[Histonet] Well. Don't I look like a dummy!
Sorry that it took me 3 emails to get the link right. Licking Memorial Hospital has an opening for a full time certified histology tech. Dayshift position, 7:30am to 4:00pm, Monday through Friday with no weekends or holidays. If interested check us out and apply online at: http://www.lmhealth.org/ Tom Mc Nemar, HT(ASCP) Histology Supervisor (740) 348-4163 Licking Memorial Hospital 1320 West Main Street Newark, OH 43055 This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology position in Newark, Ohio
Sorry. Not sure why the link didn't work. www.lmhealth.orghttp://www.lmhealth.org Tom Mc Nemar, HT(ASCP) Histology Supervisor (740) 348-4163 Licking Memorial Hospital 1320 West Main Street Newark, OH 43055 This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology position in Newark, Ohio (approximately 30 miles east of Columbus)
Hello all, Licking Memorial Hospital has an opening for a full time certified histology tech. Dayshift position, 7:30am to 4:00pm, Monday through Friday with no weekends or holidays. If interested check us out and apply online at www.lmhealth.orghttp://www.lmhealth.org. Tom Mc Nemar, HT(ASCP) Histology Supervisor (740) 348-4163 Licking Memorial Hospital 1320 West Main Street Newark, OH 43055 This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Cassette Labeler
We use General Data for cassettes and slide labels and are very happy with it. We are adding Cytology to it this year. Tom Mc Nemar, HT(ASCP) Histology Supervisor (740) 348-4163 Licking Memorial Hospital 1320 West Main Street Newark, OH 43055 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Debbie Granato Sent: Monday, January 26, 2015 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette Labeler Our lab is currently looking into purchasing a cassette and slide labeler. We are a small lab and are looking for a stand- alone unit with the flexibility to be used with the computer or bar code scanner at a later date. I would appreciate any feedback or suggestions for any model that may fulfill our needs. Thank you, Debbie Granato HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Electronic monitoring systems
We are tied into the main lab system. Rees Scientific for freezers, refrigerators, and VIPs. Tom Mc Nemar, HT(ASCP) Histology Supervisor (740) 348-4163 Licking Memorial Hospital 1320 West Main Street Newark, OH 43055 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael Ann Jones Sent: Tuesday, January 20, 2015 2:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Electronic monitoring systems Hi all, I was wondering - a little while ago there was mention on the blog of equipment (processors) being monitored and alerting the 'manager' if they went down. I'm sorry I cannot remember what system this was. What do you all use? Also, what temperature control monitor is everyone using? Ours has flaws and we are gathering information. Can the two systems be combined under one company? Any help is appreciated. Thank you! Michael Ann Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.commailto:mjo...@metropath.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Snomed
We haven't used snomed codes for many years. Tom Mc Nemar, HT(ASCP) Histology Supervisor (740) 348-4163 Licking Memorial Hospital 1320 West Main Street Newark, OH 43055 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jessica.va...@hcahealthcare.com Sent: Wednesday, November 19, 2014 2:35 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Snomed How many of your pathologists, LIS or others are using Snomed? Jessica Vacca HCA Epic Anatomic Pathology Application Lead Clinical Services Group 2545 Park Plaza Bldg III Nashville, TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: HISTO/CYTO SKILL REVIEW
We are JCAHO inspected (just this year) and only do yearly competencies. Tom Mc Nemar, HT(ASCP) Histology Supervisor (740) 348-4163 Licking Memorial Hospital 1320 West Main Street Newark, OH 43055 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of rmweber...@comcast.net Sent: Thursday, November 06, 2014 9:56 AM To: histonet Subject: [Histonet] HISTO/CYTO SKILL REVIEW Hi, I was wondering if anyone knows how often Joint Commission requires cytologist and histologist to have their competency reviewed. I'm not talking about their job performance review, but how often to review their job skills with a check list under direct observation. We have been doing it quarterly for histologists. Thanks so much, Marilynn Weber H.T.(ASCP)QIHC Coastal Pathology Consulting Services LLC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Cold plates for icing blocks?
Check out the Histo-cool available from Market Lab or Mercedes Medical (and probably others). We use the small ones at each cutting station and they work great. You can pour a little water in and it stays cold all day. http://www.marketlab.com/histo-cool/p/Histo-Cool/ Tom Mc Nemar, HT(ASCP) Histology Supervisor (740) 348-4163 Licking Memorial Hospital 1320 West Main Street Newark, OH 43055 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Monday, November 03, 2014 2:29 PM To: Histonet Subject: [Histonet] Cold plates for icing blocks? Does anyone use a cold plate, like that used for embedding, for icing blocks for sectioning? Just an idea Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.mor...@ucsfmedctr.orgmailto:tim.mor...@ucsfmedctr.org CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Combined Case prefix
We have a few different prefixes. We use Meditech and have different enter/edit screens depending on whether the specimen is a cytology or surgical. It also help in searches and gathering statistics if you can limit to a specific prefix. Tom Mc Nemar, HT(ASCP) Histology Supervisor (740) 348-4163 Licking Memorial Hospital 1320 West Main Street Newark, OH 43055 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jessica.va...@hcahealthcare.com Sent: Tuesday, October 28, 2014 10:37 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Combined Case prefix How many facilities have combined their prefixes? Meaning your pathology and cytology are using the same prefix so there is no difference when accessioning cases of pathology or cytology. What do you feel are the pro's and con's of this? Jessica Vacca ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Looking for suggestions, ideas
We did this a few years ago for our local coroner's office. They send us their tissues and we process them and cut the slides. We return the slides and blocks to them so there is nothing in our files. Are you returning the blocks with the slides? Pretty simple to do. I created a new specimen prefix in Meditech to differentiate them from our in-house cases and built a new charge procedure that has a zero charge for the professional component. The coroner does the dissection and places the tissues into the cassettes. The cassettes are a different color and their numbering system is unique so there's no problem identifying their cassettes. I assume that you will be placing the tissues into the cassettes on-site so I think that a different color cassette should solve your identification problem. You may want to think about also using a matching slide color to make it even simpler. Tom Mc Nemar, HT(ASCP) Histology Supervisor (740) 348-4163 Licking Memorial Hospital 1320 West Main Street Newark, OH 43055 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Tuesday, August 26, 2014 4:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Looking for suggestions, ideas Hi Folks, Here is our situation. In the not too distant future we will be receiving additional specimens from 3 dermatologists. What they need is to have these specimens processed, embedded, cut and H/E stained. Once we've got the slides coverslipped they are to be sent back the dermatologists. We are not doing the interpretation. What I'm looking for is an idea about getting these into our LIS (PowerPath) and designating them for return to this group. We should be able to capture the TC and need to account for the workload. I'm thinking we could prefix them differently at the time of accessioning and then create something in the LIS attached to that designation which only generates a TC. I'm wondering if anyone else does anything like this? I'm also interested in having this differing designation for efficient workflow. I don't want any of these making their way to our pathologists. I've thought of identification by color of block and a new prefix. Just not sure if/what others are doing, how difficult to implement for an LIS standpoint (separation of TC and PC) and any other considerations. Thanks, I know I've got all the superior minds out there. Your thoughts are greatly appreciated. Tom Jasper Thomas Jasper HT (ASCP) BAS AP/CP Supervisor Deaconess Hospital 600 Mary Street Evansville, IN 47747 thomas.jas...@deaconess.com 812-450-2485 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: biopsy bags for processing - alternatives
Personally, I prefer the biopsy bags and find them easy to work with. I generally hold the bag in one hand (allows the paraffin to cool enough to hold the tissue) and use forceps to slowly open it. If it is an ECC or something similar, I can wrap the opened bag around a couple of fingers and use a small spatula to scrap. With a little practice, I have found it to be quick and efficient. Tom McNemar, HT(ASCP) Histology Supervisor Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, August 13, 2014 11:24 AM To: Histonet Subject: [Histonet] biopsy bags for processing - alternatives All knowing Histonet, Our grossing staff uses nylon biopsy bags to enclose some biopsy specimens. The embedding staff find them troublesome because when they pull the bags open they tend to pop open and throw the tissue off in all directions. They have to be very careful opening these. Is there another bag made of some other material that is less prone to this problem? For various reasons some of these samples can't be put on sponges. They do wrap some in flat biopsy paper, but not others. It seems to be a grossing personal preference more than anything else. Thanks for any and all info! Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.mor...@ucsfmedctr.orgmailto:tim.mor...@ucsfmedctr.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: G.I. biopsies and special stains
H. Pylori by IHC on all stomach biopsies and Alcian Blue on all esophageal biopsies done automatically. Tom McNemar, HT(ASCP) Histology Supervisor Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kathy.mach...@lpnt.net Sent: Monday, June 09, 2014 9:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] G.I. biopsies and special stains I was wondering if other labs are automatically ordering special stains and/or IHC on stomach biopsies and esophageal biopsies? Or do you wait until HE is screened? Thanks! Danville Regional Medical Center Danville, VA kathy.mach...@lpnt.netmailto:kathy.mach...@lpnt.net 434-799-3868 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Xylene substitute for processing GI biopsies
We have used Americlear for many, many years. Processing and staining of everything (not just GI). It's a d-limonene product so it does have an orangish smell. I have always had VIPs and we have never had a problem. Tom McNemar, HT(ASCP) Histology Supervisor Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Friday, April 18, 2014 11:40 AM To: Lori Gemeinhardt; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Xylene substitute for processing GI biopsies Lori, We use Formula 83 from CBG for both processing and staining. We have used this for years with no problem for our GI biopsies. It wouldn't do any good to share my processing schedule with you though since we have a different processor. If you want to talk or any further information feel free to contact me. We also recycle our Formula 83. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lbla...@digestivespecialists.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, April 18, 2014 11:15 AM To: Lori Gemeinhardt; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Xylene substitute for processing GI biopsies Hi Lori: Instead of xylene you can use isopropyl (also known as 2-propanol and propyl alcohol) and after sectioning you can use dishwasher soap before going directly to the staining procedure. After staining you need nothing but an oven to dry out the stained sections and go diractly to coverslipping. If you want details on the above please go to: http://www.histosearch.com/rene.html and read my articles on the subject, including processing protocols. If you follow the instructions, you will have a totally xylene free lab. René J. On Thursday, April 17, 2014 8:19 PM, Lori Gemeinhardt loreli...@mac.com wrote: Hi there! I have spent a lot of time reading the archives, but still need some specific and current input/advice. I'm interested in overnight processing for GI biopsies on a Tissue-Tek VIP with a xylene substitute. I need insight on protocols, brands, effects on staining. The good, the bad, the ugly. I have only used xylene in my many years of experience! This is completely new (to me), and rather overwhelming. It would be ideal to use the same substitute in the staining process, so any consideration with that is also appreciated. I understand this has been discussed, at length in the past, and I apologize I am just hoping to zero in on the best case scenario as a starting point. Thanks, in advance, for your help! Lori ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Body Release and Cleanin in Morgue
