[Histonet] Controls
Hi fellow Histo people! I am setting up a new histology lab and I am needing to find inventive ways to obtain controls for IHC and Special stains. I have lots of normal tissue but I am in need of melanoma, P16 positive, fungus, positive p63, and positive p504. If anyone would be interested in helping me out I would greatly appreciate it. Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Controls
I would recommend waiting at least 2 weeks after the case is signed-out in case additional testing needs to be performed, especially if the specimen contains cancer. And, I would always make sure that you leave representative tissue in the block meaning don't consume the entire specimen when you cut your control sections unless the case has multiple blocks showing the same pathology. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) richard.car...@hhchealth.org -Original Message- From: Jacque Sagasser via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, October 15, 2020 12:47 PM To: 'Histonet (histonet@lists.utsouthwestern.edu)' Subject: [Histonet] Controls STOP, THINK, READ. This is an external email. Exercise extra caution responding to it, opening attachments and following links. Good afternoon! Could any of you tell me off hand how old patient tissue has to be before you can use it as a control? My lab has only been operating since February. We run quite a few Alcian Blue PAS stains a week, and my last control is getting sparse. I have some beautiful patient tissue that I could use if the time limit has been exceeded. I am trying to avoid purchasing controls because we are a small GI lab, and that eats into our operating costs. That said, if tissue has to be older than the amount of time our lab has been open, does anyone have a spare control they could pass on to me please? I would be willing to assist you with anything I am able to assist with in the future. Jacque R. Sagasser, HT (ASCP)cm Gandhi GI Pathology, LLC 999 Brubaker Drive Suite 1 Kettering OH 45429 jsagas...@gandhigi.com 937-795-1099 phone 937-519-1321 fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KCs9X-8!JE_DSMiJJOIqcoBfBHt09qvO0We4-zpHEADQfbNIr282bdD0Xju5hESmd9xhVlW2bMo$ Reminder: This e-mail and any attachments are subject to the current HHC email retention policies. Please save or store appropriately in accordance with policy. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Controls
Good afternoon! Could any of you tell me off hand how old patient tissue has to be before you can use it as a control? My lab has only been operating since February. We run quite a few Alcian Blue PAS stains a week, and my last control is getting sparse. I have some beautiful patient tissue that I could use if the time limit has been exceeded. I am trying to avoid purchasing controls because we are a small GI lab, and that eats into our operating costs. That said, if tissue has to be older than the amount of time our lab has been open, does anyone have a spare control they could pass on to me please? I would be willing to assist you with anything I am able to assist with in the future. Jacque R. Sagasser, HT (ASCP)cm Gandhi GI Pathology, LLC 999 Brubaker Drive Suite 1 Kettering OH 45429 jsagas...@gandhigi.com 937-795-1099 phone 937-519-1321 fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] controls to lengthy off topic
Hello Garrey, Curious myself, CAP contact info seems to be greyed out on website unless I officially log in and for now my concerns are with the Washington State Science and Engineering Fair for K-12 and golf game. (1) There are at least two phrases in the ANP.21450 which could be parsed out similar to the now famous it depends on what the definition of is is. (2) Fortunate, I had micro groups around who could provide me with species specific Candida or Aspergillus or species and morphological identifiable gram positive or gram negative organisms so when I built the controls with fresh human tissue, as has been described several times on Histonet by others, I knew exactly what I was looking at. (3) It appears there may be tens to hundreds of thousands of molds and what is growing in orange peels or strawberries or cream cheese or bacteria in slim jims would be a total mystery but maybe that is OK. Yet, human pathogen or not? rare or common? stains appropriately or not according to what it REALLY is? I'm not saying the controls are wrong; they might be perfectly fine. I'm just curious if anyone being inspected ever put a stained section of a slim jim on scope in front of a Pathologist from the inspecting agency and what was the reaction if any. Ray in Lake Forest Park, WA - Original Message - From: Garrey Faller garr...@gmail.com To: koelli...@comcast.net Cc: tjfinney2...@gmail.com, histonet@lists.utsouthwestern.edu Sent: Monday, April 20, 2015 3:50:15 PM Subject: Re: [Histonet] (no subject) Here is the CAP checklist requirement: ANP.21450 All histochemical stains are of adequate quality, and daily controls are demonstrated on each day of use for the tissue components or organism for which they were designed. Ray...you should call the CAP and ask for guidance on this. My interpretation of this requirement is that it should be OK to use a fungus from an orange peel. An orange peel fungus should have the same staining characteristics as a candida or aspergillus etc. Similarly a bacteria is a bacteria. If you can produce a control that has both gram positives and negatives, it should be OK. But, don't quote me on this. Call the CAP for a definitive answer. I am interested in their response. Garrey On Sun, Apr 19, 2015 at 9:06 PM, koelli...@comcast.net wrote: I asked about this in a different vein months ago. Has anyone shown a strawberry or ground meat or slim jim or orange peel as a bacteria/fungus control used for diagnostics to an inspector inspecting the lab and was there any comment from the inspector either positive or negative. Never heard back anything. Ray, Lake Forest Park, WA - Original Message - From: tjfinney2...@gmail.com To: histonet@lists.utsouthwestern.edu Sent: Sunday, April 19, 2015 5:24:53 PM Subject: [Histonet] (no subject) GMS controls From my understanding we can't use non human controls on patients. I could be wrong, but you may want to look into it. Happy Connecting. Sent from my Sprint Phone. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: Re[2]: [Histonet] Controls
