouse hearts
Hi, I am going to have some mouse hearts that have been fixed in zinc-Tris fixative. I have never worked with this before. My question is about processing. After doing some looking, I found an article that advised checking the solutions on the processor , the first couple of alcohols , to be sure there is no carry over of formalin. I understand that the object if this fixative is to avoid formalin but is this step necessary? Thank you in advance. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC 1-283 Houston, TX 77030 832-355-6524 (lab) 832-355-6812 (fax) [THI Celebrates 50 Years]http://www.texasheart.org/AboutUs/History/index.cfm Betsy Molinari Senior Histology Research Technician 832-355-6524 | bmolin...@texasheart.orgmailto:bmolin...@texasheart.org www.texasheart.orgwww.texasheart.orghttp://www.texasheart.org TEXAS HEART INSTITUTE 6770 Bertner Ave. MC 1-283 | Houston, TX 77030 [Receive electronic news from THI]https://secure3.convio.net/thi/site/SPageNavigator/GlobalSiteOptInPage.html[THI on Facebook]http://www.facebook.com/Texas.Heart.Institute[THI on Twitter]http://twitter.com/Texas_Heart[THI on YouTube]http://www.youtube.com/TexasHeartInstitute[THI's Flickr page]http://www.flickr.com/photos/texasheart/sets/ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient, or an authorized representative of the intended recipient, you are hereby notified that any review or dissemination or copying of this e-mail and its attachments, if any, or the information contained herein, is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] calcium buildup
Hi, Does anyone know of a way to remove calcium buildup on the wash nozzles in the water reservoirs. I have the Tissue-Tek DRS 2000. Thank you. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston,TX 77030 832-355-6524 832-355-6812 (fax) [THI Celebrates 50 Years]http://www.texasheart.org/AboutUs/History/index.cfm Betsy Molinari Senior Histology Research Technician 832-355-6524 | bmolin...@texasheart.orgmailto:bmolin...@texasheart.org www.texasheart.orgwww.texasheart.orghttp://www.texasheart.org TEXAS HEART INSTITUTE 6770 Bertner Ave. MC 1-283 | Houston, TX 77030 [Receive electronic news from THI]https://secure3.convio.net/thi/site/SPageNavigator/GlobalSiteOptInPage.html[THI on Facebook]http://www.facebook.com/Texas.Heart.Institute[THI on Twitter]http://twitter.com/Texas_Heart[THI on YouTube]http://www.youtube.com/TexasHeartInstitute[THI's Flickr page]http://www.flickr.com/photos/texasheart/sets/ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient, or an authorized representative of the intended recipient, you are hereby notified that any review or dissemination or copying of this e-mail and its attachments, if any, or the information contained herein, is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: bone regrowth
From: Molinari, Betsy Sent: Thursday, June 28, 2012 7:20 AM To: histonet@lists.utsouthwestern.edu Subject: bone regrowth Hi all, I have a couple of questions regarding decal and staining. I have done some searching but could use some hands on experiences. It involves bone regrowth. The sample will vary in size. From my reading, it seems that 5% formic or 10% EDTA is preferred. The researcher asked about acetic acid, but I have never heard as acetic alone as a decal, isn’t it just used to balance pH of the EDTA? It is my understanding that the decal step will be done somewhere else then the sample sent to me. That lab feels that HCL decal will not not affect the sample. I think otherwise. The staining they have talked about is the Goldners Trichrome mainly. The researcher mentioned Safranin O but from my conversations I think they have little knowledge of histology and I work in a cardiovascular lab with my only experience has been with mouse tibia and these samples will be from a larger source. Type 2 collagen was brought up but when I said that was an immuno stain they did not realize that. Si in a nutshell, what decal sol. would be better and what stain? Sorry for the rambling. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology [THI Celebrates 50 Years] Betsy Molinari Senior Histology Research Technician 832-355-6524 | bmolin...@texasheart.orgmailto:bmolin...@texasheart.org www.texasheart.orgwww.texasheart.orghttp://www.texasheart.org TEXAS HEART INSTITUTE 6770 Bertner Ave. MC 1-283 | Houston, TX 77030 [Receive electronic news from THI]https://secure3.convio.net/thi/site/SPageNavigator/GlobalSiteOptInPage.html[THI on Facebook]http://www.facebook.com/Texas.Heart.Institute[THI on Twitter]http://twitter.com/Texas_Heart[THI on YouTube]http://www.youtube.com/TexasHeartInstitute[THI's Flickr page]http://www.flickr.com/photos/texasheart/sets/ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient, or an authorized representative of the intended recipient, you are hereby notified that any review or dissemination or copying of this e-mail and its attachments, if any, or the information contained herein, is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Von Kossa vs Alizarin Red