We stopped doing autopsies years ago. Since then, we have transferred ownership of the morgue to Nursing Services and have nothing to do with it. Before all that it was the deiner who was responsible for releasing the bodies and saw that it was cleaned by environmental services after each autopsy. Tom McNemar, HT(ASCP) Histology Supervisor Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Wednesday, March 26, 2014 5:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Body Release and Cleanin in Morgue Hello to all in histo-land. Who takes care of releasing the body to the funeral home and who does the cleaning of the seals and the molds on the drawers for the body storage? Is lab some how involved in this process? Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-2148 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Turn Around Time
Our benchmark is 24 hour TAT for 80% of cases. We provide same-day service for recuts and most in-house special stains. Tom McNemar, HT(ASCP) Histology Supervisor Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dawn Bugge Sent: Monday, February 03, 2014 12:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Turn Around Time Hello Histonet I am just curious what the standard for Turn Around Time is for most labs. I think a two day turn around time from the time the biopsy gets to the lab to the time the pathologist signs out a case is pretty fast. Thanks for your input. -- Dawn R Bugge Seattle Histology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Tissue-Tek TEC5
No. I have had 3 Tissue Tek embedding stations and never felt it was cold enough. It is in such close proximity to all that heat that I just assume that it is what it is. Other than that, no problems. I have found them to be very reliable. Tom McNemar, HT(ASCP) Histology Supervisor Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gregoire, Rhonda (MAFRI) Sent: Tuesday, January 21, 2014 1:09 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Tissue-Tek TEC5 We are looking to purchase a new embedding centre. If you are using the Tissue-Tek embedding centre do you find that the cold spot is cold enough? Any other pros/cons? Thanks Rhonda Gregoire, MLT Supervisor, Clinical Pathology Veterinary Diagnostic Services Manitoba Agriculture, Food and Rural Development 545 University Crescent Winnipeg, MB R3T 5S6 phone 204-945-7641 fax 204-945-7646 email rhonda.grego...@gov.mb.camailto:rhonda.grego...@gov.mb.ca ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] FW:
I am very happy with ours. Our system is from General Data. Specimen container labels are printed at accessioning, that label is scanned at grossing and cassettes are printed. Those cassettes are then scanned at the microtome and slide labels for that block only are printed from a small printer located at the microtome. We were unable to interface with our LIS (Meditech) so as a workaround, we have to print a report that sends the information over to the General Data system. There were some issues early on but it has proven to be a good system. I will be expanding it to include Cytology later this year. Tom McNemar, HT(ASCP) Histology Supervisor Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Burrage, Shannon L. Sent: Tuesday, December 31, 2013 9:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: From: Burrage, Shannon L. Sent: Tuesday, December 31, 2013 8:10 AM To: ,utsouthwestern.edu Subject: Hi: I'm new to this website, and anxious to learn from all. At this point, i'm wondering if anyone could give me any information on the general use of bar coding in histology. Such as available vendors, and necessary equipment, mostly your personal experiences with this system. Thanks, Shannon shannon.burr...@unitypoint.orgmailto:shannon.burr...@unitypoint.org This message and accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. §§ 2510-2521, and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Staining on Alcohol Fixed Smears
Thanks for the clarification. I appreciate the info! Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Cox Sent: Monday, December 16, 2013 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Staining on Alcohol Fixed Smears I agree that with no formalin exposure, there is no crosslinking that needs to be broken, but a then, do nothing approach to antigen retrieval oversimplifies the issue. Alcohol fixation makes changes in the protiens/antibodies also, although different changes than formalin does, and these changes need to be accounted for. In my research on alcohol fixed smears, I found that most antibodies perform best with some antigen retrieval, often gentler than formalin tissues require. Beth Cox, HTL/SCT(ASCP)QIHC Vagabond Histotech -- Message: 4 Date: Mon, 16 Dec 2013 11:13:59 -0500 From: Tom McNemartmcne...@lmhealth.org Subject: RE: [Histonet] Re: Staining on Alcohol Fixed Smears To: 'Beth Cox'bethc...@gmail.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu,levi.fr...@gmail.com levi.fr...@gmail.com Message-ID: E9A90E28259D2F4E84308C5E8EA8F7B4016951E94252@lmhs-exchange Content-Type: text/plain; charset=us-ascii Since there is no exposure to formalin, we do not do antigen retrieval on alcohol fixed specimens. We use the Ventana Benchmark XT and copy the protocol to a new number and use the Wet option rather than paraffin. No retrieval needed. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From:histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Cox Sent: Saturday, December 14, 2013 6:30 PM To:histonet@lists.utsouthwestern.edu;levi.fr...@gmail.com Subject: [Histonet] Re: Staining on Alcohol Fixed Smears Levi, I've worked up most of the more common antibodies on alcohol fixed smears. There are two things to remember here: 1) your specimens are alcohol fixed, meaning antigen retrieval needs are different, and 2) they are smears, meaning the cells weren't 'cut open' during microtomy, which impeeds some nuclear staining. Those are two different issues to consider. Some rules of thumb: 1) most cell membrane antigens will stain nicely with minimal changes to the protocols you use for FFPE specimens 2) most cytokeratin antigens stain nicely with little change to your FFPE protocols (if you need to change, start by cutting your antigen retrieval time in half) 3) most nuclear antigens require significant changes from FFPE protocols, and some are essentially impossible to stain using standard antibodies. When I return to work on Monday, I will forward you my protocol 'adjustments' for the antibodies you requested. Beth Cox, HTL/SCT(ASCP)QIHC Vagabond Histotech On 12/14/2013 12:00 PM,histonet-requ...@lists.utsouthwestern.edu wrote: Message: 4 Date: Sat, 14 Dec 2013 18:25:08 +0200 From: Levi Friedlevi.fr...@gmail.com Subject: [Histonet] Staining on Alcohol Fixed Smears. To:histonet@lists.utsouthwestern.edu Message-ID: CA+puqxTXL85pEwvR=sqPQA36tMJPm=sx2kv3gyebrbu0he3...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Hi Everyone. I am looking to stain alcohol fixed smears with a group of antibodies. The antibodies of particular interest are p63, p53 and ki67. Along with these antibodies I am looking for positive nuclear and cytoplastic staining antibodies. If anyone has any experience in staining alcohol fixed slides your information is very appreciated. All the best. -- Sincerely, - Levi Fried ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors
RE: [Histonet] Re: Staining on Alcohol Fixed Smears
Since there is no exposure to formalin, we do not do antigen retrieval on alcohol fixed specimens. We use the Ventana Benchmark XT and copy the protocol to a new number and use the Wet option rather than paraffin. No retrieval needed. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Cox Sent: Saturday, December 14, 2013 6:30 PM To: histonet@lists.utsouthwestern.edu; levi.fr...@gmail.com Subject: [Histonet] Re: Staining on Alcohol Fixed Smears Levi, I've worked up most of the more common antibodies on alcohol fixed smears. There are two things to remember here: 1) your specimens are alcohol fixed, meaning antigen retrieval needs are different, and 2) they are smears, meaning the cells weren't 'cut open' during microtomy, which impeeds some nuclear staining. Those are two different issues to consider. Some rules of thumb: 1) most cell membrane antigens will stain nicely with minimal changes to the protocols you use for FFPE specimens 2) most cytokeratin antigens stain nicely with little change to your FFPE protocols (if you need to change, start by cutting your antigen retrieval time in half) 3) most nuclear antigens require significant changes from FFPE protocols, and some are essentially impossible to stain using standard antibodies. When I return to work on Monday, I will forward you my protocol 'adjustments' for the antibodies you requested. Beth Cox, HTL/SCT(ASCP)QIHC Vagabond Histotech On 12/14/2013 12:00 PM, histonet-requ...@lists.utsouthwestern.edu wrote: Message: 4 Date: Sat, 14 Dec 2013 18:25:08 +0200 From: Levi Friedlevi.fr...@gmail.com Subject: [Histonet] Staining on Alcohol Fixed Smears. To:histonet@lists.utsouthwestern.edu Message-ID: CA+puqxTXL85pEwvR=sqPQA36tMJPm=sx2kv3gyebrbu0he3...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Hi Everyone. I am looking to stain alcohol fixed smears with a group of antibodies. The antibodies of particular interest are p63, p53 and ki67. Along with these antibodies I am looking for positive nuclear and cytoplastic staining antibodies. If anyone has any experience in staining alcohol fixed slides your information is very appreciated. All the best. -- Sincerely, - Levi Fried ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: external alarm for Tissue-Tek VIP E300
Ours is tied into the main lab system that monitors all of the refrigerators and freezers. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Juliana Montero Sent: Monday, November 18, 2013 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] external alarm for Tissue-Tek VIP E300 I am looking to get an external alarm for our tissue processor. Any suggestions? Juliana Montero, HTL Lead Histotechnologist USMD Pathology Laboratory 900 Airport FWY Ste. 132 Hurst, TX 76054 817-576-0590 Office 817-576-0591 Fax 432-238-8981 Cell juliana.mont...@usmd.commailto:juliana.mont...@usmd.com [USMD] The information contained in this e-mail message is intended exclusively for the recipient(s) named above. This communication may contain information that is confidential, proprietary or otherwise legally exempt from disclosure. If you are not the named addressee, you are not authorized to read, print, retain, copy or disseminate this message or any part of it.If you have received this message in error, please notify the sender immediately by e-mail and delete all copies of the message. Review by any individual other than the intended recipient does not waive a privileged relationship (if applicable) between the sender and the recipient. USMDhttp://www.usmdinc.com/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] FW: Fungus Controls
I made some controls years ago that we are still using today. I used bread mold and streaked it out on a blood agar plate. Once the plate was covered, cut the agar into cubes, wrapped it in lens paper and processed. Cuts and stains beautifully. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim Higgins Sent: Tuesday, November 05, 2013 2:04 PM To: histonet@lists.utsouthwestern.edu; vickroy@mhsil.com Subject: [Histonet] FW: Fungus Controls I have used orange peel for years. I never had a problem with the pathologist not approving the control for use on patient stains. Its naturally occurring, a petri dish would not be naturally occurring in my mind. Once I verified the orange peel does stain the fungus the same way it does in human tissue by doing a side by side staining the pathologist were fine with it. Actually happy I was able to save some money. Most controls you get from other source are not human either, usually it some type of animal. Try the orange peel. I did like the idea of using the some type of moldy meat or having your micro department do something for you I have never tried that, but if it works that would be awesome. Thanks, Timothy N. Higgins, HT (ASCP), QIHC Message: 4 Date: Mon, 4 Nov 2013 15:07:06 -0500 From: Patrick Laurie foreig...@gmail.com Subject: Re: [Histonet] Aspergillus tissue blocks for controls To: Vickroy, Jim vickroy@mhsil.com Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: CAKEyg-2KaDkn0oFQURzuPcugQt=ju8lkk6c8dah+npjvvsp...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Hi Jim, I have found a couple of ways. First, if you are fortunate to have a micro department nearby, they can make a very nice one for you (a Histotip in Sakura's Histologic). Another method, which doesn't work the best, can be moldy orange peel. And the easiest, you can create your own (moldy sausage) or even take some human tissue (fresh lung works the best) and leave it with some moldy meat, then you can get a great one. Good luck! On Mon, Nov 4, 2013 at 10:38 AM, Vickroy, Jim vickroy@mhsil.com wrote: We are in desperate needs of obtaining GMS controls. In the old days we had all the fungal specimens we needed. Today it is getting hard to find these tissues or controls. Does anyone have any idea where we can get tissue blocks with Aspergillus or does anyone have any other suggestions about GMS controls. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: cell block staining issues