Ok, hamburger is good for what control? Paula Sent from my iPhone On Mar 5, 2015, at 10:01 AM, Brendal Finlay brendal.fin...@medicalcenterclinic.com wrote: Ok, I'm curious. Would any of these work for an AFB control? Brendal C. Finlay, HT (ASCP) Senior Histologist Medical Center Clinic 8333 North Davis Highway Pensacola, FL 32514 Phone 850.474.8581 Fax 850.474.8584 -Original Message- From: Garreyf garr...@gmail.com To: Ronda Mire rm...@cvpath.org Cc: histonet@lists.utsouthwestern.edu, Baker, Michael michael.ba...@cchmc.org Date: 03/05/2015 11:10 Subject: Re: [Histonet] Controls I am in need of both a gram and fungal control and will have to try the slim Jim, hamburger and hotdog tricks. Thanks. If they work I will post A link to pictures. Sent from my iPhone On Mar 5, 2015, at 11:58 AM, Ronda Mire rm...@cvpath.org wrote: Slim Jim will work as a control for gram stain. Can you believe people eat this crap? On Mar 5, 2015, at 11:40 AM, Baker, Michael michael.ba...@cchmc.org wrote: Sounds like a plant spreading anti-Slim Jim propaganda. What’s next? Donut holes? Michael Baker, M.D. CCHMC Pathology On Mar 5, 2015, at 10:51 AM, histonet-requ...@lists.utsouthwestern.edu wrote: -- Message: 9 Date: Wed, 4 Mar 2015 14:04:52 -0700 From: Jb craiga...@gmail.com Subject: [Histonet] Controls: To: Histonet@lists.utsouthwestern.edu Message-ID: b98f49d1-de91-4675-b53b-a34cfcc43...@gmail.com Content-Type: text/plain;charset=us-ascii Off the wall question, I have been told that slim jims (pepperoni stick) at the gas station can be processed and used as good gram controls. Has anyone done this and do they work for GMS also? Thank you, Sent from my iPhone -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Controls
I agree it sounds bad, But the reality is that all food products have loads of dead or low count living bacteria. For example, milk is pasteurized to kill them, but they are still in the milk. Tim Morken -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ronda Mire Sent: Thursday, March 05, 2015 8:59 AM To: Baker, Michael Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Controls Slim Jim will work as a control for gram stain. Can you believe people eat this crap? On Mar 5, 2015, at 11:40 AM, Baker, Michael michael.ba...@cchmc.org wrote: Sounds like a plant spreading anti-Slim Jim propaganda. What’s next? Donut holes? Michael Baker, M.D. CCHMC Pathology On Mar 5, 2015, at 10:51 AM, histonet-requ...@lists.utsouthwestern.edu wrote: -- Message: 9 Date: Wed, 4 Mar 2015 14:04:52 -0700 From: Jb craiga...@gmail.com Subject: [Histonet] Controls: To: Histonet@lists.utsouthwestern.edu Message-ID: b98f49d1-de91-4675-b53b-a34cfcc43...@gmail.com Content-Type: text/plain;charset=us-ascii Off the wall question, I have been told that slim jims (pepperoni stick) at the gas station can be processed and used as good gram controls. Has anyone done this and do they work for GMS also? Thank you, Sent from my iPhone -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Controls:
We have successfully used hamburger meat to make Gram controls. As far as GMS controls go, we ran across a post from someone smearing cream cheese onto lung tissue and letting it sit for a couple of days, fixed and processed it and were able to demonstrate Aspergillus by GMS. My fungus control stocks are low so I was actually planning to try this with some beef lung. I haven't heard of the Slim Jim method before. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Wednesday, March 04, 2015 1:05 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Controls: Off the wall question, I have been told that slim jims (pepperoni stick) at the gas station can be processed and used as good gram controls. Has anyone done this and do they work for GMS also? Thank you, Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Controls
I am in need of both a gram and fungal control and will have to try the slim Jim, hamburger and hotdog tricks. Thanks. If they work I will post A link to pictures. Sent from my iPhone On Mar 5, 2015, at 11:58 AM, Ronda Mire rm...@cvpath.org wrote: Slim Jim will work as a control for gram stain. Can you believe people eat this crap? On Mar 5, 2015, at 11:40 AM, Baker, Michael michael.ba...@cchmc.org wrote: Sounds like a plant spreading anti-Slim Jim propaganda. What’s next? Donut holes? Michael Baker, M.D. CCHMC Pathology On Mar 5, 2015, at 10:51 AM, histonet-requ...@lists.utsouthwestern.edu wrote: -- Message: 9 Date: Wed, 4 Mar 2015 14:04:52 -0700 From: Jb craiga...@gmail.com Subject: [Histonet] Controls: To: Histonet@lists.utsouthwestern.edu Message-ID: b98f49d1-de91-4675-b53b-a34cfcc43...@gmail.com Content-Type: text/plain;charset=us-ascii Off the wall question, I have been told that slim jims (pepperoni stick) at the gas station can be processed and used as good gram controls. Has anyone done this and do they work for GMS also? Thank you, Sent from my iPhone -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Controls:
Slim Jims do work for use as Gram Controls -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Thursday, March 05, 2015 10:44 AM To: Jeffrey Robinson; Jb; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls: There was actually an article written in The Journal of Histotechnology a few years back on this and it was, I f I recall, using a hot dog to create the gram control. Might be in the archives on nsh.org. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson Sent: Thursday, March 05, 2015 9:21 AM To: Jb; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls: We have successfully used hamburger meat to make Gram controls. As far as GMS controls go, we ran across a post from someone smearing cream cheese onto lung tissue and letting it sit for a couple of days, fixed and processed it and were able to demonstrate Aspergillus by GMS. My fungus control stocks are low so I was actually planning to try this with some beef lung. I haven't heard of the Slim Jim method before. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Wednesday, March 04, 2015 1:05 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Controls: Off the wall question, I have been told that slim jims (pepperoni stick) at the gas station can be processed and used as good gram controls. Has anyone done this and do they work for GMS also? Thank you, Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Controls
Slim Jim will work as a control for gram stain. Can you believe people eat this crap? On Mar 5, 2015, at 11:40 AM, Baker, Michael michael.ba...@cchmc.org wrote: Sounds like a plant spreading anti-Slim Jim propaganda. What’s next? Donut holes? Michael Baker, M.D. CCHMC Pathology On Mar 5, 2015, at 10:51 AM, histonet-requ...@lists.utsouthwestern.edu wrote: -- Message: 9 Date: Wed, 4 Mar 2015 14:04:52 -0700 From: Jb craiga...@gmail.com Subject: [Histonet] Controls: To: Histonet@lists.utsouthwestern.edu Message-ID: b98f49d1-de91-4675-b53b-a34cfcc43...@gmail.com Content-Type: text/plain;charset=us-ascii Off the wall question, I have been told that slim jims (pepperoni stick) at the gas station can be processed and used as good gram controls. Has anyone done this and do they work for GMS also? Thank you, Sent from my iPhone -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Controls:
There was actually an article written in The Journal of Histotechnology a few years back on this and it was, I f I recall, using a hot dog to create the gram control. Might be in the archives on nsh.org. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson Sent: Thursday, March 05, 2015 9:21 AM To: Jb; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls: We have successfully used hamburger meat to make Gram controls. As far as GMS controls go, we ran across a post from someone smearing cream cheese onto lung tissue and letting it sit for a couple of days, fixed and processed it and were able to demonstrate Aspergillus by GMS. My fungus control stocks are low so I was actually planning to try this with some beef lung. I haven't heard of the Slim Jim method before. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Wednesday, March 04, 2015 1:05 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Controls: Off the wall question, I have been told that slim jims (pepperoni stick) at the gas station can be processed and used as good gram controls. Has anyone done this and do they work for GMS also? Thank you, Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Controls:
Years ago (70's and 80's) Slim Jims were used as Gram pos neg controls in all the labs at AFIP. James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel 858-332-4647 Fax 858-812-1915 jwat...@gnf.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Wednesday, March 04, 2015 1:05 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Controls: Off the wall question, I have been told that slim jims (pepperoni stick) at the gas station can be processed and used as good gram controls. Has anyone done this and do they work for GMS also? Thank you, Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Controls:
I've used moldy orange peels as a GMS/PAS-F control in the past. Just saved the peels in a plastic bag over the weekend, then processed as usual. They worked beautifully! Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 Pager: 213-209-0184 bcoo...@chla.usc.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson Sent: Thursday, March 05, 2015 7:21 AM To: Jb; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls: We have successfully used hamburger meat to make Gram controls. As far as GMS controls go, we ran across a post from someone smearing cream cheese onto lung tissue and letting it sit for a couple of days, fixed and processed it and were able to demonstrate Aspergillus by GMS. My fungus control stocks are low so I was actually planning to try this with some beef lung. I haven't heard of the Slim Jim method before. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Wednesday, March 04, 2015 1:05 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Controls: Off the wall question, I have been told that slim jims (pepperoni stick) at the gas station can be processed and used as good gram controls. Has anyone done this and do they work for GMS also? Thank you, Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Controls:
So do onions! On Thu, Mar 5, 2015 at 12:06 PM, Cooper, Brian bcoo...@chla.usc.edu wrote: I've used moldy orange peels as a GMS/PAS-F control in the past. Just saved the peels in a plastic bag over the weekend, then processed as usual. They worked beautifully! Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 Pager: 213-209-0184 bcoo...@chla.usc.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson Sent: Thursday, March 05, 2015 7:21 AM To: Jb; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls: We have successfully used hamburger meat to make Gram controls. As far as GMS controls go, we ran across a post from someone smearing cream cheese onto lung tissue and letting it sit for a couple of days, fixed and processed it and were able to demonstrate Aspergillus by GMS. My fungus control stocks are low so I was actually planning to try this with some beef lung. I haven't heard of the Slim Jim method before. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Wednesday, March 04, 2015 1:05 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Controls: Off the wall question, I have been told that slim jims (pepperoni stick) at the gas station can be processed and used as good gram controls. Has anyone done this and do they work for GMS also? Thank you, Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jim Burchette Fly Fishing Bum *'(((* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re[2]: [Histonet] Controls
