[Histonet] See You Soon
Hey guys and gals...I have taken a new position as Pathology Supervisor at North Austin Medical Center here in Austin...so I am unsubscribing for now, but have no fear...I will be back ASAP with my new email address. Hope everyone has a great Turkey Day!! Signing off for now... Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PAXgene Tissue Container
Has anyone ever used this? Apparently it contains two proprietary reagents (a fixative and a transport media). They specifically say it's not formalin (so alcohol or the likes I assume). Just curious if anyone has used it and how it worked for them. Thanks http://www.qiagen.com/products/catalog/sample-technologies/rna-sample-technologies/dna-rna-protein/paxgene-tissue-containers#productdetails Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] In situ PCR
Anyone out there do this? If so, during the PCR step you are amplifying your gene of interest, where does the amplified product go? Each step of the PCR (from how I am understanding this...I'm new to molecular biology protocols...) separates the double stranded sequence then copies it, and this goes on and on for 30-40 cycles...where does the product go? Does it just wash off? If not how is it binding to the tissue? Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] NSH emails
Does anyone know how to get an email address of someone who presented? If not does anyone have the addresses for Melanie von Brandenstein or Heike Goebel? Thanks Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Question about fixation terminology
http://www.ihcworld.com/_protocols/general_ICC/fixation.htm This article specifically addresses the differences. You are right about the cross linking, but alcohol and acetone are still considered fixatives. Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jones, Lynne Sent: Thursday, October 03, 2013 2:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question about fixation terminology Hello - Our research group does a fair amount of autoradiography with frozen sections and we sometimes perform IHC or routine stains. I am not a histologist (nor do we have one in our current group), so I assumed that the correct answer was alcoholic formalin, because the other options were either inappropriate or not fixatives. I was taught (by an old-school cell biologist) that both alcohol and acetone act as dehydrating agents when used on frozen sections, and are not true fixatives, because they don't cross-link proteins. Real fixatives don't just preserve against decay, they also modify the tissue (i.e., cross-linking or denaturing proteins). I am pretty sure I was taught that acetone does not fix tissue, and that fixing tissue to a slide is an imprecise/slang term derived from affix. (This was at least a decade ago, so my memory could be faulty.) How is acetone a fixative? I thought it just replaced water, and preserved the structure of frozen sections by drying them out. (Please be kind - I'm not a histologist. I've sat at the cryostat sectioning mouse brains, and I know when we use it, but I don't understand what the acetone actually does.) How do you describe the difference between preserving tissue by drying (with acetone) and cross-linking the proteins (with alcoholic formalin)? I would really appreciate clarification from the guru's on HistoNet. I no longer spend much time in the lab, but I edit our manuscripts, so try make sure we use the correct terms in describing how the work is done. (Some reviewers get picky about terminology.) Thanks, Lynne Jones Lab Manager Radiochemistry Research Group Radiological Chemistry and Imaging Lab Washington University School of Medicine St. Louis, MO Message: 2 Date: Thu, 3 Oct 2013 10:21:42 -0400 From: Lee Peggy Wenk lpw...@sbcglobal.netmailto:lpw...@sbcglobal.net Subject: Re: [Histonet] HT HistoDeck question... To: Watson, Linda linda.wat...@bms.commailto:linda.wat...@bms.com, Stephenson, Sheryl sstephen...@lifecell.commailto:sstephen...@lifecell.com, histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu Message-ID: 335B9C2DCE414D1CB831DCCB07DEDC94@HP2010 Content-Type: text/plain; format=flowed; charset=iso-8859-1; reply-type=original I agree, there is probably more than one correct answer to this question, depending upon whether you are planning on doing stains for lipids, IHC, immunofluoresence or muscle enzymes. But I don't think (A) full strength 37-40% formaldehyde solution would ever be the correct answer. Unless you put a gauze in the bottom on the coplin jar, pour a little conc. formaldehyde on the gauze, put a lid on the coplin jar, and fix the section in formaldehyde vapors. But the question does say SOLUTION, not VAPOR. So I still think A is wrong. And most likely full strength acetic acid (C) is wrong - would eat the tissue off the slide. That leaves cold acetone (B) , which is good for some antibodies and some enzymes, or alcoholic formalin (D) which might be OK, but most of the time things either like alcohol and hate formalin, or they like formalin and don't like alcohol. So I would think most FS that we want to fix would not particularly like alcoholic formalin. And none of the solutions listed are good for lipids. So, given the question (with incomplete information) and the choices of answers, I would still side with (B) cold acetone. Now - a little aside - for the questions on the ASCP HT and HTL exams - if it is a new question, the people on the HT/HTL exam committee would be looking at it intensely before it goes on the exam for the first time. If the committee people are having problems answering it (like we are here), the question would be reworked until all the issues are resolved (such as putting in for lymph node IHC into the question). If it makes it past the committee, and the stats from the exam show that many people are having problems answering it, the question is pulled from the exam and is not used in the person's score. The question is then sent back to the HT/HTL exam committee, to try to figure out why examinees were having problems, and the question reworked again. As someone who has written exam questions at
RE: [Histonet] Unregistered HT
