Re: [Histonet] Lab Coats and OCT
We get our freezing medium from TBS. it is called TFM (tissue freezing medium). Lately it has become much less viscous, one of the reasons I liked it was that the viscosity allowed me to orient my tissue a little easier. The packaging also changed claiming "same TBS Product, Great New Packaging". Has anyone else noticed the change, or did I just get a bad lot? Thanks for any information, Kathy Katherine S Walters Histology Director Central Microscopy Research Facility University of Iowa 76 Eckstein Medical Research Building 431 Newton Road Iowa City, Iowa 52242 319-335-8142 Facility Website: http://cmrf.research.uiowa.edu/ -Original Message- From: Rathborne, Toni via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, February 18, 2016 10:31 AM To: 'Seeley, Heather' Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Lab Coats and OCT Heather, We are currently using a lab coat made by White Knight, which is fluid resistant. I don't know if it's any cooler, but they do protect what you're wearing under it. Regarding the OCT. We also get our from Sakura, and have not noticed any change to the consistency. Is this happening with all lot numbers, or only one? I would check with Sakura. There may be a recall that you're not aware of. It might also have something to do with temperature/storage conditions. Toni -Original Message- From: Seeley, Heather via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, February 18, 2016 11:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Lab Coats and OCT Hello All, Now that the hospital is enforcing us to wear lab coats all the time, wondering if anyone has suggestions for some that aren't too hot, the ones we have now are making us sweat! Also, we have been having issues with our OCT, we currently use the Sakura OCT and have use it for years. Within the last 6 months or so it has become like glue on the chucks even if we let it soak in hot water and scrub with a brush, it just doesn't want to come out! If anyone has any suggestions on brands to try we would appreciate it! Thanks! HEATHER SEELEY, HT(ASCP) Histotechnologist 803-985-4676 OFFICE 803-327-7598 FAX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] ammonium bromide in fixative
Thank you for such a detailed response. It is doubtful I would have found this information without you. The National Histology Society online learning center is one of my study resources, this is where the question came from. They also include several mercuric fixatives that we would never use in our lab in this day and age. Perhaps the material has not been updated for a number of years. I have not yet heard from any recent exam takers about the content of old techniques. I find them interesting, but don't know how much time to devote to them. Katherine S Walters Histology Director Central Microscopy Research Facility University of Iowa 76 Eckstein Medical Research Building 431 Newton Road Iowa City, Iowa 52242 319-335-8142 Facility Website: http://cmrf.research.uiowa.edu/ From: John Kiernan [mailto:jkier...@uwo.ca] Sent: Friday, June 12, 2015 11:58 PM To: Walters, Katherine S; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] ammonium bromide in fixative Formalin with ammonium bromide is the fixative prescribed for traditional silver and gold techniques for staining neuroglial/x cells in free-floating frozen sections. Adding ammonium bromide lowers the pH of the fixative because of a chemical reaction with formaldehyde. An Argentinian histologist/x called Lascano showed in the 1940s that any sufficiently acidified formaldehyde solution (pH 1.5) was just as good as FAB for Cajal's gold-sublimate (astrocytes) and for silver carbonate methods (oligodendrocytes, microglia). The reaction that lowers the pH is 4NH4+ + 6HCHO --- C6H12N4 + 6H2O + 4H+ (Subscripts and superscripts in this equation will not be honoured in this email!) The 4H+ lowers the pH. The other product, C6H12N4 is hexamethylenetetramine, also known as hexamine in Britain and methenamine in the USA, and used in various histochemical staining methods, notably Grocott's method for fungal cell-walls in sections of animal (including human) tissues. The bromide (Br-) ion of NH4Br is irrelevant, in the FAB fixative and also in Globus's pretreatments (dilute ammonia followed by dilute hydrobromic acid). Globus's bromuration treatment was applied to frozen sections of pieces of CNS that had been fixed in non-acidified formalin. The references for Lascano's work are: Lascano, E.F. (1946a). Influencia del pH en la impregnacion argentica del tejido nervioso. Archivos de la Sociedad Argentina de Anatomia Normal y Patologica 8:105-114. Lascano, E.F. (1946b). Importancia del pH en la fijacion del tejido nervioso. Creacion artificial de fijadores/x tipo