Hi, Under what circumstances would you prefer one over the other? Thanks! Happy Friday! I hope all you convention goers ( is that even a word??) are having a great time! Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] How many tissues an histo tech is suppose to cut per
I once went to an interview at a research lab , will remain unnamed, and they asked if I could cut 300 /hr. I said Is this a trick question? They just stared back. I withdrew my app. LOL Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston, TX 77030 832-355-6524 832-355-6812 (fax) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Tuesday, June 28, 2011 4:27 PM To: 'Markus F. Meyenhofer'; Stella Mireles; Joanne Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut per Perhaps your med techs should try cutting 100 an hour with quality sections that a pathologist can read!!! They are to accustom to high throughput testing with only pushing a button in some cases. I know med tech that are excellent Histologist but they do the work too. Pam Marcum UAMS -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Markus F. Meyenhofer Sent: Tuesday, June 28, 2011 4:22 PM To: Stella Mireles; Joanne Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] How many tissues an histo tech is suppose to cut per Maybe 100 sections from one block??? - Original Message - From: Stella Mireles estellamire...@gmail.com To: Joanne joanne0...@comcast.net Cc: histonet@lists.utsouthwestern.edu Sent: Tuesday, June 28, 2011 4:49 PM Subject: Re: [Histonet] How many tissues an histo tech is suppose to cut per Joanne is not alone. The lab I work at has informed us that due to their own research, a single histotech should be able to do 100 blocks an hour. I'm not sure if they expect embedding, trimming, writing slides, sectioning and manual staining in this goal. I am a seasoned histotech, and have tried to speak to my lab manager and lab supervisor, (both are med. techs and have very little insight into what is involved in producing a high quality slide), but their goal remains the same. I have an idea: I feel like printing some of your responses to Joanne question and showing them that their goal is unattainable as well. Any other suggestions. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] How many tissues an histo tech is suppose to cut per
SorryI meant 300 in a 8hr day..including embedding, etc -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Thursday, June 30, 2011 6:10 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut per I once went to an interview at a research lab , will remain unnamed, and they asked if I could cut 300 /hr. I said Is this a trick question? They just stared back. I withdrew my app. LOL Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston, TX 77030 832-355-6524 832-355-6812 (fax) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Tuesday, June 28, 2011 4:27 PM To: 'Markus F. Meyenhofer'; Stella Mireles; Joanne Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut per Perhaps your med techs should try cutting 100 an hour with quality sections that a pathologist can read!!! They are to accustom to high throughput testing with only pushing a button in some cases. I know med tech that are excellent Histologist but they do the work too. Pam Marcum UAMS -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Markus F. Meyenhofer Sent: Tuesday, June 28, 2011 4:22 PM To: Stella Mireles; Joanne Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] How many tissues an histo tech is suppose to cut per Maybe 100 sections from one block??? - Original Message - From: Stella Mireles estellamire...@gmail.com To: Joanne joanne0...@comcast.net Cc: histonet@lists.utsouthwestern.edu Sent: Tuesday, June 28, 2011 4:49 PM Subject: Re: [Histonet] How many tissues an histo tech is suppose to cut per Joanne is not alone. The lab I work at has informed us that due to their own research, a single histotech should be able to do 100 blocks an hour. I'm not sure if they expect embedding, trimming, writing slides, sectioning and manual staining in this goal. I am a seasoned histotech, and have tried to speak to my lab manager and lab supervisor, (both are med. techs and have very little insight into what is involved in producing a high quality slide), but their goal remains the same. I have an idea: I feel like printing some of your responses to Joanne question and showing them that their goal is unattainable as well. Any other suggestions. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] stainless steel cassettes