I don't think that histotechs are disrespected at all. There is always going to be that one person in any job that thinks they are better than you because of whatever reason. HTs are not the highest paid people in the lab (or the lowest for that matter), and I think a lot of times people in management, PhD, or MD positions see us in a light that mirrors our salaries. The fact of the matter is these days you have to basically have a college degree to become a HT, and hopefully as the old-timers who didn't have to have a degree retire out of the field we will be paid more according to the education that a lot of us paid so much money to obtain. I still hold firm to the belief that you get what you give. If you act like someone who should be disrespected you will be. If you suggest new ideas for improvement, go over and beyond your pay grade, then eventually you will be paid for that financially and with respect. If I couldn't live like that I think that would be a very difficult and frustrating way to live every day. Therefore, I choose to have a good attitude about being away from my family everyday (because I have to have a job), and do the best I can to make others have a reason to respect me. Just my two cents...I will step down from my soapbox now =) Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Wednesday, September 11, 2013 1:15 PM To: Rene J Buesa; Clare Thornton; 'Emily Sours' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Wow René, I didn't realize you had such a demeaning and disrespected career. I have been in this profession for almost 40 years. I have worked for some of the greatest pathologists you could ask for. They have respected me, asked my opinions and shared a great deal of time at the microscope with me. Maybe it has to do with attitude.. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, September 11, 2013 1:30 PM To: Clare Thornton; 'Emily Sours' Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Unregistered HT The issue remains the WE cannot impose on others how we are viewed. No matter how many brain storming meetings there are about demanding respect the issue remains that those who have to respect us have to do so. Short of a national strike (absolutely impossible to achieve) or a national union (that will be fought to the death) the only thing we can do is to stand for our rights of being treated as professionals every time somebody, either pathologist or administrator, disrespects us. And who, may I ask, will be able to do that in an economic situation as the present one? Who is going to risk his or her job just to demand respect? That is an illusion. Unfortunately the economic situation impedes any action and those who can will keep disrespecting us as long as they can get away with it. Having a good position statement about what does it mean to be a professional is an exercise in futility that probably for those who wrote it may have meant being able to have 1 or 2 days paid without actual work to do. René J. From: Clare Thornton cthorn...@dahlchase.com To: Clare Thornton cthorn...@dahlchase.com; 'Emily Sours' talulahg...@gmail.com Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Wednesday, September 11, 2013 1:20 PM Subject: RE: [Histonet] Unregistered HT My apologies, the editorial was in the September 2011 issue of Journal of Histotechnology. I have a pdf of it, will be happy to email. Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthorn...@dahlchase.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Clare Thornton Sent: Wednesday, September 11, 2013 10:20 AM To: 'Emily Sours' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Look for a position paper presented by members of the NSH BOD in the Journal of Histotechnology after the national convention in Vancouver last year. I'm not sure of the exact issue (thought I still had it on my desk). This will explain what being a laboratory professional is and why it is so important that as histotechnologists we are viewed as such. Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthorn...@dahlchase.com -Original Message- From:
RE: [Histonet] Unregistered HT
So as not to have my inbox completely filled with emails... None of the below statements was meant in any way as disrespectful to ANYONE! 1. Old timers. Just a term I have heard used by people who have been in the field for a long time. I've been doing histology since 1999...I do not consider myself one of these yet. 2. A degree. I am not saying that all old timers are without a degree or any post HS education. Correct, many non-degreed workers are better than a degreed one. I just think someone with a college degree should be paid accordingly. If they are horrible at their job...they will probably be fired. If you didn't finish your degree and still want to...go do it. 3. Chill out people...it's Wednesday...sheesh... Some of the emails I have got offline completely demonstrate my point...give respect and you will be respected. If you send me a tacky email...I'm probably going to be tacky right back to you... 4. These were all just my opinions...take it or leave it. I hope everyone has a great rest of your day, and some people I think need to go home and have a drink =) Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: Marcum, Pamela A [mailto:pamar...@uams.edu] Sent: Wednesday, September 11, 2013 1:57 PM To: Sarah Dysart; Blazek, Linda; Rene J Buesa; Clare Thornton; 'Emily Sours' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Sorry Sarah some of us old timers do have BS and even MS degrees and still have to fight for every dime. The whole field needs to be improved without any thought of how long someone has been in Histology. We have the issue of people who have HTs being called HTLs due to the way the two registries were created. In the early 1980s the only thing you could get was the HT registry no matter how much education you had worked to get. Then we got the HTL for management skills as it was stated and for several years HTs could grandfather in with no additional education above HS. Then in about 1984 the HTL started to require the education level of BS. Unfortunately, since the two registries were to some extend mixed by the overlap of needing or not needing a degree the whole field is confused about who is what. I have been called a histologist before I took my HT (with a BS) and HTL although I did not bother to grandfather in. Yet I am called both and really don't care which I am called as long as I am respected. An MS made no difference either way. The real issue is most administration people and HR Departments don't know the difference between an HT and HTL and have no idea what to do. I have seen HTs with a HS call themselves HTLs and no one knew the difference at the administration/HR levels. (Some of those older people who started and may not even have a HS degree yet; are among the best histologist I have seen in 50 years. Some with BS and/or MS degrees were terrible. Some MTs came over with CP pay and could be great or awful.) Education is not answer. The issue is straightening out who we are and what we do as either registry area. Here we had a salary survey and found most of the employers just paid the same for everything to avoid the issue or due to total misunderstanding of their even being a difference. Now