formol-bromuro y formol-nitrato de urano de Cajal. Archivos de la Sociedad Argentina de Anatomia Normal y Patologica 8:185-194. Lascano, E.F. (1946c). Influencia del pH del fijador en la coloracion argentica del tejido nervioso. Archivos de la Sociedad Argentina de Anatomia Normal y Patologica 8:272-276. Not everyone agreed with Lascano! Polak, M. (1948). Sobre la importancia del bromuro de amonio de la solucion fijadora de Cajal en la impregnacion argentica del tejido nervioso. Archivos de la Sociedad Argentina de Anatomia Normal y Patologica 10:224-234. Do you really need this information to pass your HTL qualifying exam? It isn't easily found in books or with Google Scholar. I came across these papers quite by chance when looking over some old journals that UWO's library had set aside for throwing out (Gasp!), about 1980. Yes, they had hit a new low! Their services have, however, been exceptionally good for the last 10-15 years. Does anyone still use either Cajal's method for astrocytes or traditional silver carbonate methods to stain oligodendrocytes and microglia? They are difficult for several reasons, take up time, and require an old-fashioned freezing microtome to cut and collect the rather thick sections that are needed. Reliable antibodies for immunostaining glial cell-types have been available for many years, and they work on any kind of section. You need some thickness to appreciate the 3D shapes of astrocytes and oligodendrocytes, however they are stained. There's plenty of histo-history in the traditional neuroglia stains, which defined the cell-types for identification by electron microscopy and immunohistochemistry. John Kiernan Anatomy Cell Biology University of Western Ontario London, Canada = = = On 11/06/15, Walters, Katherine S katherine-walt...@uiowa.edumailto:katherine-walt...@uiowa.edu wrote: Hi all, I am studying to take the HTL certification test and ran across a reference to Formalin Ammonium Bromide. I see that it is very good for central nervous tissue fixation, it must be made fresh and that its pH is 1.5. Does anyone happen to know the reason for ammonium bromide in this fixative? I have been looking online and this has not been explained. Also, has anyone taken this test lately? I am curious as to how much old techniques, such as mercuric fixatives will be included
[Histonet] ammonium bromide in fixative
Hi all, I am studying to take the HTL certification test and ran across a reference to Formalin Ammonium Bromide. I see that it is very good for central nervous tissue fixation, it must be made fresh and that its pH is 1.5. Does anyone happen to know the reason for ammonium bromide in this fixative? I have been looking online and this has not been explained. Also, has anyone taken this test lately? I am curious as to how much old techniques, such as mercuric fixatives will be included? Thank you, Katherine S Walters Histology Director Central Microscopy Research Facility University of Iowa 76 Eckstein Medical Research Building 431 Newton Road Iowa City, Iowa 52242 319-335-8142 Facility Website: http://cmrf.research.uiowa.edu/ Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Staining net dishes for brain sections
Yes, I love them! -Original Message- From: Gayle Callis [mailto:gayle.cal...@bresnan.net] Sent: Wednesday, May 13, 2015 12:17 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Staining net dishes for brain sections Out of curiosity and having received an email from Brain Research Laboratories about this new item, has anyone tried the staining net dishes for free floating brain or other tissue sections? I thought this a clever, useful device to keep the sections from physical damage without having to lift the sections in some way. Gayle Callis HTL/HT/MT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] phospho-Akt antibody
Dear Histo-netters, I am trying to work up a phosphor-akt antibody (Cell Signaling #3787) for my FFPE blocks of mouse uterine tubules. Fixation is in Z-fix for 48 hours. So far I have tried HIER with both microwave retrieval and a pressure-cooker. I am using DAKO's En-vision plus rabbit secondary HRP conjugate and reacting with DAB. The results are less than impressive, although I am seeing some low level staining at 1:50. Has anyone used this antibody with better success? Thanks for any help in this matter. Katherine Walters Histology Director Central Microscopy Research Facilities 85 Eckstein Medical Research Building University of Iowa Iowa City, Iowa 52242-1101 phone: # 319-335-8142 fax: # 319-384-4469 katherine-walt...@uiowa.edu www.uiowa.edu/~cemrf ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] purple spots