Hi , Does anyone have any stainless steel cassettes they would like to sell or does anyone know where we could purchase them? As always, thank you for your help and happy hump day! Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. MC 1-283 Houston, TX 77030 832-355-6524 832-355-6812 (fax) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Bielschowskys Stain
Glawon, Thank you for your help. I appreciate it. Betsy -Original Message- From: Glawon Flood [mailto:smoo...@gmail.com] Sent: Tuesday, March 15, 2011 2:33 PM To: Molinari, Betsy Subject: Re: [Histonet] Bielschowskys Stain Betsy, I don't know of any modifications for Eager's but there are modifications for the Fink-Heimer technique as well as the Nauta-Gygax technique that can be used on paraffin-embedded tissue. I've only come across these in passing and you may have to do a little digging to find a protocol. I hope the info proves useful...good luck! Glawon Flood, HTL (ASCP) Won On Mar 15, 2011, at 11:43 AM, Molinari, Betsy bmolin...@heart.thi.tmc.edu wrote: Thank you for your response. I googled Eagers and found a protocol for Eagers for frozen sections. I have paraffin blocks. Do you know if there is and Eagers method for paraffin? Thank you so much. Betsy -Original Message- From: Glawon Flood [mailto:smoo...@gmail.com] Sent: Tuesday, March 15, 2011 10:22 AM To: Molinari, Betsy Subject: Re: [Histonet] Bielschowskys Stain Hi Betsy, Let me start off by saying that I am by no means an expert on what's preferred. However, in my experience, I've come to find the Bielschowsky method very useful for staining nerve fibers, but I would not consider it so for demonstrating axonal degeneration. I would suggest that you maybe consider Eager's method for degenerating axons. Unlike Bielschowsky's, Eager's actually distinguishes those fibers that are normal from those that are degenerating. I hope this helps. Glawon Flood, HTL (ASCP) On Mar 15, 2011, at 6:00 AM, Molinari, Betsy bmolin...@heart.thi.tmc.edu wrote: Hi , An investigator would like show axonal degeneration in peripheral nerves. Is Bielschowsky's the preferred stain? Thanks! Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. MC 1-283 Houston, TX 77030 832-355-6524 832-355-6812 (fax) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Bielschowskys stain
Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. MC 1-283 Houston, TX 77030 832-355-6524 832-355-6812 (fax) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HE stain uneven
I had this problem awhile back. Did you change your solvent for deparaffinization? I changed from xylene to another solvent and realized I had to extend the time my slides were in it. Betsy Molinari HT(ASCP) Texas Heart Institue Cardiovascular Pathology 1101 Bates Street Houston, TX 77030 832-355-6524 (lab) 832-355-6812 (fax) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Madeline Gi Sent: Friday, February 11, 2011 12:44 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] HE stain uneven Hello everyone in the histology world, I have a problem with my HE stain this just started and I am not sure why. Can someone tell me how does a routine HE stain unevenly? I am currently using Gill III I run it down as usual this routine worked well from months and now it is uneven any suggestion would be greatly appreciated…. Madeline Rotger Milanese H.T. BSHCS 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelin...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] frozen lung sections
Hi all, I have received some lung samples on OCT. These lungs have not been in PBS or any fixative. They went directly from dissection into OCT and frozen. I cannot get a section. The temp of the cryostat is -20. I have done some reading and it seems the best results come from immersion in PBS, then 4% paraformaldehyde then into sucrose. Can anyone clarify the procedure or offer advice? I am unfamiliar with this technique since most of my frozen sectioning is heart tissue and it does not present the same problem as the lung. Thanks, Betsy Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] staining questions
