[Histonet] Histonet] Freezing spray in cryostats

2019-09-26 Thread White, Lisa M. via Histonet 
We did use freeze spray in the cryostat years ago.  Then one fateful day we all 
had to go on protocol for exposure to TB because a resident sprayed a lung with 
the freeze spray and there you go.  There is a turn around time for frozen 
sections we are all working with, HOWEVER it is not worth the health and safety 
of Staff to rush the process.  Use the heat extractor and most cryostats have a 
"turbo" cool bar.  Safety first or no one will be left to run the lab.
___
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Re: [Histonet] Freezing spray in cryostats

2019-09-25 Thread Akemi via Histonet 
I make my tech’s wear PPE’s, goggles/masks, gloves and N95 masks for potential 
TB and regular masks for normal tissue.
Akemi Allison

Sent from my iPhone

> On Sep 25, 2019, at 1:00 PM, Charles Riley via Histonet 
>  wrote:
> 
> I have to agree with Terri. I also believe that CAP is going to be putting
> in regulations forbidding the use of freeze sprays in cryostats soon (at
> least I think I heard a rumor about this).
> 
> Anyway another useful item is liquid nitrogen if you really need a quick
> freeze. Dip a brush into the liquid and lightly apply to the specimen. Not
> always recommended as could cause artifacts.
> 
> On Wed, Sep 25, 2019 at 1:37 PM Terri Braud via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
> 
>> My question is why would you ever want to use an aerosol in the cryostat,
>> taking a chance on aerosolizing some nasty bug?
>> Is the 30 seconds you save in using freezing spray worth the exposure to
>> TB or goodness-knows-what?
>> We use a simple metal heat extractor (comes standard in our Leicas) and
>> place it on top of the freezing block to accelerate the process.  We never
>> have issues with turn around times.
>> For me, no freezing spray.  Ever.
>> 
>> Terri L. Braud, HT(ASCP)
>> Anatomic Pathology Supervisor
>> Laboratory
>> Holy Redeemer Hospital
>> 1648 Huntingdon Pike
>> Meadowbrook, PA 19046
>> ph: 215-938-3689
>> fax: 215-938-3874
>> Care, Comfort, and Heal
>> 
>> -Original Message-
>> From: histonet-requ...@lists.utsouthwestern.edu [mailto:
>> histonet-requ...@lists.utsouthwestern.edu]
>> Sent: Wednesday, September 25, 2019 1:00 PM
>> To: histonet@lists.utsouthwestern.edu
>> Subject: Histonet Digest, Vol 190, Issue 18
>> 
>> CAUTION: This is an EXTERNAL EMAIL. Stop and think before clicking links
>> or opening attachments.
>> 
>> Send Histonet mailing list submissions to
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>> 
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>> 
>> When replying, please edit your Subject line so it is more specific
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>> 
>> 
>> Today's Topics:
>> 
>>   1. Flammable Sprays in Cryostats (Knutson, Deanne)
>>   2. Re: Flammable Sprays in Cryostats (Akemi)
>>   3. Re: Flammable Sprays in Cryostats (Hannen, Valerie)
>>   4. Re: Flammable Sprays in Cryostats (Ingles Claire)
>> 
>> 
>> --
>> 
>> Message: 1
>> Date: Wed, 25 Sep 2019 07:50:44 -0500
>> From: "Knutson, Deanne" 
>> To: "'histonet@lists.utsouthwestern.edu'"
>>
>> Subject: [Histonet] Flammable Sprays in Cryostats
>> Message-ID:
>><
>> 1e0e2b14c709174b8ac2be0ae7f768330159b5756...@exchange2k7.staprimecare.org>
>> 
>> Content-Type: text/plain; charset="us-ascii"
>> 
>> I just received a letter from Leica Biosystems where they are prohibiting
>> the usage of flammable
>> freezing sprays in their cryostats.  What are others using in their
>> cryostats to instantly freeze specimens?
>> 
>> Thank you.
>> 
>> 
>> Deanne Knutson
>> Supervisor
>> Anatomic Pathology
>> dknut...@primecare.org
>> 
>> 
>> 
>> 
>> 
>> 
>>  
>> This email may include confidential and privileged information. If this is
>> not intended for your use, please destroy immediately and contact the
>> sender of the message.
>> 
>> This email and attachments contain information that may be confidential or
>> privileged. If you are not the intended recipient, notify the sender at
>> once and delete this message completely from your information system.
>> Further use, disclosure, or copying of information contained in this email
>> is not authorized, and any such action should not be construed as a waiver
>> of privilege or other confidentiality protections.
>> 
>> 
>> --
>> 
>> Message: 2
>> Date: Wed, 25 Sep 2019 09:05:20 -0500
>> From: Akemi 
>> To: "Knutson, Deanne" , Histonet
>>
>> Subject: Re: [Histonet] Flammable Sprays in Cryostats
>> Message-ID: <19db25eb-9944-406b-917c-0878d6f15...@gmail.com>
>> Content-Type: text/plain;   charset=us-ascii
>> 
>> We are purchasing two of their newer UV cryostats and they were adamant
>> that we use a non flammable freezing spray because it can damage the UV
>> unit and have potential for exploding.
>> 
>> Akemi Allison
>> 
>> Sent from my iPhone
>> 
>>> On Sep 25, 2019, at 7:50 AM, Knutson, Deanne via Histonet <
>> histonet@lists.utsouthwestern.edu> wrote:
>>> 
>>> I just received a letter from Leica Biosystems where they are
>> prohibiting the usage of flammable
>>> freezing sprays in their cryostats.  What are others using in their
>> cryostats to instantly f