Hi All, I recently did an Oil-Red-O stain using my usual procedure with formaldehyde vapor fixation, Harris hematoxylin, and finally Oil-Red-O. I was staining some frozen diseased aortic mouse tissue. This time the stain resulted in some dense purple spots in the tissue. I had not seen this before. I have posted pictures on the www.histonet.org website. The files are named low mag 2c, 2b, 2d, and 2e. The last three are a higher magnification of the first picture. I was hoping someone can tell me what these spots are. I suspect that they are simply necrotic tissue, but I would like the opinion of experts. Thanks for taking a look! Kathy Katherine Walters Histology Director Central Microscopy Research Facilities 85 Eckstein Medical Research Building University of Iowa Iowa City, Iowa 52242-1101 phone: # 319-335-8142 fax: # 319-384-4469 katherine-walt...@uiowa.edu www.uiowa.edu/~cemrf ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Alcian blue alizarin red double stain
I would also be interested in the protocol. Katherine Walters Histology Director Central Microscopy Research Facilities 85 Eckstein Medical Research Building University of Iowa Iowa City, Iowa 52242-1101 phone: # 319-335-8142 fax: # 319-384-4469 katherine-walt...@uiowa.edu www.uiowa.edu/~cemrf -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas, Nancy Sent: Tuesday, April 20, 2010 12:49 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Alcian blue alizarin red double stain Our lab has a request for alcian blue/alizarin red staining on mouse kidney teratomas. This will be done on FFPE slide mounted sections. In the past, we have done skeletal staining using these stains which also involved all of the clearing steps using potassium hydroxide.Has anyone done this stain (minus all of the clearing steps) on paraffin sections? If so, could I get a copy of your protocol? Thank you, Nancy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Arcturus Laser Capture Microdissection (LCM) Systems
Molecular Devices bought them. Here is their website: http://www.moleculardevices.com/pages/instruments/microgenomics.html Hope this helps, Kathy Katherine Walters Histology Director Central Microscopy Research Facilities 85 Eckstein Medical Research Building University of Iowa Iowa City, Iowa 52242-1101 phone: # 319-335-8142 fax: # 319-384-4469 katherine-walt...@uiowa.edu www.uiowa.edu/~cemrf -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kimberly Tuttle Sent: Tuesday, February 16, 2010 11:16 AM To: histonet Subject: [Histonet] Arcturus Laser Capture Microdissection (LCM) Systems Does anyone know who to contact to get this instrument serviced? It seems the company is no longer in business. The phone numbers I have dont work, and the website is no longer there. I found a company online who repairs them, but they are in Massachusetts, I am in Maryland so that would be my last resort. Thanks Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Arcturus Laser Capture Microdissection (LCM) Systems
GEEZ hard to keep track! Katherine Walters Histology Director Central Microscopy Research Facilities 85 Eckstein Medical Research Building University of Iowa Iowa City, Iowa 52242-1101 phone: # 319-335-8142 fax: # 319-384-4469 katherine-walt...@uiowa.edu www.uiowa.edu/~cemrf -Original Message- From: Victor Tobias [mailto:vic...@pathology.washington.edu] Sent: Tuesday, February 16, 2010 11:37 AM To: Walters, Katherine S Cc: Kimberly Tuttle; histonet Subject: Re: [Histonet] Arcturus Laser Capture Microdissection (LCM) Systems And they have since been bought by *Danaher* company_profile.aspx?CompanyId=1002531 Completes Acquisition of *AB SCIEX* company_profile.aspx?CompanyId=1009895 and *Molecular Devices Corporation* company_profile.aspx?CompanyId=3170 (MDCC http://investor.biospace.com/biospace?Page=QUOTETicker=MDCC) 2/1/2010 WASHINGTON, Feb. 1 /PRNewswire-FirstCall/ -- Danaher Corporation announced today that it has completed the previously announced acquisition of AB SCIEX and Molecular Devices. AB SCIEX is a leading designer and manufacturer of mass spectrometers, highly sensitive and sophisticated instruments used by researchers and clinicians to identify and quantify specific molecules in complex samples. Molecular Devices supplies high-performance bio-analytical instrumentation systems and consumables that accelerate and improve research productivity and effectiveness in life science research and drug discovery. Danaher Corporation Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 vic...@pathology.washington.edu 206-598-2792 206-598-7659 Fax = Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Walters, Katherine S wrote: Molecular Devices bought them. Here is their website: http://www.moleculardevices.com/pages/instruments/microgenomics.html Hope this helps, Kathy Katherine Walters Histology Director Central Microscopy Research Facilities 85 Eckstein Medical Research Building University of Iowa Iowa City, Iowa 52242-1101 phone: # 319-335-8142 fax: # 319-384-4469 katherine-walt...