We have not used B72.3 for quite awhile now but all of our NGYNs are either collected in or processed with Cytolyt and we have had no issues. Sorry, I know this isn't much help. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Thursday, October 31, 2013 8:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cell block staining issues We have something of a mystery here and I am hoping someone can help. My cytopath docs are saying that our B72.3 (TAG-72) antibody is not working well in cell blocks created from fluids (plural), etc. and that this is a fairly recent development (over the past 5-6 weeks or so).The surgical cases we have stained look great as does our control, which is placed on each slide. We have not changed lot numbers of antibody or made any up recently and the antibody is not expired, or even close.The only thing we can find that is different is that cytology has been rinsing the needles out with a product called Cytolyt, instead of saline and they started this around the first of August.Does anyone know if this product could cause any interference with our staining? The docs just say that the tumor cells are not staining and we are sort of at a loss here. Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Manager [Wake Forest Baptist Health] Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: freezing spray
We do not us it. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, October 24, 2013 2:45 PM To: histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] freezing spray Our hospital has not allowed freezing spray to be used in the frozen section lab for many years. We now have a new group of doctors who want to use the spray. The docs think the frozen sections take too long to freeze. Yet, they meet the frozen section TAT for more than 98% of our cases. I think it's worth not using it for the one case where no one suspects TB but the patient will be positive. Do you allow freezing spray in your frozen section lab? Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.orgmailto:hor...@archildrens.org archildrens.orghttp://www.archildrens.org/ ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Low Temp problem
We have had pads replaced as well but the problem continues. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gary Gracie Sent: Wednesday, October 23, 2013 9:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Low Temp problem I am also running Ventana Benchmark Ultras. This low temp problem has started to occur regularly to individual heater pads (five in total over several weeks, across two machines). Ventana have promptly replaced the pad on each occasion. Gary Gracie Senior Technical Officer IHC Laboratory Anatomical Pathology St Vincents Hospital Sydney, Australia Message: 1 Date: Tue, 22 Oct 2013 11:04:45 -0700 From: HERRINGTON, SHEILA sheila.herring...@interiorhealth.ca Subject: RE: [Histonet] Low Temp question To: 'Tom McNemar' tmcne...@lmhealth.org, 'Shawn Leslie ' shawnles...@embarqmail.com, Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu Message-ID: 9d5f3f245a5fe0458e46e9a71e3ee08c06cd37f...@dc1serv352.interiorhealth.ca Content-Type: text/plain; charset=utf-8 I also have been having low temp errors. I use Benchmark Ultras and they are not a batch stainer, so pads are heated independently. I noticed the staining on the particular pad with the error was not quite to the usual standard so I have stopped using that pad. Have contacted Ventana for a case number, but have not got an answer. Is this a new problem for you as well? This only started a few weeks ago for us. Sheila Herrington Technical Lead Histopathology and Immunohistochemistry Kelowna General Hospital 2268 Pandosy Street, Kelowna, B.C. V1Y 1T2 250-862-4300 ext 7587 or 7510 sheila.herring...@interiorhealth.ca -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Tuesday, October 22, 2013 8:28 AM To: 'Shawn Leslie '; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Low Temp question We see these continually as well. We are told that it is a software glitch and not really a problem with the heat. I was told that they are working on it. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.orghttp://www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shawn Leslie Sent: Tuesday, October 22, 2013 11:07 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Low Temp question Goodmorning all, I have a question concerning the Ventana Benchmark XT. We are constantly having low temperature errors during almost every run. The immunos look fine but we are still concerned. Has anyone else that uses the Benchmark experienced this also ? We have noticed that the silver coating on the heating pads is wearing off. Ventana indicated to us that it's nothing to be concerned about. Any hel p would be appreciated. Shawn Leslie HT (ASCP) ** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager. This footnote also confirms that this email message has been virus scanned and although no viruses were detected by the system, St Vincents Mater Health Sydney accepts no liability for any consequential damage resulting from email containing any computer viruses. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents
RE: [Histonet] Low Temp question
We see these continually as well. We are told that it is a software glitch and not really a problem with the heat. I was told that they are working on it. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shawn Leslie Sent: Tuesday, October 22, 2013 11:07 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Low Temp question Goodmorning all, I have a question concerning the Ventana Benchmark XT. We are constantly having low temperature errors during almost every run. The immunos look fine but we are still concerned. Has anyone else that uses the Benchmark experienced this also ? We have noticed that the silver coating on the heating pads is wearing off. Ventana indicated to us that it's nothing to be concerned about. Any hel p would be appreciated. Shawn Leslie HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Dress codes
Hospital dress code. Scrub uniforms purchased through the hospital. No open toed shoes. None-uniformed employees are casual/business attire. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wineman, Terra Sent: Tuesday, October 08, 2013 1:39 PM To: Wineman, Terra; Horn, Hazel V; 'Nancy Schmitt'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Dress codes Regulatory agencies - OSHA, NIH, CDC for sure!! Terra Wineman, HTL (ASCP)CM Research Biologist 636-926-7476 phone terra.wine...@novusint.com -Original Message- From: Wineman, Terra Sent: Tuesday, October 08, 2013 12:35 PM To: 'Horn, Hazel V'; 'Nancy Schmitt'; histonet@lists.utsouthwestern.edu Subject: RE: Dress codes In the research lab the dress code is an follows, close toed shoes this means no crocs or dress shoes with long pants, no shorts or dresses. Safety glasses per OSHA and nothing loose that might get caught in machinery. Terra Wineman, HTL (ASCP)CM Research Biologist 636-926-7476 phone terra.wine...@novusint.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Tuesday, October 08, 2013 12:23 PM To: 'Nancy Schmitt'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Dress codes We have a hospital dress code. It is not just for the lab. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.org archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Tuesday, October 08, 2013 12:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Dress codes Not sure if my mail is going through or not... Thank you Nancy Schmitt Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories 205 Bluff Street Dubuque, IA 52001 563-556-2010 ext. 142 nancy_schm...@pa-ucl.com From: Nancy Schmitt Sent: Tuesday, October 08, 2013 8:58 AM To: 'histonet@lists.utsouthwestern.edu' Subject: Dress codes Good Morning- I am looking for input on dress codes - specifically shoe and pants guidelines and what regulatory agency your information comes from. It seems this can be a hot button topic:( Thank you for your input- Nancy Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories nancy_schm...@pa-ucl.commailto:nancy_schm...@pa-ucl.com NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from
[Histonet] IHC trun-around-time
Hello all, I am wondering how many places offer same-day staining of IHC. We are a small hospital based lab that averages about 70 IHC slides per week. We operate 5 days/week, 0600-1600. We give same day staining for IHC requests that are received by 10am and are using the Ventana Benchmark XT. Due to some issues that have developed, we may have to change the way we do things. With our current 10am cutoff plus possible pre-analytical changes the instrument will finish too late in the afternoon. The pathologists really like the same day service on IHC but I'm thinking that I may have to make the cutoff time earlier and anything after that will just have to wait. So I am wondering if anyone else of similar size, instrumentation, and workload, offers same-day staining for IHC. I appreciate your input. Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cell Block Preparation
This is how we do it now. In the old days, we used agar and to my mind, it is still the best way when you have scant material. - Spin in a conical tube and pour off - Melt an agar slant (we get TSA slant from micro) - Pour the agar into the conical tube and spin for 5 minutes - The agar will re-solidify and whatever sediment there is will be concentrated in the very tip of the cone - The agar will slide out of the centrifuge tube - Slice off the very tip and wrap in lens paper - Place the wrapped tip in a cassette and process as usual - Embed the specimen tip down and you are good to go... I still use this method today when I feel it necessary. Works great. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Specian Sent: Thursday, September 05, 2013 12:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cell Block Preparation I am getting complaints in regard to insufficient cell blocks. We currently spin, pour off the supernatant, retrieve the sediment and process in lens paper. Does anyone have a more current technique which renders better cellularity? Also, do you know which renders a better cell block: a fresh specimen, a specimen fixed in Cytolyt or a specimen fixed in 10% NBF? Thanks, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Unstained slides
We cut a decent number of unstained as well. Mostly the tiny biopsies and cores and we do end up using most of these. I personally see no reason to cut unstained slides on anything else. Since you still have to go back and cut controls, it doesn't really save that much time but of course, it does save tissue. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Brinegar Sent: Wednesday, August 28, 2013 4:47 PM To: Martha Ward-Pathology Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Unstained slides Martha, I have had experience in three different labs. The unstained slides can pile up quickly, especially when you are using charged slides that are just in case slides for sendouts, IHC, etc. For core biopsies (breasts, fine needles, prostate, etc.) we cut levels and put up at least five of the in between sections to keep on hand in case. We maybe use these slides 10 percent of the time and toss the rest after 3-4 weeks. It can seem wasteful, however if you have a small core, you must save precious tissue. Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 On Wed, Aug 28, 2013 at 9:28 AM, Martha Ward-Pathology mw...@wakehealth.edu wrote: We are looking at ways to improve our work processes, save time and labor and reduce costs, all while maintaining patient quality...as we all are of course. During our conversations the subject of cutting unstained slides has come up and we are looking for bench marking data to see if we are where we need to be.Currently we are cutting unstained slides for various protocols (including prostate biopsies), where the specimens are tiny, but we are also cutting a lot of just in case unstained slides. Our research has shown that about 50% of the time the unstained slides requested are never used and we are trying to find out if that is high, low or about what other institutions are seeing. If anyone has any data they could share with us we would appreciate it. Thanks in advance for your help. I can share what I find out if others are interested. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Two Patient Identifiers on slides
Yes. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: Pratt, Caroline [mailto:caroline.pr...@uphs.upenn.edu] Sent: Wednesday, July 31, 2013 1:47 PM To: Tom McNemar; Jean Wood; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Two Patient Identifiers on slides Just for clarification, patient first and last name? Thanks! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Wednesday, July 31, 2013 5:49 AM To: 'Jean Wood'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Two Patient Identifiers on slides Our cassettes are printed with the accession number, patient name, and a barcode that also contains the DOB. When sectioning, this barcode is scanned at the microtome when the slides are cut. Labels are printed, again at the microtome, and the slides are labeled. The slide label contains the accession number, patient name, level number or special stain, the pathologist's initials, and the hospital address. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jean Wood Sent: Tuesday, July 30, 2013 3:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Two Patient Identifiers on slides Hello Histonetters, Recently we started utilizing a slide labeling component that is built into our AP Easy LIS system and has accession number, levels and patients first and last name when labels are printed out. Dymo does not have a chemically resistant label (we have a Dymo 450 printer) and we have been putting the labels on AFTER the slides are stained and cover slipped. In the meantime, the HT is writing in pencil the accession # and levels on the slide which is then covered up with the permanent label after cover slipping. Our Lab Manager is worried that we are not compliant as we do not have two patient identifiers on throughout the whole process (she wants us to write patient names on slides in pencil (before staining) and then cover that up with the pre-printed label after staining. 1. What is everyone else doing? 2. Have any of you found a chemically resistant label compatible with the Dymo labeler? Jean Wood BS, HT Fairchild Medical Center Pathology Dept. Ph:530.841.6243 Fax:530.841.6232 jw...@fairchildmed.orgmailto:jw...@fairchildmed.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you