Good morning! I will be staining vessels for proteoglycans using Alcian Blue. I was going to use the 2.5pH protocol with a Nuclear Fast Red counterstain. Does anyone have any other suggestion? The investigator does not want Movats. Also, I normally use PTAH as my fibrin stain for clots but was thinking that there may be something better. I found the Fraser-Lendrum but the protocol stated that Zenkers was the best fixative and that is out of the question. So again, any suggestions? Thank you in advance. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Movats on mouse aortas
Hi, I have had a problem with splotchy elastin staining on certain sections of mouse aorta. I use the Modified Movats and do not use a microwave. But from this one lab some areas of the elastic fibers will retain the hematolylin after differentiation while some areas lose it completely, all in the same section. They fix in 10%NBF and the processing gets done at their lab and I receive the unstained slides. My control does not have this problem. Any ideas? Thanks, Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica RM2165 instruction handbook
Hi, Does anyone have a handbook for this microtome that they would be willing to fax to me? We inherited one with no instruction book. I have searched online and cannot find it. Thanks. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE:Missing the point of Plants-in-the-lab OT
This had nothing to do with an inspection, but follows this comment. There were 3 plants in our lab and there was one histotech who starting sneezing and got congested whenever he sat at his microtome. The plants were in front of him on a raised shelf. Finally we had a thought it may be the plants so we took them down and had a closer look and indeed there was a moldy material growing in the soil. Removed the plants and it was resolved. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston, TX 77030 832-355-6524 832-355-6812 (fax) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Haynes, MaryAnne Sent: Thursday, October 22, 2009 2:35 PM To: Akemi Allison-Tacha; Sara''Breeden; histonet@lists.utsouthwestern.edu; LindaBlazek; Patti Loykasek Subject: RE: [Histonet] RE:Missing the point of Plants-in-the-lab OT Some Health departments state that plants and their potting soil can be a potential microbial and fungi contaminate in the lab. Mary Anne Haynes Mary Anne D. Haynes, MBHA, DLM, SLS(ASCP) Pathology Manager Children's National Medical Center Department of Anatomic Pathology 111 Michigan Ave NW Washington, DC 20010 202-476-4311 (office) 202 476-4030 (fax) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Thursday, October 22, 2009 15:26 To: Sara''Breeden; histonet@lists.utsouthwestern.edu; LindaBlazek; Patti Loykasek Subject: Re: [Histonet] RE:Missing the point of Plants-in-the-lab OT Hi All, I think all of you are missing the point of Patti's question. She stated that her lab was dinged for having plants in the lab by a CAP inspector. I had the same thing happen to me years ago. The inspector stated that plants attract insects that can contaminate a supposedly clean environment. Patti has an extremely well organized lab that only had a small phase (1) deficiency last year. I think the inspector couldn't find anything, so they had to come up with this ridiculous infraction. Akemi Allison-Tacha BS, HT(ASCP)HTL PresidentPhoenix Lab ConsultingTele: 408.402.5257 Cell: 408.335.9994 E-Mail: akemiat3...@yahoo.com --- On Thu, 10/22/09, Blazek, Linda lbla...@digestivespecialists.com wrote: From: Blazek, Linda lbla...@digestivespecialists.com Subject: [Histonet] RE: Plants-in-the-lab OT To: 'Breeden, Sara' sbree...@nmda.nmsu.edu, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Thursday, October 22, 2009, 12:15 PM I don't know Sally, but where I worked many moons ago I had a spider plant that was extremely prolific. The powers that be made me remove it from the lab for an inspection. It went to live in one of the administrator's office for several months. And died! I think it needed the fumes! Or it missed me. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Phone: (937) 293-4424 ext 7118 Email: lbla...@digestivespecialists.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, October 22, 2009 3:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Plants-in-the-lab OT I think it's the fluorescent lights that makes them thrive. The absorption of Fume Matter is a secondary, but beneficial, effect. You go, chlorophyll! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] KP Marker Plus