@uiowa.edu www.uiowa.edu/~cemrf -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kimberly Tuttle Sent: Tuesday, February 16, 2010 11:16 AM To: histonet Subject: [Histonet] Arcturus Laser Capture Microdissection (LCM) Systems Does anyone know who to contact to get this instrument serviced? It seems the company is no longer in business. The phone numbers I have dont work, and the website is no longer there. I found a company online who repairs them, but they are in Massachusetts, I am in Maryland so that would be my last resort. Thanks Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] plasminogen activator inhibitor 1 (PAI 1) antibody
Has anyone had any experience with this antibody? There are many companies selling them and I'm not sure which would be best for my paraffin-embedded human tissue? Katherine Walters Histology Director Central Microscopy Research Facilities 85 Eckstein Medical Research Building University of Iowa Iowa City, Iowa 52242-1101 phone: # 319-335-8142 fax: # 319-384-4469 katherine-walt...@uiowa.edu www.uiowa.edu/~cemrf ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHCCRG; RE: Use of immuno cal or other gentle formic acid decal solution for immuno specimens
Would you please post this procedure? Thanks. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Reynolds,Donna M Sent: Friday, September 18, 2009 8:20 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] IHCCRG; RE: Use of immuno cal or other gentle formic acid decal solution for immuno specimens From: Reynolds,Donna M Sent: Friday, September 18, 2009 8:01 AM To: 'ih...@googlegroups.com' Subject: RE: Use of immuno cal or other gentle formic acid decal solution for immuno specimens I have attached a decal procedure we have used for a long time. We use it for mouse bones mostly. But we have also used it for Human bone marrow bx. We have beautiful IHC's using this method. Donna Reynolds HT(ASCP) Chief Histology Laboratory Dept. Cancer Biology U.T. M.D. Anderson Cancer Center Houston, Texas 713-792-8106 From: ih...@googlegroups.com [mailto:ih...@googlegroups.com] On Behalf Of Van Eyck, Deb Sent: Tuesday, September 15, 2009 10:30 AM To: histonet@lists.utsouthwestern.edu Subject: [IHCRG] Use of immuno cal or other gentle formic acid decal solution for immuno specimens Hi all, Would someone share with me their times and procedure for formic acid decal of bone marrows for IHC. Thanks Deb Deb Van Eyck CT(ASCP) QIHC Waukesha Memorial Hospital 725 American Avenue Waukesha,WI 53188 262-928-2112 This information is confidential and intended solely for the use of the individual or entity to whom it is addressed. If you have received this email in error please notify the sender or our Customer Support Center at (262) 928-2777. We have scanned this e-mail and its attachments for malicious content. However, the recipient should check this email and any attachments for the presence of viruses. ProHealth Care accepts no liability for any damage caused by any virus transmitted by this email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] EM processing
We make our own from commercially available components. It takes on an average 3-4 days from fixative to cured block. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histopa...@aol.com Sent: Friday, August 28, 2009 10:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] EM processing Does your lab make their own plastic resin to process and embed specimens or do you buy it commercially? What is the average time it take to process a specimen? Thanks in advance to all who respond. P.Eneff OU Medical Center Oklahoma City, OK ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] NSH Meeting Hotels
Good to know, but when I booked they had no trouble finding National Society of Histotechnology. I'd've never come up with CION!! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Thursday, August 13, 2009 8:45 PM To: histonet Subject: [Histonet] NSH Meeting Hotels Hi All, Anyone trying to reserve a room at the Doubletree should read this. I just tried to reserve a room for the upcoming Symposium. I went to the NSH website and looked under the Hotel Travel icon and saw that the only hotel that wasn't Sold Out was the Double Tree. I called and they never heard of us NSH. I read all the information to the reservation person and she came up with a blank. I requested the supervisor. After searching for about 15 minutes, she found us under histotechnology. Anyone trying to book a room should state that our GROUP CODE is CI0N Akemi Allison-Tacha BS, HT(ASCP)HTL Histology Manager APMG Laboratories 105A Cooper Court, Los Gatos, CA 95032 Contact: 800.848.2764 V/M: 408.884.2718 Fax: 408.884.2758 Cell: 408.335.9994 (W) E-Mail: aallison-ta...@apmglab.com (P) E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] General Orientation Checklist