everyone is the same. No one knew how to solve it to everyone's satisfaction and the histologist were not asked. If you can say you have a BS you may get paid a little higher and only have an HT and be called an HTL. Pam Marcum -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart Sent: Wednesday, September 11, 2013 1:30 PM To: Blazek, Linda; Rene J Buesa; Clare Thornton; 'Emily Sours' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT I don't think that histotechs are disrespected at all. There is always going to be that one person in any job that thinks they are better than you because of whatever reason. HTs are not the highest paid people in the lab (or the lowest for that matter), and I think a lot of times people in management, PhD, or MD positions see us in a light that mirrors our salaries. The fact of the matter is these days you have to basically have a college degree to become a HT, and hopefully as the old-timers who didn't have to have a degree retire out of the field we will be paid more according to the education that a lot of us paid so much money to obtain. I still hold firm to the belief that you get what you give. If you act like someone who should be disrespected you will be. If you suggest new ideas for improvement, go over and beyond your pay grade, then eventually you will be paid for that financially and with respect. If I couldn't live like that I think
[Histonet] Flourescence
So...just got presented with a task...while my confocal experience is very limited I'm willing to learn new thingsthe PI wants to fluorescently (sp?) tag our drug to see where it ends up in the cell. Will a tag survive processing or does it need to be labeled somehow after it's processed? I can use all the help I can get! Thanks histo-peeps! Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC controls
So, I am working on some IHC stuff. I need control for mouse tissues. While I have just about every normal tissue you could ask for, several of these antibodies say the positive control is lung cancer, or breast cancer, or prostate cancer. While I have all of these in human I don't have them in the species I am staining in...mouse. Also, a couple of them say tonsil...do mice even have tonsils?? Anyhoo, what is ya'lls suggestion on this one? Do I stain the appropriate tissue just in the wrong species (human), or does someone know where I can purchase mouse control slides and/or blocks? Thanks Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Plastic/Plexiglass Incubation chamber for IHC slides
It's not the old days in research world...I still do all my IHC by hand =) I use a vegetable steamer I bought at Walmart and Coplin jars full of the retrieval solution...just an inexpensive solution if you need one? Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Brent Heller Sent: Monday, July 08, 2013 1:58 PM To: 'Bea DeBrosse-Serra'; 'Walter Benton'; davidclar...@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Plastic/Plexiglass Incubation chamber for IHC slides Start heating. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra Sent: Monday, July 08, 2013 11:57 AM To: 'Walter Benton'; davidclar...@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Plastic/Plexiglass Incubation chamber for IHC slides We do IHC by hand most of the times.. http://www.newcomersupply.com/products/slide-staining-trays-ihc Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Walter Benton Sent: Monday, July 08, 2013 11:20 AM To: davidclar...@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Plastic/Plexiglass Incubation chamber for IHC slides http://www.ihcworld.com/products/IHC-Staining-System.htm I have not used, but it seems that it would work like the old ones. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of davidclar...@comcast.net [davidclar...@comcast.net] Sent: Monday, July 08, 2013 2:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Plastic/Plexiglass Incubation chamber for IHC slides Hi Histonetters, I hope you all had a wonderful Independence Day! Do you guys remember in the olden days before automation when we did IHC by hand? I'm looking for an incubation chamber that allows us to incubate our slides overnight. The one I have right now is about 20x6x6 with a lid and allows for about 4 flat racks of slides to be placed inside. Any information would be appreciated either if you have one or know of a company that can make one. Cheers! David Clark Oregon Health Science University Portland, Oregon ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: FFPE PCR
I do the PK digestion at 55C overnight and get the best results Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of M. Kap Sent: Sunday, June 23, 2013 12:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FFPE PCR ProtK digestion at 55 degrees C for half an hour will do fine There is also a new FISH like technique to detect single RNA molecules in situ, can't remember the name atm. But it works with so called Z-probes and it's very specific and sensitive. Especially when you want to look at virus in tissue it would be quite intersting to see where the virus is situated. You may even consider PAXgene fixation from now on, great histo/molecular combo fixation. Best regards, Marcel Kap Verstuurd vanaf mijn iPad Op 23 jun. 2013 om 19:05 heeft histonet-requ...@lists.utsouthwestern.edu histonet-requ...@lists.utsouthwestern.edu het volgende geschreven: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. PCR on paraffin sections (E. Wayne Johnson ???) -- Message: 1 Date: Mon, 24 Jun 2013 00:27:06 +0800 From: E. Wayne Johnson ??? e...@pigsqq.org Subject: [Histonet] PCR on paraffin sections To: histonet@lists.utsouthwestern.edu Message-ID: 51c721da.70...@pigsqq.org Content-Type: text/plain; charset=UTF-8; format=flowed Hi. We would like to start doing PCR on paraffin fixed tissues from pigs.. It has some appeal to us because we can see the lesions in the tissue and then we can go after the causative agent post-hoc. It may be useful in cases where we have clear lesions on histopath but not the right fresh tissues for PCR. We are very limited on what we can do with IHC because of the extreme difficulty of procuring the primary Ab. It's very simple to make PCR primers here. We do have PCR up and running for all of the diseases that we are interested in. I understand that the process is basically transferring the sections to a small tube instead of a slide, then dewax with a little xylene, and remove the xylene with alcohol, then digest the section to release the viral RNA. Does anyone have any recommendations, advice, or experience with the digestion step, and can suggest an appropriate enzyme mix for this digestion? Yes I am considering in-situ hybridization but we have working PCR methods so that seems to be a simple logical step. E. Wayne Johnson Enable Ag Tech Beijing. -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 115, Issue 26 * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Fatty Fixation
