[Histonet] CAP and negative IHC controls
How is everyone interpreting the CAP rule about using negative controls? Do we still need a negative with each patient slide? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CAP and negative IHC controls
It is my understanding that you still need a negative tissue control but if using a polymer detection kit that you may stop doing a negative reagent control. The negative tissue control is a tissue known to not contain the antigen in the tissue, it can be an internal control, or a separate control but if internal control, then it must be designated as to how it will be used in your procedure manual. Hope that helps. Debbie Siena 800.442.3573 ext. 229 | www.statlab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jsjurc...@comcast.net Sent: Friday, December 28, 2012 2:19 PM To: . Subject: [Histonet] CAP and negative IHC controls How is everyone interpreting the CAP rule about using negative controls? Do we still need a negative with each patient slide? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CAP and negative IHC controls
That decision needs to be made by your Medical Director. In my laboratory we use polymer detection for almost all of our IHCs and, therefore, I don't require a Negative Reagent Control for those cases. We have one antibody that requires avidin-biotin detection and we run a negative reagent control in parallel whenever we run that antibody. In my experience, if you are using polymer detection and your antibodies are properly optimized and validated, the negative reagent control slide serves no useful purpose and, therefore, is not needed. Best wishes to everyone on Histonet for the coming New Year! Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax jsjurc...@comcast.net 12/28/2012 3:18 PM How is everyone interpreting the CAP rule about using negative controls? Do we still need a negative with each patient slide? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
