Re: [Histonet] Active Caspase-3 IHC-P staining
Have a look at results in Histonet images? Good luck Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6810 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Active Caspase-3 IHC-P staining
If you have access to tissues, rather than purchasing cell lines, you can use spleen, gut, lymph nodes. They will all have a lot of apoptosis. You can use a lot of different tissues for negative controls: I prefer brain or muscle since they are less dense with nuclei. Val > On Jun 23, 2022, at 10:32 AM, Charles Riley via Histonet > wrote: > > I am currently running a research project and the researcher wants me to > use Caspase-3 for apoptosis staining. I have never worked with this > antibody before and want to know if anyone has any suggestions on what to > use for a positive tissue control and a negative tissue control? > > > I purchased the antibody from Novus Biologicals and they recommend a Jurkat > cell lysate for positive control but I am not familiar with this as well. > > Any guidance would be greatly appreciated > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Active Caspase-3 IHC-P staining
What species are you staining?? Amy S. Porter, HT (ASCP) - Lab Supervisor Michigan State University Departments of Physiology & Pathology Investigative HistoPathology Lab 567 Wilson Road - Rm 2133 East Lansing, MI 48824 Ph: 517-884-5026 (she/her/hers) https://sites.google.com/msu.edu/ihpl/home "Life is like riding a bicycle. To keep your balance, you must keep moving." - Albert Einstein -Original Message- From: Charles Riley via Histonet Sent: Thursday, June 23, 2022 1:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Active Caspase-3 IHC-P staining I am currently running a research project and the researcher wants me to use Caspase-3 for apoptosis staining. I have never worked with this antibody before and want to know if anyone has any suggestions on what to use for a positive tissue control and a negative tissue control? I purchased the antibody from Novus Biologicals and they recommend a Jurkat cell lysate for positive control but I am not familiar with this as well. Any guidance would be greatly appreciated ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!xyBsRdDpZSchS-atSTL78qekJGvTEx9dKu9K1y7IVA7l5o-LPaWNWLRv2PASuqtgcrm-OQLBRf04blsJq4zdxucLZzY39A$ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Active Caspase-3 IHC-P staining
I am currently running a research project and the researcher wants me to use Caspase-3 for apoptosis staining. I have never worked with this antibody before and want to know if anyone has any suggestions on what to use for a positive tissue control and a negative tissue control? I purchased the antibody from Novus Biologicals and they recommend a Jurkat cell lysate for positive control but I am not familiar with this as well. Any guidance would be greatly appreciated ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