Try formalin-aceto-alcohol (F-A-A)...it works pretty well =) Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk Sent: Friday, June 21, 2013 3:19 PM To: White, Lisa M.; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Fatty Fixation What exactly is wrong with the fatty specimens? If the nuclei look smudgy, with no nuclear detail, then it has not been fixed long enough. If the fat is still in the tissue and you cannot section it on a microtome, then the tissue has not had enough time during processing, especially length of time in xylene and paraffin. So it would be a processing problem, not a fixation problem. And possibly a grossing problem, if the fatty tissue is grossed to thick for the length of time on the processor. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 Opinions expressed do not reflect on the hospital. -Original Message- From: White, Lisa M. Sent: Thursday, June 20, 2013 2:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fatty Fixation Does anyone have a method they will share to fix fatty specimens? Does anyone utilize a stir plate? Any help greatly appreciated. We currently use Alcoholic Formalin but the results are not reliable. Lisa White HT(ASCP) Supervisory HT James H. Quillen VAMC Corner of Veterans Way and Lamont VAMC Warehouse BLDG. 205 PO Box 4000 PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cholesterol
Has anyone ever done work with cholesterol receptors? Specifically APoB and E? Wondering if there is a specific IHC or special that will stain for these? I know Oil Red would stain all the fat, but they are wanting more of the affinity of each cell to uptake the cholesterol (ie the receptors). Can you get as specific as APoB or do you have to stay general as in the LDL type antibodies? Just throwing it out there to see if there is any insight while I google my day away on the subject =) Thanks in advance, and happy Thursday!! Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FAB fragments
I am going to try using a FAB fragment to block some mouse on mouse IHC staining...the protocol I am looking at says to dilute in PBS. My question is can I dilute it with my normal dilution buffer (DAKO, background reducing dilution buffer...which I'm sure is mostly PBS...), or should I using PBS? Thanks Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Cutting paraffin sections...on a cryostat?
Technically you are right...it's just a really cold tome. I guess you could cut sections with it...it would be super awkward though you're right. I wouldn't use normal tome oil on it because if you did decide to turn it back on, the normal oil will freeze up on you. Just continue to use the cryostat oil and you should be fine. Tell your colleague good luck!! Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Johnson, Kevin Sent: Friday, June 14, 2013 3:09 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Cutting paraffin sections...on a cryostat? Hi, all. A bit of an odd question: a colleague knows of someone wanting to cut paraffin sections who has a cryostat, but no microtome. Since a cryostat's basically a microtome in a freezer chamber, I thought that it may be awkward, but theoretically doable once it was brought to room temp and dried out thoroughly. However, I wondered if lubricants formulated for the cold might become too thin for use at room temp, possibly causing damage to moving parts. Any thoughts? Kevin Johnson University of Miami Diabetes Research Institute ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Extra Money
I already PRN at a local hospital, but that is usually only one or two shifts a month...I need something to add a third job to bring in extra dollars. I hope you weren't trying to act like I was being lazy or complaining Lee...as I am not either one...I have a new baby and the strain of putting her into daycare is A LOT!! Just trying to stay afloat. Thank you for everyone's legitimate suggestions. I will try a few of those. Thanks again! Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk Sent: Wednesday, May 29, 2013 5:10 PM To: Histonet Subject: Re: [Histonet] Extra Money I don't suppose you want to hear this, but several people are working extra jobs. Lee Wenk (not Peggy). -Original Message- From: Sarah Dysart Sent: Wednesday, May 29, 2013 5:05 PM To: Rene J Buesa ; joelle weaver ; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Extra Money I plan on doing a poster for NSH...does that count =) Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From: Rene J Buesa [mailto:rjbu...@yahoo.com] Sent: Wednesday, May 29, 2013 3:37 PM To: joelle weaver; Sarah Dysart; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Extra Money Not even that! If you publish you will build a better c.v. but that is not assurance tof being appreciated by any prospective employer. In the rare event that it is appreciated you will be able to negotiate a better salary, but that is all! René J. From: joelle weaver joellewea...@hotmail.com To: Sarah Dysart sdys...@mirnarx.com; histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Wednesday, May 29, 2013 4:31 PM Subject: RE: [Histonet] Extra Money Publish Joelle Weaver MAOM, HTL (ASCP) QIHC From: sdys...@mirnarx.commailto:sdys...@mirnarx.com To: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu Date: Wed, 29 May 2013 20:20:10 + Subject: [Histonet] Extra Money Anyone know any ideas on how to make a couple extra bucks a month using our histology-awesomeness?? Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Extra Money