Mercedes Medical 800-331-2716 Betsy Molinari HT (ASCP) Texas heart Institute Cardiovascular Pathology Houston,TX 77090 832-355-6524 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Metzger, Kenneth Sent: Friday, May 15, 2009 11:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] KP Marker Plus Does anyone know who sells the KP Marker PLUS? I can't seem to find them. Thanks for any help. Ken Kenneth G Metzger HTL(ASCP) ARUP Labs Histology Supervisor 500 Chipeta Way Salt Lake City, Utah 84108-1221 kenneth.metz...@aruplab.com (801) 583-2787 x 3101 - -- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] decolorizing MMA
Good morning, Is it possible to decolorize MMA slides? Thanks, Betsy Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] contrast
Hi, I have some mouse hindlimbs that have catheterized with Omnipaque. The Omnipaque contains 302 ml of iohexol which is equivalent to 140 mg of organic iodine per ml. The mice will be sacrificed immediately after being radiographed. They would like them to be processed in paraffin. I am wondering if this will have any effect on the muscle. Thanks. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: [Histonet] uncertified techs in Histology
-Original Message- From: Molinari, Betsy Sent: Wednesday, February 18, 2009 12:54 PM To: 'Bernice Frederick' Subject: RE: [Histonet] uncertified techs in Histology I don't like the generality of this statement. I work in a research lab and my stuff is not crap. The people that I work with do not produce crap, HT or no HT. I find research more challenging in fact than clinical. Everyday I get animal specimens of all types in various fixatives. Some have device implants of various materials and some are looking for cellular changes. Everything must be of photographic quality. I take great pride in my work and other techs in my department. At times I do receive samples that have been abused by the doctor or research fellow but that is not my labs' fault and we will speak to the researcher about future handling of their samples. Maybe I misunderstood and am reacting too strongly to you statement, but I get tired of some thinking that research histo techs are second class citizens. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston, TX 77030 832-355-6524 832-355-6812 (fax) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Wednesday, February 18, 2009 7:33 AM To: 'JoeNocito'; 'Rittman, Barry R'; 'Histonet' Subject: RE: [Histonet] uncertified techs in Histology And another note- research labs think they can train anyone to do histology (trust me on this) and then can't figure out why their stuff is crap and then try to tell us how to do our job. This just happened and it wasn't the processing (we did it) but the quality of the sample submitted! As we are classified at NU as Sr. Research techs, we have to really fight the U for salary and we are only in the middle of the scale, but we are in a very relaxed atmosphere. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of JoeNocito Sent: Tuesday, February 17, 2009 6:39 PM To: 'Rittman, Barry R'; 'Histonet' Subject: RE: [Histonet] uncertified techs in Histology Don't hold back Barry, tell us what you really think JTT -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: Tuesday, February 17, 2009 4:16 PM To: 'Histonet' Subject: RE: [Histonet] uncertified techs in Histology I think that the entire system needs a good enema. Ever since I started work in a lab in 1957, salaries have been poor and not kept up with cost of living or reflected the skill of many of the histotechs. I feel that one of the major problems is that we have several categories of histotechs. Certified (I mean that in the nicest sense of the word!). Certified and also highly skilled. Skilled via on the job training. Robotic technicians. Until a system is put into place that requires all histotechs to be certified, or in training along a specific career pathway then things will never change. Along with this must be a system that requires employers to have well defined career paths for all their histotechs. This must include training outside of the routine work that they need to do in their specific laboratory. I must agree that getting rid of the practical part of the examination was how can I put this delicately ... imbecilic. The workplace needs to get back to being less of a bottom line factory in which careers of the employees is often not a consideration to a place where career paths are an integral part of the employment. The optimal environment is one where there is a two way street where both employer and employee benefit. While money is a consideration, it is not the only reason that individuals continue to work in a specific lab. Barry -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Judith L. Williams Sent: Tuesday, February 17, 2009 1:36 PM To: Pamela Marcum Cc: 'Histonet'; 'Donna Hunter' Subject: RE: [Histonet] uncertified techs in Histology Hi all in histo world - well, time to get on the bandwagon! so, I agree with the other Old TEchs that came through the hard way like I did- it was much more of a challenge and the certification really meant something you worked HARD for! How do we pass that on the our newer techs. I think it is up to us to make sure they know how to do things- how to troubleshoot- how to make things by hand should they not get in the order of pre-made stuff! In a busy work world it is a challenge- but the only way they will learn now is if we train them. Are the schools doing this? yes, some are. The students that work with someone