Ok, I'm curious. Would any of these work for an AFB control? Brendal C. Finlay, HT (ASCP) Senior Histologist Medical Center Clinic 8333 North Davis Highway Pensacola, FL 32514 Phone 850.474.8581 Fax 850.474.8584 -Original Message- From: Garreyf garr...@gmail.com To: Ronda Mire rm...@cvpath.org Cc: histonet@lists.utsouthwestern.edu, Baker, Michael michael.ba...@cchmc.org Date: 03/05/2015 11:10 Subject: Re: [Histonet] Controls I am in need of both a gram and fungal control and will have to try the slim Jim, hamburger and hotdog tricks. Thanks. If they work I will post A link to pictures. Sent from my iPhone On Mar 5, 2015, at 11:58 AM, Ronda Mire rm...@cvpath.org wrote: Slim Jim will work as a control for gram stain. Can you believe people eat this crap? On Mar 5, 2015, at 11:40 AM, Baker, Michael michael.ba...@cchmc.org wrote: Sounds like a plant spreading anti-Slim Jim propaganda. What’s next? Donut holes? Michael Baker, M.D. CCHMC Pathology On Mar 5, 2015, at 10:51 AM, histonet-requ...@lists.utsouthwestern.edu wrote: -- Message: 9 Date: Wed, 4 Mar 2015 14:04:52 -0700 From: Jb craiga...@gmail.com Subject: [Histonet] Controls: To: Histonet@lists.utsouthwestern.edu Message-ID: b98f49d1-de91-4675-b53b-a34cfcc43...@gmail.com Content-Type: text/plain; charset=us-ascii Off the wall question, I have been told that slim jims (pepperoni stick) at the gas station can be processed and used as good gram controls. Has anyone done this and do they work for GMS also? Thank you, Sent from my iPhone -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Controls:
Do you mean using Slim Jims right out of the package, as is, for Gram controls? What is in those things - sounds like a food safety issue! -Nancy Stedman -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Thursday, March 05, 2015 1:01 PM To: 'Jb'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls: Years ago (70's and 80's) Slim Jims were used as Gram pos neg controls in all the labs at AFIP. James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel 858-332-4647 Fax 858-812-1915 jwat...@gnf.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Wednesday, March 04, 2015 1:05 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Controls: Off the wall question, I have been told that slim jims (pepperoni stick) at the gas station can be processed and used as good gram controls. Has anyone done this and do they work for GMS also? Thank you, Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Controls:
Off the wall question, I have been told that slim jims (pepperoni stick) at the gas station can be processed and used as good gram controls. Has anyone done this and do they work for GMS also? Thank you, Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Controls
Help! Running low on CMV and AFB positive control blocks. Looking into buying precut slides but the price is steep. Any suggestion ? Which company has the best price. The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] controls
Is a candid control one that's taken when the fungus doesn't know you're sampling it? Sincerely? Jay A Lundgren, M.S., (HTL) ASCP On Fri, Jan 10, 2014 at 11:58 AM, Webb, Dorothy L dorothy.l.w...@healthpartners.com wrote: Does anyone out in histoland have a Candid control for fungus that you could spare? We are very much in need and would appreciate the help and see what we could possibly trade for. Much thanks and have a great week-end all! Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] controls
Does anyone out in histoland have a Candid control for fungus that you could spare? We are very much in need and would appreciate the help and see what we could possibly trade for. Much thanks and have a great week-end all! Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Controls for IF
What are people using to control direct IF? We use frozen tonsil for immunoglobulins and light chains, but what about fibrinogen, albumin, C1q, C3c and C4c? Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org www.NationwideChildrens.orghttp://www.nationwidechildrens.org/ - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Controls
We are in need of Aspergillus and Amyloid controls. Does anyone on the histonet have any suggestions, or overabundance that they could share with us? Thanks, Lorraine Cornett, HT (ASCP) Highlands Pathology Blue Ridge Division, Kingsport, TN 423 224-5793 fax 423 224-5349 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Controls with patient specimen on same slide
It is the most effective and economical way to run your IHC's. We have been doing it that way for as long as I have been in histology. Thanks, Tim - Original Message - From: histonet-requ...@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Tuesday, July 19, 2011 6:04 PM Subject: Histonet Digest, Vol 92, Issue 25 Hi, I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this? If you're not, why not? Thanks, Toni ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Controls with patient specimen on same slide