Well if that's the case I just spent a lot of my companies money for nothing... Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: Morken, Timothy [mailto:timothy.mor...@ucsfmedctr.org] Sent: Thursday, May 30, 2013 10:53 AM To: joelle weaver; Sarah Dysart; Rene J Buesa; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Extra Money Actually it could be like earning money because I believe a poster presenter gets a free trip to the NSH meeting. Workshop presenters certainly do. Why do you think there are so many repeat presenters!?!? Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Thursday, May 30, 2013 8:37 AM To: Sarah Dysart; Rene J Buesa; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Extra Money Maybe to the nice folks at NSH :) Joelle Weaver MAOM, HTL (ASCP) QIHC From: sdys...@mirnarx.com To: rjbu...@yahoo.com; joellewea...@hotmail.com; histonet@lists.utsouthwestern.edu Date: Wed, 29 May 2013 21:05:02 + Subject: RE: [Histonet] Extra Money CC: I plan on doing a poster for NSH...does that count =) Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From: Rene J Buesa [mailto:rjbu...@yahoo.com] Sent: Wednesday, May 29, 2013 3:37 PM To: joelle weaver; Sarah Dysart; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Extra Money Not even that! If you publish you will build a better c.v. but that is not assurance tof being appreciated by any prospective employer. In the rare event that it is appreciated you will be able to negotiate a better salary, but that is all! René J. From: joelle weaver joellewea...@hotmail.com To: Sarah Dysart sdys...@mirnarx.com; histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Wednesday, May 29, 2013 4:31 PM Subject: RE: [Histonet] Extra Money Publish Joelle Weaver MAOM, HTL (ASCP) QIHC From: sdys...@mirnarx.commailto:sdys...@mirnarx.com To: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthweste rn.edu Date: Wed, 29 May 2013 20:20:10 + Subject: [Histonet] Extra Money Anyone know any ideas on how to make a couple extra bucks a month using our histology-awesomeness?? Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthweste rn.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern .edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Extra Money
Anyone know any ideas on how to make a couple extra bucks a month using our histology-awesomeness?? Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Extra Money
I plan on doing a poster for NSH...does that count =) Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 From: Rene J Buesa [mailto:rjbu...@yahoo.com] Sent: Wednesday, May 29, 2013 3:37 PM To: joelle weaver; Sarah Dysart; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Extra Money Not even that! If you publish you will build a better c.v. but that is not assurance tof being appreciated by any prospective employer. In the rare event that it is appreciated you will be able to negotiate a better salary, but that is all! René J. From: joelle weaver joellewea...@hotmail.com To: Sarah Dysart sdys...@mirnarx.com; histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Wednesday, May 29, 2013 4:31 PM Subject: RE: [Histonet] Extra Money Publish Joelle Weaver MAOM, HTL (ASCP) QIHC From: sdys...@mirnarx.commailto:sdys...@mirnarx.com To: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu Date: Wed, 29 May 2013 20:20:10 + Subject: [Histonet] Extra Money Anyone know any ideas on how to make a couple extra bucks a month using our histology-awesomeness?? Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Gross Room cleaner
Bleach =) Just make sure to rinse after cleaning or they will rust Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, May 14, 2013 11:29 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Gross Room cleaner What do you guys use to clean your Gross Room utensils? I bought Lem'in Lift from Mantek in the past to soak the rulers, scissors, tweezers, etc...Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Test
Have there really been no emails in 2 days?? We just upgraded our email and I want to make sure I didn't lose this list... Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Eosin not working for xylene free HE
This exact reason is how I convinced my lab to go back to xylene!! I figure it's because there is water in your substitute. Because you can't see if there is water in it (from the air moisture or wherever...) it becomes frusterating and annoying to have to change out to fresh solutions sometimes several times a day!! Good Luck!! Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gerard Spoelstra Sent: Wednesday, March 27, 2013 9:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin not working for xylene free HE Hi everyone, I have not had any success optimising the eosin counterstain in the absence of xylene. We routinely use 0.5% eosin Y in 95% ethanol. I've tried taking sections through to this to absolute ethanol but then air drying , but it leaves blotches of eosin. Using eosin 0.5% aqueous with a few drops of acetic acid doesn't appear to work, the keratin stains heavily but not elastin. After washing in water I leave for a short time in the oven then at room temperature for 5 minutes then coverslip with the dako atomatic cover slipper. For all the laboratories that have gone xylene free, are you just putting up with less than optimal eosin staining? Gerard Spoelstra Murdoch University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Citadel
Thank god this thing has finally DIED!!! I need to order used equipment from a Texas source (sorry Denise...I would love to order from you again, but I have to order from a Texas company...). Does anyone know of a good used equipment company in Texas that sells processors? Thanks Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Maxvison anti-Rabbit HRP Antibody Substitution
The Biocare polymers (MACH2 series) are excellent!! Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Suresch, Donna L. Sent: Monday, February 25, 2013 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Maxvison anti-Rabbit HRP Antibody Substitution Hello All, I have been using Maxvision anti-Rabbit HRP secondary antibody with excellent results. The product has been discontinued. Has anyone found a good substitution for this antibody? Thank you. Donna L. Suresch Senior Imaging Research Scientist Merck Research Laboratories Department of Imaging - West Point Campus Mail Stop: WP44KOffice: WP44-H129 770 Sumneytown Pike PO Box 4 West Point, PA 19486-0004 Phone: 215-652-7349 Fax: 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Type I Water
I put tap water in my water bath and haven't had issues. Alternatively you could use DI water which would be a little cleaner... Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Friday, February 15, 2013 8:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Type I Water Clearly I sent this too quickly (early) - this is in regards to the water in our tissue float baths - does it HAVE to be Type I? Thanks Nancy Good Morning- I feel like I am racking up frequent flier miles on Histonet lately:) We are seeing bacteria in our type I. This requires us to obtain our Type I from another of our lab sites. Do I have any other options? Like tap water? Nancy Schmitt HT, MLT(ASCP) NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PRN pay?
How much is the standard pay for PRN in Texas? IHC, specials, and routine to be included in the work. Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cytology
Sodo you guys think it would be ok to leave cytospins in Penfix overnight in the fridge (4C), or would it degrade the cells? I'm doing Penfix instead of 95% because I need the formalin linkage on the cells... Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Xylene Free Processing?
