[Histonet] CA 15-3 and CA 27.29
I was wondering if anyone out there knows of a lab that is performing CA 15-3 and/or CA 27.29 by IHC on FFPE tissue? Thanks in advance. Lisa Roy, HT(ASCP)cm Histology Supervisor Worcester, MA -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyoffi...@labcorp.com or call (877) 23-HIPAA / (877) 234-4722. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Pancytokeratin (AE1/AE3) Protocol
All of a sudden our AE1 is no longer good for the pathologists. Anyone willing to share their protocol for Ventana Benchmark XT or Ultra? I have "tweaked" this protocol so many times. It amazes me how often stains are great during optimization and validation, then months later, everyone hates it!!! UGH. Thanks Lisa Roy, HT(ASCP)cm -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyoffi...@labcorp.com or call (877) 23-HIPAA / (877) 234-4722. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] OJT Histotechs/Training
I currently have 3 open tech positions and don't have any qualified applicants applying for the job. I have recently taken a lab aide that showed interest and aptitude and began OJT. With less than 30 schools in the country actually teaching histology, this is one day going to be the way. Already having a bachelors in biology, my aide qualifies to sit for the ASCP exam once he has completed one full year of tech work and has a pathologist willing to review his work and sign off on the ASCP paperwork. Without going through a traditional program, one must have an associates or bachelor's degree with a certain amount of Chemistry and Science credits. As far as the training, I started with embedding and moved on from there to cutting and then special staining. All along way, working on troubleshooting and documenting EVERYTHING. Some places will hire someone with only a high school diploma as long as they have previous HT experience. I think the specifics of what each institutio n would deem a qualified trainee will vary from place to place. Smaller hospitals or labs may be okay training someone with aptitude that doesn't necessarily fit the ASCP exam qualifications, but large corporations might really insist that the trainee be certifiable at some point. Frankly, I think taking someone that shows an interest and has the knowledge to be a great tech is better than hiring someone that you may not know what you are getting. Doing OJT ensures that you are teaching the candidate exactly how you want things done and not having to accept the bad habits of someone that has been doing it a long time and set in their own ways. Good luck Lisa -Original Message- From: Dessoye, Michael [mailto:mjdess...@commonwealthhealth.net] Sent: Thursday, May 14, 2015 7:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] OJT Histotechs/Training Hello Histonet, I'm curious how people are dealing with on-the-job-trained histotechs. Many people are seeing a shortage in techs, and in my opinion OJT will become more common than it already is. Does anyone have an 'official' training program? Requirements to pass the exam? Qualifications to be able to be trained on-the-job? I'd like to consider having some kind of plan in place when I don't have an HT/HTL applicant but have folks who, if they get the experience, are otherwise qualified to sit for the exam. If anyone has a similar situation or experience to share I would appreciate it! Thanks, Mike Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.netmailto:mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1486 -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyoffi...@labcorp.com or call (877) 23-HIPAA / (877) 234-4722. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HE Stainer Question
Paula Here are my two cents I currently use a Leica Autostainer XL with attached glass coverslipper. It is consistent in its staining and easy to use. The downfall is if you are a large volume lab or just have large volume days, each staining rack holds 30 slides and only one rack can be stained in each batch. The stainer also only has one on board oven, so the throughput of this machine is fairly low. It is only staining 30 slides at a time, with one holding station for the next set. It will run multiple batches concurrently, but gets to a point where it is all backed up. We sometimes have 2 racks staining, one in the oven, one in the loading dock, and some sitting on top of the stainer until it can go on. On the flip side, the Sakura Prisma is a workhorse. It is very similar to the Leica in the sense that it is linear and very consistent in staining. It has two on board ovens and each basket can hold 20 slides. The