Re: [Histonet] Freezing spray in cryostats

2019-09-25 Thread Charles Riley via Histonet 
I have to agree with Terri. I also believe that CAP is going to be putting
in regulations forbidding the use of freeze sprays in cryostats soon (at
least I think I heard a rumor about this).

Anyway another useful item is liquid nitrogen if you really need a quick
freeze. Dip a brush into the liquid and lightly apply to the specimen. Not
always recommended as could cause artifacts.

On Wed, Sep 25, 2019 at 1:37 PM Terri Braud via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> My question is why would you ever want to use an aerosol in the cryostat,
> taking a chance on aerosolizing some nasty bug?
> Is the 30 seconds you save in using freezing spray worth the exposure to
> TB or goodness-knows-what?
> We use a simple metal heat extractor (comes standard in our Leicas) and
> place it on top of the freezing block to accelerate the process.  We never
> have issues with turn around times.
> For me, no freezing spray.  Ever.
>
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supervisor
> Laboratory
> Holy Redeemer Hospital
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> ph: 215-938-3689
> fax: 215-938-3874
> Care, Comfort, and Heal
>
> -Original Message-
> From: histonet-requ...@lists.utsouthwestern.edu [mailto:
> histonet-requ...@lists.utsouthwestern.edu]
> Sent: Wednesday, September 25, 2019 1:00 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: Histonet Digest, Vol 190, Issue 18
>
> CAUTION: This is an EXTERNAL EMAIL. Stop and think before clicking links
> or opening attachments.
>
> Send Histonet mailing list submissions to
> histonet@lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
> histonet-requ...@lists.utsouthwestern.edu
>
> You can reach the person managing the list at
> histonet-ow...@lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
>1. Flammable Sprays in Cryostats (Knutson, Deanne)
>2. Re: Flammable Sprays in Cryostats (Akemi)
>3. Re: Flammable Sprays in Cryostats (Hannen, Valerie)
>4. Re: Flammable Sprays in Cryostats (Ingles Claire)
>
>
> --
>
> Message: 1
> Date: Wed, 25 Sep 2019 07:50:44 -0500
> From: "Knutson, Deanne" 
> To: "'histonet@lists.utsouthwestern.edu'"
> 
> Subject: [Histonet] Flammable Sprays in Cryostats
> Message-ID:
> <
> 1e0e2b14c709174b8ac2be0ae7f768330159b5756...@exchange2k7.staprimecare.org>
>
> Content-Type: text/plain; charset="us-ascii"
>
> I just received a letter from Leica Biosystems where they are prohibiting
> the usage of flammable
> freezing sprays in their cryostats.  What are others using in their
> cryostats to instantly freeze specimens?
>
> Thank you.
>
>
> Deanne Knutson
> Supervisor
> Anatomic Pathology
> dknut...@primecare.org
>
>
>
>
>
>
>   
> This email may include confidential and privileged information. If this is
> not intended for your use, please destroy immediately and contact the
> sender of the message.
>
> This email and attachments contain information that may be confidential or
> privileged. If you are not the intended recipient, notify the sender at
> once and delete this message completely from your information system.
> Further use, disclosure, or copying of information contained in this email
> is not authorized, and any such action should not be construed as a waiver
> of privilege or other confidentiality protections.
>
>
> --
>
> Message: 2
> Date: Wed, 25 Sep 2019 09:05:20 -0500
> From: Akemi 
> To: "Knutson, Deanne" , Histonet
> 
> Subject: Re: [Histonet] Flammable Sprays in Cryostats
> Message-ID: <19db25eb-9944-406b-917c-0878d6f15...@gmail.com>
> Content-Type: text/plain;   charset=us-ascii
>
> We are purchasing two of their newer UV cryostats and they were adamant
> that we use a non flammable freezing spray because it can damage the UV
> unit and have potential for exploding.
>
> Akemi Allison
>
> Sent from my iPhone
>
> > On Sep 25, 2019, at 7:50 AM, Knutson, Deanne via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
> >
> > I just received a letter from Leica Biosystems where they are
> prohibiting the usage of flammable
> > freezing sprays in their cryostats.  What are others using in their
> cryostats to instantly freeze specimens?
> >
> > Thank you.
> >
> >
> > Deanne Knutson
> > Supervisor
> > Anatomic Pathology
> > dknut...@primecare.org
> >
> >
> >
> >
> >
> >
> >  
> > This email may include confidential and privileged information. If this
> is not intended for your use, please destroy immediately and contact the
> sender of the me