RE: [Histonet] Two Patient Identifiers on slides
Our blocks have a barcode to the left then two lines of text. The top line is the accession number in a large font the name below it. The name is printed in a smaller font but it is easily readable. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pratt, Caroline Sent: Wednesday, July 31, 2013 2:14 PM To: Joe W. Walker, Jr.; Michelle Moore; Jean Wood; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Two Patient Identifiers on slides My understanding was the same as Joe's and at least to date, that is what we have presented during survey and it has been deemed acceptable. However, we are accredited by TJC not CAP. My question to those of you who are providing 2 patient identifiers on blocks? How do you make it fit? If anyone could scan an image to caroline.pr...@uphs.upenn.edu I would greatly appreciate it! As of now, we have the unique patient accession number and a barcode with the accession number as well. We are in our survey window I would like to be sure we are compliant. One more question, if you label slides and blocks with a name and DOB from the requisition and find out during insurance validation that the name is misspelled or the DOB is inaccurate, how do you handle that? Thanks for your help. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe W. Walker, Jr. Sent: Tuesday, July 30, 2013 4:31 PM To: Michelle Moore; Jean Wood; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Two Patient Identifiers on slides I don't think this is technically correct according to the CAP. The question in the most recent checklist states: **REVISED** 07/11/2011 ANP.52000 Specimen ID Phase II The identity of every specimen and image, including blocks, slides, and electron micrographs, is maintained through each step in processing. NOTE: Each block of tissue must be individually labeled with unique patient identifier(s), e.g. accession number etched onto the block or embedded into it. Storage of unlabeled blocks in separate containers that are labeled with patient number or name does not meet this requirement. Evidence of Compliance: ✓ Written procedure describing system for maintaining specimen identity This question does not state that 2 identifiers must be on the slides and blocks, etc. It states it must be a unique identifier throughout the process. A specimen accession number would technically suffice. You can add additional identifiers if that helps in your process but they are not required. I believe that this is often confused with the General lab question GEN.40491 which requires that any specimen that is collected and submitted to the lab must have 2 unique identifiers on it. Once the specimen is in the lab, the unique accession number would suffice. Now, if you are utilizing the 2 identifiers to help prevent mislabeling, then I think it is a good idea as an extra precaution step. We are fortunate to have a xylene resistant label and printer that allows us to label the slides prior to processing. My two cents as a manager, Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewal...@rrmc.orgwww.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional…Vermont’s 1st Hospital to Achieve Both ANCC Magnet Recognition® and the Governor’s Award for Performance Excellence -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michelle Moore Sent: Tuesday, July 30, 2013 3:46 PM To: Jean Wood; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Two Patient Identifiers on slides We are writing path #, date of birth patient initials as identifiers. Michelle From: Jean Wood jw...@fairchildmed.org To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Tuesday, July 30, 2013 2:37 PM Subject: [Histonet] Two Patient Identifiers on slides Hello Histonetters, Recently we started utilizing a slide labeling component that is built into our AP Easy LIS system and has accession number, levels and patients first and last name when labels are printed out. Dymo does not have a chemically resistant label (we have a Dymo 450 printer) and we have been putting the labels on AFTER the slides are stained and cover slipped. In the meantime, the HT is writing in pencil the accession # and levels on the slide which is then covered up with the permanent label after cover slipping. Our Lab
[Histonet] RE: Two Patient Identifiers on slides
Our cassettes are printed with the accession number, patient name, and a barcode that also contains the DOB. When sectioning, this barcode is scanned at the microtome when the slides are cut. Labels are printed, again at the microtome, and the slides are labeled. The slide label contains the accession number, patient name, level number or special stain, the pathologist's initials, and the hospital address. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jean Wood Sent: Tuesday, July 30, 2013 3:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Two Patient Identifiers on slides Hello Histonetters, Recently we started utilizing a slide labeling component that is built into our AP Easy LIS system and has accession number, levels and patients first and last name when labels are printed out. Dymo does not have a chemically resistant label (we have a Dymo 450 printer) and we have been putting the labels on AFTER the slides are stained and cover slipped. In the meantime, the HT is writing in pencil the accession # and levels on the slide which is then covered up with the permanent label after cover slipping. Our Lab Manager is worried that we are not compliant as we do not have two patient identifiers on throughout the whole process (she wants us to write patient names on slides in pencil (before staining) and then cover that up with the pre-printed label after staining. 1. What is everyone else doing? 2. Have any of you found a chemically resistant label compatible with the Dymo labeler? Jean Wood BS, HT Fairchild Medical Center Pathology Dept. Ph:530.841.6243 Fax:530.841.6232 jw...@fairchildmed.orgmailto:jw...@fairchildmed.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microtome advise...
Hello all. I am preparing my budget and am seeking recommendations on automated microtomes. I am specifically interested in brands other than Leica (I already have two). I would appreciate any thoughts you may have. Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Label identifiers
Whoops! I guess you probably weren’t asking about slide labels. For container labels, it is barcode, patient name, account number, specimen source, and container number. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Friday, July 12, 2013 11:14 AM To: 'Amber McKenzie'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Label identifiers We have patient name, accession number, the pathologist's initials, hospital name and address, and special stain, or HE, or level, etc. The accession number and DOB is also included in a barcode that is printed on the label. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Friday, July 12, 2013 9:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Label identifiers I'm trying to get our Co-path computer system set up to print labels instead of us handwriting on the paperwork and containers...what information do you guys have on your printed labels...just patient name and accession no? Thanks! This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] bubbles in paraffin blocks
I see this when using the cassettes with the smaller holes and when the paraffin is added too fast. Air gets trapped under the cassette. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of j...@imebinc.com Sent: Wednesday, July 10, 2013 12:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] bubbles in paraffin blocks Histonet experts,can you give me reason why embedded paraffin would have tiny bubbles in the block after the cooling process? Any idea are appreciated John O'Brien IMEB, INC The Pathology Specialists Tel 800-543-8496 Fax:760-761-0859 j...@imebinc.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: p16 control tissue
We are using Biocare's ab with squamous cell ca as the control of choice. I believe you can also use cervical dysplasia or tonsil. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Tuesday, July 09, 2013 1:50 PM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] p16 control tissue We are going to be running the p16 antibody from Biocare (RUO) on skin specimens. What type of tissue should I run for a positive control? The data sheet says normal testis.. Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Disposable blade information
I didn't think anybody still used steel knives I remember those days I would say to get away from the steel as soon as you can. Disposable will make your life so much easier. As you already know, it is near impossible to give you anything more than a guestimate but I would imagine that you could get by with one to three per day depending on case mix, staples, sutures, calcifications, etc. There are so many variables. It also depends on how many people are cutting those 50 blocks. There are a lot blades to choose from. You could probably get by for quite awhile just trying out different samples See if you can find a rep that can loan you a blade holder and try some. The holder will be the biggest upfront expense. I don't really know what the difference is between the high and low profiles but I suspect not much more than the height. I have tried many different brands over the years and always go back to the AccuEdge low profile. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Friday, June 28, 2013 12:03 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Disposable blade information Good Morning all, I am having to do a cost analysis study for the cost effectiveness of Sharpening steel blades vs. transitioning to disposable blades. I know...what you all are thinking.. Your're still using steel blades??...Yes, we are... :). Our current knife sharpener has some issues, and I have decided to weigh buying a used knife sharpener against switching to the disposable system. So... a little background for you. We are a relatively small department, we average @ 50 blocks a day, of various tissues types. My questions to you are this...and I know that answers will only be estimations...but it is a place for me to start. What would be the average number of disposable blades that I would probably use in a day/ week? On an average...how many blocks can be cut on each blade? Has anyone recently made this transitiion? If so, what were some of the challenges?? I'm going to show my lack of knowledge of disposable blades with this next question...but here it goes.. What is the difference between a low profile blade and a high profile blade...and would I need to buy both?? Ok... my head is hurting...so.. I am going to leave you with all the above questions. Any and all information, suggestions, etc. will be greatly appreciated!! Thanks and have a GREAT day!! Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.han...@parrishmed.com = This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you = ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Cytology Staining
We stain all fluids separately and check for malignancy. If malignant, the stains are changed before staining anything else. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McCabe, Sara Sent: Wednesday, June 26, 2013 3:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cytology Staining I have a question for those of you that also may perform cytology specimens at your facility. How do you prevent carryover from one case to another? For example, we had obvious carryover from a pleural fluid onto an FNA of a thyroid case. This has never happened here (to our knowledge). Has anyone else every experienced this? We spray fixed our slides with an alcohol/acetone based fixative before staining them. Any advice would be appreciated! Sara McCabe, HT(ASCP) DuBois Regional Medical Center This email and any attached files are confidential and intended solely for the intended recipient(s). If you are not the named recipient you should not read, distribute, copy or alter this email. Any views or opinions expressed in this email are those of the author and do not represent those of the company. Warning: Although precautions have been taken to make sure no viruses are present in this email, the company cannot accept responsibility for any loss or damage that arise from the use of this email or attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Block labeling
I used to use a 4x4 with a little Americlear or Xylene to clean the paraffin off before relabeling. You can then dip or run a little paraffin over it. May not be practical if you have a lot. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie Sent: Monday, June 24, 2013 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block labeling Hi Everyone, What is the best way to label blocks that are already embedded? We have a large control bank that we are trying to relabel and make search able, however, most marking pens don't write well on paraffin covered blocks, even when we scrape them off. Pencils work, not always the most legibly, so I was wondering if there is a clever solution that someone might have found. We've tried putting stickers or labels on the back, but that falls right off. Thanks in advance for any suggestions. -- Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Billing ???