difference is that the Sakura can stain 3 racks (60 slides) per batch, with two batches in the oven at the same time. That gives you 120 slide throughput for each batch. This stainer also has an attached coverslipper (Sakura Film), but it is film coverslips. I know, I know.no one likes the film coverslips. One advantage to the film, is that the slides are dry almost immediately and can be filed away the same day. No waiting for 3-4 days for the glass ones to fully cure. I can say that the last lab I worked in had the film coverslips and after 10 years, the slides were still in pristine condition. There are many pathologists that do not like to read film covered slides, but once ours got used to it, they had no problems. Some say the film yellows or comes off with the tissue still attached. I can say that I never seen this in my past position. It is very dependent on Xylene only during coverslipping. You cannot use a xylene substitute in the coverslip portion and expect to get good results. Hope it helpsgood luck. Lisa -Original Message- From: Paula Sicurello [mailto:pat...@gmail.com] Sent: Monday, May 11, 2015 5:04 PM To: HistoNet Subject: [Histonet] HE Stainer Question Me again... UCSD is in the market for a new HE stainer for our new hospital opening next year. We need a workhorse, not a prima dona, something with a coverslipper built in would be nice. What do you use? Suggestions gratefully accepted-even from you two Keith and Matt ;) Opinions about the good, the bad, and the ugly (as long as it works really well) will be helpful. Thanks oodles! Paula :-) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyoffi...@labcorp.com or call (877) 23-HIPAA / (877) 234-4722. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HE Stainer Question
Depends on which model you have...hence XL. Ours only has one oven so you're really only getting 3-4 racks stained at a time. -Original Message- From: Simmons, Christopher [mailto:sim...@upmc.edu] Sent: Tuesday, May 12, 2015 8:33 AM To: Roy, Lisa; Paula Sicurello; HistoNet Subject: RE: [Histonet] HE Stainer Question To be fair, a batch of slides for Leica is actually 270- slides, It can run 9 racks at a time, but, the 9th rack from start to coverslip is 3 hours+ You can always skip the on-board oven and place your slides in a slide dryer (most labs have them) and then every 3 minutes you can load a rack (1st xylene step 3 minutes) Then it goes much faster. As for tape..ugh..it is only guaranteed to last 7 years, after that they pull off the slide and take the tissue with it. CAP is starting to frown on this as you need to keep the initial HE slides for up to 10+ years. Chris Simmons B.S., A.S., HTL(ASCP) Supervisor, UPP Dermatopathology 412.864.3880 office 412.612.0881 cell -Original Message- From: Roy, Lisa [mailto:ro...@labcorp.com] Sent: Tuesday, May 12, 2015 8:19 AM To: Paula Sicurello; HistoNet Subject: Re: [Histonet] HE Stainer Question Paula Here are my two cents I currently use a Leica Autostainer XL with attached glass coverslipper. It is consistent in its staining and easy to use. The downfall is if you are a large volume lab or just have large volume days, each staining rack holds 30 slides and only one rack can be stained in each batch. The stainer also only has one on board oven, so the throughput of this machine is fairly low. It is only staining 30 slides at a time, with one holding station for the next set. It will run multiple batches concurrently, but gets to a point where it is all backed up. We sometimes have 2 racks staining, one in the oven, one in the loading dock, and some sitting on top of the stainer until it can go on. On the flip side, the Sakura Prisma is a workhorse. It is very similar to the Leica in the sense that it is linear and very consistent in staining. It has two on board ovens and each basket can hold 20 slides. The difference is that the Sakura can stain 3 racks (60 slides) per batch, with two batches in the oven at the same time. That gives you 120 slide throughput for each batch. This stainer also has an attached coverslipper (Sakura Film), but it is film coverslips. I know, I know.no one likes the film coverslips. One advantage to the film, is that the slides are dry almost immediately and can be filed away the same day. No waiting for 3-4 days for the glass ones to fully cure. I can say that the last lab I worked in had the film coverslips and after 10 years, the slides were still in pristine condition. There are many pathologists that do not like to read film covered slides, but once ours got used to it, they had no problems. Some say the film yellows or comes off with the tissue still attached. I can say that I never seen this in my past position. It is very dependent on Xylene only during coverslipping. You cannot use a xylene substitute in the coverslip portion and expect to get good results. Hope it helpsgood luck. Lisa -Original Message- From: Paula Sicurello [mailto:pat...