Re: [Histonet] Freezing spray in cryostats

2019-09-25 Thread Terri Braud via Histonet 
My question is why would you ever want to use an aerosol in the cryostat, 
taking a chance on aerosolizing some nasty bug?
Is the 30 seconds you save in using freezing spray worth the exposure to TB or 
goodness-knows-what?  
We use a simple metal heat extractor (comes standard in our Leicas) and place 
it on top of the freezing block to accelerate the process.  We never have 
issues with turn around times.
For me, no freezing spray.  Ever.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal

-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu 
[mailto:histonet-requ...@lists.utsouthwestern.edu] 
Sent: Wednesday, September 25, 2019 1:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 190, Issue 18

CAUTION: This is an EXTERNAL EMAIL. Stop and think before clicking links or 
opening attachments.

Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-requ...@lists.utsouthwestern.edu

You can reach the person managing the list at
histonet-ow...@lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."


Today's Topics:

   1. Flammable Sprays in Cryostats (Knutson, Deanne)
   2. Re: Flammable Sprays in Cryostats (Akemi)
   3. Re: Flammable Sprays in Cryostats (Hannen, Valerie)
   4. Re: Flammable Sprays in Cryostats (Ingles Claire)


--

Message: 1
Date: Wed, 25 Sep 2019 07:50:44 -0500
From: "Knutson, Deanne" 
To: "'histonet@lists.utsouthwestern.edu'"

Subject: [Histonet] Flammable Sprays in Cryostats
Message-ID:

<1e0e2b14c709174b8ac2be0ae7f768330159b5756...@exchange2k7.staprimecare.org>

Content-Type: text/plain; charset="us-ascii"

I just received a letter from Leica Biosystems where they are prohibiting the 
usage of flammable
freezing sprays in their cryostats.  What are others using in their cryostats 
to instantly freeze specimens?

Thank you.


Deanne Knutson
Supervisor
Anatomic Pathology
dknut...@primecare.org






  
This email may include confidential and privileged information. If this is not 
intended for your use, please destroy immediately and contact the sender of the 
message.

This email and attachments contain information that may be confidential or 
privileged. If you are not the intended recipient, notify the sender at once 
and delete this message completely from your information system. Further use, 
disclosure, or copying of information contained in this email is not 
authorized, and any such action should not be construed as a waiver of 
privilege or other confidentiality protections.


--

Message: 2
Date: Wed, 25 Sep 2019 09:05:20 -0500
From: Akemi 
To: "Knutson, Deanne" , Histonet

Subject: Re: [Histonet] Flammable Sprays in Cryostats
Message-ID: <19db25eb-9944-406b-917c-0878d6f15...@gmail.com>
Content-Type: text/plain;   charset=us-ascii

We are purchasing two of their newer UV cryostats and they were adamant that we 
use a non flammable freezing spray because it can damage the UV unit and have 
potential for exploding.

Akemi Allison

Sent from my iPhone

> On Sep 25, 2019, at 7:50 AM, Knutson, Deanne via Histonet 
>  wrote:
> 
> I just received a letter from Leica Biosystems where they are prohibiting the 
> usage of flammable
> freezing sprays in their cryostats.  What are others using in their cryostats 
> to instantly freeze specimens?
> 
> Thank you.
> 
> 
> Deanne Knutson
> Supervisor
> Anatomic Pathology
> dknut...@primecare.org
> 
> 
> 
> 
> 
> 
>  
> This email may include confidential and privileged information. If this is 
> not intended for your use, please destroy immediately and contact the sender 
> of the message.
> 
> This email and attachments contain information that may be confidential or 
> privileged. If you are not the intended recipient, notify the sender at once 
> and delete this message completely from your information system. Further use, 
> disclosure, or copying of information contained in this email is not 
> authorized, and any such action should not be construed as a waiver of 
> privilege or other confidentiality protections.
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet



--

Message: 3
Date: Wed, 25 Sep 2019 15:49:00 +
From: "Hannen, Valerie"