[Histonet] Exciting position at the University of Wisconsin~Madison
Hello all, The School of Veterinary Medicine at the University of Wisconsin, Madison has a full-time position for a Necropsy Technician. The position is responsible for assisting the veterinary pathologists in the biopsy and autopsy service - much like a human hospital but with a much wider variety of species from the veterinary teaching hospital, (dogs, cats, horses, cows), the DNR, (wolves, deer, raccoons, birds), and the Milwaukee County Zoo, (fish, primates, kudu, rhinos, penguins, and many other exotic species. I'm not gonna lie; It's pretty cool. This is a terrific group of co-workers and pathologists who are dedicated to furthering the knowledge of veterinary medicine. Here's the link... NECROPSY TECHNICIAN at UW-Madison (wisc.edu)<https://jobs.hr.wisc.edu/en-us/job/509806/necropsy-technician> Cheers, Sandy Sandra Cheasty, HT(ASCP), Emerita School of Veterinary Medicine University of Wisconsin ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Two University of Wisconsin Positions
Hello, We have two positions open in anatomic pathology at the School of Veterinary Medicine at the University of Wisconsin, Madison, the links are below... https://jobs.hr.wisc.edu/en-us/job/509270/histology-technology-supervisor (application deadline June 10th) https://jobs.hr.wisc.edu/en-us/job/509189/anatomic-pathology-laboratory-manager (application deadline June 3rd) Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cassette Labels
Hello,r Has anyone has used printed labels successfully on cassettes that can withstand xylene/paraffin processing and long term storage? (I am NOT looking for a cassette printer...) Thanks! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Block Holder for Leica Rm2255
Hello all, The handle snapped off our RM2255 microtome block holder, and the replacement we were sent does not open the clamp far enough. (We are currently managing by using an allen wrench in the drilled out hole...) Does anyone have any suggestions? Cheers, Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] New Tissue Processor?
Hello all, Our lab is looking to replace our 10 year old VIP6. We process anywhere from 50 to 200 cassettes daily. Any recommendations are appreciated. Thank you, Sandra Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Autopsy Question
Hello all, Actually it's a necropsy question for those of you who work in veterinary medicine. We perform necropsies on a very large variety of vertebrates that require removal of the spinal cord. (From hedgehogs to bovines.) What equipment are you using to remove spinal cords on different species? Cheers, Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Deep Water Bath with Lighting
Ho-ho-ho all, I'm looking for a deep water bath, (3" minimum), that has an interior light source. Most of them seem to be only 2" tall, or have a slanted front control panel that none of us in the lab want. Does anyone know of a brand of flotation water baths that are 3" deep and have interior lighting? Cheers! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] 10% NBF Substitutes
Hello, Can anyone comment on the current formalin substitutes Excel PLUS and/or FineFIX? -Cost -Benefits -Drawbacks -Performance in tissue processors Thanks! Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Jeepers Creepers, Coverslipping Peepers!
Hello all, Does anyone have a source for oversized coverglass? (35x50, 35x60, 45x50, 45x60) We section a lot of globes from the ophthalmology lab, some of which are from horses, cows, etc. that are put on the larger slides. Fisher used to carry their own brand, but it's apparently discontinued them, and their "alternate" product is $50/oz! Thanks! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Galileo Microtome
Hello all, Has anyone had experience using the Galileo Series 2 microtome, made by Diapath? Thanks! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Motorized Microtome Opinions
Hello all, Our only motorized Microm microtome is slowly dying, and I'm looking for a replacement that can be used manually, semi, or fully automatic. I'm leaning towards Leica as I have had good luck with their manual microtomes. Please, experienced histotech comments only, no sales pitches... Cheers, Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thyroglobulin TGB04+TGB05 Not Working in Canine and Feline
Hello, We had to switch sources (to Novus) for Thyroglobulin TGB04+TGB05 and cannot get it to work in canines or felines. Data sheet suggests 1:100, pH6 citrate antigen retrieval. We've also tried zero AR and pH9 EDTA AR, to no avail. Any suggestions? Thanks! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica RM2235 Block Clamp Lever snapped!