Hospital bills the technical and pathologists bill the professional. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jmasla...@stpetes.org Sent: Friday, June 21, 2013 11:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Billing ??? Happy Friday This is a question to those who work at a hospital that have contracted pathologists. Does your hospital bill the technical component and the pathologist bill the profession component or does the pathologist bill everything and pay the hospital for technical component? Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor Give thanks for ALL things. Kindness is the language the blind can see the deaf can hear- Mark Twain This electronic mail message contains information which is confidential. If you are not the intended recipient, please be aware that any disclosure, photocopying, distribution or use of the contents of the received information is prohibited. If you have received this e-mail in error, please reply to the sender immediately and permanently delete this message and all copies of it. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Xylene substitutes for tissue processor clean cycle
We use Americlear. Have not used Xylene for many, many years. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Conway, Carla Sent: Tuesday, May 21, 2013 11:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene substitutes for tissue processor clean cycle Hello all, The technician who does preventive maintenance on our LX-120 tissue processor recommended that we use xylene as the solvent in our clean cycle. We are considering using a xylene substitue in the clean cycle and would appreciate any recommendations. Thanks very much, Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-6282 ext. 242 Fax: 206-526-6654 E-mail: cmcon...@usgs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Lysol IC for Ventana equipmetn cleaner
We use it but we only make it up 1 gallon at a time. We use 20ml to 1 gallon of distilled per Ventana. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, May 14, 2013 12:33 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Lysol IC for Ventana equipmetn cleaner I used to use Amphyl to decon my Ventana equipment, but now Cardinal sells Lysol IC in its place and I was wondering how you guys make it up...water to cleaner ratio in the Vantana carboys. With Amphyl I did 20L dH20/200ml Amphyl. Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: CAP standard GEN 61300
I am very interested in this as well. We have an ongoing issue with humidity/static. I have been unable to find anything that lists a recommended humidity level for histology. New construction building codes for labs list 30% - 80% and the equipment manufacturers list 30% - 80% for their recommended operating environment. Based on two different digital readers, our humidity is sometimes in the 20% range (mostly in winter but not always). A hand operated device reads about 40% higher than the digitals so I don't quite know what to make of it. Anyway, I will appreciated any input. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Knutson, Deanne Sent: Monday, May 06, 2013 8:13 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] CAP standard GEN 61300 Fellow Histonetters, I am looking for advice concerning CAP standard GEN 61300 Climate Control. To those that take humidity records in your lab, what exact % range do you use for a normal range? And what do you do if and when you are out of this range? Thank you for sharing your workflow info with me. Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 701-530-6730 dknut...@primecare.orgmailto:dknut...@primecare.org This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: IHC (DAB) after HE?
We just stain right over the HE. No problems. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Thursday, April 04, 2013 5:24 PM To: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu; histonet-requ...@lists.utsouthwestern.edu Subject: [Histonet] IHC (DAB) after HE? Dear Histonetters, I would like to perform IHC (DAB) after HE. Do I need to remove HE? If answer is YES, my question HOW? Can I ask the protocol for that? Thanks in advance, Naira ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Cell block preparation
We wrap them in lens paper. No problems. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J Sent: Friday, March 29, 2013 12:41 PM To: Histonet Subject: [Histonet] Cell block preparation Hello Histonet, What is everyone doing regarding cell block preparation? We have been using sponges for processing but we've had a recent contamination issue that may be attributable to the sponges (fluid types vary but mostly pleural fluids). We will be testing sponges, biopsy bags, and paper... anyone have a method they prefer? Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Tracking OR specimens
Our OR uses a binder with log sheets. They write the specimen source one of the left over patient labels and place it onto the log sheet. We then match them up with the actual specimens received and initial the label. It works very well since any discrepancies are cleared up before the specimens ever leave the OR. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michelle Lamphere Sent: Monday, March 18, 2013 9:14 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Tracking OR specimens Are there any histology labs that actively participate in auditing the Operating Room on a daily basis to make sure that histology receives all of the specimens that the OR should have submitted? If so, how do you do this? Or should the OR be solely responsible for making sure that they specimens make it to histology? Michelle M Lamphere, HT (ASCP) Senior Tech, Histology Children's Medical Center 1935 Medical District Drive Dallas, TX 75235 Office :214-456-2798 Histology: 214-456-2318 Fax: 214-456-0779 Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456- or via e-mail at priv...@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456- or via e-mail at priv...@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: General Data
We use a full PC mounted under the counter and a touchpad mounted on an arm that can be positioned just about anyplace. The printer is mounted on a small shelf below the counter. Small scanner mounted to a desktop stand that can be moved wherever the cutter wants. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Thursday, February 14, 2013 11:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] General Data Good Morning- We are currently using General Data for cassette labeling - very happy with it. The next phase is implementing slide labeling. I would appreciate knowing what kind of setup people are using at the microtome - laptop? touchpad? Full computer? Is the arm off of the microtome or out from the counter? Any and all information is appreciated! Nancy Schmitt, HT, MLT(ASCP) Histology Coordinator Dubuque, IA 52001 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: grossing tools
We have a pair of the 3mm Cutmate forceps that we bought years ago for a particular pathologist but nobody uses them. If you go to the website below check out the Procut as well. http://www.milestonemed.com/histopathology/products.html Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Wednesday, February 13, 2013 9:57 AM To: Bob Richmond; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: grossing tools That is why I suggested having a rep. bring them out to demonstrate. Sakura sells these. The grossing boards are items: 4800, 4801 and 4802. The grossing forks are 4803, 4804 and 4807. Ask your rep for a demo. Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, February 13, 2013 9:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: grossing tools Jeanine H. Bartlett at the CDC notes: Sakura sells these. The grossing boards are items: 4800, 4801 and 4802. The grossing forks are 4803, 4804 and 4807. Ask your rep for a demo. http://sakura.ifuture.com/sakura.asp?ifut=1000336642970309899%2CTKWK%3E6%3D%3A5ST0 The prices of these tools are outrageous. The online catalog gives me no clue as to how they work. Bob Richmond Samurai Pathologist Maryville TN On Tue, Feb 12, 2013 at 8:01 PM, Bob Richmond rsrichm...@gmail.com wrote: Bruce Gapinsk HT (ASCP), Chief Histologist, Marin Medical Laboratories, PathGroup SF asks: Histonians, I'm sure we are not the only histology lab that deals with thick grossed specimens. Has anyone tried the new gross tools by Sakura? Or anything else that can cut ONE nickel thick. Tired of reprocessing. This grumpy old (74) pathologist would be happy to try them out, though I can cut freehand and almost never have a block reprocessed, but the skill eludes a lot of the young folks. Do you have a Web link for these tools? The problem would be getting a lab manager to spring for them. The Laboratory Manager's Handy-Dandy Guide to Making Life Hard for the Pathologist (I'm sure there is such a book, though I haven't actually seen one) specifies that grossing is a ritual requiring only a basic set of tools. One lab expected me to gross with an athame and chalice. I tried to explain to them that OSHA and the CAP would not permit me to gross skyclad. (You guys know I NEVER exaggerate.) Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Process and hold?
Hello all, I was wondering about processing breast specimens (needle cores) on Fridays. We have asked our radiology department to try to avoid scheduling these breast biopsies on Fridays since we do not work weekends and are concerned about the extended time in formalin. I am thinking that we can run these specimens on a second processor over Friday night and have someone from the clinical lab come up and drain the paraffin. The tissues would then sit in a warm moist retort until Monday morning when they would be embedded and cut. I think the specimens would be fine. Processing would be complete at that point and they would hold in the unopened retort chamber. Our alternative is to have someone come in every Saturday morning just to remove and embed these specimens. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Temperatures
We are JCAHO and this has never come up but that doesn't mean that it won't. I don’t know about your specific processor but ours (VIP5), will fail to start and alarm if the paraffin temp is out of range. Our processor is tied in to the main lab temperature monitoring system so we get a call anytime there is an alarm. Such a system is probably not feasible for a processor alone but does your main lab have anything in place? Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sullivan, Beatrice Sent: Friday, February 08, 2013 10:32 AM To: Rene J Buesa; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Temperatures Because I have been told that according to JCAHO (who will be inspecting us) that if a temperature is required to ascertain consistency, quality and integrity, you will have to record temperature 7 days per week. From: Rene J Buesa [mailto:rjbu...@yahoo.com] Sent: Friday, February 08, 2013 10:17 AM To: Sullivan, Beatrice; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Temperatures And my question is: why are you bothered by not knowing the temp. in a processor that only starts to process on Sundays? If the processor is emptied on Mondays to embed→cut, take the temp. then. Other that this you would have to place a sensor and a recording device or you would have to make somebody to go to the lab. For just write down the melted paraffin temp.? Too much expense and trouble for an non-existent problem. René J. From: Sullivan, Beatrice bsulli...@virtua.orgmailto:bsulli...@virtua.org To: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu Sent: Friday, February 8, 2013 7:52 AM Subject: [Histonet] Temperatures I'm looking for a fix to our problem of no temperatures being taken on the weekends. We are closed and this is creating an issue. Our processors are not running until Sunday night but the paraffin in both the processors and embedding center are kept molten. Any help would be greatly appreciated Beatrice L. Sullivan HT(ASCP)HTL Corporate Histology Manager Virtua, Voorhees 856-247-3144 This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to issecur...@virtua.orgmailto:issecur...@virtua.org. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to issecur...@virtua.org. Thank you This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you
[Histonet] Processor dehydration cycles..
Hello all, I was wondering what most people use as the first reagent after the formalins on their tissue processor? We have always used a sequence of 70%, 80%, 95%, and 100% but is anyone using 80% or even 95% to start their dehydration? Thanks in advance. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Xylene Substitutes
Not familiar with SubX but we have used Americlear for many years with good results. You just can't beat Xylene for some things though and still keep a little around. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson Sent: Tuesday, February 05, 2013 4:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Substitutes Hello all, My lab is looking into xylene substitutes, and I'd love some feedback on what other labs are using. We currently use SubX, but are there other items out there more economical? Thanks, Adrienne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Pathologists' Names on HE Labels
We have recently moved to printed/barcoded labels for all of our slides. Each pathologist has a mnemonic in our LIS (Meditech) that we use when assigning the case. This mnemonic is picked up and printed on the slide label. It has been a real help, especially on recuts and stains. We no longer have to look up a specimen to see who it goes to. The mnemonic is just the pathologist's initials and I can see no reason why this should be a problem. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roger Heyna Sent: Friday, January 04, 2013 5:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathologists' Names on HE Labels For years, our lab's information system has been set up so that the name of the pathologist assigned to a case prints on our HE labels to assist with sorting and grouping the slides after staining. As new pathologists join our team, concerns regarding this practice have come up. The pathologists are concerned that having their names on the slides pose medical-legal liability, and they want the names removed. I've yet to hear clear ramifications involved with having doctors' names on slides. What liability is really involved? We are a large, urban academic medical center with high specimen volumes. Our pathologists rotate through different sub-specialties, sometimes on a daily basis. For us, having the pathologists' names on the HE labels seems to be the easiest way to get the HE's to the correct pathologist. Are other labs printing the names of the pathologists on their HE labels? Is anyone aware of any legal risk involved in this practice? Would anyone mind sharing how they sort and divide the slides before submitting them to their pathologists? Thank you, Roger Heyna, BS, HTL(ASCP) Loyola University Medical Center Maywood, IL This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] 2 CAP questions
All of our equipment is tied into the hospital emergency power system. Our tissue processor is also on a separate UPS backup just in case. Since we are a dayshift operation only and separated from the main lab, the processor is also monitored by the laboratory alarm system in the event of an overnight error condition. If there is a problem, I get a call. All laboratory thermometers (and timers) are calibrated yearly by an outside company that also covers pms and repair work. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of pathr...@comcast.net Sent: Wednesday, December 19, 2012 12:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] 2 CAP questions Fellow techs, 1) what type of a back up power source are you using for your tissue processors, refridgerators and freezors? 2) What do you do about calibration for NIST thermometers and non certified thermometers? Thanks, Ron ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Trichrome
Same problem here. Using the Nexus stainer and have also experienced this with the Reticulin as well. Called service today and they elected to send an application specialist instead of a service tech. Will see what happens. Would not have expected to see this when doing the stain by hand Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Tuesday, December 04, 2012 8:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Trichrome Hi- need help troubleshooting a trichrome. The stain on the Nexus stainers is inconsistent. Taking it off the stainer and doing it by hand with a kit has similar results. The tissue is NBF fixed needle biopsies on kidney. There is a little mechanical damage on the edges of the tissue but the collagens are staining too light and the red overstains. The normal tissue as control is beautiful -- I know I know--don't force the stain--but it doesn't make sense to have this much variation from a kit or auto stainer... Help? Cheryl Kerry, HT(ASCP) Path Group of Louisiana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Trichrome
Forgot to mention that we are also getting areas of zero staining as well. Needle biopsies are beautiful on one end and totally stain free on the other end. Hit and miss... no consistency what so ever. Ran 2 identical slides for MT on opposite sides of the carousel... one small piece of the specimen was beautifully stained on one slide and so overstained on the other as to be unreadable. One had cores that stained from one end to the other and the other slide had cores stained only half way. Routine maintenance has been performed regularly. Seems like an instrument problem to me Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Tuesday, December 04, 2012 8:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Trichrome Hi- need help troubleshooting a trichrome. The stain on the Nexus stainers is inconsistent. Taking it off the stainer and doing it by hand with a kit has similar results. The tissue is NBF fixed needle biopsies on kidney. There is a little mechanical damage on the edges of the tissue but the collagens are staining too light and the red overstains. The normal tissue as control is beautiful -- I know I know--don't force the stain--but it doesn't make sense to have this much variation from a kit or auto stainer... Help? Cheryl Kerry, HT(ASCP) Path Group of Louisiana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: staffing numbers
Allison, We are right around 7k cases/year, 20k blocks, and about 33k slides. We have 3 tech including myself as cutter and embedder. The other two tech also cover grossing, special stains, IHC, and cytology prep. We have 1 lab assistant who also helps out with grossing (doesn't do frozen) and cytology. We are also looking to hire another assistant to do filing, etc. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hutton, Allison Sent: Tuesday, November 13, 2012 11:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] staffing numbers Hi Everyone, I am looking for some crude numbers regarding staffing. I would like to know the number of cases done per year and your number of histotechs. Any information will be appreciated Thank you Allison ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Stain for HP
IHC on the Benchmark. We routinely do them on all stomach biopsies. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Wednesday, October 17, 2012 11:04 AM To: Histonet Subject: [Histonet] Stain for HP What stain are you using for HP?--Giemsa. Warthin Starry or IHC. Do you do them routinely or only when requested? Are they done on an autostainer or with a kit? Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tossing old tissues...