FYI, I've tried ClearRite, and while I guess it technically works...it's a giant pain to work with because you have to extend out your times. I also found that it screws with Eosin because if it gets moisture in it (which you can't see); the water bleaches out the Eosin. Just my two cents worth =) Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, January 09, 2013 2:46 PM To: Jones, Laura; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Xylene Free Processing? Please go to http://www.histosearch.com/rene.html and you will find my articles on the sibject. René J. From: Jones, Laura lpjo...@srhs-pa.org To: Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu Sent: Wednesday, January 9, 2013 2:37 PM Subject: [Histonet] Xylene Free Processing? I would appreciate hearing all of your expert opinions on processing tissue without the use of xylene or any type of xylene substitute. Our processor was down recently, and a friendly local lab processed our tissue this way for us. Of course, the Pathologists loved it. The process was formalin, followed by graduated percentages of only isopropyl alcohol, and then paraffin. What are your feelings concerning immunos? Overall cost? Any other thoughts? Thanks in advance! Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] vacuum sealer
Get one from Walmart. Half the price and last just as long =) Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernadette del Rosario Sent: Sunday, December 30, 2012 9:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] vacuum sealer Hi histonets..Im looking for a good brand of vacuum sealers for storing histology tissue samples.Dont you have any issues with this sealers.Im looking for one which retains a little formalin in it.I want to know which type you are using..Thanks a lot.Have a great day.. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Pathologist Consult
Does anyone know of any good services that do a per slide pathology read out? I would prefer a veterinary pathologist and it would not be a very large case load. Mainly tox. related reports would be what we need generated. Thanks Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Eosin
Buy one with Phloxine in it. It will make it more red and stronger... Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Thursday, August 09, 2012 10:23 AM To: histonet Subject: [Histonet] Eosin Hi Folks...I am hoping you all give me a little help. Our Pathologists are complaining about our Eosin on the HE's being weak. The funny thing is, is that it can go one day to the next...one day it looks great...the next it is weak!! I have already done some experimenting with...1) time tissue spends in Eosin 2) making sure that the alcohols after Eosin are the proper concentrations3) reducing the time that the tissues spends in the alcohols atfer Eosin... I have even gone as far as 4) increasing the rinse time in water after the decolorizing and bluing steps. 5) I have checked the pH of the water as well. Any help and suggestions would greatly appreciated!! Thanks Gang!! Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.han...@parrishmed.com * = This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you = ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Teabags
I use hair perm papers that I buy from a local beauty supply store. WAY cheaper, and work very well! Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Tuesday, August 07, 2012 2:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Teabags Susan Walzer notes I was also tired of digging bone marrow particles and biopsies out of the stitching. Some people like [teabags] because they can just dump tissue in them but they do not have to fight with them when embedding. Biopsy cassettes can trap air and float. The best all around product is Obex round papers. For people who like to dump you can fold them into cones and use like filter paper. They are the best thing for all around protection of small and friable tissue. I'm not familiar with Obex round papers. See http://histowrap.com/ for more information. Bob Richmond Samurai Pathologist Asbury Place, a continuing care retirement community in Maryville TN, about half an hour south of Knoxville (but I have no plans to retire!) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Control Blocks
Hello world... I am looking for a human colon cancer control BLOCK. I know I can get slides everywhere, but I need the block (that I can keep forever). Any ideas?? Thanks Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Contract rate
Hola peeps!! So does anyone know what the going rate for after hours contact HT work is. We are an urban research lab and it would be after hours work. We are in Austin Texas if that helps any =) Thanks Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RRAS IHC
Has anyone ever done any staining with RRAS. I tried doing this about a year ago and thought it had been swept under the rug...nope...I'm trying to find a good positive control...and possibly a picture of it? Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Non-Histo. question
So, I go on maternity leave in 52 (I hope...) days...while out I will have one of those automatic messages that says I'm out...Since I get say 20ish emails a day from histonet I would assume that 20ish of these replys will go out to everyone, and I don't want to drive people bonkers. Maybe the moderator could answer...what do you want me to do? Cancel and rejoin when I get back, or is this just something that gets filtered out? Thanks Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Hold IHC overnight
Just found out I have to go to a meeting in 2 hours for the rest of the day (I haven't even eaten lunch yet). I do IHC manually, they just got done cooling off from HIER...I can store them overnight in buffer right? Just double checking myself as this run is an antibody validation run... -S- Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Conferences?