I am also interested. Could someone please send it to me? :) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Friday, July 31, 2009 7:45 AM To: Amy Self; tahs...@brain.net.pk; Cristi stephenson Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] General Orientation Checklist I too am interested in the information. Please send it to me. I tried to send information with an attachment in the past to histonet and it never went through. I guess the only way an attachment can be sent is to an individual. Links are acceptable. Regards,Akemi Akemi Allison-Tacha, BS, HT/HTLE-Mail: akemiat3...@yahoo.com --- On Thu, 7/30/09, Cristi stephenson cls71...@sbcglobal.net wrote: From: Cristi stephenson cls71...@sbcglobal.net Subject: Re: [Histonet] General Orientation Checklist To: Amy Self as...@georgetownhospitalsystem.org, tahs...@brain.net.pk Cc: histonet@lists.utsouthwestern.edu Date: Thursday, July 30, 2009, 8:46 PM Hello, I am sorry, I am also interested in this, but I don't see the attachment? Thanks, Cristi Stephenson MSA, HT(ASCP) --- On Wed, 7/29/09, tahs...@brain.net.pk tahs...@brain.net.pk wrote: From: tahs...@brain.net.pk tahs...@brain.net.pk Subject: Re: [Histonet] General Orientation Checklist To: Amy Self as...@georgetownhospitalsystem.org Cc: histonet@lists.utsouthwestern.edu Date: Wednesday, July 29, 2009, 8:08 AM The file is attached with. M. Tahseen, Supervisor Histopathology SKMCHRC, Lahore, Pakistan Good Day Histonetters, Does anyone have a general orientation checklist for histology for new hires that they would be willing to share with me? Thanks in advance for your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -Inline Attachment Follows- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Muscle Artifact
This number did not work for me at the Fisher website. Is it current? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barone, Carol Sent: Wednesday, July 29, 2009 2:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Muscle Artifact Containers: Fisher # NC9283918 $32.90/6. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, July 29, 2009 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 68, Issue 38 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Artifact in Muscle bx's frozen for extended time (Sharon Allen) 2. flash frozen tissue (Kalleberg, Kristopher) 3. Mouse Eyes (Jo-Ann Bader, Ms.) 4. SLIDE PRINTER (Janice Mitchell) 5. Re: Mouse Eyes (Rene J Buesa) -- Message: 1 Date: Wed, 29 Jul 2009 09:27:24 -0500 From: Sharon Allen sal...@exchange.hsc.mb.ca Subject: [Histonet] Artifact in Muscle bx's frozen for extended time To: histo...@pathology.swmed.edu Message-ID: bb6adcd4b7abb045a09a7634ac15cc610bec8...@hscxmsmx0010.ad.wrha.mb.ca Content-Type: text/plain; charset=iso-8859-1 Hi, I would like suggestions on how to eliminate or decrease the effects of extended storage of muscle bx's in a -70°C freezer. We do approx. 150 muscle bx's /year in our Neuropathology Lab and we keep all frozen muscle bx's indefinitely, (we have them all from the 80's on). We are asked fairly regularly to go back to these cases for diagnostic research purposes find many of them are compromised, readable but not optimum, I am assuming from drying out from months/years of freezing. We freeze them using the isopentane/liquid nitrogen method on a pc of cork with OCT anchoring them. We store them in a small plastic tube with a screw top. I would like to know if anyone has further methods to eliminate this artefact. Possibly sealing with OCT? Placing water in the bottom of the tube? Wrapping the muscle bx in Parafilm, tin foil etc? What containers do you use to store them in if you do not get this artefact? Suggestions for long term storage would be very much appreciated. Thanks Sharon Allen Senior Neuropathology Technologist HSC WPG, MB, CA This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destiné à la personne ou aux personnes à qui il est adressé. Il peut contenir des informations privilégiées ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autorisée est strictement défendue. Si vous n'êtes pas le destinataire de ce message, veuillez en informer l'expéditeur immédiatement et lui remettre l'original. -- Message: 2 Date: Wed, 29 Jul 2009 10:31:39 -0400 From: Kalleberg, Kristopher kristopher.kalleb...@unilever.com Subject: [Histonet] flash frozen tissue To: histonet@lists.utsouthwestern.edu Message-ID: 0e6bc087f70f9c47acff2c203d6e329c067f7...@ntrsevs30002.s3.ms.unilever.com Content-Type: text/plain; charset=us-ascii Hello All, I will be running a study upcoming and we have decided not to run FFPE as we normally do and have decided to use flash frozen. My big concern is what is the proper way to fix all of these SKIN samples to use for IHC. Any recommendations as to if prefix the samples in sucrose and then flash freeze in OCT is best or any recommendations as to if post fixing (in acetone??) the already sectioned, flash frozen sample in OCT is best. Any and and all help will be greatly appreciated. Thank you. Kris Kalleberg -- Message: 3 Date: Wed, 29 Jul 2009 11:38:05 -0400 From: Jo-Ann Bader, Ms. jo-ann.ba...@mcgill.ca Subject: [Histonet] Mouse Eyes To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 76d119ef12c904418800ed67ccb2062905e2ab9...@exmbxvs1.campus.mcgill.ca Content-Type: text/plain;