RE: [Histonet] uncertified techs in Histology
Thanks for the clarification Bernice! -Original Message- From: Bernice Frederick [mailto:b-freder...@northwestern.edu] Sent: Wednesday, February 18, 2009 1:31 PM To: Molinari, Betsy Subject: RE: [Histonet] uncertified techs in Histology Betsy I was referring to those researchers that bring the crap (non-techs trying to do the histo). Not what we do. I do feel we are second class citizens because it is felt that anyone can do histo. They read a procedure in a paper and think we can do magic or, my favorite, give us IHC conditions that are done by hand and we do it on the autostainer. Sorry if you took it the wrong way. I was not picking on histotechs, but the non-knowledgable researcher that think they are histotechs and know more about it because the might have a PhD. I could get really started on that. I had to explain to someone the difference between a cross, longitudinal and tangential section and this person was supposed to have a science background! Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: Molinari, Betsy [mailto:bmolin...@heart.thi.tmc.edu] Sent: Wednesday, February 18, 2009 12:54 PM To: Bernice Frederick Subject: RE: [Histonet] uncertified techs in Histology I don't like the generality of this statement. I work in a research lab and my stuff is not crap. The people that I work with do not produce crap, HT or no HT. I find research more challenging in fact than clinical. Everyday I get animal specimens of all types in various fixatives. Some have device implants of various materials and some are looking for cellular changes. Everything must be of photographic quality. I take great pride in my work and other techs in my department. At times I do receive samples that have been abused by the doctor or research fellow but that is not my labs' fault and we will speak to the researcher about future handling of their samples. Maybe I misunderstood and am reacting too strongly to you statement, but I get tired of some thinking that research histo techs are second class citizens. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston, TX 77030 832-355-6524 832-355-6812 (fax) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Wednesday, February 18, 2009 7:33 AM To: 'JoeNocito'; 'Rittman, Barry R'; 'Histonet' Subject: RE: [Histonet] uncertified techs in Histology And another note- research labs think they can train anyone to do histology (trust me on this) and then can't figure out why their stuff is crap and then try to tell us how to do our job. This just happened and it wasn't the processing (we did it) but the quality of the sample submitted! As we are classified at NU as Sr. Research techs, we have to really fight the U for salary and we are only in the middle of the scale, but we are in a very relaxed atmosphere. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of JoeNocito Sent: Tuesday, February 17, 2009 6:39 PM To: 'Rittman, Barry R'; 'Histonet' Subject: RE: [Histonet] uncertified techs in Histology Don't hold back Barry, tell us what you really think JTT -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: Tuesday, February 17, 2009 4:16 PM To: 'Histonet' Subject: RE: [Histonet] uncertified techs in Histology I think that the entire system needs a good enema. Ever since I started work in a lab in 1957, salaries have been poor and not kept up with cost of living or reflected the skill of many of the histotechs. I feel that one of the major problems is that we have several categories of histotechs. Certified (I mean that in the nicest sense of the word!). Certified and also highly skilled. Skilled via on the job training. Robotic technicians. Until a system is put into place that requires all histotechs to be certified, or in training along a specific career pathway then things will never change. Along with this must be a system that requires employers to have well defined career paths for all their histotechs. This must include training outside of the routine work that they need to do in their specific laboratory. I must agree that getting rid of the practical part of the examination was how can I put this delicately ... imbecilic. The workplace needs to get back to being less of a bottom line factory in which careers of the employees is often not a consideration to a place where career paths are an integral part