Hello all, A question came up during our last JCAHO inspection regarding the dumping of old tissues. Do you just throw out the intact specimen containers with formalin and all into biohazard trash? Do you drain/collect the formalin for other disposal and just place the drained tissues into the biohazard trash? If you drain/collect, do you wear a respirator while doing it? We have always drained and only thrown the tissues into the bio trash and have not used a respirator. We monitor regularly and always make certain to include this task (along with the making of formalin) when monitoring for exposure and are always way below the acceptable limits. I don't think we have ever done and STEL just for the dumping of tissues though. Just wondered what everyone else did. I would like to stop draining and just dispose of container and all but we pay by the pound for disposal. Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Remote processor alarms
We are tied into the main lab system. It is a wireless system from Rees Scientific. We have the VIP 5 and it monitors the voltage. I get a call if there is an error of any type. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of angela smith Sent: Monday, August 27, 2012 4:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Remote processor alarms What kind of remote alarms do you all have on your various processors when something goes wrong when you are not open but have processors running. So far I found sensaphone for the Peloris. I am interested in all types. Thank you Angela Smith ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Two identifiers...
I am a little confused. Are you referring to specimen bottles? I was inquiring about what identifiers are being used on printed cassettes. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: Pratt, Caroline [mailto:caroline.pr...@uphs.upenn.edu] Sent: Thursday, August 16, 2012 9:47 AM To: Ron; pamar...@uams.edu; Tom McNemar; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Two identifiers... It doesn't meet criteria for TJC, we used that for years and it was acceptable until our last survey. We were written up for it and had to send labeling instructions to all of our submitting clinicians and conduct bottle audits to evidence that 90% of all bottles had patient full name (last and first), DOB and site along with the accession number. It is a tremendous ongoing effort, but we are there now. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ron Sent: Wednesday, August 15, 2012 12:39 PM To: pamar...@uams.edu; tmcne...@lmhealth.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Two identifiers... We do the same as Pam with the patient name and case number Sent from my Verizon Wireless 4G LTE smartphone Marcum, Pamela A pamar...@uams.edu wrote: We use the specimen or accessioning number and the patient name as the two identifiers and that seems to meet the criteria. We use the Thermo cassette writer and it transfers the information to the slides. Pam Marcum UAMS -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Wednesday, August 15, 2012 10:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Two identifiers... Hello all, For those of you using cassette and slide labelers Does your system permit you to print the accession number, patient name, and DOB on the face of the cassette? Are you using the specimen number and name as your two identifiers? Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Applica tion%20Data\Microsoft\Signatures\www.LMHealth.org This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended
[Histonet] Two identifiers...
Hello all, For those of you using cassette and slide labelers Does your system permit you to print the accession number, patient name, and DOB on the face of the cassette? Are you using the specimen number and name as your two identifiers? Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] tissue highlighting for visibility
We just add about 10-15 ml of eosin in the last alcohol on the processor Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of cont...@histocare.com Sent: Tuesday, August 07, 2012 6:10 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] tissue highlighting for visibility Hello all, Earlier today I had a VERY tiny sample from the esophogus. When I say it was tiny, it looked to be only a few microns in thickness. It was inside of, you guessed it, a teabag! :) But that wasn't the problem, as it was appropriate in this case to be put in a teabag because of the size. When I pulled it out of the cassette, I had to go over it very carefully to even find it. It's sad that I know of a not insignificant number of people who wouldn't have taken the time to find it and most likely have dispositioned it as not surviving processing or no tissue found, but that is another issue. I'm sure the patient would appreciate the extra effort. I know of a few techniques to make tissue, and specifically tiny or fatty tissue, more easily visible in cases like these. For example, I've seen using a different colored wax or putting eosin in the alcohol during processing. What do some of you guys do? www.HistoCare.com Histology Staffing ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Hologic Imaging System
Hello all. For anyone who is using the Hologic Imaging System for Cytology I was wondering about the pre-analytical steps involved. I would appreciate any thoughts you could share regarding the process of preparation and scanning. Equipment, space requirements, time, labeling/barcoding, etc. Thanks so much! Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: processors
VIP all the way. Still got one that I bought in 1985 as a backup Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Tuesday, June 12, 2012 11:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] processors Hi Histonetters- Is anybody using the ASP6025 from Leica? Pros and cons please. Then I would also appreciate any input on the Leica ASP300 vs Sakura VIP6. As always, Thank You for your help - it is highly appreciated. Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories 205 Bluff Street Dubuque, IA 52001 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Approval and review of procedures
As supervisor of Histology, I review and sign off on the SOPs yearly. Any new or altered are final approved by our medical director. The medical director also signs off on the hardcopy manuals each year. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sproles, Judy A. Sent: Wednesday, June 06, 2012 5:30 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Approval and review of procedures Hello, I am currently helping organize histology SOPs and need some help clarifying who can perform SOP review. In cytology, the technical supervisor (MD) reviews and approves procedures. CLIA rules for clinical departments allow MT as technical supervisors. Can an HT be a technical supervisor and perform annual review of SOPs? Judy Sproles CT(ASCP Methodist. Leading Medicine. Recognized by U.S.News World Report as one of America's Best Hospitals in 13 specialties. Named to FORTUNE® Magazine's 100 Best Companies to Work For® list seven years in a row. Designated as a Magnet hospital for excellence in nursing. Visit us at methodisthealth.com. Follow us at twitter.com/MethodistHosp and www.facebook.com/MethodistHospital. ***CONFIDENTIALITY NOTICE*** This e-mail is the property of The Methodist Hospital and/or its relevant affiliates and may contain restricted and privileged material for the sole use of the intended recipient(s). Any review, use, distribution or disclosure by others is strictly prohibited. If you are not the intended recipient (or authorized to receive for the recipient), please contact the sender and delete all copies of the message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Color Blindness Testing
I've been watching this thread with great interest I have been in Histology for 30+ years now and am myself colorblind (at least according to the Unites States Navy). I do see color even though I could not pass the old card test (failed it 3 times). My problem is with shades of color. I cannot distinguish real light shades or real dark shades. I started my laboratory life about 32 years ago on an aircraft carrier and was trained to read diffs, ph strips, etc. by a senior lab tech who was... you guessed it, color blind! The point of all of this is that you can do histology even if you cannot pass the test for colorblindness. For myself, if I know what a color is supposed to be and what that color looks like to me, I generally do not have any problems at all. As mentioned below, I too have trouble with acid fast stains. I cannot judge the stain quality of the acid fast stain and don't even bother to look at them. I was very nearly fired after only a short time in my present position when the Medical Director found out that I was colorblind. He couldn't see how I could possibly do histology. I convinced him to give me a chance and 28 years later I am still here. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Tuesday, March 13, 2012 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Color Blindness Testing Laurie asks: Does anyone know of any (free) online testing for color blindness? Does anyone have an alternate method that they have used to satisfy CAP requirements? Here are some online Ishihara plates quickly found through Wikipedia: http://offsetpressman.blogspot.com/2009/10/color-blindness-test-for-printers.html There are many others. Two reservations: Color monitors don't reproduce colors with sufficient accuracy. And an online test may not satisfy bureaucrats. You'd have to ask. I have normal color vision, but I've been interested in color blindness among pathologists for about 50 years. I know of only one major problem, finding red acid-fast bacilli in a blue-background stain. This problem should be solved by banning light-microscopic acid-fast stains, something the CAP should have done years ago. I don't like the implication of color-blindness testing in the absence of a significant problem. Can somebody lose their job, or not get hired in the first place, because of color-blindness? It may be Good Management, but it's not good medicine or good social policy. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Destain/Restain
We do not destain he slides. We just take them back to water and stain right over top of the he. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Virginia Chladek Sent: Monday, March 05, 2012 3:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Destain/Restain I need your help- My doc needs me to destain an he slide to run a ck5 on the Bond3, how can I do this without the tissue falling off? I ran the slide back down from xylene to water and destained, then introduced the slide to bond wash before starting the protocol in bond wash again and lost the tissue. Any suggestions? Thanks! Maggie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Agar for cell blocks
We use soy agar slants that we get from our Micro department. We just melt one as needed. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Angelo Sent: Tuesday, February 28, 2012 5:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Agar for cell blocks Is anyone using an agar for their cell block preparation that they can recommend? ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ribbon won't stay on knife
I use my forceps to round over the top edge of the block when this happens. If you ever have trouble getting a ribbon to form because the sections do not stick together, you can also use the forceps to straighten the bottom edge of the block. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jerry Ricks Sent: Wednesday, February 15, 2012 6:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ribbon won't stay on knife Sometimes while cutting 5 micron paraffin sections... I turn the wheel once and a nice section gets cut and lays down on my blade. When I turn the wheel again, as the knife holder is traveling Upwards, the top of my paraffin block will grab the ribbon and lift it off of the microtome blade, ruining my ribbon. Any ideas what is going on, how to fix it. Today I'm using my old trust Spencer 820 microtome, but it also sometimes happens on my Leica RM2125. Thanks, Jerry Ricks Research Scientist University of Washington Department of Pathology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC and negative controls?