[Histonet] brilliant blue safranin O
Has anyone done this stain for chromatin and spindles in cell culture? My results in the search for this technique are very sketchy. Katherine Walters Histology Director Central Microscopy Research Facilities 85 Eckstein Medical Research Building University of Iowa Iowa City, Iowa 52242-1101 katherine-walt...@uiowa.edu www.uiowa.edu/~cemrf ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] BRDU, tetracycline and calcein IV in bone
This may be a question for Gayle, or someone else who specializes in bone. I will be doing a project involving the facial bones of 8 week-old rabbits. We want to look at regeneration of bone in this area. I have some experience with BRDU in mouse tissues. My questions are: 1.) Will decalcification affect the BRDU signal? If not, what is the best method for decalcification? Approximately how long? 2.) Do I need to use a ground section for tetracycline and calcein IV or can I decalcify and paraffin embed? 3.) What is the best fixation procedure? 4.) Anything I need to know, but am too naive to ask? Thanks for any information you can share! Katherine Walters Histology Director Central Microscopy Research Facilities 85 Eckstein Medical Research Building University of Iowa Iowa City, Iowa 52242-1101 katherine-walt...@uiowa.edu www.uiowa.edu/~cemrf Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Masson's trichrome stain
I am having a problem with Biebrich Scarlet. I am attempting to do a Masson's stain on some mouse heart tissue. Instead of the strikingly bright red of the muscle tissue I am getting a purple color instead. Everything else looks fine. The hearts are fixed in Penfix and are embedded in paraffin. We make the solution up fresh for each run. I am using Sigma's Ponceau BS (CI 26905). Any ideas? Katherine Walters Histology Director Central Microscopy Research Facilities 85 Eckstein Medical Research Building University of Iowa Iowa City, Iowa 52242-1101 katherine-walt...@uiowa.edu www.uiowa.edu/~cemrf Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cresyl violet problems
To the brainy histo-people, I have a student doing a cresyl violet stain on some frozen (40um) brain sections. He has been doing this routinely for a couple of months, but his last run looks very odd. There appears to be an area on each section where there is no staining. At first glance I thought it was a fixation problem, but the unstained portion of the tissue is in a different area as you move from slice to slice (these are serial sections), He stains in a Copeland jar and assures me that his stain is well homogenized. Could this be some kind of moisture problem? Thanks for your thoughts. Katherine Walters Histology Director Central Microscopy Research Facilities 85 Eckstein Medical Research Building University of Iowa Iowa City, Iowa 52242-1101 katherine-walt...@uiowa.edu www.uiowa.edu/~cemrf Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (reply) silly questions.---PFA
This may be another silly question, but how does one test the concentration of formaldehyde in solution? Thanks, Kathy Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of tf Sent: Friday, December 12, 2008 2:35 AM To: Tony Henwood; Pat Flannery; histo...@lists.utsouthwestern.ed Subject: [Histonet] (reply) silly questions.---PFA I looked at the sections and the cell shrinkage (and prominent spaces between cells and connective tissue) indicated that most of the fixation seemed to have occured in the processing ethanols. I asked him for some of the fixative he used, tested the formaldehyde concentration and found it to be less than 0.5%!! Tony: Do you think this is because of inproper preparation of PFA in his lab, or the common problem in all researchers using PFA? I do think most biomedical labs currently are using PFA to prepare the fixatives! So, anyone has the idea on a correction preparation procedure of 4% PFA? I noticed some of you dissolve PFA powder in NaOH-conditioned alkaline water, then add concentrated PB solution. We here dissolve PFA in concentrated PB solution directly (heat stir for 2-3 hours), then adjust pH to 7.4. We dont have big problem in tissue quailityexcept when one want to cut the brain in a cryostat rather sliding microtome. Many times the brain sections from the cryostat have cheese like holes/cavities, which almost never appear on sliding microtome-prepared sections. 