@gmail.com] Sent: Monday, May 11, 2015 5:04 PM To: HistoNet Subject: [Histonet] HE Stainer Question Me again... UCSD is in the market for a new HE stainer for our new hospital opening next year. We need a workhorse, not a prima dona, something with a coverslipper built in would be nice. What do you use? Suggestions gratefully accepted-even from you two Keith and Matt ;) Opinions about the good, the bad, and the ugly (as long as it works really well) will be helpful. Thanks oodles! Paula :-) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyoffi...@labcorp.com or call (877) 23-HIPAA / (877) 234-4722. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyoffi...@labcorp.com or call (877) 23-HIPAA / (877) 234-4722
RE: [Histonet] (no subject)
How long do you bake slides for before staining, at what temperature? Does your stainer use agitation? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carolyn Nelson Sent: Thursday, April 30, 2015 11:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi, I was hoping someone can help me with tissue falling off the slides. I have tried regular slides with and without adhesive in the water bath. Charged slides with and without adhesive in the water bath. I have not changed the type of slides I’m using. All the chemicals are fresh in the processor and the stain line, as well as the paraffin in the processor. It is the worst on needle bx ( prostate and breast ). I am SO frustrated, any help would be greatly appreciated! Carolyn Sent from Windows Mail -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyoffi...@labcorp.com or call (877) 23-HIPAA / (877) 234-4722. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Over- decalcified tissue
Good article on IHC world about restoring nuclear detail to over decalcified tissueit is called Problem Number 23. Good luck. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Wednesday, April 29, 2015 12:21 PM To: pablo.sanc...@usc.es Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Over- decalcified tissue I would suggest not allowing decalcification to be extended. Better to leave in fixative until you can control the time of endpoint of decalcification. What type of decal solution are you using? I am not a big fan of trying to adjust the chemistry of the stain to compensate for over decalcification. Is there opportunity to submit another sample? Sent from my iPhone On Apr 29, 2015, at 9:08 AM, pablo.sanc...@usc.es wrote: As I usually process gross pieces of bone -that need abouth thirty hours decalcifying- I suffer the same nuissance. Also would thank any hint. Pablo Sanchez Laurie Colbert lcolb...@pathmdlabs.com escribiu: I have tissue that was left in decal over the weekend and now has very poor nuclear staining. Is there a fix for this so that I can get better nuclear staining (other than restaining for a long time in the hematoxylin)? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyoffi...@labcorp.com or call (877) 23-HIPAA / (877) 234-4722. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Nuclear Artifact
Hi HistoNetters: I have run into quite a problem. My lab currently processes all tissue types from 3 different sites. Recently, we have been getting complaints from one of the sites that the biopsies have a nuclear artifact. It is described as washed out or poor to no nuclear detail. Pictures have been uploaded (Nuclear Artifact). The Medical Director at said site is convinced that a processor error is occurring. Our site is not seeing this on any of our slides. Biopsies from all three sites are processed and embed together. We have done all trouble shooting that we can think of. Leica service has come to inspect our Peloris processor and all areas checked out as functioning properly. The problem is not consistent daily. Seems to be worst toward the end of the week. We have been running the same processing protocol, staining protocol and cutting protocols for years now. This problem has just developed over the last 2 months. Any ideas, no matter how far-fetched, would be greatly appreciated at this point. Lisa Roy, HT(ASCP) Histology Supervisor LabCorp at St. Vincent Hospital 123 Summer St Worcester, MA (508)363-9420 -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyoffi...@labcorp.com or call (877) 23-HIPAA / (877) 234-4722. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
