[Histonet] Cryostat help
Hello Histonetters, I am new to the histology world and I was hoping to get some feedback about staining after cutting sections in the cryostat. Does anyone have a protocol they use for staining after sectioning tissue in a cryostat? I have never done it before so I thought I would come to the experts. Thanks for your help! Kimberly ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryostat help
That depends on what staining you need to use. If an IHC, just fix the sections in acetone, air dry and proceed without HIER If HE fix quickly with formalin 10%, wash → hematoxylin for 30 secs without differentiation → eosin 15 sec→ dehydrate and mount. Other HC procedures all will require fixation and using the specific protocol. Always remember that it is a frozen section and therefore more delicate and less adhered than a paraffin section. René J. --- On Tue, 1/11/11, Blundon, Kimberly kimberly.blun...@drdc-rddc.gc.ca wrote: From: Blundon, Kimberly kimberly.blun...@drdc-rddc.gc.ca Subject: [Histonet] Cryostat help To: histonet@lists.utsouthwestern.edu Date: Tuesday, January 11, 2011, 10:28 AM Hello Histonetters, I am new to the histology world and I was hoping to get some feedback about staining after cutting sections in the cryostat. Does anyone have a protocol they use for staining after sectioning tissue in a cryostat? I have never done it before so I thought I would come to the experts. Thanks for your help! Kimberly ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cryostat help
We have a similar frozen section protocol but we fix the slide in 95% alcohol for ~1minute and then stain with Gill's Hematoxylin for 1 minute, rinse, blue and then alcohol, foloowed by Eosin for ~ 15secs.- dehydrate and mount. Lisa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, January 11, 2011 10:29 AM To: histonet@lists.utsouthwestern.edu; KimberlyBlundon Subject: Re: [Histonet] Cryostat help That depends on what staining you need to use. If an IHC, just fix the sections in acetone, air dry and proceed without HIER If HE fix quickly with formalin 10%, wash → hematoxylin for 30 secs without differentiation → eosin 15 sec→ dehydrate and mount. Other HC procedures all will require fixation and using the specific protocol. Always remember that it is a frozen section and therefore more delicate and less adhered than a paraffin section. René J. --- On Tue, 1/11/11, Blundon, Kimberly kimberly.blun...@drdc-rddc.gc.ca wrote: From: Blundon, Kimberly kimberly.blun...@drdc-rddc.gc.ca Subject: [Histonet] Cryostat help To: histonet@lists.utsouthwestern.edu Date: Tuesday, January 11, 2011, 10:28 AM Hello Histonetters, I am new to the histology world and I was hoping to get some feedback about staining after cutting sections in the cryostat. Does anyone have a protocol they use for staining after sectioning tissue in a cryostat? I have never done it before so I thought I would come to the experts. Thanks for your help! Kimberly ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cryostat help please!
Dear Mohamed, We manufacture cryostats but not the one you have the problem with, however I think what you can hear is the cooling fan working but not the refrigeration compressor. You will need to locate the internal fuses to see which ones need replacing. If the compressor is running then it would seem to have lost its refrigeration gas. Unfortunately I am leaving my office now and Histonet does not seem to like my Blackberry, but if you would like to talk further on this problem you can catch me on Skype dazzle0 later today or let me have your phone number and I will call you. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abri...@brightinstruments.com Web Site: www.brightinstruments.com Skype: dazzle0 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik Sent: 20 March 2010 13:31 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat help please! hi all histoneters i have learned alot from your posts and i need any suggestion about a cryostat that was working perfectly until the power cable is pluged off accidently. then after about 2 days i put it again in the power and all is working with its motor sound but no refregration and room temp. not decrease under 20 degrees!!! i let it for aweek but no change! unfortene the technical service may take long time till it come to see the proplem as it is in a national university ( routine work) and i hope i could do that. any suggestion please? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cryostat help please!