Hello all, The block clamp lever of one of our Leica RM2235 microtomes snapped off. The broken end is still in the (threaded?) hole. Any advice or source for fixing/replacing this is most appreciated. Thanks, Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Softening Calf Hooves
Hello all, I have 1 inch thick cross sections (band saw) of calf hooves. The pathologist has already decaled the bone, and now we have to soften the hooves so she can take thinner sections for processing. * Which is better, potassium hydroxide or sodium hydroxide, and what concentration? * How often should they be checked for softening? * What is the relative air speed of an un-laden swallow? (European) Thanks for your help! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC Control Slide Longevity
Hello all, Do any of you have to cut any of you antibody controls "fresh"? We currently cut the MUM-1 fresh, and the Synaptophysin. (We seldom do a Syanpt., and have found the fresh cut patient tissue has been positive while the not-so-fresh positive control slide is negative. Any comments are - as always, are appreciated. Cheers, Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Used Lab Vision 720D Immunostainer
Hello all, Does anyone know of a company who buys used Histology equipment? Our Lab Vision 720D Immunostainer still works fine, and had a full PM in July 2018. (We purchased a new IHC stainer that is better suited for research.) Cheers, Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ortho-keratotic Mineralizing Odontogenic Cyst
Hello all, Does anyone have any ideas on de-mineralizing an ortho-keratotic, mineralizing, odontogenic cyst? It is so hard the pathologist cannot cut it into small enough pieces to fit into a cassettes. (It's been in decal for days with no change.) I was thinking of taking one of the chunks and putting it in potassium hydroxide under the hood, and monitoring every half hour or so. Anyone have any other ideas? Cheers, Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sakura Tape Coverslipper Advice Needed!
Hello all, Our Sakura tape coverslipper is doing something I've never encountered before - the lever that holds the blade and goes up and down and cuts the tape, has gotten sticky and slow. It will chop down fine, but has a hard time returning to the up position. Before I take the back off, I'm just wondering if this has happened to anyone else, and if so, how it was rectified. Cheers! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PT Module Spare Part
Hi all, Does anyone know where I can get my hands on some PT module reagent level sensors? (The black bar on the back of each tank.) Our pH 9 AR destroyed it in one tank, and the pH 6 sensor now has some wear and tear also. Otherwise the PT module works great. Thanks! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD204 Anti-MSR1 monoclonal, clone SRA-E5
Hello all, Does anyone know where I can get CD204, Anti-MSR1 monoclonal, clone SRA-E5? Thanks! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ultra Clean Diluent for IHC
Hi all, It seems Fisher has stopped carrying the "Ultra Clean Diluent 125ml". (Item TA-125-UC). Can anyone recommend someone else who carries it, or a likely replacement? We use the Po-Link 2+Broad HRP detection kit. Cheers! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC Stainer
Hello all, We are in the market for a new IHC stainer. (Our Lab Vision 720 is no longer reliable.) We average about 20 slides a day, but sometimes get research projects that are 50+ slides. Not interested in a closed system; it is for a veterinary histology lab, and we need the flexibility to tweak antibodies for dogs, cats, horses, and zoo animals. I'd really appreciate feedback from users who are in a similar lab setting. Cheers! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Hard Water and Auto Stainers
Hello all, Thanks for everyone's response to error 90 on our Prisma stainer; it has been cleared! Does anyone use filtered, softened, or DI water in their H stainer to avoid clogging up the system? Thanks! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Vacuum Breaker Error on Prisma H Stainer
Hello all, We have been getting an occasional, (maybe once every 20 runs), error 90 on our Prisma stainer. (Vacuum Breaker Error) Has anyone encountered this? Does it have to do with the incoming water lines? I'm trying to avoid a costly visit by a Sakura vetted repair tech. Thanks! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Lab Vision 720-2D Parts
Hello all, I am in need of some parts for our Lab Vision 720-2D IHC stainer that don't cost an arm and a leg. (Plunger, plastic piece that holds the wide top of the plunger, tubing, stopcock, connections.) If you e-mail me, I can send a picture of exactly what I'm looking for. Cheers! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Grossing Blade for Ophthalmology Globes
Hello all, Can someone recommend an economical disposable blade for grossing eye globes? Sharp enough to slice through sclera smoothly, strong enough to finish the cut through the lens. Cheers! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Paraformaldehyde Fixed Tissue