Hi All, We have one Ventana XT and often exceed the slide capacity. Our pathologists no longer want us to run negatives and will use internal negatives instead. Is this common practice? Thanks. I appreciate any feedback. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: IHC and negative controls?
Thanks to everyone for the quick responses. I am looking at ANP.22580 rev 12/12/2006 and it clearly talks about a separate section of patient tissue... but just a little further it talks about using internal negative controls (The type of negative tissue control used (i.e., separate sections, internal controls or multitissue blocks) should be specified in the laboratory manual (refer to ANP.22250).) I did not see anything useful in ANP.22250. We are JCAHO accredited not CAP but I cannot find any JCAHO discussion on the subject. Personally, I believe that we should run separate negative controls for each case. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Thursday, February 16, 2012 10:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC and negative controls? Hi All, We have one Ventana XT and often exceed the slide capacity. Our pathologists no longer want us to run negatives and will use internal negatives instead. Is this common practice? Thanks. I appreciate any feedback. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: H. pylori
Dako concentrate on the Ventana XT. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, February 14, 2012 10:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H. pylori Happy Valentines Day! Our lab is looking into ordering H. pylori now and I was wondering what clone, or whose antibody everyone is using. As always, thanks for your responses. I know I can always count on this group to offer opinions and suggestions :) Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] medi tech users
We do not. We order all of our specimens. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Adey Sent: Wednesday, January 11, 2012 5:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] medi tech users Hello:Are any of the medi tech users having the OR order the pathology specimens into the system before they arrive in the lab?:)Sheila ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: H. Pylori - IHC
We get beautiful staining with Dako's concentrate on the Benchmark XT. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of beth@hcahealthcare.com Sent: Wednesday, November 16, 2011 5:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H. Pylori - IHC Does anyone have recommendations for a good vendor for H.Pylori for IHC? We have tried many, but our pathologists complain that they are not specific for H.Pylori and that all gram negative bacteria are staining. Currently we are not running this but our pathologists are interested in it. Thanks! Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 mailto:beth@hcahealthcare.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Regarding MOHS....
Hello all, I have some questions for the community sized hospital that are doing MOHS. I would appreciated any input on the following questions: Do you have people specifically dedicated to performing MOHS? About how many specimens per patient do you receive? Are MOHS procedures performed STAT? Do you have more than one person working on MOHS (one to cut one to stain)? Again, thanks in advance for you input. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Cold Plates - Source?
Check out the Histo-Cool trays http://www.marketlabinc.com/product.asp?P_ID=4854 You can get them from several places. They stay cold all day. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Friday, October 07, 2011 4:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cold Plates - Source? Hello All, Does anyone know where Tissue-Tek cold plates can be purchased? They are plastic, black on the bottom, white on top, gel filled, for chilling blocks. I got them from Cardinal Healthcare a couple of years ago, but I don't see it in their catalog now. Or, if no longer available, a comparable other product? I know I can use a pan of ice, but I prefer something like this if available. Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Slide/Block Retention
Are you including Cytologies as well? Cytology has the requirement of a five year look-back. We keep all surgical and cytology blocks and slides for 10 years. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J Sent: Monday, October 03, 2011 9:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide/Block Retention Hello Histonet, What policy is everyone following for slide and block retention? We are not CAP, however we currently keep slides and blocks for 10 years. My director wants to decrease that period to 2 years, which is the Joint Commission standard. I would like to keep 10 years because we frequently are asked to send slides for consult and review that are several years old. It is also helpful when looking for hard-to-find control tissue. However these reasons are apparently not sufficient. I'm leaning towards keeping the 10 year policy, but I need additional justification. Or does anyone think the 2 year period is sufficient? Thanks, Mike Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdess...@wvhcs.org mailto:mjdess...@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Controls with patient specimen on same slide
We put patients and controls on the same slide. We used to use the slides with the red box for the control but encountered staining issues. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, July 19, 2011 3:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Controls with patient specimen on same slide Hi, I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this? If you're not, why not? Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Leica Service Technician
It is really just a freezer with a microtome in it. Do you have an on-site refrigeration guy that could look at it? I have used our in-house guy a time or two. Ours is pretty simple and does not have all of the electronics that you may have on the Leica but it is worth a shot. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amanda Madden Sent: Wednesday, July 20, 2011 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Service Technician Hi Histonetters! A few months back I emailed regarding a service contract through Leica for our CM 3050S, and (unfortunately?) we chose not to purchase one. This week we have had a serious issue with it... the specimen head and arm is covered in frost, and the object temperature sensor is reading ## instead of a temp. In any case, we called Leica and asked for a service call, but it is extremely expensive and they couldn't give us an estimate of when they will be here because cryostats used for clinical applications have priority over those, like ours, that are used for research. Understandable, but frustrating nonetheless. So my question is: does anyone know of a good, reputable cryostat service technician (who is authorized by leica, if possible) that is located in the Boston, MA area? Any help would be greatly appreciated! Thanks in advance, Amanda ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Controls with patient specimen on same slide
Each case gets one positive control per antibody. If, for example, I run a bone marrow bx and aspirate, I use one positive control for both slides. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, July 20, 2011 9:19 AM To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls with patient specimen on same slide All of these responses are great. So here's a follow up question. Do you place a control tissue on EACH slide if you have multiple blocks for a case, or just on one of the slides? -Original Message- From: Richard Cartun [mailto:rcar...@harthosp.org] Sent: Tuesday, July 19, 2011 6:40 PM To: histonet@lists.utsouthwestern.edu; Rathborne, Toni Subject: Re: [Histonet] Controls with patient specimen on same slide We do not put our positive control tissue on the test slide; we run batch controls. Many of the unstained slides (breast, GI, and prostate biopsies) that we use for IHC testing are cut in our Histology Laboratory as part of a part-type slide protocol. For example, we cut 7 slides, 2 sections on each slide, for breast biopsies and stain #1, 4, and 7 with HE, and then use (if needed) #2, 3, 5, and 6 for IHC. Therefore, it would be very difficult for us to place the positive control tissue on the same slide. In addition, I receive a lot of consult cases from other hospitals where they send us unstained slides for testing. Once again, it would be difficult to place the positive control tissue on the same slide and it would slow us down in terms of starting those slides once they arrive. However, I think the main reason we don't pursue putting the positive control tissue on the same slide is the fact that it would consume an enormous amount of control tissue. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Rathborne, Toni trathbo...@somerset-healthcare.com 7/19/2011 3:27 PM Hi, I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this? If you're not, why not? Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - This message was secured by ZixCorp(R). CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Remote Alarm for Processor
We have an alarm system that monitors all of the refrigerators and freezers in the lab and our processor is tied into that. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, July 13, 2011 9:14 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Remote Alarm for Processor Do you use a remote alarm on your processor? If so, would you tell me what brand/model/manufacturer and how it works (keeping in mind that I am Electronically Challenged)? I run a Leica ASP300 in a new building with only digital phone lines and zero cell phone reception inside the building. I can't think of anything else that would complicate this issue more than that! Any help out there? Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] In search of a cryostat...
Hello all, I am looking to budget for a new cryostat and was wondering what others are using. I would like one that is safe, well lit, roomy, and has some sort of on-board decontamination routine. All input appreciated. Thanks in advance. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] p16 Protocol....
Hello all, I was wondering if anyone would be willing to share a protocol for p16 (Biogenix pre-dilute) antibody on the Benchmark XT. I'm having a little trouble getting anything to stain. Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Temperature monitoring system
All of our laboratory refrigerators and freezers are monitored by a system from Rees Scientific. In Histology we only have one refrigerator that stores our antibodies and we check and record the temperature daily. We do have our tissue processor connected to this alarm system since there is no in the vicinity after 4pm. It may be more that you want or need but they can probably tailor a package to your needs. You can find them online. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Tuesday, June 14, 2011 9:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Temperature monitoring system We are looking for a system to monitor a couple of our lab refrigerators containing expensive antibodies and probes. Can anyone recommend a small, basic system? Thank you in advance. Sheila Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: printed requisitions
We still require a paper req with all specimens. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, June 01, 2011 4:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] printed requisitions Does everyone still receive printed requisitions with your surgical specimens? How many of your OR's have a printer in each room? Our OR has a shared printer for all of the procedure rooms, but would like to have a printer for each OR suite. Our IT department is saying there would be problems and has suggested that we go paperless. I see many problems arising from this. If anyone is doing this successfully, I'd be very interested in hearing how you accomplished it. Toni Regards, Toni Rathborne Pathology Supervisor Somerset Medical Center 110 Rehill Ave. Somerville, NJ 08876 CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] back up power for VIPs?
Hello Sheila, We are using the Smart-UPS 2200 from APC. We are also on a dedicated emergency line through our facility. We are running the Sakura VIP 5. I cannot give you any other specifics about the UPS since I can't get to the back of it but it was recommended and installed by our biomed people. Seems to work fine. That model has been discontinued but this is the recommended replacement... APC Smart-UPS 2200VA USB Serial 120V More Images APC Smart-UPS, 1980 Watts / 2200 VA,Input 120V / Output 120V, Interface Port DB-9 RS-232, SmartSlot Includes: CD with software, Smart http://www.apc.com/products/resource/include/techspec_index.cfm?base_sku=SUA2200 Hope this helps... Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Adey Sent: Friday, May 27, 2011 9:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] back up power for VIPs? Hello everyone: We are experiencing lots of quick power outages at work that keeps setting the processors into power outage alarms. Can anyone recommend a back up power product. I was told we can't put them on battery back up b/c of their power requirements??? They are on emergency power. Thanks for your help in advance. :) Sheila ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cassette/Slide labelers.... again....