2008-12-12 tf 发件人： Tony Henwood 发送时间： 2008-12-12 06:18:47 收件人： Pat Flannery; histonet@lists.utsouthwestern.edu 抄送： 主题： RE: [Histonet] Silly Question? Pat, I agree with you. In a routine diagnostic histopathology laboratory, it makes little difference what you use. Around the world for over 100 years most labs use 10% neutral buffered formalin made from concentrated 38%(or there abouts) formalin (or formaldehyde). Researchers, though, are a different kettle of fish. They will tend to hang on to misinformed, mystical methods believing they are being scientific. Funny, you would think that they, as a group, would be the ones pushing the boundaries and critically assessing each step of their research, ensuring that they understand what and why they are doing it. (Disclaimer - not all researchers are like this, thank heavens!!) Using a formaldehyde solution made from polyformaldehyde can cause problems. One researcher used it and wondered why their morphology was sub-optimal and their p53 immunohistochemistry was negative. He assured me that he had fixed small samples of tissue for 6 hours in 4% formaldehyde and then processed them using ethanol, xylene and wax. I looked at the sections and the cell shrinkage (and prominent spaces between cells and connective tissue) indicated that most of the fixation seemed to have occured in the processing ethanols. I asked him for some of the fixative he used, tested the formaldehyde concentration and found it to be less than 0.5%!! This also explains the loss of p53 staining. I gave him some of our routine 10% phosphate buffered fomalin, asked him to fix overnight, and try agin. Low and behold problem solved. How's that for a Friday Flamming!!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pat Flannery Sent: Friday, 12 December 2008 3:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Silly Question? Please humor me on this if it's obvious (to everyone but me): why do we use paraformaldehyde (which is so inconvenient to make up) rather than buffered formalin or just diluted formaldehyde itself? It seems that around here, some folks prefer paraformaldehyde (either 2% or 4%) and others use formalin, while some others stick to diluted formaldehyde (I see all 4 on labels for specimens submitted for histology). Is it mostly a matter of personal preference or where you were trained (i.e. force of habit) or is there a valid reason to use each solution (basically the same chemical once in solution, merely buffered or not)? The only answer I've gotten when I've asked is, That's what we always use. Thanks. -Pat Flannery (not a real
RE: [Histonet] Re: bluing hematoxylin and alkaline water????
This is why I love the histonet!! -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Robert Richmond Sent: Friday, November 21, 2008 2:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: bluing hematoxylin and alkaline water Hard water (water containing dissolved calcium carbonate and/or sulfate is alkaline enough to use as a bluing agent by itself. New York City, Hot Springs Arkansas, and San Antonio Texas - in my personal experience - have tap water sufficiently alkaline that you can blue hematoxylin in it in a reasonable length of time. The original Scott's solution was in fact devised as a substitute for alkaline tap water. Scott SG (Oxford). On successive double staining for histological purposes-preliminary note. Journal of Pathology and Bacteriology 1911-1912: 16,390-8. Scott notes that As tap water varies in constitution from place to place, and even the alkaline tap water of Oxford from the oölite Cotswolds requires ten minutes with occasional changes for safe removal of acid, the artificial substitute mentioned above has been introduced. Robert Wyllie in 1970 told me that this bluing solution was widely used in Australia, apparently introduced by Oxonian histologists nostalgic for the tap water of their homeland. Wyllie was an Australian histochemist who worked in the pathology department at Johns Hopkins for a number of years. He greatly simplified Scott's original formula, and referred to this preparation as Scott's solution. Gary Gill popularized Scott's solution along with his well-known hematoxylins. Bob Wyllie (of blest memory) was an absolute genius in the histochemistry lab, but he was a very modest man. He published very little, and many of his ingenious techniques are totally lost (I have just a few of them). I spent a year as a research fellow in his laboratory in 1970. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
