[Histonet] Fw: Reminder Anatomic Pathology Rounds today Wednesday 9/13

[O'Neil, Beth via Histonet]

From: Smittle, Anne 
Sent: Wednesday, September 13, 2023 7:59 AM
Subject: FW: Reminder Anatomic Pathology Rounds today Wednesday 9/13

Good morning,

MS2 PALM Rounds today beginning around 1:00ish today in the Gross Room, 
continuing on into Histology Labs and finishing up in the Residents Library 
looking at current cases with Dr. Williams. Thank you for your assistance with 
the med school students ---next week two Rounds on Tuesday and Wednesday,

Anne Smittle
PALM Course Coordinator
West Virginia University
2190 HSCN
Morgantown, WV 26505
(304) 293-3779
asmit...@hsc.wvu.edu




-Original Message-
From: asmit...@hsc.wvu.edu 
Sent: Wednesday, September 13, 2023 7:57 AM
To: Smittle, Anne 
Subject: Reminder Anatomic Pathology Rounds today 9/13

Reminder AP Rounds today. Check instructions on the Sign Up List for AP Rounds.



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[Histonet] Leica H 600

[O'Neil, Beth via Histonet]

I would appreciate any feedback from anyone with experience using the Leica H 
600. I recently attended a demo of the unit and we are still hung up on the 
large slide tray it uses instead of the traditional slide racks.   I also spoke 
with another user who indicated that if you need to re-coverslip a slide it is 
very difficult to remove the existing coverslip and when you do the hematoxylin 
is removed from the stain ???  We already know we will need two units to handle 
our workload of approximately 900 H  Any information would be greatly 
appreciated.

Beth O'Neil
beth.one...@wvumedicine.org
West Virginia University, JW Ruby Memorial Hospital
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[Histonet] Histo Chill tissue freezing bath

[O'Neil, Beth via Histonet]

We were given a brand new, neve used, FTS SP Scientific Histo Chill tissue 
freezing bath.  This will be great for our department since we won't have to 
deal with liquid nitrogen.  Our problem is we have no idea how to use it.  The 
company doesn't provide installation or training just an operator manual.  I am 
unable to find any videos on the internet so I'm reaching out to anyone who has 
used this piece of equipment and if anyone knows whether it is not even worth 
trying to use.  We would love to use it for freezing our muscle biopsies for 
neuropath workups.
Thank you
Beth O'Neil,  MT(ASCP)SC, HTLcm, QIHCcm
Histology supervisor/technical specialist
beth.one...@wvumedicine.org


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[Histonet] Frozen tissue from Immunofluorescence

[O'Neil, Beth A. via Histonet]

Would anyone be willing to share with me what you do with residual frozen 
tissue from immunofluorescence testing or frozen tissue remaining from muscle 
biopsy?  CAP requirements only address what you do with residual frozen tissue 
remaining from intraoperative consultations.  We keep the frozen tissue from IF 
in our -70 C freezer and then periodically dispose of the oldest cases in order 
to make room for the newer.  Does anyone out there actually take the frozen 
tissue, after a certain period of time, thaw it out and then process into a 
FFPE block for storage?

Beth ONeil
beth.one...@wvumedicine.org

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[Histonet] Problems with DIF staining of C4d

[O'Neil, Beth A. via Histonet]

We perform manual DIF staining at our facility and C4d has always been a 
problem.  We end up repeating the stain more times than not due to lack of 
staining.  We aliquot the FITC and freeze at -70C until ready for use.  We also 
started doing the same with the C4d.  Sometimes it works and sometimes it 
doesn't.  My pathologist said it is not the FITC but the C4d itself.  I have 
contacted the manufacturer of my C4d but they are very slow to respond.  Any 
suggestions or experiences would be appreciated.

Beth ONeil
WVU Medicine, JW Ruby Memorial Hospital

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[Histonet] Fw: Enrichment Week Starts TODAY

[O'Neil, Beth A. via Histonet]

Histology will get their Med Tech student tomorrow, Feb 23 at 07:00 until 11:00.


From: Schmidt, Ashley A. 
Sent: Monday, February 22, 2021 6:57 AM

Subject: Enrichment Week Starts TODAY


Reminder, we have a student on Enrichment week starting today



AM (7am-11)

PM (11-3:30)

Monday

Managers(7am), Quality Assurance(8am), Path Table(10-11)

Cytogenetics

Tuesday

Histology

Point of Care

Wednesday

Specimen Processing

Bone Marrow/Stem Cell

Thursday

Cytology (8am start)

Morgue

Friday

OFF



Note:  The PM rotation will be in charge of sending the student to lunch with 
the exception on Thursday. The student will report to the Morgue at 12:30pm 
after lunch.