Does anyone know of any conferences or conventions going on in the histology world in the next 8 weeks?? I am 8 months pregnant and was just told that I need to go to a conference this year...unfortunately I cannot fly. The conference would have to be somewhere drive-able from Austin, Texas (preferably no more than 10 hours or so...we Texas people are used to long drives to get places...). That would open up LA, OK, TX, MO, etc. Thanks! Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Eosin
So I have always used Eosin-Y stain...can someone tell me which is better...alcohol or phloxine?? Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Muscle Biopsy Procedure
I have done the same thing with acetone instead of alcohol... Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Donadio Sent: Monday, June 25, 2012 10:33 AM To: Bea DeBrosse-Serra; 'Ian R Bernard'; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Muscle Biopsy Procedure One place I was at didnt get many muscle biopsies so we didnt keep liquid nitrogen and isopentane, which is what I was trained to use initially. Here's what we did when we got a muscle biopsy and it worked fine. After cutting the biopsy into 3-4 mm portions, we wrapped it in aluminum foil and snap froze it in a slush made from 100% alcohol and dry ice. We then shipped it in a styrofoam lined box full of peices of dry ice. The dry ice was made into small peices so we could place the snap frozen biopsy in the middle. Good luck! From: Bea DeBrosse-Serra bdebrosse-se...@isisph.com To: 'Ian R Bernard' ibern...@uab.edu; histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Monday, June 25, 2012 10:16 AM Subject: [Histonet] RE: Muscle Biopsy Procedure Ian, Check with Children's Hospital in Cincinnati. The Pathology Department there performs a lot of muscle biopsy procedures and gets a lot of outside biopsies as well. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ian R Bernard Sent: Friday, June 22, 2012 7:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Muscle Biopisy Procedure Our lab never had the opportunity to perform a muscle biopsy procedure until now: we have a request for one. Since we never did one, we don't have a written procedure. We are looking for a benchmark or proven written procedure to freeze and ship the specimen to the Joint Pathology Center in Washington DC for processing. We will not perform any other aspects of the procedure, just the freeze and ship. Hope to do this next week. Any assistance is greatly appreciated. Ian R. Bernard Ian R. Bernard, MSHA, HT (ASCP) 10th Medical Group- Anatomic Pathology Lab USAF Academy, CO 80840 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] paraffin melting in VIP
This is probably a best case scenario, but for labs like the one I am currently in I melt it in the processor. My lab doesn't have the funds to buy me a melting pot (I have had them in all my other labs, just don't know what they are technically called). To answer your question it usually takes overnight and it's melted, but sometimes I end up having to add a little more to get to top off level, that takes a couple hours. Good Luck!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, May 11, 2012 1:28 PM To: histonet@lists.utsouthwestern.edu; gu.l...@gmx.at Subject: Re: [Histonet] paraffin melting in VIP Regardless of the time it takes or of how many people do it, melting the paraffin directly in the VIP should not be done because it causes the heating elements to work extra reducing their useful life. They are quite expensive to replace!. Melt the paraffin outside the VIP and use the VIP only to keep the melted paraffin at the temperature you desire. René J. --- On Fri, 5/11/12, Gudrun Lang gu.l...@gmx.at wrote: From: Gudrun Lang gu.l...@gmx.at Subject: [Histonet] paraffin melting in VIP To: histonet@lists.utsouthwestern.edu Date: Friday, May 11, 2012, 1:58 PM HI! A question for those, who melt the paraffin directly in the VIP. How long does it take to melt the pellets in the VIP-oven? Thanks Gudrun ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Batch Controls
I still use batch controls, but I am one of the few left that is not automated. I do everything by hand. I think you are right though, placing a control tissue on each slide is the only way to be sure that everything was dispensed correctly...especially if you are using a Ventana... Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Glen Dawson Sent: Thursday, April 19, 2012 9:20 AM To: histonet Subject: RE: [Histonet] Batch Controls All, I place a positive control on each slide, next to the patient tissue for all of the reasons already mentioned, but we are missing the obvious one. Many of us use some kind of automated immunostainer where there is no gaurantee that, because the CD3 in position #4 (batch control) worked, the CD3's loaded in positions 6, 9, 13, and 21 also worked. Perhaps a reagent ran out or there was air in a line for part of the process for any one of these other CD3's and, because there is no control on the same slide, there may be a false negative result reported due to the use of a batch control. For this reason alone, one should think hard about using batch controls. Just My Opinion, Glen Dawson BS, HT(ASCP) QIHC Histology Technical Specialist Janesville, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] OCT embedded frozen tissue for paraffin embedding
Yes, just wash out the OCT and fix as normal...Just remember that these tissues might not look the same as normal fixed tissues because they were frozen first. Sometimes you can get cracking or freezing artifact. Good Luck =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Tuesday, January 24, 2012 1:51 PM To: histonet Subject: Re: [Histonet] OCT embedded frozen tissue for paraffin embedding One of our investigators wants to take his frozen tissue (embedded in OCT) and now fix in formalin for paraffin embedding. I assume he needs to wash out the OCT (?phosphate buffer that formalin is prepared in) and then fix in 10% formalin. I would appreciate feedback from the listers. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Xylene and Preggers
Hey all, so 2 things... A. Does anyone have anything saying that .75% (yes less than 1) is an acceptable exposure limit for a pregnant person and B. Does HR have the right to tell people that you are pregnant after you ask them questions (ie. Your manager, and all the way up??) Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Caspase3
For all of you research guys out there working with this. I was asked to pose the question of how you quantify this stain. I know histology is a black and white yes/no kind of science, but I am being asked to quantify this stain. Are just the very positive cells counted as positive? There is what appears to be non-specific staining on some of the tumors (not all of them). This non-specific staining is only in the tumor and not in the surrounding normal tissue. Is this a sometimes artifact of the stain? For Ki-67 we are quantifying the stain using an average of positive cells per area. This gives you a sort of index to determine how Ki-67 positive each tumor is. The problem with the Caspase3 is that this fuzzy stain is in 2 of our tumors, and while I say read through it (non-specific staining) others are thinking it means something. Anyhoo, any help would be greatly appreciated. Basically how do you report your Caspase results? Just as a positive or negative (which every tumor will be positive) or what other method do you use. Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Amazing
I find it amazing sometimes when you don't do something for awhile how quickly your brain throws the information away. That being said...I know back in the day when I was learning histology we used to make our own acid alcohol solution (now where I am had a butt load of Clearifier so I was using that up). I don't want to buy that stuff anymore as making the solution is way cheaper and works just as well. I want to say it was like a 1% acid solution in alcohol?? What was the acid? For some reason my brain says glacial acetic...but time has made me forget. Is the alcohol you mix it in 100% or something lower with a water content to it? Please help my alzheimers =) Happy Thursday!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Look at me...
