[Histonet] H Stainers

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Is there a stainer other than Ventana's that has all the reagents in prefilled 
bottles?Rae Ann Staskiewicz HT(ASCP)Sent from my Verizon, Samsung Galaxy 
smartphone
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[Histonet] Blades

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What breand of blades do people prefer??Rae Staskiewicz UnityPoint Health 
Methodist PeoriaSent from my Verizon, Samsung Galaxy smartphone
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Re: [Histonet] Recommended thickness of Amyloid sections

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Should also be cut on the day they are to be stained.Rae Staskiewicz 
HT(ASCP)Sent from my Verizon, Samsung Galaxy smartphone
 Original message From: Paula Keene Pierce via Histonet 
 Date: 4/16/20  3:24 PM  (GMT-06:00) To: 
histonet@lists.utsouthwestern.edu, Ken M  Subject: Re: 
[Histonet] Recommended thickness of Amyloid sections As this is my 41 year of 
being a registered HT, I was taught that slides for amyloid are to be cut at 
8-10µm.Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, 
Inc.5830 N Blue Lake DriveNorman, OK 73069PH 
405-759-3953http://www.excaliburpathology.comA sharp knife is nothing without a 
sharp eye. - Klingon Proverb     On Thursday, April 16, 2020, 03:18:44 PM CDT, 
Ken M via Histonet  wrote:    Hello All.  We 
have always cut all of our histology control slides at 5m.  We were told today 
that it is common practice to cut Amyloid at 8m?  Is this your 
experience?Ken___Histonet mailing 
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Re: [Histonet] Microtome at home

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I wouldn't think there would be any problem.Rae Staskiewicz HT(ASCP)Sent from 
my Verizon, Samsung Galaxy smartphone
 Original message From: Jamie Watson via Histonet 
 Date: 4/15/20  6:44 PM  (GMT-06:00) To: 
Histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome at home Hello 
all,Our pathologist has come up with the idea of sending a microtome and 
waterbath home to someone that cannot come to work due to COVID 19.  We are a 
research lab and work with mouse and rat tissue.  Does anyone know of any 
issues with doing this?  I have never heard of anyone cutting slides at home 
other than someone with a private business.Thank 
you.Jamie___Histonet mailing 
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[Histonet] Animal and Human Tissues

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Is anyone processing both animal and human  tissues? If so my Lab Director 
would like to talk with you.Rae Ann Staskiewicz HT(ASCP)UnityPoint Methodist 
Peoria 309-672-4930Sent from my Verizon, Samsung Galaxy smartphone
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[Histonet] Joint Commission

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Has anyone heard of a regulation by the Joint Commission to require grossing be 
done in a negative pressure room?
Rae StaskiewiczUnity point Health MethodistPeoria, Il

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[Histonet] Short biopsy run

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Does anyone have a short biopsy protocol for a VIP 6   or VIP 6 AI? If so could 
you please share it with us? 

Thank you in advance. 

Rae Staskiewicz 
UnityPoint  health 
Methodist, Peoria IL 
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[Histonet] Control block for asbestos

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Does anyone have or know where to obtain control slides for Perl's Iron stain 
for asbestos? 

Thanks in advance, 
Rae A Staskiewicz HT (ASCP) 
UnityPoint Health| Methodist|Proctor 
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[Histonet] Antibody MyD88

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Does anyone have a protocol using the Ventana Ultra for this antibody? Thanks 
in advance. 

Rae Staskiewicz 
UnityPoint Health-Methodist/Proctor 
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[Histonet] ALK-1

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Does anyone out there have a protocol for ALK-1, using the Benchmark Ultra and 
Ventana Ultraview detection kit? If so I would greatly appreciate it. Thanks in 
advance. 

Rae Staskiewicz 
Unitypoint Health-Methodist Peoria 

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[Histonet] Acid hematin

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I saw a Histonet post that Peggy Wenk had made concerning the recipe for 
alcoholic formalin for processing. She noted that you use the solution 
unbuffered.  How do you control acid hematin pigment in such a solution? 
We use a commercial alcoholic formalin “concentrate” that is diluted with 
alcohol and water prior to use.  It is supposedly buffered.  We have great 
problems with acid hematin.  Do you have any suggestions?  For preprocessing 
fixation, we make our own buffered formalin from scratch or from a concentrate 
and get biopsy specimens from veterinarians fixed in who-knows-what. 
Thank you very much, 
DW 
  
Card
  

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