Just one. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, July 20, 2011 8:19 AM To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls with patient specimen on same slide All of these responses are great. So here's a follow up question. Do you place a control tissue on EACH slide if you have multiple blocks for a case, or just on one of the slides? -Original Message- From: Richard Cartun [mailto:rcar...@harthosp.org] Sent: Tuesday, July 19, 2011 6:40 PM To: histonet@lists.utsouthwestern.edu; Rathborne, Toni Subject: Re: [Histonet] Controls with patient specimen on same slide We do not put our positive control tissue on the test slide; we run batch controls. Many of the unstained slides (breast, GI, and prostate biopsies) that we use for IHC testing are cut in our Histology Laboratory as part of a part-type slide protocol. For example, we cut 7 slides, 2 sections on each slide, for breast biopsies and stain #1, 4, and 7 with HE, and then use (if needed) #2, 3, 5, and 6 for IHC. Therefore, it would be very difficult for us to place the positive control tissue on the same slide. In addition, I receive a lot of consult cases from other hospitals where they send us unstained slides for testing. Once again, it would be difficult to place the positive control tissue on the same slide and it would slow us down in terms of starting those slides once they arrive. However, I think the main reason we don't pursue putting the positive control tissue on the same slide is the fact that it would consume an enormous amount of control tissue. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Rathborne, Toni trathbo...@somerset-healthcare.com 7/19/2011 3:27 PM Hi, I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this? If you're not, why not? Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - This message was secured by ZixCorp(R). CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Controls with patient specimen on same slide
Each slide or else the whole theory of knowing each slide got treated the same goes out the window. :) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, July 20, 2011 9:19 AM To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls with patient specimen on same slide All of these responses are great. So here's a follow up question. Do you place a control tissue on EACH slide if you have multiple blocks for a case, or just on one of the slides? -Original Message- From: Richard Cartun [mailto:rcar...@harthosp.org] Sent: Tuesday, July 19, 2011 6:40 PM To: histonet@lists.utsouthwestern.edu; Rathborne, Toni Subject: Re: [Histonet] Controls with patient specimen on same slide We do not put our positive control tissue on the test slide; we run batch controls. Many of the unstained slides (breast, GI, and prostate biopsies) that we use for IHC testing are cut in our Histology Laboratory as part of a part-type slide protocol. For example, we cut 7 slides, 2 sections on each slide, for breast biopsies and stain #1, 4, and 7 with HE, and then use (if needed) #2, 3, 5, and 6 for IHC. Therefore, it would be very difficult for us to place the positive control tissue on the same slide. In addition, I receive a lot of consult cases from other hospitals where they send us unstained slides for testing. Once again, it would be difficult to place the positive control tissue on the same slide and it would slow us down in terms of starting those slides once they arrive. However, I think the main reason we don't pursue putting the positive control tissue on the same slide is the fact that it would consume an enormous amount of control tissue. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Rathborne, Toni trathbo...@somerset-healthcare.com 7/19/2011 3:27 PM Hi, I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this? If you're not, why not? Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - This message was secured by ZixCorp(R). CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please
Re: [Histonet] Controls with patient specimen on same slide
We do put controls on each slide in a case. Sometimes it's just one slide that failed in a run. A batch control wouldn't tell you which slide failed if there are no internal controls in the patient tissue. I personally wouldn't feel comfortable doing IHC with batch controls. Mark On Wed, Jul 20, 2011 at 6:18 AM, Rathborne, Toni trathbo...@somerset-healthcare.com wrote: All of these responses are great. So here's a follow up question. Do you place a control tissue on EACH slide if you have multiple blocks for a case, or just on one of the slides? -Original Message- From: Richard Cartun [mailto:rcar...@harthosp.org] Sent: Tuesday, July 19, 2011 6:40 PM To: histonet@lists.utsouthwestern.edu; Rathborne, Toni Subject: Re: [Histonet] Controls with patient specimen on same slide We do not put our positive control tissue on the test slide; we run batch controls. Many of the unstained slides (breast, GI, and prostate biopsies) that we use for IHC testing are cut in our Histology Laboratory as part of a part-type slide protocol. For example, we cut 7 slides, 2 sections on each slide, for breast biopsies and stain #1, 4, and 7 with HE, and then use (if needed) #2, 3, 5, and 6 for IHC. Therefore, it would be very difficult for us to place the positive control tissue on the same slide. In addition, I receive a lot of consult cases from other hospitals where they send us unstained slides for testing. Once again, it would be difficult to place the positive control tissue on the same slide and it would slow us down in terms of starting those slides once they arrive. However, I think the main reason we don't pursue putting the positive control tissue on the same slide is the fact that it would consume an enormous amount of control tissue. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Rathborne, Toni trathbo...@somerset-healthcare.com 7/19/2011 3:27 PM Hi, I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this? If you're not, why not? Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - This message was secured by ZixCorp(R). CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Controls with patient specimen on same slide