I'm just full of questions today!! This one is IHC...I have been trying to optimize a Caspase3 stain for several months now and it is still just chalked full of background gradoo. I do all the blocking including Fc receptors...still junk. The clone I have been using is, from abcam (ab2302). I don't see the specific clone name listed. I am staining human xenografts raised in mouse. I get a whole lot of background staining making it very hard to find the positive staining. The recommended dilution is about 1:30, but I have diluted all the way up to 1:500. At the higher dilution no positive staining or background is observed. Does anyone know of a good Caspase3 antibody, preferably mouse monoclonal? All the rabbit polyclonal antibodies are difficult to stain on these xenografts. Thanks again =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Respirators and Routine Histology
I'm in Texas and have been in histology labs since 1998 in 4 different places. None of them did we ever wear a respirator for normal histology work. We did have to wear masks when cutting frozens because of possible TB in lung tissue, but that was it. Most labs should have some kind of negative pressure in them and be exhausting out of the room. I also have always had some kind of hood over the staining station, and usually another hood to gross specimens in. I would say wearing a respirator every day for 8 hours would have made me decide on another field!! How irritating!! Good Luck! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jsjurc...@comcast.net Sent: Thursday, January 05, 2012 2:31 PM To: Linda Blazek Cc: histonet@lists.utsouthwestern.edu; Amy Self Subject: Re: [Histonet] Respirators and Routine Histology They must be in a liberal part of Michigan. - Original Message - From: Linda Blazek lbla...@digestivespecialists.com To: Lisa Brenner li...@hollandhospital.org, Amy Self as...@georgetownhospitalsystem.org, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Thursday, January 5, 2012 2:30:20 PM Subject: RE: [Histonet] Respirators and Routine Histology Nothing at all??? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lisa Brenner Sent: Thursday, January 05, 2012 3:22 PM To: Amy Self; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Respirators and Routine Histology No, not here. We don't wear anything at all. We have excellent air flow, and we wear the badges that test for exposure to xylene and formalin annually which I think is a CAP requirement. Our results have been better than acceptable every year. Lisa Brenner HTL (ASCP) Histology Technical Consultant Holland Hospital phone: (616)394-3184 li...@hollandhospital.org Amy Self as...@georgetownhospitalsystem.org 1/5/2012 3:09 PM Happy New Year to All, I need some help from all of you out there in histoland. How many of you wear respirators during your entire 8 hour work day for routine histology? If you don't wear a respirator do you wear any type of mask or shield at all for routine histology? Also if any of you have any histology safety procedures or information that you would be willing to share with me I would greatly appreciate it. Thanks in advance for all of your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: The information contained in this e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information, or Protected Health Information as such term is defined under the Health Insurance Portability and Accountability Act of 1996 (HIPAA). Any unauthorized review, use, disclosure, copying or distribution is prohibited and may be unlawful. If you believe you have received this e-mail in error, please contact the sender by reply e-mail and delete all copies of the original message, including attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Filter Paper
Hey guys, What is the standard filter paper for hematoxylin. I threw away the box so now I don't know the grade...oops Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ahh...
So I have a bunch of samples I processed last night and looks like I am not going to be able to embed them today. They are mouse samples, so smaller than others. Would it be best just to pull them out of the processor (they are currently in hot paraffin) let them solidify at room temp, and then tomorrow just put them in the embedder to remelt? Or should something else be done to preserve them. Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] standard operating procedure HE
Usually now I only run an HE control when I get a new lot of hematoxylin or eosin to make sure the stain is good. In other labs (especially if they are a strict GLP lab) I have run a control daily. I think it is just dependent on how strict your QA department is. Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Corrie Vernick Sent: Wednesday, November 23, 2011 8:52 AM To: Histonet Lists Help Subject: [Histonet] standard operating procedure HE Hi, I am a student and I am working on an assignment writing a mock SOP for the H E staining procedure, both progressive and regressive. One heading of the SOP is control. I read the book and tried to google for a good answer as to what this part of the SOP entails. I know that controls are run to make sure that the slides are staining properly before new reagents are used on patient tissue. I am wondering if what I should be putting under the control heading of the SOP is that a control should be run after every stain or reagent change, or if there is something I am missing. Thank you, Corrinne Vernick Keiser University Orlando, FL U.S.A. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC
So...I am doing IHC stains with Caspase3 and Ki-67. Does anyone know a positive control for both of these at the same time? Will tonsil pop positive for both?? If not, what are good controls for each of them? Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] decal question
Formalin? Isn't all decal acid decal?
Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Monday, October 03, 2011 11:32 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] decal question
Hi Everyone,
I have a new student taking course work at UND and using my lab for her
practical clinical site. She took a test on decal today and there was a
question we didn't know the answer to.
What fixative should not be used for acid decalcification? Would it be
osmium tetroxide?
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email mailto:pru...@ihctech.net pru...@ihctech.net
web site http://www.ihctech.net www.ihctech.net
This email is confidential and intended solely for the use of the Person(s)
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RE: [Histonet] Alignment tool
I actually just got a flyer for one of those from American Mastertech that looked pretty neat? http://www.americanmastertech.com/store/main.aspx?p=ItemDetailStylesitem=EQS05 Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jsjurc...@comcast.net Sent: Friday, September 30, 2011 9:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Alignment tool Who sells the microtome alignment tool that clamps onto the base of the microtome and also into the cassette holder to assure that all microtomes in the lab are in the same cutting plane? Do the bubble ones work as well? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
