[Histonet] H for H. pylori

[Cassie P. Davis via Histonet]

In the beginning of my last FT job we had a excellent female pathologist who 
could see the H. pylori on SurgiPath's  H stain. I learned so much from her 
and dearly missed her toward the end.

Cassandra Davis
Histology Technician
AP Laboratory
302-575-8095
Email:  cda...@che-east.org



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Re: [Histonet] H for H. pylori?

[Tony Henwood (SCHN) via Histonet]

The following might be useful:

Tazawa, K., & Tsutsumi, Y. (1998). Effect of prolonged staining with 
hematoxylin on detecting Helicobacter pyiori in hematoxylin‐eosin‐stained 
gastric mucosa. Pathology international, 48(6), 448-452.


Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Principal Scientist, the Children’s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 



-Original Message-
From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, 10 October 2018 6:46 AM
To: P Sicurello; HistoNet
Subject: Re: [Histonet] H for H. pylori?

Paula:"Optimizing" H to detect H. piloris is a rout you should not attempt 
because H cannot be "optimized" for that.Giemsa is one way or, even better, 
modified Steiner stain. The only disadvantage modified Steiner has is using 
radioactive uranium nitrate, but I developed a procedure where 0.02% aq. 
phosphotungstic acid solution can be used instead. IHC will be way more 
expensive.
René 

On Tuesday, October 9, 2018 2:03 PM, P Sicurello via Histonet 
 wrote:
 

 Good Morning Listers,

Has anyone out there optimized an H for H. pylori?

Sure we can run a giemsa or and IHC but what fun is that?

Send me your ideas and make the day of our GI pathologists.

Thanks oodles.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

9300 Campus Point Drive

La Jolla, CA 92037
(P): 858-249-5610



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Re: [Histonet] H for H. pylori?

[Rene J Buesa via Histonet]

Paula:"Optimizing" H to detect H. piloris is a rout you should not attempt 
because H cannot be "optimized" for that.Giemsa is one way or, even better, 
modified Steiner stain. The only disadvantage modified Steiner has is using 
radioactive uranium nitrate, but I developed a procedure where 0.02% aq. 
phosphotungstic acid solution can be used instead. IHC will be way more 
expensive.
René 

On Tuesday, October 9, 2018 2:03 PM, P Sicurello via Histonet 
 wrote:
 

 Good Morning Listers,

Has anyone out there optimized an H for H. pylori?

Sure we can run a giemsa or and IHC but what fun is that?

Send me your ideas and make the day of our GI pathologists.

Thanks oodles.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

9300 Campus Point Drive

La Jolla, CA 92037
(P): 858-249-5610



*Confidentiality Notice*: The information transmitted in this e-mail is
intended only for the person or entity to which it is addressed and may
contain confidential and/or privileged material.  Any review,
retransmission, dissemination or other use of or taking of any action in
reliance upon this information by persons or entities other than the
intended recipient is prohibited.  If you received this e-mail in error,
please contact the sender and delete the material from any computer.
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[Histonet] H for H. pylori?

[P Sicurello via Histonet]

Good Morning Listers,

Has anyone out there optimized an H for H. pylori?

Sure we can run a giemsa or and IHC but what fun is that?

Send me your ideas and make the day of our GI pathologists.

Thanks oodles.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

9300 Campus Point Drive

La Jolla, CA 92037
(P): 858-249-5610



*Confidentiality Notice*: The information transmitted in this e-mail is
intended only for the person or entity to which it is addressed and may
contain confidential and/or privileged material.  Any review,
retransmission, dissemination or other use of or taking of any action in
reliance upon this information by persons or entities other than the
intended recipient is prohibited.  If you received this e-mail in error,
please contact the sender and delete the material from any computer.
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