Sounds like it may be a fuse... in my non-expert opinion!! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu on behalf of mohamed abd el razik Sent: Sat 3/20/2010 9:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat help please! hi all histoneters i have learned alot from your posts and i need any suggestion about a cryostat that was working perfectly until the power cable is pluged off accidently. then after about 2 days i put it again in the power and all is working with its motor sound but no refregration and room temp. not decrease under 20 degrees!!! i let it for aweek but no change! unfortene the technical service may take long time till it come to see the proplem as it is in a national university ( routine work) and i hope i could do that. any suggestion please? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] cryostat help please!
our cryostat is Minotome Plus Microtome Cryostat Cat. No. 2563-- For 120 VAC, 60Hz --- On Sat, 3/20/10, Cheryl Cornett-Early cornettear...@bellsouth.net wrote: From: Cheryl Cornett-Early cornettear...@bellsouth.net Subject: Re: [Histonet] cryostat help please! To: mohamed abd el razik k8...@yahoo.com Date: Saturday, March 20, 2010, 10:59 PM Hi Mohamed, I AM a Services guy and this problem sounds like a loss of refrigerant I come across this problem on older Cryostat's after they have been turned off regularly, What model of cryostat is it?. IT sounds like a CM1800 Riechet Jung or (Leica). Best regards Brian. My territory is the south east, GA, AL, TN, SC, NC, --- On Sat, 3/20/10, mohamed abd el razik k8...@yahoo.com wrote: From: mohamed abd el razik k8...@yahoo.com Subject: [Histonet] cryostat help please! To: histonet@lists.utsouthwestern.edu Date: Saturday, March 20, 2010, 9:30 AM hi all histoneters i have learned alot from your posts and i need any suggestion about a cryostat that was working perfectly until the power cable is pluged off accidently. then after about 2 days i put it again in the power and all is working with its motor sound but no refregration and room temp. not decrease under 20 degrees!!! i let it for aweek but no change! unfortene the technical service may take long time till it come to see the proplem as it is in a national university ( routine work) and i hope i could do that. any suggestion please? Histonet@lists.utsouthwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cryostat help please!
I agree with Joyce, it sounds like the fuse for the refrigeration. I had that problem a few times. Robyn Vazquez Date: Sun, 21 Mar 2010 08:28:36 -0400 From: jwe...@sjha.org To: k8...@yahoo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cryostat help please! CC: Sounds like it may be a fuse... in my non-expert opinion!! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu on behalf of mohamed abd el razik Sent: Sat 3/20/2010 9:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat help please! hi all histoneters i have learned alot from your posts and i need any suggestion about a cryostat that was working perfectly until the power cable is pluged off accidently. then after about 2 days i put it again in the power and all is working with its motor sound but no refregration and room temp. not decrease under 20 degrees!!! i let it for aweek but no change! unfortene the technical service may take long time till it come to see the proplem as it is in a national university ( routine work) and i hope i could do that. any suggestion please? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cryostat Help Please
On behalf of mohamed abd el razik QUESTION: hi all histoneters i have learned alot from your posts and i need any suggestion about a cryostat that was working perfectly until the power cable is pluged off accidently. then after about 2 days i put it again in the power and all is working with its motor sound but no refregration and room temp. not decrease under 20 degrees!!! i let it for aweek but no change! unfortene the technical service may take long time till it come to see the proplem as it is in a national university ( routine work) and i hope i could do that. any suggestion please? MY ANSWER: With the cryostat plugged into power, I would recycle power to the unit by turning the power switch to off, wait a couple seconds, and then return the power switch to on. This could possibly reset the logic in the units power circuit. Just a thought... ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cryostat help please!
hi all histoneters i have learned alot from your posts and i need any suggestion about a cryostat that was working perfectly until the power cable is pluged off accidently. then after about 2 days i put it again in the power and all is working with its motor sound but no refregration and room temp. not decrease under 20 degrees!!! i let it for aweek but no change! unfortene the technical service may take long time till it come to see the proplem as it is in a national university ( routine work) and i hope i could do that. any suggestion please? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] cryostat help please!
thanks for your replay ray yes i set the temp. to -20 degrees but it is 20 for the last week. --- On Sat, 3/20/10, Mark Ray darkd...@comcast.net wrote: From: Mark Ray darkd...@comcast.net Subject: Re: [Histonet] cryostat help please! To: mohamed abd el razik k8...@yahoo.com Date: Saturday, March 20, 2010, 5:03 PM Are you sure the temperature control is set properly? This maybe a serious problem, but it is possible that the people who do the ordinary repairs on refrigeration equipment for the university can repair it. You should ask them if they can help you. mohamed abd el razik wrote: hi all histoneters i have learned alot from your posts and i need any suggestion about a cryostat that was working perfectly until the power cable is pluged off accidently. then after about 2 days i put it again in the power and all is working with its motor sound but no refregration and room temp. not decrease under 20 degrees!!! i let it for aweek but no change! unfortene the technical service may take long time till it come to see the proplem as it is in a national university ( routine work) and i hope i could do that. any suggestion please? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