Hi all, We are having difficulty sectioning mouse tissue, (brain, spinal cord, liver, and spleen), on paraformaldehyde fixed tissue. Has anyone had issues with paraformaldehyde fixed tissue? They were processed routinely, starting in 10% NBF, with other tissues, and we are cutting them at 3u. Thank you! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cardboard File Drawers for Blocks
Hello everyone, Does anyone know of a company that sells the cardboard drawers and drawer dividers for filing blocks, separate from the outer cardboard file that the drawers slide into? We came into possession of 25 outer files with no drawers, which means we need 200 drawers and dividers. Thanks! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] "Supa Mega Clamp"
Hello all, We use the "Supa Mega Clamp" (Ted Pella) for sectioning large tissues in the oversized cassettes. Lately, it seems not to grip as tight as it did when we received it a month or so ago. The literature is little more than photos of how to attach the clamp to our microtomes. Has anyone experienced this? Is there an adjustment that can be made? Thanks! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Pathology Band Saw
Hello, Can anyone recommend a band saw for pathology? We are looking for a new band saw for use in necropsy and research. It has to be a "meat" band saw, as workshop band saws aren't compatible with wet tissue. A table-top model is preferred, and cheaper than a floor model. (Plus, the floor models all seem to come with a sausage-maker, which is pretty gross.) If you have used a table-top band saw in your pathology lab, I'd appreciate hearing from you. Cheers! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Extra Large Cassette Clamps for Microtomes
Hello all, I am looking for opinions on giant block clamps for the Leica RM2235 and Microm HM355S microtomes. * I know CellPath makes one, are their others? * Is it easy to swap them out for a regular clamp, or is it advisable to dedicate one microtome for these huge cassettes. Thank you! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Refurbished Leica RM2135
Hi everyone, I have a newly refurbished Leica RM2135 at my disposal, but the knife holder is for high profile blades. 2 questions. 1-Is it that different using high-profile blades vs. low. (I have used low-profile blades forever.) 2-There is a slot on the bottom of the blade holder, and I seem to remember that it can be converted to a low-profile holder by sliding an narrow metal strip into that slot. I know I've done this before, but it was long ago, and the used equipment guy says there's no such thing. Thank you! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Long Pipette Sharps Disposal Container
Hello all, Can anyone recommend an economical sharps disposal container for long plastic pipettes? Cheers, Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thyroglobulin Ab-6 (Clone TGB04+TGB05)
Hello all! Does anyone have a source for Thyroglobulin Ab-6 (Clone TGB04+TGB05), 200ug/ml? We use it primarily on canine and feline, (It also works on guinea pigs,). Thanks much! Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microm HM 355S Issues
Hello, We have 2 Microm HM355S motorized microtomes. We use the motor to move the block close to the blade, but turn the wheel manually when sectioning. Both are "jumping" forward on their own occasionally while sectioning, which is unsettling and chunks into the block. One of them also backs up on its own as you're manually turning the handle, just as you're about to get a ribbon. The stepper motor has been replaced on one with no improvement. Any suggestions, remedies, or tales of similar issues and how they were resolved would be most appreciated. Thanks, Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Program for organizing and re-naming digital gross photos
Hello all, Picasa, the program we currently use for re-naming and organizing our gross digital photos, will no longer be supported by Google. If anyone can suggest another program for this purpose, please let me know. We don't need any fancy editing of the photos themselves other than cropping and adjusting brightness; mostly just for re-naming and organizing by species. Thanks! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Animals Need Histologist Too!
Hello All! We are looking for a dedicated Histologist, with at least 1 year of experience in a full service histology lab, to join our Veterinary Pathology team. Our Histology lab serves the Veterinary Medical Teaching Hospital in Madison, Wisconsin, as well as many research needs at the School of Veterinary Medicine. We are a full service lab providing processing, embedding, microtomy, histochemical and immunohistochemical staining. Full-time, Days, Monday through Friday You can reply to me directly, or follow the link below to apply. http://www.ohr.wisc.edu/Weblisting/External/PDSummaryApply.aspx?vacid=97933=35062 Thank you! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Hematoxylin Precipitate
Hello all, Has anyone using Richard Allen Hematoxylin-2 noticed an odd artifact on the slides after using the Hematoxylin for more than a few days on their stainer? We are seeing small spore or pollen-like blue dots here and there on the slides. It is not coming from the water bath or our water supply on the stainer. I used sterile gloves, opened a new case of slides, dipped them in DI water, then in the RA Hematoxylin 2 on the stainer, then in DI again, air-dried and coverslipped them, and the blue dots were there. The only way we got rid of the blue artifact was to use new RA Hematoxylin-2 every 2-3 days, which is a bit expensive. Thanks for your input, and if you can recommend a different, reasonably priced hematoxylin, that would be awesome. Cheers, Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cheap Disposable Blades for Facing In