In our Lab we put a control on every slide. We keep multiple controls cut and stored in refrigerator. Most are multi- tissue controls. We use the Ventana detection kits and it is not cost efficient to use 2 uses of the detection kit per antibody. Of course there are times that this will not work, then our cost per test goes up, but we cannot charge any more for a control. (Embedded image moved to file: pic32000.jpg)___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Controls with patient specimen on same slide
Each case gets one positive control per antibody. If, for example, I run a bone marrow bx and aspirate, I use one positive control for both slides. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, July 20, 2011 9:19 AM To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls with patient specimen on same slide All of these responses are great. So here's a follow up question. Do you place a control tissue on EACH slide if you have multiple blocks for a case, or just on one of the slides? -Original Message- From: Richard Cartun [mailto:rcar...@harthosp.org] Sent: Tuesday, July 19, 2011 6:40 PM To: histonet@lists.utsouthwestern.edu; Rathborne, Toni Subject: Re: [Histonet] Controls with patient specimen on same slide We do not put our positive control tissue on the test slide; we run batch controls. Many of the unstained slides (breast, GI, and prostate biopsies) that we use for IHC testing are cut in our Histology Laboratory as part of a part-type slide protocol. For example, we cut 7 slides, 2 sections on each slide, for breast biopsies and stain #1, 4, and 7 with HE, and then use (if needed) #2, 3, 5, and 6 for IHC. Therefore, it would be very difficult for us to place the positive control tissue on the same slide. In addition, I receive a lot of consult cases from other hospitals where they send us unstained slides for testing. Once again, it would be difficult to place the positive control tissue on the same slide and it would slow us down in terms of starting those slides once they arrive. However, I think the main reason we don't pursue putting the positive control tissue on the same slide is the fact that it would consume an enormous amount of control tissue. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Rathborne, Toni trathbo...@somerset-healthcare.com 7/19/2011 3:27 PM Hi, I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this? If you're not, why not? Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - This message was secured by ZixCorp(R). CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Controls with patient specimen on same slide
Hi Toni, We put the control on the same slide and after it's stained and coverslipped we draw a line to seperate the control and patient tissue with each side of the line having either a C or P written on it. Mark On Tue, Jul 19, 2011 at 12:27 PM, Rathborne, Toni trathbo...@somerset-healthcare.com wrote: Hi, I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this? If you're not, why not? Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Controls with patient specimen on same slide
Whenever possible we put patient tissue on slides with positive control tissue on it. Everyone likes it and then you're assured your control and patient got the same staining experience. We used to use the red box control slides but discontinued due to the additional cost. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, July 19, 2011 2:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Controls with patient specimen on same slide Hi, I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this? If you're not, why not? Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Controls with patient specimen on same slide
We often do the same or use separate slides. We used to use the slides with the designated control area but had some problems with the reagents getting to all areas of the slide. Lisa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Tuesday, July 19, 2011 2:43 PM To: Rathborne, Toni Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Controls with patient specimen on same slide Hi Toni, We put the control on the same slide and after it's stained and coverslipped we draw a line to seperate the control and patient tissue with each side of the line having either a C or P written on it. Mark On Tue, Jul 19, 2011 at 12:27 PM, Rathborne, Toni trathbo...@somerset-healthcare.com wrote: Hi, I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this? If you're not, why not? Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Controls with patient specimen on same slide
We do not put our positive control tissue on the test slide; we run batch controls. Many of the unstained slides (breast, GI, and prostate biopsies) that we use for IHC testing are cut in our Histology Laboratory as part of a part-type slide protocol. For example, we cut 7 slides, 2 sections on each slide, for breast biopsies and stain #1, 4, and 7 with HE, and then use (if needed) #2, 3, 5, and 6 for IHC. Therefore, it would be very difficult for us to place the positive control tissue on the same slide. In addition, I receive a lot of consult cases from other hospitals where they send us unstained slides for testing. Once again, it would be difficult to place the positive control tissue on the same slide and it would slow us down in terms of starting those slides once they arrive. However, I think the main reason we don't pursue putting the positive control tissue on the same slide is the fact that it would consume an enormous amount of control tissue. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Rathborne, Toni trathbo...@somerset-healthcare.com 7/19/2011 3:27 PM Hi, I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this? If you're not, why not? Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Controls with patient specimen on same slide