Ashley Schmidt

Manager, Clinical Labs

One Medical Center Drive

Morgantown, WV 26506-8009

Phone 304-598-4241 ext 3223

Fax 304-598-4166



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[Histonet] HSV-2 antibody - Cell Marque was not the problem

[O'Neil, Beth via Histonet]

Last week I posted an inquiry about having problems with Cell Marque's HSV-2 
polyclonal antibody.  I have to retract my statement that it was Cell Marque 
using a different antibody source.   After spending two weeks troubleshooting 
my stainer, troubleshooting the positive control slides, yelling at my Cell 
Marque rep, etc.  I found out that it was the positive control slide and not 
the antibody.  When I originally suspected the positive QC slides (from Cancer 
Diagnostics), I requested a different lot number.  This new lot also failed to 
show positive staining.  Long story short, another call to Cancer Diagnostics 
finally resulted in receiving confirmation that they are having problems with 
their HSV-2 control slides.  It was my misfortune to have opened a new box of 
QC slides at the same time as opening a new vial of HSV-2 antibody which 
resulted in two weeks of headaches.  I will say that Cell Marque/Millipore was 
very supportive through this.  So, for those of you who are using Cancer 
Diagnostics HSV-2 control slides, they are working on a resolution and will 
replace their "bad" slides.  I was told that the staining is "hit or miss, "  
hopefully you all have the "hit" slides.

Beth Oneil, WVU Medicine




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[Histonet] Cell Marque HSV II

[O'Neil, Beth via Histonet]

For those of you who use Cell Marques HSV II polyclonal rabbit antibody 
(382A-18-ASR) were you aware that they sent out information last year stating 
that their antibody source  was discontinued and that they would be using a 
different antibody source (I guess the rabbit died - sorry, I just had to say 
that)?  I wasn't aware of this until I spent many, many days troubleshooting as 
to why my positive control wasn't working.  After contacting Cell Marque 
Technical support I discovered that my product was replaced with 382A-78-ASR 
(they didn't give me any indication that they were sending a different 
catalogue number).  Anyways, now I have to re-optimize and re-validate and I'm 
experiencing difficulty.  Since it is an ASR I am unable to receive any help in 
doing this so I'm reaching out to my fellow histonetters.  Have any of you 
experienced a drastic change in your protocols when using the new polyclonal  
antibody?

Beth Ann O'Neil
one...@wvumedicine.org




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Re: [Histonet] Histonet Digest, Vol 169, Issue 16

[O'neil, Beth via Histonet]

We have a HistosMate from Milestone Medical.  It is wonderful.

 Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC
 Histology Supervisor, Technical Specialist
 Lab:  304 - 293 - 6014
 Office:  304 - 293 - 7629
 one...@wvumedicine.org


--

Message: 2
Date: Fri, 22 Dec 2017 09:19:03 -0800
From: P Sicurello 
To: HistoNet 
Subject: [Histonet] Embedding Mold Cleaning Device
Message-ID:

Content-Type: text/plain; charset="UTF-8"

Good Morning Listers,

There used to be on the market a small gizmo that cleaned embedding molds.
Does anyone have one and can tell me where or who to buy it from?

I know we can use the processors to clean them, but for our circumstances it 
would be more convenient to have one of those table-top mold cleaning machines.

Thank you and welcome to winter.  (where here in San Diego the temperatures are 
in the upper 60's) -  oh wait!  that's because all the fires are heating us up  
;-)

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872 <#>




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[Histonet] Anti-nitrotyrosine

[O'neil, Beth via Histonet]

Would anyone have automated IHC staining protocols for Anti-nitrotyrosine?  The 
antibody is from Santa Cruz Biotechnology #55256.  Any help would be 
appreciated.  Thanks

Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC
one...@wvuhealthcare.com
Histology Supervisor, Technical Specialist
Lab:  304 - 293 - 6014
Office:  304 - 293 - 7629



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[Histonet] Ventana Ultra CD10

[O'neil, Beth]

We are in the last stages of contract negotiations for purchasing two Ventana 
Benchmark Ultras.  During this time period we have been optimizing our current 
inventory of approximately 110 antibodies and validating before our current 
instrumentation is removed within the next two weeks.  My Ventana application 
specialist is unable to successfully optimize CD10 (Ventana clone SP67) on the 
Ventana Benchmark Ultra.  He had me send slides to their 
applications/troubleshooting lab but they told us they won't start working on 
it until next week and then it would take about a week for them to try and 
optimize it.  We are in an urgent rush to get this antibody optimized and 
validated within the next two weeks since it is heavily requested by our 
Hemepaths.  Would anyone be willing to share their Ultra protocols with me?  
Has anyone had similar experiences with Ventana being unsuccessful and having 
to send their slides to their applications lab for work up?  Thank you for your 
help.

Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC
one...@wvuhealthcare.commailto:one...@wvuhealthcare.com
Histology Supervisor, Technical Specialist
Lab:  304 - 293 - 6014
Office:  304 - 293 - 7629



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[Histonet] Ventana protocols for ISH control probes

[O'neil, Beth]

We are optimizing/validating a new Ventana Benchmark Ultra but I need protocols 
for the positive RNA probe (U6  DNA Probe) and the Negative control probe.  Any 
help would be much appreciated.

Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC
one...@wvuhealthcare.commailto:one...@wvuhealthcare.com
Histology Supervisor, Technical Specialist
Lab:  304 - 293 - 6014
Office:  304 - 293 - 7629



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[Histonet] Toluidine blue background

[O'neil, Beth]

We would like to use Toluidine Blue for staining frozen sections of kidney 
biopsy.  We finally are able to get the stain dark enough, but we can't get rid 
of the background caused by the OCT.We've washed with water, alcohols, and 
PBS  with no success.  Is anyone out there using Toluidine Blue for frozen 
staining?  If so, what is your recipe and how do you get rid of the background?
Thanks

Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC
one...@wvuhealthcare.commailto:one...@wvuhealthcare.com
Histology Supervisor, Technical Specialist
Lab:  304 - 293 - 6014
Office:  304 - 293 - 7629



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[Histonet] RE: Mounting media for immunofluorescence

[O'neil, Beth]

We use Vectashield Mounting Medium, catalog# H-1000, Vector Laboratories, 10 
ml.  My pathologists absolutely love it.  It really works for retarding 
photobleaching and really preserves fluorescence.

Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC
one...@wvuhealthcare.commailto:one...@wvuhealthcare.com
Histology Supervisor, Technical Specialist
Lab:  304 - 293 - 6014
Office:  304 - 293 - 7629




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[Histonet] Slide Mate Print Quality

[O'neil, Beth]

In answer to the question about the Slide Mate printing coming off of the 
slides, we had the same issue about a year ago.  At first Thermo though it was 
the ribbon but it turned out the problem was with the slides.  We use the 
Shandon Superfrost Plus (Cat# 6776214).  We contacted Thermo/Shandon and they 
sent us a different lot.  We had to go through about three different lots 
before the problem was resolved.

Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC
one...@wvuhealthcare.commailto:one...@wvuhealthcare.com
Histology Supervisor, Technical Specialist
Lab:  304 - 293 - 6014
Office:  304 - 293 - 7629




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[Histonet] RE: ANP.22970

[O'neil, Beth]

Per CAP checklist ANP.22970, it lists the expected published benchmarks for 
ER/PR, does anyone know where to find them for Her2 or MMR?

Thanks and Happy Friday!!

Tanya



Tanya G. Abbott RT (CSMLS)

Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 
19603-0316 ph  610-378-2635 fax 610-898-5871

email: tanyaabb...@catholichealth.netmailto:tanyaabb...@catholichealth.net

In response to Tanya's question, when I called CAP, they told me that if you 
were to view the Master Checklist, not your customized, it will list several 
references which I have copied for you below:

REFERENCES
1) Wolff AC, Hammond ME. Schwartz IN. et al. American Society of Clinical
Oncology/College of American Pathologists guideline
recommendations for human epidermal growth factor receptor 2 testing in breast
cancer. Arch Pathol Lab Med 2007;131: 18-43
2) Hammond ME. Hayes OF. Oowsett M. et al. American Society of Clinical
Oncology/College of American Pathologists guideline
recommendations for immunohistochemical testing of estrogen and progesterone
receptors in breast cancer. Arch Pathol Lab Med
2010;134(6): 907-922
3) Fitzgibbons PL. Murphy OA. Hammond ME. et al. Recommendations for validating
estrogen and progesterone receptor
immunohistochemistry assays. Arch Pathol Lab Med 201 0; 134:930-935
4) Ounnwald LK. Rossing MA. Li CI. Hormone receptor status. tumor 
characteristics.
and prognosis: a prospective cohort of breast cancer
patients. Breast Cancer Research 2007;9:R6


Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC
Histology Supervisor/Technical Specialist
West Virginia University Hospitals
one...@wvuhealthcare.com
304-293-7629 (office)
304-293-6014 (lab)




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[Histonet] CAP Annual Results Comparison for FISH/ISH

[O'neil, Beth]

Would fellow Histonetters be able to explain how they answer the following CAP 
question:
ANP.22970 For immunohistochemical and FISH/ISH tests that provide independent 
predictive information, the laboratory at least annually compares its patient 
results with published benchmarks, and evaluates interobserver variability 
among the pathologists in the laboratory.
Where would one even find published benchmarks?  Thank you

Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC
Histology Supervisor/Technical Specialist
West Virginia University Hospitals
one...@wvuhealthcare.com
304-293-7629 (office)
304-293-6014 (lab)




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