Hello everyone, Does anyone have a source for cheap, low-profile blades for facing in blocks? Thanks! Sandy Sandra J. Cheasty, HT (ASCP) Histology Necropsy Supervisor, President Keith Richards Fan Club UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Preventative Maintenance Fees Gone Wild
Does anyone else think that $8,266 sounds a bit high for a PM on a Lab Vision Immunostainer? Does that price include a Happy Ending? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Temp Histologist Needed! Madison Wisconsin
Hello all, My lab needs a temp Histologist from approximately June 25 - August 15th. (One of my techs had a bicycle accident and broke his collarbone among other things, and there are several vacations coming up for other staff as well.) I would appreciate suggestion of reputable temp agencies; I haven't needed to use one since I worked in Anchorage, and that was last century! Thanks for your help! Sandy Sandra Cheasty Histology Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC Buffer (wash)
Hello everyone, Got your Christmas shopping done? (Didn't think so...) Anyway, I'm looking for an IHC PBS/Tween buffer wash concentrate that is comparable to the very expensive Lab Vision PBS/Tween buffer wash. I see some at VWR and FISHER (no shipping charges for our lab) that are 0.5% and 1% Tween 20 washing buffer in PBS. Which percentage is best for IHC on a Lab Vision stainer using a polymer detection kit? As usual, your input is appreciated. Sandy P.S. I know I can make up my own from scratch, just not interested in doing that at the moment... Sandra Cheasty Histology Necropsy Supervisor UW-Madison, School of Veterinary Medicine 608 263-1680 (Office) 608 263-1055 (Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Issue with Henry's Wonder Wax Enhanced
Hello fellow Techs, Has anyone noticed a change in the Henry's Wonder Wax, Enhanced? (Used for embedding, not infiltrating.) We have noticed that it is a lot stickier when we are sectioning. (To our picks, forceps, and fingers.) We have checked our temperatures, and all is well. I have asked the company, but they have not replied. Can anyone recommend another embedding paraffin? (We switched from Paraplast Plus about a year ago when we noticed changes after the original manufacturer was bought out.) Thank you, and have a safe food and family filled Thanksgiving! Sandy Sandra Cheasty Histology Necropsy Supervisor UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD204 Clone SRA-E5
Hello everyone, Does anyone know of a U.S. source for the antibody CD204, Clone SRA-E5? (Marker of canine histiocytic sarcoma.) Thanks! Sandy Sandra Cheasty Histology Necropsy Supervisor UW-Madison School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Parts for Sakura Tissue Tek 5 Embedding Center
Hello all you Histologists, Does anyone know of a used Histology equipment company that sells Sakura parts? We have a fairly new embedding center, (but past warranty), that needs a single heater unit. Sakura only sells the entire dispenser unit for nearly $8,000. I don't mean to offend any Sakura fans, but I don't think that's right. Thanks, Sandy Histology Necropsy Supervisor UW-Madison ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] DAB source needed
Hello All, Can anyone recommend a DAB kit that is reliable and reasonable priced? Also, can someone shovel my driveway? Thanks! Sandy Sandra Cheasty Histology Necropsy Supervisor UW-Madison, Wisconsin ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica-Surgipath EM Paraffin Issue
Has anyone else noticed a change in the EM400 embedding medium from Surgipath? We have not changed anything in our embedding, but now the EM400 results in cracks in the blocks. Leica says although the labeling has changed to indicate the EM400 is now also for infiltrating, the paraffin has not changed. Thanks, Sandy SVM ~ UW Madison ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC - PGDFR reactive in Canines
Hello, Does anyone have experience using PGDFR in canines and if so, is there a canine species control that is known to react with PGDFR? Thank you, Sandy Sandra Cheasty Histology Supervisor UW-Madison School of Veterinary Medicine 608 263-1680 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Formalin and Xylene Monitoring Badges
Happy Halloween... I'm looking for a source of monitoring badges for formalin and xylene, both the 8 hour TWA and the STEL. Thank you, Sandy Sandra Cheasty Histology Supervisor UW-Madison School of Veterinary Medicine 608 263-1680 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD20 suddenly has high background
We do CD20 on canine and feline samples and are suddenly getting lots of non-specific background. We use: CD3, F7.2.38 from DAKO diluted 1:400 LSAB+ and DAB+ Avidin and Biotin block I even tried it with a polymer detection kit, but to no avail. Has anyone else had recent difficulty with this T cell marker? Thanks, Sandy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