We used to do batch controls too. And we cut unstained slides in a similar fashion also. In order to put the control on the already cut slide, one must stand on one's head or hang themselves upside down from the rafters, but it can be done.. Just put the slide in the water label end first and you have it! Took me years to get it going, and took awhile for techs to adjust, but now it's very routine. And if you use a derm punch you can get lots of control tissue from your blocks without destroying the block. And you save many dollars and give the pathologists assurance that the tissue was treated the same. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Tuesday, July 19, 2011 18:40 To: histonet@lists.utsouthwestern.edu; Toni Rathborne Subject: Re: [Histonet] Controls with patient specimen on same slide We do not put our positive control tissue on the test slide; we run batch controls. Many of the unstained slides (breast, GI, and prostate biopsies) that we use for IHC testing are cut in our Histology Laboratory as part of a part-type slide protocol. For example, we cut 7 slides, 2 sections on each slide, for breast biopsies and stain #1, 4, and 7 with HE, and then use (if needed) #2, 3, 5, and 6 for IHC. Therefore, it would be very difficult for us to place the positive control tissue on the same slide. In addition, I receive a lot of consult cases from other hospitals where they send us unstained slides for testing. Once again, it would be difficult to place the positive control tissue on the same slide and it would slow us down in terms of starting those slides once they arrive. However, I think the main reason we don't pursue putting the positive control tissue on the same slide is the fact that it would consume an enormous amount of control tissue. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Rathborne, Toni trathbo...@somerset-healthcare.com 7/19/2011 3:27 PM Hi, I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this? If you're not, why not? Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Controls for IPs
This response is late due to the overwhelming time commitments of NCAA Basketball Tournaments. The only multi-tissue control we use is for HER2 protein overexpression. In my opinion, multi-tissue controls are completely unnecessary for every-day use for the majority of IHC tests that we do. They are expensive and time-consuming to prepare and, as you pointed out, consume valuable tissue. Multi-tissue controls should be used during primary antibody optimization and validation studies; they are not needed for routine testing. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Bauer, Karen L. bauer.ka...@mayo.edu 3/8/2011 10:16 AM Hello, It seems we go back and forth between multi-tissue control blocks and single tissue control blocks when it comes to immunostaining. In the past we used single blocks and still continue to use some for the IPs that are rarely used. Then, switched to creating multi-tissue blocks for ease of use. It's so nice to cut control slides ahead of time and just pull them out and add our patient tissue. But... we find that the multi-tissue blocks are using up hard to find tissue. For example, we have a melanoma skin in one of the MTBs for the HMB45 and Melan A stains, but that MTB is used more for the CK7, CK20 and TTF-1 stains. Basically, that melanoma skin is just being wasted. I'm considering going back to the single block controls and was wondering what other labs are doing. What is the concensus in HistoLand in regards to IP control blocks/tissues? Thanks much!! Karen Karen L. Bauer HTL/HT (ASCP) Histology Supervisor Department of Pathology - Luther Hospital Luther Midelfort - Mayo Health System bauer.ka...@mayo.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CONTROLS
Hello everyone, I have an issue that I would like some help with. We are currently running SMM-HC and E-Cad on Breast cores and specimens from a derm lab. With the regulations being so strict regarding the processing times of breast specimens, how are you all dealing with that when it comes to your control tissue? We have to acquire breast tissue from outside sources to use for control, but it is very hard to get it within that 24-72 hour processing time since many hospitals will not release any tissue until the case has been signed out. Does anyone have any wonderful ideas on how to handle that? I would really appreciate it! Thanks histonetters. Sheila Knoxville Dermatopathology Lab Knoxville, TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Controls for IPs
Hello, It seems we go back and forth between multi-tissue control blocks and single tissue control blocks when it comes to immunostaining. In the past we used single blocks and still continue to use some for the IPs that are rarely used. Then, switched to creating multi-tissue blocks for ease of use. It's so nice to cut control slides ahead of time and just pull them out and add our patient tissue. But... we find that the multi-tissue blocks are using up hard to find tissue. For example, we have a melanoma skin in one of the MTBs for the HMB45 and Melan A stains, but that MTB is used more for the CK7, CK20 and TTF-1 stains. Basically, that melanoma skin is just being wasted. I'm considering going back to the single block controls and was wondering what other labs are doing. What is the concensus in HistoLand in regards to IP control blocks/tissues? Thanks much!! Karen Karen L. Bauer HTL/HT (ASCP) Histology Supervisor Department of Pathology - Luther Hospital Luther Midelfort - Mayo Health System bauer.ka...@mayo.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Controls needed
Does anyone out there in Histoland have a control block for MDM2 for liposarcoma that they would be willing to share? We don't get very many liposarcomas so control blocks are a problem. Any help is greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-dev...@carle.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Controls needed!