Hello all, Is anyone using the ID/Positive system from Data General? Pros? Cons? Any info would be helpful. Thanks! Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Grossing Station Recommendation
Grosslab Senior here as well. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sean McBride Sent: Monday, March 21, 2011 2:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Grossing Station Recommendation Colleagues, We are looking to replace our current pathology grossing hood with a new station, so I am looking for recommendations. Thanks in advance for all of your wonderful advice. Best regards, ~Sean McBride Scientific Specialist Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-915-1683 (m) 412-268-8275 (fax) smcbr...@andrew.cmu.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: microtome safety
Both. Fingers to lift one end of the ribbon as I cut it and then with the other hand I use a small brush to lift the other end from the microtome. Allows me to stretch and smooth the ribbon as I lay it out on the water bath. Some others here us forceps, picks, etc. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Tuesday, March 08, 2011 8:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] microtome safety Morning all! I need some quick responses to this question: do you use your fingers or an instrument of some sort to pull your paraffin ribbons off the block when sectioning? For those that do not use their fingers, what do you use? If forceps, are these the typical lab forceps or a special type? Thanks so much! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartl...@cdc.hhs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] PAS with Diastase Digestion
Spit for 5 minutes old school Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Erin Sarricks Sent: Wednesday, February 09, 2011 7:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAS with Diastase Digestion Hi Histonet- I recently ran a PAS/D stain and had some issues with it. Both with and without slides came out looking the same so I'm guessing my digestion step didn't work! I used a Malt Diastase solution (0.5g to 500mL water) for my digestion. This is the procedure I used: 1.Deparaffinize and hydrate to water. 2.Place the sections labeled “with” in diastase solution preheated to 37˚C for 1 hour. Hold the sections labeled “without” in distilled water. 3.Wash in running water for 5 minutes 4.Place all section (with and without) in 0.5% periodic acid solution for 5 minutes 5.Wash in 3 changes of distilled water 6.Place in Schiff reagent for 15 minutes 7.Wash in lukewarm tap water for 5 minutes (immediately sections turn dark pink color). 8.Counterstain in Mayer’s Hematoxylin for 3 minutes. 9.Wash in tap water for 10 minutes 10. Dehydrate starting with 95% ETOH, clear, and coverslip. I am wondering if my solution possibly got too warm in the oven and hindered the enzyme activity, or is it possible I left it in too long? Any tips would be much appreciated! Oh, and I have about 300 slides to stain, so spitting on them is my last last last resort! Haha! Thanks in advance for all your help! Erin Sarricks, HT (ASCP) Histology Laboratory Technician USAMRICD Comparative Pathology Branch Office: Bldg E-3081 Room 178 E-mail: erin.p.sarri...@us.army.mil ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Saponin Technique
I don't know about the procedure you have but ours only uses a 1% solution of saponin, a 3% solution of calcium gluconate, and a balanced salt solution. We get the balanced salt solution from our pharmacy. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Monday, October 25, 2010 11:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Saponin Technique From: Hannen, Valerie Sent: Monday, October 25, 2010 11:39 AM To: 'histo...@list.utsouthwestern.edu' Subject: Saponin Technique Hi folks.. I am hoping someone out there in Histo-land can help. One of our Pathologists is asking us to check into doing the Saponin Technique to lyse the red cells in some of our cytology specimens. She gave a copy of one of the pages from one of her manuals, and it shows that in order for us to do this we would need to buy alot of reagents that we currently do not have. Being that we would probably not be doing this technique very often, and not sure how cost effective it will be for us to buy the reagents, we are wondering if anyone knows of a commercial product that we can buy that would be a cheaper way out. Thanks in advance!! Valerie Hannen, Histotechnologist Parrish Medical Center Titusville,Florida ** This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: bar coding specimens, slides, blocks
Also interested in this... Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, September 08, 2010 4:08 PM To: histo...@pathology.swmed.edu Subject: [Histonet] bar coding specimens, slides, blocks I am looking for a vendor that has the capability to barcode specimens, blocks and slides. Also if it can interface with Meditech client server 6.0 it would be a plus. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's WaySlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.archildrens.org ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Lung biopsies - Question
We cut 6 extra up front. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, August 26, 2010 4:54 PM To: Histonet Subject: [Histonet] Lung biopsies - Question How many labs cut extra tissue sections (or tissue curls) for molecular testing (KRAS, BRAF, EGFR, etc.) on lung biopsies up front (at the time of HE sectioning)? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Microtome alignment
I assume you are referring to a small carpenter's square. That will only allow you to square up the chuck from top to bottom. It would not work for left and right adjustments. Marketlab sells an alignment tool that they claim will work for multiple microtomes. I have not tried it though. It lists for $775. I have considered having a piece of steel welded to an old knife holder and just adjust the chuck until it is flat against the steel. Should work and won't cost $775. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Wednesday, August 18, 2010 7:00 AM To: WILLIAM DESALVO Cc: histonet Subject: Re: [Histonet] Microtome alignment A while back I remember someone suggestion something like a right angle device that carpenters use. It's basically just a piece of metal that is a right angle triangle that you put up against the chuck and on the knife mount. Then you align the chuck so it is a a right angle to the knife mount. It looks like this:l\ l \ l\ This is my best attempt at computer drawing. l __ \ I don't thing they cost very much, much less that $700. Paula :-) On Tue, Aug 17, 2010 at 8:05 PM, WILLIAM DESALVO wdesalvo@hotmail.comwrote: Since you have older microtomes, I suggest using an alignment block at each microtome instead of purchasing the alignment tools. The tools can be found on the web ttp://www.grale.com.au/products/view/804 , but they can be expensive (as much as $700.00 each). If you have more than one manufacturer for your microtomes, you will need to purchase one for each brand. Try using your largest embedding mold and make a blank block for each microtome. This can bee done first thing each morning. Use the block to align the chuck each morning before cutting. If you see drift throughout the day, add one or more checks during the day. Making a fresh block each day gives you a good standard and keeps the variation down. I also suggest you look at your embedding method and make sure you have a standardized procedure for all tissue types for orientation of tissue and exact placement in the mold. Embed your tissue on one plane with as little paraffin as possible on the bottom of the mold. Reducing variation at embedding will greatly assist you in reducing the amount of facing required to start producing sections and also reduce the need to align the chuck to the block/tissue. William DeSalvo, B.S., HTL(ASCP) Chair, NSH QCC Prodcution Manager, Sonora Quest Laboratories From: sharon.davis-dev...@carle.com To: histonet@lists.utsouthwestern.edu Date: Tue, 17 Aug 2010 14:16:26 -0500 Subject: [Histonet] Microtome alignment We are having a continuing issue of too much tissue being cut off when facing off a block for recuts. We have tried a couple of different methods for aligning our microtomes without much success. Does anyone out there have any advice on how to properly align them and what tool to use? Also, how often do you perform this re-alignment? The majority of our microtomes are older so more wear and tear and things move out of place more often. Any help or suggestions will be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-dev...@carle.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Paula Sicurello 6 of 6 Duke Healthcare System EM Lab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure
[Histonet] Full-time day Job open in Central Ohio..
We have and opening for a full time day shift histotech. We are a small but progressive hospital of around 250 beds in Newark, Ohio (about 30 miles from Columbus). If interested you may apply through our website (www.lmhealth.orghttp://www.lmhealth.org/) Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Prepared Solutions
Long, long ago, we made everything up (mostly from powders), we kept all the procedures written on recipe cards filed alphabetically in a recipe box and it worked very well. About the only thing we make up anymore is formalin, and graded alcohols. I have a procedure for the formalin but none for the alcohols. We labeled the working solutions with the chemical name, percentage, and a shelf life date. Dry powders lasted forever (many did not even give an expiration) so we just fabricated a reasonable shelf life date. I also think it is advisable to list and spell out the name and concentration of every ingredient used. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Brandi Higgins Sent: Friday, June 25, 2010 12:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Prepared Solutions Hello All, I work in a small lab where we purchase most of our chemicals already prepared. The only solutions we routinely prepare ourselves are our acid alcohol and acid water for our staining procedures, Carnoy's Fixative (since it needs to be fresh) and our 50%, 70% etc alcohol solutions for our Pap stain and for our processing machine. I would also like to start preparing our 1% and 3% acetic acid solutions instead of purchasing them since I have glacial acetic acid on hand anyway for our Carnoy's Fixative (not that the acetic acid acid solutions are expensive but every dollar counts, plus the shipping charges always shock me). My questions are 1 - in your labs, do you have a policy with instructions on how to prepare each solution? (eg 1 ml acetic acid diluted with water to 100 ml to prepate 1% Acetic Acid Aq and is it neccessary to say 500 ml water and 500 ml reagent alcohol to form 50% alcohol) 2 - when you label the solutions you prepare, do you transfer the lot numbers and expiration dates of the chemicals that were used to make the solutions onto the new chemical bottles and use the expiration date of the concentrated chemical as the expiration date of the prepared chemical? Thanks in advance for all input. Im always amazed at how fast, how many, and how thorough/helpful the responses are! Brandi Higgins, BS, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Retaining empty jars
CAP retention guidelines say that wet tissues are to be retained for 2 weeks after final signout. We keep all containers, empty or not, for the 2 weeks then strain and discard them in biohazard trash. We have a large cabinet and designate a shelf for each day. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rosa Fields Sent: Wednesday, June 23, 2010 9:09 AM To: mtitf...@aol.com; histo...@pathology.swmed.edu Subject: RE: [Histonet] Retaining empty jars We also retain ours; for about 2 weeks. I have plastic bins labeled Monday 1/ Monday 2.. ect.. that way I don't have to search through all of them if I have a question about a case. Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfie...@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mtitf...@aol.com Sent: Wednesday, June 23, 2010 7:26 AM To: histo...@pathology.swmed.edu Subject: [Histonet] Retaining empty jars Rhonda Hartz asks if anyone else retains empty jars after grossing. We too retain our empty jars after grossing for about a month. We keep them in a red biohazard trash bag. It pays dividends. If once the case has been signed out the physician/surgeon complains that they removed more biopsies than we listed in the report, or submitted more jars, etc, we can go back and check. On rare occasions we have found tiny pieces of tissue in the jars, or hidden in the grooves of the lid. These enquiries most often happen with tiny pieces of tissue, of course, like GI biopsies. We have the physicians write on the label on the jar what is inside it (like everyone else I guess) as well as listing it on the surgical pathology slip. You can also go back and check what they wrote down. Afterwards the jars are incinerated as they have all that HIPPA related stuff on the labels. Michael Titford USA Pathology Mobile AL USA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] number of slides
We do 2 levels on all GIs, 3 on all needle bx, 3 on cervical, and 2 on bone marrows. We also do special stains up front on the bone marrows, H Pyloris, prostates, etc. We tend to cut a ton of extra slides that we just throw away. I don't like it but I don't think there's any way around it. Probably like most places, we get more and more smaller and smaller biopsies so it's better to keep a few extras than to try to go back later. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Tuesday, March 23, 2010 6:08 PM To: anita dudley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] number of slides We do three levels on all diagnostic biopsies. Liver and Kidney also get upfront special stains, as do gastric biopsies (h pylori) We did cut down on extra unstained slides since we were discarding most of them. As far as stopping levels, my pathologist thinks it goes against standard of care. As for prostate biopsies, they are so small any more, that we are thinking o cutting 4 slides staining 1and 4 for HE and holding 2 and 3 for possible IHC. We are finding that when we have to go back there is minimal tumor for IHC demonstration. Susan T. Paturzo Thomas Jefferson University Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4061. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Plants in the Histology Lab
I don't know about any list but I can tell you that spider plants grow like crazy they like the formalin. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Shelly Christenson Sent: Thursday, October 22, 2009 12:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Plants in the Histology Lab Some time back I took a workshop on Plants in the Histology Lab. Some where I have the list as to which plants are the most beneficial in the lab and which chemical fumes they like best. I don't remember who did the workshop. I will have to do a little hunting for the paper, but My Mother-IN-Tongues really like the lab's fumes they are 3 to 4 feet tall and I've divided them 3 times now. Shelly Christenson HT(ASCP) Veterinary Diagnostic Lab. Kansas State University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