RE: [Histonet] Plastic embedding
You and the rest of the world Armando. Information like this is costly and hard to come by. Best of luck none the less. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston, TX 77225 832-355-6524 832-355-6812 (fax) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Armando Tellez Sent: Wednesday, January 28, 2009 4:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Plastic embedding Hi, Does anyone have a protocol for plastic embedding that shows in detail the procedure and equipment needed for this purpose that does not mind share it? My office email is attached to my signature. Please let me know since my boss want to start a stent pathology protocls and he claims that we have the equipment, so first I need a good full blown descriptive protocol and all the protocols in internet are already assuming that you have some plastic embedding knowledge and I have never embed any metal devices. (I need a protocol for microtome, not grinding) Thanks Armando Tellez atel...@crf.org mailto:atel...@crf.org Pathology Department Tel: (845) 290 8034 Fax: (845) 290 8030 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Coverslippers
I use the Sakura Tissue Tek Glas and love it. Low maintenance, easy to clean and simple to program. From what I understand if you have a high production of slides glass may be too slow . Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Avenue MC1-283 Houston,TX 77030 832-355-6524 832-355-6812 (fax) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Sunday, January 18, 2009 9:44 AM To: histonet@lists.utsouthwestern.edu; Knutson, Deanne Subject: Re: [Histonet] Coverslippers Deanne: Try to get a demo from Sakura. Regarding glass vs. film: FILM: faster and slides can be filed immediately; after YEARS the film may become loose, but only if it was placed with less than needed xylene. GLASS: better for photomicrography IF you use apochromat high power dry objective and glass remains better in the long time. They have a higher failure rate (more interruptions in the work flow). Sometimes slides need to wait flat for days before being filed. I personally prefer film, the net result outweigts its drawbacks. René J. --- On Sat, 1/17/09, Knutson, Deanne dknut...@primecare.org wrote: From: Knutson, Deanne dknut...@primecare.org Subject: [Histonet] Coverslippers To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Date: Saturday, January 17, 2009, 3:47 PM Our lab is interested in purchasing a coverslipping instrument and would like to hear from the rest of you on what type is a good one to purchase? Also, what are the pros and cons on glass coverslips vs plastic coverslips vs film for coverslips. Thank you in advance for sharing your knowledge on this topic. Deanne Knutson HTL,MT (ASCP) Anatomic Pathology Supervisor St. Alexius Medical Center Office: 530-6730 dknut...@primecare.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Paraffin wax
We have used Paraplast Plus for years. For both processing and embedding. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC 1-283 Houston, TX 77030 832-355-6524 832-355-6812 (fax) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Histonet Alias Sent: Thursday, December 11, 2008 9:07 AM To: Charles, Roger Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin wax I have had very good luck with Paraplast Plus for infiltration and using the Paraplast Xtra for embedding. On Thu, Dec 11, 2008 at 10:00 AM, Charles, Roger rchar...@state.pa.uswrote: Hello, Could someone share their knowledge of the Paraplast line of paraffin with me? We are being forced to change from the TissuePrep line. Thanks Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Al Ias HT(ASCP) Histology Manager Pathology Laboratory United States ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] wavy mid myocardium
Hi, I processed some mouse hearts that were in Z-Fix for 24 hrs. then put in 70% for anywhere from 24-48hrs. They were processed on a short run. The epicardium looks good but the mid myocardium is wavy. 15 min 80% 15 min 95 x 2 15 min 100% x 2 15 min Xylenes x2 10 min paraffin x 4 Any suggestions? Thanks. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. MC 1-283 Houston, TX 77030 832-355-6524 832-355-6812 (fax) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