I second that. Marsha is the loveliest person I know. - Original Message - From: Denise Piontek dbpion...@hotmail.com To: histonet@lists.utsouthwestern.edu Sent: Wednesday, April 08, 2009 8:49 PM Subject: RE: [Histonet] Controls needed! Newcomer Supply has been a reliable control source, when necessary the have customized orders. Date: Wed, 8 Apr 2009 07:14:21 -0500 From: jennifer.l.hofec...@vanderbilt.edu To: dknut...@primecare.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls needed! CC: Hi Deanne, Have you checked with the NSH Control Tissue Bank? The form to request tissue is online on the NSH web page (www.nsh.org). The bank is run by the Quality Control committee in conjunction with the IHCRG. It is a free service to NSH members. You may contact the committee chair, William DeSalvo at wdesalvo@hotmail.com with any specific questions. Have a great week. Jennifer L. Hofecker HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph 615.343.0083 fax 615.343.7089 -Original Message- From: Knutson, Deanne [mailto:dknut...@primecare.org] Sent: Tuesday, April 07, 2009 3:06 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Controls needed! We are looking for H. Pylori control tissue and also GMS/fungus control tissue. Is there anyone out there that might have extra to share? We have good GRAM control blocks that we would be happy to exchange. Please let me know if you can help us out. Thank you! Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center Bismarck, N. Dak. 58506 701-530-6730 Fax 701-530-6735 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ Rediscover Hotmail®: Get quick friend updates right in your inbox. http://windowslive.com/RediscoverHotmail?ocid=TXT_TAGLM_WL_HM_Rediscover_Updates1_042009___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Controls needed!
Hi Deanne, Have you checked with the NSH Control Tissue Bank? The form to request tissue is online on the NSH web page (www.nsh.org). The bank is run by the Quality Control committee in conjunction with the IHCRG. It is a free service to NSH members. You may contact the committee chair, William DeSalvo at wdesalvo@hotmail.com with any specific questions. Have a great week. Jennifer L. Hofecker HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph 615.343.0083 fax 615.343.7089 -Original Message- From: Knutson, Deanne [mailto:dknut...@primecare.org] Sent: Tuesday, April 07, 2009 3:06 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Controls needed! We are looking for H. Pylori control tissue and also GMS/fungus control tissue. Is there anyone out there that might have extra to share? We have good GRAM control blocks that we would be happy to exchange. Please let me know if you can help us out. Thank you! Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center Bismarck, N. Dak. 58506 701-530-6730 Fax 701-530-6735 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Controls needed!
It's just a thought, can't you get the control from your Microbiology Lab? You just need to make a cell block out of the bacteria that they've grown; I'm not aware that Hpylori has to go through a human system before you can demonstrate it. Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hofecker, Jennifer L Sent: 08 April 2009 13:14 To: Knutson, Deanne; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls needed! Hi Deanne, Have you checked with the NSH Control Tissue Bank? The form to request tissue is online on the NSH web page (www.nsh.org). The bank is run by the Quality Control committee in conjunction with the IHCRG. It is a free service to NSH members. You may contact the committee chair, William DeSalvo at wdesalvo@hotmail.com with any specific questions. Have a great week. Jennifer L. Hofecker HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph 615.343.0083 fax 615.343.7089 -Original Message- From: Knutson, Deanne [mailto:dknut...@primecare.org] Sent: Tuesday, April 07, 2009 3:06 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Controls needed! We are looking for H. Pylori control tissue and also GMS/fungus control tissue. Is there anyone out there that might have extra to share? We have good GRAM control blocks that we would be happy to exchange. Please let me know if you can help us out. Thank you! Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center Bismarck, N. Dak. 58506 701-530-6730 Fax 701-530-6735 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Controls needed!
We are looking for H. Pylori control tissue and also GMS/fungus control tissue. Is there anyone out there that might have extra to share? We have good GRAM control blocks that we would be happy to exchange. Please let me know if you can help us out. Thank you! Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center Bismarck, N. Dak. 58506 701-530-6730 Fax 701-530-6735 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
